Hach TenSette plus User manual

DOC022.98.00744
TenSette plus
08/2018, Edition 2
User Manual
Bedienungsanleitung
Manuel de l'utilisateur
Gebruikershandleiding

English..............................................................................................................................3
Deutsch.......................................................................................................................... 17
Français......................................................................................................................... 33
Nederlands....................................................................................................................49
2

Table of contents
Specifications on page 3 Operation on page 7
General information on page 3 Calibration on page 12
Installation on page 5 Maintenance on page 14
Configuration on page 6 Troubleshooting on page 16
Legal information
Manufacturer: Thermo Fisher
Distributor: Hach Lange GmbH
The translation of the manual is approved by the manufacturer.
Specifications
Specifications are subject to change without notice.
Specification Details
Enclosure Pipette: Mechanically and chemically resistant materials (indoor use only)
Tips: natural color polypropylene
Weight 210 g (with battery installed)
Battery Li-ion battery, 950 mAh
Charging temperature 0 to 45 °C (32 to 140 °F)
Power adapter Input voltage: 100–240 V, 50–60 Hz, 180 mA
Output voltage: 5.0 V–0.5 A
Operating temperature 15 to 35 °C (59 to 95 °F)
Storage temperature ≤ 1 month: -20 to 45 °C (-4 to 140 °F) (60±25% relative humidity)
> 1 month: -10 to 20 °C (14 to 68 °F) (60±25% relative humidity)
Relative humidity 20 to 85%, non-condensing
Altitude 2000 m (6562 ft) maximum
Certifications CE
Warranty 1 year (EU: 2 years)
General information
In no event will the manufacturer be liable for direct, indirect, special, incidental or consequential
damages resulting from any defect or omission in this manual. The manufacturer reserves the right to
make changes in this manual and the products it describes at any time, without notice or obligation.
Revised editions are found on the manufacturer’s website.
Safety information
N O T I C E
The manufacturer is not responsible for any damages due to misapplication or misuse of this product including,
without limitation, direct, incidental and consequential damages, and disclaims such damages to the full extent
permitted under applicable law. The user is solely responsible to identify critical application risks and install
appropriate mechanisms to protect processes during a possible equipment malfunction.
English 3

Please read this entire manual before unpacking, setting up or operating this equipment. Pay
attention to all danger and caution statements. Failure to do so could result in serious injury to the
operator or damage to the equipment.
Make sure that the protection provided by this equipment is not impaired. Do not use or install this
equipment in any manner other than that specified in this manual.
Use of hazard information
D A N G E R
Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.
WARNING
Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death or serious
injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or moderate injury.
N O T I C E
Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special
emphasis.
Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument
could occur if not observed. A symbol on the instrument is referenced in the manual with a
precautionary statement.
This symbol, if noted on the instrument, references the instruction manual for operation and/or safety
information.
Electrical equipment marked with this symbol may not be disposed of in European domestic or public
disposal systems. Return old or end-of-life equipment to the manufacturer for disposal at no charge to
the user.
Chemical and Biological Safety
D A N G E R
Chemical or biological hazards. If this instrument is used to monitor a treatment process and/or
chemical feed system for which there are regulatory limits and monitoring requirements related to
public health, public safety, food or beverage manufacture or processing, it is the responsibility of the
user of this instrument to know and abide by any applicable regulation and to have sufficient and
appropriate mechanisms in place for compliance with applicable regulations in the event of malfunction
of the instrument.
Normal operation of this device may require the use of chemicals or samples that are biologically
unsafe.
• Observe all cautionary information printed on the original solution containers and safety data
sheets prior to their use.
• Dispose of all consumed solutions in accordance with the local and national regulations and laws.
• Select the type of protective equipment suitable to the concentration and quantity of the dangerous
material being used.
Product overview
The Hach TenSette plus is an electronically assisted pipette for a wide range of liquid handling
operations. Use the Hach TenSette plus with original Hach pipette tips (Item no. BBP068). Thanks to
4 English

the electronic motor and electronic control, pipetting is easy and comfortable, yet still fast and
accurate. It operates on the air displacement principle (i.e., an air interface) and uses detachable,
disposable tips, which are easy to remove with a soft touch tip ejector.
The adjustable, index finger-operated pipetting trigger uses natural hand movement. The Hach
TenSette plus provides many functions that are very practical for daily use in laboratory work, such
as forward, reverse and repetitive pipetting, stepper and diluting modes.
The adjusted delivery volume is clearly indicated in the LCD display on top of the handle. The long
lasting lithium ion battery is always charged with rapid charge technique.
All pipettes are factory calibrated. A Pipette Inspection Report, which covers calibration conditions
and results, is delivered with the pipette.
Product components
Make sure that all components have been received. If any items are missing or damaged, contact the
manufacturer or a sales representative immediately.
• Hach TenSette plus (supplied with installed rechargeable battery, Item no. BBP089)
• Charger (includes external power supply 100–240 V, 50/60 Hz, 15 mmA and power plug adapters
for EU, UK, US, Australia)
• Shelf hanger
• Spare O-ring and tube of grease
• Sample Hach pipette tips
Installation
CAUTION
Multiple hazards. Only qualified personnel must conduct the tasks described in this section of the
document.
Connect to power
WARNING
Explosion and fire hazard. Battery substitution is not permitted. Use only batteries that are supplied by
the instrument manufacturer.
WARNING
Fire and explosion hazard. Lithium batteries may get hot, explode or ignite and cause serious injury if
exposed to abuse conditions.
N O T I C E
Use only the original Hach TenSette plus charger and battery.
The pipette battery must be charged before initial use. You can use the pipette while the charger is
connected. The charging time is typically less than one hour. An indicator in the LCD display shows
the charge level of the battery. When the indicator shows an empty battery, pipetting is not possible,
and the pipette must be charged again.
1. Connect the lead of charger to the socket on the back of the pipette.
2. Connect the charger to an AC wall socket.
Note: If the battery is completely empty, it may take a few minutes before the pipette will turn on.
English 5

Adjust the trigger position
The index finger-operated trigger, which activates the piston movement, can be adjusted by rotating
it 60 degrees in both directions from the center position. Usually right-handed operators turn it left
(counter-clockwise) to get the best possible position for the thumb to eject the tip. Refer to Figure 1.
Figure 1 Adjust the trigger position
1 Trigger 2 Trigger and finger rest adjustable
Tip ejection
To help eliminate the risk of contamination, the pipette is fitted with a tip ejector system. The tip
ejector system consists of a soft-touch tip ejector with a specially designed gearing mechanism.
Refer to Figure 2 on page 7.
To release the tip, point the pipette at a suitable waste receptacle and press the tip ejector with your
thumb.
Shelf hanger
You can attach the pipette shelf hanger on a counter, pipette stand or anywhere where you want to
hang your pipette.
1. Clean the area where you plan to attach the shelf hanger.
2. Apply the two stickers to the underside of the shelf hanger.
3. Press the shelf hanger firmly into place—on a shelf, countertop or pipette stand.
4. Hang the grippy finger rest on the shelf hanger.
Configuration
Select the pipette functions and speed
1. Use the left selection key to select Menu to choose the pipetting function. Refer to Figure 2.
2. Use the right selection key to scroll the function list and select the function with OK.
3. Select one option.
Option Description
Pippete Forward technique. Refer to Pipette on page 8.
RPipet Reverse and repetitive technique. Refer to RPipet on page 8.
Stepper Multi-dispensing technique. Refer to Stepper on page 9.
Dilute Dilution technique. Refer to Dilute on page 9.
6 English

Option Description
Program Select the program number from 1 to 9. Default volumes: 0.2, 0.4, 0.5, 1.0, 1.3, 2.0, 2.3, 2.5,
5.0 mL
Options Calibrate: Calibration mode. Select between:
• Two points: two points calibration.
• One point: one point calibration.
• Counter: Displays number of pipettings since last calibration.
Service: The piston can be disconnected and reconnected in Service mode. Refer to Maintenance
on page 14.
Name: This function enables user to set a name for the pipette. The name is always shown on the
display when the pipette is in sleep mode. To change the default name:
1. Choose Name from the menu and select Edit. The first digit starts to flash.
2. Change the digit with the scroll key. Move to the next digit with OK. When the last digit is
accepted the name is changed.
Power: With this function the power can be turned off. Pressing any key turns on the power.
Buzzer: The buzzer can be turned on and off with this function.
Version: The software version is displayed.
Backlight: Turn on/off the backlight.
Figure 2 Pipette operation
1 Speed indicator 4 Function 7 Scroll Key
2 Battery indicator 5 Speed-in/-out 8 Tip Ejector Lever
3 Volume indicator 6 Right Selection Key 9 Left Selection Key
Operation
WARNING
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
English 7

Pipette
1. Choose the Pipette function.
2. Select the pipetting volume simply by pressing the scroll key up or down.
3. Accept the volume with OK. Optionally, select Speed (1 slow to 9 fast) and the speed-in starts to
blink.
4. Select the speed-in with the scroll key and accept with OK.
5. Select the speed-out with the scroll key and accept with OK.
6. Fill a clean reagent reservoir with the liquid to be dispensed.
7. Dip the tip under the surface of the liquid in the reservoir and press the trigger. The liquid is drawn
into the tip.
8. Wait until the liquid is not moving in the tip and withdraw the tip from the liquid touching it against
the edge of the reservoir to remove excess liquid.
9. To deliver the liquid, press the trigger. The blow-out is also included to empty the tip.
10. Change the tip and continue pipetting.
RPipet
With the RPipet function both, reverse and repetitive techniques, are possible.
1. Choose the RPipet function.
2. Press the scroll key up or down to select the pipetting volume.
3. Accept the volume with OK.
4. Select Speed and the speed-in starts to blink.
a. Select the speed-in with the scroll key and accept with OK.
b. Select the speed-out with the scroll key and accept with OK.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency to
foam easily. The technique is also recommended for dispensing very small volumes.
1. Fill a clean reagent reservoir with the liquid to be dispensed.
2. Dip the tip under the surface of the liquid and press the trigger. This action will fill the tip.
3. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid.
4. Deliver the preset volume by shortly pressing the trigger. Some liquid will remain in the tip and
this should not be included in the delivery. Blowout is displayed.
5. To empty the tip, press the trigger again.
6. Change the tip and continue pipetting.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume.
1. Fill a clean reagent reservoir with the liquid to be dispensed.
2. Dip the tip under the surface of the liquid and press the trigger. This action will fill the tip.
3. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid.
4. Deliver the preset volume by pressing and holding the trigger down. Some liquid will remain in the
tip and this should not be included in the delivery.
5. Dip the tip again to reagent reservoir and release the trigger. This action will fill the tip again.
8 English

6. Continue pipetting by repeating steps 3 and 4.
7. To empty the tip completely, dispense by shortly pressing the trigger. Some liquid will remain in
the tip and this should not be included in the delivery. Blowout is displayed.
8. To empty the tip completely, press the trigger again.
9. Change the tip and continue pipetting.
Stepper
With the Stepper function repeated dispensing of selected volume is possible.
1. Choose the Stepper function.
2. Push the scroll key up or down to select the pipetting volume.
Note: The display shows always the maximum number of steps during volume selection.
3. Accept the volume with OK.
4. Choose the number of steps with the scroll key and accept with OK.
Note: Optionally select the speed.
5. Select Speed and the speed-in starts to blink.
6. Select the speed-in with the scroll key and accept with OK.
7. Select the speed-out with the scroll key and accept with OK.
8. Fill a clean reagent reservoir with the liquid to be dispensed.
9. Dip the tip under the surface of the liquid and press the trigger. This action will fill the tip.
10. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid.
11. Deliver the preset volume by pressing the trigger. The display shows the number of steps left.
12. Continue dispensing by repeating step 9. After the last step, Blowout is displayed.
13. To empty the tip completely, press the trigger.
14. Change the tip and continue pipetting.
Note: The pipette can be emptied at any time by pressing Cancel (left selection key, refer to Figure 2
on page 7).
Dilute
With the Dilute function dispensing of two selected volume is possible.
1. Choose the Dilute function. The first volume (VOL 1) is shown on display.
2. Push the scroll key up or down to select the first volume.
3. Accept the volume with OK.
4. Select the second volume (VOL 2) with the scroll key and accept with OK.
Note: Optionally select the speed.
5. Select Speed and the speed-in starts to blink.
6. Select the speed-in with the scroll key and accept with OK.
7. Select the speed-out with the scroll key and accept with OK.
8. Fill a clean reagent reservoir with the liquid to be dispensed.
9. Dip the tip under the surface of the first liquid in the reservoir and press the trigger. The first
volume is drawn in to the tip. Air (air buffer) is displayed.
10. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid. Press the trigger again to aspirate air buffer.
11. Dip the tip under the surface of the second liquid and press the trigger. The second volume is
drawn in to the tip.
12. Withdraw the tip from the liquid.
English 9

13. To deliver both volumes, press the trigger. The blow-out is also included to empty the tip.
14. Change the tip and continue pipetting.
Program
Programs are stored settings that can be edited, stored and retrieved. Under the Program function
the following volumes are default: 0.2, 0.4, 0.5, 1.0, 1.3, 2.0, 2.3, 2.5 and 5.0 mL. When working with
the mode Program, the scroll key selects the program and it is a very fast way to switch between
different settings. Additional functions such as mixing, counter, etc. are also available in the mode
Program. To change the default volumes, choose Edit.
It is also possible to replace default volumes by additional functions. Refer to Additional functions in
mode Program on page 10.
Additional functions in mode Program
Mix + Pipette
This function adds an automatic mixing step after normal pipetting.
1. Select Program > Edit, then select with the scroll key Mix + Pipette and accept with OK.
2. Select the desired volume with the scroll key and accept with OK.
3. Select the pipetting speeds accordingly. After dispensing the volume, Mix is displayed. By
pressing the trigger the pipette starts to pipette approximately 70% of the selected volume several
times as long as the trigger is held pressed. After releasing the trigger the pipette stops after next
dispensing and Blowout is displayed.
A normal blow-out function is done by pressing the trigger and the pipette is again ready for next
pipetting.
Pipette + Count
This function adds an automatic count number to each pipetting step.
1. Select Program > Edit, then select with the scroll key Pipette + Count and accept with OK.
2. Select the desired volume with the scroll key and accept with OK.
3. Select the pipetting speeds accordingly.
4. Next choose the maximum number of pipettings, the default value is 999.
Note: After the maximum number of pipettings is reached, the counter returns to zero. The counter can be
reset at any time to zero by pressing the scroll key down > OK; scroll key up > OK.
Seq stepper
The sequential stepper mode enables serial dispensing of different volumes (in normal stepper mode
only fixed volume).
1. Select Program > Edit, then select with the scroll key Seq stepper and accept with OK.
2. Select the amount of dispensings (maximum 20) with the scroll key and accept with OK. VOL 1 is
displayed and the highest possible volume is flashing.
3. Select the first volume with the scroll key and accept with OK. Now VOL 2 is displayed and the
highest possible volume is flashing.
4. Select the second volume with the scroll key and accept with OK. After selecting the last volume,
the total volume is shown on the display and speed-in is flashing. Select the pipetting speeds and
the pipette is ready for pipetting.
5. Fill a clean reagent reservoir with the liquid to be dispensed.
6. Dip the tip under the surface of the liquid in the reservoir and press the trigger. This action will fill
the tip and the first volume is displayed.
7. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid.
10 English