Hanna Instruments HI96802 User manual

HI96800
Refractometer for Refractive
Index and Brix Measurements
INSTRUCTION MANUAL

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using
this instrument.
This manual will provide you with the necessary informa‑
tion for correct use of this instrument, as well as a precise
idea of its versatility.
If you need additional technical information, do not
hesitate to e‑mail us at [email protected] or view our
worldwide contact list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895, USA.

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TABLE OF CONTENTS
Preliminary Examination ........................................................
General Description................................................................
Specifications........................................................................
Principle of Operation............................................................
Functional Description............................................................
Display Elements...................................................................
Measurement Guidelines ........................................................
Calibration Procedure.............................................................
Measurement Procedure.........................................................
To Change Measurement Unit.................................................
To Change Temperature Unit ..................................................
Making a Standard % Brix Solution .......................................
Battery Replacement..............................................................
Error Messages .....................................................................
Accessories............................................................................
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4
5
7
9
11
15
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8
12
10
14
16
17
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Remove the instrument from the packing materials and examine carefully
to ensure no damage has occurred during shipping. If any damage has
occurred, contact your local Hanna Instruments Office.
Each HI96800 instrument is supplied with:
• 9 V battery
• Instruction manual
• Plastic pipette
• Quality Certificate
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
PRELIMINARY EXAMINATION

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The HI96800 Digital Refractometer is a rugged portable, water resistant device
that benefits from Hanna Instruments’ years of experience as a manufacturer
of analytical instruments.
The HI96800 is an optical instrument that is both simple and quick. Samples
are measured after a simple user calibration with deionized or distilled water.
Within seconds the instrument measures the refractive index of the sample.
Reading can also be displayed with sucrose temperature compensation
(nD20) or % Brix.
TheHI96800digitalrefractometereliminatestheuncertainityassociatedwith
mechanicalrefractometersandiseasilyportableformeasurementsinthefield.
The measurement technique and temperature compensation employ metho‑
dologyrecommendedintheICUMSA MethodsBook(InternationalCommision
for Uniform Methods of Sugar Analysis).
Temperature (in °C or °F) is displayed simultaneously with the measurement
on the large dual level display along with icons for Low Power and other
helpful message codes.
Key features include:
• Dual‑level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non‑use.
GENERAL DESCRIPTION

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SPECIFICATIONS
Range
nD: 1.3300 to 1.5080
nD20:1.3330 to 1.5040
% Brix: 0.0 to 85.0
Temperature: 0.0 to 80.0 °C; 32.0 to 176.0 °F
Resolution
nD: 0.0001
nD20:0.0001
% Brix: 0.1
Temperature: 0.1 °C; 0.1 °C
Accuracy
nD: ±0.0005
nD20:±0.0005
% Brix: ±0.2
Temperature: ±0.3 °C; ±0.5 °F
Temperature
Compensation
Automatic between 10.0 and 40.0 °C
(50.0 ‑ 104.0 °F)
Measurement Time Approximately 1.5 seconds
Minimum Sample
Volume 100 µL (cover prism totally)
Light Source Yellow LED
Sample Cell Stainless Steel ring and flint glass prism
Case Material ABS
Enclosure Rating IP65
Battery Type / Life 9V / 5000 readings
Auto‑Off After 3 minutes of non‑use
Dimensions 192 x 102 x 69mm (7.6 x 4.1 x 2.7”)
Mass 350 g (12.3 oz.)

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Refractive Index is an optical characteristic of a substance and the number of
dissolved particles in it. Refractive Index is defined as the ratio of the speed
of light in empty space to the speed of light in the substance. A result of this
property is that light will “bend”, or change direction, when it travels through
a substance of different refractive index. This is called refraction.
When passing from a material with a higher to lower refractive index, there
is a critical angle at which an incoming beam of light can no longer refract,
but will instead be reflected off the interface. The critical angle can be used
to easily calculate the refractive index according to the equation:
sin (θcritical) = n2/ n1
Wheren2istherefractiveindexofthelower‑densitymedium;n1istherefractive
index of the higher‑density medium.
In the HI96800, light from an LED passes through a prism in contact with
the sample. An image sensor determines the critical angle at which the light
is no longer refracted through the sample.
PRINCIPLE OF OPERATION

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9. Battery Cover
10. Battery Compartment
1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. RANGE Key (User Measurement Unit)
7. ON/OFF
8. Stainless Steel Sample Well and Prism
TOP VIEW
BOTTOM
FUNCTIONAL DESCRIPTION

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1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Units
7. Automatic Temperature Compensation
(blinks when temperature exceeds 10‑40 °C / 50‑104 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when blinking,
temperature has exceeded operation range: 0‑80 °C / 32‑176 °F)
DISPLAY ELEMENTS

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• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well”
located over the prism.
• Do not expose instrument or prism to solvents that will damage it. This
includes most organic solvents and extremely hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample
with a soft tissue and rinse sample well with deionized or distilled water
between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
• To reduce the effects of evaporation or absorption of water when taking
readings over a period of time, the prism and sample well can be covered
with plastic wrap.
MEASUREMENT GUIDELINES