HARVARD PHD 4400 Hpsi Programmable User manual

PHD 4400 Hpsi Programmable MA1 70-2200
PHD 4400 Hpsi Remote Programmable MA1 70-2201
PHD 4400
Syringe Pump Series
User's Manual
Publication 5406-001-REV-D

EU Directives WEEE and RoHS
To Our Value Customers:
We are committe to being a goo corporate citizen. As part of that commitment,
we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacte two Directives, the first on pro uct recycling
(Waste Electrical an Electronic Equipment, WEEE) an the secon limiting the use
of certain substances (Restriction on the use of Hazar ous Substances, RoHS).
Over time, these Directives will be implemente in the national laws of each EU
Member State.
Once the final national regulations have been put into place, recycling will be offere
for our pro ucts which are within the scope of the WEEE Directive. Pro ucts falling
un er the scope of the WEEE Directive available for sale after August 13, 2005 will
be i entifie with a “wheelie bin” symbol.
Two Categories of pro ucts covere by the WEEE Directive are currently exempt
from the RoHS Directive – Category 8, me ical evices (with the exception of
implante or infecte pro ucts) an Category 9, monitoring an control instruments.
Most of our pro ucts fall into either Category 8 or 9 an are currently exempt from
the RoHS Directive. We will continue to monitor the application of the RoHS
Directive to its pro ucts an will comply with any changes as they apply.
• Do Not Dispose Product with Municipal Waste
• Special Collection/Disposal Required
WEEE/RoHS Compliance Statement

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Table of Contents
SUBJECT PAGE NO.
General Information:
Manual Description ..............................................4
Warranty ..............................................................4
Repairs ................................................................4
Serial umbers ....................................................4
Calibrations ..........................................................4
General Safety Summary ........................................5
Technical Specifications..........................................6
Theory of Operation ................................................7
Features:
Pressure and Speed ............................................8
Built-in Syringe Table and Custom Syringes ......8
Infusion and Refill Rates......................................8
Target Volume ......................................................8
Auto Fill ................................................................8
Modes of Operation ............................................8
External Connections ..........................................9
onvolatile Memory ............................................9
Stall Detection ......................................................9
Visual/Audible Alarm ............................................9
Power Up Options................................................9
Program Storage..................................................9
User Interface:........................................................10
Description of Keys ............................................11
Entering Data ....................................................12
Special Features Table ......................................12
Initial Setup ............................................................14

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Table of Contents
Operation:
Syringe Loading ................................................15
Running the Pump ............................................16
Diameter ............................................................16
Infuse Rate ........................................................16
Refill Rate ..........................................................16
Target Volume ....................................................17
Auto Fill ..............................................................17
Selecting the Run Mode ....................................17
Pro ram Mode:
Program Description ..........................................19
Entering a Program............................................19
Sequence Operation ..........................................20
Program Printout ................................................23
Program Run Time Error Messages..................23
External Control and Interfaces:
RS-232C Devices ..............................................24
Configuring Pump for RS-232C Devices ..........24
User I/O Devices................................................24
Pro rammin Tutorial:
Multiple Infusion Example..................................26
Ramping Up Infusion Rate Example ................27
Multiple Dispensing Example ............................28
Periodic Dispense Loop Example......................29
Combination of Infusion and Withdraw
Profiles Example ................................................30
Use of Events ....................................................31
Pump Chain Commands:
Model 22 Protocol ..............................................33
Model 44 Protocol ..............................................34
SUBJECT PAGE NO.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Table of Contents
Appendices:
A Table of Syringe Diameters ........................45
B Stainless Steel Syringe ..............................46
C ominal Min/Max Flow Rates ....................48
D Custom Applications ..................................48
E Pressure and Force Specifications ............49
F PHD 4400 Hpsi to PC Connection ............49
G RS-232C Specifications..............................50
H Symphony ..................................................50
I User I/O Connector Specifications ............51
J Accessories and Spare Parts ....................51
K Maintenance and Troubleshooting ............52
L 'Auto-Fill' Valves..........................................53
Fi ure 1 Programmable Model Keypad ..............11
Fi ure 2 Rear Panel and Serial umber Label ..14
Fi ure 3 Fuse Replacement................................15
Fi ure 4 Syringe Loading ....................................16
Fi ure 5 Rear Panel External Connections ........24
Fi ure 6 Multiple Infusions ..................................26
Fi ure 7 Ramping Up Infusion Rate....................27
Fi ure 8 Multiple Dispensing ..............................28
Fi ure 9 Periodic Dispense Loop........................29
Fi ure 10 Comb. Infusion & Withdraw Profiles ....30
Fi ure 11 Use of Events........................................31
Fi ure 12 Use of TTL Signal ................................32
Fi ure 13 PHD 4400 Hpsi to PC Connection ......49
Fi ure 14 RS-232C Specifications ........................50
Fi ure 15 User I/O Connector Specifications ......51
Fi ure 16 'Auto-Fill' Valves ....................................53
FIGURE PAGE NO.
SUBJECT PAGE NO.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
CCAAUUTTIIOONN:: NNoott oorr cclliinniiccaall uussee oonn hhuummaann ppaattiieennttss..
Warranty and Repair Information
MMAANNUUAALL DDEESSCCRRIIPPTTIIOONN
This manual is designed to provide all operational and program information required to
operate and maintain the PH 4400 Hpsi series pumps. The functions and features are
described in the Technical Specifications section.
WWAARRRRAANNTTYY
Harvard Apparatus warranties this instrument for a period of two years from date of pur-
chase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be
defective as to workmanship or materials. This warranty does not extend to damage result-
ing from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends
only to the original consumer purchaser.
I O EVE T SHALL HARVARD APPARATUS BE LIABLE FOR I CIDE TAL OR
CO SEQUE TIAL DAMAGES. Some states do not allow the exclusion or limitation of
incidental or consequential damages so the above limitation or exclusion may not apply to
you. THERE ARE O IMPLIED WARRA TIES OF MERCHA TABILITY, OR FIT ESS
FOR A PARTICULAR USE, OR OF A Y OTHER ATURE. Some states do not allow this
limitation on an implied warranty, so the above limitation may not apply to you.
If a defect arises within the two-year warranty period, promptly contact Harvard
Apparatus, 84 October Hill Road, Holliston, Massachusetts 01746 using our toll
free number 1–800–272–2775, or outside the U.S. call 508-893-8999. Email Address is bio-
[e-mail]. Goods will not be accepted for return unless an RMA
(returned materials authorization) number has been issued by our customer service
department. The customer is responsible for shipping charges. Please allow a reasonable
period of time for completion of repairs or replacement. If the unit is replaced, the replace-
ment unit is covered only for the remainder of the original warranty period dating from the
purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary from
state to state.
RREEPPAAIIRR FFAACCIILLIITTIIEESS AANNDD PPAARRTTSS
Harvard Apparatus stocks replacement and repair parts. When ordering, please describe
parts as completely as possible, preferably using a part number obtained from our
Customer Service department. If practical, enclose a sample part or sketch. We offer a com-
plete reconditioning service.
SSEERRIIAALL NNUUMMBBEERRSS
All inquiries concerning our product should refer to the serial number of the unit, located
on the rear panel (See Figure 2).
CCAALLIIBBRRAATTIIOONNSS
All electrical apparatus is calibrated at rated voltage and frequency. While the flow and vol-
ume will stay calibrated, the peak pressure may vary.
CCAAUUTTIIOONN:: RREEFFEERR TTOO SSEECCTTIIOONN 77,, IINNIITTIIAALL SSEETTUUPP BBEEFFOORREE
PPLLUUGGGGIINNGG IINN PPHHDD 44440000 HHppssii PPUUMMPP..

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
General Safety Summary
Please read the following safety precautions to ensure proper use of your syringe
pump. To avoid potential hazards and product damage, use this product only as
instructed in this manual.
To Prevent Hazard or Injury:
UUssee PPrrooppeerr LLiinnee CCoorrdd
Use only the specified line cord for this product and make sure line cord is certified
for country of use.
GGrroouunndd tthhee PPrroodduucctt
This product is grounded through the grounding conductor of the power cord. To
avoid electric shock, the grounding conductor must be connected to earth ground.
Before making any connections to the input or output terminals of the product, ensure
that the product is properly grounded.
MMaakkee PPrrooppeerr CCoonnnneeccttiioonnss
Make sure all connections are made properly and securely. Any signal wire connec-
tions to the unit must be no longer than 3 meters.
OObbsseerrvvee aallll TTeerrmmiinnaall RRaattiinnggss
Review the operating manual to learn the ratings on all connections.
UUssee PPrrooppeerr FFuussee
Use only specified fuses with product.
AAvvooiidd EExxppoosseedd CCiirrccuuiittrryy
o not touch any electronic circuitry inside of the product.
DDoo NNoott OOppeerraattee wwiitthh SSuussppeecctteedd FFaaiilluurreess
If damage is suspected on or to the product do not operate the product. Contact qual-
ified service personnel to perform inspection.
PPllaaccee PPrroodduucctt iinn PPrrooppeerr EEnnvviirroonnmmeenntt
Review the operating manual for guidelines for proper operating environments.
OObbsseerrvvee aallll WWaarrnniinngg LLaabbeellss oonn PPrroodduucctt
Read all labels on product to ensure proper usage.
Protective Ground
Terminal
CA TION
Refer to Manual

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Specifications
Specifications
Accuracy ±0.35%
Reproducibility ±0.05%
Syringes (Min. / Max.) 0.5 µ l/140 ml
Syringe Diameter 50 mm
Flow Rate:
Minimum 0.0001 µ l/hr (with 0.5 µ l syringe)
Maximum 220.82 ml/min (with 140 ml syringe)
374.39 ml/min (with 50 mm diameter syringe)
Calibration Automatic
Display 2-line, 40-character fluorescent
Memory Nonvolatile (stores all settings)
Interface RS-232C multiplexed dual bidirectional ports
Connectors:
RS-232C RJ11 4-conductor telephone plug
TTL 9-pin D-SUB connector
Linear Force (Max.) 200 lbs
Drive:
Motor 1.8˚ stepper
Control Microprocessor (from 1/2 to 1/32 microstepping)
Step/Revolution from 800 to 12,800
Step Rate:
Minimum 27.3 sec/step
Maximum 416.7 µ sec/step
usher Travel Rate:
Minimum 0.18 µ m/min
Maximum 190.676 mm/min
Timing Belt Drive 2:1
Lead Screw itch 24 threads/in
ower 100-240 VAC, 50/60 Hz, 75 W, 0.75 A fuse
Dimensions, H x W x D 17 x 23 x 29 cm (6.7 x 9.0 x 11.4 in)
Weight 6.4 kg (14 lbs)
Cable Length (max.) 9.1 m (30 ft) for Remote Model only
15 m (50 ft) for RS-232C
9.1 m (30 ft) for User I/O

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Theory of Operation
The PH 4400 Hpsi series employs a microcontroller which controls a small step
angle stepping motor that drives a lead screw and Pusher Block. Microstepping tech-
niques are employed to further reduce the step angle, eliminating flow pulsation. A
keypad is used for entry of operating data to the pump. ata can also be entered via
an RS-232C connector located on the rear panel. The microcontroller, using the inter-
nal Syringe Look Up Table, calculates the cross-sectional area of the syringe selected
and calibrates the flow rate and volume accumulation. The numerous features of the
PH 4400 Hpsi result from the use of microprocessor technology.
The PH 4400 Hpsi Programmable model provides full programmability along with
Infuse/Withdraw capability. Use of the REFILL RATE key, INFUSE/REFILL key, PRO-
GRAM key, AUTO FILL key, Retaining Brackets and Adjustable Stops are all described in
detail in Section 8 of this manual.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Features
PPrreessssuurree aanndd SSppeeeedd
PH 4400 Hpsi can deliver up to 220.82 ml/minute with a single 140 ml syringe.
Maximum pressure is dependent on syringe size.
BBuuiilltt--IInn SSyyrriinnggee TTaabbllee aanndd CCuussttoomm SSyyrriinnggeess
The user may select the syringe to be used from the pump’s built-in syringe table.
Syringes are arranged according to manufacturer and material, and then according to
size. The pump will look up and use the diameter for the syringe you select. (See
Appendix A for a listing of the standard syringes.)
If a syringe is to be used, which is not listed in the syringe table, enter the inside
diameter of the syringe in millimeters.
IInnuussiioonn aanndd RReeiillll RRaatteess
Specify independent rates for infusing and refilling. This allows a slow infusion rate
then a fast refill. If Refill Rate is not set, the software defaults to the set Infuse Rate
value.
TTaarrggeett VVoolluummee
Specify the volume that is to be infused or refilled. The pump will run at the rate spec-
ified until this volume has been delivered when in the Volume mode.
AAuuttoo FFiillll
Auto Fill automatically activates an externally attached solenoid (refer to Appendix L
for part number) and refills the syringe when it is empty. This permits infusions to be
virtually independent of syringe capacity.
MMooddeess oo OOppeerraattiioonn
Pump:
Runs continuously in the infuse or refill directions until stopped.
Volume:
Runs until a specified volume has been pumped or refilled.
Program:
Pump operates according to a specified sequence of instructions.
Note: All modes interact with Auto Fill.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
EExxtteerrnnaall CCoonnnneeccttiioonnss
User I/O
Allows pump operations to be synchronized with external devices or by a person at a
distance from the pump. Connector pins are available to control direction of pump
travel to control an external valve for refilling, and for general use. A simple contact clo-
sure to ground or TTL level signals may be used for inputs. (See Section 10 and
Appendix I.)
RS-232C
Multiple pumps can be 'daisy chained' together and remotely controlled from a com-
puter or any device communicating via RS-232C.
A scale can be connected, enabling the pump to infuse by weight instead of by vol-
ume. (Section 10).
A printer can be connected to record final volumes or weights whenever the pump
stops. In addition the program entered for the program mode can be listed on a con-
nected printer. Both a scale and a printer may be connected simultaneously. (See
Section 10).
RReemmoottee UUnniitt ((CCaattaalloogg NNoo.. MMAA11--7700--22220011 OOnnllyy))
Allows connection to the remote syringe pump unit. The remote unit may be posi-
tioned up to 30 feet away from the control unit.
NNoonnvvoollaattiillee MMeemmoorryy
All operational data entered into the pump from the keypad or from a computer will
be stored, including the program. On power up, the display will blink until the pump
receives its first command and all settings from when it was powered down will be
recalled.
SSttaallll DDeetteeccttiioonn
An optical detector is used to verify expected movement of the motor. If the motor is
prevented from turning due to jamming or excessive back pressure, the pump will
stop and the display will read: PUMP STALLE .
VViissuuaall//AAuuddiibbllee AAllaarrmm
After a power interruption the alarm can be selected to be a silent flashing display or
an audible signal in addition to flashing display. Refer to Section 6 for details on setting
the Alarm Mode.
PPoowweerr--UUpp OOppttiioonnss ((PPuummpp MMooddee OOnnllyy))
Enables the user to select between a standby mode (waiting for a command to begin
running) after power is interrupted or a restart mode that begins running the pump
at the settings that were in process when the power was interrupted. Refer to Section
6 for details on setting the power-up mode.
PPrrooggrraamm SSttoorraaggee
Programmable model can store up to 4 sets of 9 program sequences for later selection.
Features

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
User Interface
The user interface consists of a keypad with a display area The display consists of a 2
line, 40 character alphanumeric vacuum fluorescent display. The display will show one
of three types of messages: efault display, setting display, or an informational message.
The top line of the display is divided into three sections. The first consists of the deliv-
ered volume, or time interval remaining in an operation, expressed with 5 digits plus
a decimal point. Next is the units of the previous number expressed as ‘ml’ for milli-
liters, ‘g’ for grams, if a scale is attached, or ‘time’ for a time interval. Third is the pump-
ing direction, either ‘INFUSE’ or ‘REFILL’, or the current operating state of the pump. A
diamond symbol ◊ in the last character of the display indicates pump chain commu-
nication has been received.
Setting displays are used to facilitate entering control information and data into the
pump. ata entry will be discussed in detail later in this section
Informational messages occur at various times to indicate such items as a data setting
out of range, or a detected problem, such as the pump stalling. Pressing any key clears
the message from the display.
The second line of the display is also divided into 3 sections. The first is the Running
Mode (Pump, Vol, Prog). The second section is the AutoFill indication (no message if
Auto Fill is Off, If On, AutoFill ON is displayed) The third section is a pair of direction-
al arrow heads (< or >) indicating that the pump is running and in what direction.
The keys are used for entering control information and data into the pump. These keys
are grouped into 3 sections (see Figure 1): Set keys, Toggle keys, and ata Entry keys.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Figure 1. Programmable Model Keypad
PPHHDD 44440000
User Interface: Description of Keys
SSeett KKeeyyss
SET – Allows modification of a data item in this group of keys. To modify a data item, press
the relevant key after pressing the SET key. Pressing Set with an appropriate data entry key
will also allow the selection of special features. Refer to EEnntteerriinngg aattaaon page 13.
I FUSE RATE – isplays/sets current infuse rate. Scrolls through rate units with each press
of the key when setting. isplays current programmed rate while running in program
mode.
TARGET VOLUME – isplays/sets current volume mode target volume.
PROGRAM – isplays current program number, program sequence and sequence number.
Held down, with a printer attached and the pump stopped, prints a program listing.
DIAMETER – isplays/sets current syringe diameter. When held down during setting,
accesses built-in syringe table.
REFILL RATE – isplays/sets current refill rate. Scrolls through rate units with each press
of the key when setting.
RS-232C – isplays/sets current RS-232C device(s) attached.
AUTO FILL – Turns Auto Fill feature setting on/off. Also, displays/sets syringe refill volume.
TTooggggllee KKeeyyss
in this group, when permitted, successive states of the keys’ function are selected when
the key is pressed.
RU /STOP – Starts/stops–interrupts running of pump.
I FUSE/REFILL – Changes direction of pusher block travel during operation in Pump and
Volume modes. Pump must be stopped or in Pump Mode to reverse direction of pump.
SELECT MODE – Toggles in an incremental loop through PUMP, VOLUME or PROGRAM
run modes with each press of the key. The current mode is displayed on the 2nd line of the
display.
DDaattaa EEnnttrryy KKeeyyss
1, 2, 3, 4, 5, 6, 7, 8, 9, 0, .– Used to enter numeric data values or access special features.
E TER – Saves and stores displayed data value in memory when setting a data item.

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
User Interface: Entering Data
SSeett KKeeyyss
Keys with rectangular outlines in this group are used to modify or review settings of
the pumps control data. To review the current setting of a control data item, simply
hold down the relevant key and the data setting will appear in the top line of the dis-
play. In the case of the PROGRAM key, if it is held down the data will be sent to the
printer if one is attached.
To modify a data setting, first press then release the green outlined SET key. The dis-
play should then read ‘SET WHAT?’. Press the key in the SET key group whose data is
to be modified. The display will display the current setting. ata is entered into the
pump by either entering a numerical value or by scrolling through a menu of choices.
Always press the green outlined ENTER key to terminate and store each data request
by the pump.
If you are to enter a numerical value, the far left of the display will show ‘ENTER’ fol-
lowed by the units of the number to be entered. Using the numerical keys on the right
side of the keypad (see Figure 1) enter the new data value. Up to five digit numbers
are accepted, including up to four decimal places.
Entering more than five digits will clear the previous five digits entered in the display.
Press the green outlined ENTER key when the desired data value is displayed.
If the far left of the display does not show ‘ENTER’, then a menu of choices is being
displayed. Pressing the relevant key, according to the choices being displayed, selects
successive menu entries. When the desired selection is displayed, press the green out-
lined ENTER key.
If the data value entered is outside the pump’s operating parameters, the display will
read ‘OUT OF RANGE’. Pressing any key will restore the display with the original data
value. Enter another data value within the pump’s parameters or just press the green
outlined ENTER key to reuse the original data value.
The data value entered can be reviewed as described above. Note: Certain data items
have multiple settings. For these, after the ENTER key is pressed, the display will
prompt you for the additional information. Various rules apply to when, what and how
data can be set at various times. See the relevant section for further details.
SSPPEECCIIAALL FFEEAATTUURREESS AACCCCEESSSS TTAABBLLEE
Pressing the green outlined SET key and then the appropriate numerical
key will allow the setting of several special features. Pressing the appropri-
ate numerical key again will scroll between the options provided. Refer to
the table below. When the desired option is displayed, press the green out-
lined ENTER key.
RS-232C Protocol SET 1
Alarm Mode SET 2
Power Up Mode SET 3

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
User Interface: Entering Data
Step Key Setting RS-232C rotocol Description
1 SET SET WHAT? Press and release SET key
Pump Mode
21MODEL 44 PROTOCOL Press and release 1 key
Pump Mode Current RS-232C protocol
displayed
31MODEL 22 PROTOCOL Press and release 1 key to
Pump Mode change RS-232C protocol
4enter XXXXX ml INFUSE Press ENTER to accept +
Pump Mode return to main display
Step Key Setting Alarm Mode Description
1 SET SET WHAT? Press and release SET key
Pump Mode
22ALARM: VISUAL ONLY Press and release 2 key
Pump Mode Current alarm mode
displayed
32ALARM: VISUAL & AUDIBLE Press and release 2 key to
Pump Mode change alarm mode
4enter XXXXX ml INFUSE Press ENTER to accept and
Pump Mode return to main display
Step Key Setting ower-Up Mode Description
1 SET SET WHAT? Press and release SET key
Pump Mode
23POWER UP: STANDBY Press and release 3 key
Pump Mode Current power-up mode
displayed
33POWER UP: RUNNIN Press and release 3 key to
Pump Mode change POWER-UP mode
4enter XXXXX ml INFUSE Press ENTER to accept and
Pump Mode return to main display

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
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1. Read the entire manual to become familiar with all features and functions of the
PH 4400 Hpsi.
2. The operating voltage range for the PH 4400 Hpsi is 100 - 240 VAC, 50/60 Hz.
Use only the specified line cord for this product and make sure line cord is cer-
tified for country of use.
3. Take this opportunity to fill out and mail the Warranty Card. The Catalog and
Serial numbers are located on the Serial Number label on the rear panel. Refer to
Figure 4.
4. Turn on main power switch (see Figure 2a) located directly above the power
cord receptacle on the rear panel. The two-line display will illuminate indicating
that the power connections are correct. The flashing display indicates that power
has just been applied. Pressing any key will reset the blinking display to constant
illumination .
5. See Section 8 for operating Instructions on next page.
6. Fuse Replacement. Turn off power, remove power cord from power module. Use
straight blade screw driver, pry open access door. Remove fuse holder, remove
fuse(s) from holder. Replace fuse(s) and fuse holder. For continued fire protec-
tion, replace fuse only with 250 V fuse of the specified type and rating. (3 AG 0.75
AMP SLO-BLO) (See Figure 3.)
7. Remote Unit Connection (Catalog no. MA1-70-2201 only). Connect the control
unit to the remote pump unit with the cable provided.
Initial Setup
C
Figure 2a. Rear Panel: Power Entry and Serial Number Label
Figure 2b. Remote nit Connection

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Because of the wide range of functions that the PH 4400 Hpsi is capable of per-
forming, certain information about your application must be entered into the pump.
At minimum, the pump needs to know the diameter of your syringe, the infusion rate
and direction of travel.
This is the only information needed to run the pump in the Pump Mode. If not speci-
fied, the refill rate will default to the infuse rate. The pump will need additional infor-
mation to utilize its more advanced features. See Section 6, User Interface, for general
information on data entry.
Operation
CCAAUUTTIIOONN::
UUssee iinn mmaannnneerr nnoott ssppeecciiiieedd bbyy tthhee mmaannuuaaccttuurreerr mmaayy iimmppaaiirr tthhee pprrootteecc--
ttiioonn pprroovviiddeedd bbyy tthhee eeqquuiippmmeenntt..
Figure 3. Fuse Replacement

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
1. The syringe holder and pusher block are fitted with movable retaining brackets
which hold firmly the syringe barrel and plunger when refilling. When loading
the syringe into the pump it is necessary to adjust these brackets. The pusher
block is fitted with a mechanism to release the drive nut from the leadscrew so
that the block can be moved freely to facilitate loading the syringe.
2. Loosen the screws on the syringe block and pusher block to free the retaining
brackets (2 & 3).
3. To free the pusher block from the leadscrew, turn the knob on the front of the
block (1) until the knob slips into the slots or front of the machine.
4. The syringe clamp locking screw on the right side of the syringe block (4) should
be loosened and the clamp rotated to the side.
5. Place the syringe barrel on the syringe holder block and move the pusher block
to accommodate the plunger.
6. Make sure the syringe barrel flange and the plunger flange are held by the retain-
ing clamps. Press the retaining brackets firmly against the flanges and tighten the
retaining screws.
7. Rotate the syringe clamp and press down firmly on the syringe barrel. Secure in
place by tightening the locking screw (4). For syringes greater than 50 cc, it may
be necessary to use the ‘V’-shaped clamp provided. Place clamp over the syringe
barrel and secure to the holder block with the two long thumbscrews.
8. If desired, adjustable stops to limit the syringe plunger travel may be purchased
from Harvard Apparatus (see Appendix J for part number). Install the stops on the
two guide rods at the desired locations.
Operation
124
3
Figure 4. Syringe Loading

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Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Operation
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Pressing the RUN/STOP key starts the pump. The pump will operate according to the rel-
evant data entered as interpreted by the selected run mode. Pressing the RUN/STOP key
while the pump is running stops the pump and the right side of the top line of the display
will indicate ‘INTERRUPT’ plus ‘>’ for infusing or ‘<’ for refilling. This indicates that the
pumping operation has been suspended and can be continued. Pressing the RUN/STOP
key again will continue the pumping operation at the point that it was interrupted.
Changing any of the settings, including the pumping direction and the run mode, cancels
the interrupted operation and resets the display. The pump can also be started and stopped
from a remote source.
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If the inside diameter of the syringe being used is known, enter the value in millimeters.
Otherwise, access the built-in Syringe Table and select the syringe being used. After a new
diameter is entered, directly or via the built-in Syringe Table, the Infuse Rate and Refill Rate
are set to 0 and the Auto Fill feature is turned off. This is done for reasons of safety. The max-
imum diameter that can be entered is 50 mm.
To access the built-in Syringe Table, after pressing the SET key then the IAMETER key, hold
down the IAMETER key for about one second. Once the Syringe Table has been accessed,
each press of the key will scroll through the manufacturers. Find the manufacturer and
material, if applicable, of your syringe. Press the ENTER key to enter your selection. Now,
using the IAMETER key again, scroll by pressing the IAMETER key until the size of your
syringe is displayed, in cc or µL, as indicated on the display. Pressing the ENTER key will
select the size of the syringe and look up and store the diameter. The diameter will be dis-
played until the ENTER key is released. Thereafter, pressing the IAMETER key will display
the selected diameter. In addition, the syringe size selected becomes the default Refill
Volume when the Auto Fill feature is turned on. See Appendix A for a listing of the built-in
Syringe Table and their respective diameters.
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The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes. Also,
the Infuse Rate is used as a starting rate for the program model if one is not specified in the
program, regardless of pumping direction.
When entering the Infuse Rate, the INFUSE RATE key is used to scroll through the allow-
able units of rate. The allowable units are: ml/mn, ml/hr, µl/mn, µl/hr. While running in the
Pump or Volume modes, the Infuse Rate can be changed. If the new rate is valid, it will take
effect when the ENTER key is pressed. The minimum and maximum rates permitted vary
depending on the diameter of the syringe. If an ‘OUT OF RANGE’ message is displayed
when entering a rate, try using a different syringe for your application.
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The Refill Rate is the rate of pumping while refilling in the Pump or Volume Modes or dur-
ing Auto Fill. If the Refill Rate hasn’t been set (rate is 0), the Refill Rate will default to the
Infuse Rate. When entering the Refill Rate, the REFILL RATE key is used to scroll through
the allowable units of rate. The allowable units are: ml/mn, ml/hr, µl/mn, µl/hr. While run-
ning in the Pump or Volume modes, the Refill Rate can be changed. If the new rate is valid,
it will take effect when the ENTER key is pressed. The minimum and maximum rates per-
mitted vary depending on the diameter of the syringe. If an ‘OUT OF RANGE’ message is
displayed when entering a rate, try using a different syringe for your application.

18
Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual
Publication 5406-001-REV-D
Operation
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The Target Volume is the volume that you desire to deliver at the set Infuse or Refill Rate.
The pump must be set for this Volume mode for this feature to work. When entering the
Target Volume, enter the volume desired in mls (milliliters) and press the ENTER key.
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Use AUTO FILL key to toggle between Auto Fill ‘ON’ and ‘OFF’. If Auto Fill is set to ‘ON’, the
pump will next request the volume of the syringe in milliliters. The volume of the syringe
is used as the refill volume of the syringe. This volume can be set to a volume smaller than
the syringe if desired.
When set to ‘ON’, the syringe is assumed to be empty. Auto Fill continuously monitors the
volume of the syringe according to the volume pumped. When the pump determines that
the syringe is empty, the operation in progress is suspended and Auto Fill is activated. The
pumping direction is then reversed and the pump runs at the refill rate. uring the Auto
Fill operation, the display will indicate the volume of liquid in the syringe.
When the volume in the syringe reaches the set refill volume, Auto Fill will stop, and the
previous operation of the pump will resume. Auto Fill continues to monitor the volume of
the syringe. TTL direction output is toggled ‘ON’ during refill. Refill Rate defaults to Infuse
Rate if not set.
Note: Auto Fill will only activate while infusing, (i.e., if the pump direction is set to Refill,
the pump will not stop when the syringe is full.) Also, if the syringe plunger is manually
moved, the pump will lose track of the true syringe volume.
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After entering any necessary operating data into the pump, select the pumping mode that
will be used when the pump is operated. Pressing the SELECT MO E key advances the
second line of the display to indicate the next run mode. Advance the run mode to the
desired mode, either Pump Mode, Volume Mode or Program Mode until it is displayed.
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The pump will continuously pump, infusing or refilling, until stopped. While running, the
Infuse and Refill Rates can be changed. The new rate, for the relevant pumping direction,
takes effect when the ENTER key is pressed. Also, the pumping direction can be changed
by pressing the INFUSE/REFILL key.
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The pump will run, infusing or refilling, until a specified target volume is pumped or
refilled. The TARGET VOLUME key is used to enter the Volume Mode target volume. Used
in conjunction with Auto Fill, the target volume can be greater than the volume of the
syringe. While running, the Target Volume, Infuse and Refill Rates can be changed. The new
rate, for the relevant pumping direction, takes effect when the ENTER key is pressed. Target
Volume can be used to enter a Target weight in grams if a scale is attached via RS-232C.
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In the Program Mode the pump can make complex dispenses including changes in rate
and target volume. These complex dispenses are easily programmed from the keypad and
are detailed in the Program Mode and the Programming Tutorial sections.