Contherm MITRE 4150 User manual

Scientific Ltd
SERVICE MANUAL
MITRE 4000 SERIES
Sterilising - Standard/Tropicool/Cooled Models
CO2CELL CULTURE INCUBATORS
CONTHERM SCIENTIFIC LIMITED
P O BOX 30-605 LOWER HUTT 5040
TEL: [phone]
FAX: [phone]
EMAIL: [e-mail]

WARRANTY STATEMENT
*******
CONTHERM Scientific Company will guarantee CONTHERM equipment for
aperiod of twelve months from the date of installation against faulty
workmanship and fabricated materials. This guarantee covers the
replacement of component parts found to be defective and authorised
labour charges during this period.
Should a malfunction occur or condition develop beyond reasonable
acceptance the company will accept responsibility for returning the unit to
its factory specification at no cost to the Purchaser providing that the
operating instructions have been observed and the defect is due solely to
faulty design, material and workmanship. That the defective part be
returned, freight paid to the nearest sales service office. Units outside the
warranty period will be accepted and repairs will be covered under an
extension of the above for 90 days.
In remote installations where it is not possible for the company's or
agents' engineers to attend, authority may be given to allow the Purchaser
to arrange such service.
The Purchaser is required to remit the purchase price of the unit to the
supplier within the terms of that supplier's condition of sale. CONTHERM
Scientific Company will accept no liability or shall its agents for
consequent damage of any kind due to a malfunction or component
failure.
*******

MANUALP/4000_SERVICE_0712 July2012 4
STATEMENT of CONFORMITY
This CONTHERM cabinet conforms to the following standards:
•Electrical Safety: Designed to NZS6200 / AS3100:1994
•EMC: Complies with AS/NZS 2064: 1997

MANUALP/4000_SERVICE_0712 July2012 5
CONTENTS
SECTION 1 Definition of Terms
SECTION 2 Introduction and Specifications
SECTION 3 Installation
SECTION 4 Operating Instructions
SECTION 5 Calibration
SECTION 6 Theory of Operation
SECTION 7 Customer Troubleshooting and Maintenance
SECTION 8 Spare Parts Lists
SECTION 9 Fault / Alarm Codes
SECTION 10 Servicing / Replacing Parts
IMPORTANT All electrical servicing must be carried out by
suitably qualified personnel only.

MANUALP/4000_SERVICE_0712 July2012 6
SECTION 1 DEFINITION OF TERMS
For the purpose of our standard specifications the following definitions shall
apply:
a) WORKING SPACE
That portion of the internal space which is above the lowest shelf and
not less than 5cm from any wall (including roof).
b) CHAMBER TEMPERATURE
That temperature at the centre of the working space.
c) SPATIAL VARIATION
The difference between the midrange of all measured temperatures
obtained at one site and that at another site for those sites which give the
greatest difference.
d) TEMPORAL VARIATION
The maximum value of the temperature range obtained for the standard
site with the greatest range throughout the test interval.
e) TEMPERATURE OVERSHOOT
Any excess of actual over desired chamber temperature during a
heating up period.
f) TEMPERATURE REPRODUCIBILITY
Temperature regained without alteration to controls.
g) TEST INTERVAL
Interval of time to which the steady state characteristics apply (Max 1
hour).
NB: All the above apply with an empty chamber.
For definitions and test methods refer: AS2853 : 1986 (and Appendices)

MANUALP/4000_SERVICE_0712 July2012 7
SECTION 2 INTRODUCTION AND SPECIFICATIONS
The CONTHERM CAT4000 Mitre Series of Co2 Culture Incubators has been
expressly designed to give the widest choice of operating conditions consistent
with high reliability and low cost, the addition of microprocessor control gives
additional versatility and resolution while improving the degree of temperature
control. These Incubators are fitted with a UVC (254nm) SERILISING UV lamp.
All specifications are quoted for an enclosure temperature of +37oCwith an
ambient Temperature of +20oCfor Standard and Tropicool cabinets and for an
enclosure temperature of +20oCfor cooled cabinets.
•Construction - Combination of High quality stainless steel and zinc plated
mild steel; epoxy coated exterior, full fibreglass insulation, High quality
stainless steel interior with full access door and magnetic door catch
•Safety - Fitted with an independent factory set Hi-Limit and user adjustable
Hi-Limit completely separate from normal controls.
•Convection - All units come with mechanical convection fan systems.
•Electrical - Designed to NZS6200 / AS3100:1994 240V AC M.E.N
•EMC - Complies with AS/NZS 2064: 1997
•All performances quoted at 20oC, ZP22 Microprocessor control System.
PERFORMANCE:
a)Temperature:
Nominal Range: Standard (Ambient +5oC) to +50oC
Tropicool (Ambient -5oC) to +50oC
*Cooled +15oCto +50oC
Temporal Variation Standard +_ 0.2oC
(Tropicool) +_ 0.3oC
(Cooled) +_ 0.4oC
Spatial Variation Standard +_ 0.4oC
(Tropicool) +_ 0.4oC
(Cooled) +_ 1.5oC
Initial Overshoot +2.0oC
Reproducibility +_0.5oC
Dial resolution 0.1oC
Operating Ambient 10oC - 35oC
Mains Voltage Range 230-250 AC 50Hz
*NB: Can only be operated on 60Hz when cooled units fitted with a special
refrigeration compressor.

MANUALP/4000_SERVICE_0712 July2012 8
NB: The lowest temperature performance is only achievable in a maximum
ambient of +20oC
NB: If fitted with a HEPA filter, the spatial specification is downgraded by a
further ±0.4oC
b) Relative Humidity -
With the standard tray of water inside a standard cabinet the humidity will
typically rise to 80% RH or more at a temperature of 37.0oC. The
maximum achievable humidity will be less in a Tropicool or Cooled
cabinet.
NB: On COOLED units the supplied stainless steel water tray should be placed
on the cabinet floor and pushed so that the sloping side firmly touches the rear
wall.
c) Carbon Dioxide:
Range: 0.5 - 20 % CO2
Fluctuation (@35Kpa / 5PSI) ±0.2% CO2
Accuracy: (@37oC, 85% RH) ±0.5% CO2
CAUTION: This chamber has been fitted with a SPECIAL UVC Sterilising lamp
located behind the stainless steel rear duct. Note that this ULTRA-VIOLET
lamp is HARMFUL to the eyes and MUST NOT be viewed directly or indirectly
for any period.
The lamp is automatically turned OFF when the outer door is opened and turns
on for 5 minutes when the outer door is reclosed.
If for ANY reason the glow from the lamp can be seen when the outer door is
opened, the cabinet must be turned OFF and the cause investigated by a
qualified service engineer.
SPECIAL NOTE for COOLED incubators
COOLED chambers may be fitted with a ‘FRIDGE’ switch located on the RHS
monitor panel. If fitted, this switch allows the user to turn the refrigeration
system OFF when operating at higher temperatures and low ambient condition
(for example at 37oCin an air-conditioned room).
If the ‘FRIDGE’ switch is turned ON or OFF when the chamber has stabilised at
temperature, the control temperature may be disturbed for a short period while
the chamber re-stabilises at the new load setting.
When operating at 37oCin an air-conditioned room, it is recommended to
set the ‘FRIDGE’ switch to OFF.

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SECTION 3 INSTALLATION
The CAT 4000 Mitre Series Incubators are designed to be installed into a
suitable well ventilated room with a preferred minimum clear space of at least
150mm on all sides to allow access for servicing. Mount the enclosure in its
final place ensuring that adequate space is allowed for the door to open. The
enclosure may be placed on the floor or a bench.
NB: On Tropicool and Cooled units ensure a space of at least 150mm is
allowed at the rear of the cabinet for air circulation – On Tropicool units ensure
that the unit can not be pushed too close to a rear wall. Locate the two stainless
perforated “butterfly wings” at the rear sides of the Tropicool unit and gently
bend to an angle of 90o.
The chamber requires a 240Volt 50HZ 10Amp Single phase EARTHED
electricity supply.
Total electrical load is upto 0.75Kw (4400C).
Maximum shelf loading is 30Kg/shelf, maximum total for cabinet is 100Kg.
The chamber should be thoroughly inspected for any signs of mechanical
damage that may have occurred in transit before any attempt is made to apply
power to the unit.
1) Connect a bottle of DRY FOOD GRADE CO2fitted with a two stage
Pressure Regulator set at 35kpa (5psi) to the Hosetail No 1 at the rear
of the enclosure.
2) If the enclosure is fitted with the automatic CO2CHANGEOVER option fit
the second gas bottle to Hosetail No 2. This bottle will automatically be
selected when bottle No 1 is empty.
3) Install the shelf guides in the desired positions. Slide the shelves into
guides.
4) On cooled / Tropicool units a large stainless steel or plastic tray is used to
both provide and collect water from the refrigeration system evaporator.
Ensure this tray is pushed firmly against the rear wall of the cabinet. If
elevated humidity conditions are desired partially fill the tray with distilled
or deionised water.
NOTE: The hosetail is a nominal ¼” brass barb. Typically use 6.0mm ID PVC
beverage tubing or similar to connect to the gas bottle regulator output. The
tubing should be secured to the hosetail using S/S hose clips.

MANUALP/4000_SERVICE_0712 July2012 10
WARNING: To prevent FIRE or SHOCK hazard, DO NOT expose this
product to rain or any type of moisture.
FOR YOUR SAFETY
To ensure safe operation the three-pin plug supplied must be inserted
ONLY into a standard three-pin power outlet which is effectively earthed
through the normal building wiring.
Extension cords are NOT recommended.
The fact that the equipment operates satisfactorily does NOT imply that
the power outlet is earthed and that the installation is completely safe. For
your safety, if in any doubt about the effective earthing of the power outlet,
consult a qualified electrician.
This appliance should be regularly tested (or after any service work on
the appliance) according to the procedures prescribed in AS/NZS 3760.
The basic safety checks and tests on electrical appliances required by
AS/NZS 3760 are:
a) A visual check to ensure that there is no mechanical damage, that
controls etc. are in good working order and that no parts are
missing.
b) An earth continuity test. (Maximum allowed resistance is 1 )
An insulation resistance test. (Minimum insulation resistance is
1 M)
WARNING: This cabinet is fitted with a UV Sterilising Lamp
UVC refers to shorter UV wavelengths, usually 200 to 280 nM. Wavelengths
in the UVC range, especially from the low 200's to about 275 nM, are
especially damaging to exposed cells. Such shortwave UV is often used for
germ killing purposes. The typical wavelength for ‘sterilising’ purposes is
253nM.
CAUTION: This chamber has been fitted with a SPECIAL UVC Sterilising lamp
located behind the stainless steel rear duct. Note that this ULTRA-VIOLET
lamp is HARMFUL to the eyes and MUST NOT be viewed directly or indirectly
for any period.
The UV lamp is automatically turned OFF when the outer door is opened and
turns on for 5 minutes when the door is reclosed.