Covaris LE220 Manual

Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
Part Number: 010398 Rev H | November 2019
LE220 (-plus, R-plus, and Rsc)
Setup & Instruction Manual
Instrument and Software Developed for High-performance Sample
Preparation using Adaptive Focused Acoustic
®
(AFA
®
) Energy
For Research Only
Model Numbers: LE220-plus, LE220R-plus, and LE220Rsc | Software: SonoLab 8

Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
Trademarks
Patented or patent pending and registered or registration-pending trademarks of Covaris are protected.
Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected
by law.
WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries. Teflon is a
registered trademark of The DuPont Company.
Information subject to change without notice
For Research Use Only (RUO)
Not for use in Diagnostic Procedures Publication
PN 010398
Revision H, November 2019
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applications 2019©
Covaris Inc., All Rights Reserved
Covaris, Inc.
14 Gill St, Unit H Woburn,
Massachusetts 01801-
1721 USA
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to
contain an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts
of the following government and clinical standards, or their equivalent in the country of use:
•Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other Bloodborne
Infections, published 1987, updated 1996
•Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline - Second
Edition, published 2004, ISBN 1-56238-530-5
•Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from Occupationally
Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674
•Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
•International Standards Organization (ISO) 15190:2003 Medical Laboratories – Requirements for Safety

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
Warnings
For the Safety of Operating Personnel:
•Make sure that the equipment is properly grounded. DO NOT operate if it’s not properly grounded.
•The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any circumstances, remove the grounding
prong from the power cord.
•DO NOT run an acoustic treatment with the sample cover in the OPEN position or without a water bath - the acoustic system will not
work.
•If there is any indication that the Safety System is not functioning properly, DO NOT operate the equipment and contact Covaris
immediately.
•If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
To Prevent Damage to the Equipment:
•The instruments are designed to operate in ambient laboratory conditions e.g., 19 to 25 °C (66 to 77 °F).
DO NOT operate the instrument in a cold room environment. The system is designed to operate with a water bath and re- circulating
heater/chiller apparatus to control sample temperature.
•NEVER run a method without water in the bath since this could damage the transducer. The instrument is equipped with a water level
sensor to protect the transducer and degassing pump. The system will not allow the degassing pump to operate or the acoustic
treatment to start unless an adequate volume of water is detected.
•Distilled or deionized water should be used to fill the water bath.
•Unless a Water Conditioning System 3.0 (WCS) is actively employed, empty the water bath and wipe it dry EVERY DAY with a lint-free
cloth. DO NOT leave water in the tank for an extended time as there is no water filtration or water cleaning system within the apparatus
(unless WCS is employed).
•DO NOT employ isopropyl alcohol, ammonia-based or abrasive cleaners in the system, as these will damage the acrylic surfaces.
•Establish a standard of operation and test the equipment periodically, as described in the maintenance section of this manual.
CAUTION: All WCS 3 components have pressure ratings above Covaris’ specified maximum operating pressure
which have been extensively tested. However, if plant DI water is connected to the WCS, a catastrophic failure
may result in substantial plant water spillage. It is the user’s responsibility to specify and implement appropriate
containment/alarm schema for their facility.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
Warranty
When used in accordance with written instruction and under normal operating conditions, the Covaris instruments are guaranteed to be
free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original delivery by an authorized representative. Any
component proven defective during the stated period will be repaired free of charge or replaced at the sole
discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A. provided that the defective component is returned properly packaged with all
transportation charges prepaid. The customer is expected to perform basic diagnostics and component replacement via telephone support from
Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the customer. A limited
warranty as specified may apply to certain components of the equipment.
Warranty Exceptions
This warranty is void if failure of the software or hardware has resulted from accidents, abuse, improper maintenance or repair, or by
misapplication from the customer. It is also void if damage is caused by any unauthorized attachments or if modifications are made to the
equipment. Removing or tampering with the Safety Enclosure will void the warranty, and the customer will assume all liabilities.
This warranty is limited to the original purchaser and is not transferable.
The software will perform according to the accompanying written materials and the medium on which the software is delivered is free of
defects in materials under normal use and service. The warranty is void if damage has resulted from third party software not intended for
use with the system.
The high-power focused transducer is designed to give maximal mechanical energy output in water. Permanent damage to the transducer and
electronic circuits could result if the transducer is operated without water. Operation of the system without water in the water bath voids the
warranty.
CONTACT COVARIS, INC. SHOULD YOU HAVE QUESTIONS OR CONCERNS PERTAINING TO THE USE OF OUR EQUIPMENT.
Warranty Services
The purchased equipment is covered by a twelve (12) month warranty which includes all the necessary services and support necessary so that
the customer can successfully operate their equipment. Extended warranties are available at the end of the original 12 month warranty period.
Services included with the original purchase of the system are:
Technical Support – Ongoing assistance with the operation or application of the equipment and/or troubleshooting is provided via:
•Telephone
-United States: Tel: [phone] during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Eastern
Standard Time (EST), Greenwich Mean Time (GMT-05:00)
-Europe: Tel: [phone], during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
•E-mail queries to techsuppo[email protected] or [e-mail]
Parts Replacement – Replacement of parts (excluding consumables) from normal operation of equipment are provided on a priority basis.
All labor and shipping charges are included. Failure due to accident, abuse, or improper operation is not covered.
This manual includes maintenance guidelines and troubleshooting tips.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
Table of Contents
1. Introduction .................................................................................................................................................................................... 6
1.1 Overview of the Instruction Manual ....................................................................................................................................6
1.2 The LE220-plus Instrument................................................................................................................................................... 6
1.3 The LE220R-plus Instrument ................................................................................................................................................6
1.4 The LE220Rsc Instrument .....................................................................................................................................................6
2. Installation and Setup ..................................................................................................................................................................... 7
2.1 Assembly............................................................................................................................................................................... 7
3. Getting Started................................................................................................................................................................................ 11
3.1 Fill the Water Bath................................................................................................................................................................ 11
3.2 Start the System ................................................................................................................................................................... 11
3.3 Preparing your System for Use ............................................................................................................................................. 13
3.4 Running a Method................................................................................................................................................................ 22
3.5 Daily System Shutdown ........................................................................................................................................................ 23
4. Create and Edit Methods ................................................................................................................................................................ 24
4.1 Create New Method ............................................................................................................................................................. 24
4.2 Creating Methods using Repeat Function ............................................................................................................................ 30
4.3 Scanning Treatments (LE220Rsc Only) ................................................................................................................................. 33
4.4 Modifying Existing Methods................................................................................................................................................. 34
5. SonoLab™ Application Software ..................................................................................................................................................... 35
5.1 Home Screen ........................................................................................................................................................................ 35
5.2 History Tab ................................................................................................................................................................................................ 35
5.3 About Tab .................................................................................................................................................................................................. 37
5.4 Admin Tab ................................................................................................................................................................................................. 38
6. System Specifications...................................................................................................................................................................... 42
7. Maintenance ................................................................................................................................................................................... 43
7.1 Cooling Air Intake ................................................................................................................................................................. 43
7.2 Water Bath ........................................................................................................................................................................... 43
7.3 Transducer............................................................................................................................................................................ 44
7.4 Safety System ....................................................................................................................................................................... 44
7.5 Degassing System ................................................................................................................................................................. 44
7.6 Cleaning the System ............................................................................................................................................................. 44
7.7 System Shutdown................................................................................................................................................................. 44
8. Troubleshooting .............................................................................................................................................................................. 46
9. Appendix ......................................................................................................................................................................................... 48
Appendix A: LE220R-plus (LE220-plus) Series Site or Transport Preparation ............................................................................. 48
Appendix B: Installing SonoLab 8 and Required Support Software ............................................................................................ 49
Appendix C: Humidity and Water Management for the Covaris LE220-plus .............................................................................. 54
Appendix D: Connecting AutoFill to Plant Water ........................................................................................................................ 56
Addendum for LE220Rsc...................................................................................................................................................................... 57

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
1. Introduction
1.1 Overview of the User Manual
This manual contains operation and maintenance instructions for the Covaris LE220-plus, LE220R-plus, and LE220Rsc Focused- ultrasonicators. This
document contains information essential to the proper use and care ,of your equipment. Should any unforeseen problems occur with the normal
operation of the equipment, contact Covaris Technical Support immediately.
The following definitions apply in this manual:
NOTE: Inconvenience if disregarded. WARNING: Personal injury may occur. CAUTION: Equipment damage may occur.
There is also a PDF version of this manual, typically available on the computer desktop. Open the manual by double clicking the user manual
icon.
1.2 The LE220-plus Instrument
The LE220-plus Focused-ultrasonicators are based on Covaris Adaptive Focused Acoustics (AFA) technology. These instruments are designed to
provide researchers an integrated stand-alone tool to be used in a laboratory environment. The instrument is ideal for those who work with
tissue homogenization, cell lysis, DNA or chromatin shearing, and other sample preparation steps.
The system is comprised of the:
•Treatment System which delivers the acoustic energy to the sample
•Safety System which protects users from inadvertently contacting the high intensity acoustic energy
•Computer and Application Software which provides an interface to control the device via the API
•Water Management System; which consists of a cooler/heater and a UV treatment chamber with particulate filter to maintain water
quality in the instrument bath
1.3 The LE220R-plus Instrument
In addition to the features of the LE220-plus described above, the LE220R-plus is designed to be integrated with a sample handling robot in an
automated work cell. The front door of the safety enclosure contains a cutout providing the robot arm access to the inside of the LE220R-plus
cabinet. The LE220R-plus presents the sample tray at the opening, and the robot arm loads or unloads the sample through the opening. The
LE220R-plus is controlled by custom software built using the Covaris Integration Toolkit.
A light curtain has been added as an additional safety interlock. The light curtain is wired into a safety rated relay controller the output of
which is connected to the Covaris controller board. If an operator reaches through the robot access opening while the system is running, motor
power and acoustics are immediately disabled.
The light curtain consists of invisible, infrared light and an infrared detector. Beams of light on 14 mm spacing are transmitted between the
source and detector. Blocking one or more beams of light trigger the safety interlock system. Acoustics and motion will remain paused until
the beams are unobstructed.
1.4 The LE220Rsc Instrument
The LE220Rsc is an enhanced version of the LE220R-plus. In addition to the features of the LE220-plus and LE220R-plus, the LE220Rsc
contains an extended travel X axis motion stage. This longer travel stage allows treatment of 96 and 384 position well plates using the
Covaris scanning treatment method.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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2. Installation and Setup
2.1 Assembly
The following items should be found in the crate containing the instrument:
•Main unit
•Notebook computer with AC adapter
•Cables
-USB cable
-Instrument power cord
-Computer AC adapter power cord
•Accessories
-AFA ultraCUBE, consisting of:
○Water Conditioning System 2.0 (WCS)
○CH05 Chiller
-Chiller hose kit
-Sample tubes & racks (varies by application)
-Water tank (installed in main unit)
-AutoFill accessory kit
LE220-plus Focused-ultrasonicator LE220R-plus Focused-ultrasonicator
2.2.1 Unpack the instrument, the Water Conditioning System 2.0 (WCS), and the CH05 chiller. Refer to the WCS User Manual to install
the quartz tube and UV lamp into the WCS. The quartz tube and lamp are shipped separately to prevent breakage during shipment.
Arrange the instrument, WCS, and chiller on a bench as shown below. The chiller and WCS may be installed on either side of the
system. The CH05 and WCS may also be placed on the floor, if they can be located such that the hoses reach the front of the water
bath.
CAUTION: When positioning the system, please keep the fan and rear panel vents free of obstructions that may block
the flow of air.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Connect the hoses between the WCS and the CH05 as shown above.
Connect the remaining two hoses between the two ports on your LE220R-plus or LE220-plus water bath and the remaining two ports on the
opposite side of the WCS. It doesn’t matter which bath port connects to which WCS port for these two hoses.
The WCS contains a particulate filter and UV lamp to prevent biological growth. This allows the water bath to be changed just once per
month. Refer to the User Maintenance section of this manual for details.
NOTE: See the addendum at the end of the manual for connecting the hoses to the LE220Rsc water bath. The
connections are not interchangeable on the LE220Rsc.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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2.2.2 The AutoFill option allows plant deionized water to be plumbed directly to the LE220R-plus and LE220-plus to allow automatic
filling of the water bath without any operator intervention. If the AutoFill option is going to be used, refer to the instructions
included with the AutoFill accessory kit or Appendix D of this manual to assemble the components of the AutoFill Accessory Kit, and
connect plant water to the system.
2.2.3 Plug the instrument into a power source using the enclosed line cord. The instrument is a “universal input” device and automatically
employs any worldwide line voltage or frequency (100 to 240 volts AC, 50 or 60 Hz). Leave the instrument power and notebook
power switched OFF at this time.
2.2.4 Plug one end of the USB cable into the instrument USB Port and the other end into any USB port on the notebook computer.
2.2.5 Connect the temperature control cable between the D-connector at the rear of the LE220-plus or LE220R-plus and the D-
connector on the CH05 chiller.
2.2.6 Connect the CH05 to a power source. The CH05 is a universal input device. Do not turn on the CH05 at this time.
2.2.7 Locate the WCS power supply and line cord. Connect the WCS power supply to the port provided on the WCS and connect the line
cord to a power source. The power supply is a universal input device. Leave the WCS turned off at this time.
2.2.8 Connect the computer to a power source. Connect the mouse or other accessories to the computer.
2.2.9 Refer to the rear of the instrument’s right-side panel and identify the following features as necessary:
The Rear Right Panel of the System
AC Line (Mains) Input
and Power Switch
USB Port
Serial Tag,
Regulatory, and
Line Power Label
Product Safety Label

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2.2.10 Refer to the figure below and identify the following items:
LE220R-plus LE220-plus
2.2.11 Power on the instrument using the power switch at the right rear, at the power entry module.
2.2.12 Press and hold the green ‘Door’ button while pulling the door handle to open the safety enclosure front door. Slide out the water tank
and remove it from its plastic shipping bag. Remove and discard the desiccant pack.
The instrument is now ready to be started.
Enclosure Door
Safety Lock
Enclosure
Door Handle
Door Lock
Release Button
Stop Button
Safety Enclosure
Water Tank

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3. Getting Started
3.1 Fill the Water Bath
The instrument’s transducer must be immersed in a water bath to transmit high-frequency, focused
acoustic energy to the sample tube. For best performance, employ only clean, distilled or deionized
water in the bath.
Open the front door, remove the tank and the first time your system is operated, fill the bath initially
about half full. The first time the WCS and chiller is operated, a substantial amount of air is forced
from the WCS into the bath, which may cause a full bath to overflow. This only occurs the first time an
empty WCS is used.
Once the WCS is filled with water, the bath should be filled to a level of 15. If the transducer is in the
raised position, out of the bath, fill to 15 on the “Fill” side of the gauge. If the transducer is submerged,
fill to 15 on the “Run” side of the gauge.
If the AutoFill option is enabled, after the initial filling of the bath, AutoFill will maintain a sufficient water level in the bath
automatically.
3.2 Start the System
Turn the instrument power ON using the power switch located on the rear, right side of the instrument at the
power entry module. When power is ON, the green door-lock release switch will illuminate and the ventilation
fans in the rear panels will run.
Power on the WCS and CH05 chiller. SonoLab software will perform its temperature control; it is not necessary
to adjust temperature set points from the CH05 control panel.
The first time the chiller and WCS are operated, the WCS filter and UV treatment chamber are empty, and this air is purged as described
above. This is normal, and only occurs the first time the chiller/WCS assembly are operated.
Start the system computer. The computer employs a Windows 10 (or later) Operating System.
SonoLab is an application used to control a Covaris Focused-ultrasonicator for processing a biological or chemical sample. It is designed to
create, edit, and run a processing program called a method. It has provisions to run, pause or stop a method and view execution histories
which contain a record of key instrument parameter values during a run. It also contains editors for customizing system settings and
performing maintenance functions.
SonoLab is installed on the computer purchased with the instrument. No further installation or configuration is required.
Find the SonoLab icon on the desktop:
If the icon is not visible, or if SonoLab is not installed, please refer to Appendix B or contact the Covaris Technical Support for installation
instructions.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Double-click on the SonoLab icon to start SonoLab; the splash screen will appear:
Following the splash screen, the SonoLab Home Screen will appear with the login dialog box in front of it.
SonoLab is preconfigured with a single administrative user named “Administrator” whose password is “admin”. User Authentication and creating
new users and their permissions are described in Section 5.4, the ADMIN Tab.
Enter the username and password and click ‘LOGIN’
Following login, the motion system homing dialog appears:
Click ‘Yes’. The transducer and sample holder mechanical assemblies will move into position and the SonoLab Home Screen will display.

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LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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If SonoLab does not connect to the instrument, the following screen appears allowing SonoLab to be run in emulation mode without an
instrument.
Click “Yes”, and the normal SonoLab login and Homing screens are presented, allowing the user to operate SonoLab as if an instrument
is present. This is useful for training purposes or for creating methods off-line.
3.3 Preparing your System for Use
3.3.1 Instrument Status & Basic Controls
To run an acoustic treatment, a number of conditions must be met, including:
a) The motion system must be initialized.
b)There must be an acoustic method selected in the Method box.
c) Water temperature must be within acceptable Min and Max limits.
d)The bath water must be degassed.
e) Water should be approximately at level 15.
f) The safety enclosure door must be closed.
NOTE: The proper plate and consumables must be in place on your system’s sample tray.

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The “Run” button will remain gray and inactive until a method is selected and all necessary conditions are met. Your system automatically
sets the temperature when a method is selected. After a specific user’s initial log in, SonoLab will automatically select the last method run
by this user the next time they log in. The bath water temperature will then be set according to this method.
To achieve an acceptable status on the other conditions:
a) Your system’s motion system normally initializes when SonoLab starts, unless the user purposely skipped this step. In this case, the
motion system can be homed from the Motion Control pane.
Click on the Motion Control button to display the Motion Control pane.
Click on “Home” to initialize the motion system. See additional information for the Motion Control pane in this section of the manual
under ‘Other Controls on the Home Screen’.
b)To select and load an acoustic method, click on the panel displaying the currently loaded method, as shown below, and a list of
existing method names will be displayed in a drop-down list. Navigate through the list with the vertical scroll bar and select a
method.
The method’s run time (minutes and seconds) and temperature set point will be displayed in the lower right corner of the main
screen as shown.
Drop-down list of available
Currently loaded method

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c) An important principal of the Covaris Focused-ultrasonicator is isothermal temperature control. Your system automatically adjusts the
water temperature, as necessary, to match the method temperature set point once a method is selected. Water Temperature status
will turn green when temperature is within the Min and Max limits (by default 5 degrees above or below the set point). The default
set point (in the absence of a selected method) is 20 ºC.
Bath cool down from room temperature to below 10 degrees takes about 30 minutes. Once a method starts, if the
bath temperature rises beyond the user specified limit, your system finishes the current row being treated, pauses treatment, and
automatically resumes when the temperature is back within limits, with no operator intervention required.
d)Properly degassed water is important for efficient transfer of acoustic energy from the instrument’s transducer to the sample being
treated. If water is detected in the bath, the degassing pump is automatically turned on when SonoLab is started. The pump should
be allowed to run until the Degas indicator on the Status Screen turns green (60 minutes) prior to an acoustic treatment. The degas
pump can be manually turned on and off from the Water Works pane. See additional information below in section Other Controls on
the Home Screen.
e) When the “Run” button is clicked to start the method, SonoLab checks that the bath water is at an appropriate level for the
consumables employed in the method. If the water level is incorrect, the method is paused and a message to adjust the water level
is presented.
The remaining conditions, along with their related status, are displayed in the Instrument Status panel. Click the “System Status” button
to display the Instrument Status panel. An acceptable condition is indicated by a green check mark in the Status box.
If a condition is not acceptable, the status will show X.
If any of the items other than “Water Level” and “Temperature within Limits” in the Status list display X, a red X will also be displayed in
the System Status button, as shown below. Water level and temperature will be adjusted as necessary once a specific method is started.

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A checkbox is provided at the bottom of the status screen to override the degas timer and temperature limits to allow your system to run a
method without waiting for these conditions to be met. With the temperature and degas override boxes checked, the display changes to
yellow caution indicators as shown.
Once a method has been loaded and all conditions are acceptable, the ‘Run’ button will turn green and the selected acoustic Method
can be started.
To modify an existing method, see Section 5 Create and Edit Methods.
A red X in any items in
the Status list results in
a red X in the System
Status button

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3.3.2 Other Controls on the HOME Screen
•Lightbulb: This is both a control and indicator. The enclosure light is off when SonoLab
starts. Clicking on the light bulb image will turn the light ON, clicking again will turn it
OFF.
•Barcode Scanning: Your system allows barcoded samples to be scanned prior to treatment. The barcode data is then
associated with the specific method run and stored in the both the .csv and .xhst history files found in
Public Documents\Covaris\SonoLab 8\History.
Plates, in which all tubes are integrated into a single assembly, allow a single barcode entry for the entire plate. Racks allow
separate entries for each row to be treated by the method.
When the barcode scanning button is switched on, the green “Run” button changes to a yellow “Scan” button.
Click the “Scan” button, then click “Yes” when prompted to confirm scanning. The
“Scan/Enter Barcode” box displays. Scan the barcode, or, optionally, enter a value or label
using the computer keyboard.
Click “Next Position”. If a plate is being treated, the “Scan/Enter” box closes and the “Scan”
button changes to a green “Run” button.
If a rack is being treated, clicking the “Next Position” button provides an entry field for
the next row to be treated. Enter its barcode and click “Next Position”. Repeat until all
rows to be treated have been scanned. After the last row has been scanned, the entry
window closes, and the yellow “Scan” button becomes a green “Run” button, as
described above.
Barcode Scanning
Turned “On”
“Run” Button
Changes to “Scan”

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•RFID: Beginning in SonoLab version 8.4, RFID scanning is implemented. When a method employing the Covaris AFA-TUBE® is started,
the sample tray is passed near an RFID reader mounted to the back of the water bath. An encrypted chip embedded in the AFA-
TUBES’s barcode label is read. The AFA-TUBE’s serial number, date of manufacture, and proof of authenticity is stored in the LIMS
folder. Methods employing the Covaris AFA-TUBE will not execute if the barcode label is not present.
•Water Works Button: Water Works allows the user to control the chiller and degas pump either manually or by automatic scheduling. For
example, the schedule can be set to begin the degassing and chilling early in the morning so that your system will be ready to use when
personnel arrive. Click on the “Water Works” button at the lower right of the Home screen to display the Water Works pane.
The Water Works pane contains the following:
-ON/OFF switches for chiller and degas pump: these are both switches and indicators. The current status is highlighted in blue, as
shown above. These switches are disabled when operating in Auto (scheduled) Mode.
-Current chiller set point, current system bath temperature, and current system bath water level.
-Enable/disable switch for automatic temperature and degas pump control: this is both a switch and indicator. It toggles between
Auto Mode, as shown above, and “Auto is Off” when automatic control is not active. In Auto Mode, the chiller, chiller temperature
set point, and degas pump are controlled by the user defined schedule.
-Enable/disable Standby Mode button: this is both a switch and indicator. It toggles between “Click for Standby Mode” and Standby
Mode. In Standby Mode, the chiller cooler/heater element is disabled, and the transducer is raised out of the water bath.
Scheduled temperature control and degassing are disabled when the instrument is in Standby Mode.
-Fill Tank button (optional): if the AutoFill option for the instrument bath is enabled on the
system, this button will be present in the Water Works pane. Clicking the button fills the
bath. Depending on the amount of water added, a degassing delay will occur, during which
methods cannot be run. The following table summarizes the degassing delay time.

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• Automatic Water Level Control (AutoFill): The AutoFill option consists of hardware to implement connection of plant deionized
water to your system so that sufficient bath water is automatically maintained to allow instrument operation. Refer to the appendix
of this manual for instructions on making connections to AutoFill. AutoFill is enabled by an entry in a configuration file. The water
level is checked every 15 minutes when the instrument is idle, and before each method is run. If necessary, water should be added
to the bath followed by a delay to allow degassing of the added water to be executed. Degassing time is determined according to the
table below.
• Automatic Chiller/Degas Operation: An automatic schedule for degassing and chilling your system’s water bath is a convenient
way to have the instrument ready to use at the beginning of the day. Under software control, your system’s water bath will
automatically activate degassing and adjust to the user specified temperature at the beginning of the schedule period. It will
also turn off the degas pump and place the chiller in standby mode at the end of the scheduled period. In standby mode, the
chiller’s heating/cooling circuit is disabled while the pump continues to operate, circulating
bath water through the WCS filter. Refer to the ultraCUBE User Manual for details about the operation of the chiller and WCS.
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To configure a schedule, perform the following steps:
-Double click anywhere in table shown in the image above to display the Automations Settings window.
Note: To configure a schedule for just one specific day, Sunday through Monday, double-click on that day in the table.
NOTE: All accounts must be logged out of SonoLab for the automatic temperature control operation. At
the time scheduled for cooling to turn on or off, any logged in user’s method will override the automatic
settings. The automatic settings will not then immediately take effect when this user logs out. The
automatic settings will only take effect at the next scheduled change.

Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
20
Part Number: 010398 Rev H | November 2019
-Click in the check boxes for the functions to be scheduled. Normally, all four boxes will be checked.
-Click on the clock icon for Degas on, circled above. This displays a clock.
-Click on either AM or PM, then on the hour the degas pump is to start. The clock changes to display the hour selected (7 o’clock
in this example) and the minutes part of the time highlighted.