Covaris E220-plus Manual

E220 & LE220 (-plus, R-plus, Rsc)

Setup & Instrucon Manual

Instrument and Soware Developed for High-performance

Sample Preparaon using Adapve Focused Acousc® (AFA®) Energy

For Research Only

Model Numbers: E220-plus, E220R-plus, LE220-plus, LE220R-plus, and LE220Rsc | Soware: SonoLab®10.2

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

UNIVERSAL PRECAUTIONS

Universal Precauons should be followed on all specimen samples, regardless of whether a sample is known to contain

an infecous agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following

government and clinical standards, or their equivalent in the country of use:

• Centers for Disease Control (CDC), Universal Precauons for Prevenon of Transmission of HIV and Other Bloodborne

Infecons, published 1987, updated 1996

• Clinical and Laboratory Standards Instute (CLSI), GP17-A3 Clinical Laboratory Safety; Approved Guideline - Third

Edion, published 2012, ISBN 1-56238-797-9 (Print), ISBN 1-56238-798-7 (Electronic)

• Clinical and Laboratory Standards Instute (CLSI), M29-A4 Protecon of Laboratory Workers from Occupaonally

Acquired Infecons; Approved Guideline, Fourth Edion, published 2014, ISBN 1-56238-961-0 (Print), OSBN

1-56238-962-9 (Electronic)

• Occupaonal Safety and Health Administraon (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens

• Internaonal Standards Organizaon (ISO) 15190:2003, Medical Laboratories – Requirements for Safety

Trademarks

Patented or patent pending and registered or registraon-pending trademarks of Covaris are protected.

Registered names and trademarks used in this document, even when not specically marked as such, are not to be considered

unprotected by law.

WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries.

Teon is a registered trademark of The DuPont Company.

Informaon subject to change without noce

For Research Use Only (RUO)

Not for use in Diagnosc Procedures

Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applicaons

Covaris, Inc.

14 Gill St, Unit H

Woburn, Massachuses

01801-1721 USA

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

Warnings

For the Safety of Operang Personnel:

• Make sure that the equipment is properly grounded. DO NOT operate if it’s not properly grounded.

• The unit is equipped with a power plug appropriate for the desnaon country. DO NOT, under any circumstances, remove the

grounding prong from the power cord.

• DO NOT run an acousc treatment with the enclosure door OPEN or without a water bath - the acousc system will not work.

• If there is any indicaon that the Safety System is not funconing properly, DO NOT operate the equipment. Contact Covaris

Technial Support immediately if this occurs.

• If the equipment is used in a manner not specied by the manufacturer, the protecon provided by the equipment may be

impaired.

To Prevent Damage to the Equipment:

• The instruments are designed to operate in ambient laboratory condions e.g., 19 to 25 °C (66 to 77 °F). DO NOT operate the

instrument in a cold room environment. The system is designed to operate with a water bath and recirculang heater/chiller

apparatus to control sample temperature.

• NEVER run a method without water in the bath since this could cause damage to the transducer. The instrument is equipped

with a water level sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to

operate or the acousc treatment to start unless an adequate volume of water is detected.

• Dislled or deionized water should be used to ll the water bath.

• Unless a Water Condioning System 2.0 (WCS) is acvely employed, empty the water bath and wipe it dry EVERY DAY with a

lint-free cloth. DO NOT leave water in the bath for an extended me since there is no water ltraon or water cleaning system

within the apparatus (unless WCS is employed).

• DO NOT employ isopropyl alcohol, ammonia-based or abrasive cleaners in the system, since these chemical soluons will

damage the acrylic surfaces.

• Establish a standard of operaon and test the equipment periodically, as described in the maintenance secon of this manual.

CAUTION: All AutoFill components have pressure rangs above Covaris’ specied maximum operang pressure which have

been extensively tested. However, if plant DI water is connected to the instrument, a catastrophic failure may result in

substanal plant water spillage. It is the end user’s responsibility to specify and implement appropriate containment/alarm

schema for their facility.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

Warranty

When used in accordance with wrien instrucon and under normal operang condions, the Covaris instruments are guaranteed

to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original delivery by an authorized

representave. Any component proven defecve during the stated period will be repaired free of charge or replaced at the sole

discreon of Covaris, F.O.B., Woburn, Massachuses, U.S.A. provided that the defecve component is returned properly packaged with

all transportaon charges prepaid. The customer is expected to perform basic diagnoscs and component replacement via telephone

support from Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the

customer. A limited warranty as specied may apply to certain components of the equipment.

Warranty Excepons

This warranty is void if failure of the soware or hardware has resulted from accidents, abuse, improper maintenance or repair, or by

misapplicaon from the customer. It is also void if damage is caused by any unauthorized aachments or if modicaons are made to

the equipment. Removing or tampering with the Safety Enclosure will void the warranty, and the customer will assume all liabilies.

This warranty is limited to the original purchaser and is not transferable.

The soware will perform according to the accompanying wrien materials and the medium on which the soware is delivered is

free of defects in materials under normal use and service. The warranty is void if damage has resulted from third party soware not

intended for use with the system.

The high-power focused transducer is designed to give maximal mechanical energy output in water. Permanent damage to the

transducer and electronic circuits could result if the transducer is operated without water. Operaon of the system without water in

the water bath voids the warranty.

Contact Covaris, Inc. if you have quesons or concerns pertaining to the use of our quipment.

Warranty Services

The purchased equipment is covered by a twelve (12) month warranty which includes all the necessary services and support necessary

so end users can successfully operate their equipment. Extended warranes are available at the end of the original 12 month warranty

period.

Services included with the original purchase of the system are:

Technical Support – Ongoing assistance with the operaon or applicaon of the equipment and/or troubleshoong is provided via:

• Telephone

- United States: Tel: +1 781.932.3959 during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,

Eastern Standard Time/EST (UTC−05:00)

- Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,

Greenwich Mean Time/GMT

• E-mail queries to [email protected] or applicaonsupport@covaris.com

Parts Replacement – Replacement of parts (excluding consumables) from normal operaon of equipment are provided on a priority

basis. All labor and shipping charges are included. Failure due to accident, abuse, or improper operaon is not covered.

This manual includes maintenance guidelines and troubleshoong ps.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

Table of Contents

1. Introducon................................................................................................................................................................................................................ 6

1.1 Overview of the User Manual ......................................................................................................................................................................... 6

1.2 E220-plus Focused-ultrasonicator ................................................................................................................................................................. 6

1.3 E220R-plus Focused-ultrasonicator............................................................................................................................................................... 6

1.4 LE220Rsc Focused-ultrasonicator.................................................................................................................................................................. 6

2. Installaon and Setup ............................................................................................................................................................................................... 7

2.1 Assembly............................................................................................................................................................................................................. 7

3. Geng Started........................................................................................................................................................................................................... 11

3.1 Fill the Water Bath ............................................................................................................................................................................................ 11

3.2 Start the System ................................................................................................................................................................................................ 11

3.3 Preparing your System for Use........................................................................................................................................................................ 13

3.4 System Shutdown.............................................................................................................................................................................................. 16

4. AFA Operaon............................................................................................................................................................................................................ 17

5. SonoLab Soware...................................................................................................................................................................................................... 18

5.1 The Method Screen........................................................................................................................................................................................... 18

5.2 The Schedule Screen......................................................................................................................................................................................... 22

5.3 The History Screen............................................................................................................................................................................................ 24

5.4 The About Screen.............................................................................................................................................................................................. 25

5.5 The Admin Screen ............................................................................................................................................................................................. 26

6. Create and Edit Methods ......................................................................................................................................................................................... 30

6.1 Create a New Method ...................................................................................................................................................................................... 30

6.2 Using the Repeat Funcon .............................................................................................................................................................................. 36

6.3 Scanning Treatments (LE220Rsc Only) .......................................................................................................................................................... 39

6.4 Using Drag and Drop with Treatment Steps ................................................................................................................................................. 42

6.5 Modify Exisng Methods ................................................................................................................................................................................. 43

7. System Specicaons................................................................................................................................................................................................ 44

8. Maintenance............................................................................................................................................................................................................... 45

8.1 Cooling Air Intake .............................................................................................................................................................................................. 45

8.2 Water Bath.......................................................................................................................................................................................................... 45

8.3 Transducer........................................................................................................................................................................................................... 45

8.4 Safety System..................................................................................................................................................................................................... 46

8.5 Degassing System.............................................................................................................................................................................................. 46

8.6 Cleaning the System ......................................................................................................................................................................................... 46

8.7 Water Bath.......................................................................................................................................................................................................... 46

9. Troubleshoong ......................................................................................................................................................................................................... 48

10. Appendix................................................................................................................................................................................................................... 50

Appendix A: Using SonoLab Consumable-based Method Selecon................................................................................................................ 50

Appendix B: Using SonoLab Posion Restricons .............................................................................................................................................. 51

Appendix C: SonoLab 8.x Data Converter ........................................................................................................................................................... 53

Appendix D: E220 (-plus and R-plus) Series Site or Transport Preparaon ................................................................................................... 54

Appendix E: Installing SonoLab 10 Soware....................................................................................................................................................... 55

Appendix F: Humidity and Water Management for the Covaris E220-plus................................................................................................... 58

Appendix G: Connecng AutoFill to Plant Water ............................................................................................................................................... 59

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

1.1 Overview of the User Manual

This manual contains the operaon and maintenance instrucons for the Covaris E220-plus, E220R-plus, LE220-plus, LE220R-plus,

and LE220Rsc Focused-ultrasonicators. This document contains informaon that is essenal to the proper use and care of your

equipment. Should any unforeseen problems occur with the normal operaon of the equipment, immediately contact your Covaris

Technical Support representave.

The following denions apply in this manual:

1.2 The E220- and LE220-plus Focused-ultrasonicator

The E220- and LE220-plus Focused-ultrasonicators are based on the Covaris Adapve Focused Acouscs (AFA) technology. These

instruments are designed to provide researchers with an integrated stand-alone tool to be used in a laboratory environment and are

ideal for those who work with ssue homogenizaon, cell lysis, DNA or chroman shearing, and other sample preparaon steps. The

E220-plus treats samples sequenally, one sample at a me. The LE220-plus treats a column of samples simultaneously.

This system is comprised of the:

• Treatment System which delivers the acousc energy to the sample

• Safety System which protects users from inadvertently contacng the high intensity acousc energy

• Computer and Applicaon Soware which provides an interface to control the device via the API

• Water Management System which consists of a cooler/heater and a UV treatment chamber with parculate lter to maintain

water quality in the instrument bath

1.3 The E220R- and LE220R-plus Focused-ultrasonicator

In addion to the features of the E220- and LE220-plus described above, the E220R- and LE220R-plus are designed to be integrated

with a sample handling robot in an automated work cell. The front door of the safety enclosure contains a cutout providing the robot

arm access to the inside of the instrument cabinet. The instrument presents the sample tray at the opening, and the robot arm loads

or unloads the sample through the opening. The instrument is controlled by custom soware built using the SonoLab 10 API Toolkit.

A light curtain has been added as an addional safety interlock. The light curtain is wired into a safety rated relay controller, the output

of which is connected to the Covaris controller board. If an operator reaches through the robot access opening while the system is

running, the motor power and acouscs are immediately disabled.

The light curtain consists of invisible, infrared light and an infrared detector. Beams of light on 14 mm spacing are transmied between

the source and detector. Blocking one or more beams of light will trigger the safety interlock system. Acouscs and moon will remain

paused unl the beams are unobstructed.

1.4 The LE220Rsc Focused-ultrasonicator

The LE220Rsc is an enhanced version of the LE220R-plus. In addion to the features of the LE220-plus and LE220R-plus, the LE220Rsc

contains an extended travel X axis moon stage. This longer travel stage allows treatment of 96 and 384 posion well plates using the

Covaris scanning treatment method. The scanning treatment method allows for connual AFA-treatment as the plate passes over the

transducer, without pausing between columns. The scanning speed and number of iteraons determines the total acousc dose.

1. Introducon

CAUTION: Equipment damage may occur.WARNING: Personal injury may occur.NOTE: Inconvenience if disregarded.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

2.1 Assembly

The following items should be found in the crate containing the instrument:

• Main unit

• Notebook computer with AC adapter

• Cables

- USB cable

- Instrument power cord

- Computer AC adapter power cord

• Accessories

- AFA ultraCUBE, consisng of:

○ Water Condioning System 2.0 (WCS)

○ CH05 Chiller

- Chiller hose kit

- Sample tubes & racks (varies by applicaon)

- Water bath (installed in main unit)

- AutoFill accessory kit

Unpack the instrument, the Water Condioning System 2.0 (WCS), and the CH05 chiller. Refer to the WCS User Manual to install

the UV lamp into the WCS. The lamp is shipped separately to prevent breakage during shipment. Arrange the instrument, WCS, and

chiller on a bench as shown. The chiller and WCS may be installed on either side of the system. The CH05 and WCS may also be

placed on the oor or such that the hoses may reach the front of the water bath of the water bath.

2. Installaon and Setup

E220R-plus Focused-ultrasonicator

CAUTION: When posioning the system, please keep the fan and rear panel vents free of obstrucons which can block the

ow of air.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

• Connect the hoses between the WCS and the CH05 as shown above.

• For the E220-, E220R-, LE220-, and LE220R-plus, connect the remaining two hoses between the two ports on your instrument

water bath and the remaining two ports on the opposite side of the WCS. Connect the lower hose to the ng on the le side

of the water bath.

For the LE220Rsc, the upper hose from the WCS 2.0 must connect to the lower ng of the water bath. The temperature

sensor for the water bath is adjacent to the two hose connecons. The lower hose ng contains a long tube to bring the

cooled (heated) water from the chiller away from the temperature sensor to prevent an erroneous temperature reading.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

The WCS contains a parculate lter and UV lamp to prevent biological growth. This allows the water bath to be changed only

once per month. Refer to the User Maintenance Secon of this manual for addional details.

• The AutoFill opon allows plant deionized water to be plumbed directly to the instrument to allow automac lling of the water

bath without any operator intervenon. If the AutoFill opon is going to be used, refer to the instrucons included with the

AutoFill accessory kit or Appendix D of this manual to assemble the components of the AutoFill Accessory Kit, and connect the

plant water to the system.

• Plug the instrument into a power source using the enclosed line cord. The instrument is a “universal input” device and

automacally employs any worldwide line voltage or frequency (100 to 240 volts AC, 50 or 60 Hz). Leave the instrument power

and notebook power switched OFF during this me.

• Plug one end of the USB cable into the instrument USB Port and the other end into any USB port on your notebook computer.

Connect the temperature control cable between the D-connector at the rear of the instrument and the D-connector on the

CH05 chiller.

• Connect the CH05 to a power source. The CH05 is a universal input device. Do not turn on the CH05 at this me.

• Locate the WCS power supply and line cord. Connect the WCS power supply to the port provided on the WCS and connect the

line cord to a power source. The power supply is a universal input device. Leave the WCS turned o at this me.

• Connect the computer to a power source. Connect the mouse or other accessories to the computer.

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instrucon Manual Part Number: 010521 Rev B | May 2021

• Refer to the rear of the instrument’s right-side panel to idenfy the following features:

• Refer to the gure below to idenfy the following items:

• Power on the instrument. The power switch is located at the right rear, near the power entry module.

• Press and hold the green ‘Door’ buon while pulling the door handle to open the safety enclosure front door. Slide out the

water bath and remove it from its plasc shipping bag. Remove and discard the desiccant pack.

The instrument is now ready to be started.

The Rear Right Panel of the System

USB Port

AC Line (Mains) Input

and Power Switch

Product Safety Label

Serial Tag, Regulatory, and Line

Power Label

E220R-plus LE220-plus LE220Rsc

Enclosure Door

Safety Lock

Door Lock

Release Buon

Stop

Buon

Safety

Enclosure

Water

Bath

LE220Rsc with extended bath

and ngs on the le side of bath

This manual suits for next models

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