Hanna Instruments HI83325 User manual

INSTRUCTION MANUAL
HI83325
Multiparameter Photometer
for Nutrient Analysis

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use of this
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

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TABLE OF CONTENTS
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ............................................................................................... 5
2. SAFETY MEASURES............................................................................................................. 5
3. SPECIFICATIONS................................................................................................................. 6
4. ABBREVIATIONS ................................................................................................................ 7
5. DESCRIPTION..................................................................................................................... 8
5.1. GENERAL DESCRIPTION & INTENDED USE .................................................................. 8
5.2. PRECISION & ACCURACY............................................................................................ 8
5.3. FUNCTIONAL DESCRIPTION........................................................................................ 9
5.4. PRINCIPLE OF OPERATION....................................................................................... 10
5.5. OPTICAL SYSTEM..................................................................................................... 11
6. GENERAL OPERATIONS...................................................................................................... 12
6.1. POWER CONNECTION & BATTERY MANAGEMENT ....................................................... 12
6.2. MODE SELECTION ................................................................................................... 12
6.3. GENERAL SETUP ..................................................................................................... 13
6.4. CONTEXTUAL HELP.................................................................................................. 16
7. LOGGING DATA & DATA MANAGEMENT................................................................................ 17
7.1. LOGGING DATA ....................................................................................................... 17
7.2. ADDING SAMPLE & USER NAMES TO LOG DATA......................................................... 17
7.3. DATA MANAGEMENT................................................................................................ 18
8. NUTRIENT SAMPLES PREPARATION GUIDE.......................................................................... 19
8.1. INTRODUCTION TO PLANT NUTRIENTS...................................................................... 19
8.2. PREPARING NUTRIENT SAMPLES FOR ANALYSIS........................................................ 21
8.3. PROCEDURE FOR DILUTION FACTOR: 5 ..................................................................... 23
8.4. PROCEDURE FOR DILUTION FACTOR: 10 ................................................................... 24
8.5. PROCEDURE FOR DILUTION FACTOR: 50 ................................................................... 25
8.6. REMOVING TURBIDITY AND COLOR........................................................................... 26
9. PHOTOMETER MODE......................................................................................................... 27
9.1. METHOD SELECTION ............................................................................................... 27
9.2. COLLECTING & MEASURING SAMPLES AND REAGENTS................................................ 27
9.3. CUVETTE PREPARATION........................................................................................... 28
9.4. TIMERS & MEASUREMENT FUNCTIONS..................................................................... 30
9.5. CHEMICAL FORMULA & UNIT CONVERSION............................................................... 30

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TABLE OF CONTENTS
9.6. METER VALIDATION & CAL CHECK............................................................................. 31
9.7. ABSORBANCE MEASUREMENTS................................................................................ 32
10. PROBE MODE................................................................................................................. 33
10.1. pH MEASUREMENT............................................................................................... 33
10.2. pH CALIBRATION................................................................................................... 34
10.3. pH MESSAGES & WARNINGS ................................................................................. 35
10.4. pH GLP ................................................................................................................ 36
10.5. pH ELECTRODE CONDITIONING & MAINTENANCE..................................................... 37
11. METHOD PROCEDURES ................................................................................................... 39
11.1. AMMONIA LOW RANGE.......................................................................................... 39
11.2. AMMONIA MEDIUM RANGE ................................................................................... 42
11.3. AMMONIA HIGH RANGE ........................................................................................ 45
11.4. CALCIUM.............................................................................................................. 48
11.5. IRON(II)/(III) ....................................................................................................... 51
11.6. MAGNESIUM ........................................................................................................ 55
11.7. NITRATE............................................................................................................... 57
11.8. PHOSPHATE HIGH RANGE...................................................................................... 60
11.9. POTASSIUM ......................................................................................................... 63
11.10. SULFATE ............................................................................................................ 66
12. WARNINGS & ERRORS.................................................................................................... 68
13. STANDARD METHODS ..................................................................................................... 71
14. ACCESSORIES................................................................................................................. 72
14.1. REAGENT SETS...................................................................................................... 72
14.2. pH ELECTRODES ................................................................................................... 73
14.3. pH SOLUTIONS..................................................................................................... 74
14.4. OTHER ACCESSORIES ............................................................................................ 76
CERTIFICATION...................................................................................................................... 78
RECOMMENDATIONS FOR USERS............................................................................................ 78
WARRANTY ........................................................................................................................... 78

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PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further
assistance, please contact your local Hanna Instruments Office or email us at [email protected].
Each HI83325 is delivered in a rugged carrying case and is supplied with:
• Sample cuvette (4 pcs.)
• Sample cuvette cap (4 pcs.)
• Cloth for wiping cuvettes
• Scissors
• 100 mL plastic graduated beaker with cap
• 170 mL plastic graduated beaker
• 3 mL plastic pipette
• 5 mL graduated syringe
• 60 mL graduated syringe
• Graduated cylinder
•
• Spoon
• Funnel
• Filter paper
• Demineralizer bottle for 10 L of water
• Activated carbon for 50 tests
• USB cable
• 5 Vdc power adapter
• Instrument quality certificate
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in its original packing material with the supplied
accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required and follow
instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of
water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid
breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a
licensed waste disposal provider.
SAFETY MEASURES

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SPECIFICATIONS
3. SPECIFICATIONS
Measurement Channels 3 x optical channels
1 x digital electrode channel (pH measurement)
Photometer
Range 0.000 to 4.000 Abs
Resolution 0.001 Abs
Accuracy ±0.003 Abs @ 1.000 Abs
Light source Light Emitting Diode
Bandpass filter bandwidth 8 nm
Bandpass filter wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette types Round, 24.6 mm diameter
Number of methods 13
Probe
Range -2.00 to 16.00 pH (± 1000.0 mV)*
Resolution 0.01 pH (0.1 mV)
Accuracy ±0.01 pH (±0.2 mV) @ 25 °C / 77 °F
Temperature compensation ATC, -5.0 to 100.0 °C (23.0 to 212.0 °F)*
Calibration two-point, from five available buffers
(4.01, 6.86, 7.01, 9.18, 10.01 pH)
Electrode Intelligent pH / temperature electrode
Temperature
Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)
Resolution 0.1 °C (0.1 °F)
Accuracy ±0.5 °C @ 25 °C (±0.9 °F @ 77 °F)
Additional
Specifications
Logging 1000 readings (mixed photometer and electrode)
Display 128 x 64 pixel B/W LCD with backlight
USB-A (Host) functions Mass-storage host
USB-B (Device) functions Power input, mass-storage device
Battery life > 500 photometer measurements or 50 hours
of continuous pH measurement
Power supply
5 Vdc USB 2.0 power adapter / type micro-B
connector
3.7 Vdc Li-polymer rechargeable battery,
non-serviceable
Environment 0 to 50 °C (32 to 122 °F)
0 to 95% RH, non-serviceable
Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8")
Weight 1.0 kg (2.2 lbs.)
*Limits will be reduced to actual probe / sensor limits.

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ABBREVIATIONS
4. ABBREVIATIONS
Abs Absorbance
GLP Good Laboratory Practice
NIST National Institute of Standards and Technology
EPA US Environmental Protection Agency
g/L grams per liter (ppt)
µg/L micrograms per liter (ppb)
mg/L milligrams per liter (ppm)
mL milliliter
HR High Range
LR Low Range
MR Medium Range

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DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION & INTENDED USE
HI83325 multiparameter photometer is a compact and versatile meter with two measurement modes,
Photometer and Probe. Photometer mode includes a CAL Check™ feature and 13 different methods
that cover a wide variety of applications, making it ideal for both benchtop and portable operations.
With the CAL Check™ feature users are able to validate the performance of the instrument and
apply a user calibration (if necessary). Hanna Instruments CAL Check™ cuvettes are made with NIST
traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.
• Digital electrode input for pH measurements
• Certified CAL Check cuvettes to confirm meter functionality
• Dual purpose micro-USB flash drive
• Lithium polymer rechargeable battery
• Auto-off
• Absorbance mode
• User and sample name entry
• GLP features
5.2. PRECISION & ACCURACY
Precision is how closely repeated measurements are to one another. Precision is usually expressed as
standard deviation. Accuracy is defined as the closeness of a test result to the true value. Although
good precision suggests good accuracy, precise results can be inaccurate. The figure explains these
definitions. For each method, the accuracy is expressed in the related measurement section.

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DESCRIPTION
5.3. FUNCTIONAL DESCRIPTION
1. Splash-proof keypad
2. Liquid Crystal Display (LCD)
3. Indexing mark
4. Protective port covers
5. Light-blocking cover panel
6. Cuvette holder
7. ON/OFF power button
8. 3.5-mm TRRS (jack) input for digital electrodes
9. Standard USB host connector for data transfer to a USB flash drive
10. Micro-USB device connector for power or PC interface

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DESCRIPTION
Keypad Description
The keypad contains 12 direct keys and 3 functional keys with the following functions:
Press the functional key to perform the function displayed above it on the LCD.
Press to access the list of photometer methods.
Press to move up in a menu or a help screen, to increment a set value or to access second
level functions.
Press to toggle between photometer and probe (pH electrode) mode.
Press to move left in a menu or to decrement a set value.
Press to move down in a menu or a help screen, to decrement a set value or to access second
level functions.
Press to move right in a menu or to increment a set value.
Press to access the setup screen.
Press to log the current reading.
Press to review saved logs.
Press to exit the current screen.
Press to display the help screen.
ON/OFF power button
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices. Photometric chemical analysis is based on specific chemical reactions
between a sample and reagent to produce a light-absorbing compound.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law. If all other factors are constant, the concentration "c" can be calculated from the
absorbance of the substance.