Genfine Ipipette User manual

GENFINE BIOTECH (CHANGZHOU)CO., LTD
http://www.genfine.com/
18501955112
[email protected]
Instructions For Electronic Pipette
Ipipette

Ⅰ
A- Pipetting key/
Confirmation key
B- Adjustment wheel
C- Tip ejection key
D- Function key
E- Speed key
F- Handgrip
A:
B:
C:
D:
F:
G:
E:
K: L:
H:
I:
J:
G- Label window
H- Pipette shaft
I- Tip cone
J- LCD display
K- Finger rest
L- External lithium battery
Instructions For Electronic Pipette
Ipipette

M :: N
M - ingUSB charg jack N - Power key
1000
1:
2:
:3
:4
1 - e indicatorBattery charg
2 g- Volume settin 3 -
Navigation bar
4 - Speed indicator
Ⅱ
H
Instructions For Electronic Pipette
Ipipette

HH
Suction
and Discharge
Key/Confirm Key/Wake Up Key
---In
the
main interface,
it
isresponsible forsuctionand discharge.
--When
it
isnecessary
toconfirm,
it
is the
confirm key.
--In
standbymode, you can wake up the systemto start working.
Adjusting Knob
---The volume increases with clockwise rotation and decreases
with counterclockwise rotation.
---Switch from automatic suction mode to quick separation
mode.
Toggle key
---It can be used in automatic suction mode, manual suction
mode and multiple suction mode Mode and multiple liquid
separation mode can be switched freely.
---Fast liquid discharge key, in the mode of manual liquid transfer
and multiple suction,Press the switch key to stop suction and
switch to discharge.
Speed Adjustment key
---In
the state of suction and
discharge, responsiblefor changing
the suction and
discharge speed. speedThe key
is divided into
three levels: H high, M Medium and L low.
---In automatic
suction, quick liquid separation, manual suction,
multiple suction in multiple liquid separation mode, press
the
speed keyfor a long
time to
change
to direct
discharge Liquid.
Power Prompt
---Green is
full power.
---Red is low power state, need to charge in time.
Pipetting
speed
--- It
is
aspirated.
--- It
is
in
the discharge
state.
Piston is
ready for
aspiration Liquid is
aspirated Discharg
eliquid Dischar
ge
com pletely Piston
goes back
to zero
HH H
Ⅲ
Liquid Volume Navigation Bar
Instructions For Electronic Pipette
Ipipette

Contents
- Product viewOverefore Use- Instructions BtructionsOperating Ins
- ing Instructions- Other Operat and Elimination- Fault Analysis
- Maintenance
- Attention rmation- Warranty Info
1
2
4
10
12
13
16
16
Instructions For Electronic Pipette
Ipipette

1
1 viewProduct Over、
。
brand-new product developed by---This product is a
ology Co.Ltd Move the pipette andGenfine Biotechn tto the principle of air displacement. Ioperate accordingte It is an ideal tool to transfer liquidis safe and accura
efficiently. b can quickly set the pipetting---The flexible kno eelect functions,the 1.44-inch color largparameters and s lays all kinds of pipetting informationscreen clearly disp
eration A single adjustment knob canto simplify the op
quickly convert a ariety of pipetting combinations.v
---Single channel lectric pipette is available in 9 e
specifications.
，
pecification infor ttemation of single channel electric pipeS
Type
IP-03
IP-04
IP-08
IP-05
IP-06
IP-09
Volume
eRang
0.2-10μl
0.5-20μl
2-100μl
5-200μl
10-300μl
10-500μl
50-1000μl
0.1-5ml
1-10ml
Minimum
Range
0.2μl
0.5μl
2μl
5μl
10μl
10μl
50μl
100μl
1ml
Maximum
Range
11μl
20.5μl
120μl
220μl
320μl
550μl
1050μl
5.1ml
10.5ml
Increment
0.1μl
0.1μl
0.5μl
0.5μl
2μl
2μl
1μl
10μl
100μl
（1）
（1）
（1）
（1）
able
he packing box and check whether all
nt with the list,ensure that no damage
ng transportation.
Box contents
①Electric pipette
②Charger
③USB Charging C
④Lithium Battery
Please open t
items are consiste
or loss occurs duri
⑤Grease （1）
⑥Product Instructions（1）
⑦Product Test Report （1）
IP-01
IP-02
IP-07
Instructions For Electronic Pipette
Ipipette

2
2. Instructions Before Use
2.1 Battery
Matters Needing Attention:
①It is strongly recommended that the battery be fully
charged before using this product.
②Please use the original charger and battery
combination. If used other batteries may cause damage
to the pipette. The damage caused by this is not
included in the warranty.
③Please charge in the room.
④Do not place the battery with metal or objects with
metal surface in case of battery short circuit.
⑤Waste batteries should be disposed of in
accordance with local laws and regulations. Please
contact the domestic waste is often treated by
classification.
⑥Do not put the battery into the fire to prevent
explosion.
⑦If you do not use the pipette for a long time, please
take out the battery.
Battery Specification:
Lithium Battery Nominal Voltage: 3.7V
Rated Capacity: 800mAh
Charging Limit Voltage: 4.2V
Charging Time: only 2 hours for the matching charger
Charger specification:
Input:AC 100-240V, 50/60Hz, Max 0.15A
Output:USB 5V, 1A
Charge
Before connecting the charger with the power
socket, please confirm whether the power socket is
consistent with the input voltage of the charger. Then
connect the charging line to the charger, connect the
charging line to the charging socket on the top of the
pipette, and connect the charger plug to the AC socket.
Tips:
1. When power on, the battery icon flashes to indicate
charging; Flashing green means the battery is full. When
the battery is powered off, the screen is closed and no
icon is displayed.
2. It is normal that the battery is slightly heated during
charging.
3. Please unplug the battery as soon as it is full.
4. It is recommended to replace the battery every two
years.
Instructions For Electronic Pipette
Ipipette

3
Install The Battery
Instructions For Electronic Pipette
Ipipette

4
Power On
Automatic suction interface
H
Specific Operation:
2.2 Installation and Push Out of Suction Head
Installation of suction head:
Before installation, please select the matching
suction head. During the installation, the pipette should
be inserted into the suction head vertically, rotated left
and right for half a circle, and then tightened. It is not
recommended to install the pipette by striking the
suction head. If it is operated in this way for a long time,
the parts of the pipette will be loose due to the impact
and the pipette will be damaged.
Washing suction head:
Before the formal pipetting, after installing the
suction head, please suck and drain 2-3 times.The suction
head is fully wetted to reduce the error of liquid transfer.
Push out the suction head:
Align the waste container and press the sucker rod
to push out the sucker.
Note: do not remove any liquid before the pipette is
installed with suction head.
3
Operating Instructions
3.1 Power On
Turn the power on key to the left to ON.
Shut Down
The power on key moves to the right to 0FF.
Standby
If the pipette does not operate for 2 minutes, it will
stand by automatically. Press the suction key to return to
use.Pipette Mode
The pipette has four modes, including automatic
suction, manual suction, multiple suction and multiple
separation. The mode can be switched by pressing the
switch key.
3.2 pipetting
3.2.1Automatic suction
（PIPETTE）
Automatic suction set volume is equal to the actual
volume, commonly used in general suction operation,
with automatic blowing function, can effectively avoid
liquid residue
Instructions For Electronic Pipette
Ipipette

Turn the knob counterclockwise
Press the suction key
Press the drain key
Turn the knob to adjust
the liquid volume
Then press the discharge key to
return to the automatic suction
interface
Press the suction key to suck
5
Note:
1. In the process of suction, do not hold the suction
head against the bottom of the container, so as not to
affect the accuracy of suction.
2. After the suction head inhales the liquid, stop for
a while, and then move the suction head away from the
liquid level: take 1000 μ 1, and stay for about 1 second;
Move 1000 μ 1, stay for 3 seconds.
3. When removing the liquid with viscosity or density
greater than water, it is recommended to pre wash the
suction head with the liquid to be removed for 3-5 times
before formal suction.
4. Use a small volume pipette, such as 10μl, 200μl,
300μl, 1000μl. When aspirating, the tip should be
immersed 3mm below the liquid surface; for larger
volume, such as a 5ml pipette tip, it should be immersed
below the surface 5mm.
H
H
H
H
Instructions For Electronic Pipette
Ipipette

H
Turn the knob to adjust to 10μl X10 times
Automatic suction interface
Turn the knob to quick dispensing
6
Press the aspiration button
Press the aspiration button to
aspirate liquid
X
Press the drain button to drain
Return to the automatic
aspiration interface
Press the drain button 10
times to drain the required
liquid
H
H
X
H
H
3.2.2 Quick dispensing (STEPPER)
In this mode, the user can control the discharge
volume and the number of discharges. This pipetting
combination is based on the automatic aspiration mode
and is discharged in batches during discharging. If you
need to dispense 10 times, drain 10μl each time, you
can aspirate 110μl forward. When discharging, set the
fluid volume to 10μl each time. Discharge 10 times, and
the remaining 10μl will be used as the replenisher to
drain.Specific Operation:
Instructions For Electronic Pipette
Ipipette

Long press the empty button
for 2 seconds
Press the drain button to drain
the liquid and return to the
automatic liquid suction
interface
7
Note:Since thequickdispensing modeisbased onthe extension
of the automatic aspiration modeFunction, so when
the user is using the quick dispensing process, please reserve a
supplement liquid. For example: dispense 80μl, 4 times, please
adjust the automatic aspiration volume to90μl, reserve 10μl as
replenishment solution.
Tip: In the quick dispensing mode, the user can drain the liquid
directly by pressing the drain button for 2 seconds if the user
does not need to drain the liquid in batches during the draining
process.
3.2.3 Manual Aspiration (MANUAL)
In this mode, the user can control the suction and
discharge volume by himself. The liquid suction and
discharge operations are completed manually.
Specific Operation:
Press the switch key
Automatic suction interface
Press the switch key to the
manual suction interface
X
H
H
H
H
Instructions For Electronic Pipette
Ipipette

H
H
Press and hold the aspiration button
Empty liquid and return to
manual suction interface
Press the drain button to drain the liquid
Long press the empty
button for 2 seconds
3.2.4 Multiple aspiration (ASPIRATE)
In this mode, the user can set up by himself to draw
the solution of equal volume or unequal volume, and
then discharge it again.
Specificoperation:Forexample,draw50μlof different
liquids in equal volume, a total of 2 times, and
then discharge again.
Press the switch key 2 times
Automatic suction interface
Switch to the multiple aspiration
interface and turn the knob to
adjust to the target value
H
8
H
Press the aspiration button
until the target volume
Instructions For Electronic Pipette
Ipipette

Press the aspiration button
twice to aspirate 50μl each
time, a total of 100μl
Press the aspiration button
until the target volume
H
Press the drain button to drain the
liquid and return to the multiple
aspiration interface
Press the switch button to
change from suction to
discharge
Press the drain button to
drain again
Press the switch key
H
H
3.2.5 Multiple Dispensing (MULTI)
In this mode, the user sets the volume of each dispense, and
the system automatically calculates the number of dispenses.
When liquid is aspirated, the total volume (including the
replenisher calculated by the system) is sucked at one time, and
the liquid is discharged according to the set value during
discharging. The specific operation is as follows: Take a 2-100 µl
pipette as an example, set 10 µl each time to dispense 10 µl, a
total of 10 times, draw 110 µl during aspiration, of which 10 µl is
the replenisher, not included in the total number of dispensing
times ):
，
Press the switch key 3 times
Automatic suction interface
Press the switch key 3 times
H
X
H
9
Instructions For Electronic Pipette
Ipipette

Press the aspiration button
to aspirate the total volume
Press the aspiration
button to aspirate
the total volume
Finally, press the drain button
to drain the remaining liquid
from the tip and return to the
multiple dispensing interface.
X
H
X
H
X
H
3.2.6 Other operating instructions
This pipette adopts an open and friendly operating
system, through the combined use of basic pipetting
modes, it can be matched with a variety of pipetting
combinations. Here are some commonly used pipetting
combinations:
1. Mixed pipetting
This pipetting combination is in automatic liquid
suction, manual liquid suction, and multiple suction
modes. A fter liquid suction, press the switch button to
returntotheautomaticliquidsuctioninterface,long press
the suction and discharge button, the liquid will be
automatically sucked and discharged after the liquid is
discharged 5 times,Mix the liquid inside the tip with the
liquid outside the tip.
2. Reverse pipetting
Forviscousorvolatileliquids,drawmore supplemental
liquid when absorbing the liquid, discharge
the target liquid volume first, and then discharge the
remaining liquid. If you need to pipette 100μl, aspirate
110μl, drain 100μl for the first drain and 10μl for the
second drain. Specific steps are as follows:
Turn the knob to set the aspiration volume to 110μl,
press theaspirationbutton toaspirate, andturn theknob
tosetthe first discharge volume to 100μl, press
the drain button to discharge 100μl, and then press
the drain button to drain the remaining 10μl.
10
Instructions For Electronic Pipette
Ipipette

3. Unequal dispense
The liquid is sucked and discharged several times at a
time. For example, 10μl of the first drainage, 20μl of the
second drainage, 30μl of the third drainage, 40μl of the fourth
drainage, 110μl of liquid can be aspirated and discharged
successively, and the last 10μl is the replenishing solution.
The specific operation is as follows:
Turn the knob to set the total amount of aspiration to
110μl, press the aspiration button to aspirate, turn the knob
to set the first dispensing volume to 10μl, press the drain
button to drain 10μl, turn the knob to set the second time
dispensing volume is 20μl, press the drain button to drain
20μl, turn the knob to set the third dispensing volume to
30μl, press the drain button to drain 30μl, turn the knob to
set the fourth dispensing volume to 40μl, press Drain 40μl
with the liquid key, and then press the liquid key again to
empty the tip.
4. Dispense aliquots after manual sampling
It is suitable for the situation where the target liquid
volume is unknown, and the liquid suction volume can be
flexibly controlled, and then the liquid can be divided equally.
Such as the layered liquid of the centrifuge tube, the specific
operation is as follows:
In manual mode, press and hold the suction button to
aspirate liquid, press the switch button once to switch to the
discharge state after liquid suction, turn the knob to set the
single dispensing volume, press the discharge button to
discharge, and repeat the discharge until all the liquid is
discharged.
5. Unequal sampling
Unequal volume sampling is an operation performed in
multiple aspiration mode. The system defaults multiple
pipetting as equal volume aspiration. If unequal volume
aspiration is required, the volume of aspiration is changed
during aspiration. For example, draw different amounts of
sample A10μl, draw 15μl of sample B, draw 20μl of sample
C, draw 25μl of sample D, the specific operations are as
follows:
In the multiple aspiration mode, turn the knob to 10μl,
press the aspirate button to extract sample A, turn the knob
to 15μl, press the aspirate button to extract sample B, repeat
the above operation to extract sample C and sample D, and
press after aspirating 70μl Click the switch button to switch
to the discharge state, and press the discharge key once to
discharge to complete the pipetting.
11
Instructions For Electronic Pipette
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12
4 Fault analysis and elimination
Solution
Piston
abnorma l
4.1Troubleshooting
Phenomenon Cause
○ The piston is too tightly
○ The piston is not
lubrication
○ Solidification of the
grease of the piston
○ Foreign matter or
damage on the piston
surface
○ There are impurities
and particles between
the piston, O-ring and
the suction head sleeve
○ Damaged O-ring
-Reinstall the piston
-Grease the piston
-Remove old grease
and re-grease
-Clean the piston or
replace the piston
-Clean and lubricate
O-ring and ejector
sleeve
-Replace O-ring
Inaccurate
pipetting
volume or
leakage
○Improper installation
of suction head
○Mismatched tips
○ Impurity particles
between the suction
head and the nozzle
○The tip is damaged
○Pipetting too fast
○The tip leaves the
liquid level too fast
○Foreign matter or
damage on the piston
surface
○Impurities particles
between the piston,
O-ring and the nozzle
○Insufficient lubrication
of O-ring and piston
○Damaged O-ring
○Incorrect operation
-Reinstall the tip
-Replace with a suitable
nozzle
-Clean the connection
nozzle and replace with
a suitable nozzle
-Replace with a new tip
-Adjust the pipetting
speed, slow aspiration
and slow release
-Stay for a few seconds
after aspirating it from
the liquid surface
-Clean or replace the
piston
-Clean and lubricate the
O-ring and the nozzle
-Apply grease
-Replace O-ring
-Follow the instructions
Residual
liquid in
the tip
○The selected model
with replenisher
○Mismatched tips
○Improper installation
of suction head
○High suction tip
○The liquid is too
viscous
-Press the start button
to empty the tip
-Use the right tip
-Reinstall the tip
-Replace the low
adsorption tip
-Select the reverse
pipetting mode,
pre-wash the tips, and
slow down the speed
Noise during
operation
○Insufficient lubrication
of the piston
○There is a foreign body
on the piston
○O-ring loose
-Apply grease evenly
to the piston
-Clean the piston and
apply grease
-Install the O-ring
correctly
Weakened
suction
function or
unable to
aspirate liquid
○Battery consumption
○Low battery
-Replace with new
battery
-Charge the battery
Instructions For Electronic Pipette
Ipipette

13
Phenomenon Cause Solution
Inaccurate
dispensing
of special
liquids
○Improper calibration
○Pipette liquids that are
easily volatile or density
is significantly different
from water
-Recalibrate with the
problem liquid
-Pre-wash tips or
recalibrate
The tip falls
off or installs
difficultly
○Poor quality tips
○The tip has been
damaged
-Use high-quality tips
-Replace with a new
tip
If you have any other questions, please contact your local
agent or call the national technical consultation hotline:
18501955112.
4.2 Leak test
The leakage of the pipette will directly affect the results of the
sampling and lead to errors in the experimental results.Therefore,
pipettes should be regularly tested for leaks.
Method:Aftersucking theliquid, placeit vertically inthe air for
30 seconds, and observe whether the liquid level drops and
whether the liquid drips from the tip. If there is, it means that the
pipetteis leaking.For solutions,please referto the"Fault Analysis"
chapter.
Note : If you transfer volatile liquids (such as most organic
solvents), it may be a problem of saturated vapor pressure. You
can aspirate and place the body several times before pipetting.
5 Maintenance
5.1 Cleaning and disinfection
In order to ensure the accuracy and precision of the pipette,
regular maintenance should be carried out according to the
specific usage. Especially after pipetting corrosive solvents, the
pipette should be cleaned. Through simple cleaning and
maintenance, the service life of the pipette can be appropriately
extended.
Note: Before cleaning or disinfecting, be sure to remove the
battery.
①External cleaning
Method: According to the conditions of use, you can wipe
the surface of the pipette with a wet cotton cloth moistened
with 70% ethanol or 60% isopropanol to remove external dirt,
then wipe it with double distilled water, and then dry it. Before
starting work every day, check the pipette for dust or dirt,
especially the tip connection part.
②Internal cleaning
Cleaning steps:
1. Follow the steps to disassemble the parts that need to be
cleaned.
2. Withdraw the tip sleeve and the tip connection nozzle with
70% ethanol or
Wipe with a damp cotton cloth with 60% isopropyl alcohol, rinse
with double distilled water, and then dry.
3. Generally, O-rings and pistons do not need to be cleaned. If
there is any solid particles, please wipe off gently with a clean
dry cotton cloth.
4. Apply a thin layer of grease to O-rings and pistons.
5. Follow the steps to assemble the pipette.
Instructions For Electronic Pipette
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14
③Disinfection
The lower end of the electric pipette adopts corrosion-resistant and
ant materials, which can be sterilized underh-temperature resisthig igh pressure. After cleaning the dismantledh temperature and hhig 121er end, sterilize it atlow ℃, 1bar for 20 minutes. After
nfection, cool down at room temperature for at least 2 hoursdisi ent uses anti-agingore use. This instrumbef
It is made of materials
nd sterilized under ultraviolet light withoutcan be disinfected aand
assembly.dis ttery2 Replacing the ba5.
r is insufficient, or the battery is depleted,hen the battery poweW ot operate normally, please connect theusing the pipette to nca
charging cable to charge or replace with a new battery that is fully
g the battery, please pay attention to thearged. When replacinchsitive and negative directions of the battery. After replacing thepottery, please turn on the device and check whether the pipette isbarmally energized.no
3 Storage5.
se, hang the pipette vertically on theDaily storage: After uis liquid in the pipette tip, do not place thette rack. When therepipe
pipette horizontally or upside down to prevent the liquid from
ing back and corrodiflow ng the piston.
t is not used for a long time, please take outWhen the instrumen
the re the cleaned and disinfected pipette in abattery, and then sto
dry, mperature. Avoid placing it in anclean, and normal te
ronment with large temperature changes, high humidity, andenvie amounts of dust.larg
Instructions For Electronic Pipette
Ipipette

Handling
Liquid
15
Prevention
method Cleaning and maintenance
methods
Aqueous
solution
and
buffer
Disassemble the lower end of the
pipette, rinse the contaminated
parts with double distilled water,
dry it below 60°C or let it dry
naturally, and apply a small amount
of grease to the piston.
Inorganic
acid or base Use filter
tips
After pipetting, remove the lower
end of the pipette and place it in a
ventilated place. If necessary, use
double distilled water to rinse the
contaminated parts, dry them
below 60℃or let them dry
naturally, and apply a small amount
of grease to the piston.
Infectious
fluid
Use a filter
tip for
forward
pipetting
Remove the lower end of the
pipette, soak it in a laboratory
disinfectant for 30 minutes, then
wash it with double distilled water,
and autoclave the contaminated
parts at 121°C, 1 bar, and 20
minutes.
Cell culture Use filter
tips
Remove the lower end of the
pipette, soak it in a laboratory
disinfectant for 30 minutes, then
wash it with double distilled water,
and autoclave the contaminated
parts at 121°C, 1 bar, and 20
minutes.
Organic
solvents Quick
pipetting
After pipetting, remove the lower
end of the pipette and place it in a
ventilated place. If necessary, rinse
the contaminated parts with double
distilled water, dry them at 60°C or
let them dry naturally, and apply a
small amount of grease to the
piston.
Radioactive
solution
Use a filter
tip for
forward
pipetting
Nucleic acid
or protein
solution
Remove the lower end of the pipette,
soak the contaminated parts in a
composite solution or a special
cleaning solution for 30 minutes,
then rinse with double distilled
water, dry at 60°C or air dry, and
apply a small amount of lubrication
to the piston fat.
Use a filter
tip for
forward
pipetting
Nucleic acid: Boil in aminoacetic
acid or hydrochloric acid buffer
(pH2.0) for 10 minutes, rinse with
double distilled water, dry at 60℃
or air dry, and apply a small
amount of grease to the piston.
Protein: Remove the lower end of
the pipette, clean it with detergent,
rinse with double distilled water,
and dry at 60°C or air dry.
Instructions For Electronic Pipette
Ipipette