Knauer Azura P 6.1L User manual

HPLC
Pump P 6.1L
User Manual
V6890

3TableofContents
KNAUER P 6.1L User Manual V6890 Version 1.2
Table of Contents
Note For your own safety, read the manual and always observe the
warnings and safety information on the device and in the 
manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Definition of Personal and Material Damage . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Capillary and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Pre-Installed Capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Integrating the Pump into an HPLC Flow System . . . . . . . . . . . . . . . . . . . . 18
Isocratic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . 19
Binary Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting the Solvent Selection Valve to the Solvent . . . . . . . . . . . . . . . . 21
Quaternary Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the 4-Channel Degasser to the Solvent . . . . . . . . . . . . . . . . . . 23
Connecting the Valve Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Connecting the Piston Backflushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4TableofContents
KNAUER P 6.1L User Manual V6890 Version 1.2
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Connecting the Device to a Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . 29
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . 29
Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Remote Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Explanations for Remote Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Events Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Explanations for Events Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Wiring the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
De-Aerating the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Control with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Control with Control Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Control with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Functionality Tests IQ and OQ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Possible Problems and Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Contact with the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Putting the Pump Out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Disconnecting from Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Preparing the Pump Head for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Dismounting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5TableofContents
KNAUER P 6.1L User Manual V6890 Version 1.2
Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Removing the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Cleaning the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Installing the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Replacing the Inline Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Removing the Inline Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Inserting the new Inline Filter Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Replacing the Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
P 6.1L Isocratic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
P 6.1L Binary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
P 6.1L Quaternary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Flow Rate and Maximum Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
P 6.1L General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Legal Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
To whom it may concern In case you prefer a French language user manual for this prod-
uct, submit your request including the corresponding serial
number via email or fax to KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit
contacter KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

KNAUER P 6.1L User Manual V6890 Version 1.2
6IntendedUse
Intended Use
Note Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equip-
ment of the device could fail.
Description The pump P 6.1L is suitable for analytical and semi-preparative
applications.
Scope of application The device can be used in the following areas:
Biochemistry analysis
Chemical analysis
Food analysis
Pharmaceutical analysis
Environmental analysis
Device Variants
The P 6.1L is available in three different options:
Isocratic pump
Binary pump (HPG pump)
Quaternary pump (LPG pump)
All variants are available as a standard version made of stainless
steel or as a bio-inert version made of ceramics with PEEK capil-
lary, PEEK connectors, and PEEK venting screw.
Front View
Isocratic pump The isocratic pump is equipped with a pressure sensor with an
integrated inline filter and a venting screw.
Legend
1Pressure sensor
2Pump head
Fig. 1 Isocratic pump - front view
12

KNAUER P 6.1L User Manual V6890 Version 1.2
7IntendedUse
Binary pump The binary pump consists of two pump drives and a 2-channel
degasser with a Solvent Selection valve. A pressure sensor with an
integrated inline filter and a venting screw are also part of the
pump.
Quaternary pump The quaternary pump consists of a pump, a valve block and a 4-
channel degasser. A pressure sensor with an integrated inline fil-
ter, a venting screw, and a mixer are also part of the pump.
Rear View
On the back of pump, the connectors for LAN and power chord
are located as well as the serial number of the device an the
power switch (on/off).
Service interface Please note that the service interface is solely used for repair and
maintenance tasks performed by service technicians.
Legend
1Pressure sensor
2Mixer
3Pump head
4Degasser
5Solvent Selection
valve
Fig. 2 Binary pump - front view
12345
Legend
1Pressure sensor
2Mixer
3Pump head
4Valve block
5Degasser
Fig. 3 Quaternary pump - front view
12 3 4 5

KNAUER P 6.1L User Manual V6890 Version 1.2
8IntendedUse
Side View
On the side of the pump, the Control Unit (see "Control with
Control Unit” on page 37) connector is located.
Features
The pump is used to convey liquids, either in an HPLC system or
as a stand-alone device. Liquids are conveyed either with con-
stant flow or with constant pressure. The flow and pressure,
which liquids can be conveyed with, depend on the pump head.
Pump Heads Pump heads for several areas of application are available for the
pump P 6.1L.
Legend
1Serial number
2Interface for the
Technical Support
3Pin header
4LAN port
5Power connection
and power switch
Fig. 4 Rear view (example)
123
45
Legend
1Control Unit port
Fig. 5 Side View
1
Standard Bio-inert
Size 5 ml, 10 ml and 50 ml 10 ml and 50 ml
Material Pump head with stainless
steel inlays
Pump head with ceramic
inlays

KNAUER P 6.1L User Manual V6890 Version 1.2
9IntendedUse
Every pump head is equipped with an RFID chip. It is used to
monitor and save all important parameters and settings. RFID
technology offers the following advantages:
Software automatically recognizes the parameters of the
pump head
All service-relevant data of the pump head is stored
Mixer Volume of the mixer is 100 μl. The mixer is exchangeable and
can be replaced by mixers with a volume of 50 μl or 200 μl .
The parameters necessary for the mixer are set via Control Unit
or via chromatography software.
Piston backflushing The piston backflushing function automatically flushes the rear
piston area of the pump head upon switch-on and in continuous
mode.
Upon switch-on: The rear piston area of the pump head is
automatically flushed for 15 seconds.
In continuous mode: The rear piston area of the pump head
is flushed automatically every 15 minutes, for 15 seconds.
PMax Mode This mode protects the column from excessive fluctuations in
pressure. If the maximum pressure is reached, the user can
decide if the pump should be switched off or continue running
with constant pressure. You find a detailed description on how
to use this option in the respective software manual.
GLP data The Control Unit, the Mobile Control and the different software
products (e.g. ClarityChrom®or OpenLAB®) can be used to dis-
play or read out the GLP data of the pump. You find a detailed
description on how to display or read out GLP data in the
respective user manuals.
Optional accessories The following optional accessories are available:
Pump head cooler for the pump head
Control Unit
Mobile Control
Solvents
Even small quantities of other substances, such as additives,
modifiers, or salts can influence the durability of the materials. If
there is any doubt, contact the Technical Support of the manu-
facturer.
Scope of Application HPLC
Dosing applications with
non-aggressive media
Purification/FPLC
Ion chromatography
Standard Bio-inert
Stainless steel Bio-inert
Size 50 μl, 100 μl, 200 μl 250 μl
Pressure 1000 bar 400 bar

KNAUER P 6.1L User Manual V6890 Version 1.2
10 Intended Use
Suitable solvents
Acetate buffer solutions
Acetone at 4 °C–25 °C (39.2 °F–77.0 °F)1
Acetonitrile2
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Chloroform
Dilute acetic acid (e.g. 0.1–1 %) at 25 °C/77.0 °F
Dilute ammonia solution
Dilute sodium hydroxide (1 M)
Ethyl acetate
Ethanol
Formiate buffer solution
Isopropanol
Methanol
Phosphate buffer solutions (0.5 M)
Toluol
Water
1. valid for the specified temperature range
2. not recommended in combination with PEEK small parts and PEEK capillary
Less suitable solvents
Diethylamine (0.1 %) (DEA)
Dilute phosphoric acid
Dimethyl sulfoxide (DMSO)
Methylene chloride1
Slightly volatile solvents
Tetrahydrofuran (THF) 1
Triethylamine (0.1 %) (TEA)
Trifluoroacetic acid (0.1 %) (TFA)
1. not recommended in combination with PEEK small parts and PEEK capillary
Not suitable solvents
Concentrated mineral and organic acids
Concentrated bases
Halogenated hydrocarbons, e.g. Freon®
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles

KNAUER P 6.1L User Manual V6890 Version 1.2
11 Scope of Delivery
Flushing solution These are the recommended flushing solutions, e.g. for the pis-
ton backflushing:
Water
Mixture of 80 % water and 20 % ethanol
Isopropanol
Scope of Delivery
Note Only use spare parts and accessories made by KNAUER or a
company authorized by KNAUER.
Safety for Users
Professional Group The user manual addresses persons who are qualified as chemi-
cal laboratory technicians or have completed comparable voca-
tional training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a
limited extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by
the company KNAUER or an authorized company.
Solvents not suitable for degassers
Azides
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Concentrated mineral and organic acids
Concentrated bases
Dilute sodium hydroxide (1 M)
Halogenated hydrocarbons, e.g. Freon®
Hexafluoroisopropanol
Hexanes (60 % n-Hexane)
Hydro fluoro solvents
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles
Delivery Pump P 6.1L
Power cable
AZURA accessories kit, P 6.1L accessories kit
User manual English/German
Installation Qualification

KNAUER P 6.1L User Manual V6890 Version 1.2
12 Safety for Users
If you do not belong to this or a comparable professional group,
you may not perform the work described in this user manual
under any circumstances. In this case, please contact your supe-
rior.
Safety Equipment When working with the device, take measures according to lab
regulations and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifica-
tions in the user manual
National and international regulations pertaining to labora-
tory work
Original spare parts, tools, and solvents made or recom-
mended by KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident
insurance companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other
areas of the HPLC system.
Only use a given PEEK fitting for one specific port and never
re-use it for other ports. Always install new PEEK fittings on
each separate port.
Follow KNAUER or manufacturer's instructions on caring for
the columns
More safety-relevant information is listed in alphabetical order in
the following table:
Topic Explanations
flammability Organic solvents are highly flammable.
Since capillaries can detach from their
screw fittings and allow solvent to escape,
it is prohibited to have any open flames
near the analytical system.
solvent tray Risk of electrical shock or short circuit if
liquids get into the device's interior. For
this reason, place all bottles in a solvent
tray.
solvent lines Install capillaries and tubing in such a way
that liquids cannot get into the interior in
case of a leak.
leaks Regularly check if any system compo-
nents are leaking.

KNAUER P 6.1L User Manual V6890 Version 1.2
13 Safety for Users
Where is use of the
device prohibited?
Never use the system in potentially explosive atmospheres with-
out appropriate protective equipment. For further information,
contact the Technical Support of KNAUER.
Decommissioning the
Device Securely
At any time, take the device completely out of operation by
either switching off the power switch or by pulling the power
plug.
Opening the Device The device may be opened by the KNAUER Technical Support or
any company authorized by KNAUER only.
Definition of Personal and Material
Damage
Possible dangers related to the device are divided into personal
and material damage in this user manual.
Decontamination
Contamination of devices with toxic, infectious or radioactive
substances poses a hazard for all persons during operation,
repair, sale, and disposal of a device.
power cable Defective power cables are not to be used
to connect the device and the power sup-
ply system.
self-ignition point Only use eluents that have a self-ignition
point higher than 150 °C under normal
ambient conditions.
power strip If several devices are connected to one
power strip, always consider the maxi-
mum power consumption of each device.
power supply Only connect devices to voltage sources,
whose voltage equals the device's volt-
age.
toxicity Organic eluents are toxic above a certain
concentration. Ensure that work areas are
always well-ventilated! Wear protective
gloves and safety glasses when working
on the device!
Topic Explanations
Category Explanations
DANGER! Lethal or very serious injuries can occur.
WARNING! Serious or moderate injuries can occur.
CAUTION! Moderate injuries can occur. Device defects
can occur.

KNAUER P 6.1L User Manual V6890 Version 1.2
14 Symbols and Signs
DANGER! 
Health danger if getting in contact with toxic,
infectious or radio-active substances. 
Before disposing of the device or sending it away
for repair, you are required to decontaminate the
device adequately.
All contaminated devices must be properly decontaminated by a
specialist company or the operating company before they can
be recommissioned, repaired, sold, or disposed of. All materials
or fluids used for decontamination must be collected separately
and disposed of properly.
Symbols and Signs
The following symbols and signs can be found on the device, in
the chromatography software or in the user manual:
Symbol Meaning
Warning signs High-voltage hazard
Electric shock hazard
Danger caused by potentially toxic substances
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
General warning sign, moderate injuries can
occur and also damages to system, device, or
components.
Leak hazard, damage to device can occur.
Note maximum weight-loading of the leak tray
during transport, installation and operation.
CE mark A device or system marked with CE fulfills the
product specific requirements of European
directives. This is confirmed in a Declaration of
Conformity.

KNAUER P 6.1L User Manual V6890 Version 1.2
15 Installation
Installation
The chapter Installation describes all preparatory steps prior to
the start-up. If you encounter difficulties during installation, con-
tact the Technical Support.
Transport
Carefully prepare the device for transport or storage. If you want
to return your device to KNAUER for repairs, enclose the Service
Request Form which can be downloaded from our website.
Device data For a secure transport, note the weight and dimensions of the
pump (see Technical Data).
CAUTION! 
Damage to the device by lifting on protruding
housing parts. 
Lift the device on the side of the housing only.
Lifiting Clasp the device at its side panels and lift it out of the packag-
ing. Do not hold onto front cover or leak tray.
Testing seals in Canada and the USA at nation-
ally recognized testing centers (NRTL). The cer-
tified device or system has successfully passed
the quality and security tests.
Symbol Meaning
Contact data Phone [phone]
Fax [phone]
E-mail [e-mail]

KNAUER P 6.1L User Manual V6890 Version 1.2
16 Installation
Operating Environment
Ambient conditions Only if the requirements for ambient conditions are met, can the
intended use be ensured.
Earth quake regions If the device is set up in a region where earth quakes are com-
mon, secure the device at the two fixing points 1. The fixing
points are situated on both sides of the device.
Setup
Prior to setting up the device, find a suitable place according to
the requirements and remove the packaging. The requirements
and a description can be found in the following section.
Sunlight: Protect the device against direct exposure to sun-
light.
AC system: Set up the device at a location not exposed to
air drafts.
Vibration: Do not set up the device in the vicinity of other
machines that cause floor vibrations.
Installation site:
Position the device on a level and even surface.
Height above sea level: maximum 2000 m
Weight 11.5 kg isocratic
14.1 kg binary
12.7 kg quaternary
Dimensions
(width × height ×
depth)
361 mm x 208.2 mm x 523 mm
Power supply Input 100–240 V
Output 50–60 Hz
Maximum power
consumption
100 Watt
Humidity < 90 %, non-condensing
Temperature 10–40 °C (50–104 °F)
Fig. 6 Fixing points on the device
1

KNAUER P 6.1L User Manual V6890 Version 1.2
17 Installation
Unpacking
Prerequisite Check packaging for damage caused during transportation.
Tool Utility knife
CAUTION! 
Damage to the device by lifting on protruding
housing parts. 
Lift the device on the side of the housing only.
Next steps Store packaging and keep the included packing list for repeat
orders.
Capillary and Fittings
All tubing and capillary, which connect the components of the
pump, are pre-installed. Only the solvent bottles have to be con-
nected and the pump has to be integrated into the flow of the
HPLC system.
Connecting the Solvent Bottles
To connect the solvent bottles, tubing with pre-installed solvent
filters is used. The tubing is connected to the device with flange-
less fittings.
Note Do not use tools so as to not damage the fittings.
Procedure Process
1. Set-up the package in such a way that you can read the
label. Using the utility knife, cut the adhesive tape and
open the packaging.
2. Remove the foam insert. Take out the accessories kit and
the manual.
3. Open the accessories kit and check the scope of delivery. In
case any parts are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging
and place it on its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during
transport. In case you notice any damage, contact the
Technical Support.
6. Place the device in its site of operation and remove protec-
tive foil.

KNAUER P 6.1L User Manual V6890 Version 1.2
18 Installation
Pre-Installed Capillary
Pre-installed stainless steel and PEEK capillary is color-coded
according to its inner diameter.
Note PEEK capillary is not suitable for use with Acetonitrile. Acetoni-
trile can cause capillary to crack or rupture.
Integrating the Pump into an HPLC Flow Sys-
tem
The pump can be integrated into an HPLC flow system by con-
necting the pressure sensor (isocratic version) or the mixer
(binary or quaternary version) and the HPLC system with capil-
lary.
Procedure Process Figure
1. Slide the flangeless fit-
ting 1over the tub-
ing.
2. Slide the lock ring 2
over the tubing, cap
with the sealing ring
3. Note the direction
of the lock ring, other-
wise the sealing ring
could be damaged.
The thicker end of the
lock ring must point
into the direction of
the fitting. Fasten the
polymer seal.
Fig. 7 Set-up of a flangeless fit-
ting
3. Manually fasten the
flangeless fitting 4to
the device.
Fig. 8 Fully assembled flange-
less fitting
123
4
Color Material Inner diameter
red marker Stainless steel 0.1 mm
blue marker Stainless steel 0.25 mm
black marker Stainless steel 0.45 mm
blue stripes PEEK 0.25 mm
orange stripes PEEK 0.5 mm

KNAUER P 6.1L User Manual V6890 Version 1.2
19 Installation
Note To integrate the pump into a system, note the ambient condi-
tions found in the sections Operating Environment and Techni-
cal Data as well as the ambient conditions of other devices to be
integrated into that system.
CAUTION! 
Damage to the pump head caused by overtight-
ened capillary fittings! 
Note the torque of the fittings:
- 5 Nm for stainless-steel fittings
- 0.5 Nm for PEEK fittings
Tool Torque wrench
Procedure Stainless steel fittings are tightened with 5 Nm, PEEK fittings are
tightened with 0.5 Nm.
Isocratic Pump
The figure shows the installation plan for capillary and tubing on
the isocratic version of the pump. If you own a different version
of the pump, see the corresponding chapter for installation
instructions.
The gray lines represent the connection of the solvents to the
pump and the black lines represent the connection of the piston
backflushing.
Piston backflushing The flushing solution is re-used. Since the flow path is circular,
only one bottle is used for the flushing solution.
Solvent flow path The pump head takes the liquid in from the bottle and conveys
it to the pressure sensor.
Connecting the Pump Head to the Solvent
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
Material Flangeless fitting
Capillary layout
Fig. 9 Installation plan for the isocratic pump

KNAUER P 6.1L User Manual V6890 Version 1.2
20 Installation
CAUTION! 
Damage to the pump head possible!
Remove the cap fittings from the inlet and outlet
of the pump head prior to use!
Next steps Integrate the pump into the HPLC flow system.
Binary Pump
The figure shows the installation plan for capillary and tubing on
the binary version of the pump. If you own a different version of
the pump, see the corresponding chapter for installation instruc-
tions.
The gray lines represent the connection of the solvents to the
pump and the black lines represent the connection of the piston
backflushing.
Piston backflushing The flushing solution is re-used. Since the flow path is circular,
only one bottle is used for the flushing solution.
Procedure Process Figure
1. Slide the flangeless fit-
ting onto the tubing.
2. Insert the tubing into
the free inlet 1on the
bottom of the pump
head.
3. Tighten the fitting by
hand.
Fig. 10 Solvent line on the pump
head
1
Capillary layout
Fig. 11 Installation plan for the isocratic pump

KNAUER P 6.1L User Manual V6890 Version 1.2
21 Installation
Solvent flow path Every pump head can operate with two different solvents. Both
solvents are connected with the Solvent Selection valve. The sol-
vents are conveyed into one pump head each and combined in
the pressure sensor. The pressure sensor is connected to the
mixer. The mixer is connected to the HPLC system.
Connecting the Pump Head to the Solvent
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
Material Flangeless fitting
CAUTION! 
Damage to the pump head possible!
Remove the cap fittings from the inlet and outlet
of the pump head prior to use!
Next steps Integrate the pump into the HPLC flow system.
Connecting the Solvent Selection Valve to the
Solvent
Note The Solvent Selection valve is readily connected to the 2-chan-
nel degasser.
The Solvent Selection valves allows selecting from two different
solvents for each solvent channel, without having to re-install
the tubing. For both solvent channels A and B, one of two sol-
vents can be selected. Solvent A is connected to inlets A1 and
A2, solvent B is connected to inlets B1 and B2.
Prerequisite The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
Material Flangeless fitting
Procedure Process Figure
1. Slide the flangeless fit-
ting onto the tubing.
2. Insert the tubing into
the free inlet 1on the
bottom of the pump
head.
3. Tighten the fitting by
hand.
Fig. 12 Solvent line on the pump
head
1