Greiner Bio-One MULTI-MAGPEN 657896 User manual

INSTRUCTIONS
FOR USE
96 WELL MULTI-MAGPEN
Item No.: 657896
Your smart assistant for
3D cell culture transfer

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
Thank you for purchasing this Greiner Bio-One product. The Multi-MagPen is used to transfer, collect,
organize, or layer magnetic 3D cell cultures without disrupting their tissue architecture.
The Multi-MagPen uses a simple “pick-up-and-drop” method to reliably transfer 3D cell cultures between
microwell plates.
The magnets in this kit are strong, can damage electronics, and cause injury if not handled correctly.
Read the safety precautions below carefully to ensure correct handling of the magnets.
SAFETY PRECAUTIONS
To guarantee problem free and safe operation of the Multi-MagPen please read these safety precautions
before using.
/The magnet plates contain strong neodymium magnets that must be handled with extreme
care.
/When storing magnets in proximity to other magnets or materials that are attracted to mag-
nets, take precautions so that objects do not slam together. Neodymium magnets are britt-
le and can shatter or crack, sometimes producing dangerous fragments moving at high
speeds. Fingers can also be severely pinched between magnets or between magnets and
certain metals.
/Keep the magnetic drives spatially separated and DO NOT put the drives together at any
time. Due to the magnetic force, placing them in close proximity can cause them to “crash”
together, resulting in damage to the drive magnets and/or structure.
/Persons with pacemakers or similar medical devices should not come near Neodymium
magnets.
/Neodymium magnets can damage magnetic media such as credit cards, magnetic ID cards,
televisions, computer memory and computer monitors. Keep magnets at least 30 cm
(12 inch.) from these devices away.
/Neodymium magnets should not be burned or machined. They will lose their magnetic pro
perties if heated above 80 °C (175 °F). DO NOT AUTOCLAVE the magnetic drives.
/Neodymium magnets are not toys. The magnetic drives should only be used for their inten-
ded purpose. Children should not be allowed to play with them.
MULTI-MAGPEN INSTRUCTION MANUAL

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
1/ INTENDED USE
The Multi-MagPen is a general laboratory product for 3D cell culture to be used by qualied personnel
in a laboratory environment. It is intended for research use only and not approved for human or animal
use.
MULTI-MAGPEN KIT & SLEEVE
Item No. Product Description Content/Packaging
657896 96 Well Multi-MagPen Kit
96 Well Multi-MagPen Drive, transparent, non-
sterile, 1 pcs./box, 96 Well Multi-MagPen Sleeve,
PP, transparent, sterile, Cell Repellent,
1 pcs./bag, 2 pcs./box
651596 96 Well Multi-MagPen Sleeve 96 Well Multi-MagPen Sleeve, PP, transparent,
Cell Repellent, 1 pcs./bag, 10 pcs./box
2/ MATERIALS
Multi-MagPen Sleeve
Multi-MagPen Drive
Cell Repellent Plate
(not included in the Kit)

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
1
Instructions to transfer 3D cell cultures using the 96 Well Multi-MagPen
To assure sterility clean the Multi-MagPen Drive with 70 % ethanol. DO NOT autoclave the Multi- MagPen
Drive (see also safety precautions). During the cellular transfer process, the Multi-MegPen Drive is
inserted into the sterile Multi-MagPen Sleeve. Hence the Multi-MagPen Drive is never in direct contact
with the cell culture itself.
If media must be removed from the
wells, the microplate should be placed
on the 96 Well Holding Drive (part
of the 96 Well Bioprinting Kits; Item
No. 655840/-41) to secure the 3D cell
cultures at the bottom of the wells dur-
ing pipetting.
Prepare Receiver Plate
The receiver plate should contain the same liquid
volume as the donor plate (100 - 200 µl/well). If the
media volume is too low, the Multi-MagPen Sleeve
will not touch or dip in the liquid and 3D cell cultures
will not be transferred properly to the new plate. If
the media volume is too high, media will spill out the
well as soon as the Multi-MagPen Sleeve will dip in
and 3D cell cultures might get lost.
Prepare Donor Plate
To assure liquid contact and reliable attachment
of the 3D cell cultures to the Multi-MagPen Sleeve
the recommended working volume per well is
100 – 200 μl. If the media volume is too low, the
Multi-MagPen Sleeve will not touch or dip in the
liquid and 3D cell cultures will not attach to it. If the
media volume is too high, media will spill out the well
as soon as the Multi-MagPen Sleeve will dip in.
2
3/ INSTRUCTIONS

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
Thereafter insert the magnetic Multi-MagPen
Drive into the Multi-MagPen Sleeve.
4
Carefully make 2-3 circular movements with
the plate on the desk to facilitate 3D cell culture
attachment to the Multi-MagPen Sleeve.
5
Gently lift the Multi-MagPen Drive with its sleeve
off the plate. The magnetized 3D cell cultures will
stick to the Multi-MagPen Sleeve and will be held
by the magnetic force of the Multi-MagPen Drive
during transfer.
6
3
Take off the lid of the donor plate (microwell plate
containing 3D cell cultures or spheroids) and insert
the sterile Multi-MagPen Sleeve in the wells of the
plate.
Place the combined Multi-MagPen Drive and
Multi-MagPen Sleeve into the receiver plate or
where you want to transfer the cells to.
7

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
Take out the Multi-MagPen Drive from the
Multi-MagPen Sleeve.
WARNING
DO NOT place the receiver plate still containing the Multi-MagPen Drive in the
Multi-MagPen Sleeve on the Holding Drive. Due to the magnetic force they
can strongly attract each other, and separation of the components becomes
dicult and may damage the magnet drives.
Tap lightly with the at of your hand on the
Multi-MagPen Sleeve. This will mechanically detach
the 3D cell cultures from the Multi-MagPen Sleeve in
addition to the magnetic force of the Holding Drive.
Remove the Multi-MagPen Sleeve and cover the
receiver plate with its original lid.
Place the receiver plate containing only the
Multi-MagPen Sleeve on the 96 Well Holding Drive
(part of the 96 Well Bioprinting Kits; Item No.
655840/-41). The 3D cell cultures will detach from
the Multi-MagPen Sleeve by the magnetic force of
the Holding Drive and settle on the bottom of the
well.
8
9
10

Greiner Bio-One GmbH Frickenhausen, Germany
PHONE [phone] / FA X [phone] / E-MAIL in[email protected] IFU F076057 EN [rev.00, 07.2021]
Problem Probable Case Solution
A few 3D cell cultures remain in
the donor plate
Remaining 3D cell
cultures did not
adhere suciently
to the Multi-MagPen
Sleeve
Repeat step 3 to 9
If available, you can use the
Single-MagPen (Item No. 657850)
for the relocation of single 3D cell
cultures
A few 3D cell cultures remain
attached to the Multi-MagPen
Sleeve
Remaining 3D cell
cultures did adhere
strongly to the
Multi-MagPen Sleeve
Put the Multi-MagPen Sleeve again
into the receiver plate (which is still
located on the Holding Drive) and
repeat step 9 and 10 in order to
improve the detachment of the 3D
cell cultures
Inconsistent 3D cell culture
transfer from the donor plate
to the receiver plate
Magnetization of the
3D cell culture is too
low
Increase concentration of
NanoShuttle
96 Well Holding Drive is not
available
Customer has not
purchased a 96 Well
Bioprinting Kit
The Multi-MagPen Drive can serve
to detach 3D cell cultures from
the Multi-MagPen Sleeve. After
removing the Multi-MagPen Drive
from the Multi-MagPen Sleeve,
position it under the receiver plate
and to use its magnetic force to
release the 3D cell cultures from
the Multi-MagPen Sleeve
4/ TROUBLESHOOTING

www.gbo.com
Devices of Greiner Bio-One are to be used by properly qualied persons only in
accordance with the relevant Instructions for Use (IFU), where applicable.
For more information contact your local Greiner Bio-One sales representative or visit our
website (www.gbo.com).
All information is provided without guarantee despite careful processing. Any liability, warranty
or guarantee of Greiner Bio-One GmbH is excluded. All rights, errors and changes are reserved.
If not stated otherwise, Greiner Bio-One GmbH has all copyrights and/or other (user-)rights in
this documents, in particular to signs such as the mentioned (word-picture-)brands and logos.
Any use, duplication or any other use of the rights of Greiner Bio-One GmbH is expressly prohibited.
Media owner: Greiner Bio-One GmbH / Represented by Managing Directors Jakob Breuer and
Heinz Schmid. The company is registered in the Commercial Register at the r st instance court
in Stuttgart, HRB 224604 / VAT Number: DE812585719.
F076057 English [rev.00, 07.2021]
GREINER BIO-ONE GMBH
FRICKENHAUSEN, GERMANY
PHONE  +497022948-0
FAX +497022948-514
E-MAIL in[email protected]
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