Hanna Instruments HI97728 User manual

INSTRUCTION MANUAL
HI97728
Nitrate
Photometer

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the
instrument.
This manual will provide you with the necessary information
for correct use of the instrument, as well as a precise idea of its
versatility.
If you need additional technical information, do not hesitate to
e‑mail us at [email protected] or view our worldwide contact
list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

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TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION ..........................................................................4
2. SAFETY MEASURES........................................................................................4
3. SPECIFICATIONS............................................................................................5
4. ABBREVIATIONS ...........................................................................................5
5. DESCRIPTION................................................................................................6
5.1. GENERAL DESCRIPTION AND INTENDED USE ...........................................6
5.2. FUNCTIONAL DESCRIPTION ....................................................................7
5.3. PRECISION AND ACCURACY ....................................................................8
5.4. PRINCIPLE OF OPERATION .....................................................................8
5.5. OPTICAL SYSTEM...................................................................................9
6. GENERAL OPERATIONS...................................................................................9
6.1. METER VALIDATION: CAL CHECK/CALIBRATION......................................... 9
6.2. CHEMICAL FORMULA/UNIT CONVERSION ..............................................11
6.3. GLP....................................................................................................11
6.4. LOGGING DATA/LOG RECALL ................................................................. 12
6.5. GENERAL SETUP.................................................................................. 12
6.6. REAGENTS/ACCESSORIES..................................................................... 15
6.7. CONTEXTUAL HELP ..............................................................................15
6.8. BATTERY MANAGEMENT.......................................................................16
7. PHOTOMETER..............................................................................................16
7.1. PROPER USE OF POWDER PACKET ........................................................ 16
7.2. CUVETTE PREPARATION ....................................................................... 17
8. METHOD PROCEDURE ..................................................................................19
9. ERROR DESCRIPTIONS .................................................................................22
10. BATTERY REPLACEMENT ............................................................................. 23
11. ACCESSORIES............................................................................................ 24
11.1. REAGENT SETS..................................................................................24
11.2. OTHER ACCESSORIES......................................................................... 24
CERTIFICATION.................................................................................................25
RECOMMENDATIONS FOR USERS.......................................................................26
WARRANTY ......................................................................................................27
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PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully to
make sure that no damage has occurred during shipping. Notify your nearest Hanna
Customer Service Center if damage is observed.
Each HI97728C is delivered in a rugged carrying case and is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• A ZERO - CAL CheckCuvette A
• HI97728B -CAL Check Cuvette B for Nitrate
• Cloth for wiping cuvettes
• Scissors
• 1.5V AA Alkaline batteries
• Instruction manual
• Meter quality certificate
• CAL Check standard certificate
Each HI97728 is delivered in a cardboard box and is supplied with:
• Sample cuvette (2 pcs.)
• Sample cuvette cap (2 pcs.)
• Plastic stopper (2 pcs.)
• 1.5V AA Alkaline batteries
• Instruction manual
• Meter quality certificate
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with
the supplied accessories.
2. SAFETY MEASURES
•The chemicals contained in the reagent kits may be hazardous if
improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and
follow instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty
of water. If reagent contacts skin, rinse the affected area thoroughly with water.
Avoid breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a
licensed waste disposal provider.
SAFETY MEASURES

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SPECIFICATIONS
3. SPECIFICATIONS
Nitrate
Range 0.0 to 30.0 mg/L (as NO3-N)
Resolution 0.1 mg/L
Accuracy ±0.5 mg/L ±10% of reading at 25 °C
Method Adaptation of Cadmium Reduction method
Measurement
System
Light source Light Emitting Diode
Bandpass filter 525 nm
Bandpass filter
bandwidth 8 nm
Bandpass filter
wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside)
Additional
Specifications
Auto logging 50 readings
Display 128 x 64 pixel B/W LCD with backlight
Auto-off After 15 minutes of inactivity
(30 minutes before a READ measurement)
Battery type 1.5 V AA Alkaline (3 pcs.)
Battery life > 800 measurements (without backlight)
Environment 0 to 50 °C (32 to 122 °F);
0 to 100% RH, non-serviceable
Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")
Weight (with batteries) 380 g (13.4 oz.)
Case ingress
protection rating IP67, floating case
4. ABBREVIATIONS
mg/L milligrams per liter (ppm)
mL milliliter
°C degree Celsius
°F degree Fahrenheit
LED Light Emitting Diode
EPA US Environmental Protection Agency
HDPE High Density Polyethylene
GLP Good Laboratory Practice
NIST National Institute of Standards and Technology
ABBREVIATIONS

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DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION AND INTENDED USE
The HI97728 is an auto-diagnostic portable meter that benefits from Hanna’s years
of experience as a manufacturer of analytical instruments. It has an advanced optical
system that uses a Light Emitting Diode (LED) and a narrow band interference filter that
allows for accurate and repeatable readings.
The optical system is sealed from outside dust, dirt and water. The meter uses an
exclusive positive-locking system to ensure that the cuvettes are placed into the holder
in the same position every time.
With the CAL Check functionality, users are able to validate the performance of the
instrument at any time and apply a user calibration (if necessary). Hanna’s CAL Check
cuvettes are made with NIST traceable standards.
The built-in tutorial mode guides the users step-by-step through the measurement
process. It includes all steps required for sample preparation, the required reagents
and quantities.
The HI97728 meter measures the nitrate content in water samples in the 0.0 to 30.0
mg/L (ppm) as NO3-N range. The method is an adaptation of the Cadmium Reduction
method.
The HI97728 photometer is a compact and versatile meter suitable for field or bench
measurements, featuring a:
• Sophisticated optical system
• Meter validation using certified CAL Check cuvettes
• Tutorial mode guides the user step-by-step
• Auto logging
• Waterproof IP67, floating case
• GLP features

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DESCRIPTION
5.2. FUNCTIONAL DESCRIPTION
1) ON/OFF power button 3) Liquid Crystal Display (LCD) 5) Indexing mark
2) Keypad 4) Cuvette holder 6) Battery cover
Keypad Description
The keypad contains 3 direct keys and 3 functional keys with the following functions:
Press the functional keys to perform the function displayed above them on the LCD.
Press and hold to power off/on. Press briefly to return to the previous screen.
Press to access the menu screen.
Press to display the context-sensitive help menu.

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DESCRIPTION
5.3. PRECISION AND ACCURACY
Precision is how closely repeated
measurements are to one
another. Precision is usually
expressed as standard deviation
(SD).
Accuracy is defined as the
closeness of a test result to the
true value.
Although good precision suggests
good accuracy, precise results can
be inaccurate. The figure explains
these definitions.
For each method, the accuracy is expressed in the related measurement section.
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic
radiation and matter. When a light beam crosses a substance, some of the radiation
may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical
path length through the matter and on the physical-chemical characteristics of the
substance according to the Lambert-Beer Law:
-log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I= intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration “c” can be calculated from the absorbance of the substance
as the other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a
sample and reagent to produce a light-absorbing compound.

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DESCRIPTION GENERAL OPERATIONS
5.5. OPTICAL SYSTEM
Instrument Block Diagram
Theinternalreference system (reference detector) oftheHI97728 photometer compensates
for any drifts due to power fluctuations or ambient temperature changes, providing a
stable source of light for your blank (zero) measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a
much higher luminous efficiency, providing more light while using less power. They also
produce little heat, which could otherwise affect electronic stability. LEDs are available in
a wide array of wavelengths, whereas tungsten lamps have poor blue/violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter,
stronger signal to be received. The end result is higher measurement stability and less
wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from
cuvette imperfections and scratches, eliminating the need to index the cuvette.
6. GENERAL OPERATIONS
6.1. METER VALIDATION: CAL CHECK/CALIBRATION
Validation of the HI97728 involves verifying the concentration of the certified CAL
Check standards. The CAL Check screen guides the user step-by-step through the
validation process and user calibration (if necessary).
WARNING: Do not use any solutions/standards other than the Hanna®CAL Check
Standards. For accurate validation and calibration results, please perform these at
room temperature (18 to 25 °C; 64.5 to 77.0 °F).
Note: CAL Check Standards will not read the specified value in measurement mode.
Protect the CAL Check cuvettes from direct sunlight by keeping them in the original
packing. Store between +5 °C and +30 °C (41 - 86 °F), do not freeze.

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GENERAL OPERATIONS
To perform a CAL Check:
1. Press CAL Check key from measurement mode.
The “Not Available“ message or the date/time
and status of the last CAL Check will be displayed
on the screen.
2. Press the Check key to start a new CAL Check. Press
the key at any time to abort the validation
process.
3. Use the keys to enter the certificate value
of the calibration standard found on the CAL Check
Standard Certificate. Press Next to continue.
Note: This value will be saved in the instrument for future validation. If a new set
of calibration standards is obtained please update the certificate value.
4. Insert the A ZERO CAL Check Cuvette A then press
Next to continue. The “Please Wait...“ message
will be displayed during the measurement.
5. Insert the HI97728B CAL Check Cuvette B then
press Next to continue. The “Please Wait...“
message will be displayed during the measurement.
6. When the CAL Check is complete the display will
show one of the following messages and the value
obtained during the measurement:
- “PASSED“: The measured value is within the accuracy
specification, no user calibration is required.