cytiva Chromaflow 50 User manual

Chromaflow Packing Station
50, 100, 200, and 400
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 4
1.1 About this manual ......................................................................................................................................... 5
1.2 Important user information ....................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 8
2 Safety instructions ............................................................................................. 9
2.1 Safety precautions ........................................................................................................................................ 10
2.2 Labels ................................................................................................................................................................. 16
2.3 Emergency procedures ............................................................................................................................... 18
3 System description ............................................................................................ 22
3.1 Chromaflow Packing Station main components .............................................................................. 23
3.2 Control panels ................................................................................................................................................. 27
3.3 Flowchart .......................................................................................................................................................... 33
4 Installation .......................................................................................................... 36
4.1 Site requirements .......................................................................................................................................... 38
4.2 Transport in crate .......................................................................................................................................... 39
4.3 Unpack from the crate ................................................................................................................................. 40
4.4 Transport when unpacked .......................................................................................................................... 45
4.5 Setup ................................................................................................................................................................... 46
4.5.1 Set up your Chromaflow Packing Station ......................................................................................... 47
4.5.2 Connect compressed air supply ............................................................................................................. 48
4.5.3 Guidelines for connections ....................................................................................................................... 49
4.5.4 Grounding connection ................................................................................................................................ 50
4.5.5 Connect a column ........................................................................................................................................ 51
5 Operation ............................................................................................................ 56
5.1 Prepare your Chromaflow Packing Station ........................................................................................ 58
5.1.1 Final actions before operating ................................................................................................................ 59
5.1.2 Priming .............................................................................................................................................................. 60
5.2 Pack a column ................................................................................................................................................. 61
5.3 Unpack a column ............................................................................................................................................ 62
5.4 Disconnection after use .............................................................................................................................. 63
6 Maintenance ....................................................................................................... 64
6.1 User maintenance schedule ...................................................................................................................... 66
6.2 Cleaning ............................................................................................................................................................ 68
6.2.1 Cleaning a Chromaflow Packing Station without a connected column ............................... 70
6.2.2 Cleaning a Chromaflow Packing Station and a connected column ....................................... 75
6.3 Storage .............................................................................................................................................................. 76
7 Troubleshooting ................................................................................................. 78
8 Reference information ....................................................................................... 80
8.1 Specifications .................................................................................................................................................. 81
8.2 Chemical resistance ..................................................................................................................................... 85
Table of Contents
2 Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD

8.3 Noise level ......................................................................................................................................................... 86
8.4 Recycling information .................................................................................................................................. 87
8.5 Regulatory information ............................................................................................................................... 88
8.5.1 Contact information .................................................................................................................................... 89
8.5.2 European Union and European Economic Area .............................................................................. 90
8.5.3 Other regulations and standards .......................................................................................................... 91
8.6 Health and Safety Declaration Form ...................................................................................................... 92
8.7 Further information ...................................................................................................................................... 94
Index ........................................................................................................................... 95
Table of Contents
Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD 3

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, intended use of Chromaflow™ Packing Station, and a list of associ-
ated documentation.
In this chapter
Section See page
1.1 About this manual 5
1.2 Important user information 6
1.3 Associated documentation 8
1 Introduction
4 Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate,
and maintain the product in a safe way.
Scope of this document
The Operating Instructions cover all sizes of Chromaflow Packing Station.
For detailed information about each individual Chromaflow Packing Station refer to
the relevant information in the documentation package provided with each product.
Contact your local Cytiva representative for more information, if needed.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD 5

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use
Chromaflow Packing Station is intended to be used for packing and unpacking
Chromaflow columns with chromatography resin slurry.
Chromaflow Packing Station shall not be used in any clinical procedures, or for diag-
nostic purposes.
Chromaflow Packing Station is not suitable for operation in a potentially explosive
atmosphere or for handling liquids under conditions at which they could be flammable.
Prerequisites
In order to operate your Chromaflow Packing Station safely and according to the
intended purpose, the following prerequisites must be met:
•You should be acquainted with the use of general bioprocessing equipment and
with the handling of biological materials.
•You must read and understand the Safety chapter of these Operating Instructions.
•The system must be installed according to the instructions in Chapter 4 Installation,
on page 36.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
1 Introduction
1.2 Important user information
6 Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD

WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD 7

1.3 Associated documentation
Introduction
This section describes the documentation associated with your Chromaflow Packing
Station.
Product specific documentation
In addition to the Operating Instructions manual, the documentation package supplied
with each Chromaflow Packing Station also includes detailed specifications and trace-
ability documents.
The most important documents in the documentation package with regard to tech-
nical aspects of each Chromaflow Packing Station are:
Document Abbrevia-
tion
Contents
Piping and Instrumenta-
tion Diagram
P&ID Schematic overview of the entire
process flow, including all components
and instruments.
General Specification GS Technical data for the system.
Assembly Drawing AD Physical layout. Provides all dimen-
sional data.
Bill of Material BoM Detailed parts list.
Material Conformity MC Details of compliance for materials.
Third-party component
documentation
Documentation for components produced by a third-party is included where appli-
cable.
1 Introduction
1.3 Associated documentation
8 Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD

2 Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for
your Chromaflow Packing Station. The labels on the instrument and information
regarding recycling are also described.
In this chapter
Section See page
2.1 Safety precautions 10
2.2 Labels 16
2.3 Emergency procedures 18
2 Safety instructions
Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD 9

2.1 Safety precautions
Introduction
The safety precautions in this section are grouped in the following categories:
•General precautions, on page 10
•Flammable liquids and explosive environment, on page 11
•Personal protection, on page 11
•Installing and moving, on page 12
•System operation, on page 13
•Maintenance, on page 14
General precautions
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not operate the product in any other way than described in the
user documentation.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
2 Safety instructions
2.1 Safety precautions
10 Chromaflow Packing Station 50, 100, 200, and 400 Operating Instructions 29046228 AD