Hanna Instruments HI 95715 User manual

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www.hannainst.com This Instrument is in
Compliance with the CE Directives
Instruction Manual
HI 95715
Ammonia Medium Range ISM

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TABLE OF CONTENTS
PRELIMINARY EXAMINATION ..............................................................................................3
GENERAL DESCRIPTION ......................................................................................................4
ABBREVIATIONS ................................................................................................................4
SPECIFICATIONS ................................................................................................................5
PRECISION AND ACCURACY.................................................................................................5
PRINCIPLE OF OPERATION..................................................................................................6
FUNCTIONAL DESCRIPTION.................................................................................................7
GUIDE TO DISPLAY CODES..................................................................................................9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT.............................................................11
MEASUREMENT PROCEDURE ............................................................................................13
VALIDATION PROCEDURE..................................................................................................15
CALIBRATION PROCEDURE................................................................................................16
BATTERY REPLACEMENT ...................................................................................................18
ACCESSORIES .................................................................................................................18
CE DECLARATION OF CONFORMITY....................................................................................19
WARRANTY .................................................................................................................19
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at [email protected].
This instrument is in compliance with directives.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

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PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 95715 Ion Selective Meter is supplied complete with:
• Two Sample Cuvets and Caps
• 9V Battery
• Tissue for wiping cuvets
• Instruction Manual and Quick Reference Guide
• Instrument Quality Certificate
• Rigid carrying case
Note:save all packing material until you are sure that the instrument works correctly.
Any defective item must be returned in its original packing.

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The HI 95715 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on a LED light source and a narrow band interference filter that allows most
accurate and repeatable readings. All instruments are factory calibrated and the electronic and
optical design minimizes the need of frequent calibration.
With the powerful CAL CHECK validation function you are able to validate good performance of
your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna ready-made, NIST traceable standards to verify the performance of the
instrument and recalibrate if necessary.
All instruments are splash proof and the LED (Light Emitting Diode) and filter units are protected
from dust or dirt by a transparent cup. This makes the instruments fulfill field applications.
Display codes aid the user in routine operation. The meter has an auto-shut off feature that will
turn off the instrument after 10 minutes of non use or after 1 hour if left in the calibration mode.
The meter uses an exclusive positive-locking system to ensure that the cuvet is in the same
positionevery timeit isplaced intothe measurementcell. Itis designedto fita cuvetwith alarger
neck making it easier to add both sample and reagents. The cuvet is made from special optical
glass to obtain best results.
The HI 95715 meter measures the ammonia nitrogen (NH3-N) content in water samples in the
0.00 to 9.99 mg/L (ppm) range. The method is an adaptation from ASTM Manual of Water and
Environmental Technology, D1426-93, Nessler Method.
The reagents are in liquid form and are supplied in bottles. The amount of reagent is precisely
dosed to ensure the maximum repeatability.
GENERAL DESCRIPTION
ABBREVIATIONS
American Society for Testing and Materials
degree Celsius
degree Fahrenheit
Liquid Crystal Display
Light Emitting Diode
milligrams per liter. mg/L is equivalent to ppm (part per million)
milliliter
ASTM:
°C:
°F:
LCD:
LED:
mg/L:
mL:

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Range 0.00 to 9.99 mg/L (as NH3-N)
Resolution 0.01 mg/L for measurement
0.1 mg/L for calibration and validation
Precision ±0.12 mg/L @ 6.00 mg/L
Typical EMC Deviation ±0.01 mg/L
Light Source Light Emitting Diode with narrow band interference filter @ 466 nm
Light Detector Silicon Photocell
Method AdaptationoftheASTMManualofWaterandEnvironmentalTechnology,
D1426-93, Nessler Method. The reaction between ammonia and the
reagents causes a yellow tint in the sample.
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off After 10' of non-use in measurement mode;
after 1 hour of non-use in calibration mode.
Dimensions 180 x 83 x 46 mm (7.1 x 3.3 x 1.8")
Weight 290 g (10 oz.).
REQUIRED REAGENTS
Code Description Quantity/test
HI 93715A-0 First Reagent 4 drops (6 drops in seawater)
HI 93715B-0 Second Reagent 4 drops (10 drops in seawater)
SPECIFICATIONS
Precision is how closely repeated measurements
agreewitheachother. Precisionisusuallyexpressed
asstandard deviation (SD).
Accuracyisdefinedasthenearnessofatestresultto
the true value.
Although good precision suggests good accuracy,
preciseresultscanbeinaccurate.Thefigureexplains
these definitions.
In a laboratory using a standard solution of 6.00
mg/L ammonia nitrogen (NH3-N) and a
representativelotofreagents,anoperatorobtained
with a single instrument a standard deviation of
0.12 mg/L.
PRECISION AND ACCURACY

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Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter.When a light beamcrosses a substance,some of theradiation may be absorbedby atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law: -log I/Io= ελc d
or
A = ελc d
Where:
-log I/Io= Absorbance (A)
Io= intensity of incident light beam
I= intensity of light beam after absorption
ελ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from
a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 95715 colorimeter is based on a Light Emitting Diode (LED)
combined with a narrow-band interference filter to guarantee both high performance and reliable
results.
HI 95715 block diagram (optical layout)
PRINCIPLE OF OPERATION

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A microprocessor controlled LED emits radiation which is first optically conditioned and beamed
to the sample contained in the cuvet. The optical path is fixed by the diameter of the cuvet. Then
the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity
Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
Themeasurementprocess iscarriedoutin twophases:first themeteriszeroed andthenthe actual
measurement is performed.
The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvetfor both. Itisnecessary that thesurface of thecuvet is cleanand not scratched. Thisto avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.
FUNCTIONAL DESCRIPTION
1) Liquid Crystal Display (LCD)
2) Cuvet Holder
3) Cuvet alignment indicator
4) ON/OFF key
5) ZERO key
6) READ/TIMER key
7) CAL CHECK key

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KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off.
• ZERO: to zero the meter prior to measurement.
• READ/TIMER: this is a bi-functional key, just press for making a measurement, or hold the
key for 3 seconds to start a pre-programmed countdown prior to measurement.
• CAL CHECK: this bi-functional key allows to perform validation of the instrument just pressing
the CAL CHECK key, or hold the key for 3 seconds to enter the calibration mode.
OPERATING MODES
•Measurement mode: default operation mode, enables both validation and measurement.
•Calibration mode: may be entered by keeping CAL CHECK pressed for three seconds (the “CAL”
icon appears), it enables calibration of the instrument.
DISPLAY ELEMENTS DESCRIPTION
1) four digit main display area.
2) CAL: appears when the instrument operates in the calibration mode.
3) the Month and Day icons appear above the number of month and day when a date is
displayed.
4) the blinking “Battery” icon appears when the battery voltage is getting low.

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This prompt appears for 1 second each time the instrument is
turned on.
This indicates that the meter is in a ready state and zeroing can
be performed.
Sampling in Progress. This flashing prompt appears each time the
meter is performing a measurement.
Thisindicates thatthemeter isin azeroed stateandmeasurement
can be performed.
Light over range: the cuvet is not inserted correctly and an excess
ambient light is reaching the detector. If the cover is properly
installed, then contact your dealer or the nearest Hanna Customer
Service Center.
Theblinking "BAT" indicatesthat thebattery voltage isgetting low
and the battery needs to be replaced.
This indicates that the battery is dead and must be replaced. Once
this indication is displayed, the meter will lock up. Change the
battery and restart.
“Configuration”: this indicates that the meter has lost its
configuration. Contact your dealer or the nearest Hanna Customer
Service Center.
CALIBRATION MODE MESSAGES
The date of the last calibration appears on the LCD each time the
calibration mode is entered. If calibration is performed for the first
time, “F.CAL” appears.
This indicates that the factory calibration is selected.
GUIDE TO DISPLAY CODES

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The flashing digits indicate that:
1) the month can be set
2) the day can be set
“Storage”: this prompt appears for 1 second at the end of the
calibration procedure, to indicate that the calibration data has
been stored.
“Error”: the concentration of the calibration solution used is not
correct. Repeat the calibration procedure with the right standard
solution,andverifyitisnotexpired.Ifthecalibrationprocedurefails
again, contact your dealer or the nearest Hanna Customer Service
Center.
ERROR MESSAGES
a) on zero reading
Thisindicatesthatthe zeroingprocedurefailed duetoalow signal-
to-noise ratio. In this case press ZERO again.
“NoLight”:theinstrumentcannotadjustthelightlevel.Pleasecheck
thatthesampledoesnotcontainanydebris.
“Light Low”: there is not enough light to perform a measurement.
Pleasecheckthe preparationofthezero cuvet.
“Light High”: there is too much light to perform a measurement.
Pleasecheckthe preparationofthezero cuvet.
b) on sample reading
There is too much light for the sample measurement. Please check
if the right sample cuvet is inserted.
“Inverted”:the sample and thezero cuvet are inverted.
“Zero”: a zero reading was not taken. Follow the instruction in
the measurement procedure for zeroing the meter.

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Under range. A blinking "0.00" indicates that the sample absorbs
less light than the zero reference. Check the procedure and make
sureyouusethesamecuvetforreference(zero)andmeasurement.
A flashing value of the maximum concentration indicates an
over range condition. The concentration of the sample is beyond
the programmed range: dilute the sample and re-run the test.
A flashing value lower than the maximum concentration indicates
a low signal-to-noise ratio condition. In this case accuracy of the
result is not guaranteed. Repeat the measurement procedure.
The instructions listed below should be carefully followed during testing to ensure best accuracy.
• Color or suspended matter in large amounts may
cause interference, therefore these should be
removed by treatment with active carbon and by
prior filtration.
• For a correct filling of the cuvet: the liquid in
the cuvet forms a convexity on the top; the
bottom of this convexity must be at the same
level of the 10 mL mark.
• Proper use of the dropper:
(a) to get good reproducible results, tap the dropper on the table for several times and wipe
the outside of the dropper tip with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
(a) (b)

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• It is important that the sample does not contain
any debris. This would corrupt the reading.
• In order to avoid reagent leaking and to obtain
more accurate measurements, it s recommended
to close the cuvet first with the supplied HDPE
plastic stopper and then with the black cap.
• Each time the cuvet is used, the cap must be
tightened to the same degree.
• Wheneverthecuvetisplacedintothemeasurement
cell, it must be dry outside, and completely free
of fingerprints, oil or dirt. Wipe it thoroughly with
HI 731318 (tissue for wiping cuvets, see chapter
ACCESSORIES)oralint-freeclothpriortoinsertion.
• Do not let the reacted sample stand too long after
reagent is added, or accuracy will be lost.
• It is possible to take multiple readings in a row,
but it is recommended to take a new zero reading
for each sample and to use the same cuvet for
zeroing and measurement.
• After the reading it is important to discard
immediatelythesample, otherwisetheglass might
become permanently stained.
• All the reaction times reported in this manual are
referred to 20°C (68°F). As a general rule of
thumb, they should be doubled at 10°C (50°F)
and halved at 30°C (86°F).
• In order to maximize accuracy, prior to a
measurement follow the validation procedure
to be sure that the instrument is properly
calibrated. If necessary, calibrate the instrument.

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MEASUREMENT PROCEDURE
• Turn the meter on by pressing ON/OFF.
• When the LCD displays “715”, it is ready.
• Fill the cuvet with 10 mL of unreacted sample,
up to the mark, and replace the cap.
• Place the cuvet into the holder and ensure that
the notch on the cap is positioned securely into
the groove.
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show
“ -0.0- ”. The meter is now zeroed and ready
for measurement.
• Remove the cuvet, open it and add 4 drops of
HI 93715A-0 First Reagent (6 drops in case
of seawater analysis). Swirl the cuvet gently
to mix.
• Add 4 drops of HI 93715B-0 Second Reagent
to the cuvet (10 drops in case of seawater
analysis). Replace the cap and swirl the cuvet
to mix.
MEASUREMENT PROCEDURE
Turn the meter on by pressing ON/OFF and follow instruction manual to perform either the
MEASUREMENT, VALIDATION or CALIBRATION PROCEDURE.

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• Replace the cuvet into the holder and ensure
that the notch on the cap is positioned securely
into the groove.
• Hold READ/TIMER for three seconds. The
display will show the countdown prior to
measurement.
Alternatively,waitfor3minutesand30seconds
and just press READ/TIMER.
In both cases “SIP” will blink during
measurement.
• The instrument directly displays concentration
in mg/L of ammonia nitrogen (NH3-N) on the
Liquid Crystal Display.
• To convert the reading to mg/L of ammonia
(NH3), multiply by a factor of 1.216.
or
INTERFERENCES
•Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine,
(positive error).
To eliminate these interferences distillation is required.
•Organic compounds like: aldehydes, alcohols (e.g. ethanol) or acetone (negative error).
To eliminate these interferences distillation is required.
•Sulfide (S2-): may cause turbidity.
•Hardness above 1 g/L as Calcium Carbonate (CaCO3).

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VALIDATION PROCEDURE
• Turn the meter on by pressing ON/OFF.
When the LCD displays “715”, it is ready.
• Place the CAL CHECK Standard Cuvet Ainto the
holder and ensure that the notch on the cap is
positioned securely into the groove.
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
validation.
• Remove the cuvet and place the CAL CHECK
StandardHI95715-11CuvetBintothe holder.
Ensure that the notch on the cap is positioned
securely into the groove.
• Press CAL CHECK and “SIP” will blink during
measurement.
• Wait for a few seconds and the display will
show the validation standard value.
The reading should be within specifications as
reported on the Cal Check Standard Certificate.
If the value found is out of specifications,
please check that the cuvets are free of
fingerprints, oil or dirt and repeat validation. If
results are still found out of specifications then
recalibrate the instrument.
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning:donot validateorcalibratethe instrumentwithstandardsolutions otherthantheHanna
CAL CHECK Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).

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CALIBRATION PROCEDURE
• Turn the meter on by pressing ON/OFF.
• When the LCD displays “715”, it is ready.
• EnterthecalibrationmodebyholdingCALCHECK
for three seconds.
• The date of the last calibration appears (e.g.:
month “01”, day “08”). “F.CAL” means that
the factory calibration is selected.
Note: at this point it is possible to reset the
instrument to restore FACTORY CALIBRATION,
see instructions page 18.
• Place the CAL CHECK Standard Cuvet Ainto the
holder and ensure that the notch on the cap is
positioned securely into the groove.
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
calibration.
• Remove the cuvet.
• Place the CAL CHECK Standard HI 95715-11
Cuvet Binto the holder and ensure that the
notch on the cap is positioned securely into the
groove.
CALIBRATION PROCEDURE
Note:it is possible to interrupt calibration procedure at any time by pressing ON/OFF.
Warning:donotvalidateorcalibratetheinstrumentwith standardsolutions otherthantheHanna
CAL CHECK Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).
or

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or
• Press READ/TIMER and “SIP” will blink during
measurement.
• The instrument will show for three seconds the
Cal Check standard value.
Thenthedateoflastcalibration(e.g.:“01.08”)
appearsonthedisplay,or“01.01”ifthefactory
calibration was selected. In both cases the
number of the month is blinking, ready for
date input.
Note:ifthedisplayshows“ERR”thecalibration
procedure failed. Verify that the CAL CHECK
Standards HI 95715-11 Cuvets, Aand B, are
free from fingerprints or dirt and that they are
inserted correctly.
DATE INPUT
• Keep READ/TIMER pressed to scroll to the
desired month number (01-12).
• When the correct month has been set, press
ZERO to confirm. Now the display will show the
daynumberblinking.KeepREAD/TIMERpressed
to scroll to the desired day number (01-31).
Note: it is possible to change from day to
month and vice versa by pressing ZERO.
• When both the day and month have been
selected, hold CAL CHECK for three seconds to
storedateandcalibrationvalues.Theinstrument
will show for one second “Stor”, to confirm
thatthenewcalibrationdatahasbeenaccepted.
• The instrument will return automatically to the
measurement mode by displaying "715" on
the LCD.

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REAGENT SETS
HI 93715-01 Reagents for 100 tests
HI 93715-03 Reagents for 300 tests
OTHER ACCESSORIES
HI 95715-11 CAL CHECK Standard Cuvets (1 set)
HI 710009 Blue rubber boot
HI 710010 Orange rubber boot
HI 721310 9V battery (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731331 Glass cuvets (4 pcs)
HI 731335 Caps for cuvets (4 pcs)
HI 93703-50 Cuvets cleaning solution (230 mL)
ACCESSORIES
Battery replacement must only take place in a
non-hazardous area.
Simply slide off the battery cover on the back of
the meter. Detach the battery from the terminals
andattachafresh9Vbatterywhilepayingattention
tothecorrectpolarity.Insertthebatteryandreplace
the cover.
9V
SLID
E
OFF
BATTERY REPLACEMENT
FACTORYCALIBRATIONRESET
It is possible to restore factory calibration:
• EnterthecalibrationmodebyholdingCALCHECK
for three seconds.
• Hold ZERO for 10 seconds. The display will
showfor2seconds“F.CAL”and"715"appears.
The factory calibration is automatically restored
and the instrument is ready for measurement.

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Recommendations for Users
Before using these products, make sure that they are
entirely suitable for your specific application and for
the environment in which they are used.
Operation of these instruments may cause
unacceptable interferences to other electronic
equipments, this requiring the operator to take all
necessary steps to correct interferences.
Any variation introduced by the user to the supplied
equipment may degrade the instruments' EMC
performance.
Toavoiddamagesorburns,donotput theinstrument
inmicrowaveoven.Foryoursandtheinstrumentsafety
do not use or store the instrument in hazardous
environments.
HI 95715 is warranted for two years against defects in workmanship and materials when used
for its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
IftheinstrumentistobereturnedtoHannaInstruments,firstobtainaReturnedGoodsAuthorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
CE DECLARATION OF CONFORMITY
WARRANTY
Hanna Instruments reserves the right to modify the design, construction and appearance of its
products without advance notice.

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MAN95715R1 09/03
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