Du Pont SORVALL SUPRASPEED RC-28S User manual

OPERATING
INSTRUCTIONS
SORVALU RC-28S
SUPRASPEED
CENTRIFUGE
Du Pont Company
Sorvall® Instruments
Wilmington, Delaware 19898
U.S.A.
SorvallCentrifuges
PN 59154-1
IssuedAugust1989

Sorvall®Centrifuges
Thismanualisa guideforuseofthe
SORVALL®RC-28S Supraspeed® Centrifuge
Datahereinhasbeenverifiedandvalidatedandisbelieved adequateforthe intended
useofthe centrifuge.Ifthecentrifugeorproceduresareusedfor purposesoverand
abovethe capabilitiesspecified herein, confirmationof their validityand suitability
shouldbe obtained, otherwiseDuPontdoesnot guarantee resultsand assumesno
obligationorliability.This publicationisnota licenseto operate under,nora
recommendationtoinfringe upon,anyprocesspatents.
NOTES, CAUTIONS,andWARNINGS withinthetextofthis manualareusedto
emphasizeimportantand criticalinstructions:
WARNING:AWarning informsthe operatorofa hazardoran unsafe practicethat
couldresultinpersonalinjury,affecttheoperator'shealth,orcontaminate
the environment.
CAUTION:ACautioninformstheoperatorofanunsafepracticethat couldresultin
damageofequipment.
NOTE:
ANotehighlightsessential information.
1988byE.I.duPontdeNemours& Co.
ii

Sorvall®Centrifuges Safety
ImportantSafetyInformation
Certainpotentiallydangerousconditionsareinherenttotheuseofallcentrifuges.To
ensuresafeoperationofthiscentrifuge,anyoneusingitshouldbeawareofallsafe
practicesandtakeallprecautionsdescribedbelowandthroughoutthismanual.
WARNING
UseSorvall®rotorsonly.Useofanother manufacturer's rotorcancause rotor failure
whichcouldresultinpersonal injuryand/orcentrifuge damage.
Whenusingradioactive,toxic,orpathogenicmaterials,beawareofallcharacteristicsof
thematerialsandthehazardsassociatedwiththemintheeventleakageorrotorfailure
occurs during centrifugation,in whichcase neitherthe centrifugenorthe rotorcan
protectyoufrom particles dispersedintheair.To protect yourself, we recommend
additional precautionsbe takento prevent exposureto these materials,e.g.,useof
controlledventilationorisolationareas.
Alwaysbe awareofthe possibilityofcontaminationwhenusing radioactive,toxic,or
pathogenic materials.Takeall necessary precautionsanduse appropriate
decontamination proceduresifexposure occurs.
Neveruseanymaterial capableofproducing flammable orexplosivevapors.
Neverexceedthemaximumrated speedoftheinstalled rotor;todosocancause rotor
failure.
Alwaysreduce(derate)rotor speed whenever:
•the rotor speed/temperature combination exceedsthe solubilityofthe gradient
materialandwould causeittoprecipitate;
• rotor compartmentload exceedsthe maximumallowable compartmentload
specified(checkthiswheneveraverage fluid densityisgreaterthan1.2g/ml).See
Chapter4, Operation.
Failuretoreducerotorspeedundertheseconditionscancauserotor failure.
CAUTION
Do notrunorprecoola rotoratthecriticalspeed,asthiswillhavea detrimentaleffect
on centrifuge componentlife.SeeTable4-1,Rotor Information.
Do notoperatecentrifugewitha rotoroutofbalance.Todosocancausedamagetothe
centrifugedriveassembly.
Do notoperatecentrifuge withoutrotorproperlyinstalled: rotorcovermustbeonand
lockedin place,andthe rotorlockedtothecentrifuge drive.See rotor instruction
manual.
Locatethecentrifugeona level floor toavoidrotorimbalanceduring operation.
Thecentrifugecanbe damagedifconnectedtothe wrongvoltage. Checkthevoltage
before pluggingthe centrifuge intoa power source.DuPontisnot responsiblefor
incorrect installation.
Always maintainthecentrifugeinthe recommended manner.See Chapter7,
Maintenance.
iii/iv

Sorvall®Centrifuges TableofContents
Tableof Contents
Page
SafetyInformationiii
Chapter1.DESCRIPTION
GeneralDescription1-1
CentrifugeSpecifications1-2
CentrifugeFeatures1-3
SystemDescriptions1-5
Chapter2.INSTALLATION
Inspection2-1
Location2-1
AmbientTemperature2-2
Leveling2-2
Electrical2-2
Chapter3.CONTROLS
ControlPanel3-1
PROGRAMKeyFunctions&DisplayScreens3-3
CALCULATEKeyFunctions&DisplayScreens3-9
DIAGNOSTICSKeyFunctions&DisplayScreens3-9
Chapter4.OPERATION
UnderstandingtheCentrifugeControls4-1
TurningtheCentrifugeOn4-1
NormalRun4-1
SlowStart/SlowStop4-3
RCFRun4-5
Key#Code4-6
ZonalRun4-7
HoldRun4-8
fctfdt Run 4-9
ProgrammedRun4-10
ControlPanelLock4-11
Precool/Preheat4-12
ReducingSpeedforRotorCompartmentLoadsin
ExcessofDesignMass4-12

TableofContentsSorvall®Centrifuges
Tableof Contents(continued)
Page
Chapter5.CALCULATOR
CalculatorDisplayScreens5-1
CalculateSpeed/RCFforRotorTime/fo^dt 5-1
CalculateSpeed/RCFata Radius5-2
CalculateKFactor5-2
CalculateRotorConversion/Time5-2
Chapter6.TROUBLESHOOTING& DIAGNOSTICS
DisplayMessages6-1
Diagnostics:
General6-5
Screen1,Faults6-6
Screen2 6-7
Screen3 6-9
HowtoAssignorChangea Key#6-9
HowtoChangeSlowStart/SlowStopTransitionSpeed6-12
Chapter7.MAINTENANCE
MaintenanceSchedule7-1
Cleaning:
RotorChamber7-2
TaperedDriveSpindle7-2
Cabinet7-2
ControlPanel7-2
DoorSeal7-2
Refrigeration SystemCondenser7-4
AirLouvers7-4
Lubrication7-4
ChangingtheVacuumPumpOil& MistEliminatorFilter7-4
EmergencySampleRecovery7-8
TestofRUNDisplayLights& ControlPanelIndicators7-10
PartsOrderingInformation7-11
ServiceDecontaminationPolicy7-11
APPENDIX
VacuumPumpOilSpecifications
Warranty
Glossary
Index
ProgramLogCards
DecontaminationCertificates
VI

SorvairCentrifuges
TableofContents
Listof Illustrations
FigurePage
1-1.SORVALL®RC-28SSupraspeed®Centrifugeviii
2-1. Centrifuge Dimensions2-1
2-2. LevelingAdjustment2-2
3-1. ControlPanel3-2
7-1. DoorSealRemoval7-3
7-2. PartsIdentification, VacuumPumpOilChange7-6
7-3. DoorInterlockOverride7-9
7-4. Vacuum ReleaseSolenoidValveLocation7-9
Listof Tables
TablePage
4-1. RotorInformation4-4
6-1. DIAGNOSTICS,Screen2 Fields6-7
6-2.DIAGNOSTICS,Screen2 Operations6-7
6-3. DIAGNOSTICS,Screen3 Fields6-8
7-1. Maintenance Schedule7-1
vii

Sorvall®Centrifuges
Figure1-1.SORVALL®RC-28SSupraspeed®Centrifuge
viii

Sorvall®CentrifugesDescription
Chapter1
DESCRIPTION
This manual providesyouwiththe informationyouneedto install, operateand
maintainyourSORVALL® RC-28S Supraspeed®Centrifuge.Ifyou encounterany
problemconcerningeitheroperationormaintenancethatisnotcoveredinthemanual,
please telephoneDuPontfor assistance.Inthe United States,calltoll free
[phone]. Outsidethe United States, contactthe distributoror agentfor
SORVALL®Products.
General Description
TheSorvall® RC-28S Supraspeed®Centrifugedefinesa new classof centrifugethat
bringsan unparalleledlevelofperformancetocentrifugation. Createdfromthe latest
designtechnology,itincorporatesusability,dependability,andsafetywithanextremely
versatile speed rangetoseta new standardin value.Whenusedwitha Sorvall®
Supraspeed®rotor,theRC-28Scanreacha topspeedof28000rpmwitha maximum
gforceof100095.At100095x g youcanperform applicationsinthe RC-28Sthat
previouslyrequiredanultracentrifuge.InadditiontotheSupraspeed®rotors designed
forusewiththeRC-28S,theRC-28SacceptsallSorvall®superspeedrotors,increasing
itsusefulnessbymakingitcapableofa widerangeofapplications.
The centrifuge ismicrocomputercontrolledand designedtobeuserfriendly.Itcanbe
operated using basic parametersonlyoryoucan decidetouseits special features.
Becauseofthis versatilityandits user-friendliness,itcan easilybe operatedby
inexperienced persons learning centrifugation methodsorby experienced persons
experimentingwithnewmethods.
Thebrushlessdcdrivewhichofferstheoptimumincentrifuge driveperformanceisone
distinct featureoftheRC-28S. Other featuresofthe centrifuge are: automatic rotor
recognition; programmability;RCFand fco2dt control;slowstartandslowstop control
forsamplereorientation; overtemperature control;a control panellocktopreventrun
parametersfrom beingchanged;anda built-in calculatorthatcanbeusedtoperform
centrifugation calculations, including conversions betweenRCFand speed,timeand
fco2dt orthecalculationofKfactorsandrun timeswhen conversionofpublishedrun
parametersis desired.
The centrifugealsohas extensive diagnostic routinesthatallowtheusertoidentify
systemmalfunctionsandperform maintenanceand repairprocedures.
Formoreonthefeaturesofthe RC-28S,plusmoreonthecentrifugedriveand other
systems,readthetableonthenexttwopagesandthedescriptiveparagraphsthatfollow
thetable.
1-1

Description
Centrifuge Specifications
Son/all®Centrifuges
RotorManagement
SPIN-RIGHT®RotorManagementSystem
automaticallyrecognizesinstalledSorvall®
rotorwhenchamberdoorisclosed.Pre-
ventsoperationofrotorsabovetheirmaxi-
mumspeed.Primaryfunctionofthesystem
is toprovideanaddedmarginofsafety
duringoperation;howeveritiseffectiveonly
whenitisoperatedasintended.Anymis-
useofthesystem,aswithanyothercentri-
fugesystemorfeature,couldresultin
unsafeoperation.
RotorSpeed
Set Speed:
from100rpmto28000rpmin
incrementsof10
Speed Control:
±10rpmfrom0-2000rpm;
±0.5% above2000rpm.
Speed Displayed:
digitalreadoutduringa runofactual
speed
RotorTemperature
Set Temperature:
from-20°Cto+40°C1
Overtemperature:
automaticorsettablefrom0°Cto45°C
Temperature Control:
±10Cfrom2°Cto40°C
Temperature Displayed:
digitalreadoutduringa runofactual
temperaturein 1 ° increments
Time
Set Time:
oneminuteto99hoursand99
minutes;canselectHOLDfor
continuousoperation
Time(continued)
Time Displayed:
digitalreadoutindicatesremaining
timefora timedrunorelapsedtime
forcontinuousrun
Vacuum
Rotaryvanepumpsystem.
Chamber pressure:
<15millibars(1.5kPa)
AmbientTemperature
Range
from15°Cto38°C2
Dimensions:
Width: 70cm(27.5in)
Height:93cm(36.5in)
to topdeck
123cm(48.5in)
to topofcontrolpanel
Depth: 103cm(40.5in)
Clearances:
15 cm(6in)requiredonleftsideof
centrifuge
NetMass
385kg(850lbs)
ElectricalRequirements
Canbesuppliedforuseonthefollowing
singlephasepowersources
200V,60Hz,30A 200V,50Hz,30A
208V,60Hz,30A3220V,50Hz,30A
230V,60Hz,30A3240V,50Hz,30A
Receptacle:
powercordfitsHubbellreceptacle
No. 9330orequivalent
1 Temperature rangewithpowerONbutnoruninprocess(standby)isfrom-10°Cto+30°C.
2Thecentrifugewilloperateatambienttemperaturesupto38°C,butrefrigerationsystemperformancewillbelessthanoptimalabove25°C.
3CSACertificationappliedfor.
1-2

Sorvall®Centrifuges
Description
CentrifugeFeatures
High Performance Drive
Abrushlessdcdirectdrivesystemde-
signedforlongermotorlifeand greater
reliability;provideshightorque;fastac-
celeration;and smooth, precise speed
control.Nobrushesmeanslessmainte-
nance.Controlledbydualmicroproces-
sorswithaccurate speed control during
accelerationanda PositiveActionBrake
systemthatprovidescontrolled braking
fromsetspeedallthewayto0 rpm.
IncludesSorvall®Gyro-Action Self-
CenteringSystemthatpermits"eyebal-
ancing"oftubes. Imbalance detector
protectsagainst damagefrom grossim-
balanceconditions. AcceptsallSorvall®
Superspeedand Supraspeed®rotors.
Automatic Rotor Recognition
SPIN-RIGHT®Rotor Management System
automatically recognizes installed
Sorvall®rotorwhenchamberdooris
closed.Providesoptimal instrument
control. Prohibitsoperationofa rotor
aboveitsmaximum speed. Primary
functionofthesystemistoprovidean
addedmarginofsafety during operation;
however,itiseffectiveonlywhenitis
operatedasintended.Anymisuseofthe
system,aswithanyothercentrifuge
systemorfeature,canresultin unsafe
operation.
Precise Temperature Control
Ahigh-capacity,highlyefficientrefrigera-
tionsystemthat combinesheatingand
cooling.Thecombinedheatingand
coolingprovidestemperature control
from2°Cto40°Cand eliminatesthe
needtodefrostthechamber. Tempera-
turesensorismountedawayfromthe
chamberwalltofurtherimproveaccu-
racyofrotortemperature measurement.
Automaticsampletemperature protec-
tion;maximumtemperaturewillbeset
automaticallyorcanbeentered manu-
ally. Convenientprecoolingofrotors.
Vacuum
AllowsSorvall®Supraspeed®rotorstobe
operatedatspeedsupto28000rpm.
Automaticallyactivatedwhen required.
RCFRun
RCFcanbechoseninplaceofa speedto
controlrun.AllowsRCFtoberepro-
ducedfromruntorunand rotortorotor.
Acceptsvaluesupto100095x g,de-
pendingontherotorused.
SlowStart/ SlowStop
Automatic controlofaccelerationand
decelerationtoandfrom transition
speed;provides optimal reorientation
conditionswhichpreventdisturbanceof
gradientorpellet. Optimaltimestoand
fromtransition speedare automatically
determinedforidentified rotororcanbe
manuallyselected;transition speedcan
evenbechangedifdesired.Flexibilityof
controlmeansyoucanduplicatea slow
start/slowstoprundoneinanyother
centrifuge.
ZonalRun
Allowsoperationwiththechamberdoor
opentodynamicallyloada Sorvall®
TZ-28Zonal Rotor.Loadingspeedselec-
tionfrom1300rpmto2500rpm.Akey
numberisrequiredforzonal operation.
fco2dt Run
Abuilt-inintegratorthat provides exact
run-to-run reproducibilityby controlling
theaccumulating fo)2dt. The accumulat-
ingvalueisdisplayed duringtherun.
Control PanelLock
Locksthecontrolpaneltopreventrun
conditionsfrom beingchanged without
theoperatorknowing.Akeynumberis
requiredtolockand unlockthe control
panel.
1-3