HORIBA ABX PENTRA 80 RAA022AA User manual

Technical Manual
P/n: RAA022B

PENTRA 80 TECHNICAL MANUAL RAA022BA
2/4
1. Revisions
This document applies to the latest higher software version.
When a subsequent software version changes the information in this document, a new issue will be
released.
1.1. Notice of liability
The information in this manual is distributed on an ’’as is’’ basis, without warranty. While every
precaution has been taken in the preparation of the manual, ABX DIAGNOSTICS shall have any liability to
any person or entity with respect to liability, loss, or damage caused or alleged to be caused directly or
indirectly by the instructions contained in this manual or by the computer software and hardware
products described herein.
1.2. Trademarks
Other product names mentioned within this publication may be trademarks or registered
trademarks of other companies.
1.3. Copyright ®2002 by ABX DIAGNOSTICS
All rights reserved. No part of this book may be reproduced or transmitted in any form or by any
means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written
permission of ABX DIAGNOSTICS.
ABX DIAGNOSTICS
Parc Euromédecine
Rue du caducée
B.P. 7290
34184 MONTPELLIER Cedex 4 - FRANCE
Tel: (33) (0)4 67 14 15 16
Fax: (33) (0)4 67 14 15 17
Table 1: Table of Revisions
Index P/N revision Revision Section Date
RAA022AA RAH912AA <V1.01 All 31/03/02
RAA022B RAH986 XL 80 integration All 30/04/04

INTRODUCTION
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2. Working conditions
2.1. Environment
The Pentra 80 should be operated in an indoor location only. Operation at an altitude over 3000
meters (9800 feet) is not recommended. Instrument is designed to be safe for transient voltages
according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.
Please ask your ABX Diagnostics representative service center for any information about the operating
location when it does not comply with the recommended specifications.
2.2. Location
The Pentra 80 should be placed on a clean and leveled table or work station. Please note that the
Pentra 80, printer and reagents weigh approximately 40 kilograms (88 lbs). Avoid exposure to sunlight.
Proper ventilation requires that a space of at least 20 cm (8 inches) must be left behind the instrument.
2.3. Grounding
Proper grounding is required. Check that the wall ground (earth) plug is correctly connected to the
laboratory grounding electricity installation.
If there is no ground then use a ground stake. Current electricity Standards must be applied.
2.4. Humidity and temperature conditions
The Pentra 80 must function between 16 to 34°C (61 to 93°F). Maximum relative humidity 80% for
temperatures up to 31°C (88°F) decreasing linearly to 50% relative humidity at 40°C (104°F). If it is kept
at a temperature of less than 10°C (50°F), the instrument should be allowed to sit for an hour at the
correct room temperature before use.
2.5. Electromagnetic environment check
The Pentra 80 as been designed to produce less than the required level of electromagnetic
interferences in order to operate in conformity with its destination. The electromagnetic interferences
caused by the Pentra 80 are limited to a level allowing the correct operation of other instruments in
conformity with their destination.
In case of problems, check that the instrument is not placed in proximity of electromagnetic fields,
or short wave emissions (radars, X-rays, scanner, etc...).
2.6. Environment protection
Used accessories and consumables must be collected by a laboratory specialized in elimination
and recycling of this kind of material according to the legislation.

PENTRA 80 TECHNICAL MANUAL RAA022BA
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Specifications
Section 1: Specifications
1. Technical specifications ..........................................1-2
1.1. Parameters................................................................... 1-2
1.2. Throughput Analyses ................................................... 1-2
1.3. Tube identification ...................................................... 1-2
1.4. Reagents...................................................................... 1-3
1.5. Internal Computer ....................................................... 1-3
1.6. Measurements and computation .................................. 1-3
2. Physical specifications.............................................1-3
2.1. Power requirements..................................................... 1-3
2.2. Operating temperature and humidity ........................... 1-3
2.3. Dimension and weight ................................................ 1-3
2.4. Minimum specimen volume ........................................ 1-4
2.5. dilution ratios .............................................................. 1-4
2.6. Hgb measurement ....................................................... 1-4
2.7. Counting aperture diameters........................................ 1-4
2.8. Reagent consumption (ml) ........................................... 1-4
3. Summary of performance data.................................1-5
3.1. Repeatability ............................................................... 1-5
3.2. Linearity ...................................................................... 1-5
3.3. Carryover .................................................................... 1-5
4. Reagents specification .............................................1-6
4.1. ABX DILUENT............................................................. 1-6
4.2. ABX LYSE .................................................................... 1-6
4.3. ABX BIOLYSE .............................................................. 1-6
4.4. ABX CLEANER............................................................. 1-7
4.5. ABX EOSINOFIX.......................................................... 1-7
4.6. ABX BASOLYSE II........................................................ 1-7
4.7. Waste handling precautions ........................................ 1-8
5. Limitations ..............................................................1-8
5.1. Maintenance ............................................................... 1-8
5.2. Blood specimens ......................................................... 1-8
5.3. Known interfering substances

PENTRA 80 TECHNICAL MANUAL RAA022B
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1. Technical specifications
1.1. Parameters
1.2. Throughput Analyses
• 80 samples per hour.
1.3. Tube identification
• By mean of Keyboard, internal and external Barcode.
Table 1: CBC Mode
WBC White Blood Cell
RBC Red Blood Cell
Hgb Hemoglobin Concentration
Hct Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red Distribution Width
PLT Platelets
PDW Platelets Distribution Width
MPV Mean Platelet Volume
PCT Plateletcrit
Table 2: CBC + 5DIFF Mode
WBC White Blood Cell
LYM Lymphocytes % and #
MON Monocytes % and #
NEU Neutrophils % and #
EOS Eosinophils % and #
BAS Basophils % and #
LIC Large Immature Cell % and #
ALY Atypical Lymphocytes % and #
RBC Red Blood Cell
Hgb Hemoglobin Concentration
Hct Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red Distribution Width
PLT Platelets
PDW Platelets Distribution Width
MPV Mean Platelet Volume
PCT Plateletcrit

SECTION 1 SPECIFICATIONS
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1.4. Reagents
• ABX DILUENT (20 Litres).
• ABX CLEANER (1 Litre, Integrated).
• ABX EOSINOFIX (1 Litre, Integrated).
• ABX BASOLYSE (1 Litre, Integrated).
• ABX LYSE (0.4 Litre, Integrated).
1.5. Internal Computer
• Color LCD touch screen: 12 inches.
• Industrial PC board:
.................................Windows NT 4.0. for Pentra 80
................................... Windows XP for Pentra XL80
• Processor frequency ................ Celeron 433 MHz.
• Memory capacity:
................................................128 Mo for Pentra 80
............................................256 Mo for Pentra XL80
• Hard drive ................................................... 20 Go.
• Floppy disck.
• CD ROM drive.
• RS 232C
• USB, Ethernet for Pentra XL80.
• Keyboard.
• Mouse.
1.6. Measurements and computation
• Impedance for Wbc, Plt, Rbc, Baso.
• Photometry for Hgb.
• Impedance and light scattering for Lym, Mon; Neu, Eos, Aly and Lic.
• Computation from stored data that was directly measured for Hct, Mcv, Mch, Mchc, Rdw, Mpv,
Pct and Pdw.
2. Physical specifications
2.1. Power requirements
• Power supply..................from 100 Vac to 240 Vac.
................................. 50 Hz to 60 Hz.
• Power consumption .................Maximum 230 VA.
• Printer ...................................... Depends of printer.
.............................................. (See printer’s manual).
2.2. Operating temperature and humidity
• 16 - 34°C (61-93°F) room temperature.
• Maximum relative humidity 80% for temperature up to 31°C (88°F) decreasing linearly to 50%
relative humidity at 40°C (104°F).
2.3. Dimension and weight
• Dimensions.................................. 82 x 57 x 54 cm.
........................... 34.1 x 23.3 x 22 in.
• Weight.......................................................... 55 Kg.
............................................. 122 lbs.

PENTRA 80 TECHNICAL MANUAL RAA022B
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2.4. Minimum specimen volume
• CBC Mode (CBC) ...........................................30µl.
• CBC + 5DIFF Mode (DIF)................................53µl.
2.5. dilution ratios
• WBC/BASO.................................................. 1/200.
• LMNE ............................................................. 1/80.
• RBC/PLT .................................................. 1/10000.
• HGB ............................................................. 1/250.
2.6. Hgb measurement
• Hgb chamber, LED 555 nm.
• Modified Drabkin method (cyanmethemoglobin).
• Light source .................. Electroluminescent diode.
• Wavelenght .................................550nm +/- 10nm.
2.7. Counting aperture diameters
• WBC/BASO...................................................80µm.
• LMNE ............................................................60µm.
• RBC/PLT .......................................................50µm.
2.8. Reagent consumption (ml)
Table 3: Reagents consumption
Cycles Estimated
duration (s)
Diluent
(ml)
Eosinofix
(ml)
Basolyse II
(ml)
Cleaner
(ml)
Lyse
(ml)
CBC/DIFF 0’45’’ 27.4 1.0 2.0 1.0 0.45
CBC 0’45’’ 24.4 -2.0 1.0 0.45
Prime DILUENT 3’00’’ 44 - - - -
Prime
EOSINOFIX 1’34’’ 1.6 23.7 - - -
Prime
BASOLYSE 2 1’25’’ 1.7 -23.7 1.0 -
Prime CLEA-
NER 1’24’’ 1.7 - - 24.7 -
Prime LYSE 1’31’’ 2.7 - - - 8.4
Prime ALL 7’13’’ 50.7 24.0 24.0 25.0 8.4
STARTUP
(1 blank cycle) 2’28’’ 55.2 2.0 3.0 2.0 0.95
SHUT DOWN 2’56’’ 32.2 1.0 1.0 14.2 0.49
Rinse
CYTOMETER 1’14’’ 5.0 - - - -
AUTOCLEAN 1’33’’ 27.6 1.0 1.0 1.0 0.50
MINICLEAN 0’21’’ 10.3 1.0 2.0 1.0 0.33
CONCENTRA-
TED cleaning 4’00’’ 29.6 1.0 1.0 1.0 0.50
BACKFLUSH 0’24’’ - - - - -

SECTION 1 SPECIFICATIONS
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STARTUP cycle estimated duration and consumptions are given for one blank cycle control. It
could be a maximum of three cycles.
3. Summary of performance data
3.1. Repeatability
• Based on 10 consecutive samples of the same fresh whole blood sample without alarm:
3.2. Linearity
3.3. Carryover
• Carry-over was tested by analyzing samples with hight concentration of WBCs, RBCs, Hgb and
PLTs. Each sample was run in triplicate, followed by three background cycles. The % carryover is
calculated using the following formula:
Table 4: Repeatibility
PARAMETERS %CV Test Level
WBC < 2% at 10 x 103/µL
RBC < 2% at 5 x 106/µL
Hgb < 1% at 15 g/dL
Hct < 2% at 45%
Plt < 5% at 300 x 103/µL
Table 5: Linearity
PARAMETERS Linearity Range Difference
(Which ever is greater)
WBC (103/µL) 0 to 120 x 103/µL +/- 0.3 or +/- 7%
RBC (106/µL) 0 to 8 x 106/µL +/- 0.07 or +/- 2%
RBC (106/µL) 8 to 18 x 106/µL Reportable range
PLT (103/µL) 0 to 1 900 x 103/µL +/- 10 or +/- 7%
PLT Concentrated mode (103/µL) 1500 to 5000 x 103/µL +/- 10 or +/- 7 %
HGB (g/dl) 1.3 to 24 g/dl +/- 0.3 or +/- 2%
HCT (%) 2 to 67 % +/- 2 or +/- 3%
Table 6: Carryover
WBC RBC Hgb PLT
Level 43.64 8.56 25.94 739
% Carryover (Actual) 0.00 0.00 0.05 0.28
% Carryover (Claimed) <0.5% <0.5% <0.5% <0.5%
Carryover Background1 Background3–
Sample3 Background3–
-------------------------------------------------------------------------x100=

PENTRA 80 TECHNICAL MANUAL RAA022B
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4. Reagents specification
In order for the instrument to operate correctly, hight-quality reagents must be used.
ABX DIAGNOSTICS provides all the necessary reagents.
All these reagents have been registered by the A.F.S.S.A.P.S. «Agence Française de Sécurité
Sanitaire des Produits de Santé» according to the procedure relative to laboratory reagents
used for biological analyses.
These reagents are used for in vitro diagnostics.
All these reagents are manufactured by:
ABX DIAGNOSTICS
Rue du caducée - Parc Euromédecine
34184 MONTPELLIER CEDEX - FRANCE
Tel: [phone] - Fax: [phone]
4.1. ABX DILUENT
• Function: This diluent is necessary for the process involved in counting (and differentiating) the
blood cells. This reagent is used also to rinse the hydraulic parts of the instrument.
• Composition: Stabilized saline solution which contains an organic buffer, an antiseptic and
Sodium Azide < 0.1%.
• Description: Limpid and odourless aqueous solution.
• Physico-chemical properties: Boiling point: About 100°C, pH: neutral.
• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Handling Precautions: Avoid skin and eye contact. Use laboratory gloves when handling the
reagents. If a large quantity of reagent is ingested a mucous irritation can result.
• Emergency First aid: If the eyes or skin come into contact with the reagent, rinse thoroughly with
water. If a large quantity is ingested, drink water immediately, and induce vomiting.
• Storage conditions: Stored at 18°C (65°F) to 25°C (77°F) away from light.
• ABX DIAGNOSTICS Part number: 0901020
4.2. ABX LYSE
• Function: This reagent is used to lyse blood cells and determine hemoglobin concentration.
• Composition:The reagent contains potassium cyanide at 0.03%, a quarternary ammonium salt
and a saline phosphate buffer containing sodium azide < 0.1%.
• Description: Aqueous solution, limpid.
• Physico-chemical properties: Boiling point: approximately 100°C. pH: basic, smells of cyanide.
• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Handling Precautions: May be dangerous. Avoid contact with eyes, skin and clothing. Wear
laboratory gloves when handling the product. The product may be harmful if ingested. The product
can be absorbed through an open wound, or inhalation.
• Emergency First aid: If the eyes or skin come into contact with the reagent, rinse with water. If
the reagent is inhaled, breathe fresh air immediately. If a large quantity is ingested, drink water
immediately, and induce vomiting. Call anti-poison center, or contact doctor.
• Storage conditions: Stored at 18°C (65°F) to 25°C (77°F) away from light. Product will degrade if
exposed to air, keep cap / probe assembly securely tightened.
• ABX DIAGNOSTICS Part number: 0906004
4.3. ABX BIOLYSE
• Function: This reagent is used to lyse blood cells and determine hemoglobin concentration.
• Composition:Quarternary ammonium chloride.

SECTION 1 SPECIFICATIONS
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• Description: Colorless, odorless.
• Physico-chemical properties: pH: 6.65
• Handling Precautions: May be dangerous. Avoid contact with eyes, skin and clothing. Wear
laboratory gloves when handling the product. The product may be harmful if ingested.
• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Emergency First aid: If the eyes or skin come into contact with the reagent, rinse with water. If
the product is ingested, call immediately a doctor.
• Storage conditions: Stored at 18°C (65°F) to 25°C (77°F) away from light..
• ABX DIAGNOSTICS Part number: 0906005
4.4. ABX CLEANER
• Function: Washing agent.
• Composition: Enzymatic solution with proteolytic action.
• Description: Transparent liquid.
• Physico-chemical properties: Boiling point: around 100°C. pH: 9.6
• Handling Precautions: May be harmful. Avoid contact with eyes, skin and clothing.
• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Emergency First Aid: In case the product comes into contact with the eyes, rinse with water. If
the product is ingested, call a doctor immediately.
• Storage conditions: Stored at 18°C (65°F) to 25°C (77°F).
• ABX DIAGNOSTICS Part number: 0903010
4.5. ABX EOSINOFIX
• Function: This reagent lyses RBCs, fixes leukocytes and gives a specific coloration to
eosinophils.
• Composition: Alcoholic solution containing propylene-glycol, a formic dye, buffers, alkaline salts,
wetting agents and an aldehyde preservative.
• Description: Deep blue aqueous solution, smells of alcohol.
• Physico-chemical properties: pH: 6.9
• Handling precautions: Avoid contact with eyes, skin and clothing. Wear laboratory gloves when
handling the product. The product may be harmful if ingested or inhalted. Keep the bottle closed
when not in use.
• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Emergency First Aid: If the eyes or skin come into contact with the reagent, rinse with water. If
the reagent is inhaled or ingested, call local anti-poison center or contact doctor.
• Storage conditions: Room temperature between 18°C (65°F) to 25°C (77°F).
• ABX DIAGNOSTICS Part number: 0206010
4.6. ABX BASOLYSE II
• Function: This reagent lyses RBCs for the leukocytes and differential count of the polynuclear
basophils.
• Composition: Acidic solution containing a lytic agent.
• Description: Colorless aqueous solution.
• Physico-chemical properties: pH: 2.4
• Handling precautions: Avoid contact with eyes, skin and clothing. Wear laboratory gloves when
handling the product. The product may be harmful if ingested or inhalated. Keep the bottle closed
when not in use.

PENTRA 80 TECHNICAL MANUAL RAA022B
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• Mesuring Principles: See user manual.
• Performances: See user manual.
• Results: See user manual.
• Directions for use: See user manual.
• Emergency First Aid: If the eyes or skin come into contact with the reagent, rinse with water. If
the reagent is inhaled, breath fresh air immediately. If a large quantity is ingested, drink water
immediately. Do not induce vomiting. Call local anti-poison center or contact doctor.
• Storage conditions: Room temperature between 18°C (65°F) to 25°C (77°F).
• ABX DIAGNOSTICS Part number: 0906003
4.7. Waste handling precautions
If required, waste can be neutralized before being discarded. Follow your laboratory’s protocol
when neutralizing and disposing of waste.
5. Limitations
5.1. Maintenance
In Chapter 8. Maintenance, specific maintenance procedures are listed. The maintenance
procedures identified are mandatory for proper use and operation of the ABX PENTRA 80.
Failure to execute any of these recommended procedures may result in poor reliability of the
system.
5.2. Blood specimens
Verification of any abnormal test result (including flagged results or results outside of the normal
range) should be performed using reference methods or other standard laboratory procedures for
conclusive verification of the results. The sections below list known limitations of automated blood cell
counters which use the principles of impedance and light absorbance as principles of measurement.
5.3. Known interfering substances
WBC:
• White Blood Cells (Leukocytes):WBC results that exceed the linearity limits of the system will
require dilution of the blood sample (Leukemia sample followed by a leukopenia). Re-assaying the
diluted sample will help to obtain the correct assay value.
• Unlysed Red Cells - In some rare instances, the erythrocytes in the blood sample may not be
completely lysed. These non-lysed red blood cells may be detected on the WBC histogram with an
L1 alarm or as an elevated baseline on the side (leading edge) of the lymphocytes population. Non-
lysed erythrocytes will cause a falsely elevated WBC count.
• Multiple myeloma - The precipitation of proteins in multiple myeloma patients may give high
WBC counts.
• Leukemia - A very low WBC count may result from this disease because of possible increased
fragility of the leukocytes leading to destruction of some of these cells during counting. These white
cell fragments will also interfere with the white cell differential parameters.
• Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of the
leukocytes which may cause low WBC counts.
• Cryoglobulins - Increased levels of cryoglobulin that may be associated with myeloma,
carcinoma, leukemia, macroglobulinemia, lymphoproliferative disorders, metastic tumors,
autoimmune disorders, infections, aneurism, pregnancy, thromboembolic phenomena, diabetes,
etc, which can increase the WBC, RBC or Plt counts and the Hgb concentration. The specimen
must be warmed up to 37°C (99°F) in a bain marie for 30 minutes and analyzed again immediately
after (analyzer or manual method).
• Macrothrombocytes - in excessive numbers may affect and increase Leukocyte numeration.

SECTION 1 SPECIFICATIONS
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RBC:
• Red Blood Cells (Erythrocytes): The red blood cell dilution contains all the formed elements in
the blood: erythrocytes, leukocytes and platelets. During erythrocytes counting (red blood cells),
platelets are not counted as their size falls below the minimum threshold.
• Agglutinated erythrocytes - May cause a low incorrect RBC count. Blood samples containing
the agglutinated red blood cells may be suspected by elevated MCH and MCHC values and shown
by examination of the stained blood film.
• Cold agglutinins - IgM immunoglobulins which are high in cold agglutinin disease may cause
lower RBC and Plt counts and increase MCV.
Hgb (Hemoglobin):
• Turbidity of the blood sample - Any number of physiological and/or therapeutic factors may
produce high incorrect Hgb results. To obtain accurate hemoglobin results when increased turbidity
of the blood sample occurs, determine the cause of the turbidity and follow the appropriate method
below:
• High WBC: An extremely high WBC will cause excessive light scatter. In these cases use
reference (manual) methods.The diluted sample should be centrifuged, and the supernatant fluid
measured with a spectrophotometer.
• High lipid concentration: A high concentration of lipids in the blood sample will give the plasma
a «milky» appearance. This condition can occur with hyperlipidemia, hyperproteinemia (as in
gammapathies) and hyperbilirubinemia. Accurate hemoglobin determinations can be achieved by
using reference (manual) methods and a plasma blank.
• Increased turbidity may also be seen in cases where the red blood cells are resistant to lysing.
This condition will cause an incorrect high Hgb result, but may be detected by observing the
abnormal MCH, MCHC values, and the increased baseline on the leading edge of the WBC
histogram. Erroneous hemoglobin results will cause the results of the MCH and MCHC to be
incorrect as well.
• Fetal bloods - The mixing of fetal and maternal bloods may produce a high inaccurate Hgb value.
Hct (Hematocrit):
• Red blood cells agglutination - May produce an inaccurate Hct and MCV values. Red blood cell
agglutination may be detected by observing abnormal MCH and MCHC values, as well as by
examination of the stained blood film In such cases, manual methods may be required to obtain an
accurate Hct value
MCV (Mean Corpuscular Volume):
• Red blood cell agglutination - May produce an inaccurate MCV value. Red blood cell
agglutination may be detected by observing abnormal MCH and MCHC values, as well as by
examination of the stained blood film. In such cases, manual methods may be required to obtain an
accurate MCV value.
• Excessive numbers of large platelets and/or the presence of an excessively high WBC count
may interfere with the accurate determination of the MCV value. In such cases, careful examination
of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin):
• The MCH is determined according to Hgb value and the RBC count. The limitations listed for the
Hgb and RBC will have an effect on the MCH and may cause inaccurate values.
MCHC (Mean Corpuscular Hemoglobin Concentration):
• The MCHC is determined according to the Hgb and Hct values. The limitations listed for the Hgb
and Hct will have an effect on the MCHC and may cause inaccurate values.
RDW (Red blood cell Distribution Width):
• The red blood cell distribution width is determined according to the RBC count.
• Nutritional deficiency or blood transfusion - May cause high RDW results due to iron and/or
cobalamin and /or folate deficiency.
Plt (Platelets):
• Very small erythrocytes (microcytes), erythrocyte fragments (schizocytes) and WBC fragments
may interfere with the proper counting of platelets and cause elevated Plt counts.
• Agglutinated erythrocytes - May trap platelets, causing an erroneously low platelet count. The
presence of agglutinated erythrocytes may be detected by observation of abnormal MCH and
MCHC values and by careful examination of the stained blood film.
• Giant platelets in excessive numbers - may cause a low inaccurate platelet count as these large

PENTRA 80 TECHNICAL MANUAL RAA022B
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platelets may exceed the upper threshold for the platelet parameter and are not counted.
• Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of these
cells which may cause low Plt counts. Reference (manual) methods may be necessary to obtain an
accurate platelet count.
• Hemolysis - Hemolysed specimens contain red cell stroma which may increase platelet counts.
• A.C.D. blood - Blood anticoagulated with acid-citrate-dextrose may contain clumped platelet
which could decrease the platelet count.
• Platelet agglutination - Clumped platelets may cause a decreased platelet count and/or a high
WBC count. The specimen should be recollected in sodium citrate anticoagulant to ensure the
anticoagulated character depending on agglutination and reanalyzed only for the platelet count.
The final Plt result must be corrected for the sodium citrate dilution effect. However, these platelet
clumps do trigger flags L1, LL and LL1.
MPV (Mean Platelet Volume):
• Giant platelets that exceed the upper threshold of the Platelet parameter may not be counted as
platelets. Consequently, these larger platelets will not be included in the instrument’s calculation of
Mean Platelet Volume.
• Very small erythrocytes (microcytes), erythrocytic fragments (Schizocytes) and white blood cell
fragments may interfere with the proper counting and sizing of Platelets.
• Agglutinated erythrocytes - May trap Platelets, causing an incorrect MPV result. The presence
of agglutinated erythrocytes may be detected by observation of abnormal MCH and MCHC values
and by careful examination of the stained blood film.
• Chemotherapy - May also affect the sizing of Plts.
Blood samples collected in EDTA will not maintain a stable Mean Platelet Volume. Platelets
collected in EDTA swell depending on the time post-collection and storage temperature.
LYM# (Lymphocyte count absolute value), LYM% (Lymphocyte percentage):
• The Lymphocyte count is derived from the WBC count. The presence of erythroblasts, certain
parasites and erythrocytes that are resistant to lysis may interfere with an accurate LYM count.
Limitations listed for the WBC count pertain to the LYM # and % counts as well.
MON# (mononuclear cell count absolute), MON% (Mononuclear percentage):
• The mononuclear cell count absolute is derived from the WBC count. The presence of large
lymphocytes, atypical lymphocytes, blasts and an excessive number of basophils may interfere
with an accurate monocyte count.
• Limitations listed for the WBC count pertain to the MON # and % counts as well.
NEU# (neutrophil count absolute), NEU% (Neutrophil percentage):
• The neutrophils cell count is derived from the WBC cell count. The excessive presence of
eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere
with an accurate neutrophils count.
EOS# (Eosinophil cell count absolute), EOS% (Eosinophil percentage):
• The eosinophil cell count is derived from the WBC cell count. The presence of abnormal granules
(degranulated areas, toxic granules...) may interfere with the eosinophil counting.
BAS# (Basophil cell count absolute), BAS% (Basophil percentage):
• The Basophil cell count is derived from the WBC cell count.

Hydraulic & Pneumatic principles
Section 2: Hydraulic & Pneumatic principles
1. Hydropneumatic connections..................................2-2
2. Instrument tubing ....................................................2-5
2.1. Tube list ...................................................................... 2-5
2.2. Connectors and integrated tubings list ......................... 2-7
3. Function of valves ...................................................2-7
4. Hydraulic circuit .....................................................2-8
4.1. Cleaner circuit ............................................................. 2-9
4.2. Basolyse 2 circuit ...................................................... 2-10
4.3. Eosinofix circuit......................................................... 2-11
4.4. Lyse circuit ................................................................ 2-12
4.5. Diluent circuit ........................................................... 2-13
4.6. Diluent prime circuit ................................................. 2-14
4.7. Counting circuit......................................................... 2-15
4.8. LMNE transfer circuit ................................................. 2-16
4.9. LMNE injection circuit............................................... 2-17
5. Pneumatic diagram................................................2-18

PENTRA 80 TECHNICAL MANUAL RAA022B
2/18
1. Hydropneumatic connections
Read this table as follow: LV3_2 means connection to port 2 of valve 3.
Table 1: Hydropneumatic connection
Circuit From F.S . Diameter Length T.S. To
AIR Atmosphere 2.05 140 LV14_2
LV14_ 1 1.52 70 Count syringe_1
Rinse chamber_1 -
Atmos. 2.05 550 LV17_2
LV17_ 1 1.52 30 T8_1
Atmosphere 2.05 80 LV19_1
LV19_ 3 1.52 500 Diluent reservoir_2
LV19_ 2 1.52 60 T8_3
T8_2 1.52 230 Drain syringe # 2_1
Atmosphere 2.05 70 LV21_2
LV21_ 1 1.30 185 Isolator_2
Rinse chamber_2 -
Atmos. 2.05 300 LV24_2
LV24_ 1 1.52 90 Drain syringe # 1_1
DILUENT REA-
GENT
ABX Diluent contai-
ner C 3x6 maxi. 2000 Diluent input
Diluent input 2.54 190 SLV29_2
LV29_ 1 S2.54 180 SDiluent reservoir_4
2.05 65 Diluent reservoir_1
Diluent reservoir_1 2.05 370 LV2_1
LV2_3 S2.05 120 LMNE syringe_2
LV3_2 S1.52 50 SLV3 _3
LV3_1 S1.52 60 SLV1 _3
LV1_1 S1.52 200 SIsolator_1
Isolator_2 S1.52 100 T1_1
T1_2 1.14 35 LMNE flow cell_4
T1_2 Xba403a LMNE flow cell_4
T1_3 1.14 35 LMNE flow cell_2
T1_3 Xba403a LMNE flow cell_2
LV1_2 1.52 300 T6_3
LV2_2 1.02 175 LMNE syringe_4
LMNE syringe_1 1.02 205 LMNE flow cell_5
LMNE flow cell_7 1.02 10 (Cap)
2.05 65 Diluent reservoir_3
Diluent reservoir_3 2.05 610 LV10_1
LV10_ 3 2.05 160 Reagent syringe_4
LV10_ 2 1.52 50 LV11_3
LV11_ 1 1.52 15 T5_2
T5_1 1.52 760 LV22_3
LV22_ 1 1.52 50 LV23_3
LV23_ 1 1.52 190 T3_1
T3_3 1.52 75 Probe rinse block_1
T3_2 1.30 15 Isolator_1
Isolator_3 1.52 215 LV20_1

SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES
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LV20_2 1.52 760 T7_1
LV23_2 1.52 300 Probe rinse block_2
LV22_2 1.02 115 Sample syringe_2
LV11_2 1.52 440 LV25_3
LV25_1 1.52 65 LV27_3
LV27_1 1.52 50 LV26_3
LV26_1 1.52 200 SReagent heater_7
Reagent heater_8 S1.52 160 RBC chamber_1
LV26_2 1.52 260 SReagent heater_1
Reagent heater_2 S1.52 160 DIL1/HGB chamber_1
LV27_2 1.52 50 T10_2
LV25_2 1.52 170 WBC/Baso chamber_4
ABX CLEANER
REAGENT Abx Cleaner bottle 2.05 800 LV8_1
LV8_3 2.05 160 Reagent syringe_2
LV8_2 1.52 70 LV6_3
LV6_1 1.52 300 T10_3
T10_1 1.52 180 WBC/Baso chamber_1
LV6_2 1.52 120 T5_3
ABX EOSINO-
FIX REAGENT Abx Eosinofix bottle 2.05 800 LV9_1
LV9_3 2.05 160 Reagent syringe_3
LV9_2 1.52 550 SReagent heater_3
Reagent heater_4 S1.52 160 LMNE chamber_3
ABX BASO-
LYSE II REA-
GENT
Abx Basolyse II
bottle 2.05 800 LV12_1
LV12_3 2.05 160 Reagent syringe_5
LV12_2 1.52 575 SReagent heater_10
Reagent heater_9 S1.52 60 SReagent heater_11
Reagent heater_12 S1.52 160 WBC/Baso chamber_2
ABX ALPHA-
LYSE REA-
GENT
Abx Alphalyse bottle 1.52 800 LV7_1
LV7_3 1.52 160 Reagent syringe_1
LV7_2 1.52 480 DIL1/HGB chamber_2
SAMPLING Probe_1 1.02 205 Sample syringe_1
Probe_1 SSample syringe_1
LMNE COUN-
TING LMNE chamber_4 1.30 20 M4_2 photocell
M4_1 photocell 1.30 320 LV4_1
LV4_2 1.02 130 T2_2
T2_3 1.02 250 LMNE syringe_5
LMNE syringe_3 1.14 85 T4_1
T4_2 1.02 15 LV5_1
LV5_2 1.52 280 T7_2
T2_1 S1.85 10 LMNE flow cell_6
0.19 4LMNE flow cell_6
Table 1: Hydropneumatic connection
Circuit From F.S. Diameter Length T.S. To

PENTRA 80 TECHNICAL MANUAL RAA022B
4/18
LMNE flow
cell_output 1.52 20 E1_1 anode fitting
E1_2 anode fitting 1.52 70 Isolator_1
Isolator_2 1.52 80 E2_1 ground fitting
E2_2 ground fitting 1.52 335 LV28_2
LV28_ 1 1.52 120 T6_2
T6_1 1.52 160 SReagent heater_5
Reagent heater_6 S1.52 160 LMNE chamber_2
WBC/RBC
COUNTING
WBC/Baso
chamber_3 1.52 200 RBC chamber_3
RBC chamber_2 1.52 480 LV15_2
LV15_ 1 1.52 70 Count syringe_2
WASTE Rinse chamber_3 2.05 20 Filter_1
Filter_2 2.05 100 LV31_2
LV31_ 1 1.52 30 T12_3
DIL1/HGB
chamber_3 1.52 65 LV32_2
LV32_ 1 1.52 15 T12_2
T12_1 1.52 35 T13_3
RBC chamber_4 1.52 65 LV34_2
LV34_ 1 1.52 15 T13_2
T13_1 1.52 180 M2_1 photocell
M2_2 photocell 1.52 15 Isolator # 2_1
Isolator # 2_2 2.05 280 LV30_1
LV30_ 3 S2.54 160 SDrain syringe # 1_2
LV30_ 2 S2.54 150 T14_3
LMNE chamber_5 1.52 65 LV33_2
LV33_ 1 1.52 60 E3_1
E3_2 1.52 60 T11_1
T7_3 1.52 100 T11_3
T11_2 1.52 15 M1_1 photocell
M1_2 photocell 1.52 15 Isolator # 1_1
Isolator # 1_2 2.05 500 LV18_1
LV18_ 3 2.05 210 Drain syringe # 2_2
LV18_ 2 S2.54 150 T9_2
WBC/Baso
chamber_5 1.52 50 LV35_2
LV35_ 1 1.52 130 M3_1 photocell
M3_2 photocell 1.52 15 Isolator # 3_1
Isolator # 3_2 2.05 460 Count syringe_3
T4_3 1.02 150 LV13_1
LV13_ 2 2.05 140 Count syringe_4
Count syringe_5 2.05 120 LV16_1
LV16_ 2 S2.54 150 T9_3
T9_1 2.54 225 T14_2
T14_1 2.54 20 Waste output
Waste output C 4x6 maxi. 2000 Waste container
Table 1: Hydropneumatic connection
Circuit From F.S . Diameter Length T.S. To

SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES
5/18
2. Instrument tubing
2.1. Tube list
Table 2: Tube list
Designation Part Number Diameter Length Quantity Comment
Y Connector EAB021A 3 2
Y Connector EAB026A 2.5 2
T410-6 Connector EAB033A 1.6 2
T220-6 Connector EAB035A 2.3 8
0.040 TYGON
tubing EAE005A 1.02 10 1
1.02 15 1
1.02 85 1
1.02 115 1
1.02 130 1
1.02 150 1
1.02 175 1
1.02 205 2
1.02 240 1
0.051 TYGON
tubing EAE006A 1.30 15 1
1.30 20 1
1.30 185 1
1.30 320 1
0.060 TYGON
tubing EAE007A 1.52 15 7
1.52 20 1
1.52 30 2
1.52 35 1
1.52 50 6
1.52 60 5
1.52 65 4
1.52 70 4
1.52 75 1
1.52 80 1
1.52 90 1
1.52 100 2
1.52 120 2
1.52 130 1
1.52 160 7
1.52 170 1
1.52 180 2
1.52 190 1
1.52 200 3
1.52 215 1
1.52 230 1
1.52 260 1

PENTRA 80 TECHNICAL MANUAL RAA022B
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1.52 280 1
1.52 300 3
1.52 335 1
1.52 440 1
1.52 480 1
1.52 500 1
1.52 550 1
1.52 575 1
1.52 760 2
1.52 800 1
0.081 TYGON
tubing EAE008A 2.05 20 1
2.05 65 2Notched tubing
2.05 70 1Notched tubing
2.05 80 1Notched tubing
2.05 100 1
2.05 120 2
2.05 140 1
2.05 140 1Notched tubing
2.05 160 4
2.05 210 1
2.05 280 1
2.05 300 1
2.05 370 1
2.05 460 1
2.05 500 1
2.05 550 1
2.05 610 1
2.05 800 3
CRISTAL tubing EAE011A 3x6 maxi
2000 1
CRISTAL tubing EAE028A 4x6 maxi
2000 1
0.045 TYGON
tubing EAE033A 1.14 35 2
0.100 TYGON
tubing EAE034A 2.54 20 1
2.54 150 3
2.54 160 1
2.54 180 1
2.54 190 1
2.54 225 1
0.0075 TYGON
tubing EAE047A 0.19 4 1
0.073 TYGON
tubing EAE048A 1.85 10 1
Sleevings GAL098A 30 S2x4 Lg=6 tubing
EAE026A
Tube shielding XBA403A 2
Table 2: Tube list
Designation Part Number Diameter Length Quantity Comment