Hanna Instruments HI 96769C User manual

INSTRUCTION MANUAL
HI96769C
Anionic Surfactants ISM

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of
the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Dear
Customer,

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PRELIMINARY EXAMINATION .............................................................................................
GENERAL DESCRIPTION .....................................................................................................
ABBREVIATIONS ...............................................................................................................
SPECIFICATIONS ...............................................................................................................
PRECISION AND ACCURACY ...............................................................................................
PRINCIPLE OF OPERATION ................................................................................................
FUNCTIONAL DESCRIPTION ...............................................................................................
ERRORS AND WARNINGS ..................................................................................................
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ..............................................................
STARTUP ..........................................................................................................................
MEASUREMENT PROCEDURE .............................................................................................
VALIDATION PROCEDURE ...................................................................................................
CALIBRATION PROCEDURE .................................................................................................
GLP .................................................................................................................................
BATTERY MANAGEMENT ....................................................................................................
BATTERY REPLACEMENT ....................................................................................................
ACCESSORIES ...................................................................................................................
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TABLE OF CONTENTS

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Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occurred during shipment, please contact your local Hanna Instruments Office.
Each HI96769 Ion Selective Meter is supplied complete with:
• Sample Cuvettes and Caps (2 pcs.)
• CAL Check™ Standard Cuvettes
• One long glass vial (25 mL) with cap
• Three plastic pipettes
• 9V Battery
• Cloth for wiping cuvettes
• Instrument Quality Certificate
• Instruction Manual and Quick Reference Guide
• Rigid carrying case
Note: Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
PRELIMINARY EXAMINATION

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The HI96769 is an auto diagnostic portable microprocessor meter that benefits from
Hanna Instruments’ years of experience as a manufacturer of analytical instruments. It has an
advanced optical system based on a special tungsten lamp and a narrow band interference filter
that allows most accurate and repeatable readings. All instruments are factory calibrated and the
electronic and optical design minimizes the need of frequent calibration.
With the powerful CAL CheckTM validation function, you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna Instruments ready‑made, NIST traceable standards to verify the performance of
the instrument and recalibrate if necessary.
All instruments are splash proof and the lamp and filter units are protected from dust or dirt by
a transparent cup. This makes the instruments fulfill field applications. Display messages aid the
user in routine operations. The meter has an auto‑shut off feature that will turn off the instrument
after 10 minutes of non use in measurement mode or after 1 hour if left in calibration mode.
The meter uses an exclusive positive‑locking system to ensure that the cuvette is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvette with a
larger neck making it easier to add both sample and reagents. The cuvette is made from special
optical glass to obtain best results.
The HI96769 meter measures the Anionic surfactants in the 0.00 to 3.50 mg/L (ppm) range, in
drinking, surface and waste waters. The method is an adaptation of the USEPA method 425.1
and Standard Methods for the Examination of Water and Wastewater, 20th edition, 5540C, Anionic
Surfactants as MBAS.
The reagent is in liquid form and is supplied in bottles. The amount of reagent is precisely dosed
to ensure the maximum repeatability.
degree Celsius
degree Fahrenheit
US Environmental Protection Agency
milligrams per liter. mg/L is equivalent to ppm (parts per million)
milliliter
millivolt
Liquid Crystal Display
Sodium Dodecyl Benzene Sulfonate
°C:
°F:
USEPA:
mg/L:
mL:
mV:
LCD:
SDBS:
GENERAL DESCRIPTION ABBREVIATIONS

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SPECIFICATIONS
Range 0.00 to 3.50 mg/L (as SDBS)
Resolution 0.01 mg/L
Accuracy
@25 °C (77 °F)
±0.04 mg/L ±3 % of reading
Light Source Tungsten lamp
Light Detector Silicon Photocell with narrow band interference filter @610 nm
Method
Adaptation of the USEPA method 425.1 and Standard Methods for the
Examination of Water and Wastewater, 20th edition, 5540C, Anionic Sur‑
factants as MBAS.
Environment 0 to 50 °C (32 to 122 °F)
max 95 % RH non‑condensing
Battery Type 9V (1 pc.)
Auto‑Shut off After 10’ of non‑use in measurement mode; after 1 hour of non‑use in
calibration mode; with last reading reminder
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
Weight 320 g (11.3 oz.)
REQUIRED REAGENTS
Code Description Quantity/test
HI95769A‑0 Anionic Surfactants Reagent A 4 drops
HI95769B‑0 Anionic Surfactants Reagent B 2 drops
Chloroform 10 mL
Dionized Water 15 mL

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Precision is how closely repeated measurements
agree with each other. Precision is usually expressed
as standard deviation (SD).
Accuracy is defined as the nearness of a test result
to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure explains
these definitions.
In laboratory using a standard solution of 1.00 mg/L
SDBS and a representative lot of reagents, an operator
obtained with a single instrument a standard deviation
of 0.04 mg/L.
PRECISION AND ACCURACY
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical‑chemical characteristics of the substance according to the
Lambert‑Beer Law:
‑log I/Io= ελc d
or
A = ελc d
Where:
‑log I/Io = Absorbance (A)
Io = intensity of incident light beam
I = intensity of light beam after absorption
ε
λ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
PRINCIPLE OF OPERATION

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A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of
the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light
beam of intensity Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by the sample and converts it
into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires particular
attention. It is important that both, the measurement and the calibration (zeroing) cuvettes, are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvette for both. It is necessary that the surface of the cuvette is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases,
it is necessary to close the cuvette to prevent any contamination.
Therefore, the concentration “c” can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from a
specific chemical reaction between sample and reagents. Given that the absorption of a compound
strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should
be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna Instruments’ HI96 series colorimeters is based on special subminiature
tungsten lamps and narrow‑band interference filters to guarantee both high performance and
reliable results.
HI96 series block diagram (optical layout)
PRINCIPLE OF OPERATION

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1) GLP/ key
2) CAL CHECK key
3) ZERO/CFM key
4) READ /TIMER key
5) ON/OFF key
6) Liquid Crystal Display (LCD)
7) Cuvette alignment indicator
8) Cuvette holder
INSTRUMENT DESCRIPTION
FUNCTIONAL DESCRIPTION
KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off.
• ZERO/CFM: to zero the meter prior to measurement, to confirm edited values or to confirm
factory calibration restore.
• READ /TIMER: this is a multi‑functional key. In measurement mode, press to make a
measurement, or press and hold for three seconds to start a pre‑programmed countdown prior
to measurement. In GLP mode press to view the next screen.
• CAL CHECK: this is a bi‑functional key. Just press to perform the validation of the meter, or
press and hold for three seconds to enter calibration mode.
• GLP/: this is a bi‑functional key. Just press to enter GLP mode. In calibration mode press
to edit the date and time.
OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed for three seconds (the
“CAL” tag appears), it enables calibration of the instrument.
• GLP mode: may be entered by pressing GLP/(“GLP” appears), it enables consulting of
user calibration date or restore factory calibration.

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DISPLAY ELEMENTS DESCRIPTION
1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon shows the charge state of the battery
4) The hourglass appears when an internal check is in progress
5) Status messages
6) The chronometer appears when the reaction timer is running
7) The month, day and date icons appear when a date is displayed
8) Four digit main display
9) Measuring units
10) Four digit secondary display
FUNCTIONAL DESCRIPTION

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The instrument shows clear messages when erroneous condition appears. Messages are also displayed
when the obtained values are outside expected range. The beeper is playing a beep on errors.
a) on zero reading
Light High: There is too much light to perform a measurement.
Please check the preparation of the zero cuvette.
Light Low: There is not enough light to perform a measurement.
Please check the preparation of the zero cuvette.
No Light: The instrument cannot adjust the light level. Please check
that the sample does not contain any debris.
Inverted Cuvettes: The sample and the zero cuvette are inverted.
Zero: A zero reading was not taken. Follow the instructions of the
measurement procedure for zeroing the meter.
b) on sample reading
ERRORS AND WARNINGS
Under Range: A blinking “0.00” indicates that the sample absorbs
less light than the zero reference. Check the procedure and make sure
you use the same cuvette for reference (zero) and measurement.

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Over Range: A flashing value of the maximum concentration
indicates an over range condition. The concentration of the sample
is beyond the programmed range: dilute the sample and re‑run
the test.
c) during calibration procedure
Standard Low: The standard reading is less than expected.
Standard High: The standard reading is higher than expected.
Cap Error: Appears when external light enters in the analysis cell.
Assure that the cuvette cap is present.
Cooling Lamp: The instrument waits for the lamp to cool down.
d) other errors and warnings
Battery Low: The battery must be replaced soon.
Dead Battery: This indicates that the battery is dead and must
be replaced. Once this indication is displayed, normal operation
of the meter will be interrupted. Change the battery and restart
the meter.
ERRORS AND WARNINGS

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The instructions listed below should be carefully followed during testing to ensure best accuracy.
• For adding the exact amount of sample and deionized water
to the cuvette it is strongly recommended to use two class
A laboratory pipettes.
• Alternatively, fill the cuvette up to the 10 mL mark: the
liquid in the cuvette forms a concavity on the top; the
bottom of this concavity must be at the same level of the
10 mL mark.
• Proper use of dropper:
(a) to get good reproducible results, tap the dropper on the table for several times and wipe
the outside of the dropper with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
• Color or suspended matter in large amounts may cause interference, therefore these should be
removed by treatment with active carbon and by prior filtration.
• It is important that the sample does not contain any debris. This would corrupt the reading.
• Each time the cuvette is used, the cap must be tightened to the same degree.
class A pipette
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
(a) (b)
• In order to avoid reagent leaking and to obtain more accurate
measurements, it is recommended to close the cuvette first
with the supplied HPDE plastic stopper and then with the black
cap.

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Prepare the instrument for measurement as follows:
• Unpack the instrument by removing the dust protection sleeve from the instrument cuvette
holder.
• Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.
• Place the instrument on a flat table.
• Do not place the instrument under direct sun light.
• Whenever the cuvette is placed into the measurement cell, it must be dry outside, and completely
free of fingerprints, oil or dirt. Wipe it thoroughly with HI731318 or a lint‑free cloth prior to
insertion.
• Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain
accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.
• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
• It is possible to take multiple readings in a row, but it is recommended to take a new zero
reading for each sample and to use the same cuvette for zeroing and measurement.
• After the reading it is important to discard immediately the sample, otherwise the glass might
become permanently stained.
• All the reaction times reported in this manual are referred to 25 °C (77 °F). In general, the
reaction time should be increased for temperatures lower than 20 °C (86 °F), and decreased
for temperatures higher than 25 °C (77 °F).
• In order to maximize accuracy, prior to a measurement follow the validation procedure to be
sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
STARTUP

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• Turn the meter on by pressing ON/OFF. The display briefly shows all tags on.
• When the beeper sounds briefly and the LCD displays dashes, the meter is ready. The blinking
“ZERO” indicates that the instrument needs to be zeroed first.
• Fill the graduated glass vial with 25 mL of sample.
For most accurate results, use of a class A laboratory pipette is strongly recommended.
Alternatively, it is possible to use the plastic pipette, to fill the glass vial up to the 25 mL mark.
• Add 2 drops of HI95769A‑0 Anionic Surfactants Reagent A and 2 drops of HI95769B‑0 Anionic
Surfactants Reagent B.
MEASUREMENT PROCEDURE

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Note: Chloroform is heavier than water thus it will sink to the bottom of the graduated glass
vial; Do not mix up the pipettes.
• Close tightly the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Block the cap with a finger during shaking.
• Press and hold READ /TIMER for three seconds and the display will show the countdown.
The beeper is playing a beep at the end of countdown period.
• Close the glass vial with its cap and invert to mix. The solution will turn blue.
• Add exactly 10 mL of Chloroform reagent. For most accurate results, use a class A glass laboratory
pipette is strongly recommended.
Alternatively, it is possible to use another clean plastic pipette to fill the vial up to the 10 mL
mark.
MEASUREMENT PROCEDURE
3’’

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Alternatively, wait for 2 minutes leaving the vial capped and undisturbed
During this period, the chloroform layer separates from the aqueous layer: the color of the
aqueous layer will fade slightly if anionic surfactants are present, while the chloroform layer
will turn blue.
• Remove the cap.
• Using the long plastic pipette, remove the upper aqueous layer and discard. Do not remove the
lower chloroform layer.
• Add to the vial about 15 mL of deionized water, up to the 25 mL mark.
• Add 2 drops of HI95769A‑0 Anionic Surfactants Reagent A.
MEASUREMENT PROCEDURE

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• Close tightly the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Block the cap with a finger during shaking.
• Press and hold READ /TIMER for three seconds and the display will show the countdown.
The beeper is playing a beep at the end of countdown period.
Alternatively, wait for 2 minutes leaving the vial capped and undisturbed
During this period, the chloroform layer separates from the aqueous layer.
• Remove the cap.
• Insert a clean plastic pipette below the upper aqueous layer to transfer only the lower chloroform
layer into a cuvette paying attention not to transfer the upper aqueous layer too.
• The solution in the cuvette must be limpid.
MEASUREMENT PROCEDURE
3’’

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• If the solution is clouded, you can improve separation between the chloroform and aqueous
layers by gently warming the cuvette (for instance by holding the cuvette in the hand).
• If the chloroform layer contains some aqueous drops hanging on the cuvette wall, gently swirl
or invert the cuvette.
• It is important to transfer at least 7 mL of chloroform layer into the measurement cuvette, thus
up to 0.5 cm (1/4”) below the 10 mL mark. If the transferred volume is lower than 7 mL, the
accuracy of the test may be affected. Please repeat the test waiting for longer than 2 minutes,
to allow complete separation between the two phases.
• Cap the cuvette. This is the reacted sample (#2).
• Fill another cuvette with 10 mL of Chloroform reagent up to the 10 mL mark and place the cap.
This is the blank (#1).
• Place the blank (cuvette #1) into the cuvette holder and ensure that the notch on the cap is
positioned securely into the groove.
MEASUREMENT PROCEDURE
#1

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• Press ZERO/CFM and the lamp, cuvette and detector icons will appear on the display, depending
on the measurement phase.
• After a few seconds, the display will show “-0.0-”. The meter is now zeroed and ready for
measurement.
• Remove the cuvette.
• Place the reacted sample (cuvette #2) into the cuvette holder and ensure that the notch on
the cap is positioned securely into the groove.
• Press READ /TIMER.The lamp, cuvette and detector icons will appear on the display,
depending on the measurement phase.
MEASUREMENT PROCEDURE
#2