Fisherbrand 12867913 User manual

Manual de funcionamiento
Dispensador de botella
Horizontal
Electrophoresis
Units
Instruction Manual - Gebrauchsanweis-
ung - Manuel d’utilisation - Manual de
funcionamiento
Bottle Top Dispenser - Flaschenaufsatz-Dispenser - Distributeur pour
flacons - Dispensador de botella
Bottle Top
Dispenser
12867913
2.5 mL
12877913
5.0 mL
12887913
10 mL
12897913
30 mL
12807923
50 mL
12817923
100 mL
Flaschenaufsatz-
Dispenser
Distributeur pour
flacons
Dispensador
de botella

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Bottle Top Dispenser 4
• Intended use of the instrument 5
• Specifications 5
• General safety instruction 5
• Diagram of the Bottle Top Dispenser 5
• Restriction of use 5
• Operating instructions 5
• Autoclaving 5
• Troubleshooting Guide 5
• List of recommended reagents 5
• Chemical Resistance Chart at 20°C 5
Intended use of the instrument 6
Specifications 6
General safety instruction 6
• A) Dispenser when not in use : 6
• B) Dispenser when in use : 6
Diagram of the Bottle Top Dispenser 7
Restriction of use 8
• Before using the Dispenser 8
• Assembly 8
Operating instructions 8
• Priming 8
• Dispensing 8
• Volume adjustment knob 9
• User calibration procedure 9
• Maintenance / Cleaning 9
• Dis-assembling the Dispenser for
Cleaning and Servicing 10
Autoclaving 13
• Dis-assembling for Autoclaving 13
• Re-assembling after autoclaving 13
Troubleshooting Guide 14
List of recommended reagents 15
Chemical Resistance Chart at 20°C 16
Flaschenaufsatz-Dispenser 20
• VERWENDUNGSZWECK 21
• SPEZIFIKATIONEN 21
• Diagram of the Bottle Top Dispenser 21
• Einsatzgrenzen 21
• Gebrauchsanleitung 21
• AUTOKLAVIEREN 21
• Fehlerbehebung 21
• List of recommended reagents 21
• Chemische Beständigkeit bei 20 °C 21
VERWENDUNGSZWECK 22
SPEZIFIKATIONEN 22
Allgemeine Sicherheitshinweise 22
• A) Wenn der Dispenser nicht in Gebrauch ist: 22
• B) Wenn der Dispenser in Gebrauch ist: 22
Diagram of the Bottle Top Dispenser 23
Einsatzgrenzen 24
• Vor der ersten Inbetriebnahme 24
• Montage 24
Gebrauchsanleitung 24
• Vorpumpen: 24
• Volumenregler 25
• Kalibrierung 25
• Wartung/Reinigung 25
• Demontage des Dispensers zur
Reinigung und Wartung: 26
AUTOKLAVIEREN 27
• Demontage für das Autoklavieren 27
• Montage nach dem Autoklavieren 28
Fehlerbehebung 29
List of recommended reagents 30
Chemische Beständigkeit bei 20 °C 31
Table of content

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Distributeur pour flacons 35
• Finalité d’utilisation de l’instrument 36
• Spécifications 36
• Instructions générales de sécurité 36
• Diagram of the Bottle Top Dispenser 36
• Restrictions d’utilisation 36
• Instructions d’utilisation 36
• Autoclavage 36
• Dépannage 36
• Liste des réactifs recommandés 36
• Tabla de resistencia química a 20 °C 36
Finalité d’utilisation de l’instrument 37
Spécifications 37
Instructions générales de sécurité 37
• A) Lorsque le distributeur n’est pas utilisé : 37
• B) Lorsque le distributeur est utilisé : 37
Diagram of the Bottle Top Dispenser 38
Restrictions d’utilisation 39
• Avant d’utiliser le distributeur 39
• Assemblage 39
Instructions d’utilisation 39
• Amorçage 39
• Distribution 39
• Molette de réglage du volume 40
• Procédure d’étalonnage par l’utilisateur 40
• Maintenance / Nettoyage 40
• Démontage du distributeur pour le nettoyage et la
maintenance 41
Autoclavage 44
• Démontage pour l’autoclavage 44
• Remontage après autoclavage 44
Dépannage 45
Liste des réactifs recommandés 46
Tableau de résistance chimique à 20 °C 47
Dispensador de botella 51
• Uso previsto del instrumento 52
• Especificaciones 52
• Instrucciones de seguridad generales 52
• Diagram of the Bottle Top Dispenser 52
• Limitaciones de uso 52
• Instrucciones de funcionamiento 52
• Esterilización por autoclave 52
• Solución de problemas 52
• Lista de reactivos recomendados 52
• Tabla de resistencia química a 20 °C 52
Uso previsto del instrumento 53
Especificaciones 53
Instrucciones de seguridad generales 53
• A) Dispensador, cuando no se está utilizando : 53
• B) Dispensador, cuando se está utilizando : 53
Diagram of the Bottle Top Dispenser 54
Limitaciones de uso 55
• Antes de usar el dispensador 55
• Montaje 55
Instrucciones de funcionamiento 55
• Cebado 55
• Dispensación 55
• Mando de ajuste del volumen 56
• Procedimiento de calibración por parte del usuario 56
• Mantenimiento / Limpieza 56
• Desmontaje del dispensador para trabajos
de limpieza y mantenimiento 57
Esterilización por autoclave 60
• Desmontaje para realizar la esterilización
por autoclave 60
• Montaje después de la esterilización por autoclave 60
Solución de problemas 61
Lista de reactivos recomendados 62
Tabla de resistencia química a 20 °C 63
Table of content

Horizontal
Electrophoresis
Units
Bottle Top Dispenser
Instruction Manual
Bottle Top
Dispenser
12867913
2.5 mL
12877913
5.0 mL
12887913
10 mL
12897913
30 mL
12807923
50 mL
12817923
100 mL

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Bottle Top Dispenser
Instruction Manual

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Intended use of the instrument
The Bottle Top Dispenser is a general purpose laboratory instrument intended for use in laboratories for dispensing reagents and
chemicals which are compatible (Please refer the compatability chart on page XX) with the instruments.
Specifications
Vol.range mL Increment mL Accuracy
±% ±mL
CV
±% ±mL
0.25-2.5 0.05 0.6 0.015 0.2 0.005
0.5-5 0.1 0.5 0.025 0.1 0.005
1-10 0.2 0.5 0.050 0.1 0.010
2.5-30 0.5 0.5 0.150 0.1 0.030
5-60 1.0 0.5 0.300 0.1 0.060
10-100 2.0 0.5 0.500 0.1 0.100
General safety instruction
A) Dispenser when not in use :
• Piston Barrel should always be empty
• Nozzle cap should be fixed on the nozzle
B) Dispenser when in use :
• Nozzle cap should be removed
• Place a receiving vessel under nozzle before starting the operation
• Never use force

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Diagram of the Bottle Top Dispenser
Bottle Top Dispenser
Instruction Manual

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Restriction of use
NEVER use the Dispenser with :
• Liquids which are not compatible with PTFE, FEP and Borosilate Glass
• Hydrofluoric acid
• Liquids which contain solid particles
• Temperature limits are 15°C to 40°C
Before using the Dispenser
Check that the instrument has not been damaged in transit
Assembly
The Dispenser is packed with the dispenze nozzle attached
and the inlet feed tube removed. The lenght of FEP inlet tubing
provided should be adjusted to fit the particular reservoir. Longer
lengths on inlet tube are available on request. The threaded
platform base of the Dispenser has a 30mm screw thread. The
assembled Dispenser is screwed to the reservoir using gentle
had torque applied to the threaded platform base only. Removal
should also be by means of hand torque applied to this same
base. Do not operate the piston until the unit is safely and fully
mounted on the reservoir bottle. Six adaptators are supplied to
suit containers with a 28mm, 30mm, 32mm, 36mm, 40mm and
45mm screw neck.
Operating instructions
Priming
Place a container under the Dispenser’s delivery nozzle. Remove the Nozzle Cap. Set the “Volume adjustment knob” to the maximum
volume to enable free movement of the piston.
Prime the unit with a few gentle up and down strokes, taking the piston right down to it’s lowest stop position and lifting it up.
Repeat until a steady bubble free flow is visible in the barrel.
Dispensing
• Ensure that the nozzle cap is removed.
• Ensure that a receiving container is placed the nozzle.
• Dispenser is now ready for dispensing.

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Volume adjustment knob
Volume adjustment knob is simple and
easy to use. It is screw type knob. Simply unscrew the knob and slide up or down to
set the desired volume by aligning the pointer on the volume adjustment knob with
the scale on the main housing.
Tighten the screw after aligning with the scale.
User calibration procedure
Dispenser has been laboratory calibrated to its nominal volume. However, due to changes in
environmental conditions and the viscosity of the media which you dispense, a re-calibration might be
required. You can either re-calibrate at regular intervals such as once a week or whenever you notice
that the dispensed volume is different from the volume displayed by the unit.
To fully re-calibrate your Dispenser follow the following steps:
1. Set the Dispenser to the nominal volume or any other volume which is the most common volume you
dispense.
2. Follow the common rules for calibration used in statistical quality control (ISO 8655/2). Set the volume
and Dispense five full volumes of distilled water at 20°C on Electronic Balance to establish the actual
mean volume of liquid dispensed. If the gravitational average result varies from the volume displayed,
you should re-calibrate the Dispenser.
3. For re-calibration pull the cap outwards to expose the Calibration Nut.
4. Using the calibration tool, turn the calibration nut clockwise to reduce the volume and anticlockwise
to increase the volume. Repeat this procedure a few times till the desired volume is achieved.
Maintenance / Cleaning
Note: All maintenance should be carried out wearing suitable eye protection and protective clothing. If
in doubt, consult your safety officer.
1. Make sure that the Dispenser is completely empty.
2. Place the instrument into an empty sink together with its reservoir.
3. Unscrew the threaded platform base from the reservoir and lift the dispenser’s intake tube carefully
out of the reservoir, whilst tapping it against the reservoir’s aperture to shake off any droplets from
Volume adjustment knob
Cap
Bottle Top Dispenser
Instruction Manual

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the intake tube.
4. Hold the dispense nozzle over the aperture of the reservoir and apply gentle piston strokes in order to return any syringe contents
into the reservoir.
5. Empty the instrument completely and flush thoroughly with distilled water.
6. If the piston barrel is still not completely clean, you need to dis-assemble the dispenser. Refer Dis-assembling procedure given
below.
Dis-assembling the Dispenser for Cleaning and Servicing
Procedure to dis-assemble the PISTON
• 1- Pull the cap outwards to expose the Calibration Nut.
• 2- Unscrew the Calibration Nut with the help of calibration tool to dis-assemble the Piston and shaft out of the main housing.
• 3- Unscrew the piston from the shaft
Procedure to dis-assemble the BARREL
• 1- Unscrew the Barrel Cap using the special tool provided with the instrument.

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• 2- Remove the Barrel Cap.
• 3- Lift the upper housing to expose the Barrel and cover.
• 4- Unscrew Chuck Nut 1 and remove Barrel cover.
• 5- Glass Barrel in now exposed.
• 6- Gently pull the barrel upwards to detach itfrom the Valve Manifold.
• 7- Barrel has been dis-assembled for cleaning.
Bottle Top Dispenser
Instruction Manual

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Procedure to dis-assemble the DELIVERY and the VALVE MANIFOLD
• 1- Remove the delivery pipe cover by pulling it upwards from the slot.
• 2- Unscrew and remove the delivery pipe.
• 3- Unscrew the chuck Nut 2.
• 4- Remove Chuck Nut 2 and pull out the valve manifold

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Autoclaving
Dis-assembling for Autoclaving
• 1- Unscrew Chuck Nut 1
• 2- Pull Chuck Nut 1 along with barrel cover, upper housing and piston all the way up.
• 3- Autoclave the two sub-assemblies at 0 121 C and 15 psi pressure for 10-15 mins.
Re-assembling after autoclaving
• 1- Push the Piston in the Glass Barrel gently and go all the way down. (Caution : Ensure that the Nozzle cap is removed)
• 2- Tighten chuck nut 1 by screwing it properly.
• 3- Dispenser is now ready for use. No Re-calibation is required after Autoclaving.However, a quick calibration check is
recommended.
Bottle Top Dispenser
Instruction Manual

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Troubleshooting Guide
Most problems can be avoided by reading and following the instructions in this operating manual. Below we list some of those
most commonly experienced along with suggestions for solving them. If, however, these should not resolve the issue, or if you have
questions not covered below, please contact Fisher Scientific.
Trouble Possible cause Solution
Air bubbles appear in discharge nozzle • Liquid reservoir is empty • Refill reservoir and prime unit
• Too fast filling action • Fill and dispense more slowly
• Leaking piston • Clean piston. If problem persists
replace piston
• Leaking discharge valve • Clean unit by flushing throughly.
If problem persists, replace the
discharge valve
Barrel does not fill with liquid • Inlet tube not fitted correctly • Connect inlet tube correctly
Dispensing not possible • Blocked dispense nozzle • Disassemble the dispense nozzle
and flush with cleaning fluid
• Discharge valve stuck • Clean unit by immersing valve
assembly in cleaning fluid. If
problem persists, replace valve
assembly
Wrong dispense volume • Instrument not calibrated • Follow steps of User Calibration

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1,4-Dioxane
1-Butanol
Acetaldehyde
Acetic acid, 50%
Acetone
Acetonitrile
Acrylonitrile
Adipic acid
Allyl alcohol
Aluminium chloride
Amino acids
Ammonium chloride
Ammonium hydroxide, 30%
Amyl alcohol (Pentanol)
Aniline
Barium chloride
Benzaldehyde
Benzene (Benzol)
Benzine (Gasoline)
Benzyl alcohol
Biuret reagent
Boric acid, 10%
Calcium carbonate
Calcium chloride
Carbon tetrachloride
Chromic acid, 10%
Chromic acid, 50%
Chromosulfuric acid
Copper sulfate
Cresol
Dichlorobenzene
Dichloroethane
Lactic acid
Magnesium chloride
Mercury Chloride
Methanol
Methyl propyl ketone
Monochloroacetic acid
n-Amyl acetate
n-Butyl acetate
Nitric acid, 70%
Nitrobenzene
Octane
Oleic acid
Oxalic acid
Pentane (n-)
Perchloric acid, 10%
Phenol
Phosphoric acid, 85%
Potassium chloride
Potassium dichromate
Potassium hydroxide
Potassium permanganate
Propanol
Propylene glycol
Propylene Oxide
Salicylic acid
Silver acetate
Silver nitrate
Sodium acetate
Sodium dichromate
Sodium hydroxide, 30%
Sulphuric acid, 98%
Diethylene glycol
Dimethylformamide (DMF)
Ethanol
Ethy acetate
Ethylene glycol
Formaldehyde, 40%
Formic acid, 100%
Glycerol
Heating oil (Diesel oil)
Hexane
Hydrochloric acid, 37%
Iodine / potassium iodide solution
Isobutanol
Isopropanol (2-Propanol)
Isopropyl benzene
(Cumene)
Tartaric acid
Tetrachloroethylene
Tetrahydrofuran (THF)
Tetramethylamminium hydroxide
Toluene
Trichloroacetic acid
Trichloromethane (Chloroform)
Triethylene glycol
Turpentine
Urea
Xylene
Zinc chloride, 10%
Zinc sulfate, 10%
List of recommended reagents
These recommendations are carefully checked and correspond
to the current state of knowledge. If you need statements for
chemicals which are not given in the list, please do not hesitate
to contact us.
CAUTION
• Do not use HF or reagents not compatible with PTFE or Borosilicate Glass.
• If used with strong acids, it is advised to rinse & remove instrument at the end of every working day & store safely.
Bottle Top Dispenser
Instruction Manual

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Chemical Resistance Chart at 20°C
Liquids dispensed with the dispenser will be in contact, contantly, with the following materials; Borosillicate glass, (BSG), PTFE &
FEP. The following table is a guide to help with the queries regarding liquid compatibility.
Please note that these tables are just a guide. We recmmend taht if there is a question regarding liquid compatibility you should
exercise caution in use and refer to other chemical tables where available. Good laboratory practice would be to rinse out the liquid
handing unit at the end of each day with distilled water to prevent corrosive liquids being left in contact with the parts for too long.
CHEMICAL Acids BSG PTFE FEP
Acetic, Glaical R
Acetic, 25% RRR
Hydrochloric, Concentrated R
Hydrochoric, 25% RRR
Sulphuric, concentrated R
Sulphuric, 25% RRR
Nitric, Concentrated R
Nitric, 25% R
Phosphoric, 25% RRR
Formic, 25% RRR
Trichloroacetic 10% RRR
Formic Acid, 85% RRR
Arsenic Acid R
Boric Acid, 10% RRR
Chromic Acid, 20% RRR
Hydrofluoric Acid, 35% NR exceptions R
Phosphoric Acid 85% RRR
Nitric Acid, 50% RRR
Sulphuric Acid, 95% RRR
Alkalies
Ammonium Hydroxide, 25% RRR
Sodium Hydroxide RRR
Potassium Hydroxide RRR
Sodium Hydroxide RRR
Alcohols
Methanol, 98% R R
Ethanol, 98% R
Ethanol, 70% R

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CHEMICAL Acids BSG PTFE FEP
Isopropanol, n-Propanol R
Amyl Alcohol, Butanol R
Benzyl Alcohol RRR
Ethylene Glycol RRR
Propylene Glycol RRR
Glycerol RRR
Hydrocarbons
Hexane, Xylene RRR
Toluene, Benzene RRR
Kerosene, Gasoline R
Tetralin, Decalin R
Halogenated Hydrocarbons
Methyl Chloride R
Chloroform RRR
Trichloroethylene RRR
Monochlorobenzene, Freon R
Carbon Tetrachloride RRR
Ketones
Acetone RRR
Methyl Ethyl Ketone R R
Isopropylacetone R
Methyl Isobutyl Ketone R
Ethyl Acetate R R
Methyl Acetate R
Amyl & Propyl Acetate R
Butyl Acetate RRR
Propylene Glycol Acetate R
2-Ethoxyethyl Acetate R
Methyl Cellosolve Acetate R
I Benzoate R
Isopropyl Myristate R
Tricesyl Phosphate R
Bottle Top Dispenser
Instruction Manual

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CHEMICAL Acids BSG PTFE FEP
Oxides-Ethers
Ethyl Ether R
1,4 Dioxane & Tetrahydrofuarn RRR
Dimethylsuphoxide (DMSO) RRR
Isopropyl Ether R
Solvents with Nitrogen
Dimethyl Formamide RRR
Diethylacetamide R R
Triethanolamine R
Aniline RRR
Pyridine RRR
Miscellaneous
Phenol, Aqueous, 10% R
Formaldehyde Solution, 30% RRR
Hydrogen Peroxide, 30% RRR
Silicone Oil & Mineral Oil R
Pyridine RRR
Acealdehyde RRR
Ammonia, 25% aq. Sol R R
Ammonium R
Calcium Chloride aq. Sol RRR
Chlorine RRR
Chlorobenzene R
Fluorinated Hydrocarbones R
Hexane RRR
Iodine (tincture of) R R
Potassium Chloride aq. Sol R
Potassium Chloride aq. Sol R
Potassium Permanganate aq. Sol R
Magnesium Chloride aq. Sol R
Methylene Chloride RRR
Sodium Carbonate R
Sodium Dicromate RRR

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CHEMICAL Acids BSG PTFE FEP
Phenol, 100% RRR
Mercury RRR
Silver Nitrate RRR
Toluene RRR
Hydrogen Peroxide, 30% RRR
Xylene RRR
Zinc Chloride, 10% RRR
Zinc Sulphate, 10% RRR
Key
R= resistant VR = virtually resistant SR = slightly resistant NR = non-resistant
Exceptions = resistant with exceptions
Notes : depends on temperature - up to 300°C
Bottle Top Dispenser
Instruction Manual

Horizontal
Electrophoresis
Units
Flaschenaufsatz-Dispenser
Gebrauchsanweisung
Flaschenaufsatz-
Dispenser
12867913
2.5 mL
12877913
5.0 mL
12887913
10 mL
12897913
30 mL
12807923
50 mL
12817923
100 mL