cytiva ÄKTA go User manual

ÄKTA go™
Operating Instructions
Original instructions
ÄKTA go
cytiva.com

Table of Contents
1 Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 7
1.4 Abbreviations .................................................................................................................................................. 9
2 Safety instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels and symbols ....................................................................................................................................... 15
2.3 Emergency procedures ............................................................................................................................... 16
3 System description ............................................................................................ 18
3.1 ÄKTA go ............................................................................................................................................................. 19
3.2 Available modules ......................................................................................................................................... 25
3.3 UNICORN .......................................................................................................................................................... 28
4 Installation .......................................................................................................... 32
4.1 Site preparation .............................................................................................................................................. 33
4.1.1 Delivery, storage, and unpacking .......................................................................................................... 34
4.1.2 Space requirements .................................................................................................................................... 36
4.1.3 Site environment .......................................................................................................................................... 39
4.1.4 Power requirements ................................................................................................................................... 41
4.1.5 Computer requirements ........................................................................................................................... 42
4.1.6 Required materials ...................................................................................................................................... 43
4.2 Hardware installation ................................................................................................................................... 44
4.2.1 Connect the system ..................................................................................................................................... 45
4.2.2 Install waste tubing ..................................................................................................................................... 48
4.2.3 Prepare the pump rinsing system ......................................................................................................... 50
4.2.4 Start the instrument .................................................................................................................................... 53
4.3 Software installation .................................................................................................................................... 54
4.3.1 Download and Install UNICORN ............................................................................................................ 55
4.3.2 Download the Instrument Configuration .......................................................................................... 56
4.3.3 Adapt UNICORN to your system ............................................................................................................ 57
4.4 Start UNICORN and connect to system ................................................................................................ 59
5 Prepare the system for a run .............................................................................. 62
5.1 Prepare the flow path ................................................................................................................................... 64
5.2 Prime inlets and purge pump heads ....................................................................................................... 66
5.3 Performance tests ......................................................................................................................................... 72
5.4 Connect a column .......................................................................................................................................... 75
5.5 Pressure alarms .............................................................................................................................................. 78
5.6 Prepare for a run at low temperature ..................................................................................................... 79
6 Run a method ...................................................................................................... 80
6.1 Create a method ............................................................................................................................................ 81
6.2 Prepare sample for loading ........................................................................................................................ 84
6.3 Start a method run ........................................................................................................................................ 86
Table of Contents
ÄKTA go Operating Instructions 29360951 AE 2

6.4 Monitor or interact with the run ............................................................................................................... 87
6.5 Evaluate the run ............................................................................................................................................. 88
6.6 Procedures after the run ............................................................................................................................. 90
7 Maintenance ....................................................................................................... 93
7.1 Clean the instrument externally .............................................................................................................. 94
7.2 Perform system Cleaning-In-Place (CIP) .............................................................................................. 95
7.3 Replace pump rinsing liquid ....................................................................................................................... 99
7.4 Replace the main fuses ................................................................................................................................ 100
8 Troubleshooting ................................................................................................. 102
9 Reference information ....................................................................................... 104
9.1 System specifications .................................................................................................................................. 105
9.2 Chemical resistance specifications ........................................................................................................ 106
9.3 Recycling information .................................................................................................................................. 108
9.4 Regulatory information ............................................................................................................................... 109
9.4.1 Contact information .................................................................................................................................... 110
9.4.2 European Union and European Economic Area .............................................................................. 111
9.4.3 Great Britain ................................................................................................................................................... 112
9.4.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 113
9.4.5 North America ............................................................................................................................................... 115
9.4.6 General regulatory statements .............................................................................................................. 116
9.4.7 South Korea .................................................................................................................................................... 117
9.4.8 China .................................................................................................................................................................. 118
9.4.9 Health and Safety Declaration Form .................................................................................................... 121
Index ........................................................................................................................... 123
Table of Contents
ÄKTA go Operating Instructions 29360951 AE 3

1 Introduction
This chapter contains information about this manual and associated user documenta-
tion, important user information and intended use of ÄKTA go™.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Associated documentation 7
1.4 Abbreviations 9
1 Introduction
ÄKTA go Operating Instructions 29360951 AE 4

1.1 Important user information
Introduction
This section contains important user information about the product and this manual.
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use of the product
The ÄKTA go instrument is intended for the purification of bio-molecules, in particular
proteins, for research purposes. It is intended to be used by trained laboratory staff
members in research laboratories within academia and industry.
The ÄKTA go instrument must not be used in any clinical procedures, or for diagnostic
purposes.
System definition
In this manual, the combination of the ÄKTA go instrument and the UNICORN™ soft-
ware is referred to as the system.
The ÄKTA go instrument without the software is referred to as the instrument.
Prerequisites
In order to operate ÄKTA go in the way it is intended:
•The user must know how to use a computer with the Windows operating system.
•The user must understand the concepts of liquid chromatography.
•The user must read and understand the Safety instructions chapter in the Operating
Instructions.
•The ÄKTA go instrument must be installed in accordance with the site requirements
and instructions in the Operating Instructions.
1 Introduction
1.1Important user information
ÄKTA go Operating Instructions 29360951 AE 5

1.2 About this manual
Introduction
This section contains information about the purpose and scope of this manual, notes
and tips, and typographical conventions.
Purpose of this manual
This manual provides information needed to install, operate and maintain the product
in a safe way.
Scope of this manual
The Operating Instructions is valid for the ÄKTA go system.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Text that the user must either type exactly as shown in the manual, or that the software
displays as a response (not a regular part of the graphic user interface), is shown by a
monospaced typeface (for example, Recipe Information).
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
ÄKTA go Operating Instructions 29360951 AE 6

1.3 Associated documentation
Introduction
This section describes the user documentation delivered with the product, and how to
find related literature that can be downloaded or ordered from Cytiva.
ÄKTA go user documentation
The user documentation listed in the table below is available in printed format or as
PDF file at cytiva.com/aktago under Related Documents.
Translations of the original instructions are given in several languages and are
contained in the CD provided with this manual, or can be found online at cytiva.com/
aktago.
Documentation Main contents
ÄKTA go Unpacking
Instructions (29383543)
Information needed to handle the delivery
package and unpack the ÄKTA go instrument.
ÄKTA go Operating
Instructions
(This instructions)
Information needed to install, operate, and main-
tain the ÄKTA go system in a safe way.
Translations of the original instructions are given
in several languages.
ÄKTA go Cue Cards
(29383545)
Essential information to be kept near the ÄKTA go
system.
ÄKTA go User Manual
(29391392)
Additional detailed information on the system,
component functions, and maintenance. Tips on
how to get the most out of the system when
running it.
ÄKTA go Product
Documentation (29434983)
General specifications and list of materials in the
flow path.
ÄKTA avant, ÄKTA pure
ÄKTA go, and ÄKTA pcc,
Privacy and Security Manual,
29488174
Describes the privacy and security considera-
tions of the use of the system. The manual
describes the expected intended use of the
system, the privacy and security capabilities
included, and how these capabilities are config-
ured.
ÄKTA avant, ÄKTA go, ÄKTA
pcc, and ÄKTA pure Site
Preparation Guide (29117084)
Instructions on how to prepare the installation
site for the ÄKTA go system.
1 Introduction
1.3 Associated documentation
ÄKTA go Operating Instructions 29360951 AE 7

UNICORN user documentation
The UNICORN user documentation is listed in the following table. It is available from
the UNICORN software using the Help drop down menu Contextual Help under the
heading UNICORN contextual help and documentation. It also can be download
from cytiva.com/UNICORN under Related Documents.
Documentation Main contents
UNICORN Quick
Installation Guide 1
Detailed instructions on how to install UNICORN.
UNICORN Administration
and Technical Manual 2•Overview and detailed description of network
setup and complete software installation.
•Administration of UNICORN and the UNICORN
database.
UNICORN Method Manual 2•Overview and detailed descriptions of the
method creation features in UNICORN.
•Workflow descriptions for common operations.
UNICORN System Control
Manual 2•Overview and detailed description of the system
control features in UNICORN.
•Includes general operation, system settings and
instructions on how to perform a run.
UNICORN Evaluation
Manual 2•Overview and detailed descriptions of the
Evaluation Classic 3 module in UNICORN.
•Description of the evaluation algorithms used in
UNICORN.
Getting started with
Evaluation (accessed
through help in the
UNICORN Evaluation
module)
•Video clips showing common workflows in the
Evaluation module.
•Overview of features of the Evaluation module.
UNICORN Help By pressing F1, descriptions are displayed for the
currently active pane or dialog box.
1The UNICORN Quick Installation Guide can be downloaded from cytiva.com/aktago.
2The current UNICORN version is added to the title of the manual.
3Evaluation Classic is an advanced evaluation module that requires an extra license to run.
Additional literature
For practical tips on chromatography, refer to the handbooks available at cytiva.com/
handbooks.
1 Introduction
1.3 Associated documentation
ÄKTA go Operating Instructions 29360951 AE 8

1.4 Abbreviations
Abbreviations
Abbreviation Definition (English) Translation (local language)
CIP Cleaning In Place Cleaning In Place
I/O box Input/Output box Input/Output box
RTU Real-Time Unit Real-Time Unit
UPS Uninterruptible power supply Uninterruptible power supply
1 Introduction
1.4 Abbreviations
ÄKTA go Operating Instructions 29360951 AE 9

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
system. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels and symbols 15
2.3 Emergency procedures 16
Important
WARNING
All users must read and understand the entire contents of
this general safety chapter, and the specific safety precau-
tions information in each subsequent chapter of this manual
to become aware of the hazards involved.
2 Safety instructions
ÄKTA go Operating Instructions 29360951 AE 10