Hanna Instruments HI 96813 User manual

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HI96813
Refractometer for Sucrose
in Wine and Grape Products
Measurements
INSTRUCTION MANUAL

All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary informa‑
tion for correct use of this instrument, as well as a precise
idea of its versatility.
If you need additional technical information, do not
hesitate to e‑mail us at [email protected] or view our
worldwide contact list at www.hannainst.com.
Dear
Customer,

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Preliminary Examination ........................................................
General Description................................................................
Specifications........................................................................
Principle of Operation............................................................
Functional Description............................................................
Display Elements...................................................................
Measurement Guidelines........................................................
Calibration Procedure.............................................................
Measurement Procedure.........................................................
To Change Measurement Unit.................................................
To Change Temperature Unit and Conversion Factor.................
Making a Standard % Brix Solution.......................................
Battery Replacement.............................................................
Error messages...........................................................................
Accessories............................................................................
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TABLE OF CONTENTS
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Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96813 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
PRELIMINARY EXAMINATION

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The HI96813 Digital Wine Refractometer is rugged portable, water resistant
devices that benefit from Hanna Instruments’ years of experience as a manu‑
facturer of analytical instruments. Hanna Instruments offers five related wine
refractometers to fill the differing requirements found in the industry. All are
equally valid ways to measure the sugar content of grape or must samples
in the field or winery. The HI96813 are optical instruments that are based
on the measurement of the refractive index of a solution. The measurement
of refractive index is simple and quick and provides the vintner a standard
accepted method for sugar content analysis. Samples are measured after a
simple user calibration with deionized or distilled water. Within seconds the
instrument measures the refractive index of the grape. These digital refrac‑
tometers eliminate the uncertainty associated with mechanical refractometers
and are easily portable for measurements in the field. Temperature (in °C
or °F) is displayed simultaneously with the measurement on the large dual
level display along with icons for Low Power and other helpful message codes.
Key features include:
• Dual‑level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non‑use
• Single‑point calibration with distilled or deionized water
• Waterproof models offers IP65 waterproof protection
• Quick, precise results readings are displayed in approximately 1.5 seconds
• Small sample size as small as 2 metric drops.
GENERAL DESCRIPTION

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Range
0.0 to 50.0 %Brix / 0.0 to 25.0 %
Potential Alcohol / 0.0 to 80.0 ºC
(32.0 to 176.0 ºF)
Resolution 0.1 %Brix / 0.1 % V/V Potential Alcohol /
0.1 ºC (0.1 ºF)
Accuracy ±0.2 %Brix / ±0.2% V/V Potential
Alcohol / ±0.3 ºC (±0.5 ºF)
Temperature
Compensation
Automatic between 10.0 and 40.0 ºC
(50.0 ‑ 104.0 ºF)
Measurement Time Approximately 1.5 seconds
Minimum Sample
Volume 100 µL (cover prism totally)
Light Source Yellow Led
Sample Cell Stainless Steel ring and flint glass prism
Case Material ABS
Enclosure Rating IP65
Battery Type / Life 9V/ 5000 readings
Auto‑Off After 3 minutes of non‑use
Dimensions 192 x 102 x 69 mm (7.6 x 4.1 x 2.7")
Weight 350 g (12.3 oz.)
SPECIFICATIONS
The %Brix determination is made by measuring the refractive index of a
solution. Refractive Index is an optical characteristic of a substance and the
number of dissolved particles in it. Refractive Index is defined as the ratio of
the speed of light in empty space to the speed of light in the substance. A
result of this property is that light will “bend”, or change direction, when it
travelsthroughasubstanceofdifferentrefractiveindex.Thisiscalledrefraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2/ n1
PRINCIPLE OF OPERATION

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HI96813 convert the refractive index of the sample to sucrose concentration
in units of percent by weight, %Brix (also referred to as °Brix). The conversion
used is based on the ICUMSA Methods Book (International Commission for
Uniform Methods of Sugar Analysis). Since the majority of sugar in grape
juice is fructose and glucose and not sucrose, the reading is sometimes
referred to as “Apparent Brix”. The HI96813 allows the user to tailor the
instrument to their specific needs based on their experience, since no fixed
conversion factor is universally applicable. The first conversion is based on
the %Brix value and an adjustable conversion factor between 0.50 and 0.70
(0.55 is a common value).
Potential alcohol (%V/V) = (0.50 to 0.70) x %Brix
One drawback of the above equation is that it does not take into account the
nonfermentable sugars and extract.
A second equation was also added that takes these factors into account and
can give a more accurate estimate of the alcohol content in the finished wine.
This conversion is named “C1” on the meter, and uses the following equation:
Potential Alcohol (%V/V) = 0.059 x [(2.66 x °Oe) ‑ 30] (C1)
Wheren2istherefractiveindexofthelower‑densitymedium;n1istherefractive
index of the higher‑density medium.
In the HI96813, light from an LED passes through a prism in contact with
the sample. An image sensor determines the critical angle at which the light
is no longer refracted through the sample.
PRINCIPLE OF OPERATION

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1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. RANGE Key (User Measurement Unit)
7. ON/OFF
8. Stainless Steel Sample Well and Prism
TOP VIEW
FUNCTIONAL DESCRIPTION
9. Battery Cover
10. Battery Compartment
BOTTOM
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1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Unit
7. Automatic Temperature Compensation
(blinks whentemperature exceeds 10.0‑40.0 °C / 50.0‑104.0 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when
blinking, temperature has exceeded operation range: 0.0‑80.0 °C /
32.0‑176.0 °F)
DISPLAY ELEMENTS

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• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• The instrument is intended to measure Wine and Grape products. Do not
expose instrument or prism to solvents that will damage it. This includes
most organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
MEASUREMENT GUIDELINES