Cooper Surgical RI INTEGRA 3 User manual

User Manual
RESEARCH INSTRUMENTS LTD
Bickland Industrial Park, Falmouth, Cornwall, TR11 4TA, UK
Tel: [phone] | Email: [email protected] | origio.com
0120
Document 6-54-701UM, Issue 9, DRF 4525, 19 September 2018

CONTENTS
 
 
Intended Use 2
Applicable Part Numbers 3
Microscope Compability 3
Installaon 3
 
Guidance & Manufacturer’s Declaraon
- Electromagnec Emissions (IEC 60601-1-2) 5
- Electromagnec Immunity 6
Glossary of Safety/Informaon Symbols 8
 
System Components 10
Integra 3 System Component Part Numbers 11
Integra 3 System Microscope Adaptor Part Numbers 12
Hardware Overview 13
Device Label 14
Packaging and Handling Requirements 14
Power Supply Adapter 14
Integra 3 Specicaon Table 15
 
Petri Dish Posioning 17
Petri Dish Choice 17
Heated Stage Plate 18
Heated Metal Inserts 18
Heated Glass Inserts 18
Fing Micropipees 20
Inserng MPH into Toolholder 21
Inial Pipee Set-up Diagram 21
Inial Pipee Set-up Instrucons 21
Pipee Angle Adjustment 25
Roune Pipee Set-up 25
Shut Down Procedure 26
 
Display 27
User Interface Icons 28
Shortcut Buons 28
Stopwatch 29
Counter 29
Under-stage Lights 29
Height Indicators 30
Height Indicator Calibraon 30
System Informaon 31
 
Heated Stage Plate and Heated Metal Insert (Mode 1) 32
Heated Stage Plate and Heated Glass Insert (Mode 2) 32
Heated Stage Plate and
Heated Metal Insert with Thermosafe (Mode 3) 32
Heated Stage Plate Only (Modes 4 and 5) 33
Thermosafe 33
Seng the Temperature 34
Adjusng Specimen (Setpoint) Temperature 34
Temperature Calibraon 35
Calibrang Heated Plates 36
Calibrang Thermosafe Air Heang System 37
 
Status Indicator Light 38
Audible Alarms 38
Alarm System Tesng 39
Event Log 39
 
Safety 40
For ICSI in the USA 40
SAS - Screw-Actuated Air Syringe Assembly 41
SAS Specicaon Table 41
SAS Operaon 42
Notes on SAS 42
Equilibraon Procedure 43

Secon 1
Preface
1
1
Research Instruments Ltd
SOS - Screw-Actuated Oil Syringe Overview 44
SOS Specicaon Table 44
Fing Tubing to a SOS 45
Filling a SOS with Oil 46
Fing a Pipee 49
Using a SOS 49
 
Manipulaon 50
Temperature Controller 51
Alarm Code Tables 52
 
Manipulators 65
Replacing the Micropipee Holder Tip O-ring 66
Thermosafe Air Heang System 67
Air Syringes - Inserng a New O-ring 68
SAS 68
SAS-SE 68
Cleaning 69
Liquid Spills 69
Fuses 69
Disposal of Goods 69
 
Reuse Statement 70
RI Repairs System 70
RI Returns System 70
Contact Details 71
Obligaon to Inform 71
Feedback 71

Thank you for choosing the Integra 3.
This manual provides all necessary informaon to use the Research
Instruments Integra 3 micromanipulaon system. The system should be
operated by trained personnel only. All secons of this manual should be
read and understood fully before any operaon of the system. Please see
the Intended Use for more informaon.
If the operator is unsure of any of the informaon contained in this
manual, they should contact Research Instruments or an appointed
representave before aempng to use this equipment.
In no event does Research Instruments Ltd (RI) assume the liability for any
technical or editorial errors of commission, or omission; nor is Research
Instruments liable for direct, indirect, incidental, or consequenal
damages arising out of the use or inability to use this manual.
RI is constantly updang its products, and therefore reserves the right to
introduce changes in design, equipment and technical features at any me.
The Integra 3 manual belongs with the micromanipulaon system and
should be passed on with the Integra 3 micromanipulaon system if
relocated to another clinic.
The use of ™in this manual indicates a trademark of Research Instruments
Ltd. Any other brand names, referred to in this manual, are trademarks
of their respecve owners.
This indicates cauonary text which should be followed to avoid
injury to users or damage to samples.
The system should be operated by qualied and trained
personnel only.

Introducon to the Integra 3™

Research Instruments Ltd
2
Secon 2
Introducon to the Integra 3™
2
Research Instruments Ltd
2
Secon 2


Zeiss Axiovert 40/100/200, Axio Observer, Axio Vert.A1
Nikon TMD, Diaphot 200/300, TE200/300, TE2000, Ti
Leica DMIRB, DMI3000B/4000B/6000B, DMIL, DMi8
Olympus IMT2, IX50/70, IX51/71/81, IX53/73/83

Installaon of the Integra 3 system should be carried out by a Research
Instruments technician or other RI authorised personnel. Incorrect
installaon could result in poor pipee set-up and an overall poor
performance.
Relocaon of this system should be treated as a re-installaon and
should, therefore, be carried out by authorised personnel.
Please note that installaon and servicing is covered separately in the
Integra 3 Installaon and Service Manual (6-54-701IM). Users within the
USA will not be provided with Integra 3 Installaon and Service Manual.
The only user-serviceable parts are those detailed in the Troubleshoong
and Care and Maintenance Secons.
* TDU500 has now been replaced by current model TDU3.
Ensure that the mains power supply adaptor is prevented from
falling o the bench as this is likely to cause damage to equipment.
Applicable indicaons for use are subject to the regulaons of the country
into which the device is sold. Availability of an Integra 3 Micromanipulator
for clinical use is dependent on the regulatory approval status of the
Integra 3 Micromanipulator in the country the device is intended to be
sold into.


To precisely posion microtools under a microscope whilst maintaining
specimen temperature during Assisted Reproducon Techniques (ART)
such as Intracytoplasmic Sperm Injecon (ICSI) and Intracytoplasmic
Morphologically-selected Sperm Injecon (IMSI).
Contraindicaons: There are no contraindicaons associated with this
device.

The RI TDU500* Micromanipulator for Assisted Reproducon is
used to accurately posion microtools under a microscope for the
techniques of Assisted Reproducon.
The RI Integra is a conguraon comprising two TDU500*
micromanipulators, two air syringes and temperature control
system within a single housing.
: Federal law restricts this device to sale by or on the order
of a physician or a praconer trained and cered in its use.

0120
 
6-54-110 Integra 3 with Heated Metal Insert
6-54-100 Integra 3with Heated Metal Insert and Thermosafe

Integra 3™ Safety Warnings
5
Research Instruments Ltd
3
Secon 3
Integra 3™ Safety Warnings

Research Instruments Ltd
3
Secon 3
 

  

RF emissions
CISPR 11 Group 1
The Integra 3 uses RF energy only
for its internal funcon. Therefore,
its RF emissions are very low and are
not likely to cause any interference
in nearby electronic equipment.
RF emissions
CISPR 11 Class B The Integra 3 is suitable for use
in all establishments other than
domesc, and may be used in
domesc establishments and those
directly connected to the public low-
voltage power supply network that
supplies buildings used for domesc
purposes, provided the following
warning is heeded.
This equipment/system
is intended for use by healthcare
professionals only. This equipment/
system may cause radio interference
or may disrupt the operaon
of nearby equipment. It may
be necessary to take migaon
measures, such as re-orienng or
relocang the Integra 3 Or shielding
the locaon.
Harmonic emissions
IEC 61000-3-2 Class A
Voltage uctuaons/
icker emissions
IEC 61000-3-3
Complies
The Integra 3 is intended for use in the electromagnec environment
specied below.
The customer or the user of the Integra 3 should assure that it is used in
such an environment.
disassemble or modify any part of the Integra 3, or
substute any component for any other. Doing so may result
in damage to specimens, and this voids the warranty and/or
service contract.
 operate unless properly mounted to a microscope.
use the power cable and power supply adaptor supplied
with the system.
The cable to the power supply is the Disconnect Device for
this equipment. To remove all electrical power from this
product, disconnect the power cable from the electrical outlet.
Equipment should be posioned so as to allow easy access to
the power cable. The appliance coupler or mains plug is used as
the disconnect and must remain readily operable.
 To avoid the risk of electric shock, this equipment must
only be connected to a mains supply with protecve earth.
 Not to be used in a paent environment.
The microscope itself must be maintained to a high standard.
Problems such as worn focus mechanisms or an insecure video
camera may lead to unreliable focus and image stability, and
could lead to embryo damage.
 Do not modify this equipment without
authorisaon of the manufacturer.

Integra 3™ Safety Warnings

Research Instruments Ltd
3
Secon 3
Integra 3™ Safety Warnings

Research Instruments Ltd
3
Secon 3


This equipment is deigned to be used by professionals in a laboratory environment
and conforms to IEC 60601-1-2 electromagnec compability for professional use,
however it is recommended that Portable RF communicaons equipment (such as
mobile phones, hand held mobile radios etc) should not be used within 30cm of this
device as it could interfere with the performance of the Integra 3.

      
Electrostac
discharge (ESD)
IEC 61000-4-2
± 6 kV contact
± 8 kV air
± 6 kV contact
± 8 kV air
Floors should be wood, concrete or ceramic
le. If oors are covered with synthec
material, the relave humidity should be at
least 30 %.
Electrical fast
transient/burst
IEC 61000-4-4
± 2 kV for power
supply lines
± 1 kV for input/
output lines
± 2 kV for
power supply
lines
± 1 kV for input/
output Lines
Mains power quality should be that of a
typical commercial or hospital environment.
Surge
IEC 61000-4-5
± 1 kV line(s) to
line(s)
± 2 kV line(s) to
earth
± 1 kV
dierenal
mode
± 2 kV common
mode
Mains power quality should be that of a
typical commercial or hospital environment.
Voltage
dips, short
interrupons
and voltage
variaons on
power supply
input lines
IEC 61000-4-11
<5 % UT
(>95 % dip in UT)
for 0.5 cycle
40 % UT
(60 % dip in UT)
for 5 cycles
70 % UT
(30 % dip in UT)
for 25 cycles
<5 % UT
(>95 % dip in UT)
for 5s
<5 % UT
(>95 % dip in
UT) for 0.5 cycle
40 % UT
(60 % dip in UT)
for 5 cycles
70 % UT
(30 % dip in UT)
for 25 cycles
<5 % UT
(>95 % dip in
UT)
for 5s
Mains power quality should be that
of a typical commercial or hospital
environment. If the user of the Integra 3
requires connued operaon during mains
power interrupons, it is recommended
that the Integra 3 be powered from an
uninterrupble power supply or a baery.
Power
frequency
(50/60 Hz)
magnec eld
IEC 61000-4-8
3.0 A/m 50/60Hz 3.0 A/m
50/60Hz
Power frequency magnec elds should
be at levels characterisc of a typical
locaon in a typical commercial or hospital
environment.

      
Conducted RF
IEC 61000-4-6
Radiated RF
IEC 61000-4-3
3 Vrms
150 kHz to 80
MHz
3 V/m
80 MHz to 2.5
GHz
3 Vrms
3 V/m
Portable and mobile RF
communicaons equipment
should be used no closer to any
part of the Integra 3, including
cables, than the recommended
separaon distance calculated
from the equaon applicable to
the frequency of the transmier.


Note 1: At 80 MHz and 800 MHz, the higher frequency range applies.
Note 2: These guidelines may not apply in all situaons. Electromagnec propagaon
is aected by absorpon and reecon from structures, objects and people.
Note 3: These guidelines may not apply in all situaons. Electromagnec propagaon
is aected by absorpon and reecon from structures, objects and people.
a Field strengths from xed transmiers, such as base staons for radio (cellular/
cordless), telephones and land mobile radios, amateur radio, AM and FM radio
broadcast and TV broadcast cannot be predicted theorecally with accuracy. To assess
the electromagnec environment due to xed RF transmiers, an electromagnec site
survey should be considered. If the measured eld strength in the locaon in which
the Integra 3 is used exceeds the applicable RF compliance level above, the Integra 3
should be observed to verify normal operaon. If abnormal performance is observed,
addional measures may be necessary, such as re-orienng or relocang the Integra 3.
b Over the frequency range 150 kHz to 80 MHz, eld strengths should be less than
[V 1 ] V/m.
where p is the maximum output
power rang of the transmier
in was (W) according to the
transmier manufacturer
and d is the recommended
separaon distance in metres
(m). Field strengths from xed
RF transmiers, as determined
by an electromagnec site
survey a, should be less than
the compliance level in each
frequency rangeb.
Interference may occur in the
vicinity of equipment marked with
the following symbol:
80MHz to 800MHz
800MHz to 2.5GHz
d = [3.5/V1] √p
d = [3.5/V1] √p
d = [3.5/V1] √p
UT is the a.c. mains voltage prior to applicaon of the test level.

Secon 4
Integra 3™ Product Overview

Research Instruments Ltd
4
Integra 3™ Safety Warnings
8
Research Instruments Ltd
3
Secon 3

Welcome to the user manual for the Research Instruments Integra 3
micromanipulaon workstaon. This manual covers set-up, operaon
and maintenance of the workstaon. Please note that installaon and
servicing is covered separately in the Integra 3 Installaon and Service
Manual (6-54-701IM). Users within the USA will not be provided with
Integra 3 Installaon and Service Manual.
The Integra 3 provides a means of micromanipulang samples placed
under a microscope whilst maintaining sample temperature when
performing assisted reproducon techniques (ART).
An Integra 3 system consists of a baseplate which ts to a microscope, a
heated stage, two mechanical micromanipulators and two micropipee
holders. The system also includes syringes to apply sucon and pressure.
SAS air syringes are supplied as standard, but the SOS oil syringe or SAS-
SE ne control air syringe are available on request. Oponal components
include a double toolholder, an ITO heated glass insert and an air heang
system which is congured according to the customer’s microscope. All
heang systems are electrically powered.
Movement (micromanipulaon) is controlled by mechanical means
only with levers which provide both coarse (XY) and ne (XYZ) control.
Both temperature and movement are controlled manually by the user.

Source: ISO 15223-1:2016
BS EN 60601-1:2006 + A12:2014
 
In accordance with Annex I of Direcve 2014/35/EU for low
voltage and Annex 1 of Direcve 2014/30/EU for Electromagnec
compability.
Manufacturer
Date of manufacture
Serial number
Follow instrucons for use
Indicates the need for the user to consult the instrucons for use for
important cauonary informaon such as warnings and precauons
that cannot, for a variety of reasons, be presented on the device itself
Indicates instrucon for disposal of goods
Class 1 laser product (IEC60825-1:2014)
Direct current (DC)
This way up
Fragile, handle with care
Stacking limited to 3 units
Keep dry
0120
S
N
3



Secon 4
Integra 3™ Product Overview
11
Research Instruments Ltd
4
Secon 4
Integra 3™ Product Overview

Research Instruments Ltd
4
Figure 4-1

 
5-54-300 PL3, Le Hand
5-54-320 PL3, Right Hand
5-54-100 Baseplate Assembly
6-54-400 TDU3 (x2)
6-27-562 MPH (x2)
5-54-283 Heated Stage Insert (Heated Metal Insert, 16mm Diameter)*
5-54-290 Heated Stage Insert (Heated Metal Insert, 25mm Diameter)*
5-54-282 Heated Stage Insert (ITO Heated Glass Insert)*
2-00-893 12V 11A Medical Power Supply Adaptor
5-50-170 Handrest (x2)
6-34-520 SAS Air Syringe*
6-34-530 SAS-SE Air Syringe*
6-34-700 SOS Oil Syringe*
The following tables give part numbers of system components and
microscope adaptors. All parts are supplied or manufactured by Research
Instruments Ltd.

*Subject to order conguraon
Rear Adaptor
Heated Stage
Insert,
Baseplate,
TDU3,
Thermosafe
Handrest
SOS*
SAS-SE*
Front Adaptor
SAS*
MPHs
* Subject to order conguraon
Syringe Tubing
PL3
PL3
Power Supply
Adaptor

Secon 4
Integra 3™ Product Overview

Research Instruments Ltd
4
Secon 4
Integra 3™ Product Overview
12
Research Instruments Ltd
4 
5-54-120 Olympus IX51/71, IX53/73/83
5-54-140 Olympus IX50/70
5-54-134 Olympus IMT2
5-54-122 Nikon TE200/300, TE2000, Ti, Diaphot 200/300
5-54-136 Nikon TMD
5-54-142 Leica DMIL
5-54-124 Leica DMIRB/DMI3000B/4000B/6000B/DMi8
5-54-128 Zeiss Axiovert 100/200, Axio Observer, Axio Vert.A1
5-54-144 Zeiss Axiovert 40

 The front and rear adaptors are specied according to microscope.
Part numbers given below include the front adaptor, rear adaptor and
xing screws as required.
Please consult RI for tments to microscopes not listed above.
Figure 4-2

Figure 4-3
On/O
Switch
Thermosafe
Air Filter
(where ed)
Heated Stage
Insert
Coarse X,Y
Control Lever
Touch-screen
display
Fine X Y Z Control
Lever
Heated Stage
Plate
PL3 Toolholder
Shortcut
Buons
Stage Y Control
Stage X Control
Set-up Lever
Micropipee
Holder (MPH)
Baseplate

Secon 4
Integra 3™ Product Overview
15
Research Instruments Ltd
4
Secon 4
Integra 3™ Product Overview

Research Instruments Ltd
4
 
Manipulator
Fine Control
X, Y and Z movement from one lever
(X = side to side, Y = front to back, Z = up and down)
Sub-micron resoluon
0.75mm X and Y travel, 5mm Z travel
Manipulator
Coarse Control
X and Y movement
10 micron resoluon
4mm X and Y travel
PL3 Toolholders Pipee locaon
Pipee angle adjustment 16 to 43 degrees
Heang Systems
(conguraon dependent)
Heated Metal Insert: 16mm
25mm
ITO Heated Glass Insert
Thermosafe Air Heang System
Temperature Controller
Accuracy: beer than ±0.1°C when calibrated against
known reference.
Displayed resoluon: 0.1°C
Setpoint Temperature Range: 30-45°C
Displays 3.5” LCD touch screen display for temperature control
Connecvity
USB Type B socket for connecon to PC running RI Viewer
or RI Witness soware (where used). Refer to soware
manual for further informaon.
Connected PC to be compliant with IEC 60950-1
XY Stage Aluminium Heated Stage Plate
40mm travel in X and Y (25 and 33mm per turn)
Syringes Choice of SAS (air), SAS-SE (air) and SOS (oil)
Dimensions Footprint (not including microscope)
Width 56cm, depth 38cm
Weight
Maximum weight of microscope mounted components
(excluding front and rear adaptors): 10.4kg
Actual weight is conguraon dependent
Mains Power Adaptor Input: 100-240VAC, 50-60Hz, Max. 1.8A, Class I
Output: 12VDC, Min. 11A (132W)
Operang Condions
Temperature: 15°C (59°F) to 40°C (104°F)
Humidity: 15% to 85% RH (non condensing)
Pressure Range: 70kPa to 108kPa
Micropipee
Compability
Compable with Research Instruments micropipees
(or other industry standard 1mm OD micropipees of
equivalent specicaon).
Storage/Transport
Condions
Temperature: -40°C (-40°F) to 60°C (140°F)
Humidity: 15% to 85% RH (non condensing)
Pressure Range: 70kPa to 108kPa


To prevent transit damage to the equipment, the toolholders and, where
applicable, syringes are packed separately and must be ed before
operang the instrument.

Figure 4-4
use the power cable and power supply adaptor supplied
with the system.
Part No. 2-00-893
Power Supply Unit
Figure 4-5 Power supply unit label


Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5
Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5
The Coarse Control Lever moves the end of the micropipee through a
maximum of 4mm in the x-y plane for coarse posioning of the pipee.
The Fine Control Lever moves the pipee through a maximum of 0.75mm
in the x and y planes, and is used for accurate posioning of tools and
specimens for all normal procedures.
On each control the movement of the pipee exactly follows the
movement of the control lever. So, for example, moving a lever to the
le will move the pipee to the le.
The Fine Control Lever also provides Z movement of up to 5mm by the
rotaon of the lever.

Fine X Y Z Control
Lever Right
Fine X Y Z Control
Lever Le
Stage Y
Control
Stage X
Control
Coarse Control Lever Le Coarse Control
Lever Right
Figure 5-1
Status Indicator
Light
Switch on the power at the rear right hand side. The display will light up
and the indicated temperature of the heang systems will start to rise.
The system should not be used unl the Status Indicator Light shows a
connuously illuminated green light. This indicates that the temperature
has seled. Refer to the ‘Alarms and System Status’ secon for a full
denion of each Status Indicator Light colour.
The operator should be posioned in such a place to easily access the
microscope and also view the image on the PC monitor (where used).
Y
Z
X

Stage movement is controlled by the X and Y controls as described on the
previous page. The stage will move a maximum of 40mm in each direcon
ie 20mm from the centre posion. This allows viewing of a specimen
within the area of a Petri dish that is accessible to micropipees.
Figure 5-2
20mm20mm
20mm
20mm

For most applicaons, we recommend plasc Petri dishes that are
cered for use in ART clinics, which have a diameter of 50 to 60mm. A
dish with a low wall height may be preferable to allow the maximum area
of the dish to be accessible by the pipees.

Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5
Secon 5
Integra 3™ Basic Operaon
18
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5
Heated Glass Insert Frame
Do not place the Petri dish over the frame of the Heated Glass
Insert as this area is not heated (page 19).
Figure 5-3
Where an ITO Heated Glass Insert is used in conjuncon with a RI
Saturn 3, Saturn 5 or equivalent laser ablaon system, do not re
the laser with the microscope focussed on the ITO glass surface,
as this may damage the ITO coang.
The Heated Glass Insert is fragile and should be handled with
care. Do not apply excessive force to the glass surface.

Place the Petri dish in the centre of the insert, avoiding the frame of the
glass insert (gure 5-3). Short working distance objecves might touch
the insert before the specimen is brought into focus, depending on the
thickness of the glass and the dimensions of the Petri dish. Dishes with
a diameter larger than 60mm may need posioning by hand if the area
covered by specimen preparaons exceeds 40mm in diameter.

The moving stage plate is heated, allowing a dish to be posioned away
from the centre of the stage should this be required.

Place the Petri dish such that the area of interest is over the hole in the
centre of the insert. The eecve range of movement is limited by the
diameter of this hole (normally 25mm). Reposion the dish by hand to
view specimens that are outside the area of the hole. It may also be
necessary to reposion the dish if specimens are close to the edge of
the hole or if short working distance objecves are being used, as the
objecve might contact the insert.
Metal heated inserts are secured in posion using magnets, the insert
can be lied out by pulling upwards. When lowering the insert into
posion ensure that the insert is rotated unl the magnets re-aach the
insert securely.
The following sketch shows the locaon of the frame of the Heated Glass
Insert (the black area).
The Heated Glass Insert is secured in posion using magnets. The insert
can be removed by pushing gently from beneath. When lowering the
insert into posion ensure that the insert is rotated unl the magnets
re-aach the insert securely.
The RI Droplet Organisaon Guide (Part Number 6-30-600) can be used
to posion droplets within commonly used Petri dishes in order to ensure
that all droplets are accessible by the pipees.
Exercise cauon when reaching for stage XY control that the
Fine Control Lever is not knocked, especially when switching
to the Integra 3 aer using other stages that have the stage
XY control in a dierent posion.

Secon 5
Integra 3™ Basic Operaon
21
Research Instruments Ltd
5
Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5


The Integra 3 is designed to use industry standard 1.0mm diameter glass
micropipees, such as RI’s ICSI Plus range.
3. Insert the micropipee into the MPH p unl it passes through the
O-ring and hits the stop (inside the MPH).
1. Parally unscrew the Micropipee Holder (MPH) p.
MPH
30/08/12
REV
ASSEMBLY
DESIGN
DATE
DIMENSIONS IN mm
DRAWN BY
DATE
MATERIAL
TRACEABILITY
FINISH
SCALE
PRODUCT
TITLE
DRAWING
NUMBER
1:1
MPH assembly
AP
PURCHASING
SIGNATURES
THIRD ANGLE
PROJECTION
1
AX-MPH/1.0mm FOR PAR21
ECN SHEET
1
2
3
5
6
7
8
4
AA
B
B
CC
D
D
EE
F
F
TOLERANCES
(UNLESS
OTHERWISE
STATED)
X
0.5
X.X 0.1
X.XX
0.05
RESEARCH INSTRUMENTS LTD
GEOMETRIC 0.05
NO
BATCH
SERIAL
1 OF 1 911
Remove
2. Remove the micropipee from the Twista Pak.
Figure 5-4
Figure 5-5
Figure 5-6
4. Tighten the MPH p to clamp the micropipee in place.
Figure 5-7
MPH
30/08/12
REV
ASSEMBLY
DESIGN
DATE
DIMENSIONS IN mm
DRAWN BY
DATE
MATERIAL
TRACEABILITY
FINISH
SCALE
PRODUCT
TITLE
DRAWING
NUMBER
1:1
MPH assembly
AP
PURCHASING
SIGNATURES
THIRD ANGLE
PROJECTION
1
AX-MPH/1.0mm FOR PAR21
ECN SHEET
1
2
3
5
6
7
8
4
AA
B
B
CC
D
D
EE
F
F
TOLERANCES
(UNLESS
OTHERWISE
STATED)
X
0.5
X.X 0.1
X.XX
0.05
RESEARCH INSTRUMENTS LTD
GEOMETRIC 0.05
NO
BATCH
SERIAL
1 OF 1 911
MPH Tip
Requires the use of two 4x objecves
(one ed with a spacer)
Pipee
Angle Control
Raised Posion
Adjustment
Pipee Rotator
Set-up
Lever

Raise the Set-up Lever located on the side of the toolholder and rotate
the PL3 towards the front if desired. Place the narrow secon of the MPH
into the slot in the toolholder, then push inwards.
Figure 5-8
Lowered Posion
Adjustment
Centring
Control
Axial Drive
High pressure can be generated in the system when using small
diameter micropipees, viscous uids, or if a micropipee
becomes blocked. This may cause micropipees to shoot out of
the holder. Release the pressure before loosening the MPH p.
Rotate
toward
front
Micropipee
Holder (MPH)
MPH Tip
MPH
30/08/12
REV
ASSEMBLY
DESIGN
DATE
DIMENSIONS IN mm
DRAWN BY
DATE
MATERIAL
TRACEABILITY
FINISH
SCALE
PRODUCT
TITLE
DRAWING
NUMBER
1:1
MPH assembly
AP
PURCHASING
SIGNATURES
THIRD ANGLE
PROJECTION
1
AX-MPH/1.0mm FOR PAR21
ECN SHEET
1
2
3
5
6
7
8
4
AA
B
B
CC
D
D
EE
F
F
TOLERANCES
(UNLESS
OTHERWISE
STATED)
X
0.5
X.X 0.1
X.XX 0.05
RESEARCH INSTRUMENTS LTD
GEOMETRIC 0.05
NO
BATCH
SERIAL
1 OF 1 911
MPH
30/08/12
REV
ASSEMBLY
DESIGN
DATE
DIMENSIONS IN mm
DRAWN BY
DATE
MATERIAL
TRACEABILITY
FINISH
SCALE
PRODUCT
TITLE
DRAWING
NUMBER
1:1
MPH assembly
AP
PURCHASING
SIGNATURES
THIRD ANGLE
PROJECTION
1
AX-MPH/1.0mm FOR PAR21
ECN SHEET
1
2
3
5
6
7
8
4
AA
B
B
CC
D
D
EE
F
F
TOLERANCES
(UNLESS
OTHERWISE
STATED)
X
0.5
X.X 0.1
X.XX 0.05
RESEARCH INSTRUMENTS LTD
GEOMETRIC 0.05
NO
BATCH
SERIAL
1 OF 1 911
Insert

Do not place any force on the baseplate as this will aect the focus.
1. Move the Fine Control (red) and Coarse Control (blue striped) Levers
to a vercal posion. Rotate the Fine Control Lever to the centre of
the movement as indicated on the display (page 30). If the Height
Indicators have not previously been calibrated this must be done
rst as detailed on page 30.

Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5
Secon 5
Integra 3™ Basic Operaon
22
Research Instruments Ltd
5
Turn clockwise to lower,
an-clockwise to raise
2. Take an empty and clean Petri dish (set-up dish), and lightly scratch a
cross in its centre, on the upper surface. This must be the same type
of Petri dish as used clinically.
3. Select a low magnicaon objecve (4x without spacer). Place the
Petri dish on the stage and use the microscope focus control to focus
on the cross. Do not adjust the microscope focus again and do not
remove the dish unl the pipee set-up is complete.
4. Select the set-up objecve (4x with spacer).
5. Li the Set-up Levers to the raised posion. Insert holding and injecon
pipees into the MPHs and clip the MPHs into the toolholders (PL3).
6. Adjust the angle of each pipee by turning the Pipee Angle Control.
7. Align the bend in each pipee by turning the Pipee Rotator unl the
toe of the pipee is in a straight line.
8. Use the Axial Drive to move the pipees so that the ps are close to
each other. Look into the eyepieces and bring the p of each pipee
to the centre of the eld of view. Use the Axial Drive, if necessary, to
adjust the pipee p sideways. Use the Centring Control to move the
pipee p forwards or backwards. The p might be out of focus - this
does not maer and will be adjusted next.
9. Turn the Raised Posion Adjustment Screw on each side, not the
microscope focus, to bring the pipees into focus. Take care not to
apply too much downward pressure to the screw as this may cause
the PL3 to drop.
10. If the pipee cannot be brought into focus, remove the PL3 and
rotate the output post to raise or lower the PL3. If necessary, use a
tool in the holes in the post to assist in turning it. Each turn of the
post raises or lowers it by 0.5mm.

Secon 5
Integra 3™ Basic Operaon
25
Research Instruments Ltd
5
Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5Pipee Angle
Control
Pipee Rotator
Figure 5-9




Figure 5-10 Figure 5-11

Figure 5-12








Turn the Pipee Angle Control to increase or decrease the angle of the
pipee. The p of the pipee will stay in focus, so the angle can be
changed at any me during a procedure. For example, the pipee can be
set ‘toe down’ for sperm immobilisaon and then straight for injecon.
14mm

 Do not place any force on the baseplate as this will aect the
focus. If any step cannot be completed (for example, if the pipee p
is a long way out of focus at step 7), then carry out the inial pipee
set-up procedure as described on the previous pages.
11. Ret the PL3 and set the Raised Posion Adjustment Screw at the
centre of the range of movement using the PL3 Set-up Tool (or
measure a height of 14mm). Hold the tool as shown, just to the side
of the Raised Posion Adjustment Screw. Adjust the screw unl it just
touches at the top and boom of the square cut-out.
12. Repeat from Step 9. Move to Step 13 when the pipee can be brought
into focus with the Raised Posion Adjustment Screw.
13. Select the low magnicaon objecve without spacer. The scratch on
the Petri dish should sll be in focus.
14. Lower the Set-up Levers fully. The pipees should come into view
slightly above the Petri dish surface. If the pipee is too high or too
low, turn the Lowered Posion Adjustment Screw, 
to bring the p of the pipee just above the Petri dish surface.

Secon 6
Integra 3™ Electronic Control System

Research Instruments Ltd
6
Secon 5
Integra 3™ Basic Operaon

Research Instruments Ltd
5

1. Close RI Viewer soware (where used).
2. Switch o at rear.


When the Integra 3 is switched on, a white screen with the RI logo is
shown for a few seconds, then the main screen is shown. See User
Interface Icons on page 28 for an explanaon of all the icons.
 these show the actual temperature of each
heated area. Touch the icons to adjust the setpoint temperature of
each.
touch these to access sengs, calibraon and other
features.
see Height Indicators on page 30.
Temperature
Displays
Height
Indicators
Control Icons
1. Move the Fine (red) and Coarse (blue striped) Levers to a vercal
posion. Rotate the Fine Control Lever to the centre of the movement
as indicated on the display.
2. Take an empty and clean Petri dish (set-up dish), and lightly scratch a
cross in its centre, on the upper surface. This must be the same type
of Petri dish as used clinically.
3. Select a low magnicaon objecve. Place the Petri dish on the stage
and use the microscope focus control to focus on the cross. Do not
adjust the microscope focus again and do not remove the Petri dish
unl pipee set-up is complete.
4. Select the set-up objecve (4x with spacer).
5. Li the Set-up Levers (yellow striped) to the raised posion. Insert
holding and injecon pipees into the Micropipee Holders (MPH)
and clip the MPHs into the toolholders (PL3).
6. Adjust the angle of each pipee, if required, by turning the Pipee
Angle Control.
7. Look into the eyepieces and bring the p of each pipee to the
centre of the eld of view by using the Axial Drive Control (black). If
the p is out of focus, rotate the Fine Control Lever slightly (not the
microscope focus) to bring it into focus.
8. Align the foot of the pipee by turning the rotator on the end of
the MPH. It is horizontal when the pipee appears straight and
horizontal in the eyepieces.
9. Select the low magnicaon objecve without spacer. The scratch
on the Petri dish should sll be in focus.
10. Remove the set-up dish and replace with your clinical dish. Lower the
Set-up Levers fully. The pipee will come into view slightly above the
Petri dish surface. Small height adjustments can be made by rotang
the Fine Control Lever. If the pipees are consistently too high or too
low then carry out the inial pipee set-up procedure.

Secon 6
Integra 3™ Electronic Control System

Research Instruments Ltd
6
Secon 6
Integra 3™ Electronic Control System
28
Research Instruments Ltd
6
Start Video Recording**
Capture Sll Image**
Increment Counter

**Requires RI Viewer or RI Witness Soware
(oponal), see the User Manual supplied with
your soware.
Figure 6-1
+1

The following table explains the meaning of all the user interface icons
required to use and adjust the Integra 3 sengs and features. The icons are
referred to throughout this manual by their ‘Meaning’ in typeface.
eg is referred to as the  icon.
Icon  Icon 
 
 
 
 No
 
 
 
 
 
 
 
 
 
 
 
Please refer to the User Interface Icons table on the previous page for
images of the icons discussed below. The icons are idenable in the
following text by their  typeface.

The stopwatch can be accessed from the main screen by pressing the
icon. Once in the stopwatch screen the mer can be started
and stopped by pressing the and  icons respecvely, and can be
used between 0 and 59 minutes 59 seconds. Aer this me it connues
ming from 0 seconds. The icon can be used to zero the me. Press
the icon to return to the main screen.

The counter can be accessed from the main screen by pressing the
icon or the +1 buon on the le hand side of the Integra 3. The
counter can be used to count incrementally either upwards or downwards
between 0 and 99. To count upwards, press either the +1 buon or press
the icon on the touch screen. To count downwards, press the
 icon on the touch screen. To reset the counter, press the 
icon on the touch screen. Press the icon to return to the main screen.

In order to improve visibility underneath the Integra 3, the device is ed
with white LED lights. These are acvated by movement beneath the
Integra 3. The length of me the lights remain on aer detecng moon
can be adjusted as follows.
Press the icon and then the icon. The me
period can be adjusted between 0 and 9 seconds by pressing the 
and icons before pressing to exit. When set to 0 seconds
the lights will remain o. Press the icon to return to the main screen.
+1
?
* Please note that the Service Menu should only be accessed by service personnel.

Secon 6
Integra 3™ Electronic Control System

Research Instruments Ltd
6
Secon 6
Integra 3™ Electronic Control System

Research Instruments Ltd
6

These show the vercal posion of the toolholders, as set by rotang the
Fine Control Levers. If the Fine Control is turned close to the ends of the
range of movement, the display will turn red and the Integra 3 will emit
three short beeps, repeang every 5 seconds.
When commencing a procedure the TDU vercal posion should be
close to the centre of their range of movement. If the TDU needs to be
set away from the centre point in order to bring the pipee into focus
then follow the Inial Pipee Set-up Instrucons on page 21 before
proceeding.

The Height Indicators are factory calibrated. However, they can be
recalibrated as required. If resistance is felt when turning the Fine Control
Lever before the Height Indicator shows that the limit of movement has
been reached then re-calibraon is required.
Touch the  and then the  icon.
Avoid using the system in the posion pictured below, as there
may be insucient movement to complete the procedure.
For each side in turn, rotate the Fine Control Lever fully to the boom
of the range of movement. Touch the  icon. Rotate
the Fine Control to the top of the range of movement and touch the 
. Next rotate the Fine Control Lever 11 complete
turns downwards. It may help to put a pencil mark or small piece of
tape on the lever to count the turns. Now touch the 
icon. Touch the  icon to save the calibraon, then the icon to
return to the main screen.  If the  icon is touched without
saving then any changes to the calibraon will not be saved and it will
revert to the previous calibraon.

In order to provide addional informaon in the event of a fault, the
System Informaon screen can be accessed from main screen by
pressing the  and then the  icon. This screen is for
informaon only and it is not possible to change any values from this
screen. Press the icon to return to the main screen.
The following abbreviaons are used to idenfy parameters given on the
System Informaon screen:
   
 Instantaneous Temperature (°C)  Air Flow
 Temperature Setpoint (°C)  Air Flow Setpoint
 Calibraon Value (°C) SN Serial Number
 Heang Power (%)  Firmware Version
 Fan Speed (%)  Hardware Version
TDU

Secon 7
Integra 3™ Temperature Control

Research Instruments Ltd
7
Secon 7
Integra 3™ Temperature Control

Research Instruments Ltd
7
When Integra 3 is powered up, the display briey shows RI and the
product logo. Aer start up the screen shows the actual temperatures
of the devices connected. The heang systems are connually
monitored for safe operaon.
Various heang opons are available for the Integra 3.
A brief descripon and how to idenfy each opon is detailed below.
Refer to the Integra 3 Installaon and Service Manual (6-54-701IM) for
further informaon about changing the heang opon (mode).
To ensure embryo safety, the temperature setpoint should
be set to maintain the specimen at the correct temperature.
See ‘Adjusng Specimen Temperature’.
In the event of a temperature sensor malfuncon, the setpoint
temperature may be exceeded and the surface may become hot
to the touch. It is recommended that temperatures are checked
on a regular basis.

The Thermosafe Air Heang System passes a steady stream of warm air
across the central hole of a Heated Metal Insert. Air passes through a
lter, fan and heater before exing from the nozzle located close to the
central hole of the Heated Metal Insert.
 It is normal for the air heater enclosure to become warm when in
use.
The temperature of air exing the nozzle is controlled to a high degree of
accuracy, with the quanty of air preset to an opmum value to give the
most consistent heang across the central hole of the metal insert. As
with most microscope based heang systems, sample temperature will
be aected slightly by ambient condions.
The system has been designed to not interfere with the normal operaon
of the manipulaon system. Consequently, all components of this system
are located underneath the baseplate, with the excepon of the lter
which is located at the rear of the baseplate for ease of access.
Refer to the Care and Maintenance secon for requirements to change
the Thermosafe Air Heang System lter (Figure 11-2 on page 67).
The Thermosafe Air Heang System has been designed to
minimise audible noise and vibraon, which should not be
noceable under normal operang condions. In the event of
audible noise or vibraon from the system, contact RI for support.


The Heated Stage Plate and, when ed, the Heated Metal Insert
are shown on the main screen. The setpoint of each can be changed
individually, however, in pracce the setpoint of each will normally be
set to a similar value.

The Heated Stage Plate and when ed, the Heated Glass Insert
are shown on the main screen. The setpoint of each can be changed
individually. In pracce the setpoint temperature of the Heated Glass
Insert will normally be set slightly higher than the Heated Metal Stage.


The Heated Stage Plate and Heated Metal Insert can be used with the
Thermosafe Air Heang System. The setpoint of each can be changed
individually, however in pracce the setpoint of each will normally be
set to a similar value.

The Integra 3 can be congured to operate only the Heated Stage Plate.
This is typically only required when using the Integra 3 with an externally
controlled stage insert not manufactured by RI.
When using an externally controlled heang stage insert Mode 4 enables
the Heated Stage Plate setpoint temperature to be adjusted between 30
and 45°C.
When using an externally controlled heang/cooling stage insert, Mode
5 enables the Heated Stage Plate setpoint temperature to be adjusted
between 30 and 45°C or switched o.

Secon 7
Integra 3™ Temperature Control

Research Instruments Ltd
7
Secon 7
Integra 3™ Temperature Control

Research Instruments Ltd
7

The setpoint temperature of each heang system can be modied by
pressing the icons menoned on page 32, which are located on the
main screen. The value is modied by pressing the  or 
icons. Once the correct value is obtained, press the  icon to return
to the main screen.
When using the Heated Stage Plate only with an externally controlled
heang/cooling insert (Mode 5), an addional opon on the setpoint
screen enables the Heated Stage Plate to be switched o. In this case the
display will show ‘HEATING OFF’ when on the main screen.
It may take up to 20 minutes for the temperature to stabilise. In all
cases an alarm will sound if the setpoint temperatures are not reached.
Refer to the Alarm and System Status secon for a full descripon of alarms.

The heang systems are factory calibrated such that when the controller
is set to 37°C the surface temperature of the heated plate(s) and air
expelled from the Air Heang System (if ed) measures 37°C. For all
heang systems the setpoint temperature is adjustable to between 30°C
and 45°C.
The temperature inside a Petri dish will normally be slightly lower than
the heated surface, depending on ambient condions, type of Petri
dish and the sample preparaon. Aer the system has been installed
in its operang locaon, the temperature of the heated plate(s) and
Thermosafe Air Heang System (if ed) should be adjusted to allow for
this dierence.
Change the setpoint if the temperature in the Petri dish needs to be
changed. For example, the heated plate might need to be set to 41°C in
order for the specimen to be at 37°C.
Prepare a Petri dish with water and paran oil that mimics your normal
Petri dish preparaon and place it on the heated stage in its normal
posion.
Do not adjust the temperature with a specimen in place on the
heated surface.
Probe Posioned in Droplet Centred
over Objecve/Hole in Heated Plate
Probe Posioned in
Droplet over Heated Plate
Where the Thermosafe Air Heang System is ed, it is recommended
that temperature measurements are made above the centre of the
objecve over the heated plate (see Figure 7-1 on page 35).
For opmum temperature control across the hole in the heated plate,
adjust the setpoint temperature of the Thermosafe Air Heang System.
To control the area directly above the heated plate, adjust the heated
plate setpoint temperature. As a starng point, use the same setpoint
temperature for both heang systems.
In all cases, it is recommended that the objecve normally used for
manipulaon is in posion when measuring specimen temperature and
adjusng setpoint temperatures.
Figure 7-1

Perform calibraon only if the displayed temperature is dierent to the
actual temperature of the heated plate surface or the air expelled from the
air heater nozzle. The process of calibraon allows the user to manually
adjust the temperature so that the displayed temperatures match the
temperature of the heated plate or air expelled from the air heater nozzle.
We recommend using a thermometer calibrated to 37°C ed with a
small thermocouple probe, such as the RI IVF Thermometer. Place the
probe of the thermometer in the centre of the water drop and allow the
temperature reading to stabilise. Adjust the setpoint temperature unl
the desired temperature in the dish is reached, allowing 20 minutes (or
as long as required) in between each setpoint change to allow the Petri
dish temperatures to stabilise.