Genfine Purifier HTS User manual

GENFINE
Purifier HTS
User manual V1.0
GENFINE BIOTECH (CHANGZHOU) CO., LTD.

Contents
Chapter 1 Product Introduction....................................................................................................... 1
1.1 Product Name.................................................................................................................... 1
1.2 Model.................................................................................................................................1
1.3 Intended Use..................................................................................................................... 1
Chapter 2 Function Introduction..................................................................................................... 2
2.1 Main Components..............................................................................................................2
2.2 Working Principle..............................................................................................................3
2.3 USB Port............................................................................................................................3
2.4 Consumables......................................................................................................................3
Chapter 3 Installation.......................................................................................................................5
3.1 Deliver Check................................................................................................................... 5
3.2 Environment Requirement................................................................................................5
3.3 Precautions.........................................................................................................................5
3.4 Installation Settings.......................................................................................................... 6
Chapter 4 Instructions for Use.........................................................................................................7
4.1 Starting...............................................................................................................................7
4.2 Programs............................................................................................................................ 7
4.3 Shutting Down...............................................................................................................12
4.4 Emergencies....................................................................................................................12
Chapter 5 Care and Maintenance.................................................................................................. 13
5.1 Instrument Maintenance..................................................................................................13
5.2 Cleaning.......................................................................................................................... 13
5.3 Magnetic Rods Cleaning..................................................................................................13
5.4 Waste disposal................................................................................................................ 13
5.5 Decontamination Procedure........................................................................................... 13
5.6 Packaging for repair.........................................................................................................14
5.7 Service Contract.............................................................................................................. 14
5.8 Fill in the system log.......................................................................................................14
5.9 Disposal of the instrument.............................................................................................. 15
Chapter 6 Technical Parameters....................................................................................................16
6.1 General Parameters..........................................................................................................16
6.2 Performance parameter....................................................................................................17
6.3 Safety parameters.............................................................................................................17
6.4 Compliance...................................................................................................................... 17
6.5 Error code....................................................................................................................... 18
Chapter 7 Other information..........................................................................................................19
7.1 Production Date............................................................................................................... 19
7.2 Service life.......................................................................................................................19
7.3 Components List..............................................................................................................19
7.4 Interpretation of Graphics................................................................................................19
7.5 Manual revision date....................................................................................................... 19
7.6 Target customers..............................................................................................................19
7.7 More information............................................................................................................20
7.8 Safety and User Guide.................................................................................................... 20

Chapter 8 Manufacturer and Medical Device Information........................................................... 21
Chapter 9 Order Information......................................................................................................... 22
Appendix A: Certificate of Decontamination.............................................................................. 23
Appendix B: System Log.............................................................................................................. 24

Page 1
Chapter 1 Product Introduction
1.1 Product Name
Automatic Nucleic Acid Extractor
1.2 Model
Purifier HTS
1.3 Intended Use
Purifier HTS （Figure 1-1 ）adopts the magnetic beads separation technology to achieve the
collection, release, transfer and incubation of the magnetic beads through the movement of the
magnetic rods and the magnetic tip combs. And by this, it can complete the entire nucleic acid
extraction process.
The instrument can be applied for the extraction and purification of nucleic acid from different
clinic samples. Purifier HTS is especially used on research and tests by the personnel who have been
trained professionally. It is used for automatic transfer and processing of magnetic beads in deep well
plates. It is recommended that good laboratory practice (GLP) should be followed to ensure the
reliability of the analysis. Refer to Chapter 6: Technical Parameters.
图1-1. Purifier HTS

Page 2
Chapter 2 Function Introduction
2.1 Main Components
2.1.1 Front View
Figure 2-1 Purifier HTS Front view
2.1.2 Rear View
Figure 2-2 Purifier HTS Rear View
2.1.3 Inside View
Figure 2-3 Purifier HTS Inside View

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Purifier HTS (Figure 2-3), The magnetic tip combs matches the deep-well Plate. The magnetic
head consists of two vertically moving platforms. One is used for the magnetic tip rodsand the other is
used for grasping the magnetic tip comb. During running, the magnetic tip comb and the magnetic rods
move vertically to complete the mixing of reagents and the adsorption and release of magnetic beads.
There are 4 plate positions on the rotary table. Up to 4 pieces of 96 deep well plates can be
placed simultaneously. Prior to the start of program, the samples and reagents are sub packed into the
deep well plate according to the extraction scheme. In each step, the deep well plate remains stationary,
and the moving component is the magnetic rod with magnetic rod tip comb.
The housing must be closed during operation. It can protect the sample from environmental
contaminants.
2.2 Working Principle
Purifier HTS automatic nucleic acid extractor adopts magnetic beads separation technology (Figure 2-
4). In this method, a disposable magnetic rod tip comb is loaded on the magnetic rod, and then the magnetic
beads are transferred between the deep hole plates containing specific reagents through the separation of the
magnetic rod and the magnetic rod tip comb, so as to realize the collection, release, transfer and incubation
of the magnetic beads. Different from the traditional nucleic acid extractor, Purifier HTS contains two
heating plates and two standard plates. Only four plates can complete the complete steps of nucleic acid
extraction by magnetic bead method, so as to realize the high-quality extraction of nucleic acid in the
sample, which greatly shortens the extraction time.
Figure 2-4. Diagram of working principle of the instrument
2.3 USB Port
There is a USB port on the front panel for importing and exporting programs.
2.4 Consumables
See "Accessory and Consumables List" for details and ordering information about Purifier HTS's
consumables, such as deep well plates, magnetic rod tip comb, and HEPA filters.

Page 4

Page 5
Chapter 3 Installation
3.1 Deliver Check
3.1.1 Unboxing
Move the packaged instrument to the operation site. To prevent condensation of water vapor, the
instrument should be left in its protective, anti-static plastic packaging until the ambient temperature is
reached room temperature. Open Purifier HTS and accessories carefully. Remove the instrument from
the package and place it on a horizontal surface.
Caution Be careful not to touch or loosen screws or parts specially specified the in
the instruction manual. Otherwise, it may result in misplacement and
invalidate instrument warranty.
Warning Purifier HTS weighs about 27 kg, if transporting without package, please
handle carefully. It is recommended that two people move the equipment
together and take appropriate precautions to avoid injury.
Please keep the original packing materials for future transportation. The packing is designed to
ensure safe transportation and reduce damage in transit. The use of alternative packaging materials
may not achieve the effect. All instrument related documentation and accessories provided by the
manufacturer are retained for future use.
3.1.2 Integrity check
Check the integrity of the goods. Please check the complete documents and accessories with the
instrument according to the packing list. Visually check the shipping packages, instruments and
accessories for possible transportation damage. If any parts are broken, please contact the
manufacturer in time.
3.2 Environment Requirement
When installing Purifier HTS, avoid placing it in the places where there is a lot of dust, vibration,
strong magnetic field, direct sunlight or ultraviolet radiation, airflow, high humidity or high
temperature fluctuation.
Please place the instrument on a regular experiment table to ensure that:
• The working area is flat, dry, clean and secure, with extra space for accessories, cables, etc;
• At least 10 cm of free space is provided for ventilation on the table around the instrument;
• The ambient air is clean, free from corrosive steam, smoke and dust;
• Ambient temperatures range from 5°C (41°F) to 40°C (104°F);
• Humidity is low and condensation does not occur (relative humidity is between 10% and 80%);
• Install the Purifier HTS in a protected place where no one can step on or trip over the power
cord, and where it is easy to access the power cord when the plug needs to be removed;
• Care should be taken not to operate the instrument in an environment with potentially harmful
liquids or gases.
3.3 Precautions
• Always ensure that the laboratory power supply voltage meets the specification on the label on
the back of the instrument;
• Do not smoke or eat or drink while using this device;
• Clean hands thoroughly after handling the buffer solution;
• Follow regular laboratory procedures for handling samples that may be dangerous;• Adhere to
good laboratory practices and wear appropriate protective clothing such as disposable gloves and
laboratory coat;
• Ensure that the working area is well ventilated;
• Do not spill liquid inside or outside the device.

Page 6
Caution Do not place the instrument near magnetic tapes, computer discs or other
magnetic storage devices, such as credit cards, as they may be damaged by
the strong magnetic field of the instrument magnetic head.
Warning Do not put the Purifier HTS magnetic head near the computer monitor,
as this may damage the monitor.
The product has a strong permanent magnet. This product should not be
used by people wearing pacemakers or metal prostheses. Prosthetic
devices such as pacemakers or prostheses may be affected or damaged by
close contact with strong magnetic fields.
3.4 Installation Settings
This section describes the installation Setting that must be performed prior to operating or
relocating the instrument.
3.4.1 Power Connection
Warning Make sure that the power switch on the rear panel is in the
"O" position. Never connect an ungrounded power outlet. Do not use any
power cord other than the one provided by the manufacturer.
First connect the power cord to the power connector and insert into the instrument. Next, connect
the power supply to a properly installed, well-grounded power outlet.
3.4.2 Operation Check
First turn on the instrument to "I". Instruments are initialized for testing and adjusting. The
display interface is the user management interface. It is recommended that have a check, which use the
maintenance program to verify proper instrument operation. If the check is correct, you can continue
to run on your own.

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Chapter 4 Instructions for Use
4.1 Starting
Before turning on Purifier HTS, make sure that the voltage on the label which is on the left
bottom of the back panel matches the local voltage.
Warning Never operate the instrument on an ungrounded electrical outlet.
4.2 Programs
4.2.1 Home Screen
The default program displays a list of programs that are preset in the Purifier HTS software and
cannot be edited or deleted. The user program is the program that the user edits and saves
themselves, and the user can edit and delete the user program according to the experiment needs. New
program can edit new programs. New programs can also be edited. Display the current status
of the instrument，“Idel” means the instrument is idle condition，“Run” indicates that the instrument
is running a program，“Check” indicates that the instrument is in the state of power-on self-test.
4.2.2 Program Running
Click "User Program" to enter the program classification interface.