CoolLED pE-300 Series User manual

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User Manual
pE-300 Series
White Light Fluorescence Illumination System

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Table of Contents
1. Introduction .................................................................................................................... 3
2. Safety Precautions........................................................................................................... 4
3. pE-300 Series variants..................................................................................................... 6
4. Getting Started –System Components........................................................................... 8
5. Installation and setup...................................................................................................... 9
6. Configuration of LEDs as a White Light Source............................................................. 11
7. Operation - Manual Control.......................................................................................... 13
8. Remote Operation –TTL (pE-300white & pE-300ultra)...................................................... 17
9. Remote Operation –USB (pE-300white & pE-300ultra)..................................................... 23
10. Optical setup ................................................................................................................. 25
11. Additional filtering (pE-300ultra)..................................................................................... 27
12. Settings / Additional Information ................................................................................. 29
13. Routine Care and Maintenance .................................................................................... 32
14. Fitting the pE-300 Series Illumination System to a different microscope .................... 33
15. Product specifications................................................................................................... 34
16. Product options and order codes.................................................................................. 35
17. Warranty and Repairs ................................................................................................... 35
18. Compliance and Environmental.................................................................................... 35
19. Contact Details.............................................................................................................. 36
20. Appendix 1 .................................................................................................................... 37

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1. Introduction
CoolLED’s pE-300 Series illumination systems are designed to offer broad
spectrum LED illumination for general use in fluorescence microscopy
applications. The pE-300 Series can be fitted directly to the microscope as a
better and safer alternative to high pressure mercury or metal halide
illuminators. Spectral coverage is from the UV (DAPI excitation) to the Red
region (Cy5 excitation). It will excite common fluorophores used in hospital
and research environments.
With a comprehensive range of microscope adaptors, the pE-300 Series can
be fitted to most current and older microscopes. The result is a safe,
convenient illumination system which will last for many years without any
additional operating costs.
This User Manual should give you all the information required to install and
operate your new illumination system.
Additional information can be found on our website at www.coolled.com

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2. Safety Precautions
While LEDs are a much safer illumination system than the mercury and
metal halide lamps that they replace in microscopy applications, precautions
should still be taken with this product.
When operating or maintaining this product, please observe the following
safety precautions at all times. Failure to do so may result in personal injury
or damage to other items.
Please ensure that only the power supply and cord supplied are used with
this equipment.
The AC cord supplied with this light source must only be used with the
equipment supplied.
2.1.
UV light may be emitted from this product depending on the
version/wavelength selected. Avoid eye and skin exposure. Never look
directly into the light output beam from the Light Source or accessories. The
emissions could damage the cornea and retina of the eye if the light is
observed directly.
2.2.
Always ensure that the Light Source is securely attached to the microscope
(either directly or with a light guide and collimator, depending on the
version) prior to turning on the power. This will minimise the risk of injury
and damage.
2.3.
If for any reason the Light Source is to be operated when not attached to a
microscope, all personnel should wear eye shielding and clothing to protect
the exposed skin.
2.4.
Disconnecting the mains supply is achieved by unplugging the power cord
from the power supply block or the Light Source. Only plug in the power
cable, once the Light Source is attached to the microscope.
2.5.
There are no serviceable parts within the Light Source. Removing any of the
screws and covers will result in the safety of the Light Source being impaired.
The DC power supply unit should be inspected periodically throughout the
lifetime of the system.

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2.6.
Any electronic equipment connected to this product must comply with the
requirements of EN/IEC 60950.
2.7.
To clean the exterior of the Light Source, use a slightly dampened cloth with
a simple water/detergent solution only. Avoid the optical surfaces and
lenses. Cleaning of optics should only be carried out using optical wipes and
fluids. Please note that the DC power supply unit should be isolated prior to
cleaning.
2.8.
This product conforms to the requirements of the Safety Standards as
follows:
EN/IEC 61010-1:2010 Safety Requirements for Electrical Equipment
for Measurement, Control and Laboratory use.
EN62471:2008 Photo-biological Safety of Lamps and Lamp
Systems/Guidance on manufacturing
requirements relating to non-laser optical
radiation safety. Risk Group 3.
2.9. EMC compliance
This product is tested to the requirements of standard IEC/EN 61326-1
concerning electromagnetic compatibility. This is a Class B product.
All warnings may not be applicable depending on the version/wavelength being used.

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3. pE-300 Series variants
All versions in the pE-300 Series offer intense, broad spectrum LED
illumination. With spectral coverage from the UV (DAPI excitation) to the
Red region (Cy5 excitation) they are suitable for imaging most common
fluorescent stains. Light sources are available for either direct attachment to
the microscope or via liquid light guide delivery.
3.1. pE-300white
The pE-300white allows individual control of the three channels in the
Illumination System. This can be controlled by the manual Control Pod, USB
or a single global TTL.
3.2. pE-300lite
The pE-300lite is the simplest Illumination System in the pE-300 Series. This is
controlled by the manual Control Pod, allowing global intensity control of
the illumination system.

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3.3. pE-300ultra
The pE-300ultra allows the greatest level of control in the pE-300 Series. The
Illumination System allows control of the three channels using the Manual
Control Pod, USB or by the four TTL inputs (one TTL input for each channel,
as well as a global TTL). One method of TTL control that is unique to the pE-
300ultra is the ability to control a multi-channel sequence using a single TTL
signal via the Sequence Runner feature. The pE-300ultra also allows additional
excitation filters to be placed in the light path of each of the three channels.

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4. Getting Started –System Components
A typical CoolLED pE-300 Series Illumination System is supplied with the
following components:
1. LED Light Source.
2. Manual Control Pod.
3. Microscope adaptor for specific microscope model (Direct fit only).
4. DC Power Supply Type GST120A12-R7B.
5. IEC Power Cable (not shown).
6. User Guide (not shown).
If any components are missing or appear damaged, please contact CoolLED
immediately.
The image shows a typical direct fit pE-300whitesystem.

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5. Installation and setup
5.1.
Carefully unpack the components from the shipping cartons.
5.2.
Insert the Control Pod cable into the LED Light Source using the red dots as a
guide for orientation of the plug.
5.3.
Connect the power connector from the DC power supply as shown. Ensure
that the DC power supply is the one supplied with the product. Using non-
CoolLED power supplies may damage the Light Source and will invalidate the
warranty. At this stage do not connect the mains power lead to the DC
power supply.

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5.4.
Attach the LED Light Source to the epi-fluorescence port on your
microscope. Your pE-300 Series Light Source will have been supplied with a
compatible fitting for the microscope you specified at order (if direct fit
version). Attach the Light Source ensuring that it is secure and true/flush
with the microscope.
5.5.
Ensure that there is free airflow around the LED Light Source so that the
cooling system is not impaired. A gap of 200 mm on either side is sufficient.
The diagram shows the Light Source in the preferred orientation. However,
it may be set with the cables at the top or at either side.
5.6.
With the LED Light Source now attached to the microscope it is safe to
connect the mains power. Connect the mains lead supplied to a convenient
socket, plug in the IEC connector into the DC power supply and switch the
power on at the socket.

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6. Configuration of LEDs as a White Light Source
6.1.
Conventional ‘white’ illumination systems used for fluorescence microscopy
(e.g. mercury lamps) have a single element which emits light in a series of
peaks across the spectrum, giving the effect of white light. LEDs are different
in that a single LED element will emit light in a particular colour. To create a
white illumination system, LEDs of different wavelengths have to be
combined together. Using a pumped phosphor, a broader peak covering
green, yellow and red emissions can also be created. In the pE-300 Series,
LEDs emitting in the UV and blue regions are combined with a pumped
phosphor to create a white illumination system covering all the commonly
used fluorescence stains.

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6.2.
The pE-300white and pE-300ultra have independent circuits giving the user
control of the three main peaks of emissions. On the standard configuration,
these are referred to as 1UV, 2B (blue) and 3GR (green, yellow, red).
6.3.
There is also a variant of the pE-300white and pE-300ultra which has been
configured for use with multi-band filter sets where the first peak has been
shifted from the UV region (1UV) to the violet (1V). See Appendix 1 for more
information.
6.4.
The pE-300lite also allows the choice between an SB and an MB configuration
to suit your filter sets. The Control Pod however displays only one ‘White’
intensity control bar on the display. This will allow global intensity control of
all installed LEDs at the same rate.

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7. Operation - Manual Control
7.1. pE-300white & pE-300ultra
7.1.1.
Manual Control Pod Operation on/off.
The pE-300white and pE-300ultra are easily controlled from the manual Control
Pod. LEDs are switched on and off by pressing the ‘on/off’ button.
7.1.2.
At start-up the Light Source will revert to the same settings that were set
when it was last powered down. New Light Sources are supplied with the
settings as shown.
7.1.3.
To switch on LEDs press ‘on/off’ once.

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7.1.4.
To switch off the LEDs, press the ‘on/off’ once again.
7.1.5.
Intensity Control.
The Control Pod enables the user to control the intensity of the LEDs that
are exciting different stains. This helps to balance the emissions so that one
stain does not dominate another. This feature is very useful in multi-band
work (see application note in Appendix 1).
7.1.6.
Reduce intensity of one channel by pressing the down intensity button.

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7.1.7.
Individual bands can be switched off (de-selected) by pressing the ‘select’
button. Light is then only generated where it is required to excite the stains
in use. This has many attractive benefits with improvements in contrast, cell
viability and savings in energy.
Switching off UV will help to reduce damage to cells through photo-
bleaching.
7.1.8.
Switch off selected channels by pressing the ‘on/off’ button.
7.1.9.
Switch selected channels back on by pressing ‘on/off’ button again.

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7.2. pE-300lite
7.2.1.
At start-up the Light Source will revert to the same settings that were set
when it was last powered down.
7.2.2.
To switch on the LEDs press the “on/off” button once.
7.2.3.
To control the intensity of the light output, use the “+” and “-“buttons to
increase or decrease in 1 % steps.

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8. Remote Operation –TTL (pE-300white &
pE-300ultra)
The pE-300white and the pE-300ultra can both be controlled remotely via a TTL
signal.
8.1. Global triggering (pE-300white & pE-300ultra)
8.1.1.
The pE-300white and the pE-300ultra both have a BNC socket on the rear of the
Light Source which allows global control of the Illumination System.
The TTL signal controls the on/off function of the Light Source. A TTL ‘high’
will cause the LEDs to be on, independent of the state of the on/off button.
Only those bands which have been manually selected on the Control Pod
(shown by a shaded intensity bar on the Control Pod display) will be
switched by the TTL signal. The intensities of the selected bands are
manually set on the Control Pod.
Global BNC
socket

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8.2. Individual channel triggering (pE-300ultra)
8.2.1.
In addition to the global TTL control available, the pE-300ultra also has three
additional BNC sockets that allow individual TTL channel control of the
Illumination System.
8.2.2.
The TTL signal controls the on/off function of the Light Source. A TTL ‘high’
will cause the LEDs to be on. The channel controls shall trigger the
corresponding channel regardless of its on/off state or whether it has been
selected using the Control Pod. The intensities of the selected bands are
manually set on the Control Pod.
8.3. Sequence Runner (pE-300ultra)
8.3.1.
The pE-300ultra allows the Illumination System to be controlled using the
Sequence Runner mode. The Sequence Runner allows sequential triggering
of multiple channels using a single TTL signal attached to the global BNC
socket.
Channel 1
Channel 2
Channel 3
Global TTL (used for
Sequence Runner mode)

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8.3.2.
The Sequence Runner mode is accessed by a short duration press of the
mode button on the Control Pod.
8.3.3.
ON/OFF state of the
Illumination System.
Installed channels
Order in triggering sequence
Channel intensity
(0-100 %)

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8.3.4.
Pressing the channel select button allows you to either deselect a channel or
change the order that it triggers in the sequence.
8.3.5.
Pressing the + & - buttons on the Control Pod will allow the light intensity of
the corresponding channel to be increased or decreased.