DAKEWE 6250 User manual

Revised Version V4.1-English Version-June 2021
Dakewe(Shenzhen)Medical Equipment Co,. Ltd 1V4.1
Cryostat
User’s Manual
Applicable Model:
6250

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 2 V4.1
Foreword
Dear user,
Thank you for choosing our product!
In order to make you have an overall understanding of the Company’s product to facilitate your use.
We have specially configured this User Manual to the instrument, which includes the instrument’s
structural features, technical characteristics, instructions for use, simple troubleshooting,
maintenance, etc. For your ease of use this instrument.
Before use, be sure to read the User Manual carefully and follow the instructions correctly to the
user manual. In addition, if you have any questions during use, contact technical support, we will
be glad at your service.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 3V4.1
Important Statement
The information, data, and precautions etc. contained in this Manual only represent the scientific
knowledge and advanced technologies that we currently possess through the institutes in this field.
Dakewe (Shenzhen) Medical Equipment Co., Ltd reserves the right to make changes to the
manufacturing process subject to regulatory requirements without prior notice. In this way we can
continuously improve the technology and manufacturing techniques used in our products.
For the error descriptions, charts, technical illustrations, etc. contained in this Manual, organizations
or personnel are encouraged to give us feedback for updating. It is also important to note that we
shall not be liable for any direct or indirect economic loss or damage caused by non-compliance
with statements or other information in this Manual.
This document is protected by copyright law and all copyright in this document belongs to Dakewe
(Shenzhen) Medical Equipment Co., Ltd. Copying the text and illustrations (or any part thereof) of
this document by printing, photocopying, microfilming, webcam or other methods, including any
electronic systems and media, requires prior written permission from Dakewe (Shenzhen) Medical
Equipment Co., Ltd.
For the instrument serial number and year of manufacture, check the nameplate on the back of the
instrument.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 4 V4.1
Table of Contents
1. Information ...............................................................................................................................6
1.1 Symbol and Meaning ........................................................................................................6
1.2 Instrument Use ..................................................................................................................8
1.3 Working principle..............................................................................................................8
1.4 Personnel Qualification.....................................................................................................8
1.5 Instrument Model..............................................................................................................8
1.6 Contraindications ..............................................................................................................8
2. Safety ........................................................................................................................................9
2.1 Safety Precautions.............................................................................................................9
2.2 Warning...........................................................................................................................10
2.2.1 Warning - Signs on the Instrument....................................................................10
2.2.2 Warning – Transport and Installation ................................................................10
2.2.3 Warning - Operation..................................................................................................11
2.2.4 Warning - Cleaning and Maintenance.......................................................................11
2.3 Equipment Placement Requirements ...........................................................................11
2.4 Instrument Handling.....................................................................................................12
2.5 Precautions for Use of the Instrument..........................................................................12
3. Instrument Components and Specifications............................................................................13
3.1 Overview.......................................................................................................................13
3.2 Technical Parameters ...................................................................................................14
4. First Installation....................................................................................................................16
4.1 Installation Requirements..............................................................................................16
4.2 Unpacking Tools ...........................................................................................................16
4.3 Unpacking .....................................................................................................................17
4.4 Accessory Installation ...................................................................................................18
4.4.1 Hand Wheel Installation.........................................................................................18
4.4.2 Hand Wheel Block Installation ..............................................................................19
4.4.3 Unlock the Instrument Cover.................................................................................19
4.4.4 Fixed Card Core.....................................................................................................20
4.4.5 Waste Trough Installation.......................................................................................20
4.4.6 Blade Holder Installation .......................................................................................21
4.4.7 Glass Door Installation...........................................................................................21
4.4.8 Power Supply Cable Connection............................................................................22
4.5 Boot Preparation ...........................................................................................................22
5. Operation.................................................................................................................................23
5.1 Section Function Setting ...................................................................................................23
5.1.1 Cooling Temperature Setting..................................................................................23
5.1.2 Slice and Retouch Setting ......................................................................................24
5.1.3 Cold Stage Temperature Setting / Semiconductor Cooling Function.....................24
5.1.4 Lighting and UV Lamp ..........................................................................................25
5.1.5 Drying Button ........................................................................................................25
5. 2 Button Panel.....................................................................................................................26
5. 3 Function Setting and Introduction....................................................................................26

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 5 V4.1
5.3.1 Defrost Setting .......................................................................................................27
5.3.2 Hibernation Setting ................................................................................................28
5.3.3 Disinfection Setting................................................................................................28
5.3.4 Time reset in the function interface........................................................................29
5. 4 Compressor Overheat Protection Function ......................................................................29
5. 5 System Setting..................................................................................................................30
5.5.1 System Time Setting ..............................................................................................30
5.5.2 Slice Retraction Value Setting................................................................................30
5.6 Blade Holder Module........................................................................................................31
5.6.1 Blade Holder Front and Rear Position Adjustment................................................32
5.6.2 Blade Holder Left and Right Position Adjustment.................................................32
5.6.3 Blade Installation or Replacement .........................................................................32
5.6.4 Anti-roll Plate Adjustment......................................................................................33
5.6.5 Microtome Blade Angle Adjustment......................................................................33
5.7 Sample disk Installation ....................................................................................................34
5.8 Specimen Cryoforming .....................................................................................................35
6. Sectioning Operation...............................................................................................................36
6.1 Starting the Instrument......................................................................................................36
6.2 Preparing Sectioning Tools, Specimen Disc and Auxiliary Preparation Tools..................36
6.3 Retouching Slice ...............................................................................................................36
6.4 Sectioning .........................................................................................................................37
6.5 Sectioning Reference ........................................................................................................37
7. Cleaning and Maintenance......................................................................................................38
7.1 Daily finishing work .........................................................................................................38
7.2 Long-term Suspension of Finishing Work ........................................................................38
7.3 Cleaning Instrument..........................................................................................................38
7.3.1 Before each cleanup, perform the following steps first..........................................38
7.3.2 Cleaning the Instrument Enclosure ........................................................................39
7.3.3 Cleaning the Blade Holder .....................................................................................39
7.4 General Maintenance ........................................................................................................39
7.5 Take the Slicing Core from the Freezer.............................................................................40
7.6 Replacing Component.......................................................................................................41
7.6.1 Replacing the Fuse.................................................................................................41
7.6.2 Replacing the UV Lamp.........................................................................................41
7.7 Use of Waste Tank.............................................................................................................42
8. FAQ and Handling ..................................................................................................................42
9. Section Tissue Temperature Selection Table...........................................................................45
10. Warranty and Service ..............................................................................................................46
10.1 Warranty..........................................................................................................................46
10.2 Technical Service Information ........................................................................................46
10.3 Scrapping and Disposal...................................................................................................46

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 6 V4.1
1. Information
1.1 Symbol and Meaning
Danger:
Indicating an urgent dangerous situation that, if not avoided, will result in
death or serious injury.
Warning:
If you do not avoid this danger, it may result in death or serious injury.
Caution:
Indicating a potentially hazardous situation that, if not avoided, may
result in death or serious injury.
Note:
Indicating information that is not related to risk but is very important.
Follow this User Manual.
Note that see the User Manual for relevant warning information.
IVD medical device
In accordance with appendix four of directive 2002/96/EC of the
European Parliament and Council
Power on
Power off
Biohazard warning (black line on yellow background)
Sign indicator of using flammable solvents and reagents (black line on yellow
background)
This warning symbol indicates the instrument surface that will be hot during
operation.
Avoid direct contact to prevent burns.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 7 V4.1
Environmental protection sign of RoHS Directive. The figure in the symbol
refers to the product’s number of years of “environment-friendly use”. Use this
symbol if the use of a restricted substance exceeds the maximum allowable limit.
Serial number
Manufacturing date
Manufacturer
Expiration
No stacking
Limited height of 2 floors (accessory cases only)
Indicating the correct vertical position of the packing container.
Goods are fragile and must be handled with care.
The package must be stored in a dry environment.
Recycle in accordance with local laws and regulations.
It meets the requirements of EU Directive 98/79/EC for IVD medical devices.
Item indication number in the illustration.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 8 V4.1
1.2 Instrument Use
The instrument consists of 3 major parts:
• Control parts: including related function buttons and display.
• Freezer sections: quickly obtaining low temperature and slicing.
•Refrigeration parts: including refrigeration compressor and semiconductor cooling fin.
The 6250 Semi-automatic Cryostat Microtome is used for rapid freezing of pathological
sections of human and animal body tissue. It can be widely used for preparing thin sections of
samples of varying hardness for use in pathological diagnosis, analysis and research in
hospitals, medical colleges by legal medical experts and propagation institutes.
It is used exclusively by pathology laboratories and is limited to the following tasks:
• Only operate the instrument as described in this Manual.
Any other ways of use are regarded as inappropriate!
1.3 Working principle
After the operator quickly freezes the fresh tissue hard, the frozen hard tissue is placed in a
precision-controlled slicing system, and the tissue is sliced into thin slices of the required
thickness with a blade.
1.4 Personnel Qualification
• Only trained laboratory staff can operate.
• Only operate the instrument as described in this Manual.
1.5 Instrument Model
As described in nameplate.
Product software version
Main control board:V03，Industrial Control Board:V02，Screen board:V03.
1.6 Contraindications
Not found yet.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 9 V4.1
2. Safety
2.1 Safety Precautions
This User Manual contains important information related to operational safety and equipment
maintenance.
As an important part of the product, the User Manual must be read carefully before installation
and use, and always be placed near the instrument.
Electrical safety of instruments conform to IEC 61010-1:2018 Safety requirements for
electrical equipment for measurement, control and laboratory use - Part 1: General
requirements, IEC 61010-2:2018 Safety requirements for electrical equipment for
measurement, control and laboratory - Part 2-101 Special requirements for in vitro diagnostic
(IVD) medical devices".
This instrument meets the requirements for radiated interference and interference immunity
specified in the EMC standards IEC 61326-1:2012 and IEC 61326-2-6:2012
The performance indicators of the instrument are in line with Dakewe's technical requirements
of the cryostat.
In order to maintain this condition and ensure safe operation, the user must comply with all
safety precautions and warnings in this User Manual.
⚫Always observe the safety and precautions described in this Chapter.
⚫Even if you are already familiar with the operation and use of other
Dakewe products, be sure to read these instructions.
⚫Do not remove or modify the protective devices on the instrument and
accessories. Only Dakewe-certified qualified maintenance personnel can
repair the instrument and dispose of the instrument’s internal
components.
⚫The instrument is designed and manufactured with advanced technology
and complies with relevant safety technical standards and specifications.
Improper instrument operation or handling can result in injury to the
user or other personnel, damage to the instrument and other property
damage. The instrument must be used for its intended use and only when
all safety functions are in proper working condition. Faults that are
detrimental to safety must be corrected immediately.
⚫Only original spare parts and allowed original accessories can be used.
⚫Only use original spare parts and permitted original accessories.
Be sure to observe the safety instructions and warnings in this Chapter.
Even if you are already familiar with the operation and use of the product, be sure to read
these instructions.

Dakewe(Shenzhen)Medical Equipment Co,. Ltd 10 V4.1
2.2 Warning
The safety device installed by the manufacturer on this instrument only constitutes the basis of
accident prevention. The primary responsible party for the safe operation is the organization,
followed by the personnel assigned by the organization to operate, maintain or repair the
instrument. In order to ensure the smooth operation of the instrument, be sure to observe the
following instructions and warnings.
2.2.1 Warning - Signs on the Instrument
2.2.2 Warning – Transport and Installation
Do not remove or modify protective devices of the instrument and accessories.
Only authorized maintenance personnel are allowed to repair the instrument
and dispose of the instrument’s internal components.
The warning triangle is displayed on the instrument to ensure that correct
operating instructions defined in this Manual must be observed during
operation and replacement. Failure to follow these instructions may result in
accidents, personal injuries, and damages to the instrument or accessories.
This warning label is used on the surface of the instrument that will be hot
during operation.
Touching these surfaces may cause burns.
The instrument must be kept upright after unpacking.
Do not grasp the handle of the handlewheel or the knob of setting the slice thickness to carry
the instrument.
It is strictly forbidden to disassemble or modify the protective equipment on the instrument
and accessories.