EDAN H50 User manual

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About this Manual
P/N: 01.54.457881
MPN: 01.54.457881015
Release Date: April, 2019
© Copyright EDAN INSTRUMENTS, INC. 2017-2019. All rights reserved
Statement
This manual will help you to better understand the operation and maintenance of the product. It
is reminded that the product should be used strictly in compliance with this manual. User
operation failing to comply with this manual may result in malfunction or accident for which
EDAN INSTRUMENTS, INC. (Hereinafter called EDAN) can’t be held liable.
EDAN owns the copyrights of this manual. Without prior written consent of EDAN, any
materials contained in this manual shall not be photocopied, reproduced or translated into other
languages.
Materials protected by the copyright law, including but not limited to confidential information
such as technical information and patent information contained in this manual, shall not be
disclosed to any irrelevant third party.
The user shall understand that nothing in this manual grants him, expressly or implicitly, any
right or license to use any of the intellectual properties of EDAN.
EDAN holds the rights to modify, update, and ultimately explain this manual.
Responsibility of the Manufacturer
EDAN only considers itself responsible for any effect on safety, reliability and performance of
the equipment if:
Assembly operations, extensions, re-adjustments, modifications or repairs are carried out by
persons authorized by EDAN, and
The electrical installation of the relevant environment complies with national standards, and
The instrument is used in accordance with the instructions for use.
Upon request, EDAN may provide necessary circuit diagrams, and other information to help
qualified technicians maintain and repair some parts, which EDAN may configure as user
serviceable.
Terms Used in this Manual
This guide is designed to give key concepts on safety precautions.
WARNING
A WARNING label advises against certain actions or situations that could result in personal
injury or death.
CAUTION
A CAUTION label advises against actions or situations that could damage equipment, produce
inaccurate data, or invalidate a procedure.
NOTE
A NOTE provides useful information regarding a function or a procedure

- 1 -
A Table of Contents
Chapter 1 Safety Guidance ........................................................................................................... - 1 -
1.1 Indications for Use/Intended Use .......................................................................................... - 1 -
1.2 Warnings and Cautions .......................................................................................................... - 1 -
1.2.1 Safety Warnings .............................................................................................................. - 1 -
1.2.2 Safety Cautions ............................................................................................................... - 3 -
1.3 Symbols and Definitions ....................................................................................................... - 4 -
Chapter 2 General Overview ....................................................................................................... - 6 -
2.1 Introduction ............................................................................................................................ - 6 -
2.2 Main Parts ............................................................................................................................... - 7 -
2.2.1 Front cover....................................................................................................................... - 7 -
2.2.2 Fluidic part....................................................................................................................... - 7 -
2.2.3 Reagent area .................................................................................................................... - 8 -
2.2.4 Connection board ............................................................................................................ - 9 -
2.2.5 External power supply Adapter .................................................................................... - 10 -
2.2.6 Printer............................................................................................................................. - 10 -
2.2.7 External Barcode reader ............................................................................................... - 10 -
2.3 Configuration ....................................................................................................................... - 11 -
2.3.1 Standard Configuration ................................................................................................. - 11 -
2.3.2 Options ........................................................................................................................... - 11 -
Chapter 3 Measurement Principle ............................................................................................ - 12 -
3.1 WBC, RBC and PLT Measurement Principle.................................................................... - 12 -
3.2 Five-part Diff Measurement Principle ................................................................................ - 13 -
3.3 HGB Measurement Principle .............................................................................................. - 14 -
3.4 MCV and MPV Measurement ........................................................................................... - 14 -
3.5 Equations for Calculated Parameters .................................................................................. - 14 -
3.5.1 Calculation of WBC-Related Parameters .................................................................... - 14 -
3.5.2 Calculation of RBC-Related Parameters ..................................................................... - 15 -
3.5.3 Calculation of PLT-Related Parameters ...................................................................... - 15 -
Chapter 4 Installation Guidance ............................................................................................... - 17 -
4.1 Installation Environment ..................................................................................................... - 17 -
4.2 Unpacking............................................................................................................................. - 17 -
4.2.1 Unpacking Inspection ................................................................................................... - 17 -
4.2.2 Visual check .................................................................................................................. - 17 -
4.3 Installation ............................................................................................................................ - 18 -
4.4 User Login ............................................................................................................................ - 20 -
Chapter 5 Software Introduction .............................................................................................. - 21 -

- 2 -
5.1 Toolbar Introduction ............................................................................................................ - 21 -
5.2 Common Buttons Introduction ............................................................................................ - 21 -
5.3 Software Screen Introduction .............................................................................................. - 22 -
Chapter 6 Reagents Management ............................................................................................. - 23 -
6.1 Diluent/ Lyse/Cleaner Management ................................................................................... - 23 -
6.2 Waste Management.............................................................................................................. - 24 -
Chapter 7 Startup ........................................................................................................................ - 25 -
Chapter 8 QC ................................................................................................................................ - 26 -
Chapter 9 Calibration ................................................................................................................. - 29 -
Chapter 10 Repeatability ............................................................................................................ - 31 -
Chapter 11 Sample Test .............................................................................................................. - 32 -
11.1 Sample Preparation ............................................................................................................ - 32 -
11.2 Run Sample ........................................................................................................................ - 32 -
11.3 Viewing Test Results ......................................................................................................... - 34 -
11.4 Searching Test Results....................................................................................................... - 34 -
11.5 Re-edit Patient Data ........................................................................................................... - 35 -
Chapter 12 Flags .......................................................................................................................... - 36 -
12.1 Results Flags ...................................................................................................................... - 36 -
12.2 System Alerts ..................................................................................................................... - 37 -
12.3 Morphologic Alarms .......................................................................................................... - 38 -
12.3.1 WBC Histogram Alarm .............................................................................................. - 38 -
12.3.2 RBC Histogram Alarm ............................................................................................... - 39 -
12.3.3 PLT Histogram Alarm ................................................................................................ - 40 -
12.3.4 WBC Scattergram Alarm............................................................................................ - 41 -
12.4 Pathological Alarms........................................................................................................... - 42 -
12.4.1WBC Pathological Alarms .......................................................................................... - 42 -
12.4.2 RBC Pathological Alarms .......................................................................................... - 43 -
12.4.3 PLT Pathological Alarms ........................................................................................... - 43 -
Chapter 13 Service ....................................................................................................................... - 44 -
13.1 System Initialization .......................................................................................................... - 44 -
13.2 Event Logs .......................................................................................................................... - 44 -
13.3 Error Logs........................................................................................................................... - 45 -
13.4 Backup & Restore .............................................................................................................. - 45 -
13.5 Settings ............................................................................................................................... - 45 -
13.5.1 Lab Parameters Setting ............................................................................................... - 46 -
13.5.2 Date/Time Setting ....................................................................................................... - 50 -

- 3 -
13.5.3 Automatic Cycles Setting ........................................................................................... - 50 -
13.5.4 Printer Setting .............................................................................................................. - 51 -
13.5.5 Communication Setting .............................................................................................. - 52 -
13.5.6 Users Management Setting ......................................................................................... - 53 -
13.5.7 Software Update Management ................................................................................... - 54 -
13.6 Troubleshooting ................................................................................................................. - 55 -
13.6.1 Troubleshooting via Software .................................................................................... - 55 -
13.6.2 Parameters Troubleshooting ....................................................................................... - 58 -
13.6.3 Mechanical Problems Troubleshooting ..................................................................... - 59 -
13.7 Maintenance ....................................................................................................................... - 60 -
13.7.1 Automatic Bleach Cleaning Maintenance ................................................................. - 60 -
13.7.2 Regular Maintenance .................................................................................................. - 60 -
13.7.3 Cleaning and Disinfecting the Analyzer .................................................................... - 61 -
Chapter 14 Logout and Shutdown ............................................................................................ - 63 -
14.1 Logout ................................................................................................................................. - 63 -
14.2 Shutdown ............................................................................................................................ - 63 -
Chapter 15 Warranty and Service ............................................................................................ - 64 -
15.1 Warranty ............................................................................................................................. - 64 -
15.2 Contact Information ........................................................................................................... - 64 -
Appendix 1 Specifications ........................................................................................................... - 65 -
A1.1 Environment Requirements .............................................................................................. - 65 -
A1.2 Analyzer Specifications .................................................................................................... - 65 -
A1.3 Performance Specifications .............................................................................................. - 65 -
A1.4 Power Adapter ................................................................................................................... - 65 -
Appendix 2 Analytical Performance ......................................................................................... - 66 -
A2.1 Linear Range ..................................................................................................................... - 66 -
A2.2 Background ....................................................................................................................... - 66 -
A2.3 Precision ............................................................................................................................ - 66 -
A2.4 Carry-over.......................................................................................................................... - 66 -
A2.5 Accuracy ............................................................................................................................ - 67 -
A2.6 Measurement Range ......................................................................................................... - 67 -
Appendix 4 EMC Information ................................................................................................... - 68 -
Appendix 3 Safety Specifications ............................................................................................... - 71 -
Appendix 5 Glossary .................................................................................................................... - 72 -
Appendix 6 User Access .............................................................................................................. - 73 -

H50/H51 Hematology Analyzer User Manual Safety Guidance
- 1 -
Chapter 1 Safety Guidance
1.1 Indications for Use/Intended Use
H50/H51 Hematology Analyzer is intended to qualitatively and quantitatively measure blood
cells and provide measurement results. It is intended for some routine blood tests or physical
examination in outpatient department or ward of a hospital. H50/H51 Hematology Analyzer
supports to test whole blood samples. Human body fluid, human serum or plasma, and animal
blood are not tested by the analyzer.
The sample must be collected in a tube with K2EDTA.
H50/H51 Hematology Analyzer should be operated by trained and qualified medical personnel.
1.2 Warnings and Cautions
1.2.1 Safety Warnings
NOTE:
The reliability of the analyzer and the safety of operators are considered during product
design and production. The following safety and preventive measures should be carried
out:
WARNING
Safety Warnings
1. The analyzer is not intended for treatment.
2. The analyzer is not intended for home use.
3. Do not use the analyzer if it is damaged or defective.
4. The analyzer should be installed by a qualified service engineer. Do not try to
access into the interior of the analyzer. Only authorized service personnel are
authorized to disassemble or maintain the analyzer.
5. To avoid electrical shock, never modify the analyzer’s AC power circuits.
6. The analyzer is intended for use only by trained technologists, nurses, physicians
and therapists. Operators should be familiar with the contents of this user manual
before operation.
7. The results given by the system should be examined based on the overall clinical
condition of the patient, and should not be a substitute for regular checking and
examination.
8. To ensure grounding reliability, only connect the system to a hospital-grade power
receptacle.

H50/H51 Hematology Analyzer User Manual Safety Guidance
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WARNING
9. Connect the analyzer to a grounded socket and make certain that the power supply
meets the requirements specified in the user manual.
10. Do not exceed the maximum permitted load when using multiple portable socket-
outlets to supply the system.
11. SHOCK HAZARD - Do not attempt to connect or disconnect a power cord with wet
hands. Make certain that your hands are clean and dry before touching a power cord.
12. If you have any questions about the power adaptor or the power cord prior to using,
consult EDAN or its authorized distributors for service.
13. The analyzer is not waterproof. Do not use it in locations where water or any liquid
leakage may occur.
14. To avoid the possibility of electrostatic shock and damage to the system, avoid using
aerosol spray cleansers on the analyzer screen.
15. EXPLOSION HAZARD – The analyzer is not suitable for use in the presence of a
flammable anesthetic mixture with oxygen or other flammable compounds.
16. To avoid electrical shock, never use the system in altitude exceeding 3,000 meters
above sea level.
17. LASER RADIATION – The bar code scanner is a laser product. Do not view beam
generated by the scanner with eyes directly.
18. Periodically have the safety of the analyzer checked by a qualified service engineer.
19. Only accessories supplied or recommended by the manufacturer should be used.
Otherwise, performance and electric shock protection can‘t be guaranteed.
20. Blood samples should be collected according to proper medical guidelines which
contains collection details, such as selection of blood collection site, collection
procedures, sample handling, etc. Sterile techniques should be followed to prevent
the blood collection site from being contaminated.
21. Handle blood samples and collection devices with care, and wear approved
protective gloves to avoid direct contact with samples.
22. To avoid electrical shock and damage to the system, turn off the analyzer and
disconnect the analyzer from the AC power source before cleaning and disinfecting.
23. To avoid being injured, never touch the sampling needle directly with hands.
24. The system is for in vitro diagnostic use only.
25. Perform quality control (QC) tests to make sure that the system works smoothly.
26. The sample collection tube should only be used a single time.
27. The sample is contained in the sample tubes, so sample tubes need to be
disposed of as bio-hazardous waste, complying with local regulatory guidelines.

H50/H51 Hematology Analyzer User Manual Safety Guidance
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WARNING
28. The analyzer needs to be disposed of according to local regulations after their useful
lives. Alternatively, they can be returned to the dealer or the manufacturer for
recycling or proper disposal. Please delete or back up safety data contained in the
analyzer before disposing of it.
29. Misuse of electrical equipment may cause electrocution, burns, fire and other
hazards.
30. Due to the use and the environment of this equipment, all parts and surfaces of
H50/H51 Analyzer are potentially infective. Wearing gloves when operating or
maintaining the analyzer and hands washing after work are essential.
31. Only reagents including Lyse, Cleaner, Diluent, Bleach, Hematology Controls and
Hematology Calibrator provided by EDAN or its authorized distributors should be
used. If other reagents are used, Edan will not assure the accuracy of test results
and will not be responsible for the analyzer’s maintenance and repair caused by the
reagents.
32. Don't position the analyzer in some places where it's difficult to disconnect the AC
plug.
1.2.2 Safety Cautions
CAUTION
1. Do not use the analyzer in a dusty environment with bad ventilation or in the
presence of corrosives.
2. Make sure that the time and date of the system are correct.
3. The system is intended to analyze whole blood samples. Never use it to analyze
serum or plasma.
4. If there are clots or bubbles in the blood sample, discard it and collect samples again.
5. Transport, store and use the analyzer, diluent, lyse, cleaner, Hematology Controls
and Hematology Calibrator according to the corresponding user manual.
6. Connect the analyzer with the peripherals recommended by EDAN.
7. Maintain the system as described in the user manual to avoid potential damage.
8. Do not clean the analyzer and accessories with abrasive fabric.
9. Do not immerse the analyzer into liquid under any circumstances.
10. Extreme humidity may affect test results. A relative humidity greater than 80% may
cause inaccurate results.
11. Use this system at a temperature between 18 °C and 32 °C. Outside this range, the
system may produce inaccurate results.
12. The cable tie must be removed before the analyzer is firstly turned on after
completion of its first installation. If not, the analyzer’s operation might be affected or
even worse the analyzer might be damaged.

H50/H51 Hematology Analyzer User Manual Safety Guidance
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CAUTION
13. Make sure that there is no intense electromagnetic interference source around the
analyzer, such as radio transmitters, mobile phones etc. Attention: large medical
electrical equipment such as electrosurgical equipment, radiological equipment and
magnetic resonance imaging equipment is likely to bring electromagnetic
interference.
1.3 Symbols and Definitions
No.
Symbol
Description
1.
Consult instructions for use
2.
Warning, biological hazard
(Background color-
yellow; symbol and outline-
black)
3.
Recycle
4.
On/Off button
5.
Network port
6.
Serial port
7. USB (Universal Serial Bus) connection
8.
In vitro diagnostic medical device
9.
Disposal Method
10.
Manufacturer
11.
Date of manufacture
12.
Temperature limit

H50/H51 Hematology Analyzer User Manual Safety Guidance
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13.
Use-by Date
14.
Serial number
15.
Caution: Federal (U.S.) Law restricts this device to
sale by or on the order of a physician.
16.
Equipotential grounding
17.
Current: Direct
18.
Warning
(Background: yellow
Symbol and outline: black)
19.
Batch code
20.
Hand Crush/Pinch Point
(Background: yellow
Symbol and outline: black)
21.
Caution
22.
The symbol indicates that the device complies with
the European Council Directive 98/79/EC
concerning medical devices.

H50/H51 Hematology Analyzer User Manual Measurement Principle
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Chapter 2 General Overview
2.1 Introduction
H50/H51 Analyzer is an automated analyzer performing hematological analysis on whole blood
collected on tubes containing K2EDTA as anticoagulant. All parameters determined by the
analyzer are listed as follows:
White blood cell parameters:
Determination
Symbol
Description
WBC
Total Count
Measured
LYM%/#
Lymphocyte percent and absolute value
Measured/Calculated
MON%/#
Monocyte percent and absolute value
Measured/Calculated
NEU%/#
Neutrophil percent and absolute value
Measured/Calculated
EOS%/#
Eosinophil percent and absolute value
Measured/Calculated
BAS%/#
Basophils percent and absolute value
Measured/Calculated
ALY*(%/#)
Abnormal Lymphocyte percent and absolute value
Calculated
IMM*(%/#)
Immature Monocyte percent and absolute value
Calculated
Red blood cell parameters:
Determination
Symbol
Description
RBC
Total count
Measured
HGB
Hemoglobin
Measured
HCT
Hematocrit
Calculated
MCV
Mean Cell Volume
Measured
MCH
Mean Cell Hemoglobin
Calculated
MCHC
Mean Cell Hemoglobin Concentration
Calculated
RDW CV
Red Blood Cells Distribution Width-CV
Calculated
RDW SD
Red Blood Cell Distribution Width-SD
Calculated
Platelet parameters:
Determination
Symbol
Description
PLT
Total count
Measured
MPV
Mean Platelet Volume
Measured
PCT
Platelet Hematocrit
Calculated
PDW
Platelets Distribution Width
Calculated
PLCR
Large Platelets Cell Ratio
Calculated
PLCC
Large Platelets Cell Count
Calculated
NOTE:
9 Result is displayed and printed before the end of the analysis cycle.
9 Parameters followed with (*) are RUO (Research Use Only).

H50/H51 Hematology Analyzer User Manual Measurement Principle
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2.2 Main Parts
H50/H51 Analyzer consists of front cover, fluidic part, reagent area, connection board, external
power supply adapter, printer (optional) and external Barcode reader.
2.2.1 Front cover
The front cover consists of the five elements listed below:
1. Touch and display screen (10.4”)
2. ON/OFF button
3. Start key
4. USB port connection
2.2.2 Fluidic part
The fluidic part is located on the right side of the instrument directly accessible after opening of
the fluidic door. The fluidic part consists of the six elements listed below:
1. Sampling module
2. Syringe module
3. Syringe valve module
4. Counting module
5. Counting valve module
6. Optic Bench
1
3
2
4

H50/H51 Hematology Analyzer User Manual Measurement Principle
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2.2.3 Reagent area
The reagent area is located on the left side of the instrument directly accessible after opening of
the reagent door. The reagent area is dedicated for lyse & cleaner reagents.
1
2
3
4
5
6

H50/H51 Hematology Analyzer User Manual Measurement Principle
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2.2.4 Connection board
The connection board is located at the back of the instrument and allows different types of
connections described below.
4 USB Ports
Serial Link RS232
Not Used
P/S 24V
TCP/IP Port

H50/H51 Hematology Analyzer User Manual Measurement Principle
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2.2.5 External power supply Adapter
H50/H51 Analyzer is supplied with an external power supply adapter.
100-240 VAC; 50/60 Hz; Single phase with ground
NOTE:
If the power cord provided with the instrument needs to be replaced, the new cord must
be in compliance with the local regulation and the instrument specification in matter of
consumption.
2.2.6 Printer
H50/H51 Analyzer is not delivered with the printer. The instrument is equipped already with
PCL3 & PCL6 standard printer drivers which cover a large selection of printer models, such as
HP Laserjet Professional P1106.
2.2.7 External Barcode reader
An external barcode reader (model OPTICON - C37) can be connected to an USB port of
H50/H51 Analyzer. It only allows entering the following information automatically:
¾ Sample identification SID
¾ Reagent identification (LYSE, CLEANER and DILUENT)
¾ QC and calibration lot number

H50/H51 Hematology Analyzer User Manual Measurement Principle
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2.3 Configuration
2.3.1 Standard Configuration
x 1 analyzer
x 1 power cord
x 1 power supply adapter
x 1 flat screwdriver
x 1 user manual
x 1 certificate of approval
x 1 diluent pick up tubing
x 1 waste tubing
x 1 waste container (10L)
x 1 quick reference card
x 1 USB Barcode Reader
x 1 Packing List
2.3.2 Options
x 1 USB Printer
x 1 USB Keyboard
NOTE:
The following table lists the differences between H50 Hematology Analyzer and H51
Hematology Analyzer.
Model
USB Keyboard
Software (English Version)
Software (Other Languages)
H50
Optional Standard Optional
H51
Not Applicable
Standard Optional

H50/H51 Hematology Analyzer User Manual Measurement Principle
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Chapter 3 Measurement Principle
3.1 WBC, RBC and PLT Measurement Principle
In accordance with electrical impedance method based on Coulter Principle, WBC total counts is
measured in the WBC counting chamber , RBC total counts and PLT total counts in the RBC
counting chamber. This method is based on the modification of the impedance of a calibrated
aperture soaking in an electrolyte and going through a constant current delivered by two
electrodes located on both sides of the aperture. A vacuum applied on a side of the aperture
allows the cells passage. Cells oppose their physical volume to the current passage. A voltage
impulse is registered at the electrodes terminal. The height of this impulse is proportional to the
cell volume.

H50/H51 Hematology Analyzer User Manual Measurement Principle
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3.2 Five-part Diff Measurement Principle
The principle of this technology is based on the introduction of the sample solution and a
sheathing liquid (Diluent) at the same in the flow cell. Thus, the sheathing liquid drives the
sample flow straight across the cuvette through the detection area.
The main advantages of the measurement principle are:
1) High level of reliability of the optical adjustment
2) Only two measurement axes for five parameters
3) High resolution matrix
4) Low level of contamination between two measurements
5) Low Reagents consumption

WBC 5 differential percentages are obtained by optic measurements. The measured pulses on the
two optical channels are displayed on DIF Plot ALL (Y axis) and FSC (X axis). Each dot on the
DIF Plot represents the height in ALL and FSC of at least one pulse.


H50/H51 Hematology Analyzer User Manual Measurement Principle
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3.3 HGB Measurement Principle
3.4 MCV and MPV Measurement
MCV is the average volume of individual erythrocytes. Based on RBC histogram, the analyzer
measure MCV directly.
MPV is the average volume of individual platelets. Based on PLT histogram, the analyzer
measure MPV directly.
3.5 Equations for Calculated Parameters
3.5.1 Calculation of WBC-Related Parameters
1) Lymphocyte absolute value (LYM#) is made from LYM% and WBC total counts by formula
below:
LYM# = LYM %  WBC (total counts)
2) Monocyte absolute value (MON#) is made from MON% and WBC total counts by formula
below:
MON# = MON%  WBC (total counts)
3) Neutrophil absolute value (NEU#) is made from NEU% and WBC total counts by formula
below:
NEU# = NEU%  WBC (total counts)
4) Eosinophil absolute value (EOS#) is made from EOS% and WBC total counts by formula
below:
EOS# = EOS%  WBC (total counts)
5) Basophils absolute value (BAS#) is made from BAS% and WBC total counts by formula
below:
BAS# = BAS%  WBC (total counts)
6) ALY% is the percentage of the LYM based on a fixed threshold in the lymphocyte’s
population area
The HGB measurement is directly done in the WBC
chamber, by spectrophotometer (545 nm). H
GB
is
detected by formation of oxyhemoglobin.
HGB blank check is performed at each STARTUP
cycle. HGB Blank measurement
is done also at the
beginning of each analysis cycle.
By comparison of
the two values, it is possible to follow potential
evolution of the blank value in order to warn the user
if necessary.

H50/H51 Hematology Analyzer User Manual Measurement Principle
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7) Abnormal lymphocyte absolute value (ALY#) is made from ALY% and lymphocyte absolute
value by formula below:
ALY# = ALY% x LYM#
8) IMM% is the number of elements in the IMM population area divided by sum of elements
in DIF scattergram including IMM
9) Immature monocyte absolute value (IMM#) is made from IMM% and WBC total counts by
formula below:
IMM# = IMM% x WBC (total counts)
3.5.2 Calculation of RBC-Related Parameters
1) Hematocrit (HCT) is measured by integration of the volume of the red blood cells which
flow in the RBC counting chamber aperture.
HCT= ୖ୆େ୶୑େ୚
ଵ଴
Where HCT is expressed in %, RBC is expressed in 1012/L and MCV is expressed in fL.
ʹሻ Mean Corpuscular Hemoglobin (MCH) calculation is obtained from HGB and RBC by the
following formula 
MCH = ୌୋ୆
ୖ୆େ
Where MCH is expressed in pg, HGB is expressed in g/L and RBC is expressed in 1012/L.
3) Mean Corpuscular Hemoglobin Concentration (MCHC) is made from HGB and HCT by the
formula below:
MCHC = ୌୋ୆୶ଵ଴଴
ୌେ୘ 
Where MCHC is expressed in g/L, HGB is expressed in g/L and HCT is expressed in %.
4) RDW CV parameter is derived from the RBC histogram.
5) RDW SD parameter is calculated on curve distribution (defined as curve width at 20% of
peak)
3.5.3 Calculation of PLT-Related Parameters
1) Thrombocrit (PCT) is made from PLT and MPV by formula below:
PCT = ୔୐୘୶୑୔୚
ଵ଴଴଴଴
Where PCT is expressed in %, PLT is expressed in 109/L and MPV is expressed in fL.