Horiba Scientific FluoroMax-4 User manual

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
i
FluoroMax®-4 &
FluoroMax®-4P
with USB
Operation Manual
http://www.HORIBA.com
Rev. D

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
ii
Copyright © 2011, 2012 by HORIBA Instruments Incorporated. All rights reserved. No
part of this work may be reproduced, stored, in a retrieval system, or transmitted in any
form by any means, including electronic or mechanical, photocopying and recording,
without prior written permission from HORIBA Instruments Incorporated. Requests for
permission should be requested in writing. Origin®is a registered trademark of
OriginLab Corporation. Alconox®is a registered trademark of Alconox, Inc. Ludox®is
a registered trademark of W.R. Grace and Co. Teflon®is a registered trademark of E.I.
du Pont de Nemours and Company. Windows®is a trademark of Microsoft Corpora-
tion. Uniblitz®is a registered trademark of VA, Inc.
Information in this manual is subject to change without notice, and does not represent a
commitment on the part of the vendor.
July 2012
Part Number J810005

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
iii
Table of Contents
0: Introduction ........................................................................................... 0-1
About the FluoroMax®-4 and FluoroMax®-4P ..................................................................................0-1
Chapter overview .............................................................................................................................0-2
Disclaimer.........................................................................................................................................0-3
Safety summary ...............................................................................................................................0-5
Risks of ultraviolet exposure ............................................................................................................0-7
Additional risks of xenon lamps........................................................................................................0-9
CE compliance statement..............................................................................................................0-11
1: Requirements & Installation ........................................................................ 1-1
Safety-training requirements............................................................................................................1-1
Surface requirements.......................................................................................................................1-2
Environmental requirements............................................................................................................1-3
Electrical requirements.....................................................................................................................1-4
Unpacking and Installation...............................................................................................................1-5
Software emulation ..........................................................................................................................1-9
2: System Description ................................................................................... 2-1
Introduction...................................................................................................................................... 2-1
Basic theory of operation .................................................................................................................2-1
Optical layout....................................................................................................................................2-2
3: System Operation...................................................................................... 3-1
Introduction.......................................................................................................................................3-1
Power switch....................................................................................................................................3-1
Turning on the system......................................................................................................................3-2
Checking system performance.........................................................................................................3-3
4: Data Acquisition ....................................................................................... 4-1
Introduction to FluorEssence™ and Multigroup ...............................................................................4-1
Experiment Menu button..................................................................................................................4-2
Previous Experiment Setup button ..................................................................................................4-4
Auto Run Previous Experiment button.............................................................................................4-5
Make Overlay File button.................................................................................................................4-6
3D Scan to 3D Profile button............................................................................................................4-7
Run JY Batch Experiments button...................................................................................................4-9
Real Time Control ..........................................................................................................................4-11
Create/Use Calibration Curve from CWA Data..............................................................................4-12
2D Intensity Map ............................................................................................................................4-16
Launch DataStation........................................................................................................................4-18
Switch menu between HJY Software Application and Origin Std..................................................4-19
Multigroup.......................................................................................................................................4-20
Running an unknown sample.........................................................................................................4-23
Using corrected signals in FluorEssence™ ...................................................................................4-28
5: Optimizing Data....................................................................................... 5-1
Cuvette preparation..........................................................................................................................5-1
Sample preparation..........................................................................................................................5-2
Running a scan on a sample............................................................................................................5-4
Measuring the Gfactor.....................................................................................................................5-5
Improving the signal-to-noise ratio...................................................................................................5-7
Correcting data...............................................................................................................................5-14
6: Maintenance............................................................................................ 6-1
Introduction.......................................................................................................................................6-1
Lamp replacement ...........................................................................................................................6-1
Electronics......................................................................................................................................6-11
Updating the FluoroMax®-4 firmware.............................................................................................6-13

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
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7: Troubleshooting........................................................................................ 7-1
Chart.................................................................................................................................................7-1
Using diagnostic spectra..................................................................................................................7-4
Further assistance… .......................................................................................................................7-9
8: Producing Correction Factors ....................................................................... 8-1
Introduction.......................................................................................................................................8-1
Generating emission correction factors ...........................................................................................8-2
Calculating emission correction factors ...........................................................................................8-3
Using correction-factor files............................................................................................................8-10
Excitation correction factors...........................................................................................................8-13
9: FluoroMax®-4P Phosphorimeter Operation ....................................................... 9-1
Introduction...................................................................................................................................... 9-1
Theory of operation..........................................................................................................................9-2
Applications for the phosphorimeter ................................................................................................9-5
Operation of the phosphorimeter .................................................................................................... 9-8
Processing phosphorimeter data ...................................................................................................9-10
Lamp replacement .........................................................................................................................9-11
10: Automated Polarizers...............................................................................10-1
Introduction.....................................................................................................................................10-1
Installation......................................................................................................................................10-5
Alignment .......................................................................................................................................10-6
Using automated polarizers .........................................................................................................10-13
Maintenance.................................................................................................................................10-18
Troubleshooting ...........................................................................................................................10-19
11: Technical Specifications ..........................................................................11-1
Introduction.....................................................................................................................................11-1
Spectrofluorometer system............................................................................................................11-2
Minimum host-computer requirements ..........................................................................................11-4
Software.........................................................................................................................................11-4
12: Components & Accessories .......................................................................12-1
Itemized list of FluoroMax®-4 accessories.....................................................................................12-2
FL-1013 Liquid Nitrogen Dewar Assembly ....................................................................................12-3
Sample cells...................................................................................................................................12-4
F-3026 Correction Factor Kit..........................................................................................................12-6
F-3023 Janis cryostat.....................................................................................................................12-7
F4-3000 Fiber Optic Mount and 1950 Fiber Optic Bundles ...........................................................12-8
1939 Cut-On Filter..........................................................................................................................12-9
FM-2008 Cut-On Filter Holder......................................................................................................12-10
FL4-1011 Four-Position Thermostatted Cell Holder....................................................................12-11
FL4-1012 Dual-Position Thermostatted Cell Holder....................................................................12-13
FL4-1027 Single-Position Thermostatted Cell Holder .................................................................12-15
J1933 Solid Sample Holder..........................................................................................................12-17
F-3005/6 Autotitration Injector......................................................................................................12-19
1905-OFR 150-W Xenon Lamp ...................................................................................................12-20
F-3004 Sample Heater/Cooler Peltier Thermocouple Drive ........................................................12-21
MicroMax 384 Microwell Plate Reader ........................................................................................12-22
FM4-2000 L-Format Polarizer......................................................................................................12-23
FL4-1015 Injector Port .................................................................................................................12-24
F-3029 Quanta-φaccessory........................................................................................................12-25
FM-2002 Shutter accessory.........................................................................................................12-26
F-3025 Stopped-flow accessory .................................................................................................12-27
FM4-2015 and FM4-2016 TCSPC upgrade.................................................................................12-28
F4-3030 Temperature Bath..........................................................................................................12-29
F-3031 Transmission Accessory..................................................................................................12-30
J400981 Trigger Cable.................................................................................................................12-31
FM-2007 Windows for the FluoroMax®-4 sample compartment..................................................12-32
13: Glossary ............................................................................................. 13-1

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
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14: Bibliography .........................................................................................14-1
15: Compliance Information ...........................................................................15-1
Declaration of Conformity...............................................................................................................15-1
Supplementary Information............................................................................................................15-1
16: Index ..................................................................................................16-1

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012)
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FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) Introduction
0-1
Note: Keep this and the other reference manuals near the system.
0: Introduction
About the FluoroMax®-4 and FluoroMax®-
4P
Both the FluoroMax®-4 and FluoroMax®-4P are self-contained, fully automated
spectrofluorometer systems. Data output is viewed on a PC, while printouts may be ob-
tained via an optional plotter or printer. All FluoroMax®-4 and FluoroMax®-4P func-
tions are under the control of FluorEssence™ spectroscopy software. The main parts of
the FluoroMax®-4 and FluoroMax®-4P spectrofluorometer systems are:
State-of-the-art optical components
A personal computer
FluorEssence™ for Windows®, the driving software.
The difference between the FluoroMax®-4 and FluoroMax®-4P is that the FluoroMax®-
4P contains a phosphorimeter for phosphorescence measurements. This manual ex-
plains how to operate and maintain a FluoroMax®-4 and FluoroMax®-4P
spectrofluorometer. The manual also describes measurements and tests essential to ob-
tain accurate data. For a complete discussion of the almost limitless power provided by
FluorEssence™, refer to the FluorEssence™ User’s Guide (especially regarding soft-
ware installation) and the on-line help for Origin®and FluorEssence™, which accom-
pany the system.

FluoroMax®-4 & FluoroMax®-4P with USB rev. D (30 Jul 2012) Introduction
0-2
Chapter overview
1: Requirements & Installation
Power and environmental requirements; select the best spot
for the instrument.
2: System Description
How the FluoroMax®-4 and -4P work.
3: System Operation
Operation of the spectrofluorometer system, and calibration
instructions.
4: Data Acquisition
How to use the special FluorEssence™ buttons to acquire
and plot data; how to determine peaks in an unknown sam-
ple.
5: Optimizing Data
Hints for improving the signal-to-noise ratio, instructions
for obtaining corrected data, and other information useful
for optimizing data and ensuring reproducibility.
6: Maintenance
Routine maintenance procedures such as replacing the lamp.
7: Troubleshooting
Potential sources of problems, their most probable causes,
and possible solutions.
8: Producing Correction Factors
How to correct for variation in the system’s sensitivity
across the spectral range.
9: FluoroMax®-4P
Phosphorimeter Operation
Theory, operation, applications, and troubleshooting of the
phosphorimeter, available only on the FluoroMax®-4P sys-
tem.
10: Automated Polarizers
Installation, operation, and troubleshooting of the optional
automated polarizers.
11: Technical Specifications
Instrument specifications and computer requirements.
12: Components & Accessories
Accessories available for the FluoroMax®-4, and how to use
them.
13: Glossary
Some useful technical terms related to fluorescence spec-
troscopy.
14: Bibliography
Other important sources of information.
15: Declaration of Conformity
16: Index