GE HEALTHCARE Typhoon FLA 7000 User manual

GE Healthcare
Typhoon™ FLA 7000
User Manual

Table of Contents
51 Introduction ..........................................................................................................
51.1 Important user information .............................................................................................................
61.2 Regulatory information ......................................................................................................................
91.3 The Typhoon FLA 7000 laser scanner .........................................................................................
101.4 Typhoon FLA 7000 Control Software ...........................................................................................
152 Safety instructions ...............................................................................................
152.1 Introduction .............................................................................................................................................
152.2 General precautions ............................................................................................................................
162.3 Personal protection .............................................................................................................................
162.4 Laser safety .............................................................................................................................................
162.5 Radiation safety ....................................................................................................................................
172.6 Radiation hazard prevention ...........................................................................................................
182.7 Electric safety .........................................................................................................................................
192.8 Labels on Typhoon FLA 7000 ..........................................................................................................
232.9 Emergency procedures ......................................................................................................................
242.10 Recycling and disposal .......................................................................................................................
253 System configuration ..........................................................................................
253.1 Parts and functions ..............................................................................................................................
273.2 Instrument body ....................................................................................................................................
303.3 Accessories ..............................................................................................................................................
344 Installing and moving Typhoon FLA 7000 ........................................................
344.1 Site requirements ..................................................................................................................................
354.2 Transporting Typhoon FLA 7000 ...................................................................................................
354.3 Connections ............................................................................................................................................
375 Installing Typhoon FLA 7000 Control Software ..............................................
375.1 Installation sequence ..........................................................................................................................
375.2 Install Typhoon FLA 7000 Control Software (Windows XP) ...............................................
415.3 Install Typhoon FLA 7000 Control Software (Windows Vista) ..........................................
455.4 Install Typhoon FLA 7000 Control Software (Windows 7) ..................................................
536 Uninstalling and upgrading Typhoon FLA 7000 Control Software ..............
567 Registering, editing and deleting methods .....................................................
567.1 Registering a new method ...............................................................................................................
587.2 Editing a method ...................................................................................................................................
587.3 Deleting a method ................................................................................................................................
608 Filter module settings .........................................................................................
608.1 Registering a new filter name .........................................................................................................
608.2 Saving a filter combination ..............................................................................................................
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Table of Contents

618.3 Loading a filter combination ...........................................................................................................
628.4 Installing and replacing filters ........................................................................................................
669 Operation ..............................................................................................................
669.1 Operation overview .............................................................................................................................
669.2 Preparations before starting Typhoon FLA 7000 ...................................................................
679.3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software .......................
689.4 Selecting the reading mode .............................................................................................................
699.5 Setting the reading conditions .......................................................................................................
699.6 Placing the object to be scanned on the stage ......................................................................
739.7 Placing the stage in Typhoon FLA 7000 .....................................................................................
749.8 Scanning the sample ..........................................................................................................................
759.9 Adjusting the display parameters .................................................................................................
769.10 Saving the image data using a different file name ...............................................................
769.11 Viewing the image file in the analysis application ................................................................
769.12 Turning off Typhoon FLA 7000 .......................................................................................................
7710 Reading conditions, display parameters, and other settings ......................
7710.1 Reading conditions explained ........................................................................................................
8110.2 Display parameters explained ........................................................................................................
8210.3 Other settings .........................................................................................................................................
8511 About storage phosphor screens ......................................................................
8511.1 Introduction .............................................................................................................................................
8511.2 Compatible storage phosphor screens ......................................................................................
8511.3 Handling precautions .........................................................................................................................
8711.4 Preparing the storage phosphor screen ....................................................................................
8811.5 Exposing the storage phosphor screen .....................................................................................
9012 Maintenance .........................................................................................................
9213 Troubleshooting ...................................................................................................
97A Default sample detection methods ..................................................................
99B Specifications ........................................................................................................
4 Typhoon FLA 7000 User Manual 28-9607-62 AC
Table of Contents

1 Introduction
In this chapter
This chapter contains important user information, and a general description of Typhoon
FLA 7000 and its intended use.
1.1 Important user information
Read this before using the
Typhoon FLA 7000
All users must read this entire manual to fully understand the safe use of Typhoon FLA
7000.
Intended use
Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging,
ECL Plus Westerns, visible fluorescence and gel documentation.
Typhoon FLA 7000 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the
safe use of Typhoon FLA 7000. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
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1 Introduction

Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A Note is used to indicate information that is important for trouble-free
and optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your
procedures.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu. Hardware items are
identified in the text by bold text (e.g., Power switch).
1.2 Regulatory information
This section lists the directives and standards that are fulfilled by the Typhoon FLA 7000
system.
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1 Introduction
1.1 Important user information

Manufacturing information
ContentRequirements
GE Healthcare Bio-Sciences AB, Björk-
gatan 30, SE 751 84 Uppsala
Name and address of manufacturer
Sweden
INTERTEK SEMKO AB, NB 0413Name and ID of notified body
Uppsala, Sweden, Sept. 2009Place and date of declaration
See EC Declaration of ConformityIdentity of person authorized to sign
Declaration of Conformity
International standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
Safety requirements for electrical
equipment for measurement,
control, and laboratory use
EN 61010-1, IEC 61010-1, UL
61010-1, IEC 61010-2-81,
CAN/CSA-C22.2 No. 61010-
1
Harmonized with
2004/108/EC
EMC emissions and immunity
requirements for electrical
equipment for measurement,
control and laboratory use
EN 61326-1
VCCI Class A
FCC Part 15 B Class A
ICES-003 Class A
Harmonized with
2006/42/EC
Safety of machinery - Basic con-
cepts, general principles for de-
sign
EN-ISO 12100-1, 12100-2
Harmonized with
2006/42/EC
Safety of machinery - Principles
of risk assessment
EN-ISO 14121-1, 14121-2
Harmonized with
2006/95/EC
Safety of laser productsEN 60825-1, IEC 60825-1
Safety of laser productsUSA 21 CFR, Chapter I, Sub-
chapter J, Part 1040.10
Laser Products
Typhoon FLA 7000 User Manual 28-9607-62 AC 7
1 Introduction
1.2 Regulatory information

CE Conformity
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards. A copy of the Declaration of Conformity is available
on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Low Voltage Directive (LVD)2006/95/EC
ElectroMagnetic Compatibility (EMC) Directive2004/108/EC
CE Marking
The CE marking and the corresponding Declaration of Conformity is valid for the
instrument when it is:
•used as a stand-alone unit, or
•connected to other CE marked GE Healthcare instruments, or
•connected to other products recommended or described in the user documentation,
and
•used in the same state as it was delivered from GE Healthcare, except for alterations
described in the user documentation.
Note: This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of the FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be
required to correct the interference at his own expense.
This Class A digital apparatus complies with Canadian ICES-003. Cet
appareil numerique de la class A est conforme a la norme NMB-003
du Canada.
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1 Introduction
1.2 Regulatory information

Note: This is a class A product. In a domestic environment this product may
cause radio interference in which case the user may be required to take
adequate measures.
Laser standards
This instrument is a Class 1 (IEC60825-1:2007/EN60825-1:2007) laser product.
The following lasers can be installed in Typhoon FLA 7000:
Maximum powerWavelengthLaser and class
20 mW (CW)473 nmLD laser, class 3B
10 mW (CW)532 nmSHG laser, class 3B
45 mW (CW)635 nmLD laser, class 3B
80 mW (CW)650 nmLD laser, class 3B
Note: The beam divergence of all laser modules is collimated.
Regulatory compliance of
connected equipment
Any equipment connected to the Typhoon FLA 7000 should meet the safety requirements
of EN 61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected
equipment must be CE marked.
1.3 The Typhoon FLA 7000 laser scanner
The compact Typhoon FLA 7000 is an image analyzer that offers high performance,
multifunctionality, and high-speed reading. Benefits include:
Typhoon FLA 7000 User Manual 28-9607-62 AC 9
1 Introduction
1.2 Regulatory information

•Detection of high-sensitivity autoradiographs using storage phosphor screens.
•Four lasers and multiple filters enable the use of a variety of fluorescent dyes and
detection methods.
•Data is aquired through high resolution, sensitivity and wide range linearity.
•Compatibility with various types of samples, for example fluorescent labels,
fluorescent dyes, CBB stain, silver stain, X-ray film, and storage phosphor screens.
1.4 Typhoon FLA 7000 Control Software
System requirements
Minimum requirementParameter
Microsoft™ Windows™ XP Professional SP3 (32-bit)Operating system
or
Microsoft Windows Vista Business SP2 (32-bit)
or
Microsoft Windows 7 Professional (32-bit)
1 GBInternal memory
Intel Core 2 Duo processorProcessor
80 GBHard drive
1280 × 1024 pixelsMonitor resolution
One USB 2.0 portOther requirements
DVD-ROM drive
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1 Introduction
1.3 The Typhoon FLA 7000 laser scanner

Overview of the main window
The Typhoon FLA 7000 Control Software is used to control, use and supervise the Typhoon
FLA 7000.
1
2
3
4
5
6 7 8
FunctionPart
Phosphorimaging button: click to read a storage phosphor screen1
Fluorescence button: click to read a fluorescent sample2
Digitization button: click to perform digitization3
Method button: click to register or erase a combination of laser and filter.4
Filter Module button: click to change or register a filter.5
Preferences button: click to set the image file format, scan mode, correction
mode and to enable or disable the ND filter.
6
Filter: displays the loaded filters.7
Laser: displays the status of the loaded laser units8
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1 Introduction
1.4 Typhoon FLA 7000 Control Software

Overview of the reader settings
window
1
2
3
4
7
6
12
13
10
11
8
5
9
14 15
DescriptionPart
Image folder: specify where to save the file after the reading.1
File Name: enter the name of a file to save image data.2
Comment: enter an optional comment. The comment is embedded
in the file where the image is saved, and can be viewed with the
analyzing software.
3
Method: set the method to use in the scan. Up to 4 scans can be
performed in a row, all with individual methods.
4
PMT: set the voltage of the photo-multiplier tube. The higher the
value, the higher the sensitivity.
5
Click to increase the number of
scans
Click to decrease the number of
scans
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1 Introduction
1.4 Typhoon FLA 7000 Control Software

DescriptionPart
Set the scanning area.6
1Select a stage in the drop-down menu.
2Drag the red square to the desired position of the scanning
area.
3Drag the sides of the red square as needed to adjust the size
of the scanning area.
Pixel Size: set the pixel size.7
Choose a small pixel size for high quality images. Note that a small
pixel size setting increases the reading time and the size of the
image file.
Latitude: Specify the dynamic range.8
•L5 provides a larger detectable range than L4.
•L4 provides a finer density gradation, if the signals of the sample
areas are in the correct range.
Mode: Specify the mode for the reading area borders.9
•Select Grid to limit the borders to the grid lines.
•Select Free for an arbitrary area.
•Select All to use the entire Fluor stage as the reading area.
Save Condition...: click this button to save the current reading
conditions in a file, if desired. For details, refer to the User Manual.
10
Load Condition...: click this button to load previously saved reading
conditions, if desired. For details, refer to the User Manual.
11
File Size: the estimated size of the result data file is presented.12
Reading Time: the estimated time required for the scan is present-
ed.
13
Top: return to the main window.14
Start Scan: start the scan. The sample must be loaded before
starting a scan.
15
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1 Introduction
1.4 Typhoon FLA 7000 Control Software

System requirements
Minimum requirementParameter
Microsoft™ Windows™ XP Professional SP3 (32-bit)Operating system
or
Microsoft Windows Vista Business SP2 (32-bit)
or
Microsoft Windows 7 Professional (32-bit)
1 GBInternal memory
Intel Core 2 Duo processorProcessor
80 GBHard drive
1280 × 1024 pixelsMonitor resolution
One USB 2.0 portOther requirements
DVD-ROM drive
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1 Introduction
1.4 Typhoon FLA 7000 Control Software

2 Safety instructions
2.1 Introduction
The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that
may be hazardous. Before installing, operating or maintaining the equipment, you must
be aware of the hazards described in the user documentation. Follow the instructions
provided to avoid personal injury or damage to the equipment.
2.2 General precautions
WARNING
Do not use the equipment if smoke, strange noises or strange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.
Stop using immediately, turn off the power switch and unplug the
equipment from the power outlet. Contact your local GE Healthcare
representative to request repair.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
Healthcare representative for replacements.
WARNING
Do not place the equipment on unstable tables or on inclined
surfaces, as the equipment could be dropped or fall, resulting in
injury.
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2 Safety instructions

2.3 Personal protection
CAUTION
Always wear gloves, protective glasses and a lab coat or similar
when handling samples.
CAUTION
Always wear cotton gloves when handling storage phosphor
screens.
2.4 Laser safety
WARNING
Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.
CAUTION
Using procedures or adjustments other than those specified in this
manual may result in hazardous exposure to laser radiation.
2.5 Radiation safety
Radiation safety
This instrument is not equipped with any radioisotope (RI) or radiation generating unit,
and is therefore not regulated by radiation hazard prevention laws. However, the
instrument is capable of reading Storage phosphor screens which may be polluted by
radioisotopes (RI).
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument
immediately and follow the instructions of your radiation
administrator.
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2 Safety instructions
2.3 Personal protection

2.6 Radiation hazard prevention
Controlled area
Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards
due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56) defines the
controlled area as "a place where the dose equivalent related to external radiation
exceeds the dose equivalent determined by the Director General of the Science and
Technology Agency (hereinafter referred to as the Director General), the concentration
of radioisotope in the air exceeds the concentration determined by the Director General,
or the radioisotope density on the surface polluted by radioisotope exceeds the density
determined by the Director General."
Limit of superficial pollution
Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that
determines the quantity, etc. of radiating isotope specifies that the density of radioisotope
on the surface polluted by radioisotope must be one tenth of the density defined in Article
8.
Article 8 and Table 3 of this Notice define the limits as shown below:
1Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm2
2Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm2
Installation site of instrument
This instrument is capable of reading not only Storage phosphor screens but also
fluorescent pigment label samples (non-RI method). Therefore, it is recommended that
the user should install it outside the controlled area and use RI-indicated samples without
contacting them with Storage phosphor screens directly.
However, as described above, the Storage phosphor screen surface may be polluted by
radioisotope (RI), depending on the sample condition, since the instrument sticks the
sample to the 3H-compatible Storage phosphor screen surface and exposes it in an
auto-radiography experiment of the 3H label sample.
When a sample is in direct contact with a Storage phosphor screen, it is generally known
that the sample for making an auto-radiogram contains a relatively small quantity of
radioisotope. However, the degree of superficial pollution of the Storage phosphor screen
is greatly influenced by the dryness of the sample and dose of radioisotope in an
experiment and may exceed the limits mentioned in Limit of superficial pollution , on
page 17.
When the instrument reads a Storage phosphor screen with a polluted non-exposure
area, it may be polluted. The degree of such superficial pollution greatly differs with
users' operation conditions. Superficial pollution may exceed the limit mentioned above.
Note: As mentioned above, install this instrument in the RI controlled area if
the user uses RI-indicated samples that will be in direct contact with
Storage phosphor screens.
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2 Safety instructions
2.6 Radiation hazard prevention

Removal from the controlled area
If it is necessary to move the instrument and its laboratory, which were installed and
have been used in the controlled area, from the controlled area, make sure that the
degree of the superficial pollution is below the limits mentioned in Limit of superficial
pollution above.
2.7 Electric safety
WARNING
Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.
WARNING
Do not use the equipment within or near a sink, or in humid or dusty
environments. Fire and electric shock could result.
WARNING
Connect the power supply directly to a grounded wall power outlet.
The use of extension cords or multiple loads on one electrical outlet
could result in fire and electric shock.
CAUTION
Do not use the same power supply as that of large equipment such
as an air conditioner or centrifuge. Malfunction could result.
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2 Safety instructions
2.6 Radiation hazard prevention

2.8 Labels on Typhoon FLA 7000
Typhoon FLA 7000 serial number
The Typhoon FLA 7000 serial number is located on a label on the back of the instrument.
123456
Symbols used in safety labels
MeaningLabel
Warning! Read the user documentation before using the system. Do not
open any covers or replace parts unless specifically stated in the user
documentation.
The system complies with the requirements for electromagnetic compliance
(EMC) in Australia and New Zealand.
The system complies with applicable European directives.
Typhoon FLA 7000 User Manual 28-9607-62 AC 19
2 Safety instructions
2.8 Labels on Typhoon FLA 7000

Labels concerning use of
hazardous substances
MeaningLabel
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufac-
turer for information concerning the decommissioning of equipment.
Labels concerning laser light
MeaningLabel
CAUTION! Avoid exposure to the laser
beam when the lid is open.
CAUTION! Class 3B Laser product when
open and interlock defeated. Avoid expo-
sure to the beam.
Do not attempt to defeat the safety inter-
locks under the sample lid or behind the
filter door, or otherwise try to gain access
to the interior of the instrument through
any other opening. Exposure to laser light
can cause injury. Viewing the laser light
directly can cause blindness.
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2 Safety instructions
2.8 Labels on Typhoon FLA 7000