Hanna Instruments HI97700 User manual

INSTRUCTION MANUAL
HI97700
Ammonia Low
Range
Photometer

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the
instrument.
This manual will provide you with the necessary information for correct
use of the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to
e‑mail us at [email protected] or view our worldwide contact
list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Dear
Customer,

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TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION........................................................................
2. SAFETY MEASURES......................................................................................
3. SPECIFICATIONS..........................................................................................
4. ABBREVIATIONS..........................................................................................
5. DESCRIPTION..............................................................................................
5.1. GENERAL DESCRIPTION AND INTENDED USE...........................................
5.2. FUNCTIONAL DESCRIPTION....................................................................
5.3. PRECISION AND ACCURACY....................................................................
5.4. PRINCIPLE OF OPERATION.....................................................................
5.5. OPTICAL SYSTEM ..................................................................................
6. GENERAL OPERATIONS.................................................................................
6.1. METER VALIDATION: CAL CHECK / CALIBRATION ......................................
6.2. CHEMICAL FORMULA / UNIT CONVERSION..............................................
6.3. GLP .....................................................................................................
6.4. LOGGING DATA/LOG RECALL...................................................................
6.5. GENERAL SETUP...................................................................................
6.6. REAGENTS/ACCESSORIES ......................................................................
6.7. CONTEXTUAL HELP................................................................................
6.8. BATTERY MANAGEMENT........................................................................
7. PHOTOMETER..............................................................................................
7.1. PROPER USE OF DROPPER BOTTLE.........................................................
7.2. CUVETTE PREPARATION.........................................................................
8. METHOD PROCEDURE ..................................................................................
9. ERROR DESCRIPTIONS .................................................................................
10. BATTERY REPLACEMENT.............................................................................
11. ACCESSORIES............................................................................................
11.1. REAGENT SETS ...................................................................................
11.2. OTHER ACCESSORIES..........................................................................
CERTIFICATION................................................................................................
RECOMMENDATIONS FOR USERS......................................................................
WARRANTY .....................................................................................................
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1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully to
make sure that no damage has occurred during shipping. Notify your nearest Hanna
Customer Service Center if damage is observed.
Each HI97700C is supplied with:
• Sample cuvette (2 pcs.)
• Sample cap (2 pcs.)
• Plastic stopper (2 pcs.)
• A ZERO ‑ CAL Check Cuvette A
• HI97700B ‑ CAL Check Cuvette B for Ammonia LR
• Cloth for wiping cuvettes
• Scissors
• Alkaline batteries: 1.5V AA (3 pcs.)
• Instruction manual
• Meter quality certificate
• CAL Check standard certificate
Each HI97700 is supplied with:
• Sample cuvette (2 pcs.)
• Sample cap (2 pcs.)
• Plastic stopper (2 pcs.)
• Alkaline batteries: 1.5V AA (3 pcs.)
• Instruction manual
• Meter quality certificate
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with the
supplied accessories.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if
improperly handled.
• Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and
follow instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty
of water. If reagent contacts skin, rinse the affected area thoroughly with water.
Avoid breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact
a licensed waste disposal provider.
PRELIMINARY EXAMINATIONSAFETY MEASURES

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Ammonia
Low Range
Range 0.00 to 3.00 mg/L (as NH3‑N)
Resolution 0.01 mg/L
Accuracy ±0.04 mg/L ±4% of reading at 25 °C
Method
Adaptation of the ASTM Manual of Water
and Environmental Technology,
D1426 Nessler method
Measurement
System
Light source Light Emitting Diode
Bandpass filter 420 nm
Bandpass filter
bandwidth 8 nm
Bandpass filter
wavelength accuracy ±1.0 nm
Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside)
Additional
Specifications
Auto logging 50 readings
Display 128 x 64 pixel B/W LCD with backlight
Auto‑off After 15 minutes of inactivity
(30 minutes before a READ measurement)
Battery type Alkaline 1.5 V AA (3 pcs.)
Battery life > 800 measurements (without backlight)
Environment 0 to 50 °C (32 to 122 °F);
0 to 100% RH, non‑serviceable
Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")
Weight (with batteries) 380 g (13.4 oz.)
Case ingress protection
rating IP 67, floating case
3. SPECIFICATIONS
SPECIFICATIONS
4. ABBREVIATIONS
mg/L milligrams per liter (ppm)
mL milliliter
°C degree Celsius
°F degree Fahrenheit
LED Light Emitting Diode
HDPE High density polyethylene
GLP Good Laboratory Practice
ABBREVIATIONS

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DESCRIPTION
5. DESCRIPTION
5.1. GENERAL DESCRIPTION AND INTENDED USE
The HI97700 is an auto‑diagnostic portable meter that benefits from Hanna’s years
of experience as a manufacturer of analytical instruments. It has an advanced optical
system that uses a Light Emitting Diode (LED) and a narrow band interference filter
that allows for accurate and repeatable readings.
The optical system is sealed from outside dust, dirt and water. The meter uses an
exclusive positive‑locking system to ensure that the cuvettes are placed into the holder
in the same position every time.
With the CAL Check functionality, users are able to validate the performance of the
instrument at any time and apply a user calibration (if necessary). Hanna's CAL Check
cuvettes are made with NIST traceable standards.
The built in Tutorial mode guides users step‑by‑step through the measurement process. It
includes all steps required for sample preparation, the required reagents and quantities.
The HI97700 meter measures ammonia in water samples from 0.00 to 3.00 mg/L
(ppm). The method is an adaptation of the ASTM Manual of Water and Environmental
Technology, D1426 Nessler method.
Ammonia is an indication of animal and/or plant decay. It is one of the important
pollutants monitored in natural water due to its toxicity.
The HI97700 photometer is a compact and versatile meter suitable for field or bench
measurements, featuring a:
• Sophisticated optical system
• Meter Validation using Certified CAL Check cuvettes
• Tutorial Mode guides user step‑by‑step
• Auto logging
• Waterproof IP67, floating case
• GLP Features

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Keypad Description
The keypad contains 3 direct keys and 3 functional keys with the following functions:
Press the functional keys to perform the function displayed above them on the LCD.
Press and hold to power off/on. Press briefly to return to the previous screen.
Press to access the menu screen.
Press to display the context‑sensitive help menu.
DESCRIPTION
1) ON/OFF power button 3) Liquid Crystal Display (LCD) 5) Indexing mark
2) Keypad 4) Cuvette holder 6) Battery cover
5.2. FUNCTIONAL DESCRIPTION

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DESCRIPTION
5.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic
radiation and matter. When a light beam crosses a substance, some of the radiation
may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical
path length through the matter and on the physical‑chemical characteristics of the
substance according to the Lambert‑Beer Law:
‑log I/Io= elc d
or
A = elc d
Io= intensity of incident light beam
I = intensity of light beam after absorption
el = molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance
as the other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a
sample and reagent to produce a light‑absorbing compound.
5.3. PRECISION AND ACCURACY
Precision is how closely repeated
measurements are to one another.
Precision is usually expressed as
standard deviation (SD).
Accuracy is defined as the
closeness of a test result to the
true value.
Although good precision suggests
good accuracy, precise results can
be inaccurate. The figure explains
these definitions.
For each method, the accuracy is expressed in the related measurement section.

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DESCRIPTION
Instrument Block Diagram
The internal reference system (reference detector) of the HI97700 photometer
compensates for any drifts due to power fluctuations or ambient temperature changes,
providing a stable source of light for your blank (zero) measurement and sample
measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have
a much higher luminous efficiency, providing more light while using less power. They
also produce little heat, which could otherwise affect electronic stability. LEDs are
available in a wide array of wavelengths, whereas tungsten lamps have poor blue/
violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter,
stronger signal to be received. The end result is higher measurement stability and less
wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from
cuvette imperfections and scratches, eliminating the need to index the cuvette.
5.5. OPTICAL SYSTEM
GENERAL OPERATIONS
6.1. METER VALIDATION: CAL CHECK / CALIBRATION
Validation of the HI97700 involves verifying the concentration of the certified CAL
Check standards. The CAL Check screen guides the user step‑by‑step through the
validation process and user calibration (if necessary).
WARNING: Do not use any solutions/standards other than the HANNA®CAL Check
Standards. For accurate validation and calibration results, please perform these at
room temperature (18 to 25 °C; 64.5 to 77.0 °F).
Note: CAL Check Standards will not read the specified value in measurement mode.
Protect the CAL Check cuvettes from direct sunlight by keeping them in the original
packing. Store between +5 °C and +30 °C (41 - 86 °F), do not freeze.
6. GENERAL OPERATIONS

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GENERAL OPERATIONS
To perform a CAL Check:
1. Press CAL Check key from measurement mode.
The “Not Available“ message or the date/time
and status of the last CAL Check will be displayed
on the screen.
2. Press the Check key to start a new CAL Check.
Press the key at any time to abort the
validation process.
3. Use the keys to enter the certificate value
of the calibration standard found on the CAL Check
Standard Certificate. Press Next to continue.
Note: This value will be saved in the instrument for future validation. If a new
set of calibration standards is obtained please update the certificate value.
4. Insert the A ZERO CAL Check Cuvette A then press
Next to continue. The “Please Wait...“message
will be displayed during the measurement.
5. Insert the HI97700B CAL Check Cuvette B then press
Next to continue. The “Please Wait...“message
will be displayed during the measurement.
6. When the CAL Check is complete the display will
show one of the following messages and the value
obtained during the measurement:
‑ “PASSED“: The measured value is within the accuracy
specification, no user calibration is required.
‑ “OUT OF SPECIFICATION“ and the Calibration key is available: The measured
value is near the expected value. To update the user calibration press Calibrate.
Press Accept to confirm or Cancel to return to the previous screen.

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GENERAL OPERATIONS
6.4. LOGGING DATA/LOG RECALL
The instrument features a data autolog function to help users track of all measurements.
Every time a measurement is made the data is automatically saved. The data log can
hold 50 individual measurements. When the 50 measurements are full and there are no
deleted data, the meter will rewrite the oldest log.
Viewing and deleting the data is possible using the Log Recall menu.
Press the key to enter the menu. Use the functional keys to select Log
Recall and press Select.
6.3. GLP
Press the key to enter the menu. Use the 
keys to select GLP and press Select. Good Laboratory
Practice (GLP) shows the date and time of the last
user calibration (if available) or factory calibration. To
erase the last user calibration and to clear the CAL Check press Clear and follow the
prompts. Press Yes to erase and return to the factory calibration data or No to exit the
clear procedure.
‑ “OUT OF SPECIFICATION“: A user calibration is not
allowed, the measured value is outside of the toler‑
ance window. Check the certified value, expiration
date and clean the outside of the cuvette. Repeat the
CAL Check procedure. If this error continues contact your nearest Hanna Customer
Service Center.
6.2. CHEMICAL FORMULA / UNIT CONVERSION
Chemical formula/unit conversion factors are pre‑programmed into the instrument
and are method specific. In order to view the displayed result in the desired chemical
formula enter menu by pressing and use the keys to select Chemical
Form. Press Select to change the displayed chemical form. Use the keys to
highlight the desired chemical formula and press Select. The selected formula will be
saved when the instruments is powered off.

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Backlight
Values: 0 to 100 %
Press the Modify key to access the backlight intensity.
Use the keys to increase or decrease the value.
Press the Accept key to confirm or the key to
return to the Setup menu without saving the new
value.
GENERAL OPERATIONS
Contrast
Values: 0 to 100 %
Press the Modify key to change the display’s contrast.
Use the keys to increase or decrease the value.
Press the Accept key to confirm the value or the
key to return to the Setup menu without saving the
new value.
6.5. GENERAL SETUP
Press the key to enter the menu. Use the keys to select Setup and press
Select. Use the keys to highlight desired option.
Press No or the key to return to the previous screen.
Press Yes to delete selected log.
Press Del All to erase all the logged data.
If Del All is pressed follow the prompt to confirm.
Press Yes to delete all logged data, No or the key to return to the log recall.
Use the functional keys to highlight a log and press Info to view additional
information about the log. From this screen the Next and Previous keys can be used
to view other logs.
Use the Delete key to erase logged data. After pressing Delete a prompts on display
is asking for confirmation.

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Date / Time
Press the Modify key to change the date/time.
Press the keys to highlight the value to be
modified (year, month, day, hour, minute or second).
Press Edit to modify the highlighted value. Use the
keys to change the value.
Language
Press the Modify key to change the Language. Use
the keys to select the desired language.
Press Accept to choose one of the languages installed.
Date Format
Press the Modify key to change the date format.
Use the keys to select the desired format.
Press the Accept key to confirm or the key to
return to the Setup menu without saving the new
format.
Time Format
Option: AM/PM or 24-hour Press the functional key
to select the desired time format.
GENERAL OPERATIONS
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal
separator. The decimal separator is used on the
measurement screen.
Press the Accept key to confirm or the key to
return to the previous screen.

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Meter Information
Press the Select key to view the model, serial number,
firmware version and selected language. Press the
key to return to the Setup menu.
Restore factory settings
Press the Select key to reset to factory settings.
Press Accept to confirm or Cancel to exit without
restoring the factory settings.
GENERAL OPERATIONS
6.6. REAGENTS/ACCESSORIES
Press the key to enter the menu. Use the 
keys to select Reagents/Accessories and press
Select to access a list of reagents and accessories. To
exit press the key.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a
key is pressed. A long beep alert sounds when the
pressed key is not active or an error is detected. Press
the functional key to enable/disable the beeper.
Tutorial
Option: Enable or Disable
When enabled, the user will be guided step‑by‑step
through the measurement procedure.

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GENERAL OPERATIONS
6.8. BATTERY MANAGEMENT
The meter will perform an auto‑diagnostic test when it is powered on. During this test,
the HANNA®logo will appear on the LCD. After 5 seconds, if the test was successful,
the last method selected will appear on the display. The battery icon on the LCD will
indicate the battery status:
‑ battery full
‑ battery below 10%, replace the batteries soon
‑ battery is low, replace the batteries with new ones
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity.
If a zero reading has been done but not a read, auto‑off time is increased to 30 minutes.
6.7. CONTEXTUAL HELP
The HI97700 offers an interactive contextual help
mode that assists the user at any time.
To access the help screen press the key.
The instrument will display additional information
related to the current screen. To read all the available information, scroll the text using
the keys.
To exit help mode press the or the key and the meter will return to the
previous screen.
7.1. PROPER USE OF DROPPER BOTTLE
(a) For reproducible results, tap the dropper on the table several
times and wipe the outside of the tip
with a cloth.
(b) Always keep the dropper bottle in
a vertical position while dosing the
reagent.
7. PHOTOMETER
PHOTOMETER

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PHOTOMETER
7.2. CUVETTE PREPARATION
Proper mixing is very important for reproducibility of the measurements.
Invert the cuvette a couple of times or for a specified time: hold the cuvette in the
vertical position. Turn the cuvette upside‑down and wait for all of the solution to flow
to the cap end, then return the cuvette to the upright vertical position and wait for all of
the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this
mixing technique is 10‑15 complete inversions in 30 seconds.
This mixing technique is indicated with “invert to mix” and the following icon:
In order to avoid reagent leaking and to obtain more accurate
measurements, close the cuvette first with the supplied HDPE plastic
stopper and then the black cap.
Whenever the cuvette is placed into the measurement holder, it must be
dry outside and free of fingerprints, oil or dirt. Wipe it thoroughly with
HI731318 or a lint‑free cloth prior to insertion.
Shaking the cuvette can generate bubbles in the sample, causing higher
readings. To obtain accurate measurements, remove such bubbles by swirling or by
gently tapping the cuvette.
Do not let the reacted sample stand too long after reagent
is added. For best accuracy, respect the timings described in
each specific method.
It is possible to take multiple readings in a row, but it is
recommended to take a new zero reading for each sample
and to use the same cuvette for zeroing and measurement
when possible.
Discard the sample immediately after the reading is taken, or the glass might become
permanently stained.
All the reaction times reported in this manual are at 25 °C (77 °F). In general, the
reaction time should be increased for temperatures lower than 20 °C (68 °F), and
decreased for temperatures higher than 25 °C (77 °F).

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METHOD PROCEDURE
10 mL
8. METHOD PROCEDURE
REQUIRED REAGENTS
Code Description Quantity
HI93700A‑0 Ammonia Low Range Reagent A 4 drops
HI93700B‑0 Ammonia Low Range Reagent B 4 drops
REAGENT SETS
HI93700‑01 Ammonia LR Reagent ‑ 100 tests
HI93700‑03 Ammonia LR Reagent ‑ 300 tests
For other accessories see page 22.
MEASUREMENT PROCEDURE
Note: If tutorial mode is disabled follow the measurement procedure below. If the
tutorial mode is enabled, press Measure and follow the messages on the screen.
• Fill the cuvette with 10 mL of unreacted sample (up to
the mark). Replace the plastic stopper and the cap.
• Place the cuvette into the holder and ensure that the
notch on the cap is positioned securely into the groove.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and
ready for measurement.
• Remove the cuvette.

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METHOD PROCEDURE
• Add 4 drops of HI93700A‑0 Ammonia Low Range
Reagent A. Replace the plastic stopper and the
cap. Invert 5 times to mix.
• Add 4 drops of HI93700B‑0 Ammonia Low Range
Reagent B. Replace the plastic stopper and the cap.
Invert 5 times to mix.
• Replace the cuvette into the holder and ensure that the
notch on the cap is positioned securely into the groove.
• Press the Read key and the display will show the countdown 3 minutes and 30
seconds prior to the measurement. To skip the timer press Read twice. When the
timer ends the meter will perform the reading. The instrument displays the results
in mg/L of ammonia nitrogen (NH3-N).

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METHOD PROCEDURE
• Press and use the keys to select Chemical Form.
• Press Select to change the displayed chemical form to mg/L of ammonia (NH3)
and ammonium (NH4
+).
INTERFERENCE
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines

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ERROR DESCRIPTIONS
The instrument shows clear warning messages when erroneous conditions appear and when
measured values are outside the expected range. These messages are described below.
No Light: The light source is not functioning properly.
Light Leak: There is an excess amount of ambient light
reaching the detector.
Inverted Cuvette: The sample and the zero cuvettes
are inverted.
Light Low: The instrument cannot adjust the light
level. Please check that the sample does not contain
any debris.
Light High: There is too much light to perform a
measurement. Please check the preparation of the
zero cuvette.
9. ERROR DESCRIPTIONS
Ambient temperature out of limits: The meter is too
hot or too cold for an accurate measurement. Allow
the meter to reach 10 °C to 40 °C (50 °F to 104 °F)
before performing a measurement.
Ambient temperature changed: The temperature
of the meter has changed significantly since the
zero measurement has been performed. A zero
measurement must be performed again.
Out of range: The measured value is outside the limits
of the method.
Battery Low: Battery is low, replace the batteries with
new ones.