horiba ABX MICROS ES60 User manual

Explore the future
ABX Micros ES60
HAN706A © 2007 HORIBA ABX
User manual
P/n: RAB237AEN

User Manual
P/n: RAB237AEN
HORIBA ABX SAS
B.P. 7290
34184 MONTPELLIER Cedex 4 - FRANCE
ABX MICROS ES60 OT/CT

User Manual - RAB237AEN
User manual
Revisions
◆This document applies to the latest software version as indicated above.
◆When a subsequent software version is released, only the electronic version (CD-ROM and/or
online help) of this user manual is updated and supplied by HORIBA ABX. To update a paper
document, please contact your local HORIBA ABX Technical Support Representative.
Intended use
◆The ABX Micros ES60 OT/CT is a fully automated (microprocessor controlled) hematology analyser
used for in-vitro diagnostics testing of whole blood specimens and whole blood component
concentrates.
◆The ABX Micros ES60 OT/CT is available in 16 and 18 parameters. These parameters are noted
according to the system setup.
◆The rate of determination is approximately 60 samples per hour* in the optimum configuration.
The system is totally automated, including an internal dilution system, and a graphic printer for
recording all test results including flags and graphics.
*Throughput performance is reachable in optimized conditions with an admitted margin for variation
of 10%.
Notice of liability
◆The Information in this manual is distributed on an "As Is" basis, without warranty. While every
precaution has been taken in the preparation of this manual, HORIBA ABX will not assume any
liability to any persons or entities with respect to loss or damage, caused or alleged to be caused
directly or indirectly by not following the instructions contained in this manual, or by using the
computer software and hardware products described herein in a manner inconsistent with our
product labeling.
Declaration of conformity
◆The ABX Micros ES60 OT/CT responds to the Standards and directives named in the Declaration of
Conformity. The latest version of the CE declaration of conformity for this system is available on
www.horiba-abx.com.
Potential hazards
◆To alert the operator of potentially hazardous conditions, one of the bold captioned headings
which are described below is provided wherever necessary throughout this text.
Part number Technical note Software version Section Date
RAB237AEN V1.1.X All 13/02/09
Important: when analyzing whole blood component concentrates, you must
consider the linear range of the component parameter and its associated
parameters if any. These concentrates may prematurely pollute the counting
aperture when analyzing them. It is suggested that you perform 3 backflushes
and/or a concentrated cleaning after analyzing the concentrates.
Flags a procedure that if not followed properly, can prove to be extremely
hazardous to either the operator or the environment or both.
Emphasizes an operating procedure that must be followed to avoid possible
damage to the instrument or erroneous test results.
Emphasizes important information especially helpful to the operator before,
during or after a specific operational function.

User Manual - RAB237AEN
Graphics
◆All graphics including screens, printouts and photographs are for illustration purposes only and
are not contractual.
Trademarks
◆Microsoft and Windows are registered trademarks of Microsoft Corporation.
◆Other product names mentioned within this publication may be registered trademarks of other
companies.
Copyright 2008 HORIBA ABX SAS
All rights reserved. No part of this book may be reproduced or transmitted in any form or by any
means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written
permission of HORIBA ABX SAS.
HORIBA ABX SAS
Rue du Caducée - Parc Euromédecine - BP 7290
34184 MONTPELLIER Cedex 4 - FRANCE
Phone: [phone]
Fax: [phone]

User Manual - RAB237AEN

Contents
User Manual - RAB237AEN 1
Introduction
1. Warning and precautions .....................................................................................1-3
1.1. Recommendations ..........................................................................................1-3
1.2. Limited guarantee ...........................................................................................1-3
1.3. Safety Precautions ...........................................................................................1-4
1.3.1. Electronic and moving parts.......................................................................1-4
1.3.2. Biological risks ..........................................................................................1-4
2. Operational conditions ........................................................................................1-5
2.1. Environment....................................................................................................1-5
2.2. Location..........................................................................................................1-5
2.3. Grounding ......................................................................................................1-5
2.4. Humidity/temperature conditions....................................................................1-5
2.5. Electromagnetic environment check................................................................1-5
2.6. Main supply ....................................................................................................1-5
3. Environmental protection.....................................................................................1-6
3.1. Disposal of used accessories and consumables ...............................................1-6
3.2. Disposal of ABX Micros ES60 OT/CT instrument .............................................1-6
3.3. European Legislation .......................................................................................1-6
3.4. Transportation and storage conditions .............................................................1-6
4. Graphics and symbols ..........................................................................................1-7
5. Labels ...................................................................................................................1-8
5.1. Instrument rear view labels..............................................................................1-8
5.1.1. Identification label.....................................................................................1-8
5.1.2. Output label ..............................................................................................1-8
5.1.3. Supply module label..................................................................................1-9
5.1.4. Diluent and waste connection label ..........................................................1-9
5.2. "Biological risks" label...................................................................................1-10
5.3. Internal label.................................................................................................1-10
6. Presentation .......................................................................................................1-11
7. Software overview..............................................................................................1-12
7.1. Generic screen..............................................................................................1-12
7.2. Menu access buttons description...................................................................1-12
7.3. "Exit" button and "Exit" screen........................................................................1-12
7.4. "Status" button description.............................................................................1-13
7.5. "Contextual help" button description .............................................................1-14
7.6. Contextual toolbar ........................................................................................1-14
7.6.1. Action buttons .........................................................................................1-14
7.6.2. Virtual keyboard ......................................................................................1-14
7.7. Data transmission buttons .............................................................................1-15
7.8. Miscellaneous ...............................................................................................1-16
7.8.1. Navigation buttons ..................................................................................1-16
7.8.2. Dialog box...............................................................................................1-16
7.8.3. Check box ...............................................................................................1-17
7.8.4. Combo box..............................................................................................1-18
7.8.5. Radio button............................................................................................1-18
7.8.6. Text field .................................................................................................1-18
8. Installation .........................................................................................................1-19
8.1. Package contents ..........................................................................................1-19
8.1.1. Package contents check...........................................................................1-19
8.1.2. ABX Micros ES60 "Bottle" installation kit..................................................1-19

ABX Micros ES60 OT/CT
2 User Manual - RAB237AEN
8.1.3. ABX Micros ES60 "Pack" installation kit ...................................................1-20
8.2. Reagent installation.......................................................................................1-20
8.3. Instrument connectors...................................................................................1-21
8.4. Printer...........................................................................................................1-22
8.4.1. Integrated ticket printer............................................................................1-22
8.4.2. External printer (optional) ........................................................................1-22

Contents
User Manual - RAB237AEN 3
Specifications
1. Specifications .......................................................................................................2-2
1.1. Introduction ....................................................................................................2-2
1.2. Parameters ......................................................................................................2-2
1.3. Instrument specifications.................................................................................2-4
1.4. Technical specifications ..................................................................................2-5
2. Summary of performance data .............................................................................2-6
2.1. Precision (Reproducibility) ..............................................................................2-6
2.2. Precision (Repeatability) ................................................................................2-10
2.3. Linearity........................................................................................................2-10
2.4. Carry-over.....................................................................................................2-11
2.5. Normal ranges ..............................................................................................2-11
2.6. Accuracy.......................................................................................................2-11
3. Limitations .........................................................................................................2-12
3.1. Maintenance .................................................................................................2-12
3.2. Blood specimens...........................................................................................2-12
3.2.1. Sample collection and mixing..................................................................2-12
3.2.2. Sample stability .......................................................................................2-12
3.2.3. Anti-coagulants and their effects (on whole blood) ..................................2-13
3.2.4. Sample collection tube caps (ABX Micros ES60 CT).................................2-13
3.3. Known interfering substances........................................................................2-13
3.3.1. WBC White Blood Cells (Leukocytes) ......................................................2-13
3.3.2. RBC Red Blood Cells (Erythrocytes) .........................................................2-14
3.3.3. HGB (Hemoglobin)..................................................................................2-14
3.3.4. HCT (Hematocrit) ....................................................................................2-15
3.3.5. MCV (Mean Corpuscular Volume) ...........................................................2-15
3.3.6. MCH (Mean Corpuscular Hemoglobin) ...................................................2-15
3.3.7. MCHC (Mean Corpuscular Hemoglobin Concentration) ..........................2-15
3.3.8. RDW (Red cell Distribution Width)..........................................................2-15
3.3.9. PLT (Platelets) ..........................................................................................2-15
3.3.10. MPV (Mean Platelet Volume).................................................................2-16
3.3.11. LYM# (lymphocyte count absolute value) ..............................................2-16
3.3.12. LYM% (lymphocyte percentage) ............................................................2-16
3.3.13. MON# (monocyte count absolute value) ...............................................2-16
3.3.14. MON% (monocyte percentage) .............................................................2-16
3.3.15. GRA# (granulocyte count absolute value) ..............................................2-16
3.3.16. GRA% (granulocyte percentage) ............................................................2-16
4. Reagent ..............................................................................................................2-17
4.1. Reagent specifications...................................................................................2-17
4.2. Reagent consumption....................................................................................2-17
5. Waste handling procedure .................................................................................2-18

ABX Micros ES60 OT/CT
4 User Manual - RAB237AEN
Description & Technology
1. ABX Micros ES60 OT/CT description ...................................................................3-2
1.1. ABX Micros ES60 CT description ....................................................................3-2
1.1.1. Overview .................................................................................................. 3-2
1.1.2. Front view (covers opened)........................................................................ 3-2
1.1.3. Left side view (covers opened)...................................................................3-2
1.2. ABX Micros ES60 OT description....................................................................3-3
1.2.1. Overview .................................................................................................. 3-3
1.2.2. Front view (door opened) ..........................................................................3-3
1.2.3. Left side view (covers opened)...................................................................3-3
2. Software menus description.................................................................................3-4
3. Technology ..........................................................................................................3-5
3.1. Sampling.........................................................................................................3-5
3.1.1. ABX Micros ES60 CT .................................................................................3-5
3.1.2. ABX Micros ES60 OT.................................................................................3-5
3.2. Dilutions.........................................................................................................3-5
3.2.1. WBC/HGB ................................................................................................3-5
3.2.2. RBC/PLT.................................................................................................... 3-6
3.3. CBC measurement principles ..........................................................................3-7
3.3.1. RBC/PLT.................................................................................................... 3-7
3.3.2. HGB..........................................................................................................3-9
3.3.3. HCT ..........................................................................................................3-9
3.3.4. MCV, MCH & MCHC................................................................................3-9
3.3.5. RDW .........................................................................................................3-9
3.3.6. MPV ..........................................................................................................3-9
3.3.7. PCT ...........................................................................................................3-9
3.3.8. PDW .......................................................................................................3-10
3.3.9. WBC .......................................................................................................3-10