Fisher Scientific Isotemp 178L 120V User manual

i-ii Peltier Low Temp. Incubators Fisher Scientific
© 2016 Thermo Fisher Scientific. All rights reserved.
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the English version of these operating instructions is binding.
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Fisher Scientific Peltier Low Temp. Incubators i
C
Chapter 1 Safety Notes.....................................................................................................................1-1
Basic Operating Precautions ............................................................................................................... 1-1
Operational Safety Rules .................................................................................................................... 1-2
Warranty ............................................................................................................................................ 1-2
Explanation of Safety Information and Symbols................................................................................. 1-3
Safety Notes and Symbols Used Throughout These Operating Instructions ................................... 1-3
Additional Symbols for Safety Information..................................................................................... 1-4
Symbols on the low temperature incubator..................................................................................... 1-5
Intended Purpose of the low temperature incubator ........................................................................... 1-6
Intended Purpose of the low temperature incubator ....................................................................... 1-6
Incorrect Use .................................................................................................................................. 1-6
Standards and Directives .................................................................................................................... 1-6
Chapter 2 Delivery of the low temperature incubator.................................................................... 2-1
Packaging ........................................................................................................................................... 2-1
Acceptance Inspection ........................................................................................................................ 2-1
Scope of Supply.................................................................................................................................. 2-2
Chapter 3 Installation........................................................................................................................3-1
Ambient Conditions........................................................................................................................... 3-1
Location Requirements................................................................................................................... 3-1
Intermediate Storage........................................................................................................................... 3-2
Room Ventilation............................................................................................................................... 3-2
Space Requirement......................................................................................................................... 3-3
Table-top low temperature incubators ............................................................................................3-3
Floor Stand low temperature incubators ......................................................................................... 3-4
Transport ........................................................................................................................................... 3-5
Stacking kit ........................................................................................................................................ 3-7
Installing the Stacking Feet................................................................................................................. 3-7
Installing the Stacking Adapter........................................................................................................... 3-8
Installing the Anti-tilt Anchor ............................................................................................................ 3-9
Chapter 4 Product Description ........................................................................................................4-1
Fisher Scientific low temperature incubator overview ......................................................................... 4-1
Peltier System ..................................................................................................................................... 4-6
Safety Devices..................................................................................................................................... 4-7
Work Space Atmosphere .................................................................................................................... 4-7
Door Switch ....................................................................................................................................... 4-7
Sensing and Control System............................................................................................................... 4-7
Data Communications & Alarm Interface.......................................................................................... 4-9
RS-232 Interface............................................................................................................................. 4-9
Contents

Contents
ii Peltier Low Temp. Incubators Fisher Scientific
AC Power Socket............................................................................................................................ 4-9
Fuses............................................................................................................................................. 4-10
Work Space Components................................................................................................................. 4-11
Inner Chamber............................................................................................................................. 4-11
Access Port ................................................................................................................................... 4-11
Shelf System ................................................................................................................................. 4-12
Additional Tube Access Ports ........................................................................................................... 4-13
Chapter 5 Installation procedures...................................................................................................5-1
Table-top Peltier low temperature incubators ..................................................................................... 5-1
Installing the Shelf System.................................................................................................................. 5-1
Initial Installation ........................................................................................................................... 5-1
Installing the Perforated Shelves ......................................................................................................... 5-2
Preparing the useable space................................................................................................................. 5-2
Installation or Removal of the Support Rails................................................................................... 5-3
Installing and Uninstalling the Air Baffle........................................................................................ 5-4
Levelling the low temperature incubator.........................................................................................5-4
Commissioning of floor stand low temperature incubators ................................................................. 5-5
Installing/Removing air baffles (381L Series).................................................................................. 5-5
Commissioning, general ..................................................................................................................... 5-6
Installing the Shelf Support Brackets .............................................................................................. 5-6
Installing the Perforated Shelves ..................................................................................................... 5-7
Mains connection............................................................................................................................... 5-7
Connecting to the Power Supply Source......................................................................................... 5-7
Connecting the RS-232 Interface ....................................................................................................... 5-8
Interconnecting the low temperature incubator with a Computer................................................... 5-9
Wiring the Alarm Contact................................................................................................................ 5-11
Functional Description................................................................................................................. 5-11
Alarm Relay Specifications............................................................................................................ 5-11
Connection Example .................................................................................................................... 5-12
Chapter 6 Operation..........................................................................................................................6-1
Preparing the Peltier low temperature incubator.................................................................................6-1
Starting Operation.............................................................................................................................. 6-1
Chapter 7 Handling and Control......................................................................................................7-1
Powering Up ...................................................................................................................................... 7-5
Switching the low temperature incubator Off / Powering Down ........................................................ 7-5
Temperature Set Value ....................................................................................................................... 7-6
Timer ................................................................................................................................................. 7-7
Stopping a Timer............................................................................................................................ 7-8
Settings............................................................................................................................................... 7-9
Error Log........................................................................................................................................ 7-9
Calibration ................................................................................................................................... 7-10
Temperature Display Unit............................................................................................................ 7-11
Chapter 8 Shut-down........................................................................................................................8-1
Shutting the Peltier low temperature incubator Down........................................................................ 8-1

Contents
Fisher Scientific Peltier Low Temp. Incubators iii
Chapter 9 Cleaning and Disinfection ...............................................................................................9-1
Cleaning..............................................................................................................................................9-1
Cleaning Exterior Surfaces...............................................................................................................9-1
Wipe / Spray Disinfection...................................................................................................................9-1
Preparing the Manual Wipe/Spray Disinfection ..............................................................................9-3
Pre-disinfection ...............................................................................................................................9-3
Cleaning..........................................................................................................................................9-4
Final Disinfection............................................................................................................................9-4
Chapter 10 Maintenance....................................................................................................................10-1
Inspections and Checks .....................................................................................................................10-1
Regular Checks..............................................................................................................................10-1
Semi-annual Inspection.................................................................................................................10-1
Service Intervals.................................................................................................................................10-2
3-monthly service ..........................................................................................................................10-2
Annual Service...............................................................................................................................10-2
Preparing Temperature Calibration...................................................................................................10-3
Comparison Measurement Procedure................................................................................................10-3
Temperature Calibration Procedure ..................................................................................................10-4
Replacing the Door Seal....................................................................................................................10-5
Replacing the Power Cord.................................................................................................................10-6
Returns for Repair.............................................................................................................................10-6
Chapter 11 Disposal...........................................................................................................................11-1
Overview of Materials Used ..............................................................................................................11-1
Chapter 12 Error Codes.....................................................................................................................12-1
Chapter 13 Technical Data................................................................................................................13-1
Chapter 14 Spare Parts and Accessories........................................................................................14-1
Chapter 15 Device Log ......................................................................................................................15-1
Chapter 16 Contact............................................................................................................................16-1

Contents
iv Peltier Low Temp. Incubators Fisher Scientific

Fisher Scientific Peltier Low Temp. Incubators i
L
Figure 3-1 Table-top Peltier low temperature incubators, dimensions and required clearances............................... 3-3
Figure 3-2 Floor stand Peltier low temperature incubators, dimensions and required clearances ............................ 3-4
Figure 3-3 Lift Points............................................................................................................................................. 3-5
Figure 4-1 Fisher Scientific Isotemp 178L Front View........................................................................................... 4-2
Figure 4-2 Fisher Scientific Isotemp 178L Rear View ............................................................................................ 4-3
Figure 4-3 Fisher Scientific Isotemp 381L Front View........................................................................................... 4-4
Figure 4-4 Fisher Scientific Isotemp 381L Rear View ............................................................................................ 4-5
Figure 4-5 IMP Peltier low temperature incubator in cooling mode ...................................................................... 4-6
Figure 4-6 IMP Peltier low temperature incubator in heating mode ...................................................................... 4-6
Figure 4-7 PT100 Sensor Isotemp 178L ................................................................................................................ 4-8
Figure 4-8 PT100 Sensor Isotemp 381L ................................................................................................................ 4-8
Figure 4-9 Signal Interfaces and Power Socket....................................................................................................... 4-9
Figure 4-10 T 2A H 250 fuse................................................................................................................................. 4-10
Figure 4-11 Access Port ......................................................................................................................................... 4-11
Figure 4-11 Shelf System ....................................................................................................................................... 4-12
Figure 5-1 Sliding the Retaining Spring into the Support Rail............................................................................... 5-1
Figure 5-2 Installing the Shelving .......................................................................................................................... 5-2
Figure 5-3 Support Rail Installation....................................................................................................................... 5-3
Figure 5-4 Removing the Air Baffle ....................................................................................................................... 5-4
Figure 5-5 Removing the bottom plate .................................................................................................................. 5-5
Figure 5-6 Removing the left and right support profiles......................................................................................... 5-5
Figure 5-7 Shelf Support Bracket Installation ........................................................................................................ 5-6
Figure 5-8 Perforated Shelf .................................................................................................................................... 5-6
Figure 5-9 AC Power Supply Socket...................................................................................................................... 5-8
Figure 7-1 Control Panel for Fisher Scientific Peltier low temperature incubators.................................................. 7-1
Figure 10-1 Door Seal Replacement ...................................................................................................................... 10-4
List of Figures

List of Figures
ii Peltier Low Temp. Incubators Fisher Scientific

Fisher Scientific Peltier Low Temp. Incubators 1-1
1
Safety Notes
Basic Operating Precautions
These operating instructions describe Fisher Scientific low temperature incubators.
Fisher Scientific low temperature incubators have been manufactured to the latest state of the
art and have been tested thoroughly for flawless functioning prior to shipping. However, the
low temperature incubator may present potential hazards, particularly if it is operated by
inadequately trained personnel or if it is not used in accordance with the intended purpose.
Therefore, the following must be observed for the sake of accident prevention:
• Never step into the unit.
• Fisher Scientific low temperature incubators must be operated by adequately trained and
authorized professional personnel.
• Fisher Scientific low temperature incubators must not be operated unless these operating
instructions have been fully read and understood.
• The present operating instructions, applicable safety data sheets, plant hygiene guidelines
and the corresponding technical rules issued by the operator shall be used to create written
procedures targeted at personnel working with the subject matter device, detailing:
• the decontamination measures to be employed for the low temperature incubator and
the accessories used with it,
• the safety precautions to be taken when processing specific agents,
• the measures to be taken in case of accidents.
• Repair work on the low temperature incubator must be carried out only by trained and
authorized expert personnel.
• The contents of these operating instructions are subject to change at any time without
further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the low temperature incubator so that safety
instructions and important information are always accessible.
• Should you encounter problems that are not detailed adequately in these operating
instructions, please contact Fisher Scientific International, LLC immediately for your own
safety.

Safety Notes
Operational Safety Rules
1-2 Peltier Low Temp. Incubators Fisher Scientific
Operational Safety Rules
The following rules must be heeded when working with Fisher Scientific low temperature
incubators:
• Observe the sample weight limits specified for your Fisher Scientific low temperature
incubator as a whole and its shelving in particular; see “Technical Data” on page 13-1.
• Arrange the samples evenly throughout the work space, making sure not to place them too
closely to the interior walls to ensure a uniform temperature distribution.
• Do not load your Fisher Scientific low temperature incubator with substances that exceed
the capabilities of the available lab apparatus and Personal Protection Equipment to
provide sufficient degrees of protection to users and third parties.
• Check the door seal every six months for proper sealing performance and possible damage.
• Do not process any samples containing hazardous chemical substances that may be
released into the ambient air through defective seals or may cause corrosion or other
defects on parts of the Fisher Scientific low temperature incubator.
• The tempering of defined substances or materials with higher moisture content can result
in increased condensation in the chamber. Measures on page 6-2 must be observed.
Warranty
Fisher Scientific International, LLC warrants the operational safety and functions of the
Fisher Scientific low temperature incubators only under the condition that:
• the low temperature incubator is operated and serviced exclusively in accordance with its
intended purpose and as described in these operating instructions,
• the low temperature incubator is not modified,
• only original spare parts and accessories that have been approved by Thermo Scientific are
used (third-party spares without Fisher Scientific International, LLC approval void the
limited warranty),
• inspections and maintenance are performed at the specified intervals,
• an operation verification test is performed after each repair activity.
The warranty is valid from the date of delivery of the low temperature incubator to the
customer.

Safety Notes
Explanation of Safety Information and Symbols
Fisher Scientific Peltier Low Temp. Incubators 1-3
Explanation of Safety Information and Symbols
Safety Notes and Symbols Used Throughout These Operating
Instructions
Indicates a hazardous situation which, if not avoided, will result
in death or serious injuries.


Indicates a hazardous situation which, if not avoided, could result
in death or serious injuries.
Indicates a situation which, if not avoided, could result in damage to
equipment or property.
Is used for useful hints and information regarding the application.

Safety Notes
Explanation of Safety Information and Symbols
1-4 Peltier Low Temp. Incubators Fisher Scientific
Additional Symbols for Safety Information
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
Biological hazard!
Contamination hazard!
Danger of tipping!

Safety Notes
Explanation of Safety Information and Symbols
Fisher Scientific Peltier Low Temp. Incubators 1-5
Symbols on the low temperature incubator

Observe operating instructions

Mark of conformity USA/Canada

120 Volts AC power socket

Fuse 2 A, slow blow, high breaking current, 250 volt

Alarm contact

Hot surfaces!

RS 232 interface

Safety Notes
Intended Purpose
1-6 Peltier Low Temp. Incubators Fisher Scientific
Intended Purpose
Intended Purpose of the low temperature incubator
Fisher Scientific low temperature incubators are laboratory equipment for tempering (cooling
and heating) of defined substances and materials. They serve for the preparation and
cultivation of cell and tissue cultures. The devices employ precision temperature control for
simulating the particular physiological environment for these cultures.
Fisher Scientific incubators are for professional use only.
Incorrect Use
To avoid the risk of explosion do not load the low temperature incubator with tissue, material,
or liquids that:
• are easily flammable or explosive,
• release vapor or dust that forms combustible or explosive mixtures when exposed to air,
• release poisons,
• do not pour any liquids on the bottom of the interior surface or into a collecting basin
inside the unit.
• release dust
• exhibit exothermic reactions
• are pyrotechnical substances
• refrain also from pouring any liquids onto the internal base plate or inserting bowls filled
with liquids into the sample compartment.
Standards and Directives
The low temperature incubator complies with the following standards and guidelines:
• IEC EN 61010 - 1, IEC EN 61010 - 2 - 010
• Low Voltage Directive 2014/35/EC
• EMC Directive 2014/30/EC
• China EEP Hazardous Substances Information
http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html.
Additionally, the low temperature incubator is in compliance with many other international
standards, regulations and directives not listed here. Should you have any questions regarding
compliance with national standards, regulations and directives applicable for your country,
please contact your Fisher Scientific sales organization.

Fisher Scientific Peltier Low Temp. Incubators 2-1
2
Delivery of the low temperature incubator
Packaging
Fisher Scientific low temperature incubators are delivered in a rugged packaging box. All
packaging materials can be separated and are reusable:
Packaging materials
Packaging carton: Recycled paper
Foam elements: Styrofoam (CFC-free and HFC-free)
Pallet: Chemically untreated wood
Packaging film: Polyethylene
Packaging ribbons: Polypropylene
Acceptance Inspection
After the low temperature incubator has been delivered, check the delivery immediately for:
• completeness,
• possible damage.
If components are missing or damage is found on the low temperature incubator or the
packaging, in particular damage caused by humidity and/or water, please notify the carrier as
well as Thermo Scientific Technical Support immediately.
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere
on the device, take all necessary precautions to protect
personnel handling the low temperature incubator. For example,
have them wear protective gloves and other personal protection
equipment.

Delivery of the low temperature incubator
Scope of Supply
2-2 Peltier Low Temp. Incubators Fisher Scientific
Scope of Supply
Low Temp. Incubators
Quantity of components supplied (pieces) Low Temp.
Incubator
178L
Low Temp.
Incubator
381L
Perforated shelves 2 2
Support rail for shelf table-top low temperature incubators 4 2
Shelf support 4 4
Power cord 1 1
Clip springs for table-top low temperature incubators 4 2
Plug for standard access port 1 1
Anti-tilt anchor 1 1
Operating manual 1 1
Condensate water dish 1 2

Fisher Scientific Peltier Low Temp. Incubators 3-1
3
Installation
Ambient Conditions
Location Requirements
The low temperature incubator must only be operated in a location that meets all of the
ambient condition requirements listed below:
• Installation location indoors in dry areas free from drafts.
• The dust burden may not exceed the contamination category 2 based on EN 61010-1.
Using the low temperature incubator in an atmosphere with electrically conductive dust is
prohibited.
• The minimal distance to adjacent surfaces must be observed on all sides (see Section
“Space Requirement” on page 3-3)
• The operating room must be equipped with appropriate ventilation.
• Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the low
temperature incubator.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of
the low temperature incubator and its accessories (particularly if two devices are stacked).
• The electrical circuitry of the low temperature incubator has been designed for an
operating height of up to 2000 m above sea level.
• If a high-voltage test is to be performed on the unit, it must first be heated for around 30
minutes at 50°C.
• The ambient temperature must be within a range of +18 °C to +28 °C (64.4 °F to 82 °F).
• Avoid direct exposure to sunlight.
• Devices that produce excessive amounts of heat must not be placed near the low
temperature incubator.
• Place the low temperature incubator on a floor stand (option; to be ordered separately),
never on the lab floor. This prevents the penetration of dust or dirt into the device.
• Power line voltage variations must not exceed ±10 % of the nominal voltage.
• Transient surges must lie within the range of levels that normally occur in the power
supply system. The impulse withstand voltage based on surge category II of
IEC 60364-4-443 shall be applied at the nominal voltage level.

Installation
Intermediate Storage
3-2 Peltier Low Temp. Incubators Fisher Scientific
• Relative humidity up to 70%, non condensing, at an ambient temperature of 28°C.
• Should condensation exist, wait until the moisture has evaporated completely before
connecting the low temperature incubator to a power source and powering up.
Humidity
After transport or storing under humid conditions a drying-out
process must be performed. During the drying-out process the
equipment cannot be assumed to meet all the safety
requirements of the IEC 61010-2-010 standard. The drying-out
period is xx hours.
• Consider installing one dedicated upstream circuit breaker per low temperature incubator
to avoid multiple device failures in case of an electrical fault.
Contamination hazard
Do not place the low temperature incubator directly on the lab
floor, but mount it on the floor stand or on a lab work surface
(option; to be ordered separately). Contaminants, such as
bacteria, viruses, fungi, prions, and other biological substances
may use the open door to migrate easily from the floor into the
low temperature incubator’s work space.
Intermediate Storage
When the low temperature incubator is placed in intermediate storage, which is permissible
for a maximum of four weeks, make sure that the ambient temperature is between 20 °C to
60 °C (68 °F to 140 °F) and the maximum relative humidity does not exceed 90%,
non-condensing.
Room Ventilation
Heat dissipating from the low temperature incubator during continuous operation may cause
a change in the room climate.
• Therefore, the low temperature incubator must only be installed in rooms with sufficient
ventilation.
• Do not install the low temperature incubator in room recesses without ventilation.
• When several devices are to be placed in the same room, additional ventilation may have
to be provided as necessary.
• To avoid any impact of the heat dissipated by the low temperature incubator on the
ambient climate the room must be vented by means of a laboratory-grade ventilation
system that complies with applicable local and national health and safety regulations and
has sufficient capacity.
• If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power supply to mitigate the impact of
over-temperature scenarios.

Installation
Room Ventilation
Fisher Scientific Peltier Low Temp. Incubators 3-3
Space Requirement
When installing the low temperature incubator, make sure that the installation and supply
connections remain freely accessible.
The specified side clearances represent minimum distances.
Table-top low temperature incubators
Figure 3-1 Table-top Peltier low temperature incubator, dimensions and required
clearances
Table 3-1 Peltier low temp. incubator Dimensions
Model A (mm/inch) B (mm/inch) C (mm/inch) D (mm/inch)
Isotemp 178L
low temp. Incub.
640/25.2 738/29.1 920/36.2 650/25.6
* Depth of handle /display (66 mm/2.6 in) not included in overall depth specified; height of adjustable feet (36 mm/1.4 in)
not included in overall height specified.
Table 3-2 Required Clearances
E(mm/inch) F(mm/inch) H(mm/inch) I(mm/inch)
130/5,1 150/5,9 200/7,9 300/11,8

Installation
Room Ventilation
3-4 Peltier Low Temp. Incubators Fisher Scientific
Floor Stand Peltier low temp. incubator
381 liter unit
Figure 3-2 Floor stand Peltier low temperature incubators, dimensions and required
clearances
Table 3-3 Peltier low temp. incubator Dimensions
Model A (mm/inch) B (mm/inch) C (mm/inch) D (mm/inch)
Isotemp 381L
low temp. Incub.
755/29.7 770/30.3 1655/65.2 810/31.9
*Depth of handle /display (66 mm/2.6 in) not included in overall depth specified. Width of hinge (23 mm) not included in
overall width.
Table 3-4 Required Clearances
E(mm/inch) F(mm/inch) H(mm/inch) I(mm/inch)
150/5,9 150/5,9 360/14,2 200/7,9