Integra VACUSIP User manual

VACUSIP Operating instructions
159950_V04

Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
Zizers, November 4, 2016
Description Models
VACUSIP 159000, 159010
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Battery Directive 2006/66/EC
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) 1907/2006
Capacity Labelling of Portable Secondary Batteries 1103/2010
Ecodesign - Power supplies 278/2009
Standards for EU
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61010-1: 2010
Electrical equipment for measurement, control and laboratory use -
EMC requirements.
EN 61326-1: 2013
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
CAN/CSA-C22.2
No. 61010-1
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
UL 61010-1
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Part 15 of the
FCC Rules
Class A
Elmar Morscher
CEO
Thomas Neher
Quality Manager

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Table of Contents
1 Introduction ................................................................................................................4
2 Description of the device ............................................................................................6
3 Installation ..................................................................................................................7
4 Operation ...................................................................................................................9
5 Maintenance .............................................................................................................11
6 Technical data ..........................................................................................................12
7 Accessories and consumables .................................................................................14
Imprint
© 2018 INTEGRA Biosciences AG
Manufacturer
Customer service
Please contact your local INTEGRA Biosciences representative, see
www.integra-biosciences.com or contact [e-mail].
INTEGRA BIOSCIENCES AG
CH-7205 Zizers, Suisse
T [phone]
F [phone]
INTEGRA BIOSCIENCES CORP.
Hudson, NH 03051, USA
T [phone]
F [phone]
[e-mail]
www.integra-biosciences.com

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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the VACUSIP aspiration system.
1.1 Intended use
This is a general-purpose laboratory instrument. Any use of this instrument in a medical
or IVD setting is under the sole responsibility of the user.
VACUSIP is a vacuum-based system for aspiration of non explosive aqueous solutions,
such as biological buffers and media.
If the VACUSIP is used in a manner not specified by INTEGRA Biosciences, the protection
provided by the VACUSIP may be impaired.
1.2 Safety notes
WARNING
Read these operating instructions carefully before use and pay particular
attention to sections containing this symbol.
1.2.1 General
1) Do not carry out any conversions and alterations on the device.
2) Defective parts may only be replaced with original INTEGRA Biosciences spare parts
according to the INTEGRA Biosciences operating or service instructions.
3) Do not use or charge VACUSIP in an atmosphere with danger of explosion. Also, do
not aspirate highly flammable liquids such as acetone or ether.
4) When handling dangerous substances, comply with the material safety data sheet
(MSDS) and with all safety guidelines such as the use of protective clothing and safety
goggles.
5) Prolonged exposure of VACUSIP to UV-light can cause discoloration and/or yellowing
of the plastic housing. However, this will not affect the performance of the device in any
way.
6) Excess spare parts, accessories and consumables (e.g. tubings, lids, plastic parts,
rubber products, O-rings, filter) should be stored in a light protected place at ambient
temperature to prevent premature material aging caused by prolonged exposure to UV-
light.
Regardless of the listed safety notes, additionally applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

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1.2.2 Battery (model 159 000)
1) Charge the VACUSIP device using only the charger supplied by INTEGRA
Biosciences.
2) Old Li-ion batteries may cause a safety risk. We recommend to replace the battery
after 3 years of use. Also replace the battery if the charging intervals are unusually
short or if the charging takes much longer than usual (6 hours or more). These are
indicators that the battery has reached the end of its life-cycle.
3) Li-ion technology bears the risk of thermal runaway and cell rupture if the battery was
damaged. Do not expose the battery to heat (> 60°C) and avoid mechanical stress.
Batteries which were subject to deep discharges may develop internal short circuits,
leading to an increased self-discharge rate and heating during battery charging. This
may also result in thermal runaway and cell rupture.
4) To extend the battery life-cycle, it is recommended to charge the battery every 2
months if the VACUSIP is not used regularly. If the VACUSIP is not used for more than
6 months, unplug the battery, see 3.3.5.
5) If despite all safety measures the VACUSIP Li-ion battery catches fire and toxic
(typically dense, white) fumes emanate from the VACUSIP device, immediately
evacuate the working area. The VACUSIP housing is made of special flame retardant
material that has proven its capability to contain a battery fire. During a battery fire the
housing surface may become very hot. Do not touch the housing by hand and wait at
least 20 minutes after fumes have disappeared before approaching the device. Do no
longer use the VACUSIP device.

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2 Description of the device
Scope of delivery
• VACUSIP base unit without battery (model 159 010) or with battery (model 159 000)
• Mains adapter
• INTEGRA glass bottle 500 ml with standard GL 45 lid
• GL 45 lid with tube fittings
• Tubing and hydrophobic filter with an additional spare filter
• VACUSIP hand operator
• 8-channel stainless steel tips
• 1-channel stainless steel tip
• 1-channel adapter for disposable tips
Rear view
1 On/off switch
2 Indicator LED
3 Base unit
4 Stainless steel rod
5 VACUSIP hand operator
6 Hand operator mount
7 Lid with tube fittings
8 500 ml bottle for liquid collection
9 Hydrophobic filter
10 Silicone filter adapter
11 Air outlet
12 Opening to insert stainless steel rod
13 Mains connection socket
1
3
2
4
5
6
7
8
13
9
10
11
12

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3 Installation
3.1 Power supply
Insert the mains adapter cable into the VACUSIP mains connection socket (13) and plug
it into the power source.
WARNING
Use an original INTEGRA Biosciences mains adapter only (see “6.1
Specifications” on page 12 for voltage requirements)
3.2 Charging of VACUSIP battery model
A full charge takes 5 hours. Before the first use, VACUSIP should be charged for at least
5 hours.
When the LED (2) starts to blink green, the battery needs to be recharged immediately to
prolong battery life. VACUSIP has an integrated overcharge protection.
VACUSIP can be used while it is being charged.
3.3 Set-up
3.3.1 Installing the VACUSIP hand operator mount
Find the enclosed stainless steel rod (4) and push it into the opening (12).
Attach the hand operator mount (6) to the top of the stainless steel rod (4).
WARNING
Do not carry VACUSIP by holding the rod or the hand operator mount. Always
grab the instrument at the back to carry it around.
3.3.2 Assembling the hand operator
3.3.3 Connecting the filter
Attach the shorter silicone tubing to the filter (9) and then plug the filter into the silicone
filter adapter with the blue side facing the instrument.
VACUSIP should never be used without a filter.
Insert the inner part in the silicone
cover, as shown on the picture
beside.

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3.3.4 Connecting the silicone tubing
Place the bottle in the instrument and screw on the green lid with tube fittings (7).
Attach the silicone tubing coming from the filter to the fitting on the lid named “FILTER”.
Attach the longer silicone tubing to the fitting in the lid named “LIQ” (liquid) and the other
end to the VACUSIP hand operator (5).
3.3.5 Replacing the battery
Unscrew the 5 screws on the bottom of the device with a
Phillips screwdriver nr. 1 and remove the bottom lid.
Unplug the old battery and replace it with an original
INTEGRA Biosciences rechargeable battery, see “7
Accessories and consumables” on page 14. Make sure that
it is inserted with the correct polarity (+/-).
Place back the battery in the battery compartment with the
wire facing to the inside of the instrument.
Close the bottom lid and fix it with the 5 screws.

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4 Operation
4.1 Vacuum activation and notes on use
Switch on VACUSIP to start the vacuum pump (the LED lights up green).
Once the vacuum is reached, the pump stops until the vacuum falls below threshold.
VACUSIP switches off automatically after the pump has not been running for 10 minutes
or 40 minutes after initial switch-on. This feature can be temporarily deactivated by
pressing the power button for at least 4 seconds when switching on the instrument. The
power-off function is automatically restored after turning off VACUSIP.
Check the level of liquid in the bottle frequently. The liquid level should never reach the lid.
Prevent liquid or foam overflow from the collection bottle into the filter. In case the filter
gets accidentally wetted or soiled, exchange it immediately or otherwise the retention of
further liquid cannot be guaranteed. Note that the hydrophobic filter holds back aqueous
solutions, whereas retention of apolar liquids is limited.
WARNING
When working with dangerous substances, you must comply with the material
safety data sheet (MSDS) and the additionally applicable regulations of trade
associations, health authorities, supervisory offices, etc. In addition, when
working with hazardous volatile substances or biohazardous agents, either
place the VACUSIP inside a biosafety cabinet or connect the instrument's air
outlet (11) to a safety ventilation by means of a tube (ID=4 mm) and an
aspiration needle (Part. No. 155 502).
4.2 Code of indicator LED
Attach a suitable adapter to the hand operator.
To aspirate press the soft buttons on both sides of the
VACUSIP hand operator.
LED code Meaning
Constantly green Unit is powered on and no errors occurred.
Blinking green Battery level low.
Blinking red once
per second
Leakage detection. Pump turns off if end vacuum has not been
reached after 5 minutes.
Blinking red 5 times
per second
Vacuum generation is too fast (~100 mbar/s). Indicates a
clogged part.
Constantly red Battery error. Press the power button to continue using the
mains power supply.
Blinking red and green Error in vacuum sensor calibration. Please contact your supplier.

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4.3 Troubleshooting Problem Probable cause Remedy Device is not running. No electrical power. Check if mains adapter cable is plugged in or if battery
(model 159000) is discharged. Check indicator LED light.
Unit automatically switched off. Switch on the unit (LED lights up green).
VACUSIP automatically switches off if it is not used or 40 minutes after initial switch-on.
Aspiration speed is low. Leak in the vacuum system. Close the bottle lid tightly and attach tubing firmly. Check
tubing, filter and hand operator for leaks. If the maximum vacuum is no reached for 5 min, the pump is turned off.
Liquid overflowed and blocked the filter.
Empty bottle and exchange filter.
Tubing is blocked. Clean or exchange the tubing. Hand operator is clogged. Take apart the hand operator to clean it. Use an ultrasonic
bath to clean it of debris. Replace the hand operator.
VACUSIP turns off after a few seconds or minutes.
Clogging of a part close to the pump.
The LED blinks red rapidly. Check if filter is blocked and exchange it, if necessary. Check if the filter adapter is blocked.
Leak in the vacuum system. The LED blinks red slowly.
The pump automatically stops if the end vacuum is not reached after 5 min. Check for leaks in the system and push the power button again.

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5 Maintenance
5.1 Cleaning and servicing
WARNING
Always switch off the VACUSIP and disconnect it from the mains when carrying
out maintenance work.
Clean the VACUSIP housing with a lint-free cloth lightly soaked with mild soap solution in
distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never use bleach (sodium
hypochlorite) or other solvents.
It is recommended to change the hydrophobic filter regularly, at least once a year.
Exchange the filter immediately if liquid has entered.
When working with solvents and other hazardous materials always rinse the hand
operator and tubing with water followed by 70% Ethanol.
5.2 Autoclaving and chemical sterilization
5.2.1 Autoclaving
All parts contacting the liquid can be autoclaved: 500 ml glass bottle, lid with tube fittings,
silicone tubing and hand operator. The hand operator should be taken apart for optimal
decontamination. To disassemble it, hold the silicone cover and pull out the inner part.
Recommendation regarding autoclaving: glass bottle for at least 20 min at 121°C.
The bottle lid has to be always unscrewed and kept loosely on the bottle during
autoclaving.
Silicone may become brittle after extensive autoclaving. Replace the tubings if they are
damaged.
WARNING
The hand operator can be autoclaved sporadically for decontamination,
however it is not recommended to autoclave it regularly.
The filter and the instrument itself cannot be autoclaved.
5.2.2 Chemical sterilization
Two methods for chemical inactivation of biohazardous agents are commonly used:
• A suitable disinfectant is added to the full collection bottle and left to react for the
recommended time.
• The disinfectant is added to the empty bottle so that the aspirated liquid gets sterilized
as it accumulates in the bottle. Use disinfectants that are free of chlorine or other
corrosive agents, especially when corrosive vapors are generated and aspirated.

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5.3 Equipment disposal
The VACUSIP device must not be disposed of with unsorted municipal waste.
Do not dispose of the device in a fire.
VACUSIP (159000) contains a Li-ion battery. Do not modify the battery in any
way. Dispose of the VACUSIP device and the battery separately in accordance
with the laws and regulations in your area governing disposal of devices
containing Li-ion batteries.
6 Technical data
6.1 Specifications
6.2 Chemical compatibility
The table below lists VACUSIP parts that come into contact with the aspirated liquid or its
aerosols and vapors, and rates the compatibility of these parts to a few of the chemicals
commonly used in laboratories. To determine the compatibility of a component to a
chemical not listed in the table, please consult one of the several tables available on the
internet. Note that the rating refers to soaking in the concentrated chemical; however,
more relevant here is the attenuated effect resulting from vapors and the diluted chemical.
It is recommended to test the compatibility of relevant components to a specific chemical
prior to extensive use.
INTEGRA Biosciences does not warrant that the information in the table is accurate or
complete and that any material is suitable for any purpose.
Vacuum range –250 mbar +/- 20%
Flow rate (air) Pump: 1.5 l/min +/- 30% at ambient pressure
Dimensions
(H x W x D)
125 x 120 x 162 mm (base unit)
Height: unit with bottle and hand operator mount 345 mm
Weight Base unit only: 500 g
Unit complete: 970 g (with glass bottle, tubing and hand operator)
Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz
Device input: 8–10 VDC, 6 W
Battery rechargeable, Li-ion, ANSI 1604 size
Degree of protection IP 31
Ambient conditions Altitude: up to 2000 m
Temperature: 5 – 40 °C
Humidity: max. rel. humidity 80% for temperatures up to 31 °C
decreasing linearly to 50% rel. humidity at 40 °C.

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Chemical compatibility chart
Compatibility ratings: A = Good, no or minor effects. B = Fair, moderate effects, not recommended
for continuous use.
C = Critical, not recommended, suitability to be
determined by test.
JAVEL (e.g. NaClO)
Acetic acid AcOH
Ethanol EtOH
Isopropyl alcohol IPA
Sodium hydroxide
NaOH
Sodium acetate
(3M, pH 5.2)
Hydrochloric acid
HCl (20 %)
Nitric acid (20 %)
Dimethyl sulfoxide
DMSO
Phenol PhOH
Parts Materials Hand operator Inner part PP AAAAAAAAAA
Outer part Silicone ABAAACACAC Adapters POM CCAAAABCAA
INOX BCAABACAAA
Tubing Silicone ABAAACACAC Bottle Single-use PP AAAAAAAAAA
Lids PP AAAAAAAAAA Gasket EPDM AAAAAAAAAA
Filter Membrane PTFE AAAAAAAAAA
Housing PVC ACBAAAAACB
Filter adapter Silicone ABAAACACAC VACUSIP baseHousing PP AAAAAAAAAA
Pump housing POM C C A A A A B C A A Pump membraneNBR ABBAAAACBC

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7 Accessories and consumables
Accessories Part No.
Adapter 1-channel stainless steel adapter 40 mm 155 502
1-channel stainless steel adapter 150 mm 155 522
1-channel stainless steel adapter 280 mm 155 525
1-channel adapter for disposable tips/GripTips (pack of 5) 159 023
1-channel adapter with ejector for disposable tips 159 026
1-channel adapter with ejector for GripTips 159 027
4-channel stainless steel adapter 40 mm 155 524
8-channel stainless steel adapter 40 mm 155 503
8-channel adapter with ejector for disposable tips 159 024
8-channel adapter with ejector for GripTips 159 025
Stainless steel rod For holding the hand operator mount 159 045
VACUSIP hand
operator mount
To park the VACUSIP hand operator 159 046
Mains adapter
(100 - 240 VAC,
50/60 Hz)
EU 156 631
US 156 630
JP 156 634
UK 156 632
AU 156 633
Consumables Part No.
Filter For protection of the pump, non sterile, 0.45 µm 153 016
Tubing Silicone, 3 mm ID, 2.5 m 171 023
Silicone, 3 mm ID, 25 m bulk roll 171 033
Tubing set VACU-
SIP
Consists of: VACUSIP Hand operator,
Silicone tubing 1.2 m, Silicone tubing 0.22 m
1 filter (non-sterile, 0.45 µm)
159 040
Bottle INTEGRA borosilicate bottle with standard GL 45 blue
lid, 500 ml
159 031
Single-use PP bottle with GL 45 lid, 500 ml (pack of 10) 159 032
Green lid GL 45 with tube fittings (pack of 2) 159 035
Hand operator
VACUSIP
To operate VACUSIP and attach adapters 159 020
Battery Li-ion 155 066