Genfine Purifier 32 User manual

GENFINE
Purifier 32
User Manual V2.2
GENFINE Biotech (Changzhou) Co., Ltd.

目录
Chapter 1 Product Introduction........................................................................................................1
1.1 Product Name........................................................................................................................1
Automatic nucleic acid extractor................................................................................................ 1
1.2 Model.....................................................................................................................................1
Purifier 32....................................................................................................................................1
1.3 Intended Use..........................................................................................................................1
1.4 Working Principle..................................................................................................................1
Chapter 2 Function Introduction...................................................................................................... 1
2.1 Main Components................................................................................................................. 1
2.2 Magnetic Bead Processing Principle.................................................................................... 3
Chapter 3 Installation and Packaging..................................................................................................5
3.1 Delivery Check......................................................................................................................5
3.2 Environment Requirement.................................................................................................... 5
3.3 Precautions and Restrictions................................................................................................. 5
3.4 Installation Settings...............................................................................................................6
3.5 Power Connection................................................................................................................. 8
3.6 Packaging Settings................................................................................................................ 8
Chapter 4 Instructions for Use....................................................................................................... 10
4.1 Starting................................................................................................................................ 10
4.2 Control Panel.......................................................................................................................10
4.3 Shutting Down.....................................................................................................................14
4.4 Emergencies........................................................................................................................ 14
Chapter 5 Care and Maintenance...................................................................................................15
5.1 Periodic and Preventative Maintenance..............................................................................15
5.2 Platform Cleaning............................................................................................................... 15
5.3 Magnetic Rods Cleaning.....................................................................................................15
5.4 Waste disposal.................................................................................................................... 16
5.5 Decontamination Procedure...............................................................................................16
5.6 Packaging for repair.............................................................................................................17
5.7 Service Contract.................................................................................................................. 17
5.8 Fill in the system log..........................................................................................................17
5.9 Disposal of the instrument................................................................................................. 17
Chapter 6 Technical Parameters.....................................................................................................19
6.1 General Parameters............................................................................................................. 19
6.2 Performance parameters......................................................................................................19
6.3 Safety parameters................................................................................................................ 20
6.4 Compliance..........................................................................................................................20
Chapter 7 Other information.......................................................................................................... 21
7.1 Production Date................................................................................................................... 21
7.2 Service life...........................................................................................................................21
7.3 Components List................................................................................................................. 21
7.4 Interpretation of Graphics....................................................................................................21
7.5 Manual revision date...........................................................................................................21

7.6 Target customers..................................................................................................................21
7.7 More information................................................................................................................ 21
7.8 Safety and User Guide........................................................................................................ 21
Chapter 8 Manufacturer and Medical Device Information............................................................23
Chapter 9 Order Information..........................................................................................................24
9.1 Purifier 32 configuration.....................................................................................................24
9.2 List of accessories and consumables.................................................................................. 24
9.3 List of kits............................................................................................................................24
Appendix A Certificate of Decontamination................................................................................. 26
Appendix B System Log................................................................................................................ 27

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Chapter 1 Product Introduction
1.1 Product Name
Automatic nucleic acid extractor
1.2 Model
Purifier 32
1.3 Intended Use
This instrument can be applied for the extraction and purification of nucleic acid from
different clinic samples. Purifier 32 is especially used on research and tests by the personnel who
have been trained professionally. It is recommended that good laboratory practice (GLP) should be
followed to ensure the reliability of the analysis. Refer to Chapter 6: Technical Parameters.
1.4 Working Principle
The principle is magnetic beads adsorption method. Purifier 32 (Figure 1-1) is designed for
automatic transfer and processing of magnetic beads in standard microwell plates. The patented
technology of the Purifier 32 system is based on the use of magnetic rods and microwell plates
with disposable and specially designed magnetic tip combs. The instrument may work without any
distribution or suction parts or devices. Samples and reagents, including magnetic beads, are
loaded into microwell plates according to corresponding instructions. The program selected by the
user through the touch screen has been uploaded to the instrument software. Purifier 32 software
can be used to create and run programs.
Figure 1-1. 32-Channel Automatic Extractor
Chapter 2 Function Introduction
2.1 Main Components
It usually consists of a mechanical part and an electrical part. This section shows the front
and back views of Purifier 32.
2.1.1 Front View
See Figure 2-1 for the front view of Purifier 32.

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Figure 2-1. Purifier 32 Front View
2.1.2 Rear View
See Figure 2-2 for the rear view of Purifier 32.
Figure 2-2. Purifier 32 Rear View
Purifier 32 (Figure 2-1) has 2 microwell plate positions. The magnetic tip combs match the
microwell plate. During each step, the microwell plate remains stationary and the only component
that moves is the magnetic head with magnetic tip comb and magnetic rod. The magnetic head
consists of two vertically moving platforms. One is used for the magnetic rods and the other is
used for magnetic tip comb. The magnetic tip comb with 32 channels can process 32 samples at
one time.
Prior to the start of program, samples and reagents are loaded into microwell plate. The
magnetic tip comb is placed on the correct microwell plate and can be automatically loaded onto
the moving platform. According to the program, the microwell plate is placed in the corresponding
plate position on the plate.
The sample chamber door must be closed during operation. It can protect the sample from
environmental contaminants.
The working principle of the instrument is MPP technology (Figure 2-4). Different from
other methods of magnetic bead purification for liquids transfer, this method transfers magnetic
beads between microwell plates containing specific reagents. The magnetic beds are transferred
with the help of magnetic rods and the magnetic rods are loaded with a specially designed

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disposable plastic magnetic tip comb.
2.2 Magnetic Bead Processing Principle
Figure 2-3. Magnetic Bead Processing Principle Sketch Map
2.2.1 Magnetic bead processing
Magnetic bead processing can be divided into four separate steps:
Bind magnetic beads
Release magnetic beads
Rinse magnetic beads
Concentration
2.2.2 Bind magnetic beads
In the magnetic bead binding process, the magnetic rod is completely inside the magnetic tip
comb. The magnetic rod and magnetic tip comb move slowly up and down in the microwell plate
to collect the magnetic beads to the top of the magnetic tip comb. After the magnetic rod and
magnetic tip comb are bound with the magnetic beads, they are lifted from the microwell plate and
transferred to the next microwell plate.
2.2.3 Release magnetic beads
After the magnetic beads are collected, the magnetic rod and magnetic tip comb are lifted
from the microwell plate together and transferred to the next microwell plate containing the
reagent. The magnetic rod and magnetic tip comb are placed together and the magnetic rod is
lifted.
The magnetic beads are released by moving the magnetic tip comb up and down at high
speed, and all the magnetic beads are fully mixed with the substrate of the next reaction.
2.2.4 Rinse magnetic beads
Magnetic bead cleaning is a common and important process that combines release and
collection in a microwell plate filled with cleaning solution.
In order to maximize cleaning efficiency, the magnetic rod and magnetic tip comb are
designed to have minimal liquid hanging performance.
2.2.5 Volume change in magnetic bead processing
The solution volume of the former microwell plate can be larger than that of the next
microwell plate to achieve the purpose of concentration (see Figure 2-4).

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Figure 2-4 Concentration Process in Magnetic Bead Processing
2.2.6 USB Port
There is a USB port on the front panel for updating the software of the touch screen and
motherboard.
2.2.7 Consumables
See "Accessory and Consumables List" for details and ordering information about Purifier
32's plastic consumables, such as deep-well plates, magnetic tip comb, HEPA filters and UV
lights.

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Chapter 3 Installation and Packaging
This chapter introduces the installation of Purifier 32.
3.1 Delivery Check
This section contains the procedures to be carried out upon receipt of the instrument.
3.1.1 Unboxing
Move the packaged instrument to the operation site. To prevent condensation of water vapor,
the instrument should be left in its protective, anti-static plastic packaging until the ambient
temperature is reached. Open Purifier 32 and accessories carefully, with the arrow on the transport
package upwards. Remove the instrument from the package and place it on a horizontal surface.
Warning Purifier 32 weights about 22.5kg. If transporting without package, please
handle carefully. It is recommended that two people move the equipment together and take
appropriate precautions to avoid injury.
When lifting the instrument, place the fingers on the bottom of both sides and keep the back
straight.
Please keep the original packing materials for future transportation. The packing is
designed to ensure safe transportation and reduce damage in transit. The use of alternative
packaging materials may not achieve the effect. All instrument related documentation provided by
the manufacturer is retained for future use.
3.1.2 Integrity and Damage Check
Check the packing list attached to the instrument according to the order. Visually check the
shipping packages, instruments and accessories for possible transportation damage. If any parts
are broken, please contact the manufacturer in time.
3.2 Environment Requirement
When installing Purifier 32, avoid placing it in the places where there is a lot of dust,
vibration, strong magnetic field, direct sunlight or ultraviolet radiation, airflow, high humidity or
high temperature fluctuation. Please place the instrument on a regular experiment table to ensure
that:
The working area is flat, dry, clean and secure, with extra space for accessories, cables,
reagent bottles, etc;
At least 10 cm of free space is provided for ventilation on the table around the instrument;
The ambient air is clean, free from corrosive steam, smoke and dust;
Ambient temperatures range from 10°C to 40°C;
Humidity is low and condensation does not occur (relative humidity is below 80%);
Caution should be taken not to operate the instrument in an environment with potentially
harmful liquids or gases.
3.3 Precautions and Restrictions
Always ensure that the laboratory power supply voltage meets the specification on the label

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on the back of the instrument (Figure 2-2);
Do not smoke or eat or drink while using this device;
Clean hands thoroughly after handling the buffer solution;
Follow regular laboratory procedures for handling samples that may be dangerous;
Adhere to good laboratory practices and wear appropriate protective clothing such as
disposable gloves and laboratory coat;
Ensure that the working area is well ventilated;
Do not spill liquid inside or outside the device.
Caution Do not place the instrument near magnetic tapes, computer discs or other
magnetic storage devices, such as credit cards, as they may be damaged by the strong magnetic
field of the instrument magnetic head.
Warning The product has a strong permanent magnet. This product should not be used
by people wearing pacemakers or metal prostheses. Prosthetic devices such as pacemakers or
prostheses may be affected or damaged by close contact with strong magnetic fields.
3.4 Installation Settings
3.4.1 Disassembly of set screw of magnetic rod holder
Before the main machine of the instrument is energized, it is necessary to use an Allen
wrench to remove the set screw of the magnetic rod holder. If the instrument is energized without
removing the set screw of the magnetic rod holder, the instrument will be damaged.
Figure 3-1. Installation Diagram of Set Screw of Magnetic Rod Holder

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Figure 3-2.Schematic Diagram After Removing the Set Screw of Magnetic Rod Holder
3.4.2 Installation of 96-well plate
The circular bottom of the 96-well plate with square hole and circular bottom just fits with
the concave clamping position of the heating tank; the heating tank is located under the 2/6 and
8/12 vertical row holes of the 96-well plate.
Figure 3-3. Installation of 96-well plate
3.4.3 Installation of magnetic tip comb
The magnetic tip comb must be inserted into the bottom of the card slot of the magnetic tip
comb to ensure that the magnetic rod is safely inserted into the magnetic tip comb during the
operation of the instrument.
Note: The reagent volume in the 96-well plate shall not exceed 1.2ml, otherwise the reagent
will overflow when the magnetic rod is shaken up and down.

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3-4. Effect Diagram after Installation of 96-Well Plate and Magnetic Tip Comb
3.5 Power Connection
Connect the power connector:
Warning Make sure that the power switch on the rear panel (Figure 2-2) is in the "O"
position. Never connect an ungrounded power outlet. Do not use any power cord other than the
one provided by the manufacturer.
1. Connect the power cord to the power connector and insert into the instrument (Figure 2-2).
2. Connect the power supply to a properly installed, well-grounded power outlet.
3.6 Packaging Settings
3.6.1 Machine Set-Up
Turn off the power and place the magnetic tip comb and deep-well plate into the instrument
to protect the magnetic rod. Next tighten with the set screw (Figure 3-1). If the set screw of the
magnetic rod holder is not tightened, the magnetic rod holder will move during transportation,
which will damage the instrument.
3.6.2 Instrument Packaging
Put the instrument in a bag, fix eight corners with foam and put into a carton. Then put the
carton into a big packaging box, and seal the packaging box for direct transportation.

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3-5. Inside schematic diagram of packaging box

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Chapter 4 Instructions for Use
4.1 Starting
Before turning on Purifier 32, make sure that the voltage on the label which is on the left
bottom of the back panel (Figure 2-2) matches the local voltage.
Warning Never operate the instrument on an ungrounded electrical outlet.
4.2 Control Panel
This section describes the control panel and internal software of Purifier 32.
4.2.1 Home Screen
The home page of the touch screen is shown below.
The default program displays a list of programs that are preset in the Purifier HT software
and cannot be edited or deleted. The user program is the program that the user edits and saves
themselves, and the user can edit and delete the user program according to the experiment needs.
New program can edit new programs. New programs can also be edited.
Display the current status of the instrument，“Empty” means the instrument is idle
condition，“Run” indicates that the instrument is running a program，“Check” indicates that
the instrument is in the state of power-on self-test.
4.2.2 Program Running
Click "User Program" to enter the program classification interface.

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Users can select any file in the table, for example by clicking the "DNA" button to enter
the list of programs. Click the program you want to run, and click "Select" to enter the program
details interface.
The program detail interface displays the step number, board position number, item name,
time, volume, temperature and other parameters. After confirming that the program is correct,
click "Run" to run the program.
During the program running, the interface displays the total time and remaining time of the
program. Click "Pause" to pause the program.
Click “Continue” to resume running.
Click "Cancel", a dialog box will indicate whether to exit the current running program or
not. Click "Cancel" to continue running, and click "Confirm" to stop running. Customers should
use this function with caution.

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4.2.3 New Program Creation
Click "New Program" on the main interface to create a new programs according to the
customer’s requirements. Enter the program name and select the program type. Click or
“Back” to return to the main screen. Click "Next" to enter the program editing interface.
Click "Add" to add steps after, click "Insert" to add steps before the selected step, and click
"Delete" to delete steps. Click "Edit" to set step parameters. Click "Back" to return to the main
screen.
Steps, parameters edition should be according to the actual situation to set the plate position,
solution volume and step options. The first step must be "Load", the last step must be "Unload".
The liquid volume should be set correctly. The instrument will identify the liquid level

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according to the volume filled in. Click , to return to the program details. After setting,
click "Next" to enter the parameter setting interface.
The heating temperature and time are selected according to the requirements of the
experiment. The temperature setting range is 0-99 °C, and the temperature can be switched.
The entry position of the deep-well plate is the position where the magnetic tip comb enter the
deep-well plates for mixing; 90% is recommended. High, medium and low vibration intensity
are available. The magnetic absorption position is the position where the magnetic rods enters
the deep-well plate for magnetic suction; 90% is recommended. There are two modes of
magnetic absorption: one-step magnetic absorption and step-by-step magnetic absorption. The
chosen magnetic absorption time is a cycle time, no less than 15 sec is recommended. Click
"Done" when you're ready.
After setting the program, click "Save", and the "Program Saved" dialog box appears; the
program will be saved in the folder corresponding to the user's program.
Click "Run" to run the program, and a dialog box will appear on the interface. Reconfirm
that the reagent plate and magnetic tip comb have been placed in the correct position, and click
"Confirm" to run the program. Click “Cancel” to return back to program details.
4.2.4 User Settings
The user can select "User Setting" on the main screen to set the parameters of Purifier HT.
After entering the user setting interface, the user can set the language, fan switch, light switch
and prompt tone switch. There are two modes of language: Chinese and English. "Factory
Setting" is not accessible to the users.

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4.2.5 UV Light Settings
Click "UV Sterilization" on the main interface to set UV disinfection, and set the time in the
time setting column. The remaining time is displayed as the time countdown. Click "Start" to start
UV disinfection. Click "Close" to stop UV disinfection.
4.3 Shutting Down
Turn off Purifier 32:
1. Press the power switch (Figure 2-2) on the rear panel of the instrument to the OFF position to
turn off Purifier 32. It is recommended to turn off the instrument at night and on weekends.
2. Wipe the surface of the platform and adjacent instrument with a soft cloth or paper towel with
distilled water, mild detergent (SDS, sodium lauryl sulfate) or soap solution.
3. If contaminated reagents have been spilled on the workbench, please clean them with 70%
ethanol or other disinfectants (see "Cleaning Procedures” on Page 16).
Warning Remove the deep-well plate and magnetic tip comb that are used in the
instrument. Dispose of all deep-well plates and magnetic tip comb as biohazard waste.
4.4 Emergencies
If an abnormal situation occurs during operation, such as liquid spilling into the instrument,
please follow the steps below:
1. Turn off the instrument (Figure 2-2).
2. Unplug the power plug immediately.
3. Implement appropriate corrective measures. But do not disassemble the instrument.
4. If the corrective measures taken do not help, please contact authorized technical service or your
local agent.

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Chapter 5 Care and Maintenance
5.1 Periodic and Preventative Maintenance
In order to ensure the reliability of daily operations, please clean up the dust in time to
prevent liquid leakage.
Do not use abrasive cleaners, as they may damage the paint surface.
It is recommended that you clean the surface of the instrument regularly to maintain its good
appearance. Wipe with a soft cloth dampened with warm water and neutral detergent.
If necessary, clean the outer surface of the instrument and the plate with clean low-pressure
compressed air, or a cloth dampened with water or a mild detergent.
Although Purifier 32 is made of high-quality materials, you must remove the spilled salt
solution, chemical solvent, acid or alkaline solution from the surface immediately to prevent
damage to the instrument.
Caution The painted surface can be cleaned with most laboratory cleaners. Dilute the
cleaner as recommended by theanol for a long time to avoid damage.
Clean the monitor with mild laboratory cleaners. The monitor has an easy-to-clean surface.
The plastic door and surface can be cleaned manufacturer. Do not expose the surface to
concentrated acid or ethwith mild laboratory cleaners or alcohol.
Warning If any surface is contaminated with biologically hazardous materials, it should
be cleaned immediately with a mild disinfectant solution.
5.2 Platform Cleaning
Keep the surface of the platform clean to prevent dust and dirt from entering the instrument.
At least once a week, clean the surface of the plate with a soft cloth or paper towel dampened with
mild detergent (SDS, sodium lauryl sulfate), soap solution or ethanol.If contaminated reagent is
spilled out on the plate, clean it with a cloth dampened with water, mild bleaching agent or mild
detergent.
5.3 Magnetic Rods Cleaning
If necessary, soak the magnetic rods in alcohol, mild detergent (SDS) or soap solution or
ethanol, and then wipe the magnetic rods finally.
Caution Do not place Purifier 32 near magnetic tapes, computer discs or other magnetic
storage devices, such as credit cards, as they may be damaged by the strong magnetic field of the
magnetic rod of Purifier 32.
Do not put the magnetic rod near the computer monitor, as this may damage the monitor.
Do not use metal tools to clean the magnetic rod.
Be careful not to break the magnetic rod when cleaning.

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Warning The product has a strong permanent magnet. This product should not be used
by people wearing pacemakers or metal prostheses. Prosthetic devices such as pacemakers or
prostheses may be affected or damaged by close contact with strong magnetic fields.
5.4 Waste disposal
Follow the specific regulations of laboratories and the country for handling biological
waste. For the disposal of contaminants, please refer to the relevant local regulations.
Warning The sample may be potentially contaminated. Dispose of disposable
deep-well plates, parafilm, magnetic tip comb, disposable gloves, syringes, and disposable
pipette tips as biohazardous waste.
5.5 Decontamination Procedure
If you have spilled out contaminant reagents, please perform a decontamination procedure.
Warning The decontamination procedure should be carried out by authorized trained
personnel in a well-ventilated room, wearing disposable gloves, protective glasses and clothing.
Follow regular laboratory procedures for decontamination. The decontamination
instructions provided with the reagents used should be followed. It is strongly recommended to
perform a complete decontamination procedure before transferring the instrument from one
laboratory to another, or before sending it to a repair service department.
5.5.1 Decontamination Reagent Types
70% ethanol
1%-3% VirkonTM solution
4% glutaraldehyde solution
Chloramine T
Microcde SQTM 1:64
4% DeconTM 90min
5.5.2 Decontamination Procedure
(1) Wear disposable gloves to protect yourself.
(2) Prepare the cleaning agent: 200ml 4% glutaraldehyde solution (or other
reagents recommended by the security officer).
(3) Empty the turntable.
(4) Turn off the power switch and disconnect the power cord.
(5) Use a cloth moistened with 70% ethanol to disinfect the outside of the
instrument.
(6) Put the instrument in a big plastic bag. Make sure the front door is open.
(7) Put a piece of cloth soaked in glutaraldehyde solution in the plastic bag. Make
sure that the cloth does not touch the instrument.
(8) Seal the instrument in a plastic bag for at least 24 hours.
(9) Take the instrument out of the plastic bag.

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(10) Clean the instrument with mild detergent.
(11) Use 70% ethanol to remove stains.
(12) After performing the decontamination procedure, attach a signed and dated
decontamination certificate on the transport package and attach it to the outside of
the package (see Appendix A: "Certificate of Decontamination").
5.6 Packaging for repair
If you need repair the instrument, Please package it following the guidelines below.
Caution Before removing the instrument from the laboratory or performing any repair on
it, it must be thoroughly cleaned. When sending the instrument for repair, please remember:
(1) Inform the reason for repair.
(2) Decontaminate the instrument before hand. Pack the instrument according to its state
before disassembly.
(3) Use the original packaging to ensure that the instrument will not be damaged during
transportation. Any damage will incur additional service charges.
(4) Return the instrument (or other items) with a signed and dated decontamination certificate
(see Appendix A: "Certificate of Decontamination"). And attach it to the outside of the package.
(5) After you contact the local agent or the manufacturer's technical service department, please
specify the disfunction.
(6) Please refer to “Chapter 6” for more information on storage and transportation
temperatures.
5.7 Service Contract
It is recommended that a service engineer trained by the manufacturer conduct a regular
maintenance and repair of the instrument every 12 months. This ensures that the product is
properly maintained and used without any problems.
5.8 Fill in the system log
System logs, including operation summary, maintenance procedures, error messages and
other useful information about system. These is very useful for proper maintenance of the system.
Please refer to Appendix B: "System Log". You can copy the form as many times as you need,
but keep the original blank form in the user manual.
5.9 Disposal of the instrument
If Purifier 32 must be discarded, please follow the specific regulations of the laboratory
and the country for the treatment of biological waste. The disposal of the instrument is
carried out in accordance with the laws and regulations of the local authorities on the
recycle of electronic equipment and waste. Disposal procedures vary from country to
country. For the original packaging and packaging materials recycle, please cooperate with a
recycler you are familiar with.
Warning Disinfect the instrument before disposing of it. Please refer to