Covaris M Series User manual

For research use only
M-Series
SERVICE MANUAL

M220 Service Manual Page 2 410085 Rev. B
Trademarks
Patented or patent pending and registered or registration-pending are trademarks of Covaris.
Registered names and trademarks used in this document, even when not specifically marked as such, are
not to be considered unprotected by law.
Information subject to change without notice
For Research Use Only
Not for use in diagnostic procedures
Publication P/N 410085
September 2013
Product covered by Patent No. US6, 719,449, US6, 948,843, and other pending applications
Copyright © 2013 Covaris, all rights reserved.
Covaris, Inc.
14 Gill St, Unit H
Woburn, Massachusetts
01801-1721 USA Printed in U.S.A.
UNIVERSAL PRECAUTIONS
Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain
an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following
governmental and clinical standards, or their equivalent in the country of use:
•Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other
Bloodborne Infections, published 1987, updated 1996
•Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline -
Second Edition, published 2004, ISBN 1-56238-530-5
•Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from
Occupationally Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674
•Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens
•International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety

M220 Service Manual Page 3 410085 Rev. B
Warnings
For safety of operating personnel:
Make sure that the equipment is properly grounded. DO NOT operate if it is not properly grounded.
The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any
circumstances, remove the grounding prong from the power cord.
DO NOT attempt to operate the equipment with the Acoustic Assembly and sample cover in the
open position the acoustic system will not work. If there is any indication that the Safety System is
not functioning properly, DO NOT operate the equipment and contact Covaris immediately.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by
the equipment may be impaired.
To prevent damage to the equipment:
The instruments are designed to operate in ambient laboratory conditions e.g., 15 to 32°C (60 to
90°F). DO NOT operate the instrument in a cold room environment; the system is designed to
operate with a water bath and heater/chiller apparatus to control sample temperature.
NEVER run a method without a water bath; this could damage the transducer. The instrument is
equipped with a water level sensor to protect the transducer. The system will not allow the acoustic
wave treatment to start unless an adequate volume of water is detected.
Covaris AFA water should be used to fill the water bath.
Empty the water bath and wipe it dry EVERY day with a lint-free cloth. DO NOT leave water in the
instrument for an extended time as there is no water filtration or water cleaning system with the
apparatus.
Do not employ isopropyl alcohol, ammonia-based or abrasive cleaners on the instrument, as these
will damage the acrylic surfaces.
DO NOT load third party software onto the system without consulting with Covaris.

M220 Service Manual Page 4 410085 Rev. B
1). Scope of This Manual................................................................................................... 6
2). Safety and EMC Compliance Information ................................................................... 6
2.1 General Instrument Safety.................................................................................... 6
2.1.1 Acoustic Safety............................................................................................. 6
2.1.2 Safety and Electromagnetic Compatibility (EMC) Standards...................... 6
3). Introduction................................................................................................................... 7
Transducer....................................................................................................................... 7
Interlock Subsystem........................................................................................................ 7
Control Electronics System............................................................................................. 7
4). Maintenance and Repair ............................................................................................... 8
Recommended Daily Maintenance................................................................................. 8
Troubleshooting.............................................................................................................. 8
Diagnosis......................................................................................................................... 9
Contamination............................................................................................................. 9
Bath Temperature........................................................................................................ 9
Position of Treatment Vessel...................................................................................... 9
Water Level Errors...................................................................................................... 9
Acoustic Power Issues .............................................................................................. 10
Interlock Errors......................................................................................................... 10
Communication Errors.............................................................................................. 10
Other Indicators ........................................................................................................ 10
Repair Procedures......................................................................................................... 11
General Procedures................................................................................................... 11
Removing the outer Covers ...................................................................................... 11
Replacing the Water Level & Temperature Sensor Assembly................................. 16
Diagnosing and Replacing Printed Circuit Boards:...................................................... 25
RF Power Board........................................................................................................ 26
Controller Board:...................................................................................................... 29
Thermo-Electric (TE) Controller Board ................................................................... 33
Capacitor Reserve Board .......................................................................................... 35
Power Supplies.......................................................................................................... 38
24 Volt Supply.......................................................................................................... 38
48 Volt Supply.......................................................................................................... 38
Acoustic Power Testing Procedure................................................................................... 43

M220 Service Manual Page 5 410085 Rev. B
Measure Power.............................................................................................................. 43
5Tools and Fixtures......................................................................................................... 43
6). M220 Series: SonoLab 7............................................................................................. 43
7). Appendix A................................................................................................................. 45
8). System Wiring Diagram ............................................................................................. 46