LC Packings Probot 161403 User manual

D960R3
Probot TM
Micro Fraction Collector
User’s Manual
P/N 161403
www.lcpackings.com

ii D960R3 User’s Manual Probot
Notice: The ProbotMicro Fraction Collector is covered by a limited warranty. A
copy of this warranty is included with this manual. The customer is required to
perform routine maintenance as described in the User’s Manual on a periodic
basis to keep the warranty in effect.
All information in this manual is subject to change without notice and does not
represent a commitment on the part of LC Packings, BV.
The material included in this manual is provided to assist users in the operation,
maintenance and repair of the Probot Micro Fraction Collector. It is assumed that
the individual using this manual has sufficient training in the use of analytical
instrumentation and is aware of the potential hazards including (but not limited
to) electrical hazards, chemical solvent hazards and the exposure to pressurized
solvents.
© November 2004, LC Packings, BV - A Dionex Company. All rights reserved.
No part of this manual may be reproduced or transmitted in any form or by any
means without the written permission of LC Packings, BV.
The following are Trademarks or Registered Trademarks of LC Packings - A
Dionex Company: Probot, Carrier, UltiMate, UltiChrom, FAMOS, Thermos,
Switchos II, Acurate, UZ-View, UV-Booster, FluoBoost, Pepmap, µ-Dumper, µ-
Fluidics, Fusica, Nano Series, Pico Series, µ-Guard, µ-Precolumn, Nano-
Precolumn.
Dionex™ is a Trademark of Dionex Corporation.
CHROMELEONis a Trademark of the Dionex Corporation.
PEEK™ polymer is a Trademark of Victrex plc.
Teflon® is a Registered Trademark of E.I. duPont de Nemours and Company.
Analyst® is a Registered Trademark of Applied Biosystems/MDS Sciex.
HyStaris a Trademark of Bruker-Daltonics Group.
MassLynx is a Trademark of Waters® Corporation.
Xcalibur® is a Registered Trademark of ThermoFinnigan.
BelkinTM and VideoBusIIare Trademarks of the Belkin Corporation.
Windows® and Microsoft® are Registered Trademarks of Microsoft Corporation.
Other product names or company names mentioned herein may be the
trademarks of their respective owners.
Printed in the Netherlands.

User’s Manual Probot D960R3 iii
Table of Contents
Warranty vii
Liability viii
Instructions for Returning Instruments ix
Warnings xi
Safety Precautions xi
CHAPTER 1 Introduction 1-1
1.1 Features of the ProbotMicro Fraction Collector 1-1
1.2 General Design of the Probot Micro Fraction Collector 1-2
1.3 Front View of Probot 1-4
1.4 The Manual Control Unit 1-4
1.5 Rear View of Probot 1-5
1.6 Control of the Probot Micro Fraction Collector 1-5
1.7 About this Manual 1-6
CHAPTER 2 Installation and Getting Started 2-1
2.1 Installation 2-1
2.1.1 Location of Probot in the Laboratory 2-2
2.2 Unpacking 2-3
2.3 Removing Shipping Components 2-3
2.3.1 Removing the Top Cover 2-4
2.3.2 Drive Table Safety Brackets 2-5
2.4 Installing the Probot with the UltiMate Capillary HPLC System 2-6
2.4.1 Electrical Connections 2-6
2.4.1 A RS-232 Connector 2-6
2.4.1 B START Connector 2-6
2.4.1 C REMOTE Connector 2-7
2.4.1 D TTL IN Connector 2-7
2.4.1 E INTERLOCK Connector 2-7
2.4.1 F RELAY Connector 2-7
2.4.1 G ANALOG IN Connector 2-8
2.4.1 H VIDEO Connector 2-8
2.4.1 I POWER Connector 2-8
2.4.2 Fluidic Connections for Fraction Collection 2-9
2.4.2 A Connecting the µ-Tee for Matrix Addition 2-9
2.4.2 B Connecting the Dosage Module 2-10
2.5 Software Installation 2-11
2.5.1 System requirements 2-11
2.5.2 Installation of Carrier 2-11
2.6 Introduction to the CarrierSoftware 2-12
2.6.1 Key Features 2-12
2.6.2 Design of µCarrier 2-13
2.6.3 The Editors and Wizards 2-13

Table of Contents
iv D960R3 User’s Manual Probot
2.6.4 Necessary Steps to create and run the Application 2-13
2.6.5 The Main Toolbar 2-14
2.7 µCarrier – First Steps 2-15
2.7.1 Launching µCarrier 2-15
2.7.2 Using the Predefined Applications, Trays and Sample Carriers 2-17
2.7.2 A Loading a Predefined Application 2-17
2.7.3 Alignment of the Sample Carriers 2-19
2.7.4 Checking and Modifying Application Parameters 2-21
2.7.4 A Checking and Modifying Groups 2-21
2.7.4 B Checking/Modifying the Tasks List 2-22
2.7.4 C Checking/Modifying the Task Parameters 2-23
2.7.5 Running a predefined Application 2-24
2.8 µCarrier – Basics Steps 2-25
2.8.1 Create a New Sample Carrier using the Sample Carrier Wizard 2-26
2.8.2 Create a New Tray using the Tray Setup Wizard 2-30
2.8.3 Modify an Existing Tray Setup using the Tray Editor 2-32
2.8.4 Create a New Application using the Wizard 2-34
2.8.5 Control the Table Position Manually 2-44
2.8.5 A Using the Manual Drive Panel 2-44
2.8.5 B Using the Manual Control Unit 2-44
2.8.6 Measure the Column (X) and Row (Y) Distance 2-45
2.8.7 Default Calibration and Acceleration Settings 2-47
CHAPTER 3 Maintenance and Troubleshooting 3-1
3.1 Overview 3-1
3.2 Maintenance 3-2
3.3 Replacing Major Components 3-3
3.3.1 Installing the Pipetting Option 3-3
3.3.2 Installing the Video Camera 3-4
3.3.3 Replacing the Syringe 3-4
3.3.4 Replacing the Dosage Unit 3-5
3.3.5 Replacing the Fractionation Needle of the µ-Tee 3-6
3.3.6 Replacing the Standard Needle 3-8
3.3.7 Replacing the Light Bulb of the Pen Light 3-8
3.3.8 Replacing the Main Fuse 3-9
3.4 Troubleshooting 3-10
3.5 Spare Parts Lists 3-11
3.5.1 Major Items 3-11
3.5.2 Accessories 3-11
3.5.3 Spare Parts 3-12
3.5.4 Adapter Tables 3-13
CHAPTER 4 Specifications 4-1
4.1 Physical 4-1
4.2 Electrical 4-1
4.3 Electrical Connections 4-2
4.4 Rotary Valve 4-2
4.5 Safety Devices 4-2

Table of Contents
User’s Manual Probot D960R3 v
APPENDIX A Dosage Module Purge Routine 1
A.1 Overview 1
A.1.1 The Step-By-Step Purge Routine 1
APPENDIX B Peak Collection in Conjunction with CHROMELEON®
Software 1
B.1 Overview 1
B.2 Contact Closure Signals for Peak Detection with CHROMELEON 2
B.3 Setting up µ-Carrier for Peak Collection 3
B.4 Electrical Connections 6
B.5 Listing of the CHROMELEON Program used for this Example 6
B.6 Additional Hints/Troubleshooting 8
APPENDIX C Table Alignment Instructions 1
C.1 Overview 1
C.2 Table Alignment 2
C.2.1 Leveling 2
C.2.2 Test Needle and Carrier Setup 2
C.2.3 Checking and Aligning of the X-axis and Y-axis 3
C.2.4 Alignment of the X-axis 4
C.2.5 Alignment of the Y-axis 4
APPENDIX D The Vacuum Waste Removal Option 1
D.1 Overview 1
D.2 General Design 2
D.3 Setting up the Probot 3
D.3.1 Electrical Connections 3
D.3.2 Connecting the Vacuum Pump 3
D.3.3 Installing the Fused Silica Capillary 4
D.4 Setting up µCarrier and CHROMELEON5
D.4.1 Enabling the Vacuum Waste Removal Option 5
D.4.2 Adjusting the Fused Silica Capillary and the Needle Height 5
D.4.3 Using the Waste Removal Option - Example 6
D.5 Listing of the CHROMELEON Example Program 8
D.6 Additional Hints/Troubleshooting 9
APPENDIX E Installing and Using the Belkin
 USB Video Adapter 1
E.1 Installation of the BelkinVideoBusIIImage Capture Device 1
E.2 Software Installation and µCarrier Setup 2
E.2.1 Connecting the Camera and Displaying the Video Signal 2
E.2.2 Saving a the current Image or Recording a Sequence 4
E.2.3 Optimizing the Video Settings in Carrier 2.0 4
E.2.4 Troubleshooting 5
E.3 Uninstall Instructions 6

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User’s Manual Probot D960R3 vii
Warranty
LC Packings (Netherlands) BV, warrants that the products manufactured and sold
by it to be free from defects in material and workmanship for normal use and
service from the date of delivery to original purchaser for a period of one (1) year
from the date of shipment. This limited warranty does not cover, and no
warranty is provided, for parts that by their nature are required to be replaced
periodically as a function of use of the normal operation of the system. These
items include, without limitation: HPLC columns, fuses, tubing, detector sources,
pump piston seals, injector rotors, check valves, filters, any software, etc. In
addition, damage due to corrosion, misuse, negligence, accident, alteration of the
system or repair by an unauthorized individual is not covered by the warranty. It
is understood that the performance characteristics of the instrument require that
the mobile phase is degassed with He or vacuum degassed as described in the
User’s Manual.
This warranty covers products sold under the LC Packings trademark. If a
different warranty than the above is indicated in the sales literature, the warranty
indicated in the sales literature will prevail. If the system includes equipment
supplied by LC Packings but manufactured by a third party, LC Packings makes
no warranty of any kind, express or implied, including, without limitation, any
warranty of merchantability or fitness for a particular purpose. LC Packings will
make available to you, to the extent permitted, the warranties of the
manufacturer of the relevant equipment following your timely written request.
If any product covered by this warranty becomes defective during the warranty
period, it will be repaired or replaced by LC Packings at no charge to the
customer (the repair/replace decision is solely at the option of LC Packings). All
warranty requests must be received by LC Packings during the warranty period.
LC Packings will pay for surface transportation to the applicable LC Packings
Office (North America – Sunnyvale CA, Europe and Asia - Amsterdam, the
Netherlands), if the instrument proves defective within thirty (30) days from the
date of shipment (this does not include air freight, drayage, labor, crating
charges, customs clearance charges, etc.). The user should carefully follow the
directions indicated on the Return Goods Instruction Sheet in the User’s Manual.
After thirty days, all transportation costs will be at the expense of the customer.

Warranty
viii D960R3 User’s Manual Probot
Liability
Under no circumstances shall LC Packings be liable for damage to persons or
property. This warranty is the only warranty given by LC Packings with respect
to products and software provided with the products and is given in lieu of all
other warranties, express or implied, including, without limitation, any warranty
of merchantability or fitness for a particular purpose.
Your exclusive remedies and LC Packings’s sole liability for any non-conformity
or defect in the products and such software will be those expressed herein.
Under no circumstances will LC Packings’s liability arising from the performance
or failure to perform of any product or software, in contract, in tort (including
negligence), or otherwise, exceed the purchase price of the product and
software. In no event will LC Packings be liable, in contract, in tort (including
negligence), or otherwise for special, incidental, consequential or analogous
damages, including, without limitation, damages resulting from loss of use, loss
of profits, loss of business or loss of goodwill, even if LC Packings has been
advised of the possibility of such damages.
This warranty comprises the entire warranty between LC Packings and the
customer. It overrides any warranty related language that may appear in the
customer purchase order or other documentation provided by the customer.
This warranty shall be governed by, and construed and enforced in accordance
with, the laws of the Netherlands. It is non-transferable and shall run to the
benefit of the original purchaser only. Any change, alteration or amendment to
this warranty is not valid unless it has been approved in writing by an officer of
LC Packings.
North America Europe and Asia
LC Packings / Dionex LC Packings (Netherlands) BV
A Dionex Company
500 Mercury Drive Abberdaan 114
Sunnyvale, CA 94088-3603 1046 AA Amsterdam
USA The Netherlands
Technical Call Center Phone: + 31 20 683 9768
USA/CA: [phone] Fax: + 31 20 685 3452

User’s Manual Probot D960R3 ix
Instructions for Returning Instruments
Before you return any item for repair, please contact the nearest LC Packings
office or its local distributor for instructions and obtain a return authorization
number and the ‘Health and Safety Form’ (if applicable).
Pack the equipment carefully, preferably in its original shipping container and
ship it to the LC Packings Service Department, using the appropriate address.
North America Europe and Asia
LC Packings / Dionex LC Packings (Netherlands) BV
A Dionex Company
500 Mercury Drive Abberdaan 114
Sunnyvale, CA 94088-3603 1046 AA Amsterdam
USA The Netherlands
Technical Call Center Phone: + 31 20 683 9768
USA/CA: [phone] Fax: + 31 20 685 3452
IMPORTANT:
1) Make certain that the return authorization number together with the HEALTH
AND SAFETY form (if applicable) is attached outside of the package so that
we can properly track and account for your system.
2) Please include the following
a) Company letterhead with the following information.
Your Name
Complete Mailing Address
Telephone Number, fax number and e-mail address
Return Authorization Number
A detailed description of the problem.
The name of the LC Packings personnel to whom you have
spoken to regarding the problem
Return Shipping Information (if appropriate)
b) Relevant chromatograms
c) A purchase order (if the system is not in warranty)
Note: The completed and signed HEALTH AND SAFETY form must be returned
to LC Packings service department (fax or mail) prior to the return of any
component, or attached outside the shipping package. In addition, the provided
RMA number must be clearly marked on the outside of the shipping package.
Failure to complete and return this form will result in the return of the unopened
package. The parts will not be inspected nor will credit be issued.

Instructions for Returning Instruments
x D960R3 User’s Manual Probot
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User’s Manual Probot D960R3 xi
Warnings
The Danger sign, Warning sign and the Caution sign shown below are included in
various locations in this manual. These signs provide the following information:
DANGER Danger: The information in a danger statement relates to a procedure, practice
condition or action that if not done correctly or adhered to could lead to personal
injury or loss of life.
WARNING
Warning: The information in a warning statement relates to a procedure, practice
condition or action that if not done correctly or adhered to could lead to severe
injury and/or damage or destruction to parts or all of the equipment.
CAUTION Caution: The information in a caution statement relates to a condition that could
lead to damage to equipment and/or lead to invalid analytical results.
Note: The information in a note statement relates to important information that
should be read and understood before continuing.
Safety Precautions
Note: The following precautions should be followed to minimize the possibility of
personal injury and/or damage to property.
Note: Make certain that you are familiar with the contents of this manual before
working on the system.
The Probot Micro Fraction Collector is typically incorporated into an LC/MS
system. The user should follow all safety precautions, warnings, etc provided by
the manufacturer of the system(s), in addition note the items presented below:
1) Install the system in a well-ventilated laboratory. If the mobile phase includes
volatile or flammable solvents, make certain that they are not allowed to
enter the workspace.
2) If the mobile phase includes volatile or flammable solvents, avoid open
flames and sparks.

Warnings and Safety Precautions
xii D960R3 User’s Manual Probot
3) If a leak occurs, turn off power to the instrument and remedy the situation
immediately.
4) All components of the system should be plugged into a common power line
that is directly connected to a true ground.
5) Always replace blown fuses with fuses of the same size and rating indicated
on the fuse holder and panel. Refer to Section 3.3.8 of this manual for more
information on how to replace fuses.
6) Repair or replace faulty power cords and all communication cables.
7) Many organic solvents and buffers are toxic. Make certain that you know the
toxicological properties of all mobile phases that you are using.
8) The toxicological properties of many samples may not be well known. If you
have any doubt about a sample, treat it as if it contained a potentially
harmful substance.
9) Wear protective eye goggles when handling mobile phases or operating the
instrument. An eye wash facility and a sink should be close to the unit. If
any mobile phase is splashed on the eyes or skin, wash the affected area
and seek medical attention.
10) Dispose of all waste mobile phase in an environmentally safe manner that is
consistent with all local regulations. Do not allow flammable and/or toxic
solvents to accumulate. Follow a regulated, approved waste disposal
program. Never dispose flammable and/or toxic solvents through the
municipal sewage system
11) Wear protective eye goggles when handling fused silica tubing (i.e.
installation, cutting etc.)
12) If a buffer is used as a part of the mobile phase, flush the system with
several volumes of a methanol/water (50/50) solution before it is shut down.
This will prevent salt buildup inside the unit.
13) Do not use the instrument in ways other than those indicated in the
instructions given in this manual.

User’s Manual Probot D960R3 xiii
DECLARATION OF CONFORMITY
We LC Packings Nederland BV
A Dionex Company
Abberdaan 114
1046 AA Amsterdam
The Netherlands
declare that our product
ProbotMicro Fraction Collector
is in confirmation with the following documents:
# EEC directives 89/392, incl. 91/368 and 93/44 (machine safety) and EEC
directives 73/23 and 93/68 (low voltage safety), applied with the following
standard:
EN61010-1 Safety requirements for laboratory equipment
(Class I, Installation cat. II, Pollution degree II)
WARNING
LC Packings will not accept any liability for damages direct or indirect
caused by connecting this instrument to devices which do not meet relevant
safety standards.
# EEC directives 89/336 and 92/31 (EMC requirements), applied with the following
standards:
EN 50081-1 Generic emission standard
EN 50082-1 Generic immunity standard
EN 61000-3-2 Harmonic current emissions
Use shielded cables and connectors for all remote connections.
Amsterdam, January 11, 2001
Robert van Ling, QA manager

CE Declaration
xiv D960R3 User’s Manual Probot
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User’s Manual Probot D960R3 1-1
CHAPTER 1 Introduction
1.1 Features of the ProbotMicro Fraction Collector
The LC Packings Probot Micro Fraction Collector is a robotic fraction collector
that can be used to collect samples from a capillary HPLC or CZE system and
deposit onto targets such as a MALDI mass spectrometer target, a PVDF
membrane (e.g. for protein sequencing), a nitrocellulose membrane or a micro
titer plate.
The heart of the unit is a robotic table that can be positioned in the X, Y and Z
directions by means of 3 stepper motors under the control of a personal
computer and the Carriersoftware package. The table can be driven in either
a linear or circular motion and the distance from point to point, the time spent at
a point and the speed of movement are readily programmed. A reference position
is defined by the system, and the user can select a starting position as well as a
park position (where the plate should be moved to during rest periods). Various
adapter tables are available for carrying the different targets.
When the Probot Micro Fraction Collector is employed with the µCarrier
software, the system provides the following features:
High precision X-Y-Z movement of the table – any position within the working
area can be programmed with a resolution down to ± 2 µm.
Smooth operation – results in no vibration of the needle, which is essential for
nanoliter spotting and smooth UV-detection.
Short capillary connection between outlet and needle – extremely low dwell
volume.
Fully automated adding of matrixes or make-up solutions – simplifies spotting
Capillary/Nano LC fractions onto MALDI targets. The matrix can either be
added before the needle (µ-Tee) or co-axially at the needle tip (standard Tee).
Dual-Collection mode - a fraction can be split between two sample carriers
simultaneously, i.e., a MALDI/MS target and a well plate, or a PVDF
membrane. The split ratio is programmable and all sample vessels, including
1536 well plates can be used.

Introduction
1-2 D960R3 User’s Manual Probot
1.2 General Design of the Probot Micro Fraction Collector
The general configuration of the system (with sample deposition on a MALDI
target and the Dosage Module option) is presented in FIGURE 1-1.
X
Flow Cell
Waste
Fill
Wash
Y
Z
Needle
Capillary
µ
-Tee
Table
Dosage
Module
Rotary
Valve
Syringe
From LC/CZE
System
Probot

UltiMateUV Detector
Vials
Union
Target
FIGURE 1-1 General Configuration of the Probot Micro Fraction Collector
The Probot Micro Fraction Collector is supplied with a µ-Tee connection
(FIGURE 1-1). The lower end of the needle Tee holds a hollow needle in which a
fused silica capillary is mounted, the horizontal inlet is used to add reagent (e.g.
a matrix solution) to the sample using the Dosage Module and the upper
connection is connected to the LC/CZE system (e.g. the capillary flow cell outlet
of the UltiMate UV detector).

Introduction
User’s Manual Probot D960R3 1-3
The following options are available for the Probot:
Manual Control Unit – allows for moving the sample table manually.
Dosage Module – The dosage module consists of a 4 way rotary valve with a
syringe drive and a 100 L syringe. It can be used in different modes:
-Continuous Flow Mode – a matrix solution/reagent is added on a
continuous basis (nL/min to µL/min).
-Dosage Mode –a user-defined volume is added to each sample.
-Wash Mode – the needle can be entirely flushed to avoid crystallization of
the matrix solution.
µ-Tee for Matrix Addition – allows for adding the matrix solution before the
needle directly in the Nano flow path (default configuration).
Standard Needle Tee – allows for adding the matrix solution through the
hollow needle co-axially at the needle tip (supplied with the instrument).
Camera Option – A camera kit (P/N 160590) is available which allows for easy
monitoring of the needle and target(s) in conjunction with µCarrier 2.x or a
standalone monitor.
Pipetting Option – The pipetting option (P/N 161072) allows for pipetting from
a source plate (e.g. a well plate) to a destination plate (e.g. MALDI target). It
consists of a Teflon tube (45 cm, I.D. 250 m) with fittings and a fused silica
capillary (15 cm, O.D. 280 m, I.D. 100 m).
Waste Removal Option –allows the user to remove undesired portions of the
separation (‘waste’) without the need to spot them onto the target. A vacuum
can be applied to draw the solvent into a waste container.

Introduction
1-4 D960R3 User’s Manual Probot
1.3 Front View of Probot
Light Pencil
Light Switch
Rotary Valve LEDs
µ-Tee
with
Needle
Power Switch
Dosage
Module
(optional)
Target
X-Y-Z Drive
Table
Adapter
Camera
(optional)
FIGURE 1-2 Front View Probot - Doors and Needle Cover Removed
1.4 The Manual Control Unit
Mode LEDs
X Axis Control Buttons
Y Axis Control Buttons
Z Axis Control Buttons
Mode Buttons
FIGURE 1-3 Manual Control Unit

Introduction
User’s Manual Probot D960R3 1-5
1.5 Rear View of Probot
POWER
Connector
VIDEO out
RELAY
Connector
INTERLOCK
Connector
RS-232
Connector
START
Connector
ANALOG IN
Connector
TTL IN
Connector
REMOTE
Connector
FIGURE 1-4 Rear View Probot
1.6 Control of the Probot Micro Fraction Collector
The Carrier Application Designer software, which is a Windows® based program
is used to control all functions of the Probot Micro Fraction Collector and to
support time based fraction collection. It can be used for peak collection in
conjunction with any software package that allows for peak detection (e.g. the
LC Packings CHROMELEONSoftware) and that can provide control signals (e.g.
contact closure signals). Fractionating can then be controlled by the TTL inputs
“Peak Start’, ‘Peak Top’ and ‘Peak End’ of the Probot Micro Fraction Collector.

Introduction
1-6 D960R3 User’s Manual Probot
1.7 About this Manual
This manual describes the LC Packings Probot Micro Fraction Collector and
includes the following information:
CHAPTER 2: Installation and Getting Started describes how to install the
Probot and the Carrier Software. It includes the various steps that should be
performed to setup the system, defines how to define a table and targets, set up
a task list with the Carrier software and prepare the system for operation.
CHAPTER 3: Maintenance and Troubleshooting describes a variety of
maintenance procedures to optimize the performance of the Probot Micro
Fraction Collector. In addition, it discusses how the operator can determine the
cause of a difficulty in the operation of the instrument and includes a list of
spare/replacement parts.
CHAPTER 4: Specifications presents the specifications of the Probot.
In addition, a number of appendices discussing different topics in more details
are included:
APPENDIX A: Dosage Module Purge Routine provides a step-by-step procedure
of how to purge the syringe and tubing of the dosage module properly.
APPENDIX B: Peak Collection in Conjunction with CHROMELEON® Software
discusses the setup of the CHROMELEON and the µCarrier software packages
and the Probot for peak collection.
APPENDIX C: Table Alignment Instructions discusses alignment procedures that
are recommended whenever the instrument has been relocated or shipped.
APPENDIX D: The Vacuum Waste Removal Option describes the features and
usage of this option in conjunction with the CHROMELEON software.
APPENDIX E: Installing and Using the BelkinUSB Video Adapter describes the
installation and usage of the Belkin VideoBusII Image Capture Device.
If you are using the Probot Micro Fraction Collector in conjunction with the
LC Packings UltiMate System and/or CHROMELEON software, please refer to the
documentation provided with these products for supplemental information.
The Probot Micro Fraction Collector is used with equipment supplied by other
manufacturers (e.g. a mass spectrometer). The manuals provided with these
systems should be consulted for additional information (e.g. interfacing).
The manual provides basic information about how to install, setup and use the
µCarrier software package. In addition, it provides information about defining
tables and targets and how to control the Probot Micro Fraction Collector. For
more detailed information how to use the Carrier software package refer to the
software manual provided on the Carrier installation CD ROM.
Note: This manual covers the Probot with S/N 1120 or higher and with the
standard Needle-Tee or the Micro-Tee installed (factory installed since January
2004). If you are using instruments with a serial number lower than 1120, please
refer to the documentation provided with the instrument.