HIRAYAMA HV-25 User manual

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Introduction
We would like to express our gratitude for your purchase of our autoclave. This brochure covers
the operation method and a simple maintenance method for the Autoclave
HV-25/50/85/110 you
now own. We hope that owing to your proper handling the autoclave can demonstrate its full
performance and that you
will make regular use of it for a long time.
For optional equipment (0 printer, O floating sensor, 0 cooling fan, automatic water supply
equipment),
if any, read the attached operation manuals for the equipment.
Please check whether or not the product conforms to your order and confirm that it was not
damaged during transportation, Should it be damaged or out of order, please contact the sales
outlet from which you purchased it.
Be sure to send the enclosed warranty registration card to us.
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2
Q (1) No part of this document may be reproduced without permission.
+
Q (2) The contents of this document are subject to change without notice.
Q
2 (3) This document has been carefully compiled. If you have any questions or necessary
t
$ information uncovered in the document, please contact us or the sales outlet from which
?
$ you purchased the product.
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Table of Contents
Date Descriptionof Revision
Jun. 11, 1996
Additions and revisions to WARNING, CAUTION and NOTE.
Instructions under "Replacing Lid Packing" in Chapter
4: Maintenance and Service revised.
Instructions for "Operation with
a WaSte Disposal Bag" added.
Instructions for "Successive Operations with the Product" added.
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Before Using
In this manual the following headwords are applied to items to which great attention should be
given:
WARNING: Precaution indicating an imminent dangerous situation which if not avoided
may lead to death or serious injury.
a CAUTION: Precaution indicating a dangerous situation which if not avoided may lead to
moderate or slight injury.
IMPORTANT: Indicates items you are strongly advised to obey.
NOTE:
Items you should pay attention to during operation.
r
/(\ WARNING
Never use the autoclave to sterilize the following dangerous objects or substances containing
alkali content. Sterilization of such objects may cause explosion, corrosion of the working
chamber or piping, and deterioration in packing.
/ List of Dangerous Objects:
(1) Explosive substances
Nitroglycol, nitroglycerin, nitrocellulose, and other explosive nitric esters.
*Trinitrobenzene,trinitrotoluene, picric acid, and other explosive nitro compounds.
*Peraceticacid, methyl ethyl ketone peroxide, benzoyl peroxide, and otherorganicperoxides.
(2) Ignitable substances
*Metallic lithium, potassium, sodium, yellow phosphorous, phosphorus sulfide, and red
phosphorus
Celluloids, calcium carbide (carbide), lime phosphide, and magnesium powder
Aluminumpowder, magnesium powder, and metallic powders otherthan aluminum powder
*Sodium.dithionite(or sodium hydrosulfite)
(3) Oxidizer
*Potassiumchlorate, sodium chlorate, ammonium chlorate, and other chlorates
Potassium perchlorate, sodium perchlorate, ammonium perchlorate, and other perchlorates.
*Potassiumperoxide, sodium peroxide, barium peroxide, and other inorganic peroxides
*Potassiumnitrate, sodium nitrate, ammonium nitrate, and other nitrates
Sodium chlorite and other chlorites
Calcium hypochlorite and other hypochlorites
(4) Inflammable substances
Ethylether, gasoline, acetaldehyde, propylene oxide, carbon disulfide, and other substances
whose flash points range from
-30°C to 0°C exclusive.
*Methanol,ethanol, xylene, benzyl acetate (or amyl acetate), and other substances whose
flash points range from
0°C to 30°C exclusive.
Kerosene, gas oil,turpenineoil, isopentyl alcohol (orisoamyl alcohol), acetic acid, and other
substances whose flash points range from
30°C to 65°C exclusive.
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(5) Harnmable gas (hydrogen, acetylene, ethylene, methane, ethane,propane, butane, and other
substances that are
gas at a temperature of 15°C at 1atmospheric pressure.)
Before opening the lid, be sure to check that the pressure gauge reads "0 kgf/cm2".
I 0 Never modify the product.
- A CAUTION
Foreign matter (metals, liquid) may enter through the vent hole. Operating the equipment
with such foreign matter inside may cause trouble with the equipment, fire and electric
shock.
Do not forcibly bend, twist, tie or extend the power cord. Do not place heavy objects on the
cord.
A damaged cord or exposed wire may cause fire orelectric shock.
Never connect the power cord to a power supply other than the voltage that is rated.
Connection to such a power supply may cause fire or electric shock.
If not plugging the sterilizerinto a grounded socket, ground the equipment separately before
connecting it to a power source.
Never ground to a gas pipe or vinyl chloride water servicepipe.
Before closing the lid, check to see that there are no foreign matters in the portion of the lid
which is in contact with the lid packing. Foreign matters may cause steam leakage.
Whenever a bag, such as a waste disposal bag, isused for sterilization,put it in the wire mesh
basket and place the
basket in the working chamber. If the bag is placed directly in the
working chamber, the piping may become cloggedby itand overheating, overpressurization,
and/orlack of water
may result.
,
Be careful not to pinch hands when closing the Iid.
Do not put your face or hands close to the working chamber when lifting the lid after
operations are complete; steam will gush out of the opening of the chamber.
The lid, working chamber, packing and panel are extremely hot immediately after the
completion of operation. Do not touch the equipment or you may
get burned.
Put on heat insulation gloves before removing a substance from the working chamber. Do
not put your hands into the chamber until the steam has been vented.
@ It takes a lot of timefor the liquid to cool. Be sureto check that the temperature has dropped
sufficiently before unloading the liquid from the working chamber, or you may get burned.
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Do not unload the exhaust bottle or drain the working chamber when the chamber is under
pressure. Boiling water or steam may gush out, and
you may get burned.
a Do not unload the exhaust bottle before water in the bottle has been sufficiently cooled.
0 Do not dispose of used batteries in fire; they may explode.
a If you encounter any anomaly (e.g. abnormal sound and smell, smoking), immediately shut
off power. After checking to see that the abnormal condition is not continuing, call us or our
authorized sales outlet from shich
you purchased it.
0 If the display differsbetween the steps, turn off the POWER switch, and then turn the power
on again.
If the condition still remains, turn off the power and call us or our authorized sales
outlet from which
you purchased it.
a If the equipment is installed in a place which is 800 m or higher than sea level (i.e. under low
pressure
in mountain areas), the over pressure preventive device and the air vent device
require changes to their setting.
In this case, be sureto contact us. Do not use theequipment
before changing the setting.
a In transporting the equipment, put the lid on and slide the open/close lever to the end of the
close side to prevent the lid from opening.
When moving the lid, do not hold it by the handle, otherwise the lid may become difficult to
close.
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How to Read This Manual
a This operation manual consists of the following sections covering the information required for
proper operation ofthe autoclave
HV-25/50/85/110:
Chapter 1. What IsAutoclave HV-25/50185/11O?"
This section describes the uses and features of the product and the names and functions of its
parts.
Chapter 2. Installation
This sectionexplains where the equipment should be installed and how to install it. The product
incorporates precision parts. Be sure to follow the instructions covered in this document.
Chapter 3. Operation Method
This section illustrates how to change various set values, and describes operation before starting
the equipment and after automatic operation. This section also covers the display and action of
the equipment during automatic operation.
Chapter 4. Maintenanceand Servicing
This section explains the methods for draining water from the exhaust bottle or working charn-
ber, servicing the body, and parts replacement.
Chapter 5. Specifications
his section includes dimensions, power consumption, working range, piping and wiring dia-
grams of the product. Refer to this section as is required.
Chapter 6. Inthe Event of Suspected Failure
This section covers troubleshooting procedures for the product. If you encounter anything sus-
pected to be due to failure, read this section first of
all.
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Contents
Introduction ................... .. ............................................................................................
Table of Contents ......................................................... ....................................
BeforeUsing ...............................................................................................................
How to ReadThis Manual ................... .... .................................................................
Contents .................... ... ..............................................................................................
...........................................................
Chapter 1 . What Is Autoclave HV-25/50/8511lo?
.................................................................................................................................
1 . Product Uses
2
. Product Features ..........................................................................................................................
3 . Name and Functions of Each Part .............................................................................................
Chapter 2 . Installation ................... ... .......................................................................
1 . InstallationInstructions ........................... ....................................................................................
2 . InstallationProcedure ................................................................................................................
Chapter 3 . Operation Method ................... ...... .........................................................
Basic Operation Method ...........................................................................................................................
Turning "ON" POWER Switch ....................................................................................................
PouringWater ...............................................................................................................................
Loading Substance .......................................................................................................................
Selecting Mode (Process) ............................................................................................................
Changing Set Values (RegisteringValues by Customer) .............................................................
Checking andCorrectingthe ClockTime ................................... .... ...............................................
Checking and Setting of Turn-onTimer ......................................................................................
StartingOperation ........................................................................................................................
Unloading ......................................................................................................................................
10 . After Completionof Operations ..................................................................................................
11 . To Interrupt Operation ................................................................................................................
12 . If Power Supply Is Cut Off during Operation ................... .. .......................................................
13 . Operatjonof Cycles ................... .. .......................................................................................
Chapter 4 . Maintenance and Service .............................................................................
1 . DrainingExhaust Bottle ................... A ....................................................................................
2 . DrainingWorking Chamber ........................... ............ ....................................................................
3 . Cleaning Working Chamber .......................................................................................................
4 . Body Service ................................................................................................................................
5 . ReplacingLid Packing ...................................................................................................................
6 . Replacing Backup Battery ........................................................................................................
Chapter 5 . Specification ................... .. .....................................................................
Chapter 6 . Inthe Event of SuspectedFailure .................................................................
1 . Error Detection (Alarming)Operation ................... .. ...... .. ......................................................
2 . EarlyTrouble Shooting .................................... .... ..........................................................................
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Cha~ter 1. What Is Autoclave HV-25/50/85111O?
1. Product Uses
This product is designed for research use only (Modes 1 - 3). It is not labeled or marketed
for use in medical or clinical applications.
This product is also used to liquefy media (Mode 4). Mode 4 can also be used to prewarm the
chamber for a faster start.
2. Product Features
This product is equipped with a cover for the lid that may be heated at hlgh temperature during
use.
Theproduct incorporates an automatic timer which permits you to begin operation at any desired
time within a period of one week.
The product is provided with a variety of modes of operation. The modes are divided into two
broad categories: Sterilization-warming mode and liquefaction mode.
If you do not take out the
sterilized medium immediately, then select the sterilization-warming mode, and coagulation is
prevented. To liquefy a coagulated medium, use the liquefaction mode.
This autoclavecan be set to automatically exhaust % inside the chamberat a desired rate (exhaust
valve aperture) after sterilization is over.
Optional equipment (printer, floating sensor, cooling fan, automatic water supply equipment)
can be installed onto the product at a later date.
3. Name and Functions of Each Part
Outer View of Body
When the lid is open
Lid nT Magnetcatch
!I:er switch
- % Pressure gauge
Front View
Port for attaching
printer
Exhaustbottle
-,..-
El- Drain port,
Water receiver Panel
Lid gasket Workingchamber
\
Comer plate I
Vent hole
arrying handle
Side View
11
(2 pieces for HV-85
Exhaust port'
lo) Rear View
fa
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Display and Operation Switches
@ Digital Display (Temperature, Time,ofDay, Error)
The digital display indicates set temperature, current time and pre-selected time of day when the
equipment is in a standby state and it indicates temperature in the working chamber during
operation.
If any troubIe occurs in the equipment and an error is detected, the display indicates
the error.
@ Digital Display (Time, Time of Day, Exhaust %)
The digital display indicates set time, set exhaust %, current time, and pre-selected time of day
when the equipmentisin a'standbystate and itindicatesremaining time before the completion of
sterilization.
@ Unit Display ("C, HOUR, MONTH, MINUTE, DAY, EWHT %)
A unit corresponding to a current digital display illuminates.
@ Step Display (ST-BY, HIEATG, $TIER., EXWT., COOL,WARM, COMP.)
-
All the steps included in a selected modeilluminate,and the current step flickers. (COOLdisplay
is illuminated when the optional equipment has been installed.)
6) MODE Display (ILIQ, SOILID, AGAR)
The purpose of the selected mode Lights up.
@ FUNC. Display (TIMIER,CLOCK, PRINT)
The
TIIMIER display illuminates when you set the turn-on timer or check the contents of its
setting, and flickers when you complete setting the timer. The
CLOCK display lights up when
you check or changethetime, and flickers when the backup battery has gone dead. (The
PRIINT
display illuminates when the optional equipment has been instalIed.)
a MODE Switch
This switch is used to select the mode and to check
a set temperature or time, or exhaust 5%.
8 FUNC. Switch
This switch is used to check the set values for each function.
@ SET VALUE INCREASEDECREASE Switches ( A,
)
These switches are used to increase or decrease set values.
@ SETENTSwitch
This switch is used to change a set value.
0 NEXT Switch
This switch is used to select the item for which you wish to change the setting.
@ START/STOP Switch
This switch is used to start or stop operation.
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Chapter 2. Installation
- A CAUTION
If the equipment is installed in a place which is 800 m or higher than sea level (i.e. under low
pressure in mountain areas), its specifications require to be changed. In this case,
be sure to
contact
us. Do not use the equipment before changing.
In transporting the equipment, put the lid on and slide the opedclose lever to the end of the
close side
to prevent the lid from opening.
When moving the lid, do not hold it by the handle, otherwise the lid may become difficult to
close.
1. InstallationInstructions
O Avoid installing the equipment in a place where
its body may be exposed to water or chemicals, or
where corrosive and explosive gases may
be
produced nearby.
Q Avoid installing the equipment in a place which is
exposed to high humidity, direct sunlight or much
dust.
O Avoid placing the equipment directly under a fire
detector. If you open the lid immediately after
completion of operation, steam comes out of the
working chamber, and may activate the detector.
@ Arrange the equipment with a clearance of 10 cm or
wider on the right side and
12 cm or wider on the rear
sideto prevent the vent hole fiom being blocked.
I 2 cm or over 1
10 cm or over
O Avoid installing the equipment with its rear side
located near outlets or electrical appliances as steam
comes out of the exhaustport on the rear.
@ Avoid an installation place which is subject to impact
orvibration.
6 Place the unit in a level, fm place.
@ Avoid installing in a place which is subjected to a
room temperature of 5°C or below or 35OC or above.
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2, Installation Procedure
@ Put the body on the caster stoppers to prevent the body from ac
Anchor the body as described in the following.
(1) Set the stopper the specified distance from walls.
Specified distance
L = HV-25 .............. 49 cm or over
HV-50 .............. 55 cmor over
Push ,,
HV-85/110 ....... 67 cm or over
(2) Push the body until the front casters roll onto the stopper.
@ Connect the power cord to a rated power supply.
Reliably ground the earth cable.
w
a WARNING
Do not forcibly bend, twist, tie or extend the power cord. Do not place heavy objects on the
cord.
A damaged cord or exposed wire may cause fire or electric shock.
Never connect the power cord to a power supply other than the voltage that is rated.
Connection to such a power supply may cause f~eor electric shock.
0 If not plugging the sterilizer into a grounded socket, ground the equipment separately before
connecting it to a power source.
0 Never ground to a gas pipe or vinyl chloride water service pipe.
Connect the power cord to a grounded electrical outlet.
Make sure that the outlet voltage corresponds to the voltage ofthe autoclave (120 VAC or
20812201240 VAC, 50160Hz single phase). Check the label on the instrument or the tag on
the power cord for the proper voltage requirements.
If the plug does not fit the outlet, get an electricianto change the outlet receptable or ask him
to recommend a suitableplug. The black wire ofthe power cord is the "hot" wire.
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@ Pour water into the exhaust bottle.
Add water to the exhaust bottle as described below.
Drainpo
Pol
Reference
line level
(1) UnIoad the exhaust bottle from the body.
Pull the bottle outwards until the top handle can be grabbed
securely. Lift the bottle out of the autoclave using this handle.
(2) Pour water into the bottle through the water filling port. Fill
water to the reference line level.
LOW level
1 Drain valve
(3) Checkto make sure that thewater level is at the LOW level (the
lowest water level).
If too much water has been poured in, then place the bottle in a
level sink with the side of the water filling and drain ports facing
downwards. Any excessive water is drained automatically until
the water level is lowered to the
LOW level.
(4) Check to see that the drain valve, located at the bottom of the
exhaust bottle housing
area, is closed.
(5) Load the bottle into the area.
Besureto pushthebottle to the end,orelse an error ( E , E ) will
occur.
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@ Referring to "chapter 3. Operation Method," open the lid and take out the accessories.
@ Place the bottom place in the working chamber.
@ When the product is operated continuously, steammay be emitted from the exhaust pon located
at the rear of the body. In this case connect one end of the attached exhaust hose to the exhaust
port
and place the other end in a container.
Discard water collected in the container to prevent the end of the exhaust hose from becoming
immersed in water.
Bottle orsimilar item
(for receivingsteam)
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Chapter 3. Operation Method
Basic Operation Method
Turning "ON' PowerSwitch 1
See '1. Turning 'ON' POWER Switch" on page 8.
v
Open the lid.
I1
v
See '2. Pouring Water" on page 8.
Pourwater.
v
i
Loadsubstance.
11
v
A- See '3. LoadingSubstance" on page 9.
1 Put the lid down.
i
Check andselect mode.
- See "4. Selecting Mode (Process)" on page 10.
I
Check and change set values. 1 --------- See '5. Changing Set Values" on page 12.
I
I
i i
Whenstarting operation by Turn-onTimer
I Start operation. 1 See "8. StartingOperation" on page 19.
1
I Set Tum-on Timer.
Check
if operation is completed.
I
v
1 Openthe lid.
I
See "9. Unloading"on page 21.
v
Unloadsubstance.
v
See "7. Checking and Setting of Turn-onTimer" on page 17.
Turn 'OFF' powerswitch. I --------- See "10. After Completion of Operation'' on page 21.
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1. Turning "ON" POWER Switch
O Turn ON the POWER switch at the front of the body.
When the openiclose lever is set to "LOCK" (left side), settings light up on the display. The
autoclave is ready in this state.When the open/close leveris setto
"UNLOCK" (anywhere other
than on the left side), " L
is shown on the display.
Ready state
2. Pouring Water
, <r>. IMPORTANT
0 In operation of UNLOCKLOCK lever, never fail to put POWR switch ON.
a Slide the open/close lever to the UNLOCK side (the right end).
@ Grab the handle, and lift the lid as shown in the figurebelow.
@ Pour water through the opening of the working chamber until you can see water through the
hole at the center of the bottom plate.
The HV-25 requires 1.5 liters ormore of water; the HV-50,2liters ormore; theHV-85,4liters or
more; and the HV-110.5 liters or more. It is also required to pour water forMode
4 (AGAR).
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3. Loading Substance
I
Be careful not to pinch hands when closing the lid.
Check to see that the area of the lid that is to come in contact with the lid gasket is free from
foreign matter before closing the lid. Foreign matter present in the area may cause the steam
leakage.
a Whenever abag, such as a waste disposal bag, is used for sterilization,put it in the wire mesh
basket and place the basket in the working chamber. If the bag is placed directly in the
working chamber, the piping may become clogged by it and overheating, overpressurization,
andorlack of water may result.
A IMPORTANT
0 Keep the lid open for 15 minutes or more between operations when the equipment is operated
continously. Check to see that the temperature in the working chamber is
50(C or below
before starting the next operation (operating the opedclose lever).
Be sure to use the bottom plate.
-
@ Place the substance to be sterilized into the
working chamber.
@ Press the lid down until the magnet catch is
attracted to the magnet.
@ Slide the opedclose lever to the LOCK side
(the left end).
Magnet catch
3
y A NOTE
a When sterilizing an empty deep container, lay it on its sidein the working chamber sothat it
will be permeated with steam. An upright position may cause insufficient sterilization.
If a waste disposalbag is used in sterilization, open the bag far enough that the bag is not in
contact with the inside surface of the working chamber. Insufficient sterilization
may be
caused if the bag is closed during sterilization. When thebag is opened excessively,steam is
prevented fromcirculating
in the working chamber. This may also result in insufficient
sterilization.
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Do not pile specimens on top of one another. When the chamber is overly packed, steam fails
to penetrate
to all points, resulting in incomplete sterilization.
0 In sterilizing liquids such as chemical and medium, pay attention to the quantity of the liquid
in relation
to its container. For an Erlenmeyer flask, the amount of chemical should be approx.
314 of the capacity of the container; for a test tube, the appropriate quantity of chemical is
approx. half of the capacity of the container. Toomuch chemical may result in overflow from
the container during the temperature rising or coolingprocess.
When using a container, loosen the cap or use a breathable cap. An unbreathable cap may
cause the container to burst.
In the caseof liquefaction of agar medium, its quantity should be 2 liters or less per container.
Two liters
or more of agar medium ,maynot be completely liquefied.
4. Selecting Mode (Process)
The following modes are programmed in the microcomputer. Select a mode suitable for your
purpose.
O Press the MODE switch.
Each time you press the switch, the current mode repeatedly changes from
\. /
Mode 1 to Mode 2, 3,4, 1, ... in this order.
Mode
1
2
Application
Sterilizationof
agar medium (warmed for the prevention of coagulation after sterilization).
Sterilization of liquid, such as water, medium, reagent, liquid medicine, that withstand
high
3
temperature,high pressure steam.
Sterilization of tools of glass, ceramic, metal or rubber
that withstand high temperature, high
4
pressure steam and abrupt de pressurization during an exhaust process.
Liquefaction of medium.
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Mode
1
Mode
2
Mode
3
Mode
4
Initial Set Value
XI-J-
Step Display
WEATG -+ STER. -+
EXHT. (Varied) -+WARM
Step Display
BEATG -+ STEW. -+ EXHT. (Varied)
Alternate display
IVIUUC
Display
ILJQ
Sterilization
Temperature
12 1 OC
I
NOTE: Lid can only be opened at approximately 3O below boiling
point.
Mode
~i~~l~~
L~Q
Initial Set Value
Mode
~i~~la~
XDLD
Step Display
HEATG -+ STER. -+ EXHT. (Fixed)
Sterilization
Time
20 minutes
Mode
Display
AGAR
Step Display
WEATG WARM
Exhaust %
10%
Initial Set Value
Warming
Temperature
50 "C
Warming
Temperature
/
Sterilization
Temperatul~
121OC
Sterilization
Temperature
121OC
Initial Set Value
Exhaust
%
10%
Sterilization
Time
20 minutes
Sterilization
Time
20 minutes
W~rming
Exhaust %
Temperature
50 OC
Sterilization
Temperature
100
OC
Sterilization
Time
10minutes
Warming
Temperature
/
Exhaust %
/
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5. Changing SetValues (Registering Values by Customer)
To change setvalues (sterilization temperature, sterilization time, warming temperature, opening
of exhaust valve, liquefaction temperature, liquefaction time), follow the steps below: Settings
cannot be changed while the chamber is running except if wanting to change the exhaust rate
while exhausting the chamber. For details on changing exhaust rate while exhausting the
chamber, see
"Exhaust % during the Valve Opening Variable Exhaust Cycle" on pagel 8.
O Press the SETIENT switch.
The display of a set sterilization (liquefaction) temperature starts
flickering. This indicates that the set value is now changeable.
O Press the NEXT switch to select an item the setting of which you desire
to change.
* Each time you press the switches, the changeable set items change
repeatedly as follows:
[mi W]
Switch Operation NEXT
O Change the displayed value using the setting increaseldecrease switches
(A, v)-
Each time you press the switches, the displayed value increases or
decreases as follows:
Sterilization temperature:
In increments of
1°C within a range of:
HV-25 105 - 126°C
HV-50 105 - 135°C
EN-85
105 - 135OC (105 - 128OC)*
HV-110 105 - 135OC (105 - 123OC)*
* Specification for particular area
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