EUTECH INSTRUMENTS DO 6 - REV 2 User manual

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Instruction Manual
DO 6
Economy Hand-held Dissolved Oxygen Meter
68X243622
Rev 2 Apr 08
Part of Thermo Fisher Scientific

Preface
This manual serves to explain the use of the DO 6 Dissolved Oxygen Handheld.
This manual functions in two ways: first as a step by step guide to help you
operate the meter; second, as a handy reference guide.
This manual is written to cover as many anticipated applications of the DO6
palm-top meter as possible. If there are doubts in the use of the Dissolved
Oxygen Handheld, please do not hesitate to contact the nearest Eutech
Instruments / Oakton Instruments authorized Distributor.
Eutech Instruments/Oakton Instruments will not accept any responsibility for
damage or malfunction to the meter caused by improper use of the instrument.
The information presented in this manual is subject to change without notice as
improvements are made, and does not represent a commitment on the part of
Eutech Instruments / Oakton Instruments.
Copyright © 2004
Eutech Instruments Pte Ltd
Oakton Instruments
All rights reserved.

TABLE OF CONTENTS
1INTRODUCTION ..........................................................................1
2DISPLAY & KEYPAD FUNCTIONS.............................................2
2.1 DISPLAY .................................................................................2
2.2 KEYPAD ..................................................................................3
3PREPARATION............................................................................4
3.1 INSERTING &REMOVING RUBBER BOOT ................................4
3.2 INSERTING THE BATTERIES .....................................................5
3.3 BATTERY REPLACEMENT........................................................6
3.4 DISSOLVED OXYGEN PROBE INFORMATION............................7
3.5 CONNECTING THE PROBE TO METER ......................................9
3.6 SWITCHING THE METER ON..................................................10
3.7 MEASUREMENT MODE SELECTION:- PERCENTAGE
SATURATION (%), CONCENTRATION (MG/L)(PPM)AND
TEMPERATURE(T).................................................................11
4CALIBRATION...........................................................................12
4.1 IMPORTANT INFORMATION ON METER CALIBRATION...........12
4.2 PREPARING THE METER FOR CALIBRATION ..........................13
4.3 TEMPERATURE CALIBRATION ...............................................15
4.4 DISSOLVED OXYGEN CALIBRATION IN %SATURATION........17
4.4.1 To calibrate 100% Saturation:................................................17
4.4.2 To calibrate 0% Saturation.....................................................19
4.5 DISSOLVED OXYGEN CALIBRATION IN MG/L OR PPM
CONCENTRATION MODE ........................................................20
4.5.1 To calibrate in mg/L (ppm) Concentration mode:...................20
5MEASUREMENT........................................................................22
5.1 WITH AUTOMATIC TEMPERATURE COMPENSATION (ATC)..22
5.2 MANUAL TEMPERATURE COMPENSATION ............................23
5.3 TAKING MEASUREMENTS .....................................................25
5.3.1 To take measurements: .........................................................25

5.4 TAKING PRESSURE/SALINITY COMPENSATED DO
MEASUREMENTS...................................................................26
5.4.1 Pressure Setting Adjustment..................................................26
5.4.2 Salinity Setting Adjustment ....................................................29
6HOLD FUNCTION......................................................................30
7ADVANCED SETUP FUNCTIONS.............................................31
7.1 ADVANCED SETUP OVERVIEW..............................................31
7.1.1 To enter the % Saturation or Temperature Set Up menu: .....31
7.1.2 To enter the mg/L (ppm) Concentration Set Up menu:..........31
7.2 (COF.1) CONFIGURATION MENU..........................................36
7.2.1 Selection of Automatic Temperature Compensation..............36
7.2.2 Selection of mg/L or ppm mode .............................................38
7.3 (CAL.2) CALIBRATION DATA...............................................39
7.3.1 Viewing the % Saturation calibration data..............................39
7.3.2 Viewing the mg/L (ppm) Concentration calibration data ........39
7.4 (ELE.3) ELECTRODE PROPERTIES.........................................40
7.4.1 Viewing the electrode Slope Factor .......................................40
7.4.2 Viewing the % Saturation Offset (Only available in %
Saturation or Temperature Set Up menu):...............................................40
7.4.3 Viewing the 100% Saturation mV value.................................41
7.4.4 Viewing the 0% Saturation mV value.....................................41
7.5 (ATO.4) AUTOMATIC OFF ....................................................43
7.6 (RST.5) RESET TO FACTORY DEFAULT..................................44
7.6.1 Calibration Reset....................................................................44
7.6.2 User Reset .............................................................................46
7.7 (OFS.6) %SATURATION OFFSET ADJUSTMENT....................47
7.8 (DPR.7) DISSOLVED OXYGEN PARAMETERS.........................49
7.8.1 Pressure Setting Adjustment..................................................49
7.8.2 Salinity Setting Adjustment ....................................................51
8PROBE CARE AND MAINTENANCE........................................52
8.1 PROBE CARE .........................................................................53
8.2 PRE-MEMBRANED CAPS REPLACEMENT...............................53
8.3 TO REPLACE THE PROBE PRE-MEMBRANED CAP ....................54

8.4 ELECTROLYTE SOLUTION .....................................................55
9TROUBLE-SHOOTING GUIDE..................................................56
10 ERROR MESSAGES..................................................................57
11 FACTORY DEFAULT SETTINGS..............................................58
12 SPECIFICATIONS......................................................................60
13 ACCESSORIES..........................................................................62
14 ADDITIONAL INFORMATION....................................................63
14.1 DISSOLVED OXYGEN ............................................................63
14.1.1 General Information ...............................................................63
14.1.2 Measurement Units................................................................65
14.1.3 What Is Being Measured?......................................................65
14.1.4 Air Calibration.........................................................................66
14.1.5 Applications............................................................................66
15 WARRANTY...............................................................................68
16 RETURN OF ITEMS...................................................................69

Instruction Manual DO 6
1
1 INTRODUCTION
Thank you for purchasing the DO 6 Dissolved Oxygen Handheld. This economy
microprocessor-based handheld has a large custom LCD (Liquid Crystal
Display) for clear and easy reading.
The DO 6 offers the measurement of dissolved oxygen in the percentage
saturation mode and in the concentration mode. Temperature measurement is
also available in degrees Celsius. The meter ensures accurate measurement of
the Dissolved Oxygen values through its temperature, barometric pressure and
salinity compensation features.
Your meter includes a dissolved oxygen probe, refill solution, a rubber boot, 4
alkaline “AAA” batteries, and instruction manual.
Please read this manual thoroughly before operating your meter.
To order other accessories and standard solutions, please refer to the Section
on Accessories for more information.

Instruction Manual DO 6
2
2 DISPLAY & KEYPAD FUNCTIONS
2.1 Display
The meter has a large custom LCD that consists of 14-segment 4 digit display
and operation annunciators for Percentage Saturation measurement mode (%),
Concentration measurement mode (mg/L or ppm) and Temperature
measurement mode in degrees Celsius (T). Other annunciators include “A”
(when the ATC function is activated), “CAL” (when meter is in calibration mode)
and battery diagram for low battery condition. See Figure 1.
milligram per litre
(Concentration Mode)
mg/l
ppm
%
TA
14-segment Liquid Crystal Display
Low Battery annunciato
r
Calibration Mode
Annunciator
CAL
Temperature Mode
A T C annunciator
parts per million
Percentage
(Percentage Saturation
Mode)
Figure 1: Active LCD display for DO 6

Instruction Manual DO 6
3
2.2 Keypad
The DO 6 meter has 6 keys on its splash-proof keypad; ON/OFF,
HOLD/ENTER CAL, MODE, ▲and ▼keys. Some buttons have several
functions depending on its mode of operation.
ON
OFF
ON/OFF – Powers on and shuts off the meter. The meter will start in the
measurement mode it was in when last switched off.
CAL
CAL – Activates the calibration mode for Percentage Saturation,
Concentration and Temperature calibrations.
Deactivates calibration or setup without confirming a value in calibration
mode.
HOLD
ENTER
HOLD -Activates/Deactivates freezing of the measured reading while in
measurement mode.
ENTER - Confirms the calibration values in Calibration mode and the
selections in the SETUP menu.
▲▼ – Sets the calibration values during the Concentration and
Temperature calibration.
Scrolls through each SETUP and its sub group menu.
Set offset adjustments and configuration settings.
MODE
MODE - Selects the measurement option between DO Percentage
Saturation measurement; DO Concentration measurement and
Temperature measurement.
When pressed together with ON/OFF key during power on, SETUP
mode is selected. This menu allows meter customization with
preferences such as activating ATC selection, DO concentration
measurement unit selection, viewing of the last calibration data,
viewing of the electrode properties, selecting the auto power off,
resetting calibration data or meter settings back to factory default,
setting the offset adjustments, setting barometric pressure in Hg or
PA for barometric pressure compensation and setting of the salinity
value for salinity compensation.

Instruction Manual DO 6
4
3 PREPARATION
3.1 Inserting & Removing Rubber Boot
1) To remove meter from rubber boot, push out from the bottom edges of
meter until it is completely out of boot. Ensure that the cables of Dissolved
Oxygen electrode or temperature probe are not connected.
2) To insert meter into rubber boot, slide in from the top of meter before
pushing the bottom edges of meter down to set it into position. Lift up the
stand at the back of meter for bench top applications if necessary.
Figure 1: Inserting or removing the rubber boot

Instruction Manual DO 6
5
3.2 Inserting the Batteries
The battery compartment is found at the back of instrument as shown in Figure
3. To open the battery compartment first remove protective rubber boot/stand
then:
1) Push in the direction of arrow and lift up the cover.
2) Note the polarity of battery before inserting into position.
3) After replacement, place cover back and press down until it locks tight.
Figure 2: Inserting the batteries

Instruction Manual DO 6
6
3.3 Battery Replacement
A low battery annunciator in the LCD alerts you when battery power is running
low. See Figure 4. Replace with the same type as recommended by the
manufacturer.
%
Figure 3: Low Battery Condition
Caution: Power off the meter when changing battery.

Instruction Manual DO 6
7
3.4 Dissolved Oxygen Probe Information
The DO 6 is supplied with a Dissolved Oxygen
Probe that works on galvanic principle, that is, it
does not require any polarising voltage from
your meter. Rather it will generate a millivolt
signal proportional to the amount of oxygen in
the solution.
Your DO Probe has a twin cable, one with a
BNC connector for the DO measurement input,
and the other with a phono jack plug for the
temperature measurement input. Its sensing
area consists of a cathode, anode, and an inner
electrolyte which is separated from your sample
solution by an oxygen permeable membrane
pre-membraned to the probe’s detachable cap.
Re conditioning of your probe is made easy with
the introduction of this specially designed
detachable pre-membraned cap.
This light weight probe (93X233912 /35642-50)
has a built-in temperature sensor for Automatic
Temperature Compensation (ATC) with 12mm
diameter epoxy body housing and 16 mm
diameter Noryl detachable cap. Its compact
sensing area reduces air entrapment which
makes it easy to obtain fast, accurate and
stable readings. Simply stir the probe in the
solution being measured. Shaking will aid to
remove bubbles if needed before taking a
reading. Also, always ensure to remove water
drops from the membrane when calibrating in
air.
Proper use of probe is essential to ensure that
the optimum measurement is taken in a short
Figure 4: 12 mm Galvanic DO
probe (93X233912 / 35642-50)
Detachable
Pre-membraned Cap
(Probe Sensing Area)
16 mm
12 mm

Instruction Manual DO 6
8
time. Always immerse the probe beyond the pre-membraned cap. The minimum
water flow rate is 2 inch/second across membrane. The temperature range for
use is within 0 to 50 °C.
Always ensure that the probe’s membrane is protected against any scratches or
dents. The whole pre-membraned cap has to be replaced if the membrane is
damaged. It is also important to keep the membrane clean so as to produce an
optimum and accurate measurement.
See Section 8 - “Probe Care and Maintenance” for more information.

Instruction Manual DO 6
9
3.5 Connecting the Probe to Meter
1) Insert the BNC connector from the electrode to the BNC connector socket
on the meter accordingly and rotate connector clockwise until it locks. See
Figure below.
2) To remove, simply rotate the connector in counter-clockwise direction until
it unlocks, and slide the connector off the socket.
3) Plug the phono jack of temperature sensor into the phono socket of the
meter as shown in Figure below.
Caution: Do not pull or force the probe cord or the probe wires might
disconnect.
Note: Keep connectors clean. Do not touch connector with soiled hands.
BNC Connector for
DO probe
Phono jack for
Temperature probe
Figure 5: DO 6 probe to meter connections

Instruction Manual DO 6
10
3.6 Switching the Meter On
When switching the meter on, it will go
through a series of display, eventually
showing the revision number of the meter
before going to the measurement mode.
The mode of measurement which the
meter displays will be similar to the last
measurement mode to which the meter is
switched off.
Press ON/OFF key to power up your
meter.
1) All LCD segments will light up for 1.5
seconds.
2) The display will then momentarily
show the meter’s identification “DO 6”
with the annunciators for percentage
saturation mode and concentration
mode.
3) The display continues to switch to
display meter’s revision number for
1.5 seconds before finally showing
the measurement mode.
4) Either percentage saturation
measurement mode, concentration
measurement mode or temperature
measurement mode will be displayed
following the previous measurement
mode to which the meter is switched
off.
CAL 13 CAL 24
mg/l
NTU
ppm
%pH
TAvg
mg/l
ppm
%
ON
OFF
A
%
Figure 6: Power Up sequence

Instruction Manual DO 6
11
3.7 Measurement Mode Selection:- Percentage
Saturation (%), Concentration (mg/L)(ppm) and
Temperature(T)
By simply pressing the MODE key, you can select the measurement mode of
percentage saturation measurement, concentration measurement or
temperature measurement.
The customized annunciator shall indicate the selection of the measurement
mode.
mg/l
A
TA
A
%
MODE
MODE
MODE
Percentage Saturation
Concentration Mode
Temperature Mode
Automatic Temperature
Compensation
Figure 7: Measurement mode selection using the mode key

Instruction Manual DO 6
12
4 CALIBRATION
4.1 Important Information on Meter Calibration
Your meter has three measurement modes namely the DO % saturation
measurement mode, the DO mg/L (ppm) concentration mode and the
Temperature measurement mode.
Since the mg/L (ppm) concentration value of dissolved oxygen varies with
temperature, barometric pressure and salinity, the meter with its electrode has
to be calibrated with consideration to these factors to ensure a proper
measurement. Therefore, it is very important to set the proper temperature,
barometric pressure and salinity values in the meter prior to any calibration or
measurement process. Since the dissolved oxygen measurements in %
saturation will linearly affect the mg/L (ppm) concentration measurement, it is
important that the meter has to be first calibrated in the % Saturation mode and
temperature mode before commencing the mg/L (ppm) concentration mode
calibration.
In % saturation calibration mode, the dissolved oxygen calibration values will
vary according to the barometric pressure correction set in the meter. Therefore
it is necessary to set the barometric pressure first before proceeding to %
Saturation calibration. The following table is an example which explains the
acceptance calibration values when % Saturation mode calibration is attempted
under different absolute value and under two different barometric pressures.
% Saturation factory
default value
Acceptance Calibration Value
(760mmHg)
Acceptance Calibration Value
(700mmHg)
less than 10% 0% 0%
10.1% to 49.9% Err.1 (error 1) Err.1 (error 1)
50% to 200% 100% 92.1%