Hanna Instruments HI83325 User manual

INSTRUCTION MANUAL
HI83325
Multiparameter Photometer
for Nutrient Analysis

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use of this
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

3
TABLE OF CONTENTS
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ............................................................................................... 5
2. SAFETY MEASURES............................................................................................................. 5
3. SPECIFICATIONS................................................................................................................. 6
4. ABBREVIATIONS ................................................................................................................ 7
5. DESCRIPTION..................................................................................................................... 8
5.1. GENERAL DESCRIPTION & INTENDED USE .................................................................. 8
5.2. PRECISION & ACCURACY............................................................................................ 8
5.3. FUNCTIONAL DESCRIPTION........................................................................................ 9
5.4. PRINCIPLE OF OPERATION....................................................................................... 10
5.5. OPTICAL SYSTEM..................................................................................................... 11
6. GENERAL OPERATIONS...................................................................................................... 12
6.1. POWER CONNECTION & BATTERY MANAGEMENT ....................................................... 12
6.2. MODE SELECTION ................................................................................................... 12
6.3. GENERAL SETUP ..................................................................................................... 13
6.4. CONTEXTUAL HELP.................................................................................................. 16
7. LOGGING DATA & DATA MANAGEMENT................................................................................ 17
7.1. LOGGING DATA ....................................................................................................... 17
7.2. ADDING SAMPLE & USER NAMES TO LOG DATA......................................................... 17
7.3. DATA MANAGEMENT................................................................................................ 18
8. NUTRIENT SAMPLES PREPARATION GUIDE.......................................................................... 19
8.1. INTRODUCTION TO PLANT NUTRIENTS...................................................................... 19
8.2. PREPARING NUTRIENT SAMPLES FOR ANALYSIS........................................................ 21
8.3. PROCEDURE FOR DILUTION FACTOR: 5 ..................................................................... 23
8.4. PROCEDURE FOR DILUTION FACTOR: 10 ................................................................... 24
8.5. PROCEDURE FOR DILUTION FACTOR: 50 ................................................................... 25
8.6. REMOVING TURBIDITY AND COLOR........................................................................... 26
9. PHOTOMETER MODE......................................................................................................... 27
9.1. METHOD SELECTION ............................................................................................... 27
9.2. COLLECTING & MEASURING SAMPLES AND REAGENTS................................................ 27
9.3. CUVETTE PREPARATION........................................................................................... 28
9.4. TIMERS & MEASUREMENT FUNCTIONS..................................................................... 30
9.5. CHEMICAL FORMULA & UNIT CONVERSION............................................................... 30

4
TABLE OF CONTENTS
9.6. METER VALIDATION & CAL CHECK............................................................................. 31
9.7. ABSORBANCE MEASUREMENTS................................................................................ 32
10. PROBE MODE................................................................................................................. 33
10.1. pH MEASUREMENT............................................................................................... 33
10.2. pH CALIBRATION................................................................................................... 34
10.3. pH MESSAGES & WARNINGS ................................................................................. 35
10.4. pH GLP ................................................................................................................ 36
10.5. pH ELECTRODE CONDITIONING & MAINTENANCE..................................................... 37
11. METHOD PROCEDURES ................................................................................................... 39
11.1. AMMONIA LOW RANGE.......................................................................................... 39
11.2. AMMONIA MEDIUM RANGE ................................................................................... 42
11.3. AMMONIA HIGH RANGE ........................................................................................ 45
11.4. CALCIUM.............................................................................................................. 48
11.5. IRON(II)/(III) ....................................................................................................... 51
11.6. MAGNESIUM ........................................................................................................ 55
11.7. NITRATE............................................................................................................... 57
11.8. PHOSPHATE HIGH RANGE...................................................................................... 60
11.9. POTASSIUM ......................................................................................................... 63
11.10. SULFATE ............................................................................................................ 66
12. WARNINGS & ERRORS.................................................................................................... 68
13. STANDARD METHODS ..................................................................................................... 71
14. ACCESSORIES................................................................................................................. 72
14.1. REAGENT SETS...................................................................................................... 72
14.2. pH ELECTRODES ................................................................................................... 73
14.3. pH SOLUTIONS..................................................................................................... 74
14.4. OTHER ACCESSORIES ............................................................................................ 76
CERTIFICATION...................................................................................................................... 78
RECOMMENDATIONS FOR USERS............................................................................................ 78
WARRANTY ........................................................................................................................... 78

5
PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully. For further
assistance, please contact your local Hanna Instruments Office or email us at [email protected].
Each HI83325 is delivered in a rugged carrying case and is supplied with:
• Sample cuvette (4 pcs.)
• Sample cuvette cap (4 pcs.)
• Cloth for wiping cuvettes
• Scissors
• 100 mL plastic graduated beaker with cap
• 170 mL plastic graduated beaker
• 3 mL plastic pipette
• 5 mL graduated syringe
• 60 mL graduated syringe
• Graduated cylinder
•
• Spoon
• Funnel
• Filter paper
• Demineralizer bottle for 10 L of water
• Activated carbon for 50 tests
• USB cable
• 5 Vdc power adapter
• Instrument quality certificate
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
damaged or defective item must be returned in its original packing material with the supplied
accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required and follow
instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of
water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid
breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a
licensed waste disposal provider.
SAFETY MEASURES

6
SPECIFICATIONS
3. SPECIFICATIONS
Measurement Channels 3 x optical channels
1 x digital electrode channel (pH measurement)
Photometer
Range 0.000 to 4.000 Abs
Resolution 0.001 Abs
Accuracy ±0.003 Abs @ 1.000 Abs
Light source Light Emitting Diode
Bandpass filter bandwidth 8 nm
Bandpass filter wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette types Round, 24.6 mm diameter
Number of methods 13
Probe
Range -2.00 to 16.00 pH (± 1000.0 mV)*
Resolution 0.01 pH (0.1 mV)
Accuracy ±0.01 pH (±0.2 mV) @ 25 °C / 77 °F
Temperature compensation ATC, -5.0 to 100.0 °C (23.0 to 212.0 °F)*
Calibration two-point, from five available buffers
(4.01, 6.86, 7.01, 9.18, 10.01 pH)
Electrode Intelligent pH / temperature electrode
Temperature
Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)
Resolution 0.1 °C (0.1 °F)
Accuracy ±0.5 °C @ 25 °C (±0.9 °F @ 77 °F)
Additional
Specifications
Logging 1000 readings (mixed photometer and electrode)
Display 128 x 64 pixel B/W LCD with backlight
USB-A (Host) functions Mass-storage host
USB-B (Device) functions Power input, mass-storage device
Battery life > 500 photometer measurements or 50 hours
of continuous pH measurement
Power supply
5 Vdc USB 2.0 power adapter / type micro-B
connector
3.7 Vdc Li-polymer rechargeable battery,
non-serviceable
Environment 0 to 50 °C (32 to 122 °F)
0 to 95% RH, non-serviceable
Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8")
Weight 1.0 kg (2.2 lbs.)
*Limits will be reduced to actual probe / sensor limits.

7
ABBREVIATIONS
4. ABBREVIATIONS
Abs Absorbance
GLP Good Laboratory Practice
NIST National Institute of Standards and Technology
EPA US Environmental Protection Agency
g/L grams per liter (ppt)
µg/L micrograms per liter (ppb)
mg/L milligrams per liter (ppm)
mL milliliter
HR High Range
LR Low Range
MR Medium Range

8
DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION & INTENDED USE
HI83325 multiparameter photometer is a compact and versatile meter with two measurement modes,
Photometer and Probe. Photometer mode includes a CAL Check™ feature and 13 different methods
that cover a wide variety of applications, making it ideal for both benchtop and portable operations.
With the CAL Check™ feature users are able to validate the performance of the instrument and
apply a user calibration (if necessary). Hanna Instruments CAL Check™ cuvettes are made with NIST
traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.
• Digital electrode input for pH measurements
• Certified CAL Check cuvettes to confirm meter functionality
• Dual purpose micro-USB flash drive
• Lithium polymer rechargeable battery
• Auto-off
• Absorbance mode
• User and sample name entry
• GLP features
5.2. PRECISION & ACCURACY
Precision is how closely repeated measurements are to one another. Precision is usually expressed as
standard deviation. Accuracy is defined as the closeness of a test result to the true value. Although
good precision suggests good accuracy, precise results can be inaccurate. The figure explains these
definitions. For each method, the accuracy is expressed in the related measurement section.

9
DESCRIPTION
5.3. FUNCTIONAL DESCRIPTION
1. Splash-proof keypad
2. Liquid Crystal Display (LCD)
3. Indexing mark
4. Protective port covers
5. Light-blocking cover panel
6. Cuvette holder
7. ON/OFF power button
8. 3.5-mm TRRS (jack) input for digital electrodes
9. Standard USB host connector for data transfer to a USB flash drive
10. Micro-USB device connector for power or PC interface

10
DESCRIPTION
Keypad Description
The keypad contains 12 direct keys and 3 functional keys with the following functions:
Press the functional key to perform the function displayed above it on the LCD.
Press to access the list of photometer methods.
Press to move up in a menu or a help screen, to increment a set value or to access second
level functions.
Press to toggle between photometer and probe (pH electrode) mode.
Press to move left in a menu or to decrement a set value.
Press to move down in a menu or a help screen, to decrement a set value or to access second
level functions.
Press to move right in a menu or to increment a set value.
Press to access the setup screen.
Press to log the current reading.
Press to review saved logs.
Press to exit the current screen.
Press to display the help screen.
ON/OFF power button
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices. Photometric chemical analysis is based on specific chemical reactions
between a sample and reagent to produce a light-absorbing compound.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law. If all other factors are constant, the concentration "c" can be calculated from the
absorbance of the substance.

11
DESCRIPTION
Lambert Beer Law:
-log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I= intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
5.5. OPTICAL SYSTEM
Instrument Block Diagram
The internal reference system (reference detector) of the HI83325 photometer compensates for any
drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for
your blank (zero) measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher
luminous efficiency, providing more light while using less power. They also produce little heat, which
could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas
tungsten lamps have poor blue / violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be
received. The end result is higher measurement stability and less wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette
imperfections and scratches, eliminating the need to index the cuvette.

12
GENERAL OPERATIONS
6. GENERAL OPERATIONS
6.1. POWER CONNECTION & BATTERY MANAGEMENT
The meter can be powered from an AC / DC adapter (included) or from the built-in rechargeable
battery.
The meter will perform an auto-diagnostic test when it is first powered on. During this test, the Hanna
Instruments logo will appear on the LCD. After 5 seconds, if the test was successful, the last method
used will appear on the display.
The battery icon on the LCD will indicate the battery status:
• battery is charging from external adapter • battery fully charged (meter connected to AC / DC adapter)
• battery capacity (no external adapter) • battery near 0% (no external adapter)
• battery exhausted (no external adapter)
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes
after a Zero measurement). If a photometer measurement is on the screen, an auto-log is created
before shutdown.
6.2. MODE SELECTION
The HI83325 has two operational modes: Photometer and Probe.
Photometer mode enables on-demand measurement of a cuvette using the integrated optical system.
Probe mode enables continuous measurement using a Hanna digital electrode connected to the
3.5 mm port.
To switch between Photometer mode and Probe mode, use the MODE key.
Note: The active mode cannot be switched while in Setup, Recall or Method menus.

13
GENERAL OPERATIONS
6.3. GENERAL SETUP
Press the SETUP key to enter in Setup menu, highlight desired option using the  keys and
press Select.
CAL Check (Photometer Mode Only)
Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are
displayed on the screen. To start a new CAL Check press Check and follow the prompts on the screen.
Temperature Unit (pH Only)
Option: °C or °F
Press the functional key to select the desired temperature unit.
Backlight
Values: 0 to 8
Press Modify to access the backlight intensity. Use the functional keys or the  keys to increase or
decrease the value. Press Accept to confirm or press the ESC key to return to the Setup menu without
saving the new value.
Contrast
Values: 0 to 20
Press Modify to change the display’s contrast. Use the functional keys or the  keys to increase
or decrease the value. Press Accept to confirm the value or the ESC key to return to the Setup menu
without saving the new value.

14
GENERAL OPERATIONS
Date & Time
Press Modify to change the date and time. Press the functional keys or the  keys to highlight the
value to be modified (year, month, day, hour, minute or second). Use the  keys to change the
value. Press Accept to confirm or ESC key to return to the Setup without saving the new date or time.
Time Format
Option: AM/PM or 24-hour
Press the functional key to select the desired time format.
Date Format
Option: DD/MM/YYYY, MM/DD/YYYY, YYYY/MM/DD, YYYY-MM-DD, Mon DD, YYYY,
DD-Mon-YYYY, YYYY-Mon-DD
Press Modify to change the date format. Use the  keys to select the desired format. Press Select
to confirm or the ESC key to return to the Setup menu without saving the new format.
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal separator. The decimal separator is used on the
measurement screen and CSV (Comma-Separated Values) files.

15
GENERAL OPERATIONS
Language
Option: Português, Deutsch, English, Español, Français, Italiano, Dutch
Press Modify to change the language. Use the  keys to select the desired language. Press Select
to change the language.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when
the pressed key is not active or an error is detected. Press the functional key to enable or disable the
beeper.
Instrument ID
Option: 0 to 999999
This option is used to set the instrument’s ID (identification number). Press Modify to access the
instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified.
Press the  keys in order to set the desired value. Press Accept to confirm the value or press the
ESC key to return to the Setup menu without saving the new value.
Meter Information
Press Select to view the model, serial number, firmware version and selected language. Press the ESC
key to return to the Setup menu.

16
GENERAL OPERATIONS
Probe Information (pH Mode Only)
Press Select to view model number, serial number and firmware version for the connected probe. Press
the ESC key to return to the Setup menu.
6.4. CONTEXTUAL HELP
HI83325 offers an interactive contextual help mode that assists the user at any time.
To access the help screen press the HELP key. The instrument will display additional information
related to the current screen. To read all the available information, scroll the text using the  keys.
Press the ESC key to return to the previous screen.

17
LOGGING DATA & DATA MANAGEMENT
7. LOGGING DATA & DATA MANAGEMENT
The instrument features a data log function to help you keep track of all your analysis. The data log
can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the
LOG and RECALL keys.
7.1. LOGGING DATA
Press the LOG key and the last valid measurement will be stored with a date and time stamp. Only
valid measurements can be stored.
7.2. ADDING SAMPLE & USER NAMES TO LOG DATA
A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample
ID or User ID then press Modify. Sample ID and user ID are entered using the alphanumeric multi-
tapping keypad.
Enter one character at a time by pressing the key with the assigned character repeatedly until the
desired character is highlighted. For reference, a list of the characters available for the current key will
be shown under the text box.
The character will be entered after a two-second delay or after another key is pressed.
Press Accept to update the sample or user ID.
Press functional key to delete the last character.
Press Clear to delete all of the characters.
Press the ESC key to discard all changes and return to the previous screen.

18
LOGGING DATA & DATA MANAGEMENT
7.3. DATA MANAGEMENT
Viewing & Deleting
Data can be viewed, deleted and exported to a USB drive or a PC by pressing the RECALL key. Use
the  keys to scroll through the saved logs. Press Info to view additional information about the
selected log.
Use Delete to erase logged data. After pressing Delete the prompt on the display will confirm the action.
Press No or the ESC key to return to the previous screen.
Press Yes to delete the selected log.
Press Del All to erase all the logged data. If Del All is pressed the prompt on the display will confirm
the action. Press Yes to delete all logged data, No or the ESC key to return to the log recall.
Data Export
Log data can be exported to a USB flash drive or to a PC. To access data export functions, press the
RECALL key then Export.
Use the  keys to select the desired export location.
For export to flash drive, insert the USB flash drive into the dedicated port at the back of the meter
labeled HOST USB, then follow the on-screen prompts.
For export to PC, connect the meter to a PC using the supplied micro-USB cable. Insert the cable into
the port at the back of the meter labeled PC PWR. Follow the on-screen prompts. When the meter
says PC connected, the meter will appear as a removable disk. Use a file manager (such as Windows
Explorer or Mac Finder) to move the file from the meter to the PC.
Log data is exported as a single file (HI83325.csv) containing all logged photometer and probe data.
The CSV file may be opened with a text editor or spreadsheet application.

19
NUTRIENT SAMPLES PREPARATION GUIDE
8. NUTRIENT SAMPLES PREPARATION GUIDE
8.1. INTRODUCTION TO PLANT NUTRIENTS
The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and
potassium (K). They are called the macronutrients while other elements, needed by plants in smaller
amounts, are called microelements. In hydroponics, plants need a balanced nutritive solution,
composed of macro and microelements.
Shortage or excess of even only one nutritive element may cause an imbalance in plant physiology
and in the absorption of the other nutrients. Nutrients shortages may result in irregular plant growth,
low resistance to diseases, scarce production both in quantity and quality, while nutrients excess may
cause waste of fertilizer, pollution of the groundwater and the possible accumulation of dangerous
substances in the crops produced.
Nitrogen
Nitrogen (N) is mostly absorbed by plants as nitrates (NO3
-) and, in smaller amount, in the form of
ammonium (NH4
+). In hydroponics, an adequate ratio between the two forms is generally used in
nutritive solutions.
PRESENT IN proteins, enzymes, chlorophyll, hormones, vitamins, DNA and RNA
ACTION
• is fundamental for plants in phase of growth
• promotes lengthening of trunks and sprouts
• increases the production of foliage
• helps to absorb other nutrients (in particular phosphorus)
• assists a bigger production for both size and number of fruits
SHORTAGE EFFECTS
• slower growth
• smaller leaves
• yellowing of leaves
• smaller fruits
• premature ripening
EXCESS EFFECT
• reduction in resistance to diseases and atmospheric agents
• increase of water demand (caused by an excessive production of leaves)
• bad quality of fruits
• delayed ripening
• reduction in potassium absorption

20
NUTRIENT SAMPLES PREPARATION GUIDE
Phosphorus
Phosphorus (P) has an important role in many fundamental biochemical and physiological processes.
Plants take up phosphorus in the form of phosphate ion (PO4
3-).
PRESENT IN DNA and RNA, ATP, ADP
ACTION
• stimulates the roots growth
• stimulates blooming
• stimulates fecundation and ripeness
• strengthens the plant tissues
• is necessary in the formation of seeds
SHORTAGE EFFECTS
• delayed ripening
• slower growth
• small leaves
• decrease of production (smaller fruits and difficult seeds formation)
• reduction of root system
EXCESS EFFECT
• premature ripening
• excess of fruit-setting
• negative effects on the absorption of some microelements such as iron,
zinc, boron and copper
Potassium
Potassium (K) is essential in proteic synthesis. The problem of lack of potassium is quite frequent in
calcareous soils.
Potassium is absorbed as K+.
PRESENT IN tissues responsible for the growth of plants (primary and secondary
meristems), embryos and cell vacuole
ACTION
• improves the quality of fruits and flowers
• gives more resistance both to frost and to diseases caused by fungi
(increases the cuticular thickness)
• regulates the cellular turgidity (helps to regulate the osmotic processes
and increases the resistance to dryness)
• regulates the stomatic opening and closing (it means a strong influence
on transpiration and photosynthesis)
SHORTAGE EFFECTS
• slower growth
• smaller fruits, less colored and less preserved
• increase of transpiration
• less resistance to the cold
EXCESS EFFECT
• reduced absorption of calcium and magnesium
• increase of water consumption
• increase of the substrate salinity