Fisher Scientific Midi300V/4 User manual

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Midi300V/4 Power Supply
Instruction manual
Midi300V/4
(FB79530 / 12613546)
Version 01a
Dec 2012

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Packing list
Midi300V/4
-1x Midi300V/4 Power Supply
-1x Power Cord
-1x Instruction Manual
Signed by:
Date:

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Warning
The MIDI300V/4 Power Supply has been thoroughly tested and found to
comply within the limits of CE regulation. It has been manufactured using the
latest technology and does not require maintenance. When used correctly
this unit poses no particular health risk, although it can deliver dangerous
voltage levels if used incorrectly. Accordingly, this power supply must only be
operated by fully qualified personnel adhering to the guidelines laid out within
this instruction manual. Although this power supply is equipped with all
necessary safety features against abuse and accidental failure, caution should
always be exercised when working with high voltage equipment. Any
individual intending to use this instrument should read the entire manual
thoroughly before operation.
1. Read the instruction manual thoroughly before use.
2. Never touch the power outlets with any conductive object (e.g. naked
metal wire) other than properly insulated power supply cables.
3. Do not spill liquid or insert metal objects inside the power supply.
4. Never block the ventilation holes or place the unit in any enclosure unless
there is adequate ventilation; never expose the power supply to a direct
heat source.
5. Never touch any part of the power supply assembly (i.e. power supply,
cables or electrophoresis tank) before switching OFF the power supply.
6. Never manipulate with wet hands.
7. Do not connect to ground any of the power outputs or the buffer within the
electrophoresis tank; the power outputs should be only connected to an
insulated electrophoresis tank equipped with a safety cover.
8. Do not connect any power supplies in series or in parallel.
9. Never open the back plate or remove the cover, otherwise an electric
shock may result. Repairs should only be made by the manufacturer or a
service technician authorised by the manufacturer.
10. Never use this power supply if the safety cover is not in position correctly.
11. Do not use the unit if there is any sign of damage to the external tank or

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cover. Contact the manufacturer or supplier immediately to replace or
repair any damaged parts.
12. Never use the power supply in the presence of flammable or combustible
material as fire or explosion may result.
13. Ensure that the power supply is only connected to an earthed power line.
Do not cut and splice the power line. When removing the power cord
from the wall, unplug it by holding the plug attachment and not by pulling
the cord. Do not hold the plug with wet hands or gloves.
Environmental Conditions
This power supply may only be installed and operated only under the following
environmental conditions:
1. For indoor use only
2. Relative humidity: ≤95%
3. Atmospheric pressure: 75 kPa –106 kPa
4. Altitude: ≤2000 metres
5. Operating temperature: ambient to 40°C
6. Pollution degree: 2
7. Mains supply voltage fluctuations up to ±10% of the normal voltage
Symbols
The symbols used on the power supply are explained below.
Indicates the potential for electric shock.
Consult the manual to avoid possible personal injury or instrument
damage.
Do not dispose of this power supply in municipal or domestic waste
once it has reached the end of its lifespan. Please recycle it if
possible.

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Table of Contents
Packing list .......................................................................................................... 2
Warning................................................................................................................ 3
Section 1 Introduction.................................................................................. 6
1.1 Overview ..................................................................................................... 6
1.2 Product description & Feature ..................................................................... 7
Section 2 Technical Specification................................................................ 8
Section 3 Installation Instructions............................................................... 9
Section 4 Operation Instructions................................................................. 9
4.1 Control interface.......................................................................................... 9
4.2 Start the operation..................................................................................... 10
4.3 To use the constant current or voltage mode................................................ 11
4.4 To adjust the time………………………………………………………………... 11
4.5 Starting and stopping the run……………………………………………………12
4.6 Pausing the run…………………………………………………………………...12
Section 5 Troubleshooting and Maintenance....................................................... 12
Section 6 Ordering Information.................................................................................. 17
Section 7 Warranty........................................................................................................... 17

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Section 1 Introduction
1.1 Overview
Front view
Rear view
Main power socket &
Fuse holder
ON/OFF Switch
Fan

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1.2 Product Description & Features
With nearly twice the current and power of the market leader’s equivalent unit,
at 700mA and 150W, the MIDI300V/4 offers a specification comparable to any
midi power supply presently available on the market. The MIDI300V/4 is
perfectly suited to use with all Fisher horizontal Sub-Gel systems and Verti-Gel
mini vertical gel units, and may also be adapted for specialist techniques
including the Comet Assay, and clinical and high throughput horizontal
electrophoresis. Microprocessor control with four sets of power terminals
allow simultaneous operation of as many electrophoresis units either at a
constant voltage or current setting, while the timer function may be set
continuously or up to a maximum 999 minutes when an alarm sounds to signify
termination of the run. A user-friendly interface houses a conspicuous 3-digit
LED to aid set up, as well as a convenient ‘pause / resume’ key, a particularly
useful feature during extended runs when it is necessary to access the gel tank
to monitor buffer levels and sample migration. Given its high specification
and remarkable versatility the MIDI300V/4 is relatively inexpensive, and
benefits from additional features such as a stackable design; rigorous in-built
safety mechansims; automatic crossover and dual voltage compatibility.

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Section 2 Technical Specification
Output range (programmable)
Voltage
2-300V
Current
1-700mA
Power
150W max.
Resolution
1V / 1mA
Type of Output
Constant voltage or constant current
Automatic crossover
Yes
Timer
1-999 min. with alarm; Continuous
Pause/resume function
Yes
Display
3-digit LED
Programmable Methods
-
Automatic recovery after power failure
Yes
Safety features
No-load detection; sudden load change
detection; overload detection; ground-leak
detection; over-voltage, current &
temperature protection;
maximum power output detection; shrouded
plugs and sockets
Operating conditions
Ambient-40C; ≤95% humidity
Stackable
Yes
Number of output jacks
4 sets in parallel
Regulatory conformity
EN-61010-1; CE
Dual voltage
Yes; 100-240 VAC
Construction
Flame retardant ABS-plate design with
aluminium base
Dimensions (WxDxH)
190x305x95mm
Weight
2.5Kg

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Section 3 Installation Instructions
The MIDI300V/4 Power Supply is a factory pre-installed instrument. The power
supply is ready for immediate use once it is unpacked and placed on a level,
dry bench surface that is adequately ventilated and away from sources of
direct heat.
Section 4 Operation Instructions
4.1 Control interface
There are seven buttons and four LED indicators on the faceplate.
The LED indicates the operation status of the unit.
a. Setup Mode (before pressing RUN/Start)
Each illuminated LED light shows the parameter currently selected on the
faceplate of the power supply. For example, if the voltage is selected the
VOLT LED will be illuminated.

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b. Operation Mode
Once the power supply is started, the LED will be illuminated on the
faceplate adjacent to the START/STOP button.
1. –Voltage Select
2. –Current Select
3. –Time Select
4. –Increase Select
5. –Decrease Select
6. –Pause Program
7. –Start/Stop Program
4.2 To start the operation
Note: To operate under constant voltage or constant current modes, adjust the
other parameter to the maximum value. For example, to operate under
constant voltage, adjust the current to the maximum output of 700mA before
running the power supply with the voltage set at the desired output setting.

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1. Place the power supply on a level, dry bench surface away from sources of
direct heat.
2. With the AC power switch in the OFF position, plug the power cord into a
grounded AC outlet of the appropriate voltage (i.e. 100V or 240V,
determined by the rating sticker near the AC cord on the back of the unit).
3. Switch on the power supply by pressing the ON/OFF button at the rear of
the power supply.
4. Connect the power supply cables from the electrophoresis unit by inserting
the red lead (+) into the red power supply output and the black lead (-) into
the black power supply output beneath the front fascia plate.
4.3 To use the constant voltage or current mode
* Press the or buttons to set a constant voltage or current
respectively.
5. Press the or buttons to adjust the voltage or current in 1V or
1mA increments or decrements to the required output value.
6. After adjusting your constant mode output value (e.g. voltage), set the other
parameter to the maximum output (e.g. current).
4.4 To adjust the time
7. After setting the desired current or voltage output value, press the
button, and then press key or key to adjust timer accordingly.

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For non-stop continuous operation, set the button to ‘zero’.
4.5 Starting and stopping the run
8. Press the key to start the run. Once the electrophoresis run starts,
the LED light will light up next to the button.
9. Press the key to terminate a timed run, and turn the AC power
OFF using the switch at the rear of the power supply.
10.The programmed settings will be retained within power supply memory and
displayed when the power supply is next switched on.
4.6 Pausing the run
11.Press key for temporary access to the gel. This interrupts the
power supply during the run, allowing access to the gel. A PAUSE
message will flash within the LCD screen, while the voltage and current will
read at zero value. The LED adjacent to the will also flash.
12.Press the key to resume electrophoresis. The voltage and

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current settings will be restored within the LCD screen after a few seconds.
Section 5 Troubleshooting and Maintenance
Many operating problems may be solved by reading and following the instructions in
this manual accordingly. Some suggestions for troubleshooting are given below. If
these suggestions fail to resolve the problem, contact the Fisher Scientific distributor
in your region for assistance. If troubleshooting service is required, please include a
full description of the problem.
Problem
Cause
Solution
No Display / lights
No AC power
Check if the power supply is unplugged,
or if the AC power source is a problem.
AC power cord is not
connected
Check AC power cable to ensure that it is
fits correctly within the power supply. Use
the correct power cable.
The fuse has blown
Replace the fuse.
Fuse repeatedly
broken
Hardware failure
Contact Fisher Scientific.
Operation stops
Electrophoresis cables
are not connected to
the power supply or to
the electrophoresis
unit(s). There is a
broken circuit in the
electrophoresis tank.
Check the connections to the power
supply and the electrophoresis tank to
ensure they are intact; check the
condition of wires in the electrophoresis
unit. Close the circuit by reconnecting
the cables. Press START/STOP to
resume the run.
High resistance due to
tape left on a pre-cast
gel; an incorrect buffer
concentration or
volume in the
electrophoresis tank
Ensure that any tape is removed from the
ends of a pre-cast gel, the buffers are
prepared correctly, and the recommended
volume of buffer is added to the
electrophoresis tank.

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High voltage
application set to run
on a very low current
The no load alarm on the display screen
needs to be disabled. Contact Fisher
Scientific to arrange return of power
supply for the appropriate circuit board
modification to be made. There may be
cost for this.
Err1
Error message;
power supply stops;
alarm sounds
Current exceeds the
maximum output for
the power supply
(>700mA)
Check if the buffer concentration or
molarity is appropriate. Excessive buffer
concentration or molarity may increase
conductivity. To clear the error message,
press the START/STOP button.
Err2
Error message;
power supply stops;
alarm sounds
Voltage exceeds the
maximum output for
the power supply
(>300V)
Press the START/STOP button to clear
the error message. Contact Fisher
Scientific if the problem persists.
Err3
Error message;
power supply stops;
alarm sounds
Ground leak detection
(1) Check the electrophoresis system for
earth leakage
(2) If the Err3 error message persists,
switch the power on/off and restart the
application.
Err6
Error message;
power supply stops;
alarm sounds
Power supply is
over-heating
(1) Turn off the power supply immediately
and check that there is sufficient
clearance around the power supply for
ventilation.
(2) If the Err6 error message persists and
the power supply cannot be restarted
disconnect it from the mains and contact
Fisher Scientific.
Err7
Error message;
power supply stops;
alarm sounds
Poor connection
between power cable
and power supply;
damaged power cable
or electrode
Check the power cables and electrodes
within the gel tank.

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A59
Power Supply stops; alarm
sounds with countdown to
zero: A58, A57 etc.
Power supply is restarting
Check the electrophoresis
conditions within the
system without
compromising personal
safety.
Encountering Problems
1. Check the troubleshooting section.
2. Call or email Technical Service.
3. If it is necessary to ship the unit back for repair, contact Fisher Scientific for a
Return Authorisation Number (RAN) and shipping instructions. The unit will
be repaired and returned to you as quickly as possible.
Replacing the Fuse
For additional fuses, please contact Fisher Scientific Ltd.
To replace the fuse:
1. Turn off the main power switch at the rear of power supply and detach the
power cord.
2. Open the fuse compartment located inside the Power Entry Module by
inserting a small flat-bladed screwdriver into the slot below the ON/OFF
switch at the rear of the power supply. Gently turn the screwdriver to pry
open the fuse compartment.
Note: The fuse compartment will not open with the power cord in place.
3. Pull the fuse holder out of the compartment and inspect the fuse. If the fuse
is burnt or there is a break in the fuse element, replace the fuse with an
identical type of fuse (T2AH250V; as shown in the figure below).

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4. Place the fuse holder back into the compartment.
5. Snap the cover closed.
Maintenance
The MIDI300V/4 Power Supply uses all solid-state components and should
require no maintenance or recalibration under normal use. If the unit must be
returned for repair, contact your local authorised Fisher Scientific distributor.

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Section 6 Ordering Information
Cat. No. Description
FB79530 Fisher Scientific MIDI300V/4, 300V, 700mA, 150W Power
Supply, 110-240 VAC

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Section 7 Warranty
Fisher Scientific Ltd. Power Supplies have a warranty against manufacturing
and material faults of twelve months from the date of customer receipt.
If any defects arise during this warranty period, Fisher Scientific will repair or
replace the defective parts free of charge.
This warranty does not cover defects resulting from accident or misuse, or
defects caused by improper operation.
Units where repair or modification has been performed by anyone other than
Fisher Scientific, or an appointed distributor or representative, are no longer
under warranty from the time the unit was modified.
Warranty is rendered invalid for those units which contain accessories or
repaired parts not supplied by Fisher Scientific or its associated distributors.
Fisher Scientific cannot repair or replace free of charge units where improper
solutions or chemicals have been used.
If a problem does occur then please contact your nearest Fisher Scientific
supplier.

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NOTES

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NOTES