Fisher Scientific FB300 User manual

FB300
Electrophoresis
Power Supply
7607850 • 9/13/06

Introduction..........................................................................................................................................................3
Features........................................................................................................................................................3
Safety Information ..............................................................................................................................................4
Alert Signals..................................................................................................................................................4
Specifications ......................................................................................................................................................5
AC Input Power ............................................................................................................................................5
Environmental ..............................................................................................................................................5
DC Output Power..........................................................................................................................................5
Unpacking and Installation ..................................................................................................................................6
Operation ............................................................................................................................................................7
Location ........................................................................................................................................................7
Using the Power Supply................................................................................................................................7
Constant Voltage Operations ........................................................................................................................8
Constant Current Operations........................................................................................................................9
Timed Operations ........................................................................................................................................9
Automatic Power-Fail Restart ....................................................................................................................10
Maintenance......................................................................................................................................................11
Cleaning......................................................................................................................................................11
Replacing a Fuse........................................................................................................................................11
Tr oubleshooting ................................................................................................................................................12
Replacement Parts............................................................................................................................................13
Accessories ................................................................................................................................................13
Replacement Parts ....................................................................................................................................13
Compliance ......................................................................................................................................................15
Warranty ............................................................................................................................................................16
2
Ta ble of Contents

The FB300 power supply is designed to provide constant
voltage or constant current output to apparatus used in
electrophoresis applications. One to three sets of elec-
trophoresis cells can be connected in parallel and run
simultaneously. The FB300 can deliver up to 75W of total
output power. When operating in constant voltage or con-
stant current mode, the power supply automatically limits
the other parameter to either the power supply maximum,
or a lower limit if set by the user. If this non-constant limit
is reached, the power supply will automatically switch
control modes, from constant voltage to constant current,
or vice versa. In this way, the FB300 protects your elec-
trophoresis cells from damaging over power conditions.
The FB300 power supply also provides for timed opera-
tion in either voltage or current modes, and allows an
automatic completion in the event of a power loss if
enabled by the user.
Features
•10-300V, adjustable in 1 volt steps
•4-400mA, adjustable in 1mA steps
•75W maximum output
•Automatic control mode crossover
•0-999 minute timed run
•Automatic restart if loss of AC power (if enabled)
•Bright 3-digit display
3
Introduction

Read and understand this manual completely before
attempting to set up or use this instrument.
This equipment has been designed and tested to conform
to CSA1010 safety standards, as applicable to laboratory
instrumentation. This applies only to the FB300 when
used as specified in the documentation, in its intended
applications, with Fisher Biotech approved electrophoresis
apparatus only. Usage in any other manor may not pro-
vide similar performance or safety protection.
This equipment is provided with a 3-conductor, grounded
AC line cord. The protective earth ground is necessary
for safe operation. Do not use any other AC line cord with
this instrument.
The FB300 is a high voltage power supply capable of
generating dangerous levels of voltage and current during
operation. Exercise caution when working around and
with the electrical connections of this equipment. Always
check electrical connectors, wires, and associated appa-
ratus for any signs of wear or damage before using with
this equipment. Be sure to use only electrophoresis
equipment that is suitably rated for the voltage and cur-
rent capabilities of the FB300 power supply.
The output of the FB300 power supply is intended for
connection to electrically isolated electrophoresis appara-
tus only. Use only with electrically isolated electrophore-
sis apparatus with minimum isolation of 600V. Do not
connect any terminal of the FB300 output to earth
ground. This may impair the safety protection provided by
the equipment, or cause equipment damage.
The high voltage output of the FB300 power supply takes
some amount of time to decay when unloaded or lightly
loaded. Wait a minimum of 60 seconds after stopping a
run before touching the power supply leads.
This equipment has a protective ground leakage current
of less than 0.5 mA using test methods defined in
CSA1010 and CSA151.
This equipment is for indoor use only.
4
Safety Information
Warning
Warnings alert you to a possibility of
personal injury.
Caution
Cautions alert you to a possibility of
damage to the equipment.
Note
Notes alert you to pertinent facts and
conditions.
Hot Surface
Hot surfaces alert you to a possibility of
personal injury if you come in contact
with a surface during use or for a period
of time after use.
Alert Signals

5
AC Input Power
90-132VAC, 50-60Hz, 100VA Max
Environmental
Operating temperature: 0-40°C, 0-95% R.H. non-condensing
Altitude: 2000m
Overvoltage category: II, IEC664
Pollution degree: 2, IEC664
DC Output Power
10-300VDC, 75W Max
4-400mA, 75W Max
Ripple: ± 1%
Drift: ± 1%, after 30-minute warm-up
Specifications

When unpacking your FB300 power supply, be sure you
have received the following items.
•FB300 unit
•AC line cord
•This manual
Inspect your equipment and packaging material for signs
of damage. Damage to the shipping container may indi-
cate rough handling which could cause internal damage
to the power supply. If you suspect shipping damage to
the power supply, contact your carrier for instructions on
filing a claim. If you are missing any of the above items,
contact your supplier for instructions.
6
Unpacking and Installation

Location
Select a location that allows for 3" clearance behind the
power supply, and comfortable reach of the front panel
controls and cell connections. Do not block the vented
area of the case - on the front bottom of the unit, or the
fan area at the rear. Connect the electrophoresis appara-
tus to the power supply, making sure to match the red
positive lead to the red positive jack, and the black nega-
tive lead to the black negative jack. Power the unit on by
connecting the AC power cord to the power entry on the
rear panel then plugging the 3 prong AC plug into a
power source. Connect the power supply to a 3-prong
grounded AC outlet, using the AC cord provided with the
unit only.
7
Operation
Figure 1. Front Panel Controls
Black negative jacks
3 digit LED display Up/down
arrows
Red positive jacks
Gray ON/OFF key
Select key (Set)
Constant key (K)
Run key

Using the Power Supply
Press the gray key on the front center of the unit to
enable the control logic. The 3-digit display will illuminate
and show the setpoint value of the last saved run setup.
The FB300 preserves the run settings each time you start
a run. One set of conditions is saved for each mode, con-
stant voltage or constant current. The setpoint value, limit
parameter, time duration, and power-fail restart setting is
saved. This allows convenient setup for repetitive runs.
To change the control mode, press the constant key. This
key toggles control from constant voltage to constant cur-
rent. Each time, the FB300 will display the last saved set-
point value for that particular mode.
After selecting a control mode, either constant voltage or
current, and setting the limit and timed run parameters if
desired (see Constant Voltage/Current Operations) press
the Run key to energize the power supply output. The
power supply output will ramp up to the appropriate set-
point, while not allowing the limit parameter to be exceed-
ed. If the limit parameter should be exceeded, then the
power supply will crossover control modes, making the
limit parameter the new control setpoint. The front panel
constant mode LED indicator will change to indicate that
a crossover has occurred.
To stop a run in progress, press the Run key or the gray
ON/OFF key. The display will change to "OFF" signifying
that the output is no longer energized. Pressing run again
resumes the run from the previous point (i.e. for a timed
run). Pressing any other key will return to setup mode in
either constant voltage or constant current mode as
appropriate.
During a run, the display will automatically cycle between
voltage, current, and time displays. Voltage and current is
displayed as actually measured by the FB300. When dis-
playing the controlled setpoint, i.e. voltage for constant
voltage control, the arrow keys can be used to modify the
setpoint. The limit parameter cannot be changed during a
run. The time parameter displays elapsed time for an
untimed run, and time left for a timed run.
8
OPERATION

Constant Voltage Operations
Press the constant key (labeled K) until the LED indicat-
ing "Volts" is illuminated. Each time the constant key is
pressed, the FB300 will display the last saved setpoint
value for that particular mode. Use the UP and DOWN
arrow keys to change the setpoint to the desired value
(10-300 volts). The FB300 will not allow setting a voltage
setpoint outside of the allowed range. Press and hold
either arrow key to quickly change the setpoint.
The limit parameter, in this case current, is normally set to
the power supply maximum value of 400 mA. To set a
lower limit value, press the Set key until the LED indicat-
ing "mAmps" is illuminated. Use the UP and DOWN
arrow keys to change the limit to the desired value (4-400
mA). The FB300 will not allow setting a current limit out-
side of the allowed range.
In addition, the FB300 will never exceed the maximum
power output specification of 75W. When starting a run,
the power supply calculates a maximum limit parameter,
above which the power supply maximum power output
rating would be exceeded. If this value is lower than the
limit setpoint entered, the FB300 will use this lower limit
parameter.
Constant Current Operations
Press the constant key (labeled K) until the LED indicat-
ing "Amps" is illuminated. Each time the constant key is
pressed, the FB300 will display the last saved setpoint
value for that particular mode. Use the UP and DOWN
arrow keys to change the setpoint to the desired value (4-
400 mA). The FB300 will not allow setting a current set-
point outside of the allowed range. Press and hold either
arrow key to quickly change the setpoint.
The limit parameter, in this case voltage, is normally set
to the power supply maximum value of 300 volts. To set a
lower limit value, press the Set key until the LED indicat-
ing "Volts" is illuminated. Use the UP and DOWN arrow
keys to change the limit to the desired value (10-300
volts). The FB300 will not allow setting a voltage limit out-
side of the allowed range.
9
OPERATION

In addition, the FB300 will never exceed the maximum
power output specification of 75W. When starting a run,
the power supply calculates a maximum limit parameter,
above which the power supply maximum power output
rating would be exceeded. If this value is lower than the
limit setpoint entered, the FB300 will use this lower limit
parameter.
Timed Operations
It is possible to enter an amount of time for the power
supply to provide power, after which it will automatically
shut off. Use this feature for timed runs.
Press the Set key until the LED indicating "Time" is illumi-
nated. Use the UP and DOWN arrow keys to change the
time duration to the desired value (0-999 minutes). A time
duration of zero effectively disables timed run mode.
When running a timed run, the time parameter displays
the time remaining in the run. When running a non-timed
run, the time parameter displays the elapsed time during
the run.
Automatic Power-Fail Restart
Automatic power-fail restart capability allows a timed run
which is interrupted by loss of AC power to be restarted
automatically, so that the total time programmed for the
time parameter will be met.
To enable power-fail restart, press and hold the Set key
when changing to the time parameter (from the current
parameter). While continuing to hold the Set key, simulta-
neously press and release the UP arrow key. The display
will show "PF" momentarily to indicate power-fail restart is
active. Whenever power-fail restart is enabled, "PF" is
displayed briefly as the time parameter is selected by
pressing the Set key. The power-fail restart feature can
be enabled either before or after changing the time dura-
tion to the desired value (0-999 minutes). Setting the time
duration to zero will automatically disable power-fail
restart (if you have enabled power-fail restart before set-
ting a time value, and want to disable it, then simply incre-
ment and then decrement the time parameter back to
zero to disable power-fail restart).
10
OPERATION
Note
Loss of AC power includes unplugging
the unit. When performing timed runs
with power-fail restart enabled, always
use the front panel controls to stop a
run in progress.