DUTSCHER FlexA-200 User manual

I
Operation Manual
Version 1.0
FlexA-200
Microplate Reader
DOMINIQUE DUTSCHER SAS

II
Foreword
Thank you for purchasing our Microplate Reader. This user manual describes how the
instrument works and the operation guide, please read carefully before operation and
keep for future reference.
Opening check
Please check the instruments as well as all accessories with packing list when you first
open it. If you find any wrong or missing, please contact distributor or manufacturer.
File No.: AS148SM
Version No.: Dec. 2019
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III
Safety Warning and Guidelines
1. Important information for safe use
Any improper operation without reading manual is forbidden, otherwise there
will be risks in cause accidental injury or electrical shock.
Do read manual carefully and operate safely according to this guidelines.
This instrument intended to use in Scientific Research Only!
2. Safety Tips
The operation, maintenance and repair of the Instrument should comply with the basic
guidelines and the remarked warning below. If you don’t comply with them, it will have
effect on the scheduled using life of the Instrument and the protection provided.
Indoor use only.
Warning: Biological contamination!! All samples for test, quality control,
calibration are regarded as infectious, and any part contact with samples
will also need to be treated as infectious. Please wear gloves when operate
this device.
Before using the device, read the Manual carefully. These units are
designed for use in laboratory environments. The device must be used by
skilled personnel with the appropriate training.
Warning: Avoid injury. Keep your body or any part of body away 15cm (or
more) from the instrument when running.
Users should have a clear main idea on how to use this instrument before
operate, do read this user manual carefully.
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IV
The operator should not open or repair the Instrument by himself, which
will result in losing the qualification of repair guarantee or occur accident.
If there is some wrong with the Instrument, please contact manufacturer
for repair.
Before power on, guarantee the voltage used should be accordant to the
voltage needed, and the rated load of electrical outlet should not lower
than the demand.
If the electric line is damaged, you should replace it with the same type. You
should assure there’s nothing on the electric line and you should not put
the electric line in the ambulatory place.
Hold the socket when you pull out the plug, and don’t pull the electric line
only.
The Instrument should be put in the place of low temperature, less dust, no
water and no sun or strong lamp. What’s more, the place should be good
ventilation, no corrosively gas or strong disturbing magnetic field, far away
from central heating, camp stove and other hot resource.
Power off when you finish your work. Pull off the connector plug when
there’s long time no use of the Instrument and cover it with a cloth or
plastic paper to prevent from dust.
Pull the connector plug from the socket at once in the following cases, and
contact the vendor:
There is some liquid flowing into the Instrument;
Drenched or fire burned.
Abnormal operation: such as abnormal sound or smell.
Instrument dropping or outer shell damaged.
Malfunction
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Contents
Foreword.................................................................................................................................................... II
Opening check............................................................................................................................................II
Safety Warning and Guidelines................................................................................................................ III
1. Important information for safe use.............................................................................................. III
2. Safety Tips..................................................................................................................................... III
Chapter 1 Brief Introduction....................................................................................................................1
Chapter 2 Features.................................................................................................................................. 2
Chapter 3 Instrument Structure.............................................................................................................. 3
Chapter 4 Installation.............................................................................................................................. 4
1. Opening check................................................................................................................................ 4
2. Installation...................................................................................................................................... 4
3. Installation steps............................................................................................................................. 4
Chapter 5 Operation Guide..................................................................................................................... 6
1. Instrument self-check..................................................................................................................... 6
2. System settings............................................................................................................................... 7
3. Protocol Management.................................................................................................................... 8
4. Read a Microplate.........................................................................................................................10
5. Result processing.......................................................................................................................... 16
6. Report exporting...........................................................................................................................23
7. Power off.......................................................................................................................................23
Chapter 6 Maintenance, storage, transportation................................................................................. 24
1. Maintenance................................................................................................................................. 24
2. Storage and transportation.......................................................................................................... 24
Chapter 7 Trouble shooting................................................................................................................... 25
DOMINIQUE DUTSCHER SAS

FlexA-200 Microplate Reader Operation Manual Chapter 1 Brief Introduction
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Chapter 1 Brief Introduction
This automatic microplate reader FlexA-200 is professional instrument for ELISA ,
measuring concentration, absorbance, positive or negative of the antibody and antigen
in the sample by testing the color of the Enzyme - Linked Immunosorbent Assay (ELISA).
This reader is widely used in clinical test, biology agriculture, food and environment
research, especially benefit from ELISA kits increasingly wide utilization.
Highlights:
1) With 10-inch touch screen.
2) Operating system allows acquisition, editing and saving of data.
3) It can be used alone, and also connect with PC by ReaderIt-II software for
plenty of data analysis.
4) 96-well visual layout allows easy setting of blank, sample, positive/negative,
quality control and multi-value comparison.
5) With dual optical system, as well as reference optical channel which guarantee
stable detection data.
6) End point method, kinetics and spectral scanning are available, as well as plates
with or without lids.
7) Xenon lamp with long lifetime which can reach to 109times.
8) With incubation function, the average temperature deviation between
wells≤0.5°C.
9) Self-checking optical path, top reading and mechanical motion.
10) With shaking function, time and speed are adjustable.
11) It supports USB data export, fast and easy to operate.
12) System multi-user hierarchical operation, easy for data management.
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FlexA-200 Microplate Reader Operation Manual Chapter 2 Features
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Chapter 2 Features
Working conditions：
Ambient temperature: 10C~40C
The relative humidity: 30%~80%(No condensation)
Power: AC100-240V 50-60Hz 2A
Parameters:
Model
Parameter
FlexA-200
Light source
Xenon flash lamp / >109flashes
Wavelength
200～1000nm
Bandwidth
≤2.5nm
Wavelength accuracy
2nm
Wavelength repeatability
0.2nm
Read-out range
0.0-4.0 OD
Linearity@450nm
R2≥0.995 , (0.0 - 3.0]
Accuracy@450nm
±(1.0% + 0.003A) , (0 - 2.0]
±2.0% , (2.0 - 2.5]
Precision@450nm
CV < 0.5% (Precision mode); CV < 1.0% (Fast mode)
Stability@450nm
< 0.005A , (0.0 - 2.0]
< 2% , (2.0 - 2.5]
Measurement speed
< 10 seconds at Fast mode (96-well plate)
< 30 seconds at Precision mode (96-well plate)
Plate shaking
Linear
Incubation range
RT+ 4°C to 45°C
Temp. uniformity
±0.5°C @ 37°C
Connections
1 B-type of USB port for PC
1 Ethernet port
2 A-type of USB ports for devices
Power requirements
DC24V 6.67A 160W
Dimension (WXDXH)
300 x 500 x 290mm
Weight (kg)
15kg
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FlexA-200 Microplate Reader Operation Manual Chapter 3 Instrument Structure
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Chapter 3 Instrument Structure
Structure
Rear cover
Touch screen
Roof cover
External Button
Plate holder
Front door
Extension module
USB Port
LAN Port
Switch
Power Socket
B-type
USB Port
Air outlet
DOMINIQUE DUTSCHER SAS

FlexA-200 Microplate Reader Operation Manual Chapter 4 Installation
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Chapter 4 Installation
1. Opening check
Each FlexA-200 is thoroughly tested before shipping, but please check again when you receive the
instrument and contact your local distributor or manufacturer if:
•The outer package is damaged
•The outer package has any obvious moisture stains
•The outer package has marks of impact
•The outer package has signs of being opened
After opening, please check the instrument and box contents.
Confirm that all ordered accessories have been included.
Check the instrument’s appearance for any damage.
2. Installation
Working condition: locate instrument on a flat dry and clean work table, keep the front side
with enough space for plate holder in and out, also keeping 15cm space for back, left and right
side to enable put or connect wires.
Working environment:
a. Clean air free from corrosion steam or smoke.
b. Temperature should be within the range of +10°C ~ +40°C.
c. Relative humidity should be within the range of 30% ~ 80% to avoid condensation.
Note: KEEP INSTRUMENT AWAY FROM DESTRUCTIVE GAS OR LIQUID!
3. Installation steps
1Place the instrument on a stable and level surface.
Note: Please DO NOT loose any screw or parts without permission, or it will cause
instrument damage and make it out of warranty.
2Connect the instrument to an appropriate power outlet using the provided power cord.
Fig. 1
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FlexA-200 Microplate Reader Operation Manual Chapter 4 Installation
─5─
Note: Attention to the interface of the power adapter, please connect it to the power
according to the direction of the above picture.
3Switch “I/O” button to “I” to turn on the instrument, the front panel will cycle through a
start-up and self-test screen.
Warning: Don’t connect instrument to power socket without ground wire.
DOMINIQUE DUTSCHER SAS