Hanna Instruments HI 5315 User manual

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HI 5315HI 5315
HI 5315HI 5315
HI 5315
Reference Electrode
Instruction Manual
HI 5315
REFERENCE

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HI5315ReferenceElectrodeHI5315ReferenceElectrode
HI5315ReferenceElectrodeHI5315ReferenceElectrode
HI5315ReferenceElectrode
I.I.
I.I.
I. IntroductionIntroduction
IntroductionIntroduction
Introduction
The Hanna HI 5315 reference electrode is a silver-silver
chloridedoublejunctionhalf-celldesignedforuse withion
selectiveelectrodesorpHsensors. Theelectrodeutilizesa
quickflushsleevedesign.Theexternalreferenceelectrolyte
chamber is refillable. The internal chamber is a perma-
nentlyfilled,gelstabilized,chloridecontainingelectrolyte.
IIII
IIII
II. SpecificationsSpecifications
SpecificationsSpecifications
Specifications
Type: Ag/AgCl
Operating Temperature: 0-85°C
Operating pH: 0to14pH
Dimensions: 12mm(OD)x120mm
insertion(0.47”x4.72”)
Connection: Banana Plug
WettedMaterials: PEI
E°SHE (calculated): 0.238V
III.III.
III.III.
III. Theory of operationTheory of operation
Theory of operationTheory of operation
Theory of operation
Areferencehalf-cellprovidestheelectrolyticcontactneces-
sarytopermitavoltagegradienttobemeasuredacrossa
measurementmembrane,suchasanISE. AAg/AgClelec-
trode is the most common type of reference. The Nernst
expressionforthistypehalf-cellisexpressedintheequa-
tion below. Note that the potential is a function of the
chlorideconcentration.
E=EoAgCl -0.059log[Cl--
--
-]
Achlorideelectrolytecompletestheelectricalcircuitinthe
innerhalf-cell. Abridgeelectrolyteisusedasabufferzone
betweentheinnerhalf-cellandthesample.Asmallstable
liquidjunctionvoltageisgeneratedwherethesampleand
electrolyte come into contact.The size of this voltage,the
identityofthediffusingionsandhowthisbarrierisformed
dictate stability. The barrier zone for this reference is a
narrow ring shaped opening formed between the outer
sleeveandtheloweredgeoftheskirtshapedinnerbody.

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IVIV
IVIV
IV.....HI 5315 design elementsHI 5315 design elements
HI 5315 design elementsHI 5315 design elements
HI 5315 design elements
V.V.
V.V.
V. Reference Fill SolutionsReference Fill Solutions
Reference Fill SolutionsReference Fill Solutions
Reference Fill Solutions
The fillsolutionusedintheexternalelectrolytechamberof
this electrode is replaceable. Table 1 lists Hanna‘s ion
selectiveelectrode(ISE)offering alongwiththerecommeded
fill solutions used when making an ion measurement. A
bottleofthissolutionisprovidedwiththeISEwhenshipped.
The fillsolutions listed will notinterferewith the sensing
electrodeorreactwiththesample.Theyareformulatedto
minimize liquid junction potentials and to produce the
moststableand reproducibleelectrodepotential. ThThwb
Verify you are using the correct fill solution with your
electrode.ab
Upper Cap
Spring
O-Ring
Ceramic Junction
on Inner Stem
Fill Hole
O-Ring and
Plug
Liquid junction

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d reproducible potential.
VI.VI.
VI.VI.
VI. Electrode Preparation:Electrode Preparation:
Electrode Preparation:Electrode Preparation:
Electrode Preparation:
1. RemovetheParafilm® seal wrappedovertheceramic
junctiononinnerstemanddiscard.Thisisonlyused
forshipping and long termstorage.
2. Rinseinnerbodywithdeionizedwatermakingcertain
toweto-ringfoundontheinnerstem.
1ELBAT1ELBAT 1ELBAT 1ELBAT1ELBAT
edoCnoitpircseDnoituloSlliF
2004IHllec-flahedimorBIH2707
3004IHllec-flahmuimdaCIH2707
4004IHllec-flahmuiclaCIH2807
7004IHllec-flahedirolhCIH2707
8004IHllec-flahcirpuCIH2707
9004IHllec-flahedinayCIH2707
0104IHllec-flahediroulFIH5707
1104IHllec-flahedidoIIH2707
2104IHllec-flahetafluS/daeLIH2707
3104IHllec-flahetartiNIH8707
4104IHllec-flahmuissatoPIH6707
5104IHllec-flahedifluS/revliSIH2707
Recommended Fill Solutions for ISERecommended Fill Solutions for ISE
Recommended Fill Solutions for ISERecommended Fill Solutions for ISE
Recommended Fill Solutions for ISE
Deionized
Water
Remove
Parafilm

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3. Reassemble electrode by gently pushing the inner
assembly into the outer body (from the bottom).
Slide the spring and upper cap down the cable and
over the top of the inner stem. Screw the upper cap
into place.
4. Unscrewandremovethefillholeplugando-ringon
fillholespout.
5. Selecttheappropriatefillsolutionfrom Table1. Use
thedropperpipetteprovidedtoaddafewdropsoffill
solutiontotheelectrode.Invertelectrodetowettheo-
ringandrinse theelectrolytechamber.
6. Holding the body of the electrode gently press the
upper cap with your thumb. This permits the fill
solutiontodrainoutofthebody.Releaseyourthumb
and verify electrode returns to its original position.
(Youmayneedtogentlyassistforthistooccur).
7. Tightentheupperelectrodecapontothebodyandfill
electrode body until the fill solution volume is just
belowthefillhole.
8. Position the reference electrodewith appropriate ion
selectiveelectrode(ISE)inaHannaHI76404electrode
holder (or equivalent) and connect banana plug to
thereferencejackoftheISEmeter.
HI5315
REFERENCE

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VII.VII.
VII.VII.
VII. General GuidelinesGeneral Guidelines
General GuidelinesGeneral Guidelines
General Guidelines
•During measurement always operate electrode with
thefillholeopen.
•Verifyprotectivecaphasbeenremoved.
•Duringnormaluse,fillsolution will slowlydrainout
of the tapered cone junction at the bottom of the
electrode.Excessiveloss(>4cmdropwithin24hours)
is not normal. If this occurs verify the upper cap is
tightenedandtheinterfacebetweentheinternalcone
andouterbodyisfreeofdebris.
•Add fill solution daily to maintain a good head
pressure.Foroptimum reference response, this level
should be maintained and not be allowed to drop
morethan2-3cm(1-inch)belowfillhole.
•Whenchangingfromonefillsolutiontoanother,drain
1st fill solution, rinse with an aliquot of the new
solution,drain,thenrefillwithnewfillsolution.
•Components found in the fill solutions should not
interferewiththeionbeingmeasured.Verifythecorrect
solution is being used.
•Donotuseanelectrodeifcrystallizedsaltsarevisible
insidetheelectrode.Drainelectrode,disassembleand
rinseinternalbodywithdeionizedwater.Reassemble
andrefillwithfreshfillsolution.
•Ifanerraticmeasurementoccurs,checktoseeifforeign
matterisseentrappedneartheinternalcone. Drain
andrefillwithfreshfillsolution.
•Theinternalchamberofthiselectrodeisgelfilled.If
theelectrodehasbeenleftdryforlongperiodsoftime
thegelmaybedehydratedgivingunstablereadings.
Disassembleelectrodeandsoakinternalassemblyin
HI 7075 fill solution. Verify the ceramic junction is
wetted by the fill solution. Warming the solution
slightly (50°C) before soaking it will hasten this
process.Permittheelectrodetocoolcompletelywhile
immersedinthis solution.

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VIII.VIII.
VIII.VIII.
VIII.Disassembly - AssemblyDisassembly - Assembly
Disassembly - AssemblyDisassembly - Assembly
Disassembly - Assembly
Disassembly is normally not required or recommended. If
crystals or other matter are seen between outer body and
innercone,theelectrodemaybedisassembledforcleaning
or inspection. Protect the cone surface from oils or dirt by
usingglovesorlabtissues.
1. Drain fillsolutionfromelectrodebodybydepressing
the uppercapwhileholdingthe outer body.
2. Rinse inner body with deionized water and drain by
pressing the upper cap.
3. Unscrew upper cap and slide cap and spring up the
sensorcabletowardtheconnector.
4. Pushtheinnerbodythroughtheoutersleevebygently
pushingdown on the innerbodynear the threads.
5. Grasptheconeandgentlypullassemblyoutofsleeve.
6. Conemaybecleanedwithdeionizedwaterandasoft
labtissue(HI731318).
7. Cleanandinspectinternalsurfaceof outerbody.
8. Reassemble electrode by gently pushing the inner
stem assembly into the outer body, sliding spring
down cable, and screwing cap into place. (Refer to
SectionVI;ElectrodePreparation).
9. Refill electrolyte, flush junction, and then top off
electrolyte.
IXIX
IXIX
IX. StorageStorage
StorageStorage
Storage
The HI 5315 reference electrode may be stored in air or
aqueoussolutionsbetweensamplemeasurements.
If the electrode will be used frequently and needs to be
readyforuse,takemeasurestopreventevaporationoffill
solution. Top off fill solution, replace o-ring and fill hole
plugonthefillholeopening,andplaceprotectivecapover
junction tip. Store electrode upright. Before using, flush
junctiononceandtopofffillsolution.
Forlongtermstorage,disassembleelectrodeandwashall
salts from assembly with deionized water. Wrap ceramic
junction on inner stem with Parafilm®or other sealing
film. Store dry and disassembled.

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MAN5315 07/06REV4
WARRANTYWARRANTY
WARRANTYWARRANTY
WARRANTY
Hanna Instruments ISE series electrodes are warranted to be free of
defectsinmaterialandworkmanshipfor6monthsfromdateofpurchase
when used for their intended purpose and maintained according to
instructions. If they fail to work when first used contact your dealer
immediately. Damage due to accidents, misuse, misapplication,
tamperingorlackofprescribedmaintenanceisnotcovered.
HannaInstrumentsreservestherighttomodifythedesign,construction
or appearance of its products without advance notice.