Fisher Scientific Isotemp Series User manual

i-ii Gravity Convection Incubators Fisher Scientific
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English version of these operating instructions is binding.
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Thermo Fisher Scientific
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Fisher Scientific Gravity Convection Incubators i
C
Chapter 1 Safety Notes..................................................................................................................... 1-1
Basic Operating Precautions ............................................................................................................... 1-1
Operational Safet Rules .................................................................................................................... 1-2
Warrant ............................................................................................................................................ 1-2
Explanation of Safet Information and S mbols ................................................................................. 1-3
Safet Notes and S mbols Used Throughout These Operating Instructions ................................... 1-3
Additional S mbols for Safet Information..................................................................................... 1-4
S mbols on the Incubator............................................................................................................... 1-5
Intended Purpose of the Incubator ..................................................................................................... 1-6
Correct Use .................................................................................................................................... 1-6
Incorrect Use .................................................................................................................................. 1-6
Standards and Directives .................................................................................................................... 1-6
Chapter 2 Delivery of the Incubator................................................................................................. 2-1
Packaging ........................................................................................................................................... 2-1
Acceptance Inspection ........................................................................................................................ 2-1
Scope of Suppl .................................................................................................................................. 2-2
Chapter 3 Installation........................................................................................................................ 3-1
Ambient Conditions........................................................................................................................... 3-1
Location Requirements................................................................................................................... 3-1
Intermediate Storage........................................................................................................................... 3-2
Room Ventilation............................................................................................................................... 3-2
Space Requirements............................................................................................................................ 3-3
Table-top units............................................................................................................................... 3-3
Installing the Anti-tilt Anchor ............................................................................................................ 3-5
Chapter 4 Product Description ........................................................................................................ 4-1
Gravit Convection Incubator Overview ............................................................................................ 4-1
Forced Air Incubator Overview .......................................................................................................... 4-4
Safet Devices..................................................................................................................................... 4-7
Work Space Atmosphere .................................................................................................................... 4-7
Sensing and Control S stem ............................................................................................................... 4-7
Data Communications & Alarm Interface.......................................................................................... 4-8
RS-232 Interface............................................................................................................................. 4-8
AC Power Socket............................................................................................................................ 4-9
Fuses............................................................................................................................................... 4-9
Work Space Components................................................................................................................... 4-9
Inner Chamber ............................................................................................................................... 4-9
Access Port ..................................................................................................................................... 4-9
Shelf S stem ..................................................................................................................................... 4-10
Contents

Contents
ii Gravity Convection Incubators Fisher Scientific
Chapter 5 Start-up............................................................................................................................. 5-1
Installing the Shelf S stem for Table-top Units .................................................................................. 5-1
Initial Installation ............................................................................................................................... 5-1
Installing the Perforated Shelves ......................................................................................................... 5-2
Preparing the Work Space .................................................................................................................. 5-3
Installation of the Support Rails (onl for table-top Units) ............................................................. 5-3
Installing the Shelf Support Brackets .............................................................................................. 5-4
Installing the Perforated Shelves ..................................................................................................... 5-4
Installing/Removing air baffles........................................................................................................ 5-5
Levelling the Table-top Incubator Unit .......................................................................................... 5-6
Connecting Power.............................................................................................................................. 5-6
Connecting to the Power Suppl Source......................................................................................... 5-7
Connecting the RS-232 Interface ....................................................................................................... 5-8
Chapter 6 Operation.......................................................................................................................... 6-1
Preparing the Incubator...................................................................................................................... 6-1
Starting Operation.............................................................................................................................. 6-1
Chapter 7 Handling and Control ...................................................................................................... 7-1
Powering Up ...................................................................................................................................... 7-5
Switching the Incubator Off / Powering Down .................................................................................. 7-5
Temperature Set Value ....................................................................................................................... 7-6
Timer ................................................................................................................................................. 7-7
Stopping a Timer............................................................................................................................ 7-8
Settings............................................................................................................................................. 7-10
Error Log...................................................................................................................................... 7-10
Calibration ................................................................................................................................... 7-11
Temperature Displa Unit............................................................................................................ 7-12
Chapter 8 Shut-down ........................................................................................................................ 8-1
Shutting the Incubator Down............................................................................................................. 8-1
Chapter 9 Cleaning and Disinfection............................................................................................... 9-1
Cleaning............................................................................................................................................. 9-1
Wipe / Spra Disinfection .................................................................................................................. 9-1
Preparing the Manual Wipe/Spra Disinfection ............................................................................. 9-2
Predisinfection.................................................................................................................................... 9-3
Cleaning......................................................................................................................................... 9-3
Final Disinfection........................................................................................................................... 9-3
Chapter 10 Maintenance................................................................................................................... 10-1
Inspections and Checks .................................................................................................................... 10-1
Service Intervals................................................................................................................................ 10-2
Preparing Temperature Calibration .................................................................................................. 10-2
Comparison Measurement Procedure............................................................................................... 10-3
Temperature Calibration Procedure ................................................................................................. 10-3
Replacing the Door Seal ................................................................................................................... 10-4
Replacing the Power Cord................................................................................................................ 10-4
Returns for Repair ............................................................................................................................ 10-5

Contents
Fisher Scientific Gravity Convection Incubators iii
Chapter 11 Disposal...........................................................................................................................11-1
Overview of Materials Used ..............................................................................................................11-1
Chapter 12 Error Codes.....................................................................................................................12-1
Chapter 13 Technical Data................................................................................................................13-1
Chapter 14 Spare Parts and Accessories........................................................................................14-1
Chapter 15 Device Log ......................................................................................................................15-1

Contents
iv Gravity Convection Incubators Fisher Scientific

Fisher Scientific Gravity Convection Incubators i
L
Figure 3-1 Table-top Incubators, dimensions and required clearances.................................................................... 3-3
Figure 3-2 Floor Stand Incubators, dimensions and required clearances................................................................. 3-5
Figure 4-1 IGS060 GC, IGS100 GC and IGS180 GC Front View ............................................................................... 4-2
Figure 4-2 IGS060 GC, IGS100 GC and IGS180 GC Rear View ............................................................................... 4-3
Figure 4-3 IMS060 FA, IMS100 FA and IMS180 FA Front View ............................................................................... 4-5
Figure 4-4 MS060 FA, IMS100 FA and IMS180 FA Rear View.................................................................................. 4-5
Figure 4-7 Sensor S stem (for table-top units) ....................................................................................................... 4-9
Figure 4-9 Signal Interfaces and Power Socket....................................................................................................... 4-10
Figure 4-10 Gravit Convection Shelf S stem........................................................................................................ 4-12
Figure 4-11 Forced Air Shelf S stem...................................................................................................................... 4-12
Figure 5-1 Sliding the Retaining Spring into the Support Rail ............................................................................... 5-1
Figure 5-2 Installing the Shelving .......................................................................................................................... 5-2
Figure 5-3 Support Rail Installation....................................................................................................................... 5-3
Figure 5-4 Shelf Support Installation ..................................................................................................................... 5-4
Figure 5-5 Installing the Perforated Shelves ........................................................................................................... 5-4
Figure 5-6 Removing the bottom plate .................................................................................................................. 5-5
Figure 5-7 Removing the left and right support profiles......................................................................................... 5-5
Figure 5-8 Removing the rear air baffle.................................................................................................................. 5-5
Figure 5-9 AC Power Suppl Socket ...................................................................................................................... 5-7
Figure 7-1 Control Panel for Fisher Scientific Incubators ...................................................................................... 7-1
Figure 10-1 Door Seal Replacement ...................................................................................................................... 10-4
List of Figures

List of Figures
ii Gravity Convection Incubators Fisher Scientific

Fisher Scientific Gravity Convection Incubators 1-1
1
Safety Notes
Basic Operating Precautions
These operating instructions describe Fisher Scientific incubators.
Fisher Scientific incubators have been manufactured to the latest state of the art and have been
tested thoroughl for flawless functioning prior to shipping. However, the incubator ma
present potential hazards, particularl if it is operated b inadequatel trained personnel or if it
is not used in accordance with the intended purpose. Therefore, the following must be
observed for the sake of accident prevention:
• Never step into the unit.
• Fisher Scientific incubators must be operated b adequatel trained and authorized
professional personnel.
• Fisher Scientific incubators must not be operated unless these operating instructions have
been full read and understood.
• The present operating instructions, applicable safet data sheets, plant h giene guidelines
and the corresponding technical rules issued b the operator shall be used to create written
procedures targeted at personnel working with the subject matter device, detailing:
• the decontamination measures to be emplo ed for the incubator and the accessories
used with it,
• the safet precautions to be taken when processing specific agents,
• the measures to be taken in case of accidents.
• Repair work on the incubator must be carried out onl b trained and authorized expert
personnel.
• The contents of these operating instructions are subject to change at an time without
further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the incubator so that safet instructions and
important information are alwa s accessible.
• Should ou encounter problems that are not detailed adequatel in these operating
instructions, please contact Fisher Scientific International, LLC immediatel for our own
safet .

Safety Notes
Operational Safety Rules
1-2 Gravity Convection Incubators Fisher Scientific
Operational Safety Rules
The following rules must be heeded when working with Fisher Scientific incubators:
• Observe the sample weight limits specified for our Fisher Scientific incubator as a whole
and its shelving in particular; see “Technical Data” on page 13-1.
• Do not load the bottom of the interior workspace to avoid the risk of overheating an
samples placed there and to prevent the temperature sensor from being damaged.
• Arrange the samples evenl throughout the work space, making sure not to place them too
closel to the interior walls to ensure a uniform temperature distribution.
• Do not load our Fisher Scientific incubator with substances that exceed the capabilities of
the available lab apparatus and Personal Protection Equipment to provide sufficient
degrees of protection to users and third parties.
• Check the door seal ever six months for proper sealing performance and possible damage.
• Do not process an samples containing hazardous chemical substances that ma be
released into the ambient air through defective seals or ma cause corrosion or other
defects on parts of the Fisher Scientific incubator.
Warranty
Fisher Scientific International, LLC warrants the operational safet and functions of the Fisher
Scientific incubators onl under the condition that:
• the incubator is operated and serviced exclusivel in accordance with its intended purpose
and as described in these operating instructions,
• the incubator is not modified,
• onl original spare parts and accessories that have been approved b Fisher Scientifi care
used (third-part spares without Fisher Scientific International, LLC approval void the
limited warrant ),
• inspections and maintenance are performed at the specified intervals,
• an operation verification test is performed after each repair activit .
The warrant is valid from the date of deliver of the incubator to the customer.

Safety Notes
Explanation of Safety Information and Symbols
Fisher Scientific Gravity Convection Incubators 1-3
Explanation of Safety Information and Symbols
Safety Notes and Symbols Used Throughout These Operating
Instructions
Indicates a hazardous situation which, if not avoided, will result
in death or serious injuries.
Indicates a hazardous situation which, if not avoided, could result
in death or serious injuries.
Indicates a situation which, if not avoided, could result in damage to
equipment or property.
Is used for useful hints and information regarding the application.

Safety Notes
Explanation of Safety Information and Symbols
1-4 Gravity Convection Incubators Fisher Scientific
Additional Symbols for Safety Information
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
Biological hazard!
Contamination hazard!
Danger of tipping!

Safety Notes
Explanation of Safety Information and Symbols
Fisher Scientific Gravity Convection Incubators 1-5
Symbols on the Incubator
Observe operating instructions
Mark of conformity USA/Canada
120 Volts AC power socket

Safety Notes
Intended Purpose of the Incubator
1-6 Gravity Convection Incubators Fisher Scientific
Intended Purpose of the Incubator
Correct Use
Fisher Scientific incubators are laborator devices for preparing and cultivating cell and tissue
cultures. The devices emplo precision temperature control for simulating the specific
ph siological ambient conditions for these cultures.
Fisher Scientific incubators are for professional use onl .
Incorrect Use
To avoid the risk of explosion do not load the incubator with tissue, material, or liquids that:
• are easil flammable or explosive,
• release vapor or dust that forms combustible or explosive mixtures when exposed to air,
• release poisons,
• do not pour an liquids on the bottom of the interior surface or into a collecting basin
inside the unit.
• release dust
• exhibit exothermic reactions
• are p rotechnical substances
• refrain also from pouring an liquids onto the internal base plate or inserting bowls filled
with liquids into the sample compartment.
Standards and Directives
The incubator complies with the following standards and guidelines:
• IEC EN 61010 - 1, IEC EN 61010 - 2 - 010
Additionall , the incubator is in compliance with man other international standards,
regulations and directives not listed here. Should ou have an questions regarding compliance
with national standards, regulations and directives applicable for our countr , please contact
our Fisher Scientific sales organization.

Fisher Scientific Gravity Convection Incubators 2-1
2
Delivery of the Incubator
Packaging
Fisher Scientific incubators are delivered in a rugged packaging box. All packaging materials
can be separated and are reusable:
Packaging materials
Packaging carton: Rec cled paper
Foam elements: St rofoam (CFC-free)
Pallet: Chemicall untreated wood
Packaging film: Pol eth lene
Packaging ribbons: Pol prop lene
Acceptance Inspection
After the incubator has been delivered, check the deliver immediatel for:
• completeness,
• possible damage.
If components are missing or damage is found on the incubator or the packaging, in particular
damage caused b humidit and/or water, please notif the carrier as well as Thermo Fisher
Scientific Technical Support immediatel .
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere
on the device, take all necessary precautions to protect
personnel handling the incubator. For example, have them wear
protective gloves and other personal protection equipment.

Delivery of the Incubator
Scope of Supply
2-2 Gravity Convection Incubators Fisher Scientific
Scope of Supply
Incubators
Quantity of components supplied (pieces) Gravity Convection
Incubators Forced Air Incubators
Perforated shelves 2 2
Support rail for shelf table-top incubators 4 2
Shelf support 4 4
Power cord 1 1
Clip springs for table-top incubators 4 2
Plug 1 1
Anti-tilt anchor 1 1
Operating manual 1 1

Fisher Scientific Gravity Convection Incubators 3-1
3
Installation
Ambient Conditions
Location Requirements
The incubator must onl be operated in a location that meets all of the ambient condition
requirements listed below:
• Installation location indoors in dr areas free from drafts.
• The dust burden ma not exceed the contamination categor 2 based on EN 61010-1.
Using the incubator in an atmosphere with electricall conductive dust is prohibited.
• The minimal distance to adjacent surfaces must be observed on all sides (see Section
“Space Requirement” on page 3-3)
• The operating room must be equipped with appropriate ventilation.
• Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the
incubator.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of
the incubator and its accessories (particularl if two devices are stacked).
• The electrical circuitr of the incubator has been designed for an operating height of up to
2000 m above sea level.
• Relative humidit up to 80% (maximum; preferabl 60-70%), non condensing.
• Should condensation exist, wait until the moisture has evaporated completel before
connecting the incubator to a power source and powering up.
• If a high-voltage test is to be performed on the unit, it must first be heated for around 30
minutes at 75°C.
• The ambient temperature must be within a range of +18 °C to +32 °C (64.4 °F to 89.6 °F).
• Avoid direct exposure to sunlight.
• Devices that produce excessive amounts of heat must not be placed near the incubator.
• Place the incubator on a floor stand (option; to be ordered separatel ), never on the lab
floor. This prevents the penetration of dust or dirt into the device.
• Power line voltage variations must not exceed ±10 % of the nominal voltage.

Installation
Intermediate Storage
3-2 Gravity Convection Incubators Fisher Scientific
• Transient surges must lie within the range of levels that normall occur in the power
suppl s stem. The impulse withstand voltage based on surge categor II of
IEC 60364-4-443 shall be applied at the nominal voltage level.
• Consider installing one dedicated upstream circuit breaker per incubator to avoid multiple
device failures in case of an electrical fault.
Intermediate Storage
When the incubator is placed in intermediate storage, which is permissible for a maximum of
four weeks, make sure that the ambient temperature is between 20 °C to 60 °C (68 °F to
140 °F) and the maximum relative humidit does not exceed 90%, non-condensing.
Room Ventilation
Heat dissipating from the incubator during continuous operation ma cause a change in the
room climate.
• Therefore, the incubator must onl be installed in rooms with sufficient ventilation.
• Do not install the incubator in room recesses without ventilation.
• When several devices are to be placed in the same room, additional ventilation ma have
to be provided as necessar .
• To avoid an impact of the heat dissipated b the incubator on the ambient climate the
room must be vented b means of a laborator -grade ventilation s stem that complies
with applicable local and national health and safet regulations and has sufficient capacit .
• If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power suppl to mitigate the impact of
overtemperature scenarios.
Contamination hazard
Do not place the incubator directly on the lab floor, but mount it
on the floor stand or on a lab work surface (option; to be ordered
separately). Contaminants, such as bacteria, viruses, fungi,
prions, and other biological substances may use the open door to
migrate easily from the floor into the incubator’s work space.

Installation
Room Ventilation
Fisher Scientific Gravity Convection Incubators 3-3
Table-top incubators
Figure 3-1 Table-top incubators, dimensions and required clearances
* Depth of handle /displa (66 mm/2.6 in) not included in overall depth specified; height of adjustable feet (36 mm/1.4 in)
not included in overall height specified.
Table 3-1 Incubator Dimensions
Model A (mm/inch) B (mm/inch) C (mm/inch) D (mm/inch)
Isotemp 60L
Incbtr Grvity 530/20.1 565/22.2 720/28.3 540/21.3
Isotemp 100L
Incbtr Grvity 640/25.2 565/22.2 820/32.3 650/25.6
Isotemp 180L
Incbtr Grvity 640/25.2 738/29.1 920/36.2 650/25.6
Isotemp 60L
Incbtr FA 530/20.1 565/22.2 720/28.3 540/21.3
Isotemp 100L
Incbtr FA 640/25.2 565/22.2 820/32.3 650/25.6
Isotemp 180L
Incbtr FA 640/25.2 738/29.1 920/36.2 650/25.6
Table 3-2 Required Clearances
E (mm/inch) F (mm/inch) G (mm/inch) H (mm/inch)
80/3.1 50/2.0 200/7.9 300/11.8

Installation
Transport
3-4 Gravity Convection Incubators Fisher Scientific
Transport
Table-top incubators
For transport, do not lift the incubator using the doors or components attached to the
incubator as lift points.
Figure 3-2 Lift Points
Heavy loads! Lift with care!
To avoid injury through physical strain, such as strain traumata
and slipped discs, do not attempt to lift the incubator alone!
To avoid injury through dropped loads, be sure to wear Personal
Protection Equipment, such as safety shoes, when lifting the
incubator.
To avoid crushing your fingers or hands (particularly in a closing
door) or damaging the incubator, do not use any other lift points
than those indicated in the illustration above.