Cole Parmer Stuart RE400 User manual

Rotary Evaporators
Instruction Manual
STU0002 / Version 1.0

2
Before use
If the equipment is not used in the manner described in this
manual the protection provided by the equipment may be
impaired.
Stuart rotary evaporators are designed to operate under the following
conditions:
❖ For indoor use only
❖ Use in a well ventilated area
❖ Ambient temperature range +5°C to +40°C
❖ Altitude up to 2000m
❖ Relative humidity not exceeding 80%
❖ Mains supply voltage fluctuations not
greater than ±10% of nominal
❖ Over voltage category 2 IEC 60364-4-443
❖ Pollution degree 2 IEC664
General description
Rotary evaporators are distillation units that incorporate an efficient
condenser with a rotary flask system. As the flask containing the
solvent is rotated it continually transfers a thin layer of liquid over the
entire inner surface. This gives a very large surface area for
evaporation that is effected by heating from the accessory waterbath.
Rotary Evaporators
RE400, RE401, RE402,
RE400P, RE401P, RE402P
Figure 1 Assembly
Location & Services
In order to get the optimum performance from your Stuart rotary
evaporator careful consideration should be given to the intended
location. Please study the following notes before commencing
assembly.
Choose a convenient location, which will allow easy access to both
rotating and receiving flask.
Assembly and operating instructions
The location should have access to the following services:
Cold water supply capable of delivering 60l/hr is required for RE400,
RE400P, RE401, RE401P.
Electricity supply
Drain
Vacuum line (optional)
Twist grip height adjustment handle 6
4
7
Rotating speed control
3
2
Vacuum and
water connections
On/Off switch
Glass angle control
5
1
9
Power supply holder

3
Electrical Supply
THIS EQUIPMENT MUST BE EARTHED
Before connection please read and understand this instruction
manual and ensure that the line supply corresponds to that
shown on the rating plate. Stuart rotary evaporators require a
supply rated at 100-240V, 50/60Hz, single phase,~.
Power consumption is:
Model
Power Frequency
RE400/P 8W 50/60Hz
RE401/P 8W 50/60Hz
RE402/P 8W 50/60Hz
IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN
IT IS IMPORTANT THAT THIS OPERATION SHOULD ONLY BE
UNDERTAKEN BY A QUALIFIED ELECTRICIAN
The wires in the mains cable are coloured as follows:
BROWN - LIVE
BLUE - NEUTRAL
GREEN/YELLOW - EARTH
The unit is fitted with an IEC socket at the rear of the instrument for
connection of the mains lead. The appropriate mains lead should be
connected BEFORE connection to the mains supply.
Should the mains lead need replacement a cable of 0.75mm2of
harmonised code H03VVH2-F connected to an IEC 320 plug should
be used.
Safety Advice before Use
❖If the jacking mechanism is to be raised without the glassware
fitted great care must be taken. Without the weight of the glass
the jack will rise rapidly and can cause damage to the
mechanism. Unlock the jack carefully while applying downward
pressure to control the movement.
❖As the apparatus is likely to be used under vacuum, before
operation, examine all glassware carefully for scratches or
chemical etching. Use of damaged glassware under vacuum
could result in an implosion. In any case it is recommended that
the apparatus be operated behind a safety screen.
❖DO NOT use the equipment in a hazardous atmosphere or for
mixing of hazardous materials.
❖Special care should be taken when using the equipment with
flammable solvents. The unit is not spark or explosion proof.
❖In case of mains interruption, a fault or mechanical failure, the
unit will continue to operate on removal of the fault.
❖Beware of safety issues associated with rotating glassware and
when jacking glassware up and down.
❖ DO NOT position the product so that it is difficult to access the
ON/OFF switch.
❖ DO NOT position the product so that it is difficult to disconnect it
from the mains supply using the mains plug.
❖ The mains outlet socket used should be located close to the
equipment and readily identifiable and accessible to users.
Assembly
If the jacking mechanism is to be raised without the
glassware fitted great care must be taken. Without the
weight of the glass the jack will rise rapidly and can cause
damage to the mechanism. Unlock the jack carefully while
applying downward pressure to control the movement.
1. Unpack the rotary evaporator and identify the following
components:
Item Component Catalogue
No Number
1 Condenser RE100/CO or RE200/VC or
RE200/CF and RE200/CFD
2 Conical joint clip KCM29
3 Feed/vacuum release adapter RE100/VR
4 Florentine flask 1L FD1L/4RE
5 Receiving flask 1L RE100/RF/1L
6 Rotary evaporator motor stand RE400/MS
7 Spherical joint clip JC35
8 Vapour tube RE100/VT or RE100/VT/CF
9 Grey retaining cap RE100/CS
10 Circular metal spring RE100/RS
11 Vacuum seal
12 Sub-seal RE100/VS
13 Retaining Clip
Figure 2
Figure 3
10
1
9
All components below are included with the
Rotary evaporator stand RE400/MS
The product is provided with a UK 3-pin for 230 V installations.
There are 2 power sockets on the rear of the stand, either of
which can be used. The spare socket should must not be used
with any other product, this is to avoid accident or malfunction.
- Power supply
- UK power supply lead
- Power supply holder
12
Bearing house
Flask
removal
sleeve
Lock Sleeve
13
8
Spring side
11

4
RE402, RE402P First remove the plastic connector at the back
of the condenser and fit into the hose. Secure with a suitable
clip, e.g. Jubilee. The connector and hose may now be fitted to
the vacuum line.
Note: A plastic cover is provided to reduce evaporation of cooling
agent.
Your rotary evaporator is now ready to be used.
Operation
2. Place the stand on a level surface. You will require easy access to
a water supply, drain, electric power and vacuum line to use your
rotary evaporator.
Use the base adjustment knob at the rear of the stand to adjust
the level of the unit. Turning the knob anti-clockwise will lower
the stand, turning the knob clockwise will raise the stand.
3. Pass the plain end of the vapour tube (8) through the right side
of the motor assembly and push into position until it locates
firmly behind the spring clip. The conical joint should be to the
right of the motor when viewed from the front.
4. Place the main vacuum seal (11) and sub-seal (12) onto the plain
end of the vapour tube (left) and slide them down until they
locate in the bearing housing. It is important that these
elements are fitted in the correct order and that the spring
loaded side of the main vacuum seal (11) faces the bearing
housing (figure 2).
5. Place the retaining clip (13) on the jointed side of the vapour
tube (right) and slide until it clips in the flask removal sleeve
(figure 2).
6. Place the grey retaining cap (9) over the butt joint of the
condenser (1) with the screwthread facing outwards and retain in
position with the circular spring (10) (figure 3).
7. Place the condenser over the vapour tube so that it butts up to
the vacuum seal. Ensure the spherical joint faces downwards.
Secure by screwing the retaining cap onto the bearing housing.
If using a RE400 diagonal coil condenser, check that the vapour
tube is central and will not foul the condenser cooling coil.
8. The condenser may now be set to the required angle:
RE400 and RE400P Diagonal position
RE401, RE401P and RE402, RE402P Vertical position
9. Support the condenser while loosening the glass angle control.
10. Position condenser and tighten the control.
11. Hold the receiving flask (5) in position and secure with the
spherical joint clip (7).
12. Place the rotating flask (4) onto the end of the vapour tube and
secure using the conical joint clip (2).
13. Remove the grey plastic screwcap from the narrow end of the
condenser and assemble it to the feed/vacuum release adapter (3)
(figure 4).
14. Pass the PTFE tube through the condenser and vapour tube so
that the end is inside the rotating flask. Note that for the RE301
and RE302, the PTFE tube may have to be reduced in length to
fit into the Florentine flask (figure 4).
15. Secure by tightening the screw cap.
16. RE402 and RE402P only: Assemble the drain stopcock (RE200/
CFD) to the condenser using the grey plastic cap located on the
lower side arm.
17. To connect the condenser to the water supply, drain and vacuum
line, flexible hose of 9mm I.D. is required. Ensure you identify
every connection correctly before starting. Note tubing suitable
for use under vacuum must be used for the vacuum connection.
RE400, RE400P First remove the plastic connectors from the
condenser and fit into the hose. Secure with a suitable clip, e.g.
Jubilee. The connectors and hose may now be fitted to the inlet
and outlet water connections on the condenser (bottom two
connections linked to the coil). Proceed in the same way for the
vacuum connection (top connection).
RE401, RE401P First remove the plastic connectors from the
condenser and fit into the hose. Secure with a suitable clip, e.g.
Jubilee. The connectors and hose may now be fitted to the inlet
and outlet water connections on the condenser (bottom two
connections linked to the coil). Proceed in the same way for the
vacuum connection (top connection).
RE400, RE400P, RE401, RE401P, RE402 and RE402P Control Panel
Anti-clockwise rotation mode is selected and the power outage
recovery mode is OFF when shipped from the factory.
While the unit is at normal stop status will appear on the display.
Rotation mode
Clockwise: The specimen flask turns clockwise.
Anti-clockwise: The specimen flask turns anti-clockwise.
Time: The speciman flask can be set to switch between turning
clockwise and anti-clockwise at a specified speed and for a specified
amount of time.
Setting the rotation direction and speed
1. Press the Power button. The display will come on and show the
number of rotations as .
2. Press and hold the Dial key for 5 seconds or more. The display
indicates and setting of the rotation mode is enabled.
3. Turn the Dial key to select the required rotation direction, the
selected rotation direction light will illuminate. Press the Dial key
once to enter chosen rotation mode.
4. Press the Dial key again. The display will flash or the number
or the number of rotations from the last session. The factory
setting is .
3. Turn the Dial key to the required rotation speed and press
the Dial key once to confirm. Turning the Dial key slowly increases
the number by one, turning the Dial key quickly increase by 10.
NOTE: You can change the rotation speed during rotation.
Power
Display
Clockwise
Dial key
Run / Stop
Time reversal
Anti-clockwise

Batch Operation
a) Remove the rotating flask (4) from the vapour tube (8) and fill
with the liquid to be evaporated. Ensure that the flask is never
more than half full. Secure the flask onto the conical joint using
the clip (2)
b) Examine the feed/release adapter (3) and ensure that both the
stopcock and vacuum release screwcap are closed (figure 4).
c) Ensure that water is flowing through the condenser at approx.
60l/hr (RE400 and RE401) or that the cold trap is filled with ice
or dry ice (RE402).
d) Switch on the electricity supply.
e) Raise the glassware assembly to its highest position by turning
the handle anticlockwise. Note that the mechanism is spring
loaded. Once at the right height, turn the handle clockwise to
secure the position.
f) Place a suitable water bath directly under the rotating flask.
N.B. A water bath designed for use with the RE400 is
available from Stuart, code RE400DB or RE400OB.
Please contact the sales office for details. 5
Setting the time reversal mode
1. Press the Power button. The display will come on and show the
number of rotations as .
2. Press and hold the Dial key for 5 seconds or more. The display
indicates , turn the Dial key to select Time. The Time and
Clockwise or Anti-clockwise lights will illuminate.
2. Press the Dial key once. The display will flash or the number
or the number of rotations from the last session. The factory
setting is .
3. Turn the Dial key to the required amount of rotations and press
the Dial key once to confirm. Then the display will alternate
between the timer indicator and the time used in the previous
session. The factory setting is .
4. While the display is flashing, turn the Dial key to the required
amount time. Press the Dial key once to exit Time reversal mode.
NOTE: The timer range is between 5 and 999 seconds.
NOTE: You can change the speed and time of rotations during
rotation.
Power outage recovery mode
Power outage recovery OFF: When the power is shut off during
rotation, rotation will stop when power is recovered. Display
alternates between number of rotations and . Display can be
cleared by pressing the dial key.
Power outage recovery ON: When the power is shut off during
rotation, rotation will resume when power is recovered. Display
alternates between number of rotations and . Display can be
cleared by pressing the dial key.
Setting the power outage recovery mode
1. Whilst is displayed in rotation mode, press the Dial key for 5
seconds or more. When the display indicates , turn the
Dial key to select or . Press the Dial key once to confrim
and exit power outage recovery.
g) Lower the glassware until the rotating flask is partially immersed
in the water.
N.B. Ensure that the glassware does not touch the bath
and that no water overflows as the flask is immersed.
h) Press the Power button. The display will come on and show the
number of rotations as .
i) Set the rotation speed to the desired speed ensuring that rotation
is not so fast that water is splashed out of the bath.
l) Press the Run / Stop button to begin
f) Press the Run / Stop button to begin
j) If evaporation at reduced pressure is required a vacuum line
should be attached to the vacuum side arm on the condenser.
Turn on the vacuum and set to required value.
N.B. A vacuum pump designed for use with the RE400 is
available from Stuart, code RE3022C. Please contact
the sales office for details.
k)
Set the temperature of the water bath as required.
m) When evaporation is complete release the vacuum by slowly
unscrewing the vacuum release screwcap (figure 4). Set the
rotation speed to 0, press the Run / Stop button to stop and raise
the glassware clear of the bath.
Continuous Feed Operation
When the apparatus is used under reduced pressure it is possible to
replenish the liquid in the rotating flask without interrupting the
evaporation or removing the flask.
The following procedure should be adopted.
a) Attach a length of flexible tubing to the continuous feed inlet of
the feed/release adapter (figure 4).
b) Carry out instructions b-j for batch operation.
c) Immerse the free end of the flexible tubing into the container
holding the liquid to be evaporated. Ensure there is no strain on
the feed/release adapter.
d) With the apparatus under vacuum slowly open the stopcock on
the feed/release adapter. The vacuum will draw liquid into the
rotating flask.
e) Close the stopcock when sufficient liquid has entered the flask
Further quantities of liquid may be added during evaporation by
reopening the stopcock.
N.B. Ensure there is sufficient capacity in the flask to cope
with the addition. Never fill the flask more than half full.
N.B. Ensure there is sufficient capacity in the receiving flask
to cope with the condensation.
N.B. This evaporator should not be used with rotating
flasks bigger than 2 litres or receiving flasks bigger than 1
litre capacity.
g)
If the receiving flask should require emptying during operation
the following procedure should be adopted:
Release the vacuum and stop rotation
Raise the glassware clear of the water bath
Wait until boiling stops and liquid stops dripping into the
receiving flask
The receiving flask may now be removed.
h) When evaporation is complete release the vacuum by slowly
unscrewing the vacuum release screwcap (figure 4). Set the
rotation speed to 0, press the Run / Stop button to stop and raise
the glassware clear of the bath.
i
ii
iii
iv
Continuous feed
stopcock
Continuous
feed inlet
Vacuum release
screwcap
Figure 4

Care and Maintenance
IMPORTANT: Before commencing any maintenance operation or
replacement of components parts the unit should be isolated
from the electricity supply, water supply and vacuum line.
These operations should only be undertaken by suitably
qualified personnel.
Glassware
All glassware parts should be cleaned regularly and examined for
scratches, cracks and chemical etching. Replace any damaged parts.
Plastic coated glassware
The plastic coated glassware should not be exposed to temperature
above 80°C and is not dishwasher proof.
Cleaning
The main stand and motor should be cleaned using a mild detergent
solution.
Vacuum Seal
It is recommended that the vacuum seal be regularly washed with
distilled or deionised water and allowed to dry naturally.
Removal of the Vacuum Seal
a) Remove the rotating and receiving flasks
b) Remove feed/release adapter
c) Remove condenser
d) Withdraw vapour tube from the bearing housing by completely
unscrewing the plastic screw bush on the right side of the bearing
house.
e) The vacuum seal may now be removed.
f) Reassemble following the assembly instructions in this manual.
Servicing
It is recommended that any servicing or repair is only undertaken by
suitably qualified personnel.
Only spare parts supplied by Cole-Parmer or its agent should be used.
Fitting of non-approved parts may affect the performance of the
safety features of the instrument.
If in doubt, contact the Technical Service Department of
Cole-Parmer Ltd.
Beacon Road,
Stone, Staffordshire,
ST15 0SA, United Kingdom
Tel: [phone]
Email: [e-mail]
Web: www.stuart-equipment.com
6
The following spare parts are available from your laboratory supplier.
(For a comprehensive parts list or circuit and wiring diagrams please
contact the Technical Service Department of Cole-Parmer quoting
model and serial number).
Cat. No. Description
RE100/CO Diagonal coil condenser (feed tube not included)
RE200/VC Vertical coil condenser (feed tube not included)
RE200/CF Cold finger condenser (feed tube and drain not included)
RE100/COP Diagonal coil condenser plastic coated (feed tube not included)
RE200/VCP Vertical coil condenser plastic coated (feed tube not included)
RE200/CFP Cold finger condenser plastic coated (feed tube and drain not included)
RE200/CFD Drain for cold finger condenser
RE100/VR Feed tube
RE100/VT Vapour tube for diagonal condenser
RE100/VT/CF Vapour tube for vertical coil and cold finger condensers
RE100/VS Vacuum seal
FD1L/4RE Florentine flask 1000ml
FD1L/4REP Florentine flask 1000ml plastic coated
RE100/RF/1L Receiving flask 1000ml
RE100/RF/1LP Receiving flask 1000ml plastic coated
KCM29 Conical joint clip
JC35 Spherical joint clip
Technical Specification
RE400/P RE401/P RE402/P
Speed, rpm 10 - 310
Vacuum 3mm Hg
Lift Distance 180mm
Overall dimension
(Highest position mm)
672w x 342d
x 504h (684mm)
479w x 342d
x 823h (1003mm)
514w x 342d
x 645h (825mm)
Electrical supply 100 - 240 V, 50/60Hz, 8W
Warranty
Cole-Parmer Ltd. warrants this mechanical and electrical equipment
to be free from defects in material and workmanship, when used
under normal laboratory conditions, for a period of three (3) years. In
the event of a justified claim Cole-Parmer will replace any defective
component free of charge.
This warranty does NOT apply if damage is caused by fire, accident,
misuse, neglect, incorrect adjustment or repair, damage by installation
adaptation, modification, fitting of non-approved parts or repair by
unauthorised personnel.
This warranty does not apply to the glassware or vacuum seal.
Spares

EU Declaration of Conformity
Product Laboratory Equipment
Manufacturer Cole-Parmer Ltd
Beacon Road
Stone, Staffordshire
ST15 0SA
United Kingdom
File Number P225
Authorised Cole-Parmer
Representative Beacon Road
Stone, Staffordshire
ST15 0SA
United Kingdom
Object of Declaration Rotary Evaporators - RE Range
(reference the attached list of catalogue numbers)
The object of the declaration described above is in conformity with the relevant Union Harmonisation Legislation:
References to the relevant harmonised standards used or referen
to which conformity is declared:
Additional Information
Place of Issue
Date of Issue
Authorised Representative
Title
Signature
This declaration of conformity is issued under the sole responsibility of the manufacturer
Signed for and on behalf of the above manufacturer
Low Voltage Directive
EMC Directive
RoHS Directive
2014/35/EU
2014/30/EU
2011/65/EC
Year of CE Marking: December 2016
Stone, Staffordshire, UK
07 July 2017
Steve Marriott
Technical Director
Electrical equipment for measurement, control and
laboratory use. EMC requirements.
Part 1: General requirements (Class A).
IEC/EN 61326-1:2013
IEC/EN 61010-1:2010
Safety requirements for electrical equipment for
measurement, control and laboratory use.
Part 1: General requirements.
Particular requirements for laboratory equipment for
mixing and stirring.
IEC/EN 61010-2-051:2015
s.
and so we cannot guarantee that interference will not
occur in practice. Where there is a possibility that injury,
damage or loss might occur if equipment malfunctions
due to radio frequency interference, or for general
advice before use, contact the manufacturer.
This product meets the applicable EC
harmonised standards for radio frequency
interference and may be expected not to
interfere with, or be affected by, other equipment with
similar qualifications. We cannot be sure that other
equipment used in its vicinity will meet these standards
Declaration of Conformity is also available to view online at www.stuart-equipment.com

Cole-Parmer Ltd.
Beacon Road,
Stone,
Staffordshire,
ST15 0SA,
United Kingdom
Tel: [phone]
Email: [e-mail]
Web: www.stuart-equipment.com