Emulate Zoe-CM2 User manual

Zoë-CM2TM
User Guide

Page 3
Zoë means life. The Zoë-CM2 Culture Module is designed to sustain the life of cells within
Organ-Chips.
The instrument automates the precise conditions needed for simultaneous cell culture of up
to 12 Organ-Chips. It supplies the dynamic ow of media and recreates the mechanical forces
of breathing motions or peristalsis that help Organ-Chips recreate the microenvironment cells
experience in the body.
Zoë-CM2 gives users the ability to independently control the ow rate of media through
both the top and bottom channels of Organ-Chips. It also allows users to determine stretch
parameters—including frequency and amplitude—of the Organ-Chip membrane. What's more,
the Zoë-CM2 Culture Module has automated algorithms to prime the uidic channels of Organ-
Chips with media and remove any bubbles from the uidic channels. Zoë-CM2 can be operated
with controls located on the instrument or remotely monitored and controlled with the Zoë
Manager web application.
This user guide has essential instructions for how to safely and eectively operate Zoë-CM2.
Please ensure all users thoroughly read and understand this guide before operation.
This product is for research use only.
Zoë®Culture Module

InstallationIntroduction Specications Safety
Page 7 Page 17 Page 21
Zoë-CM2 Culture Module / Table of Contents
Introduction
Page 13
1 2 3 4
Zoë Culture Module
Table of Contents

Page 5
5
Use
Page 45 Page 49 Page 53
Use
CareSettings Help &
Support
Page 55
6 8 9
Page 29
Updating Firmware &
Downloading Logs
7

Page 7
Introduction
Page 7
1
This section provides an overview of Zoë
and its functions.
Equipment (what’s in the box)
1. Zoë-CM2 Culture Module
2. Chip Cradles
3. Gas & Vacuum Supply Line
4. Zoë Core Update Cable
5. SensorPush HT1 Temperature and Humidity Smart Sensor
6. Metal Spudger Pry Tool
7. Ethernet Cable
8. 24V Power Supply

Zoë-CM2 Culture Module / Introduction
Zoë-CM2 Culture Module
Components
1. Zoë-CM2 Culture Module
2. Power Button
3. Dial
4. Dial Button
5. Display
6. Activation Button
7. Bay
8. Pod™Assembly
9. Tray
Power Button – Used to
power the device on and o.
Dial – Used for selecting
elements on the display, basic
navigation, and adjusting
values.
Dial Button – Used to conrm
selections, parameters, and
other edits to system settings.
Display – Shows the current
Zoë parameters, displays
system status, and provides
alerts and messaging to the
user.
Bays – Zoë has a left and
right Bay. Each Bay holds up
to six Pod®Portable Modules,
or 12 total.
Bay Activation Buttons –
Each of the two Bay Activation
Buttons can be pressed to
raise or lower the manifolds to
engage the Pods and resume
or pause ow independently
in each Bay.

Page 9
1. Bottom Channel Inlet Port
2. Top Channel Inlet Port
3. Vacuum Ports
4. Top Channel Indicator
5. Top Channel Outlet Port
6. Bottom Channel Outlet Port
7. Chip Body
8. Top Channel Indicator
Organ-Chip
Conguration

1. Pod Reservoir Lid
2. Pod Reservoir
3. Organ-Chip
4. Chip Carrier
Pod
Configuration
Zoë-CM2 Culture Module / Introduction

Page 11
1. Top Channel In
2. Top Channel Out
3. Vacuum Chamber
4. Bottom Channel Out
5. Bottom Channel In
Pod
Components

Page 13
Specications
Page 13
2
This section provides specic technical information and
operational requirements. Included are system dimensions,
thermal and gas requirements, and modes of operation.

Zoë-CM2 Culture Module / Specications
8.6 in
16.9 in
7.0 in
Manufacturer Emulate, Inc.
27 Drydock Avenue
Boston, MA 02210
Assistance Contact
[e-mail]
Use The Zoë-CM2 Culture Module is
for research use only
Zoë-CM2
Specications
General Model: CM2
Weight: 10.9kg (24 lbs.)
Power Consumption: 2.16 kW/day
Capacity: 12 Pods
Operating Instrument Rating: 24 VDC, 3.5 A, 50-60 Hz
Requirements Gas Input Pressure: 276 kPa +/- 35 (40 psig +/- 5)
Gas Input Composition: 5% CO2, balance air
Vacuum Input Pressure: -70 kPa (-10.2 psig)
Environmental Operating Temperature: 20–38ºC (68-100. 4ºF)
Relative Humidity: 0–90%
Max Altitude: 2,000 meters (6,562 feet)
Storage Temperature: -5–40ºC (23–104ºF)
Storage Humidity: 30-85%, non-condensing
Technical Flow Rate Ranges: 0 μL / h or 10–1,000 μL / h
Stretch: 0–12%
Stretch Frequency: Min: 0.01 Hz Max: 0.4 Hz

Page 15
Zoë-CM2 has two dierent modes of operation.
It can operate:
1) Oine (not connected to the
internet)
2) Connected to the Emulate Cloud
and operated via Zoë Manager
(recommended)
The table to the right outlines the basic
functionality and benets of each mode.
We take data privacy seriously. For more
information, please reference our Data Privacy
& Security Policy for Emulate Software
Applications.
Mode of Operation
Oine
Cloud-Connected
via Ethernet
Description
(Basic Functionality)
Zoë-CM2 is not connected
to the internet.
Zoë-CM2 can only be
operated using the screen
on the instrument.
Zoë-CM2 has access
to the internet.
Zoë-CM2 can be operated
by using either the screen
on the instrument or the
web.
Advantages
No internet connection needed.
Remotely monitor Zoë status
and parameters as well as
pause and ow functions.
Run commands on Zoë, such as
Flush and Regulate, remotely.
When Emulate is given
permission, Emulate eld
engineers can pull diagnostic
data from an instrument for
streamlined support.
When a software update is
available, Zoë can be updated
over the air.
Zoë-CM2
Operation

Page 17
Safety
Page 17
3
This section includes recommended precautions when
handling and using Zoë-CM2. The information here describes
how users can minimize the chance of harming themselves or
the module during operation.

Zoë-CM2 Culture Module / Safety
The Instructions Symbol
The product is marked with this warning symbol where it is necessary for the user to
refer to the instructions in the user guides.
Regulatory Compliance and Testing
This product has been tested to the requirements of:
61010-1 (IEC, EN, UL, CSA)
IEC 61010-2-10 Safety Requirements for Electrical Equipment for measurement,
control, and laboratory use
EMC Testing to EN61326-1:2013, FCC Part 15 Subpart B:2021, ICES-003 Issue 6, EN
55032:2015/AC:2016
Zoë-CM2
Safety

Page 19
Zoë-CM2 is not sterile out of the box. Clean thoroughly with 70%
ethanol before placing in the incubator to decrease the risk of
contamination.
Never attempt to maintain or sanitize Zoë without first disconnecting
from the power source.
Vent and disconnect all Gas Supply Lines before moving, sanitizing,
or maintaining Zoë.
Zoë is heavy, so exercise care when moving it. Incubator shelving
may not support Zoë when it is partially pulled out of the incubator.
Make sure the instrument is placed on a secure and sturdy surface.
Never insert fingers or foreign objects into the Bays. Injury or
instrument damage may result.
Always follow in-house safety protocols before handling compressed
gas.
Users are responsible for the proper disposal of any single-use
components that encounter biological material.
Service and maintenance should only be completed by Emulate-
certified personnel. Never attempt to disassemble or repair Zoë
without assistance.
Please be considerate of the instrument temperature range. Do not
attempt to operate Zoë-CM2 in a temperature range outside of the
one presented in this manual.
To power Zoë-CM2, only use the 24V Power Supply and Power Cord
provided with the instrument. Note that this Power Cord is dierent
from those of Orb and Zoë-CM1. Do not plug in the Power Supply
provided with the Orb or Zoë-CM1 into Zoë-CM2.
If two Zoës are being placed in an incubator, arrange them on the
bottom shelf alongside each other to ensure a balanced temperature
in the incubator.
It is essential that all instructions are followed, and all warnings are understood. Failure to adhere to the instructions or
operate the equipment within the stated specications may cause injury, damage to the instrument, or issues with chip
performance.
Zoë-CM2
Caution Statements