Du Pont SORVALL SUPRASPEED RC-28S User manual

OPERATING
INSTRUCTIONS
SORVALU RC-28S
SUPRASPEED
CENTRIFUGE
Du Pont Company
Sorvall® Instruments
Wilmington, Delaware 19898
U.S.A.
SorvallCentrifuges
PN 59154-1
IssuedAugust1989

Sorvall®Centrifuges
Thismanualisa guideforuseofthe
SORVALL®RC-28S Supraspeed® Centrifuge
Datahereinhasbeenverifiedandvalidatedandisbelieved adequateforthe intended
useofthe centrifuge.Ifthecentrifugeorproceduresareusedfor purposesoverand
abovethe capabilitiesspecified herein, confirmationof their validityand suitability
shouldbe obtained, otherwiseDuPontdoesnot guarantee resultsand assumesno
obligationorliability.This publicationisnota licenseto operate under,nora
recommendationtoinfringe upon,anyprocesspatents.
NOTES, CAUTIONS,andWARNINGS withinthetextofthis manualareusedto
emphasizeimportantand criticalinstructions:
WARNING:AWarning informsthe operatorofa hazardoran unsafe practicethat
couldresultinpersonalinjury,affecttheoperator'shealth,orcontaminate
the environment.
CAUTION:ACautioninformstheoperatorofanunsafepracticethat couldresultin
damageofequipment.
NOTE:
ANotehighlightsessential information.
1988byE.I.duPontdeNemours& Co.
ii

Sorvall®Centrifuges Safety
ImportantSafetyInformation
Certainpotentiallydangerousconditionsareinherenttotheuseofallcentrifuges.To
ensuresafeoperationofthiscentrifuge,anyoneusingitshouldbeawareofallsafe
practicesandtakeallprecautionsdescribedbelowandthroughoutthismanual.
WARNING
UseSorvall®rotorsonly.Useofanother manufacturer's rotorcancause rotor failure
whichcouldresultinpersonal injuryand/orcentrifuge damage.
Whenusingradioactive,toxic,orpathogenicmaterials,beawareofallcharacteristicsof
thematerialsandthehazardsassociatedwiththemintheeventleakageorrotorfailure
occurs during centrifugation,in whichcase neitherthe centrifugenorthe rotorcan
protectyoufrom particles dispersedintheair.To protect yourself, we recommend
additional precautionsbe takento prevent exposureto these materials,e.g.,useof
controlledventilationorisolationareas.
Alwaysbe awareofthe possibilityofcontaminationwhenusing radioactive,toxic,or
pathogenic materials.Takeall necessary precautionsanduse appropriate
decontamination proceduresifexposure occurs.
Neveruseanymaterial capableofproducing flammable orexplosivevapors.
Neverexceedthemaximumrated speedoftheinstalled rotor;todosocancause rotor
failure.
Alwaysreduce(derate)rotor speed whenever:
•the rotor speed/temperature combination exceedsthe solubilityofthe gradient
materialandwould causeittoprecipitate;
• rotor compartmentload exceedsthe maximumallowable compartmentload
specified(checkthiswheneveraverage fluid densityisgreaterthan1.2g/ml).See
Chapter4, Operation.
Failuretoreducerotorspeedundertheseconditionscancauserotor failure.
CAUTION
Do notrunorprecoola rotoratthecriticalspeed,asthiswillhavea detrimentaleffect
on centrifuge componentlife.SeeTable4-1,Rotor Information.
Do notoperatecentrifugewitha rotoroutofbalance.Todosocancausedamagetothe
centrifugedriveassembly.
Do notoperatecentrifuge withoutrotorproperlyinstalled: rotorcovermustbeonand
lockedin place,andthe rotorlockedtothecentrifuge drive.See rotor instruction
manual.
Locatethecentrifugeona level floor toavoidrotorimbalanceduring operation.
Thecentrifugecanbe damagedifconnectedtothe wrongvoltage. Checkthevoltage
before pluggingthe centrifuge intoa power source.DuPontisnot responsiblefor
incorrect installation.
Always maintainthecentrifugeinthe recommended manner.See Chapter7,
Maintenance.
iii/iv

Sorvall®Centrifuges TableofContents
Tableof Contents
Page
SafetyInformationiii
Chapter1.DESCRIPTION
GeneralDescription1-1
CentrifugeSpecifications1-2
CentrifugeFeatures1-3
SystemDescriptions1-5
Chapter2.INSTALLATION
Inspection2-1
Location2-1
AmbientTemperature2-2
Leveling2-2
Electrical2-2
Chapter3.CONTROLS
ControlPanel3-1
PROGRAMKeyFunctions&DisplayScreens3-3
CALCULATEKeyFunctions&DisplayScreens3-9
DIAGNOSTICSKeyFunctions&DisplayScreens3-9
Chapter4.OPERATION
UnderstandingtheCentrifugeControls4-1
TurningtheCentrifugeOn4-1
NormalRun4-1
SlowStart/SlowStop4-3
RCFRun4-5
Key#Code4-6
ZonalRun4-7
HoldRun4-8
fctfdt Run 4-9
ProgrammedRun4-10
ControlPanelLock4-11
Precool/Preheat4-12
ReducingSpeedforRotorCompartmentLoadsin
ExcessofDesignMass4-12

TableofContentsSorvall®Centrifuges
Tableof Contents(continued)
Page
Chapter5.CALCULATOR
CalculatorDisplayScreens5-1
CalculateSpeed/RCFforRotorTime/fo^dt 5-1
CalculateSpeed/RCFata Radius5-2
CalculateKFactor5-2
CalculateRotorConversion/Time5-2
Chapter6.TROUBLESHOOTING& DIAGNOSTICS
DisplayMessages6-1
Diagnostics:
General6-5
Screen1,Faults6-6
Screen2 6-7
Screen3 6-9
HowtoAssignorChangea Key#6-9
HowtoChangeSlowStart/SlowStopTransitionSpeed6-12
Chapter7.MAINTENANCE
MaintenanceSchedule7-1
Cleaning:
RotorChamber7-2
TaperedDriveSpindle7-2
Cabinet7-2
ControlPanel7-2
DoorSeal7-2
Refrigeration SystemCondenser7-4
AirLouvers7-4
Lubrication7-4
ChangingtheVacuumPumpOil& MistEliminatorFilter7-4
EmergencySampleRecovery7-8
TestofRUNDisplayLights& ControlPanelIndicators7-10
PartsOrderingInformation7-11
ServiceDecontaminationPolicy7-11
APPENDIX
VacuumPumpOilSpecifications
Warranty
Glossary
Index
ProgramLogCards
DecontaminationCertificates
VI

SorvairCentrifuges
TableofContents
Listof Illustrations
FigurePage
1-1.SORVALL®RC-28SSupraspeed®Centrifugeviii
2-1. Centrifuge Dimensions2-1
2-2. LevelingAdjustment2-2
3-1. ControlPanel3-2
7-1. DoorSealRemoval7-3
7-2. PartsIdentification, VacuumPumpOilChange7-6
7-3. DoorInterlockOverride7-9
7-4. Vacuum ReleaseSolenoidValveLocation7-9
Listof Tables
TablePage
4-1. RotorInformation4-4
6-1. DIAGNOSTICS,Screen2 Fields6-7
6-2.DIAGNOSTICS,Screen2 Operations6-7
6-3. DIAGNOSTICS,Screen3 Fields6-8
7-1. Maintenance Schedule7-1
vii

Sorvall®Centrifuges
Figure1-1.SORVALL®RC-28SSupraspeed®Centrifuge
viii

Sorvall®CentrifugesDescription
Chapter1
DESCRIPTION
This manual providesyouwiththe informationyouneedto install, operateand
maintainyourSORVALL® RC-28S Supraspeed®Centrifuge.Ifyou encounterany
problemconcerningeitheroperationormaintenancethatisnotcoveredinthemanual,
please telephoneDuPontfor assistance.Inthe United States,calltoll free
[phone]. Outsidethe United States, contactthe distributoror agentfor
SORVALL®Products.
General Description
TheSorvall® RC-28S Supraspeed®Centrifugedefinesa new classof centrifugethat
bringsan unparalleledlevelofperformancetocentrifugation. Createdfromthe latest
designtechnology,itincorporatesusability,dependability,andsafetywithanextremely
versatile speed rangetoseta new standardin value.Whenusedwitha Sorvall®
Supraspeed®rotor,theRC-28Scanreacha topspeedof28000rpmwitha maximum
gforceof100095.At100095x g youcanperform applicationsinthe RC-28Sthat
previouslyrequiredanultracentrifuge.InadditiontotheSupraspeed®rotors designed
forusewiththeRC-28S,theRC-28SacceptsallSorvall®superspeedrotors,increasing
itsusefulnessbymakingitcapableofa widerangeofapplications.
The centrifuge ismicrocomputercontrolledand designedtobeuserfriendly.Itcanbe
operated using basic parametersonlyoryoucan decidetouseits special features.
Becauseofthis versatilityandits user-friendliness,itcan easilybe operatedby
inexperienced persons learning centrifugation methodsorby experienced persons
experimentingwithnewmethods.
Thebrushlessdcdrivewhichofferstheoptimumincentrifuge driveperformanceisone
distinct featureoftheRC-28S. Other featuresofthe centrifuge are: automatic rotor
recognition; programmability;RCFand fco2dt control;slowstartandslowstop control
forsamplereorientation; overtemperature control;a control panellocktopreventrun
parametersfrom beingchanged;anda built-in calculatorthatcanbeusedtoperform
centrifugation calculations, including conversions betweenRCFand speed,timeand
fco2dt orthecalculationofKfactorsandrun timeswhen conversionofpublishedrun
parametersis desired.
The centrifugealsohas extensive diagnostic routinesthatallowtheusertoidentify
systemmalfunctionsandperform maintenanceand repairprocedures.
Formoreonthefeaturesofthe RC-28S,plusmoreonthecentrifugedriveand other
systems,readthetableonthenexttwopagesandthedescriptiveparagraphsthatfollow
thetable.
1-1

Description
Centrifuge Specifications
Son/all®Centrifuges
RotorManagement
SPIN-RIGHT®RotorManagementSystem
automaticallyrecognizesinstalledSorvall®
rotorwhenchamberdoorisclosed.Pre-
ventsoperationofrotorsabovetheirmaxi-
mumspeed.Primaryfunctionofthesystem
is toprovideanaddedmarginofsafety
duringoperation;howeveritiseffectiveonly
whenitisoperatedasintended.Anymis-
useofthesystem,aswithanyothercentri-
fugesystemorfeature,couldresultin
unsafeoperation.
RotorSpeed
Set Speed:
from100rpmto28000rpmin
incrementsof10
Speed Control:
±10rpmfrom0-2000rpm;
±0.5% above2000rpm.
Speed Displayed:
digitalreadoutduringa runofactual
speed
RotorTemperature
Set Temperature:
from-20°Cto+40°C1
Overtemperature:
automaticorsettablefrom0°Cto45°C
Temperature Control:
±10Cfrom2°Cto40°C
Temperature Displayed:
digitalreadoutduringa runofactual
temperaturein 1 ° increments
Time
Set Time:
oneminuteto99hoursand99
minutes;canselectHOLDfor
continuousoperation
Time(continued)
Time Displayed:
digitalreadoutindicatesremaining
timefora timedrunorelapsedtime
forcontinuousrun
Vacuum
Rotaryvanepumpsystem.
Chamber pressure:
<15millibars(1.5kPa)
AmbientTemperature
Range
from15°Cto38°C2
Dimensions:
Width: 70cm(27.5in)
Height:93cm(36.5in)
to topdeck
123cm(48.5in)
to topofcontrolpanel
Depth: 103cm(40.5in)
Clearances:
15 cm(6in)requiredonleftsideof
centrifuge
NetMass
385kg(850lbs)
ElectricalRequirements
Canbesuppliedforuseonthefollowing
singlephasepowersources
200V,60Hz,30A 200V,50Hz,30A
208V,60Hz,30A3220V,50Hz,30A
230V,60Hz,30A3240V,50Hz,30A
Receptacle:
powercordfitsHubbellreceptacle
No. 9330orequivalent
1 Temperature rangewithpowerONbutnoruninprocess(standby)isfrom-10°Cto+30°C.
2Thecentrifugewilloperateatambienttemperaturesupto38°C,butrefrigerationsystemperformancewillbelessthanoptimalabove25°C.
3CSACertificationappliedfor.
1-2

Sorvall®Centrifuges
Description
CentrifugeFeatures
High Performance Drive
Abrushlessdcdirectdrivesystemde-
signedforlongermotorlifeand greater
reliability;provideshightorque;fastac-
celeration;and smooth, precise speed
control.Nobrushesmeanslessmainte-
nance.Controlledbydualmicroproces-
sorswithaccurate speed control during
accelerationanda PositiveActionBrake
systemthatprovidescontrolled braking
fromsetspeedallthewayto0 rpm.
IncludesSorvall®Gyro-Action Self-
CenteringSystemthatpermits"eyebal-
ancing"oftubes. Imbalance detector
protectsagainst damagefrom grossim-
balanceconditions. AcceptsallSorvall®
Superspeedand Supraspeed®rotors.
Automatic Rotor Recognition
SPIN-RIGHT®Rotor Management System
automatically recognizes installed
Sorvall®rotorwhenchamberdooris
closed.Providesoptimal instrument
control. Prohibitsoperationofa rotor
aboveitsmaximum speed. Primary
functionofthesystemistoprovidean
addedmarginofsafety during operation;
however,itiseffectiveonlywhenitis
operatedasintended.Anymisuseofthe
system,aswithanyothercentrifuge
systemorfeature,canresultin unsafe
operation.
Precise Temperature Control
Ahigh-capacity,highlyefficientrefrigera-
tionsystemthat combinesheatingand
cooling.Thecombinedheatingand
coolingprovidestemperature control
from2°Cto40°Cand eliminatesthe
needtodefrostthechamber. Tempera-
turesensorismountedawayfromthe
chamberwalltofurtherimproveaccu-
racyofrotortemperature measurement.
Automaticsampletemperature protec-
tion;maximumtemperaturewillbeset
automaticallyorcanbeentered manu-
ally. Convenientprecoolingofrotors.
Vacuum
AllowsSorvall®Supraspeed®rotorstobe
operatedatspeedsupto28000rpm.
Automaticallyactivatedwhen required.
RCFRun
RCFcanbechoseninplaceofa speedto
controlrun.AllowsRCFtoberepro-
ducedfromruntorunand rotortorotor.
Acceptsvaluesupto100095x g,de-
pendingontherotorused.
SlowStart/ SlowStop
Automatic controlofaccelerationand
decelerationtoandfrom transition
speed;provides optimal reorientation
conditionswhichpreventdisturbanceof
gradientorpellet. Optimaltimestoand
fromtransition speedare automatically
determinedforidentified rotororcanbe
manuallyselected;transition speedcan
evenbechangedifdesired.Flexibilityof
controlmeansyoucanduplicatea slow
start/slowstoprundoneinanyother
centrifuge.
ZonalRun
Allowsoperationwiththechamberdoor
opentodynamicallyloada Sorvall®
TZ-28Zonal Rotor.Loadingspeedselec-
tionfrom1300rpmto2500rpm.Akey
numberisrequiredforzonal operation.
fco2dt Run
Abuilt-inintegratorthat provides exact
run-to-run reproducibilityby controlling
theaccumulating fo)2dt. The accumulat-
ingvalueisdisplayed duringtherun.
Control PanelLock
Locksthecontrolpaneltopreventrun
conditionsfrom beingchanged without
theoperatorknowing.Akeynumberis
requiredtolockand unlockthe control
panel.
1-3

Description
Centrifuge Features
Sorvall®Centrifuges
Programmability
Recordsrunsettingsinmemory.Once
recorded, programscanberecalledto
allowduplicationofrun.Upto20pro-
gramscanberecorded.Akeynumber
is requiredtosavea programtopre-
ventunauthorized changes;however,
programscanberecalledwithouta key
number.
Calculator
Abuilt-incalculatorthat performs
basiccentrifugation calculations:RCF,
Speed,Time, S(o2dU KFactor,andpel-
letingtime.Canbeusedtoconvert
run conditionsfromrotortorotor.
SecurityKeyNumber
Afourdigitsecuritynumberthatis
requiredtoaccessprotecteddataand
somediagnostics.Alsorequiredto
activateZonal,ProgramSave,andthe
Lock.Centrifugeissuppliedwithone
factory-set number,butyoucanpro-
grammultiplenumbersforgreaterse-
curityandmultipleusers.
Advanced Diagnostics
Built-indiagnosticroutines include
severallevelsofelectronictrouble-
shootingaidsthatallowyoutodiag-
noseproblemsiftheyoccur.Alsoused
to performlimitedmaintenanceproce-
duresand centrifuge updates.
RotorChamber
Thestainlesssteelrotorchamberis
epoxycoatedandfreeofwallsensors
makingitdurableandeasytoclean.
Chambersealislocatedinthedoor
ratherthaninthetopofthe chamber;
minimizesaccumulationofdirtonthe
sealwhichimprovestheseal.Thedoor
sealcaneasilyberemovedfor cleaning.
Theslidingchamberdoor retracts
whichmakesthechambereasyto
accessandsolvesdoorclearanceprob-
lems.
Size
TheRC-28Sisnarrowerthanmost
superspeedand ultraspeed centrifuges.
Requireslessfloorspace.
Operator'sGuide
Readilyaccessibleoperatinginstruc-
tionsandaidsthatpulloutfrom under
thecontrolpanel. Includes convenient
ProgramLogrecordsand rotorspecifi-
cations.
Serviceability& Technical
Support
Extensivediagnosticsandeasyto
accesscomponentsmaketheRC-28S
easytoservice. Completeproduct
literatureavailableplusa tollfree
numbertocallfortechnicalassistance.
1-4

Sorvall®CentrifugesDescription
SystemDescriptions
Drive System
The RC-28Sdrive systemuses state-of-the-art brushlessdc motor technologythat
offerstheoptimumincentrifugedrive performance.
A brushlessdc motorusesan electronic commutatorin placeofa mechanical
commutator,whicheliminatesbrushesandthemaintenanceproblemsassociatedwith
them,plusiteliminatesthespeedandcurrentlimitationsofa mechanical commutator.
Thehigh torqueofthedcmotor resultsin faster accelerationand braking,whilethe
highefficiency meanslesspowerconsumptionandlessheat.Lessheatinthe motor
meansthatthedriverunscoolerwhicheliminatestheneedforanyauxiliarymeansof
motorcooling,suchasFreon®orwater.
The motorusedis inherently non-cogging which ensuresthe smoothest acceleration
and deceleration possible.Coggingistypicalofinductionandbrushtypemotorsand
cancausesampledisturbance duringaccelerationand deceleration.
The RC-28Sdrive systemis controlledbydual microprocessorsallowing extremely
accuratespeed controlduringaccelerationand decelerationand eliminating overshoot
of setspeed.Theaccuracyofthespeed controlresultsinsmootherslowstartandslow
stop, pluspositiveactionbrakingfromsetspeedto0 rpm.Themicroprocessorsoperate
independentofeachothertoconstantlymonitorvariouscentrifugefunctionsand,asan
addedsafetyfactor,theymaintainconstantinternalcommunicationwitheachotherto
testand evaluatethe performanceofthe other.Themany teststhatareperformed
routinely throughout operation determineifany preset limitationsareexceeded;any
failuredetectedwillcauseimmediateshutdownbytheprocessordetectingtheproblem.
In addition,thecentrifugewillshutdownifcommunicationfailsbetweentheprocessors
or intheunlikelyeventbothprocessorsfail.
SPIN-RIGHT® RotorManagement System
TheSorvall® RC-28SSPIN-RIGHT®Rotor Management System automatically insures
optimal controlofthe selected speedand temperature parametersand preventsthe
inadvertent settingofa speed beyondthe capabilityofa givenSorvall® rotor.The
Sorvall®rotor installedinthe chamberisrecognized throughan ultrasonic scanning
devicethatis activatedwhenthe chamberdooris closed.The rotor identityisthen
displayedintheSETdisplayofthecontrolpanel.
Theprimaryfunctionoftherotormanagementsystemistoprovideanaddedmarginof
safety duringoperation.Thissystemiseffectiveonlywhenitisoperatedas intended.
Anymisuseofthesystem,aswithanyother centrifuge systemorfeature,can resultin
unsafe operation
Refrigeration System
Therefrigeration systemconsistsof:astainlesssteelevaporatorthatactsastherotor
chamber,a 2-hp hermetically sealed compressor,anair-cooled condenser,andthe
suction-capillarytubeassembly.Ahotgasbypasscircuitallowsforquiet, non-cycling
1-5

DescriptionSorvall®Centrifuges
compressoroperationaswellasimprovedtemperaturecontrolandfrost-free operation.
Therefrigeration systemisfactory chargedwithFreon®502.
Thedesiredrun temperatureisenteredonthecontrolpanelandismaintainedbythe
systemelectronicswhichcyclesthehotgascircuitasrequired.Ifdesired,a maximum
temperaturecanbeenteredonthecontrolpanel;ifa valueisnotenteredmanually,the
maximumtemperaturewilldefaultto+5°Cabovesettemperature.Anyfailure causing
thetemperaturetoexceedthemaximumsettingwillresultintheterminationoftherun
anda corresponding messagewillbe displayedonthe control panelto alertthe
operator.
Vacuum System
Thevacuumsystemincludesa directdrivevacuumpump,anair-inletsolenoid(bleed)
valve,misteliminator,theevaporatorchamber,andinterconnectingtubesand fittings.
Reducedrotorchamberpressureissensedbya Piraniconvectiongaugelocatedatthe
pump inletmanifoldand monitoredbythe machine control microprocessor.When
activated,thissystemprovidesa vacuumlevelbetween1and15millibars.
Thevacuum systemisautomatically activatedonlywhenthesetspeedishigherthan
thetopairspeedofoneoftheSorvall®Supraspeed®rotorsdesignedspecificallyforuse
withtheRC-28S.
In the eventofa vacuumlossorinsufficient vacuum,a messagewillappearinthe
control panelSETdisplaytonotifythe operator.Inan emergency,suchasa power
failure,whensamplerecoveryisdesirable,thechamberdoorcanbeopened manually
(seeChapter7 forprocedure).
1-6

Sorvall®Centrifuges Installation
Chapter2
INSTALLATION
ThischaptercontainsinstructionstoprepareyourSorvall®RC-28Sfor operation.
Inspection
Whenyou receiveyour centrifuge,carefully inspectitforany signsof shipping
damage.Ifyoufind damage, please reportit immediatelytothe transportation
companyandfilea damageclaim,thennotifyDuPont.
Checkthe partsreceivedwiththe centrifuge againstthe shipping list;ifany parts
aremissing,contactDuPont(seebackcover).
Location
Locatethe centrifugeona level floor. Topermitfreeair circulation,the locationmust
allowspaceforthesizeofthecentrifuge(figure2-1),plusanadditional15cm(6inch)
spaceontheleftsideofthe centrifuge.Freeair circulationisveryimportantforthe
centrifugetofunction properly.
LEFTSIDEOF
CENTRIFUGE
POWER
CORD
200cm-
(78.5in)
123cm
(48.5in)
Figure2-1.Centrifuge Dimensions
2-1

Installation
Son/all®Centrifuges
AmbientTemperature
An ambient temperature rangeof15°Cto38°Cmustbe maintained.Ifthe inletair
temperatureisabove25°C,thecentrifugewillnot maintainlow temperaturesat
highspeeds.
Leveling
WARNING
Thefront stabilizersmustbeusedas explainedbelowtoliftthe castersoffthe
floor;thiswillhelp minimizecentrifuge movementinthe eventofa rotor
failure.
Whenthe centrifuge is situatedatits operating location,lowerand securethefront
lockingstabilizers.Lowerthetwolockingstabilizersbyhanduntiltheyarefinger tight
againstthefloor.Thenusethe9/16inch(14mm)wrenchsuppliedwiththecentrifuge
to rotateeach stabilizeran additional2 1/2 turns.Thiswillraisethefront casters
about3 mm(1/8inch)offthefloorandlevelthecentrifuge (basedonlevelfloor).
LOCKING STABILIZERS
(9/16in[14mm]HEX)
APPROX.3mm(1/8in)
OFFTH EFLOOR
Figure2-2.LevelingAdjustment
Electrical
Your centrifugewas orderedfora specific single phasepower source(preferably
regulated).The nameplateontheback panelofthe centrifuge tells whichoneof
thefollowingclassificationsyou ordered:
200V,60Hz,30A
208V,60Hz,30A*
230V,60Hz,30A*
200V,50Hz,30A
220V,50Hz,30A
240V,50Hz,30A
If thelinevoltagevaries±10%ofits nominal value,youmay noticevariationsinthe
performanceofthecentrifuge.Ifthelinevoltageexceedsthis tolerance,itmay
damagethecentrifuge.
*CSACertificationappliedfor.
2-2

Sorvall®C e n t r i f u g e sinstallation
CAUTION
The centrifuge canbedamagedifconnectedtothewrongvoltage. Check
thevoltagebeforepluggingthecentrifugeintoanypowersource. DuPont
is notresponsibleforimproper installation.
The centrifugeis equippedwitha 3-wirepowercordwitha 3-prongcap, including
ground connection,tofit HubbellreceptacleNo.9330or equivalent.
To connectthecentrifugetoa voltageotherthanwhatisspecifiedonthenameplate,the
centrifugewillhavetoberewiredandthepowercordmayhavetobereplaced.Inthis
case, contactDuPonttohavea ServiceRepresentativedotherewiring.
The ON/OFFmainpower switchisa 30A circuit breaker, thereforea separateline
disconnect switchisnotneededunlessrequiredbylocalcodes.
2-3/2-4

Sorvall®Centrifuges Controls
Chapter3
CONTROLS
Thischapterprovides:
•adetaileddescriptionoftheRC-28Scontrols;
•abriefdescriptionofthebuilt-incalculator;and
•abriefdescriptionofthediagnosticroutines.
For step-by-step operating instructionssee Chapter4,andfor further information
on thecalculatorandthediagnostics,seeChapters5 and6.
NOTE
A convenient Operator's Guide containingsimplified instructionsis located
underthecentrifuge controlconsole.
ControlPanel
The RC-28Siseasytouse.All commandsare entered from the convenient keypad
on the control panelandthesetandrun parametersare visibleinthe displays.All
selected parameterscanbelockedto prevent changestorun settings. Figure3-1
on thenextpage showsthe RC-28S control panelandgivesa brief descriptionof
thekeysand displays.Acompletedescriptionfollowstheillustration.
Set Display
TheSETdisplayisthelower displaythat changesby pressingthePROGRAM,
CALCULATEorDIAGNOSTICSkeytoshow various screensof parametersor
operations.A cursorintheSETdisplay indicatesthefield wherethenext input
characterwill appear.Lightsbelowthe display indicatethe positionofthe cursor.
RunDisplay
TheRUN display includesthe three upper displaysthatshow actual speed,
temperature,andtimeorJco2dt duringa run.
3-1

CO
2
1
B
I
SCREEN INDICATOR
Indicates which screen
hasbeenselectedand
appearsinSETdisplay.
CURSOR INDICATOR
Indicatescursorlocationin
SETdisplay.
PROGRAM
Primarycontrolkey.Se-
lectsonescreenof basic
andthree screensofop-
tionalrun parameters.
Display changeseach
timekeyispressedanda
corresponding indicator
lightabovet h ekeycomes
on to indicate which
screenisinthedisplay.
CALCULATE
Selectscalculatormodet o
performbasic centrifuga-
tion calculations.Four
screensof calculations
available.Display chan-
geseachtimekeyis
pressedanda corre-
spondingindicatorlight
aboveth ekeycomeso nt o
indicatewhichscreenisin
display.
SETDISPLAY
Displays parametersto
be selected, calculator
modes,and diagnostic
conditions. Changedis-
playby pressingoneof
thethreecontrolkeysbe-
lowthedisplay.
RUN DISPLAY
Speed
Displays current rotor
speed(rpm)duringa run.
Temperature
Displays current rotor
temperature(°C)duringa
run.
Time
TIMEDRun: displaysre-
mainingruntime.
HOLDRun: displaysac-
cumulatingruntime.
displaysaccu-
mulatingintegralvalue.
O
O
a
Ja/WflNDICATOR
IndicatorlightisONwhen
lco2dt runisselected.
START
Startsthecentrifugerun.
STOP
Stopsthe centrifugerun.
NUMERICKEYPAD
Usedtoinputnumbersto
selectparameters.
HOLD
Selectsa continuousrun.
DIAGNOSTICS
Selects diagnosticmode
to help isolatemalfunc-
tions, provide statusof
various systems,andto
performmaintenancepro-
cedures.
ENTER
Movesthecursorrightor
leftthroughSET display.
Also causesan input
valuetobeaccepted.
ON/OFF+/-
The+/ -changessettem -
peraturevaluesfromplus
to minusorfromminusto
plus. TheOn/Off selects
brakemodeand other
options.
CLEAR
Cancelsaninputora pre-
viously entered value.
CO
o
5
(D
CO

Sorvall®Centrifuges
Controls
DescriptionofDisplay& KeyFunctions
The numerickeypad is used to input numbersfrom 0 to9 to
v jv jV j selectparametervalues.
TheENTERkeys cause an input value tobe
accepted,plusthey cause the cursor to move to
theright ortothe leftthrough theSET display.
On/Off is used to select options.When pressed, the
word OnorOff will appear inthe appropriatefield
of theSET display. The+/-is usedonly to change
the temperature valuefrompositive to negative or
negative to positive.
Cancels an input ora previously entered value.When thekeyis pressed,
the entry issetto zero (0)anda new value canbe input.When pressed
again, the previouslyenteredvalue is restored.
Starts the centrifuge run.
Stops the centrifuge run.
Selects a continuous run.The wordHoldwill appear inthe HnMinfield of
the SET display andall zeroswill beintheRUN display. As soon as START
is pressed, the timerwill start andthe Time field oftheRUN displaywill
showaccumulating run time.
PROGRAM
Program Key Functions & Disp lay Screens
Selectsfour screens of standard and optionalcentrifugation conditions. The
screens are labeled 1,2,3,and4 and have corresponding lightsabove thekey that
come onto indicate which screen you have accessed.Eachtime thekeyis pressed,
the SET displaywillchange tothe nextscreen.
ThefourPROGRAM key screens are illustratedbelow showing the available
parameters and operations. A description ofthe fields in eachscreenfollows.
PROGRAMDISPLAYSCREENS
1
2
3
4
RotorSpeedHr:MinTenp/MaxBrake
RCFSlow-StartSlow-StopZonal/Speed
w2dtTine-outTotalLock
PROGRflM>Recal1#Save#
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ControlsSorvall®Centrifuges
PROGRAM
ProgramKeyFunctions& DisplayScreens
Screen 1
Rotor Speed HrsMin Temp^Max Brake
F-28^13 23000 0:90 + 4 + 9 On
Whenthe"1"indicatorlightabovethePROGRAMkey is on,theSETdisplay screen
will include the basic run parameters: rotoridentification, speed,time,
temperature, and brake. All parameters, except rotor identification, canbe
changedduring a run.
Followingis a descriptionofeachfieldinthisscreen.
RotorSpeedHrsMinTeMp/MaxBrake
TheSPIN-RIGHT®Rotor Management System automatically recognizesthe installed
Sorvall® rotor to provide temperature compensation,top speedlimit, speed control,
andRCFcalculation.Whenthe chamberdoor is c l o s e d ,the rotornamewill appear
in the display. Wheneverthe chamberdoor is open,thewordNONEwillappear i n
the display unlesstheLock is on, in whichcasethe rotornamewillstay in the
display.
Therotoridentification cannotbechangedafterSTARTh a sbeenpressed.
IliiiiiilSpeed
Centrifugation speed is selected by entering a valuefrom100 to 28000rpm in
increments of 10rpm. Ifa speed value greaterthanthe maximum allowable speed
of the installed rotor is entered,alldigits in t h e Speedfieldwill flash indicatingan
erroneous entry. In t h i s case, clearthe entryandmake a valid entry.
NOTE
If anRCFvalue is selected to controltherun,the speed required to achieve
thechosenRCFvaluewillautomaticallybecalculatedanddisplayedinthisfield.
The speed settingcan be changedafter a runhasbeen startedby returning to t h e
Speedfieldand entering a n e wvalue.
RotorSpeedHrsMin TerwMaxBrake
Centrifugationtimecan bes e t f r o mone minute to99 hoursand 99 minutes.
Valuesare displayedexactly as entered; for example, if you input90, it will b e
displayed0:90 whichrepresentsonehourand30minutesrun time.Thesamerun
time of onehourand 30 minutescan be selected by entering130;thiswill b e
displayedas1:30.
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