Hanna Instruments HI 96750 User manual

1
Instruction Manual
HI 96750
Potassium
LR and MR ISM
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TABLE OF CONTENTS
PRELIMINARY EXAMINATION .............................................................................................. 3
GENERAL DESCRIPTION ......................................................................................................4
ABBREVIATIONS ................................................................................................................ 4
SPECIFICATIONS ................................................................................................................ 5
PRECISION AND ACCURACY ................................................................................................. 5
PRINCIPLE OF OPERATION .................................................................................................. 6
FUNCTIONAL DESCRIPTION ................................................................................................7
ERRORS AND WARNINGS ..................................................................................................9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ...........................................................11
STARTUP.........................................................................................................................12
RANGE SELECTION ...........................................................................................................13
MEASUREMENT PROCEDURE ...........................................................................................13
VALIDATION PROCEDURE .................................................................................................15
CALIBRATION PROCEDURE ...............................................................................................17
GLP .................................................................................................................................20
BATTERY MANAGEMENT ..................................................................................................21
BATTERY REPLACEMENT ..................................................................................................22
ACCESSORIES ...................................................................................................................22
WARRANTY......................................................................................................................23
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at [email protected].
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
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PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 96750 Ion Selective Meter is supplied complete with:
•Two Sample Cuvettes and Caps
•CAL CHECK standard cuvettes
•9V Battery
•Scissors
•1000 mL automatic pipette with tip
•Cloth for wiping cuvettes
•Instrument quality certificate
•Instruction Manual
•Rigid carrying case
Note:Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
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GENERAL DESCRIPTION
ABBREVIATIONS
The HI 96750 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on a special Light Emitting Diode (LED) and a narrow band interference filter that
allows most accurate and repeatable readings. All instruments are factory calibrated and the
electronic and optical design minimizes the need of frequent calibration.
With the powerful CAL CHECKTM validation function, you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive HANNA ready-made, NIST traceable standards to verify the performance of the
instrument and recalibrate if necessary.
All instruments are splash waterproof and the LED and filter units are protected from dust or dirt
by a transparent cup. This makes the instruments fulfill field applications. Display messages aid
the user in routine operation. The meter has an auto-shut off feature that will turn off the
instrument after 10 minutes of non use in measurement mode or after 1 hour if left in
calibration mode .
The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvette with a
larger neck making it easier to add both sample and reagents. The cuvette is made from special
optical glass to obtain best results.
The HI 96750 meter measures the potassium (K) content in water samples in the 0.0 to 10.0
mg/L (ppm) for low range and 10 to 100 mg/L (ppm) for medium range. Tetraphenylborate
Method. The reaction between potassium and reagents causes turbidity in the sample.
The reagents are in powder and liquid form and are supplied in packets and bottles respectively.
The amount of reagent is precisely dosed to ensure the maximum repeatability.
degree Celsius
degree Fahrenheit
milligrams per liter. mg/L is equivalent to ppm (parts per million)
milliliter
millivolts
°C:
°F:
mg/L:
mL:
mV:
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SPECIFICATIONS
PRECISION AND ACCURACY
Range 0.0 to 10.0 mg/L LR
10 to 100 mg/L MR
Resolution 0.1 mg/L LR
1 mg/L MR
Accuracy LR: ±1.5 mg/L ±7% of reading @ 25°C
MR: ±15 mg/L ±7% of reading @ 25°C
(excluding dilution error)
Typical EMC Deviation ±0.1 mg/L LR
±1 mg/L MR
Light Source Light Emitting Diode
Light Detector Silicon Photocell with narrow band interference filter @ 466nm
Method Tetraphenylborate Method. The reaction between potassium and re-
agents causes turbidity in the sample.
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off After 10' of non-use in measurement mode ;
after 1 hour of non-use in calibration mode ;
with last reading reminder.
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
Weight 360 g (12.7 oz.).
REQUIRED REAGENTS
Code Description Quantity/test
HI93750A-0 Potassium Reagent 6 drops
HI93750B-0 Powder Reagent 1 packet
PrecisionPrecision
PrecisionPrecision
Precision is how closely repeated measurements
agree with each other. Precision is usually
expressed as standard deviation (SD).
AccuracyAccuracy
AccuracyAccuracy
Accuracy is defined as the nearness of a test
result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure
explains these definitions.
In a laboratory using a standard solution of 2.00
mg/L potassium and a representative lot of
reagent, an operator obtained with a single
instrument a standard deviation of 0.06 mg/L.
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PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log I/Io= ελc d
or
A = ελc d
Where:
-log I/Io=Absorbance (A)
Io=intensity of incident light beam
I=intensity of light beam after absorption
ελ=molar extinction coefficient at wavelength λ
c=molar concentration of the substance
d=optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 96750 colorimeter is based on special LED and narrow-band
interference filters to guarantee both high performance and reliable results.
HI 96750 block diagram (optical layout)
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1) RANGE/GLP/ key
2) CAL CHECK key
3) ZERO/CFM key
4) READ/ /TIMER key
5) ON/OFF key
6) Liquid Crystal Display (LCD)
7) Cuvette alignment indicator
8) Cuvette holder
FUNCTIONAL DESCRIPTION
A microprocessor controlled special LED emits radiation which is first optically conditioned and
beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the
cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light
beam of intensity Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the
actual measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires
particular attention. It is important that both, the measurement and the calibration (zeroing)
cuvettes, are optically identical to provide the same measurement conditions. Whenever possible
use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not
scratched. This to avoid measurement interference due to unwanted reflection and absorption of
light. It is recommended not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvette to prevent any contamination.
INSTRUMENT DESCRIPTION
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KEYPAD DESCRIPTION
•ON/OFF: to turn the meter on and off.
•ZERO/CFM: to zero the meter prior to measurement, to confirm edited values or to confirm
factory calibration restore.
•READ/ /TIMER: this is a multi-functional key. In measurement mode , press to make a
measurement, or press and hold for three seconds to start a pre-programmed countdown prior
to measurement. In GLP mode press to view the next screen.
•CAL CHECK: this is a bi-functional key. Just press to perform the validation of the meter, or
press and hold for three seconds to enter calibration mode .
• RANGE/GLP/: this is a multi-functional key. Just press to enter GLP mode . In calibration
mode
press to edit the date and time.
OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed for three seconds (the
“CAL” tag appears), it enables calibration of the instrument.
• GLP mode may be entered by pressing RANGE/GLP/(“GLP” appears), it enables
consulting of user calibration date or restore factory calibration.
DISPLAY ELEMENTS DESCRIPTION
1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon shows the charging level of the battery
4) The hourglass appears when an internal checking is in progress
5) Status messages
6) The chronometer appears when the reaction timer is running
7) The month, day and date icons appear when a date is displayed
8) Four digit main display
9) Measuring units
10) Four digit secondary display
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ERRORS AND WARNINGS
The instrument shows clear messages when erroneous condition appears. Messages are also displayed
when the obtained values are outside expected range. The beeper is playing a beep on errors.
a) on zero reading
Light High: There is too much light to perform a measurement.
Please check the preparation of the zero cuvette.
Light Low: There is not enough light to perform a measurement.
Please check the preparation of the zero cuvette.
No Light:The instrument cannot adjust the light level. Please
check that the sample does not contain any debris.
Inverted cuvettes: The sample and the zero cuvette are inverted.
Zero: A zero reading was not taken. Follow the instructions of
the measurement procedure for zeroing the meter.
b) on sample reading
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Under range: A blinking “0.0” indicates that the sample
absorbs less light than the zero reference. Check the procedure
and make sure you use the same cuvette for reference (zero) and
measurement.
Over Range: A flashing value of the maximum concentration
indicates an over range condition. The concentration of the
sample is beyond the programmed range: dilute the sample and
re-run the test.
c) during calibration procedure
Standard Low: The standard reading is less than expected.
Standard High: The standard reading is higher than expected.
Cap error: Appears when external light enters in the analysis
cell. Assure that the cuvette cap is present.
Cooling lamp: The instrument waits for the lamp to cool down.
d) other errors and warnings
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The instructions listed below should be carefully followed during testing to ensure best accuracy.
•Color or suspended matter in large amounts may cause
interference, therefore these should be removed by
treatment with active carbon and by prior filtration.
•For a correct filling of the cuvette: the liquid in the
cuvette forms a concavity on the top; the bottom of this
concavity must be at the same level of the 10 mL mark.
•Proper use of the powder reagent packet:
(a) use scissors to open the powder packet;
(b) push the edges of the packet to form a spout;
(c) pour out the content of the packet.
•It is important that the sample does not contain any debris. This would corrupt the reading.
•Each time the cuvette is used, the cap must be tightened to the same degree.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
Battery low: The battery must be replaced soon.
Dead battery: This indicates that the battery is dead and must
be replaced. Once this indication is displayed, the meter will
lock up. Change the battery and restart the meter.
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•Whenever the cuvette is placed into the measurement cell, it
must be dry outside, and completely free of fingerprints, oil
or dirt. Wipe it thoroughly with HI 731318 or a lint-free
cloth prior to insertion.
•Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain
accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.
•Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
•It is possible to take multiple readings in a row, but it is recommended to take a new zero
reading for each sample and to use the same cuvette for zeroing and measurement.
•After the reading it is important to discard immediately the sample, otherwise the glass
might become permanently stained.
•All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule
of thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F).
•In order to maximize accuracy, prior to a measurement follow the validation procedure to
be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
•Proper use of dropper:
(a) to get good reproducible results, tap the dropper on the table for several times and wipe
the outside of the dropper with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
(a) (b)
STARTUP
Prepare the instrument for measurement as follows:
•Unpack the instrument by removing the dust protection sleeve from the instrument cuvette
holder.
•Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.
•Place the instrument on a flat table.
•Do not place the instrument under direct sun light.
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•Press RANGE/GLP/to change the range. The
range can be changed any time when the
instrument is in measurement mode .
The selected range is memorized and the selection
remaines unchanged at power off or when the
battery is removed.
MEASUREMENT PROCEDURE
To compensate the meter for the sample turbidity or color, the measurement takes place in two
phases. First, the meter is zeroed using the unreacted sample. After the reagents are added the
reacted sample is measured.
•Turn the meter on by pressing ON/OFF.
The display briefly shows all tags on.
•When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.
The blinking “ZERO” indicates that the
instrument needs to be zeroed first.
•Pay attention to the selected range.
To change the range, simply press
RANGE/GLP/.
The HI 96750 can measure Potassium LR when range P1 is selected, Potassium MR when range
P2 is selected. To change the active range follow the procedure:
•Turn the meter on by pressing ON/OFF. The
display briefly shows all tags on.
•After startup, the range identification number is
displayed on the secondary LCD as P1 or P2.
RANGE SELECTION
Code Parameter
P1 Potassium LR
P2 Potassium MR
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•Fill the cuvette with 10 mL of unreacted
sample, up to the mark, and replace the
cap.
•Add six drops of HI 93750A-0, replace
the cap and swirl the solution.
•Place the cuvette into the cuvette holder
and ensure that the notch on the cap is
positioned securely into the groove.
•Press ZERO/CFM and the lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
•After a few seconds, the display will
show “-0.0-”. The meter is now zeroed
and ready for measurement.
•Remove the cuvette.
•Add the content of one packet of
HI 93750B-0 powder reagent. Replace
the cap and shake gently for about 1
minute to dissolve most of the reagent.
•Replace the cuvette into the cuvette holder
and ensure that the notch on the cap is
positioned securely into the groove.
•Press and hold READ/ /TIMER for three
seconds. The display will show the
countdown prior to measurement. The
beeper is playing a beep at the end of
countdown period.
•Press and hold READ/ /TIMER for three
seconds. The display will show the
countdown prior to measurement. The
beeper is playing a beep at the end of
countdown period.
Alternatively, wait for 2 minutes then just
press READ/ /TIMER.
In both cases, the lamp, cuvette and
detector icons will appear on the display,
depending on the measurement phase.
•At the end of measurement, the instrument
directly displays concentration in mg/L of
potassium on the LCD.
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INTERFERENCES
The following ions do not interfere below the concentrations reported:
Ammonium 10 ppm
Calcium 10000 ppm as CaCO3
Chlorine 12000 ppm
Magnesium 8000 ppm as CaCO3
Sodium 8000 ppm
Color or suspended matter in large amounts will cause interference. Therefore the turbidity
should be removed by prior filtration.
For best results samples should be tested at room temperature (20OCC or 68OF). Lower
temperatures will result in higher readings.
VALIDATION PROCEDURE
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: Do not validate the instrument with any standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained.
Note: The validation is performed only for the selected parameter. For full validation of the
instrument, the following procedure must be performed for each parameter.
•If the analysis result is over the method range, the sample should
be diluted.
•Use the syringe to measure 10mL of sample.
•To measure exactly 10 mL of sample with the 5 mL syringe,
push the plunger completely into the syringe and insert the tip
into the sample. Pull the plunger out until the lower edge of the
seal is on the 5 mL mark of the syringe.
•Remove the cap and fill the Demineralizer Bottle with tap water.
•Replace the cap and shake gently for at least 2 minutes.
•Open the upper part of the Demineralizer Bottle cap and gently
squirt the demineralized water into the cylinder, up to the 100 mL
mark.
Note: The ion exchange resin contained in the Demineralizer Bottle
is provided with an indicator substance. The indicator will
change from green to blue when the resin has been exhausted
and needs to be replaced.
•Pour the solution in the 170 mL beaker, replace the cap and
invert several times to mix. This is the diluted sample.
•Select P2 program. The readings on diluted sample are performed
similary as for P1 method. The results are converted automatically
by meter.
Note: The conversion factor for dilution is included in software of P2.
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•Turn the meter on by pressing ON/OFF.
•When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.
Note:
•Place the CAL CHECKTM Standard
HI 96750-11 Cuvette A into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•Press ZERO/CFM and the lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
•After a few seconds, the display will
show “-0.0-”. The meter is now zeroed
and ready for validation.
•Remove the cuvette.
•Place the CAL CHECKTM Standard
HI 96750-11 Cuvette B into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•Press CAL CHECK and the lamp, cuvette
and detector icons together with “CAL
CHECK” will appear on the display,
depending on the measurement phase.
•At the end of the measurement the display
will show the validation standard value.
The reading should be within specifications as reported in the CAL CHECKTM Standard
Certificate. If the value is found out of the specifications, please check that the cuvettes are free
of fingerprints, oil or dirt and repeat validation. If results are still found out of specifications,
then recalibrate the instrument.
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CALIBRATION PROCEDURE
Note: It is possible to interrupt calibration procedure at any time by pressing CAL CHECK or
ON/OFF keys.
Warning:Do not calibrate the instrument with standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained.
When calibrating, only the selected range is affected.
•Turn the meter on by pressing ON/OFF.
•When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.
•Press and hold CAL CHECK for three
seconds to enter calibration mode . The
display will show “CAL” during
calibration procedure. The blinking
“ZERO” asks for instrument zeroing.
•Place the CAL CHECKTM Standard
HI 96750-11 Cuvette A into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
•Press ZERO/CFM and the lamp, cuvette
and detector icons will appear on the
display, depending on the measurement
phase.
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•After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready
for calibration. The blinking “READ” asks for
reading calibration standard.
•Remove the cuvette.
•Place the CAL CHECKTM Standard HI 96750-11
Cuvette B into the cuvette holder and ensure
that the notch on the cap is positioned securely
into the groove.
•Press READ/ /TIMER and the lamp, cuvette
and detector icons will appear on the display,
depending on the measurement phase.
•After measurement the instrument will show for
three seconds the CAL CHECKTM Standard value.
Note: If the display shows “STD HIGH”, the
standard value was too high. If the display
shows “STD LOW”, the standard value was
too low. Verify that both CAL CHECKTM
Standards HI 96750-11 Cuvettes, A and B are
free from fingerprints or dirt and that they are
inserted correctly.
Then the date of the last calibration (e.g.:
”01.08.2008”) appears on the display, or
“01.01.2008” if the factory calibration was
selected before. In both cases the year number
is blinking, ready for date input.
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•Press RANGE/GLP/ to edit the desired
month (01-12). If the key is kept pressed,
the month number is automatically
increased.
•Press RANGE/GLP/ to edit the desired
year (2000-2099). If the key is kept
pressed, the year number is automatically
increased.
•Press RANGE/GLP/ to edit the desired
day (01-31). If the key is kept pressed,
the day number is automatically increased.
Note: It is possible to change the editing
from day to year and to month by pressing
READ/ /TIMER.
•When the correct year has been set, press
ZERO/CFM or READ/ /TIMER to
confirm. Now the display will show the
month blinking.
•When the correct month has been set,
press ZERO/CFM or READ/ /TIMER
to confirm. Now the display will show
the day blinking.
•Press ZERO/CFM to save the calibration
date.
•The instrument displays “Stor” for one
second and the calibration is saved.
DATE INPUT
or
or
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It is possible to delete the calibration and
restore factory calibration.
•Press RANGE/GLP/ to enter GLP
mode
.
To display the calibration date:
•Press RANGE/GLP/ to enter GLP
mode
. The calibration month and day
will appear on the main display and the
year on the secondary display.
In the GLP mode, the last user calibration date can be consulted and the factory calibration can
be restored.
LAST CALIBRATION DATE
•If no calibration was performed, the
factory calibration message, “F.CAL” will
appear on the main display and the
instrument returns to measurement mode
after three seconds.
FACTORY CALIBRATION RESTORE
GLP
•The instrument will return automatically
to measurement mode by displaying
dashes on the LCD.
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