DUTSCHER ClearLine UVC/T-AR User manual

Edition 3.-6.01 – May of 2020
DUTSCHER GROUP

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3
Contents
1. About this edition of user instructions.............................................................................3
2. Safety precautions..........................................................................................................4
3. General information........................................................................................................5
4. Getting started................................................................................................................7
5. Operation........................................................................................................................8
6. Specifications................................................................................................................11
7. Care and maintenance .................................................................................................13
8. Warranty.......................................................................................................................15
9. EU Declaration of conformity........................................................................................16
1. About this edition of user instructions
The current edition of the user instructions applies to the following models and
versions of DNA/RNA UV-cleaner box:
• UVC/T-AR ........................................................................................... version V.3AD
• UVC/T-M-AR ..................................... versions V.6A02, V.6A03, V.6A04 and V.6A12
• UVT-B-AR ............................................. versions V.4AB, V.4AD, V.4AE and V.4A12
• UVT-S-AR .............................................. versions V.4AA, V.4AB, V.4A7 and V.4A04

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2. Safety precautions
Caution! Make sure you have fully read and understood the present Manual before
using the equipment. Please pay special attention to sections marked by
this symbol.
Caution! The front protective screen must be closed while the open UV lamp is
switched on. Otherwise, the operator can be exposed to a dangerous level
of UV emission.
Caution! The unit contains a source of UV radiation. Do not start the unit without the
cover of the UV recirculator. Exposure to UV light is harmful and can cause
damage to unprotected eyes and skin.
Caution! The UV-Cleaner box contains a powerful source of UV radiation, therefore,
before operating the unit, ensure every personnel working with the UV-
Cleaner box are properly protected. The operator should wear a closed-
front lab coat (fully buttoned), UV certified safety glasses and gloves, which
should overlap the lab coat or surgical gown cuffs.
GENERAL SAFETY
• The protection provided can be ineffective if the operation of the appliance does not
comply with the manufacturer's requirements.
• Save the unit from shocks and falling.
• Do not use the unit if it has visible mechanical damage.
• Store and transport the unit in a horizontal position (see package label) at ambient
temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
• Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
• Do not make modifications in design of the unit.
• When handling hazardous substances, follow the instructions contained in the safety
data sheets for the individual substances that are used and observe the relevant
accident prevention regulations.

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ELECTRICAL SAFETY
• Connect only to the mains with voltage corresponding to that on the serial number
label.
• Do not plug the unit into an ungrounded power socket, and do not use an ungrounded
extension lead.
• Ensure that the power plug is easily accessible during use.
• Disconnect the unit from the mains before moving.
• If liquid penetrates into the unit, disconnect it from the mains and have it checked by
a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
DURING OPERATION
• Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possible operation of the unit in specific
atmospheres.
• Do not operate the unit if it is faulty or has been installed incorrectly.
• Do not use outside laboratory rooms.
• Do not operate the unit without dust filters installed.
• Do not work in the box while the open UV lamp is switched on. BIOLOGICAL SAFETY
• The user is responsible to carry out appropriate decontamination if hazardous
material spills on or penetrates into the equipment.
• The user is responsible for decontamination of the unit before its decommissioning
and utilization.
3. General information
DNA/RNA UV-Cleaner boxes – UVC/T-AR, UVC/T-M-AR, UVT-B-AR and UVT-SAR
– are designed for clean operations with DNA/RNA samples. They provide protection against
contamination.
All models are bench-top type, with metal framework, glass or acrylic walls and
working surface painted with powder enamel or made of stainless steel. Box is equipped with
inlet for power cables and built-in power outlets for units inside the box. For availability of the
required characteristics, please clarify for each model separately (see 7.1 on page 11).
UV-Cleaner boxes are equipped with an open UV lamp installed in the upper hood.
UV radiation from the open lamps disinfects the working area inactivating DNA/RNA
fragments during 30 min of exposure. A digital timer controls duration of the direct UV
irradiation. A daylight lamp provides proper illumination of the working surface.

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UV-Cleaner box is equipped with a flowing bactericidal UV-cleaner-recirculator AR,
which provides constant decontamination inside the box during operation. It is recommended
for operations with DNA/RNA amplicons.
UV recirculator consists of an UV lamp (fig. 1/1), a fan and dust filters (fig. 1/2)
organized in a plastic case. Operator working in a UV-Cleaner box with a switched on UV
recirculator is not exposed to UV radiation. It allows continuous treatment of the airflow with
UV light without interrupting working process. Air circulation at a short distance from the UV
lamp combined with reflective surfaces in the air duct results in increased density of UV rays
leading to higher efficiency of disinfection. UV recirculator generates 100 volumes of PCR
cabinet per one hour of airflow exchange ensuring maximally aseptic conditions inside the
cabinet.
Box for clean operations with DNA/RNA samples with built-in recirculator is a
patented solution (patent LV13115 from 20/05/2004, Dr. biol. V. Bankovsky).
Microbiological studies at the R&D Department of Biosan led by Dr. biol. V.
Bankovsky demonstrated a high level of biosafety and efficacy of UV-cleaner box (maximal
level of contamination is 1-3 cfu per 100 litres of air).
UV-Cleaner box is designed to biologically protect the product, but not the operator,
therefore, it is not recommended to use the UV-Cleaner box for working with pathogenic
microorganisms of biosafety level BSL-II and higher, without specialized protection.
Advantages:
• UV-recirculator;
• Ozone free high density UV decontamination;
• Open UV lamp is switched off automatically in case of opening the front screen;
• Long life UV lamps (9000 h);
• Low noise level and energy consumption;
• Compact tabletop model for personal laboratories;
• Shelves for pipettes and reagents (on request)
• Table with drawer T-4 / T-4L, laboratory chest of drawers LF-1 (on request).
Figure 1. Recirculator scheme.
1. UV lamp. 2. Fan and input filter. 3. UV indicator. 4. Output filter

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4. Getting started
4.1. Unpacking. Remove packing materials carefully and retain them for future shipment or
storage of the unit. Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage. Warranty covers only the units transported in
the original package.
Caution! Due to the high weight of the unit, its unpacking and installing must
be carried out by two persons. Follow the instructions included on the
packaging.
4.2. Complete set. Package contents:
4.2.1. Standard set:
- DNA/RNA UV-cleaner box .............................................................................. 1 pce.
- Power cable (UVC/T-AR only) ......................................................................... 1pce.
- Spare fuse for control block
1
............................................................................ 1 pce.
- Spare fuse for built-in power outlet
2
................................................................ 1 pce.
- Spare dust filters ............................................................................................. 2 pcs.
- Operating instructions, declaration of conformity ........................................... 1 copy
4.2.2. Optional accessories:
- P-5, shelf for pipettes ............................................................................... on request
- F-1, shelf for reagents and samples ......................................................... on request
- PDS-250, DNA/RNA Decontamination Solution Spray, 250ml ................ on request
- T-4 / T-4L moving table for UV-Cleaner box............................................. on request
- LF-1, laboratory chest of drawers ............................................................. on request
P-5 F-1 PDS-250
T-4 T-4L LF-1
1
For information on fuse replacement, see 7.3 on page 13
2
For all models, excluding UVC/T-AR

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4.3. Setup.
- If the moving table is used, unpack it carefully and assemble according to the
enclosed assembling scheme.
- Place the unit upon stable surface. Ensure that the unit is placed on a solid, level
surface not less than 720x550 mm (1290x600 mm for UVT-S-AR model), which is
able to support its weight and the weight of equipment and materials inside, for
instance on the T-4 / T-4L table.
- Connect the power cable to the socket on the rear side of the unit, position it with
easy access to the power switch and plug.
4.4. Shelf setup. Refer to the instructions included with the shelf.
5. Operation
Note. For UVC/T-M-AR and UVT-B-AR boxes, check that the short power
cable (fig. 5/1) is connecting the fuse box and the controller top.
5.1. Connect the power plug to a grounded power socket. Turn on the Power switch:
UVC/T-AR – no switch, the unit powers up automatically;
UVC/T-M-AR, UVT-B-AR – on the right side of the unit, behind the rear wall (fig. 5/3);
UVT-S-AR – on the left side of the unit, behind the rear wall (fig. 6/1).
5.2. UV exposition of the working place.
Caution! The front protective screen must be closed while the open UV lamp is
switched on. Otherwise, the operator can be exposed to a dangerous level
of UV emission.
5.2.1. Turn ON the switch 2(fig. 2/2) on the control panel (fig. 3/1). This switches on the UV
recirculator inside the unit (fig. 3/2) and the open UV-lamp exposition timer. The UV
recirculator will operate all the time until the switch 2is turned OFF.
Figure 2. Control panel

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Note. Open UV lamp operation can be checked with the visible light lamp switched
off (switch 1is OFF). Use the indicator in the centre of recirculator cover (fig.
1/3) to check operation of UV lamp inside the recirculator. If indicator lights
from inside while switch 2is ON, then the UV lamp is functioning.
5.2.2. Use the timer +and -keys (fig. 2/4) to increase and decrease the time (SETTING
TIME) of direct UV light exposition of the working place, with 1 min increment.
Pressing down and holding the button for more than 2 seconds increases the
increment. Recommended time of exposition is two sessions of 30 minutes, once
after switching on the cabinet, once before operations inside.
Note. When using the P-5 and F-1 shelves, extend the exposition time to two
sessions of 45 minutes.
5.2.3. Press the Start key (fig. 2/3), the UV lamp will be turned on automatically and the
timer will start counting the exposition time. Timer indicator shows actual time: until 1
hour - in minutes and seconds (mm:ss), after 1 hour - in hours and minutes (hh:mm).
After reaching the set time the timer will automatically turn off the open UV lamp.
5.2.4. The open UV lamp can be switched off by pressing the Stop/Reset key (fig. 2/5). The
set time of exposition will be saved in the memory. The set time will not be saved after
the complete turning off the unit.
5.2.5. If the set time of open UV light exposition is 0:00, pressing the Start key will make
the unit operate continuously during 24 hrs or until the Stop/Reset key is pressed.
5.3. The box is ready for operations. Work in the box.
Note. Opening the front protective screen will switch off the open UV lamp
automatically, but the timer will continue counting the exposition time.
5.3.1. Turn ON switch 1(fig. 2/1) for lighting of the working place. This turns ON the
luminescent (visible light) lamp inside the cabinet (fig. 3/3).
5.3.2. Lift the front protective screen (fig. 3/5) up for work in the cabinet. Models have
different maximum opening heights (fig. 3/8), as listed in the Specifications section,
and may be raised to the following positions:
• Model UVC/T-AR has a single opening height;
• Models UVC/T-M-AR, UVT-B-AR and UVT-S-AR has three protective screen
positions – one-third, two-thirds and full opening height.
•
Note. Do not block the recirculator openings (fig. 3/2)!

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5.3.3. To use electric devices inside the cabinet, pull their power cable through the inlet and
close the flap (fig. 3/6) or connect the power cable to a built-in mains socket (fig. 3/7,
models with mains socket).
Warning! Overall consumed power of devices connected through internal mains
sockets should not exceed 1000 W for 230V, or 600 W for 100– 120
V.
5.4. After the task is done, close the front protective screen.
5.5. After finishing the operation turn OFF switch 2and switch 1. Turn off the Power
switch, if present. Disconnect the power cable from the mains.
UVC/T-AR, UVC/T-M-AR, UVT-B-AR UVT-S-AR
Figure 3. DNA/RNA UV box, frontal view:
1. Control panel.
2. Recirculator openings.
3. Daylight lamp and open UV lamp(s).
4. Upper front panel.
5. Movable front protective screen (opened).
6. Cable inlet.
7. Power outlet(s) (not in UVC/T-AR).
8. Opening height.

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6. Specifications
The unit is designed for operation in cold rooms and closed laboratory rooms at
ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum
relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative
humidity at 40°C.
The manufacturer is committed to a continuous programm of improvement and
reserves the right to alter design and specifications of the equipment without additional
notice.
Model
UVC/T
-
AR
Compact
UVC/T
-
M
-
AR
Compact
UVT
-
B
-
AR
Compact
UVT
-
S
-
AR
Double
Back panel
PMMA
Steel
Side panels Glass
Coated steel
Glass
Front panel and screen Glass
Working surface Coated steel Steel
Open UV lamp 1хTUV 25W G13 UV-C 2хTUV 30W G13 UV-C
UV intensity 15 mW/cm
2
/s
Radiation type Ultraviolet light (λ=253.7 nm), no ozone
Time setting of direct
UV exposure
Digital timer, 1 minute - 24 hours / non-stop (increment 1 minute)
UV recirculator lamp 1хTUV 25W G13 UV-C 1хTUV 30W G13 UV-C
Visible light lamp 1хTLD 15W G-13 1хTLD 30W G-13
Thickness of side panels 4 mm 4 mm 2 mm 4 mm
Thickness of upper front panel 8 mm
Thickness of protective screen 8 mm 4 mm 4 mm 5 mm
Optical transparency 92% 95%
UV protection > 99.90% > 96%
Noise level < 45 dB < 47 dB
Working surface 490x645 mm 500x1210 mm
Opening height 165 mm 190 mm (max 210 mm) 190 mm (max 220 mm)
Opening levels Full height 1/3, 2/3 and full height
Power inside the box (see
7.1
)
Cable inlet Cable inlet and 1 socket
1
Cable inlet and 3 sockets
1
Operating current 100–240 V, 50/60 Hz
Power consumption 67 W 135 W
Dimensions 720x535x555
mm
700x580x555 mm 1250 x 600 x 590 mm
Weight (net/gross)
2
23 / 33 kg 28.8 / 39 kg 31.2 / 42 kg 58 / 68.5 kg
Laboratory table T-4 T-4L
1
Warning! Overall consumed power of devices connected through internal mains sockets should not
exceed 1000 W for 230V, or 600 W for 100–120 V
2
Accurate within ±10%

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6.1. Used materials.
• PMMA – polymethylmethacrilate (acrylic) glass, Altuglas
®
EX.
• Glass – clear film (0.1 mm) coated glass, Euroglas
®
.
• Steel – stainless polished steel.
• Coated steel – white enamel powder coated steel.
6.2. Cabinet dimensions. Outer and inner dimensions, opening heights.
UVC/T-AR UVC/T-M-AR, UVT-B-AR
UVT-S-AR
Warning! Overall consumed power of devices connected through internal mains
sockets should not exceed 1000 W for 230V, or 600 W for 100– 120
V.
6.2. To inquire about or order the optional accessories, contact your local representative.

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7. Care and maintenance
7.1. If the unit requires maintenance, disconnect the unit from the mains and contact your
local representative.
7.2. All maintenance and repair operations must be performed only by qualified and
specially trained personnel.
7.3. Fuse replacement.
7.3.1. Fuse for the control box. Disconnect the unit from electric circuit. Remove power cable
from its socket on the rear side of the unit. Open the fuse holder by pulling out the
holder (fig. 4, compact models) or by screwing off the holder lid marked FU2 (fig. 6,
model UVT-S-AR). Check the fuse and replace if necessary, М3.15 A (type M- time
lag: Medium).
7.3.2. Fuse for the mains outlets (for models with built-in mains outlets). Disconnect the unit
from electric circuit. Open the fuse holder by screwing off the holder lid (fig. 5/2 or fig
6, FU1). Check thefuse and replace if necessary, М5.0 A(type M- time lag: Medium).
Figure 4. Figure 5 Figure 6
7.4. UV lamp replacement. Average lifetime of UV lamps supplied is 9000 hrs. Replacement
is necessary after lamp stops functioning or at the end of manufacturer specified
lifetime.
Note. Open UV lamp operation can be checked with the visible light lamp switched
off (switch 1is OFF). Use the indicator in the centre of recirculator cover (fig.
1/3) to check operation of UV lamp inside the recirculator. If indicator lights
from inside while switch 2is ON, then the UV lamp is functioning.
7.5. Dust filter replacement. The dust filters (fig. 3/2) on either end of the UV recirculator
with the hidden UV lamp should be checked monthly and cleaned or replaced when
they become clogged. To check, replace or clean the filters, simply unclip the covers,
fit a new filter if necessary or rinse current filters in water, dry and place back. Clip
covers back in place.

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7.6. Cleaning and decontamination. Disconnect the unit from the mains before cleaning.
Caution! Do not let liquid get into the control box.
7.6.1. Model UVC/T-AR and shelves P-5 and F-1, full cleaning. Transparent panels and
shelves are made of acrylic glass (polymethylmethacrylate Altuglas
®
EX) and are prone to
scratches and optical transmission capacity decrease if improperly cleaned. Use mild soap
and water with a soft cloth or sponge for cleaning the panels. Wipe excess water from inside
and outside the unit with an absorbent soft cloth or sponge. For decontamination, it is
recommended to use a special DNA/RNA removing solution (e.g. PDS-250). After washing
the inside parts of the box it is necessary to rub them dry.
Caution! Never use organic solvent based compounds, pure alcohol, alcoholcontaining
cleaners (more than 20%) or ammonia containing cleaners for acrylic
glass. Do not use abrasives. The table below shows the interaction of
acrylic glass with ethyl alcohol and other solutions.
Solution
Interaction with acrylic glass
Biosan PDS-250 No effect
DNA-Exitus Plus™ No effect
RNase-Exitus Plus™ No effect
Hydrogen peroxide H
2
O
2
6% No effect
Ethanol C
2
H
2
OH ≤20% No effect
Ethanol C
2
H
2
OH > 20% Increasing effect. Do not use.
Note. Crazing is a normal process for acrylic glass panels exposed to open UV light.
Crazing will occur over time. Crazing may occur within the warranty
period and is regarded as normal wear and not covered by the
warranty. Acrylic glass panels can be replaced.
7.6.2. Models UVC/T-M-AR, UVT-B-AR and UVT-S-AR, external cleaning. Glass panels on
the outside are coated in 4 mil clear film for UV protection. Film manufacturer
recommends using a soft sponge or cloth with common washing solution for glass,
removing excess and wiping dry. Do not use ethanol or other organic solvents.
7.6.3. Models UVC/T-M-AR, UVT-B-AR and UVT-S-AR, internal cleaning and
decontamination. The following substances are recommend to use for
decontamination: 75% ethanol, sodium hypochlorite solution, DNA/RNA removing
solution (e.g. Biosan
PDS-250). After washing the inside parts of the box it is necessary to wipe them dry.

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8. Warranty
8.1. The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and
transportation instructions.
8.2. The warranted service life of the unit from the date of its delivery to the Customer is 12
months.
8.3. Warranty covers only the units transported in the original package.
8.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
8.5. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model Serial number Date of sale
UVC/T
-
AR, UVT
-
B
-
AR,
UVC/T-M-AR, UVT-S-AR,
DNA/RNA UV-cleaner box

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9. EU Declaration of conformity