Fisher Scientific Isotemp 60L Incbtr Grvity 120V User manual

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© 2018 Fisher Scientific. All rights reserved.
This Instruction Manual is copyright protected. Rights resulting thereof, particularly reprint, photomechanical or digital
post-processing or reproduction, even in part, are only allowed with the written consent of Fisher Scientific.
This regulation does not apply to reproductions for in-plant use.
The contents of this document are subject to change without notice. Concerning translations into foreign languages, the
English version of these operating instructions is binding.
Trademarks
All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
Fisher Scientific Company L.L.C
275 Aiken Road
Asheville, NC 28804
United States
Fisher Scientific provides this document to its customers with a product purchase to use in the product operation. This
document is copyright protected This document is copyright protected and any reproduction of the whole or any part
of this document is strictly prohibited, except with the written authorization of Fisher Scientific.
The content of this document are subject to change without notice.
All technical information in this document is for reference purposes only. System configurations and specifications in
this document supersede all previous information received by the purchaser.
This document is not part of any sales contract between Fisher Scientific and a purchaser. This document shall in no
way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all
conflicting information between the two documents.

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Basic Operating Precautions ............................................................................................................... 1-1
Operational Safety Rules .................................................................................................................... 1-2
Warranty ............................................................................................................................................ 1-2
Explanation of Safety Information and Symbols ................................................................................. 1-3
Intended Purpose of the Incubator ..................................................................................................... 1-6
Standards and Directives .................................................................................................................... 1-6
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Packaging ........................................................................................................................................... 2-1
Acceptance Inspection ........................................................................................................................ 2-1
Scope of Supply.................................................................................................................................. 2-2
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Ambient Conditions........................................................................................................................... 3-1
Intermediate Storage........................................................................................................................... 3-2
Room Ventilation............................................................................................................................... 3-2
Transport ........................................................................................................................................... 3-4
Installing the Anti-tilt Anchor ............................................................................................................ 3-5
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Gravity Convection Incubator Overview ............................................................................................ 4-1
Forced Air Incubator Overview .......................................................................................................... 4-4
Safety Devices..................................................................................................................................... 4-6
Work Space Atmosphere .................................................................................................................... 4-6
Sensing and Control System............................................................................................................... 4-7
Data Communications & Alarm Interface.......................................................................................... 4-8
Work Space Components................................................................................................................... 4-9
Shelf System ..................................................................................................................................... 4-10
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Installing the Shelf System for Table-top Units .................................................................................. 5-1
Initial Installation ............................................................................................................................... 5-1
Installing the Perforated Shelves ......................................................................................................... 5-2
Preparing the Work Space .................................................................................................................. 5-3
Connecting Power .............................................................................................................................. 5-6
Connecting the RS-232 Interface ....................................................................................................... 5-8
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Preparing the Incubator...................................................................................................................... 6-1
Starting Operation.............................................................................................................................. 6-1

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Powering Up ...................................................................................................................................... 7-5
Switching the Oven Off / Powering Down......................................................................................... 7-5
Temperature Set Value ....................................................................................................................... 7-6
Timer ................................................................................................................................................. 7-7
Settings............................................................................................................................................... 7-9
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Shutting the Incubator Down............................................................................................................. 8-1
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Cleaning............................................................................................................................................. 9-1
Wipe / Spray Disinfection .................................................................................................................. 9-1
Predisinfection.................................................................................................................................... 9-3
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Inspections and Checks .................................................................................................................... 10-1
Service Intervals................................................................................................................................ 10-2
Preparing Temperature Calibration .................................................................................................. 10-2
Comparison Measurement Procedure............................................................................................... 10-3
Temperature Calibration Procedure ................................................................................................. 10-3
Replacing the Door Seal ................................................................................................................... 10-4
Replacing the Power Cord................................................................................................................ 10-4
Returns for Repair ............................................................................................................................ 10-5
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Overview of Materials Used.............................................................................................................. 11-1
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Figure 3-1 Table-top incubators, dimensions and required clearances .................................................................... 3-3
Figure 3-2 Lift Points............................................................................................................................................. 3-4
Figure 4-1 Isotemp 60L/100L/180L Incbtr Grvity Front View.............................................................................. 4-2
Figure 4-2 Isotemp 60L/100L/180L Incbtr Grvity Rear View ............................................................................... 4-3
Figure 4-3 Isotemp 60L/100L/180L Incbtr FA Front View ................................................................................... 4-5
Figure 4-4 Isotemp 60L/100L/180L Incbtr FA Rear View..................................................................................... 4-6
Figure 4-5 Sensor System (for table-top incubators)............................................................................................... 4-7
Figure 4-6 Sensor System (for floor stand incubators) ............................................................................................ 4-7
Figure 4-7 Signal Interfaces and Power Socket ....................................................................................................... 4-8
Figure 4-8 Gravity Convection Shelf System.......................................................................................................... 4-10
Figure 4-9 Forced Air Shelf System........................................................................................................................ 4-11
Figure 5-1 Sliding the Retaining Spring into the Support Rail ............................................................................... 5-1
Figure 5-2 Installing the Shelving .......................................................................................................................... 5-2
Figure 5-3 Forced Air Shelf System........................................................................................................................ 5-2
Figure 5-4 Support Rail Installation....................................................................................................................... 5-3
Figure 5-5 Shelf Support Installation ..................................................................................................................... 5-4
Figure 5-6 Installing the Perforated Shelves ........................................................................................................... 5-4
Figure 5-7 Removing the bottom plate .................................................................................................................. 5-5
Figure 5-8 Removing the left and right support profiles......................................................................................... 5-5
Figure 5-9 Removing the rear air baffle 85911-430................................................................................................ 5-6
Figure 5-10 AC Power Supply Socket .................................................................................................................... 5-7
Figure 7-1 Control Panel for gravity convection and forced air ovens .................................................................... 7-1
Figure 10-1 Door Seal Replacement ...................................................................................................................... 10-4

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These operating instructions describe Fisher Scientific incubators.
Fisher Scientific incubators have been manufactured to the latest state of the art and have been
tested thoroughly for flawless functioning prior to shipping. However, the incubator may
present potential hazards, particularly if it is operated by inadequately trained personnel or if it
is not used in accordance with the intended purpose. Therefore, the following must be
observed for the sake of accident prevention:
• Never step into the unit.
• Fisher Scientific incubators must be operated by adequately trained and authorized
professional personnel.
• Fisher Scientific incubators must not be operated unless these operating instructions have
been fully read and understood.
• The present operating instructions, applicable safety data sheets, plant hygiene guidelines
and the corresponding technical rules issued by the operator shall be used to create written
procedures targeted at personnel working with the subject matter device, detailing:
• the decontamination measures to be employed for the incubator and the accessories
used with it,
• the safety precautions to be taken when processing specific agents,
• the measures to be taken in case of accidents.
• Repair work on the incubator must be carried out only by trained and authorized expert
personnel.
• The contents of these operating instructions are subject to change at any time without
further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the incubator so that safety instructions and
important information are always accessible.
• Should you encounter problems that are not detailed adequately in these operating
instructions, please contact Fisher Scientific International, LLC immediately for your own
safety.

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The following rules must be heeded when working with Fisher Scientific incubators:
• Observe the sample weight limits specified for your Fisher Scientific incubator as a whole
and its shelving in particular; see Chapter 13, “Technical Data.” .
• Do not load the bottom of the interior workspace to avoid the risk of overheating any
samples placed there and to prevent the temperature sensor from being damaged.
• Arrange the samples evenly throughout the work space, making sure not to place them too
closely to the interior walls to ensure a uniform temperature distribution.
• Do not load your Fisher Scientific incubator with substances that exceed the capabilities of
the available lab apparatus and Personal Protection Equipment to provide sufficient
degrees of protection to users and third parties.
• Check the door seal every six months for proper sealing performance and possible damage.
• Do not process any samples containing hazardous chemical substances that may be
released into the ambient air through defective seals or may cause corrosion or other
defects on parts of the Fisher Scientific incubator.
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Fisher Scientific International, LLC warrants the operational safety and functions of the Fisher
Scientific incubators only under the condition that:
• the incubator is operated and serviced exclusively in accordance with its intended purpose
and as described in these operating instructions,
• the incubator is not modified,
• only original spare parts and accessories that have been approved by Fisher Scientific care
used (third-party spares without Fisher Scientific International, LLC approval void the
limited warranty),
• inspections and maintenance are performed at the specified intervals,
• an operation verification test is performed after each repair activity.
The warranty is valid from the date of delivery of the incubator to the customer.

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Fisher Scientific incubators are laboratory devices for preparing and cultivating cell and tissue
cultures. The devices employ precision temperature control for simulating the specific
physiological ambient conditions for these cultures.
Fisher Scientific incubators are for professional use only.
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To avoid the risk of explosion do not load the incubator with tissue, material, or liquids that:
• are easily flammable or explosive,
• release vapor or dust that forms combustible or explosive mixtures when exposed to air,
• release poisons,
• do not pour any liquids on the bottom of the interior surface or into a collecting basin
inside the unit.
• release dust
• exhibit exothermic reactions
• are pyrotechnical substances
• refrain also from pouring any liquids onto the internal base plate or inserting bowls filled
with liquids into the sample compartment.
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The incubator complies with the following standards and guidelines:
• IEC EN 61010 - 1, IEC EN 61010 - 2 - 010
Additionally, the incubator is in compliance with many other international standards,
regulations and directives not listed here. Should you have any questions regarding compliance
with national standards, regulations and directives applicable for your country, please contact
your Fisher Scientific sales organization.

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Fisher Scientific incubators are delivered in a rugged packaging box. All packaging materials
can be separated and are reusable:
Packaging materials
Packaging carton: Recycled paper
Foam elements: Styrofoam (CFC-free)
Pallet: Chemically untreated wood
Packaging film: Polyethylene
Packaging ribbons: Polypropylene
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After the incubator has been delivered, check the delivery immediately for:
• completeness,
• possible damage.
If components are missing or damage is found on the incubator or the packaging, in particular
damage caused by humidity and/or water, please notify the carrier as well as Fisher Scientific
Technical Support immediately.
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Perforated shelves 2 2
Support rail for shelf table-top incubators 4 2
Shelf support 4 4
Power cord 1 1
Clip springs for table-top incubators 4 2
Plug 1 1
Anti-tilt anchor 1 1
Operating manual 1 1

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The incubator must only be operated in a location that meets all of the ambient condition
requirements listed below:
• Installation location indoors in dry areas free from drafts.
• The dust burden may not exceed the contamination category 2 based on EN 61010-1.
Using the incubator in an atmosphere with electrically conductive dust is prohibited.
• The minimal distance to adjacent surfaces must be observed on all sides. (See Table 3-2 on
page 3-3)
• The operating room must be equipped with appropriate ventilation.
• Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the
incubator.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of
the incubator and its accessories (particularly if two devices are stacked).
• The electrical circuitry of the incubator has been designed for an operating height of up to
2000 m above sea level.
• Relative humidity up to 80% (maximum; preferably 60-70%), non condensing.
• Should condensation exist, wait until the moisture has evaporated completely before
connecting the incubator to a power source and powering up.
• If a high-voltage test is to be performed on the unit, it must first be heated for around 30
minutes at 75°C (167 °F).
• The ambient temperature must be within a range of +18 °C to +32 °C (64.4 °F to 89.6 °F).
• Avoid direct exposure to sunlight.
• Devices that produce excessive amounts of heat must not be placed near the incubator.
• Place the incubator on a floor stand (option; to be ordered separately), never on the lab
floor. This prevents the penetration of dust or dirt into the device.
• Power line voltage variations must not exceed ±10 % of the nominal voltage.

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• Transient surges must lie within the range of levels that normally occur in the power
supply system. The impulse withstand voltage based on surge category II of
IEC 60364-4-443 shall be applied at the nominal voltage level.
• Consider installing one dedicated upstream circuit breaker per incubator to avoid multiple
device failures in case of an electrical fault.
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When the incubator is placed in intermediate storage, which is permissible for a maximum of
four weeks, make sure that the ambient temperature is between 20 °C to 60 °C (68 °F to
140 °F) and the maximum relative humidity does not exceed 90%, non-condensing.
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Heat dissipating from the incubator during continuous operation may cause a change in the
room climate.
• Therefore, the incubator must only be installed in rooms with sufficient ventilation.
• Do not install the incubator in room recesses without ventilation.
• When several devices are to be placed in the same room, additional ventilation may have
to be provided as necessary.
• To avoid any impact of the heat dissipated by the incubator on the ambient climate the
room must be vented by means of a laboratory-grade ventilation system that complies
with applicable local and national health and safety regulations and has sufficient capacity.
• If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power supply to mitigate the impact of over
temperature scenarios.

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* Depth of handle /display (66 mm/2.6 in) not included in overall depth specified; height of adjustable feet (36 mm/1.4 in)
not included in overall height specified.
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E (mm/inch) F (mm/inch) G (mm/inch) H (mm/inch)
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For transport, do not lift the incubator using the doors or components attached to the
incubator as lift points.
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The anti-tilt anchor secures the device to a solid part of a building. The anti-tilt anchor is to
be mounted on the side opposite of the door hinges.
Bend the fixing tabs of the anti-tilt anchor up on one side and down on the other by an angle
of approx. 90°.
1. Do not use this position if the door is hinged on this side. Right-hand hinges represent the
standard configuration.
2. Preferred position.
3. Alternative position. Do not use if the door is hinged on this side.
Remove the bracket screws. Use the preferred position, if possible.
Fix the anti-tilt anchor with the bracket side down to the unit.
Position the unit with the anti-tilt anchor to in an angle of approx. 90° +/- 20%.
Take care that the stacking feet of the unit are still in correct place on the lower unit or on the
stacking adapter.
Fix the anti-tilt anchor to a solid part of the building.
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Fisher Scientific floor stand incubators must always be attached to the
wall using two (2) retaining brackets on the outer left and right side on
the back of the unit.

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Remove the screws.
Attach the end of the retaining bracket that is facing downward to the unit.
Align the device at roughly 90°, +/-20° to the retaining bracket.
Affix the retaining bracket to the wall.
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Additionally, the following caution notes must be heeded at all times:
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