Hanna Instruments HI 84429 User manual

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Instruction Manual
HI 84429
TITRATABLE ACIDITY
MINITITRATOR & pH METER
for Dairy Products
www.hannainst.com

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All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at [email protected].
This instrument is in compliance with directives.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION................................................................................................. 3
GENERAL DESCRIPTION ........................................................................................................ 3
SPECIFICATIONS ................................................................................................................... 6
PRINCIPLE OF OPERATION .................................................................................................... 8
FUNCTIONAL DESCRIPTION................................................................................................... 9
TITRATOR STARTUP ........................................................................................................... 11
SETUP CONFIGURATION MENU .......................................................................................... 12
ELECTRODE PREPARATION .................................................................................................. 18
ELECTRODE CALIBRATION PROCEDURE................................................................................. 19
pH BUFFER TEMPERATURE DEPENDENCE............................................................................ 23
PUMP TUBE REPLACEMENT ................................................................................................ 24
PURGE .............................................................................................................................. 24
PUMP CALIBRATION PROCEDURE ........................................................................................ 26
TITRATION PROCEDURE ..................................................................................................... 29
pH MEASUREMENT ............................................................................................................ 34
TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only) ............................... 38
PC INTERFACE ................................................................................................................... 40
FUSE REPLACEMENT .......................................................................................................... 43
TROUBLESHOOTING GUIDE ................................................................................................ 44
ELECTRODE CONDITIONING & MAINTENANCE ...................................................................... 45
ACCESSORIES .....................................................................................................................46
WARRANTY........................................................................................................................47

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Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occured during shipment, please notify your Dealer.
Each HI 84429 minititrator is supplied complete with:
•FC 260B pH electrode
•HI 5315 Reference electrode
•HI 7072 Filling solution (30 mL)
•HI 7662-T Temperature probe
•HI 84429-50 Titrant (100 mL)
•HI 84429-55 Standard (500 mL)
•HI 700640 Cleaning solution for milk deposits (2x20mL)
•pH 4.01 buffer solution (230 mL)
•pH 6.00 buffer solution (230 mL)
•pH 8.30 buffer solution (230 mL)
• Two 50 mL beakers
• Two 20 mL beakers
• Tube set with cap
• Stir bars (2 small & 2 large)
• Power cord
• One 1 mL syringe
• One capillary dropper pipette
• Instruction manual
Note:Save all packing material until you are sure that the instrument works correctly.
Any defective item must be returned in its original packing.
PRELIMINARY EXAMINATION
The HI 84429 is a valuable, easy to use, microprocessor-based automatic titrator and pH meter that
benefits from Hanna’s years of experience as manufacturer of analytical instruments.
It has a simple and accurate peristaltic pump to ensure the best accuracy and repeatability. By performing
pump calibration with the provided Hanna standards, the instrument accuracy is assured.
The instrument comes with a preprogrammed analysis method designed for Total Titratable Acidity
measurements on milk.
The HI 84429 performs automatic analysis, all the necessary calculations and assures to the user a simple
and effective interface.
The instrument has a powerful and effective built-in algorithm to analyze the shape of the pH electrode
response and to determine the reaction completion.
By simply pressing the Start key, the instrument will automatically titrate the acidity up to the end point.
The result is immediately displayed in convenient units, then the instrument is ready for another titration.
GENERAL DESCRIPTION

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Other features:
• Log on demand up to 100 samples (50 for pH measurement; 50 for titration results)
• GLP feature, to view last calibration data for pH electrode and pump
• PC interface
MEASUREMENT SIGNIFICANCE
Both pH and titratable acidity are used to measure milk acidity. A decrease in pH or increase in
titratable acidity indicates lactic fermentation has occurred, most likely due to bacterial activity. pH
and titratable acidity measurements, together with other tests available to the analyst, provide a
mechanism to ensure quality and freshness of the milk products.
The pH of milk (or other dairy products) is a measurement of the actual acidity of the milk at the time
of measurement. The measurement uses a pH electrode and pH meter that reads out directly in units
of pH after calibrating the electrode and meter together using pH buffers. The pH of fresh milk is
slightly acidic typically falling between 6.5 to 6.7 pH at 25°C.
Titratable acidity measures the total titratable acidity and differs from pH as it also includes the
buffering capacity of the milk constituents. Titratable acidity in dairy products, is determined by
titrating a sample with sodium hydroxide to a fixed endpoint pH (pH 7.0 or a phenolphtalein
endpoint of pH 8.3). The actual neutralization of milk occurs at an endpoint of pH 7.00, however
standard methods utilize the phenolphtalein endpoint value. The results will differ depending upon
which endpoint is utilized. The endpoint can be determined visually using color change produced by
phenolphtalein indicator or less subjectively, using a pH electrode as the indicator in a potentiometric
acid-base titration.
Titratable acidity can be expressed in various units values basically expressed as a result of the
strength of the sodium hydroxide (NaOH) needed for titration:
Soxhlet Henkel degrees (°SH)- mostly used in Central Europe
This value is obtained by titrating 100 mL of milk with 0.25N NaOH, using phenolphtalein as indicator.
Thorner degrees (°Th)- mostly used in Sweden and the CIS
This value is obtained by titrating 100 mL of milk, thinned with 2 parks of distilled water, with 0.1N
NaOH, using phenolphtalein as indicator.
Dornic degrees (°D))
))
)- mostly used in Netherlands and France
This value is obtained by titrating 100 mL of milk, thinned with 2 parks of distilled water, with N/9
NaOH, using phenolphtalein as indicator.
Percent lactic acid (%l.a.))
))
)- frequently used in the UK, USA, Canada, Australia and New Zeeland
This value is obtained in the same way as °D, diving the result by 100.

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Taking into account the concentration of sodium hydroxide the result expressed into one unit value
can be easily converted into any other unit value according with the chart:
°SH °Th °D %l.a.
NaOH conc. (N) 0.25 0.1 0.111 0.0111
1 2.5 2.25 0.0225
0.4 1 0.9 0.009
4/9 10/9 1 0.01
The HI 84429 minititrator permits the measurement of both pH and titratable acidity of dairy products.
The titration method is a potentiometric end point determination at fixed pH value of 8.30.
The HI 84429 minititrator eliminates the subjective end point detection given by the human
eye, thus eliminating the errors that can appear in the results.
Acidity of dairy products can be measured in any of the unit values described previously by selecting
the desired unit value. Furthermore, by performing a pump calibration with the supplied standard
titrations with widely varying values may be made without changing the tubes or purging the titrant
in order to clean the tubes.

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SPECIFICATIONS
Titrator Range Titratable acidity (low range)
°SH: 0.0 to 15.0 °SH
°Th: 0 to 40 °Th
°D: 0 to 35 °D
%I.a.: 0.00 to 0.35 %I.a.
Titratable acidity HR (high range)
°SH: 10.0 to 75.0 °SH
°Th: 20 to 200 °Th
°D: 20 to 175 °D
%I.a.: 0.0 to 2.0 %I.a.
Resolution Titratable acidity (low range):
0.1 °SH
1 °Th
1 °D
0.01 %I.a.
Titratable acidity (high range):
0.5 °SH
1 °Th
1 °D
0.1 %I.a.
Accuracy 5% of reading
Titration method Acid-base titration
Principle End point titration, 8.30 pH
Pump debit 0.5 ml/min
Stirring speed 1500 rpm
Log data Up to 50 samples
pH meter pH meter -2.0 to 16.0 pH / -2.00 to 16.00 pH
pH Resolution: 0.1 pH / 0.01 pH
pH Accuracy: ± 0.01 pH
pH Calibration: 1, 2 or 3 point calibration;
3 available buffers (4.01, 6.00, 8.30)
Temperature manual or automatic from
compensation: -20 to 120 °C (-4 to 248 °F)
Log data Up to 50 samples
Temperature Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)

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Resolution 0.1 °C
Accuracy ±0.4 °C without probe error
REQUIRED REAGENTS
Code Description Quantity/Test
HI 84429-50 Titrant 0.5 mL
HI 84429-55 Standard 50 mL
Electrode FC 260B pH electrode (included)
HI 5315 reference electrode (included)
Temperature Probe HI 7662-T (included)
Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing
Power supply 220 V/50Hz; 115 V/60Hz; 10 VA
Dimensions 208 × 214 × 163 mm (8.2×8.4×6.4”) (with beaker)
Weight 2200 g (77 oz.)

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PRINCIPLE OF OPERATION
The determination of titratable acidity in dairy products is made according to a neutralization
reaction, that is the reaction between the acids found in dairy products and a base. This type of
reaction forms the basis of titration methods of analyzing acids.
Titratable acidity is measured on a sample by titrating the sample with sodium hydroxide to an endpoint
of 8.30 pH. The results are expressed in °SH, °Th, °D or %l.a. according with the setup selection.
For precise results it is very important to know the exact sample volume, titrant volume and
concentration. Also it is better to select the titration low range or high range according to the
expected acidity of the sample.
The peristaltic pump has good repeatability but the dosing volume depends on many factors including the
diameter of the tube or the tube stretching. To compensate, the pump needs to be calibrated. The calibration
of the pump is also needed in order to have high precision of the titrations.
The calibration procedure is the analysis of a known solution which has a known volume. By doing this,
the instrument makes a differential analysis between the standard and the dairy product sample. The pump
volumetric debit and the real concentration of the titrant is compensated.

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FUNCTIONAL AND PHYSICAL DESCRIPTION
FRONT PANELFRONT PANEL
FRONT PANELFRONT PANEL
FRONT PANEL
1. Titrant bottle 3. Keypad 5. Peristaltic pump tubes
2. Graphic LCD 4. Electrode holder
REAR PANELREAR PANEL
REAR PANELREAR PANEL
REAR PANEL
6. pH Electrode 11. Reference electrode socket 16. Power switch
7. Temperature probe 12. BNC electrode connector 17. Power cable connector
8. Reference electrode 13. Temperature probe socket 18. Peristaltic pump
9. Beaker 14. USB connector
10. RS232 connector 15. Fuse

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KEYPAD FUNCTION AND INDICATORS
ESC - used to leave the current screen and to return either to the previous screen or to the
main screen, depending on the context, while pressed in SETUP the new value of
the set parameter is not changed.
/ - used to modify a parameter’s value, to scroll the information displayed while
viewing a help or to move between the options from the instrument’s setup
HELP - used to access/leave the instrument’s contextual help
MENU - used to enter Setup, Recall or GLP selection menu, while instrument is in pH or
titration main screen
STIR - used to start/stop the stirrer.
Note: The stirer starts automatically during pump calibration and titration and cannot be
stopped by pressing STIR key.
1) Current time and instrument mode information (pH meter or Titrator)
2) pH electrode condition information
3) main reading information
4) instrument status information
GUIDE TO INCDICATORS
During the instrument’s operation a set of information are displayed on the LCD.
Displayed icons:
Unstable reading.
Stirrer on.
Pump activity.
Parameter can be changed.
1
5
2
3
4
9
6
8
7

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PERISTALTIC PUMP
Peristaltic pumps are self priming. Liquid never contacts the pump components. The titrant tubing
is pressed along the rotating rollers of the pump. The rollers compress the tubing drawing titrant
along and out the titrator tip.
TITRATOR STARTUP
• Place the instrument on a flat table. Do not place the instrument in direct sun light.
• Connect the instrument to mains socket with ground connection and the correct voltage and
frequency. See the label on the instrument rear for this.
• Turn the instrument ON using the power switch from the rear panel of the instrument.
• Set up the instrument. See the “Setup Configuration Menu” section for details.
• Prepare the electrodes and attach pH sensor and reference electrode to the instrument.
• Calibrate the pH electrode. At least a single point calibration in 8.30 pH buffer is necessary
for titration.
• Place the peristaltic pump tube on the pump. See the “Pump Tube Replacement” section for
the procedure.
• Remove the reagent bottle cap and place the bottle cap of the tubes. Place the reagent bottle
in the appropriate place on the titrator top.
• Connect the tubes with the peristaltic pump (inlet tube is connected with the reagent bottle,
outlet tube is connected with the dosing tip).
• Purge line.
• Calibrate the pump.
• Select Acidity titration range.
• Prepare the sample.
• Run a titration and log sample results.
5) functional key area
6) indicates that the displayed value can be changed using ARROW keys
7) temperature reading display
8) pH temperature compensation mode
9) stirrer and reading status area

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Option: Low Range, High Range.
From your knowledge of expected concentrations, use the
table below to determine which settings are appropriate.
Press the corresponding function key to select the
new option.
Note: The milk sample size will change with these settings:
Select Low Range for dairy products in the 0 to 15 ° SH range. Select High Range for dairy
product in the 10 to 75 ° SH range. The titrant solution for the High Range will remain the same.
Acidity titration
SETUP CONFIGURATION MENU
Option: ° SH, ° Th, ° D, % l.a.
Press the corresponding function key to change the
option.
Concentration unit
The titrator’s setup configuration menu may be accessed from the pH or titration screens by
pressing the Menu key, then Setup.
A list of setup parameters will be displayed with currently configured setting.
While in the setup menu it is possible to modify the instrument’s operation parameters. The
ARROW keys permit the user to scroll the setup parameters.
Press HELP to view the contextual help.
Press ESC to return to the main screen.
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Use the ARROW keys in order to increase/decrease
the value.
Press Accept to confirm or ESC to return to the
setup menu without saving the new value.
pH resolution
Option: 0.1 or 0.01.
Press the displayed function key in order to change
the option.
Option:
° C or ° F.
Press the function key in order to change the option.
Temperature unit
Option: 0 to 8.
Press Modify to access the backlight value.
Backlight
Calibration timeout
Option: Disabled or 1 to 7 days.
This option is used to set the number of days before
the pH calibration expired warning message is flagged.
Press Modify to access the calibration timeout value
modify parameter.

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Press / to highlight the value to be modified
(year, month, day, hour, minute or second). Use the
ARROW keys to change the value.
Press Accept to confirm the new value or ESC to
return to the setup.
This option is used to set the instrument’s date and
time.
Press Modify to change the date/time.
Date / Time
Contrast
Option: 0to 20.
This option is used to set the display’s contrast.
Press Modify to change the display’s contrast.
Use the ARROW keys or / in order to
increase/decrease the value.
Press Accept to confirm the value or ESC to return
to the setup menu.
Use the ARROW keys or / in order to
increase/decrease the displayed constrast.
Press Accept to confirm or ESC to return to the
setup menu.

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Press the corresponding function key to change the
option.
If the new selected language cannot be loaded, the
previously selected language will be reloaded.
If no language can be loaded the instrument will work in the “safe mode”. In “safe mode” all
the messages are displayed in English and tutorial and help information are not available.
Language
Use the ARROW keys to select the desired format.
Press Accept to confirm the value or ESC to return
to the setup menu.
Date format
Press Modify to change the Date Format.
This option is used to enable/disable tutorial mode.
If enabled this option will provide the user short
guides on the screen.
Press the function key to select this option.
Tutorial
Option: AM/PM or 24 hours.
Press the functional key to select the new value.
Time format

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Press Modify to access the baud rate screen. Use
ARROW to scroll through baud rate values and
press Accept to confirm the selection or ESC to
return to the setup menu without saving the new
value.
Options: 600, 1200, 2400, 4800, 9600 bps.
This parameter is used for setting the speed of the
serial link between the instrument and a PC.
Baud rate
Option: 0 to 9999.
This option is used to set the instrument’s ID. The
instrument ID is used while exchanging data with a
PC.
Instrument ID
Press Modify to access the instrument ID screen.
Press ARROW in order to select the desired value
between 0 and 9999. Press Accept to confirm the
value or ESC to return to the setup menu without
saving the new value.
Press the function key to select the new option. When
enabled, a short beep is heard every time a key is
pressed or when the calibration can be confirmed.
A long beep alert sounds when the pressed key is
not active or a wrong condition is detected while in
calibration.
Beep On

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Press Select to view the firmware version, language
version, mV factory calibration date and time and
temperature factory calibration date and time.
Press ESC to return to the Setup mode.
Meter information

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ELECTRODE PREPARATION
PREPARATION PROCEDURE
Remove the electrode protective cap.
DO NOT BE ALARMED IF ANY SALT DEPOSITS ARE PRESENT. This is normal with electrodes and
they will disappear when rinsed with water.
During transport tiny bubbles of air may have formed inside the glass bulb. The electrode cannot
function properly under these conditions. These bubbles can be removed by "shaking down" the
electrode as you would do with a glass thermometer.
If the bulb is dry, soak the electrode in HI 70300 Storage Solution for at least one hour.
Preparation of reference electrode:
• Unwrap ParafilmTM seal found over ceramic junction
on inner stern of the reference electrod and discard.
This is only used for shipping.
• Rinse inner stern with deionized water making cer-
tain to wet o-ring found on the inner stern.
• Reassemble reference electrode by gently pushing
the inner assembly into the outer body, sliding spring
down cable, and screwing cap into place.
• Remove fill hole cover and o-ring or fill hole spout.
• Using the dropper pipette provided, add a few drops of HI
7072 filling solution to the reference electrode, wetting
the o-ring and rinsing out the fill solution chamber.
• Holding the body of the electrode depress the black cap with your thumb. This permits the
fill solution to drain out of the body. Verify if the electrode returns to its original position.
(You may need to gently assist for this to occur).
• Tighten the electrode cap onto the body and fill electrode body with HI 7072 filling solution
until fill solution volume is just below fill hole.
Note: During measurement always operate reference electrode with the fill hole open.
MEASUREMENT
Place pH electrode and reference electrode into electrode holder and connect the Cable
Connectors to the instrument.
Rinse the pH and reference electrodes tip with distilled water. Immerse the pH and reference
electrodes (bottom 4 cm /1½”) in the sample and stir gently for a few seconds.
For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip
with a few drops of the solution to be tested, before taking measurements.

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ELECTRODE CALIBRATION PROCEDURE
It is recommended to calibrate the instrument frequently, especially if high accuracy is required.
The pH range should be recalibrated:
a) Whenever the pH electrode is replaced
b) At least once a week
c) After testing aggresive chemicals and after electrode is cleaned
d) When extreme accuracy is required
e) If the pH calibration expired warning is displayed during measurement. Every time
you calibrate the instrument use fresh buffers and perform an electrode Cleaning Procedure (see
page 45).
PROCEDURE
A one, two or three-point calibration can be performed, using 3 buffers (4.01, 6.00 and 8.30 pH).
Note: The HI 84429 will not accept other pH buffers for calibration.
• Pour small quantities of selected buffer solutions into clean beakers. For accurate calibration use
two beakers for each buffer solution, the first one for rinsing the electrode and the second one
for calibration.
• Put a magnetic stir bar in each beaker with the calibration buffer solution.
•
Remove the protective cap and rinse the electrodes with some of the buffer solution to be used
for the first calibration point.
• Put the first beaker with calibration buffer in the beaker holder.
• Place the electrode holder on the top of the beaker and secure it by turning clockwise.
•
Immerse the pH and reference electrodes and the temperature probe approximately 2 cm
(0.8”) into the buffer paying attention not to touch the stir bar.
To select Electrode calibration screen follow the next steps:
• From pH meter screen press CAL function key then Electrode.
• From Titrator screen press CAL function key then Electrode.

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THREE POINT CALIBRATION
• From the pH meter/titrator calibration menu screen select the Electrode calibration option.
The electrode calibration screen will be displayed.
• The 6.00 buffer will be selected by default. If necessary press the ARROW keys in order to
select a different buffer value.
• The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
• When the reading is stable and close to the selected buffer, the (unstable measurement)
symbol will disappear and the CFM key will become active.
• Press CFM to confirm the calibration.
• The calibrated value will be shown on the display and the second expected buffer value will be
displayed.
• After the first calibration point has been confirmed, press STIR to stop stirring.
• Remove the electrode holder with electrodes from the top of the beaker.
• Place the second buffer into beaker and place in beaker holder. Rinse the electrodes in a beaker
containing the second buffer rinsing solution.
• Place the electrode holder (with electrodes) on the top of the beaker, lock cap by turning and
press STIR.
• If necessary press the ARROW keys in order to select a different buffer value.
• The (unstable measurement) symbol will be shown on the display until the reading becomes stable.