Elitech AEROSPRAY CYTOLOGY 7522 User manual

USER’S
MANUAL
AEROSPRAY®
CYTOLOGY
MODEL 7522
Slide Stainer/Cytocentrifuge

2
AEROSPRAY®
Cytology Slide Stainer/Cytocentrifuge
MODEL 7522
USER’S MANUAL
57-0197-01A
(Last update 03/23/2020)

3
©2020 ELITechGroup Inc. All rights reserved.
Printed in the United States of America. No part of this publication may be reproduced, transmitted, transcribed,
stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means
whatsoever, without the prior express written permission of ELITechGroup Inc.
Aerospray®, Cytopro® are trademarks of ELITechGroup Inc.
Other trade names used in this manual are trademarks of their respective owners, used here for information
only.
ELITechGroup Inc. makes no express or implied warranty regarding this manual, its quality, performance, or
appropriate use regarding any type of specic procedure. ELITechGroup Inc. may modify this manual without
notice and without implying any obligation or liability on the part of the company.
ELITechGroup Inc.
370 West 1700 South
Logan, Utah 84321-8212 USA

Table of Contents
Section 1: Introduction
1.1 Instrument Overview 7
Using This Manual 7
Functional Description 8
Key Features 8
Intended Use 9
1.2 Instrument Description 14
Figure 1: Front and Right-Side Panels 14
Figure 2: Front Panel and Touchscreen 15
Figure 3: Rear Panel 15
Figure 4: Stainer Bowl Components 16
Figure 5: Nozzle Diagram 16
Barcode Reader 18
Figure 6: Barcode Reader 18
Other Needed Items 18
1.3 Touchscreen and User Interface 19
Section 2: Setup and Preparation for Operation
2.1 Instrument Setup 22
Unpacking and Installing the Stainer 22
Connecting the Drain Tube and Waste Container 22
Connecting Power 23
Installing Standard 500 mL Reagent Bottles for the First Time 24
Installing the Barcode Reader 25
Preparing the Stainer for Operation 25
Priming Procedures 25
The Clean Cycle 27
Performing Tests 28
Reagent Level Monitoring 28
Section 3: Controlling and Customizing Stainer Functions
3.1 System Setup Menu 30
Accessing the System Setup Menu 30
Stain Programs 30
Creating a Stain Program 31
Editing, Renaming, or Adjusting Stain Programs 32
Administrator and User Accounts 33
Using Reagent Information Tracking 35
Modifying Level Detect Functions 36
Changing User Language 36
Setting the Date and Time 37
System Log 37
Controlling Beeper Alerts 38
QC/Maintenance Tracking 38
Restoring Software Defaults 40
3.2 Recording Specimen and Reagent Information 41
Scanning Slides with the Barcode Reader 41
Scanning Reagent Bottles with the Barcode Reader 41
Manually Entering Specimen Information 43
Manually Entering Reagent Information 43
3.3 The Help Menu 44
Using Help 45
Section 4: Operating the Stainer
4.1 Operating Instructions 46
Suggested Staining Protocol 46
Performing a Hub Pattern Test 46
Figure 7: Correct Hub Pattern Test Result 46

Figure 8: Incorrect Hub Pattern Test Result 46
Loading the Carousel 47
Figure 9: Loading a 12-Slide Carousel (Slide Labels Toward Outer Rim) 48
Figure 10: Loading a 30-Slide Carousel (Slide Label Toward Center) 48
Figure 11: Reattaching the Carousel Lid 48
Using Blocking Slides 48
Figure 12: Using Blocking Slides 48
Performing a Stain Cycle 49
Unloading the Carousel 49
Monitoring Reagent and Waste Levels 50
Replacing a Reagent Bottle 51
Adding Reagent F to the External Bottle 51
Emptying the Waste Container 52
Section 5: Preventative Maintenance and Safety
5.1 Preventive Maintenance 53
Daily Maintenance/Quality Control (QC) 53
Weekly Maintenance 53
Monthly Maintenance 54
Annual Maintenance 54
Using the Preventive Maintenance Log 54
5.2 Storing the Instrument 56
Preparing for Long-Term Storage 56
5.3 Replacing Fuses 57
5.4 Cleaning the Stainer and Carousels 58
Cleaning Liquid Spills 58
Cleaning Broken Slides 58
Cleaning External Bottle 58
5.5 Decontaminating the Stainer and Carousels 59
Solutions for Decontaminating the Instrument 59
Figure 13: Lid Latch and Locking Pin Hole Locations 59
Decontaminating the Instrument 59
5.6 Shipping or Disposing of the Stainer or Carousels 61
Shipping the Instrument or Carousels 61
Disposing of the Instrument or Carousels 61
Section 6: Nozzle Maintenance and Performance
6.1 Nozzle Disassembly and Cleaning 62
6.2 Nozzle Reassembly 63
6.3 Manual Priming 64
6.4 Performing the Volume Test 65
6.5 Line Flush 67
6.6 Performing the Slide Pattern Test 68
Figure 14: Correct Slide Pattern Test Result 68
Figure 15: Incorrect Slide Pattern Test Result 68
Section 7: Solving Problems
7.1 Troubleshooting 69
7.2 Instrument Malfunction 75
Air or Reagent Leaks 75
Reagent Level Detect System Errors 76
Calibrating the Reagent Level Detect System 76
Calibrating the Touchscreen 77
7.3 Service Information 78
Section 8: Cytopro®Cytocentrifuge
8.1 Cytopro Cytocentrifuge Information 79
Functional Description 79
Key Features 79
Intended Use 79

Appendix A: Reagents (Stains, Washes, Agents) 80
Aerospray® Cytology Reagents 80
Critical Reagent Components 81
Appendix B: Risk and Safety Phrases 82
Appendix C: Accessories and Supplies 87
Appendix D: Stain Modes and Programming Options 88
General Settings 89
Intensity Stain Settings 90
Wash Settings 91
Appendix E: Technical Background of Papanicolaou Staining 97
To Remove Carbowax 97
Introduction 98
The ELITechGroup Staining System 98
Stain Chemistry 99

7
1.1 Instrument Overview
Using This Manual
This manual provides instructions to install, operate, and maintain the Aerospray Cytology Slide Stainer/
Cytocentrifuge Model 7522. The manual is an important part of the product. Read it carefully and
completely before setup and rst use of the instrument.
If additional accident prevention and environmental protection requirements exist in the country of
operation, this manual must be supplemented by appropriate instructions to ensure compliance.
Safety Regulations
This instrument has been designed and tested in accordance with safety regulations for electrical control,
regulating, and laboratory instruments. In order to maintain this condition and ensure safe operation, the
operator must observe all the instructions and warnings contained in this manual. For current information
about applicable standards, please refer to the CE Declaration of Conformity included with the documents
shipped with this instrument.
Understanding Warnings
This manual uses three warning levels to alert the user to important information as shown in the following
examples.
WARNING!
A Warning alerts to the possibility of personal injury, death, or other serious adverse reactions
stemming from the use or misuse of this instrument or its components.
CAUTION:
A Caution alerts to possible problems with the instrument associated with its use or misuse. Such
problems include instrument malfunction, failure, damage, damage to the sample, or damage
to other property. Where applicable, a Caution may include precautions to be taken to avoid the
hazard.
NOTE: A Note reinforces or supplies additional information about a topic.
Specic Warnings
Pay particular attention to the following safety precautions. If these safety precautions are ignored, injury or
damage to the instrument may occur. Each individual precaution is important.
WARNING!
Install the Cytology stainer in a well-ventilated area. If ventilation is inadequate, operate the
instrument under a safety hood.
WARNING!
Reagents used in the stainer contain moderately hazardous chemicals that require care in
handling. Always use appropriate safety measures including gloves and eye protection when
handling reagents.
WARNING!
Always wear protective clothing and eye protection when using SS-029 nozzle cleaning solution.
Dispose of used solution properly.
Section 1: Introduction

88
Section 1: Introduction
WARNING!
If power is lost while the stainer is running, the lid will remain locked until power is restored. Do not
attempt to open the lid while power is o󰀨.
WARNING!
Electrical shock hazard: Do not open this instrument or attempt internal repairs. Refer servicing to
qualied service personnel. Contact ELITechGroup Biomedical Systems service.
CAUTION:
This instrument has been designed and tested to CISPR 11 Class A and FCC Part 15 Class A. In
a domestic environment it may cause radio interference, in which case, measures to mitigate
the interference may be necessary. This instrument complies with the emission and immunity
requirements described in the IEC 61326 series.
In an electromagnetic environment, the environment should be evaluated prior to operation of the
device.
Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g.
unshielded intentional RF sources), as these may interfere with the proper operation.
CAUTION:
To avoid serious instrument damage, always use reagents supplied by ELITechGroup. Using
reagents not supplied by ELITechGroup may void the warranty.
CAUTION:
Only spare parts supplied or specied by ELITechGroup should be used in this instrument. Using
non-approved parts may a󰀨ect the performance and safety features of the instrument. If the
instrument is used in a manner not specied by ELITechGroup, the protection provided by the
instrument may be impaired. If in doubt, contact your ELITechGroup representative.
Functional Description
The Aerospray Cytology Slide Stainer/Cytocentrifuge (Model 7522) is a dual-purpose, microprocessor-
controlled slide staining and cell preparation system. In use, atomizing spray nozzles apply fresh reagents
onto microscope slides prepared with cytological specimens. The slides are mounted in a rotating carousel
for processing.
Staining options include a Progressive and Regressive stain mode with programmable stain settings for
user customization.
Key Features
• Minimized reagent consumption
• Rapid staining
• Barcode scanner for tracking specimens and reagents
• Reagent and specimen traceability
• User traceability
• Administrator password
• Interactive touchscreen display

99
Section 1: Introduction
• Multiple languages
• High-volume staining productivity (12 or 30 slides per stain cycle)
• Automatic Clean Cycle to purge the reagent spray nozzle with approved alcohol
• Separate reservoir, delivery tube, and pump for each reagent
• Stain sequence programmability
• Reagent and waste level monitoring
• Log les
The correct accessory must be used for each function. The Cytopro® Cytocentrifuge Rotor is available as
an option o󰀨ering additional features (see Section 8).
Intended Use
The Aerospray® Cytology Slide Stainer/Cytocentrifuge Model 7522 is intended for use by laboratory
professionals to stain cytology specimens using the Papanicolaou staining method with ELITechGroup
Aerospray® Cytology Papanicolaou stains only. The optional Cytopro® rotor allows preparation of slides by
cytocentrifugation before staining.
Table 1: General Specications
Category Characteristics
Slide Carousel Capacity 1 to 12 or 1 to 30, depending on carousel
Carousel Rotation Speed Reagent application
10 rpm (±5%) for 30-slide carousel
20 rpm (±5%) for 12-slide carousel
End spin 600 to 950 rpm (±5%) max.
Cytocentrifuge Rotor Speed 100 to 2000 rpm (±5%), user programmable
Stain Consumption Refer to Approximate Stain Consumption, Table 4
Operating Time Refer to Run Time Sequence, Table 13
Display 7 in. WVGA (800 x 480 pixels) TFT
Touch Screen Controls Numeric and alpha-numeric programming keys
Drain Connection Connector on rear panel accepts male
connector attached to vinyl drain tube.
1.8 meters (6 ft.) length supplied
Ventilation Air is exhausted from the stainer via a
female ½ inch SAE pipe thread tting
Dimensions
Width
Depth
Height (lid closed)
Height (lid open)
57 cm (22 in.)
54 cm (21 in.)
25 cm (10 in.)
58 cm (23 in.)
Weight 18.0 kg (39.7 lb.)
Electrical Requirements 100 to 240 VAC (±10%) @ 50 to 60 Hz
Power Consumption 200 Watts maximum

1010
Section 1: Introduction
Category Characteristics
Fuses 2.0 Amp time-lag (Type T) (5 x 20 mm) (2
required). Manufactured by Littlefuse (Part No.
218002) or Bussman (Part No. GDC-2A)
Ambient Temperature
Operating
Storage
15 to 30 °C (59 to 86 °F)
-10 to 50 °C (14 to 122 °F)
Relative Humidity ≤ 80% non-condensing
Altitude ≤ 2000 m (≤ 6562 ft.)
Pollution Degree 2
Heat Dissipation
Maximum
Average During Staining
Average While Idle
512 Btu/hour
102 Btu/hour
41 Btu/hour
Maximum Sound Emission 72 dB
Table 2: Performance Specications
Category Characteristics
Reagent Spray Nozzles Two reagent nozzles: ABCE (which controls
these reagents under microprocessor
control) and DF (hematoxylin)
Reagents
NOTE: Use only ELITechGroup reagents. REF numbers
for this stainer include the following: SS-051A, SS-051B,
SS-051C, SS-051D, SS-051D2, SS-051E, and SS-051F.
See Appendix A for detailed information about reagents.
A – SS-051A (EA-50 Stain)
B – SS-051B (Orange G Stain)
C – SS-051C (Bluing Agent)
D – SS-051D or SS-051D2 (Hematoxylin Stain)
E – SS-051E (Alcohol Wash)
F – SS-051F (Acid Alcohol Wash)
Stain Program Settings Two example programs: Progressive 1, Regressive 1
Number of specimen slides to stain: Selectable
from main screen – 4, 6, 8, 12, 16 or Full
Stain modes: Progressive or Regressive.
Intensity settings: 0-9 within each stain mode
Staining Customization: Each setting adjustable from 0-9
Nuclear/Htox (Hematoxylin)
Orange G
Bluing
Cytoplasmic/EA
Prewash Alcohol, Prewash Bluing
Acid Alcohol Wash, Bluing Wash,
Orange G Wash, Final Wash
End Spin

1111
Section 1: Introduction
Table 3: Carousel and Rotor Information
Only the following slide staining carousels or cytocentrifuge rotor can be used in this instrument. Each
should be used following the instructions in this manual or the Cytopro Applications Manual (RP-517).
Rotor/Carousel Maximum
RPM Maximum Capacity Maximum Sample Volume
12-Slide Carousel (AC-195) 1000 rpm 12 each, 26 mm x 76 mm (1
x 3 inch) microscope slides
N/A
30-Slide Carousel (AC-196) 1000 rpm 30 each, 26 mm x 76 mm (1
x 3 inch) microscope slides
N/A
Cytopro Cytocentrifuge
Rotor (AC-160)
2000 rpm 8 each, standard
chambers, plus slides
8 each, Cytopro Magnum
chambers, plus slides
Up to 600 µL*
Up to 6 mL*
*Do not overll cytocentrifuge chambers. See Cytopro Applications Manual or Methods Manual for detailed
instructions and warnings.
Table 4: Approximate Stain Consumption (mL)
The following table provides an estimate of the volume of stain used per staining cycle when running a full
12-slide carousel when running the default settings programed on the instrument.
NOTE: The values in parentheses provide an estimate of the volume of stain used per staining cycle when
running a full 30-slide carousel.
Stain Stain Mode Cleaning Mode
Progressive 1 Regressive 1 System Clean Carousel Clean
Stain A (EA-50) 12.8 (23.4) 20.5 (38.7) 0.0 0.0
Stain B (Orange G) 11.2 (20.3) 19.0 (35.6) 0.0 0.0
Stain C (Bluing Agent) 10.6 (21.1) 16.8 (33.4) 0.0 0.0
Stain D (Hematoxylin) 12.8 (23.7) 24.0 (45.8) 0.0 0.0
Stain E (Alchohol) 30.3 (60.4) 51.4 (102.6) 3.0 0.0
Stain F (Acid Alchohol) 4.1 (5.6) 8.7 (14.7) 3.4 7.5
Table 5: Explanation of Symbols
Symbol Description
Alternating Current (AC)
Authorized Representative in the European Community
Batch Code
Biological Hazards (Biological Risks)
Catalog Number (Model Number)

1212
Section 1: Introduction
Symbol Description
Caution, Consult Accompanying Documents (Attention, see instructions for use)
CE Mark, product meets the essential requirements designated in
Annex I of the In Vitro Device Directive (IVDD) 98/79/EC.
Consult Instructions For Use
Do Not Reuse
Do not use if package is damaged
Fragile, Handle with Care
Fuse
General Symbol for Recovery, Recyclable
In vitro Diagnostic Device (In vitro Diagnostic Medical Device)
Keep away from sunlight (Keep away from heat)
Manufacturer
Waste Electrical and Electronic Equipment
Reagent stable after opening for number of months specied
I“On” (Power)
O“O󰀨” (Power)
Environment Friendly Use Period
Serial Number
Temperature Limitation – indicates high and low limits (Normal room
temperatures are specied for all ELITechGroup Reagents.)
Use By
Volume Control
General Warning, Risk of Danger
General Caution, Risk of Danger
Warning, Biological Hazard

1313
Section 1: Introduction
Symbol Description
Corrosive
Environment Hazard
Flammable
Harmful/Irritant
Oxidant
Toxic
Product to be used for manual cleaning only. Do not pump product through instrument.
Systemic health hazards

1414
Section 1: Introduction
1.2 Instrument Description
Figure 1: Front and Right-Side Panels
1. 30-Slide Carousel
2. 12-Slide Carousel
3. Lid with Safety Lock
4. Bowl
5. Front Panel with Touch Screen Display
6. Right Side Panel with Label Indicating Reagent Positions:
A. EA-50 Stain
B. Orange G Stain
C. Bluing Agent
D. Hematoxylin Stain
E. Alcohol Wash
F. Acid Alcohol Wash (bottle is o󰀨 tray)
7. Reagent Tray
1
2
A B C D E F
4
5
6
7
3

1515
Section 1: Introduction
Figure 2: Front Panel and Touchscreen
1. Standby/Ready Button
2. Touch Screen
The front panel features an interactive touchscreen display. Refer to Touchscreen and User Interface
(Section 1.3, Table 7) for more information.
Figure 3: Rear Panel
1. Level Detect Connection for Reagent F (Acid Alcohol Wash)
2. USB Ports
3. Network Ethernet Connection
4. Exhaust Vent
5. Rear Panel Label
6. Biohazard Warning Label
7. Waste Tube Connection
8. Level Detection Connection for Waste Container
9. Power Switch
10.Fuse Door
11. Power Cord Connection
12.Model/Serial Number Label
1
2
6
5
3 42
2
7891011
12
1

1616
Section 1: Introduction
Figure 4: Stainer Bowl Components
1. Nozzle DF (Reagents D Hematoxylin Stain, F Acid Alcohol Wash)
2. Nozzle ABCE (Reagents A EA-50 Stain, B Orange G Stain, C Bluing Agent, E Alcohol Wash)
3. Drive Hub
Figure 5: Nozzle Diagram
1. Mixing Insert
2. Compression Screw
3. Swirl Cone
4. Nozzle Housing
Table 6: Preventive Maintenance Kit (Catalog Number AC-184)
Component Name Description
Manual Priming Tool Primes air-locked pumps.
Silicon Grease Lubricates the nozzle threads for easy assembly.
Nozzle Wire Cleans nozzle housing orices.
12
3
1 42 3

1717
Section 1: Introduction
Component Name Description
Nozzle Cleaning Strainer Strains the nozzle parts to prevent
them from going down a drain.
Nozzle Tool Unscrews nozzles from the stainer bowl.
Nozzle Wrench Disassembles the nozzle.
Nozzle Brush Cleans nozzles without removing
them from the stainer.
Volume Test Collection
Tubes (small tube)
Collects reagents while performing the Volume Test.
Nozzle Maintenance
Tube Stand
Holds Nozzle Cleaning Tubes (large tube)
and Volume Test Tubes (small tube).
Nozzle Cleaning Tubes
(large tube)
For soaking nozzles in the Nozzle Cleaning Solution.

1818
Section 1: Introduction
Barcode Reader
An optional barcode reader is available for the Cytology Stainer/Cytocentrifuge (Model 7522).
Figure 6: Barcode Reader
Other Needed Items
The following parts are not available worldwide from ELITechGroup, but they can be obtained locally:
• Approved alcohol (Ethanol or Isopropanol)
Empty bottles are available from ELITechGroup.
CAUTION:
ELITechGroup does not provide approved alcohols. They should be purchased locally, observing
the recommendations for safety and chemical risk on the Safety Data Sheet (SDS).

1919
Section 1: Introduction
1.3 Touchscreen and User Interface
Users control all instrument functions from the interactive touchscreen display.
Table 7: Front Panel/Main Screen Function Keys
Button Name Description
Standby/Ready With instrument power ON:
Blue = Ready
Amber = Standby
Pressing standby runs a System Clean Cycle and
places instrument into standby mode.
The Standby/Ready button also accesses the touchscreen
calibration function. Refer to System Setup Menu (Section 3.1).
Maintenance Accesses features for verifying proper nozzle performance
and places pumps in a testing sequence. Accesses the line
priming, Pattern Test, Volume Test, and Line Flush functions.
System Clean/
Carousel Clean
Performs the selected Clean Cycle. System Clean
(left button). Carousel Clean (right button).
Cyto Enters the Cytocentrifuge mode.
System Information Shows the system information, including serial number
and software version. Allows access to the System Setup
features. Refer to System Setup Menu (Section 3.1).
Help Opens the software Help le.
Programs Allows users to select or edit programs.
Start/Load Slides Starts a Stain or Cytocentrifuge cycle. Button is inactive until a
program is created. Refer to Creating a Stain Program (Section 3.1).
With Slide Tracking enabled, opens the Scan
and Load Slides menu (Section 3.2).
Number of Specimen
Slides
Selects the number of specimen slides in the carousel.
Users staining an odd number of specimen slides should
press the next higher specimen slide number icon.
Back Returns to the previous menu.
Stop Aborts any operation.
OK Indicates completion of current task.

2020
Section 1: Introduction
Button Name Description
System Setup Allows users to modify the software settings.
See System Setup Menu (Section 3.1).
Table 8: System Setup Keys
Button Name Description
Stain Programs Allows users to create, edit, and delete stain programs.
Cyto Programs Allows users to create, edit, and delete cytocentrifuge programs.
Reagents Allows users to edit reagent information.
Users Allows users to create and change user accounts.
QC/Maintenance Tracking Enables slide tracking, preventive maintenance
tracking, and reagent tracking.
Level Detect Allows users to manage the automatic
reagent level detection system.
Language Allows users to change the display language.
System Log Allows users to control logging functions.
Network Settings Allows users to change network settings.
Beeper Allows users to change audible alerts.
Set Date/Time Allows users to set the date and time.
Restore Defaults Restores programming to default settings.
Login Enters Login sequence for authorized users.
Logout Logs authorized users out. Users must
log in again to use the stainer.
Save Saves the entered or selected information.
Add Enters programming mode for creating staining and cytocentrifuge
programs. Also allows the system administrator to authorize new
users. Allows manual entry of slide or specimen information.