horiba FluoroMax-3 User manual

FluoroMax-3 v. 3.1 (3 Mar 2006)
i
FluoroMax®-3 & FluoroMax®-P
with FluorEssence™
Operation Manual
http://www.jobinyvon.com
Rev. 3.1
USA: HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ 08820-3012, Toll-Free: +1-866-jobinyvon
Tel: [phone], Fax: [phone], E-mail: [email protected], www.jobinyvon.com
France: HORIBA Jobin Yvon S.A.S., 16-18, rue du Canal, 91165 Longjumeau Cedex,
Tel: [phone]
, Fax: [phone], www.jobinyvon.fr
Japan: HORIBA Ltd., JY Optical Sales Dept, Higashi-Kanda, Daiji Building, 1-7-8 Higashi-Kanda
Chiyoda-ku, Tokyo 101-0031, Tel: [phone], www.jyhoriba.jp
Germany: [phone] Italy: [phone] UK: [phone]
China: [phone]
(All HORIBA Jobin Yvon companies were formerly known as Jobin Yvon)

FluoroMax-3 v. 3.1 (3 Mar 2006)
ii
Copyright © 2001–2006 by HORIBA Jobin Yvon
Inc.
All rights reserved. No part of this work may be
reproduced, stored, in a retrieval system, or
transmitted in any form by any means, including
electronic or mechanical, photocopying and
recording, without prior written permission from
HORIBA Jobin Yvon Inc. Requests for permission
should be requested in writing. Origin®is a registered
trademark of OriginLab Corporation. Alconox®is a
registered trademark of Alconox, Inc. Ludox®is a
registered trademark of W.R. Grace and Co. Teflon®
is a registered trademark of E.I. du Pont de Nemours
and Company.
Information in this manual is subject to change
without notice, and does not represent a commitment
on the part of the vendor.
April 2006
Part Number 81038

FluoroMax-3 v. 3.1 (3 Mar 2006)
iii
Table of Contents
0: Introduction ................................................................................................ 0-1
About the FluoroMax®-3 and FluoroMax®-P...........................................................................................0-1
Chapter overview....................................................................................................................................0-2
Disclaimer...............................................................................................................................................0-3
Safety summary......................................................................................................................................0-5
Risks of ultraviolet exposure...................................................................................................................0-7
Additional risks of xenon lamps............................................................................................................0-10
1: Requirements & Installation ............................................................................ 1-1
Safety-training requirements ..................................................................................................................1-1
Surface requirements.............................................................................................................................1-2
Environmental requirements...................................................................................................................1-3
Electrical requirements...........................................................................................................................1-4
Unpacking and Installation .....................................................................................................................1-5
Software emulation.................................................................................................................................1-9
2: System Description....................................................................................... 2-1
Introduction.............................................................................................................................................2-1
Basic theory of operation........................................................................................................................2-1
Optical layout..........................................................................................................................................2-2
3: System Operation ......................................................................................... 3-1
Introduction.............................................................................................................................................3-1
Controls and indicators...........................................................................................................................3-1
Turning on the system............................................................................................................................3-2
Checking system performance...............................................................................................................3-3
4: Data Acquisition........................................................................................... 4-1
Experiment Menu button ........................................................................................................................4-2
Previous Experiment Setup button.........................................................................................................4-4
Auto Run Previous Experiment button ...................................................................................................4-5
Make Overlay File button........................................................................................................................4-6
3D Scan to 3D Profile button..................................................................................................................4-7
Get Peak Information button...................................................................................................................4-9
Running an unknown sample...............................................................................................................4-10
5: Optimizing Data ........................................................................................... 5-1
Cuvette preparation................................................................................................................................5-1
Sample preparation ................................................................................................................................5-2
Running a scan on a sample..................................................................................................................5-4
Measuring the Gfactor...........................................................................................................................5-6
Improving the signal-to-noise ratio .........................................................................................................5-8
Correcting data.....................................................................................................................................5-14
6: Maintenance............................................................................................... 6-1
Introduction.............................................................................................................................................6-1
Lamp replacement..................................................................................................................................6-1
Electronics..............................................................................................................................................6-9
7: Troubleshooting........................................................................................... 7-1
Chart.......................................................................................................................................................7-1
Checking the FluoroMax®’s BIOS...........................................................................................................7-3
Using diagnostic spectra ........................................................................................................................7-5
Further assistance… ............................................................................................................................7-10

FluoroMax-3 v. 3.1 (3 Mar 2006)
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8: Producing Correction Factors ........................................................................... 8-1
Introduction.............................................................................................................................................8-1
Generating emission correction factors..................................................................................................8-2
Calculating emission correction factors..................................................................................................8-3
Calculating excitation correction factors.................................................................................................8-5
Using correction-factor files..................................................................................................................8-10
9: FluoroMax®-P Phosphorimeter Operation ............................................................ 9-1
Introduction.............................................................................................................................................9-1
Theory of operation ................................................................................................................................9-2
Applications for the phosphorimeter.......................................................................................................9-5
Operation of the phosphorimeter............................................................................................................9-8
Processing phosphorimeter data..........................................................................................................9-10
Lamp replacement................................................................................................................................9-11
10: Automated Polarizers..................................................................................10-1
Introduction...........................................................................................................................................10-1
Installation.............................................................................................................................................10-5
Alignment..............................................................................................................................................10-6
Using automated polarizers................................................................................................................10-14
Maintenance.......................................................................................................................................10-19
Troubleshooting..................................................................................................................................10-20
11: Technical Specifications ..............................................................................11-1
Spectrofluorometer system...................................................................................................................11-2
Minimum computer requirements.........................................................................................................11-4
Software................................................................................................................................................11-4
12: Components & Accessories...........................................................................12-1
Itemized list of FluoroMax®-3 accessories ...........................................................................................12-2
Model 1940 Absorption/Transmission Accessory................................................................................12-3
FL-1013 Liquid Nitrogen Dewar Assembly...........................................................................................12-6
Model 1908MOD Scatter Block Assembly ...........................................................................................12-7
Model 1908 Standard Lamp Assembly ................................................................................................12-7
Sample cells .........................................................................................................................................12-8
F-3000 Fiber Optic Mount.....................................................................................................................12-9
Model 1938 Cut-On Filter...................................................................................................................12-10
Model 1939 Cut-On Filter...................................................................................................................12-10
FL-1010 Cut-On Filter Holder.............................................................................................................12-11
FL-1011 Four-Position Thermostatted Cell Holder ............................................................................12-12
FL-1012 Dual-Position Thermostatted Cell Holder ............................................................................12-14
Model 1933 Solid Sample Holder.......................................................................................................12-16
F-3005/6 Autotitration Injector............................................................................................................12-18
Microscope Interface..........................................................................................................................12-19
Model 1905-OFR 150-W Xenon Lamp...............................................................................................12-20
F-3004 Sample Heater/Cooler Peltier Thermocouple Drive ..............................................................12-21
FM-2005 Phosphorimeter upgrade ....................................................................................................12-22
MicroMax 384 Microwell Plate Reader...............................................................................................12-23
Fl-1044 L-Format Polarizer.................................................................................................................12-24
FL-1015 Injector Port..........................................................................................................................12-25
Quantum-Yield accessory ..................................................................................................................12-26
SFA-20/SPEX Stopped-flow accessory .............................................................................................12-27
FM-2013 TCSPC upgrade..................................................................................................................12-28
F-1001/1 Temperature Bath...............................................................................................................12-29
Model TRIG-15/25 External Trigger Accessory .................................................................................12-30
FM-2007 Windows for the FluoroMax®-3 sample compartment ........................................................12-31
13: Glossary .................................................................................................13-1
14: Bibliography ............................................................................................14-1

FluoroMax-3 v. 3.1 (3 Mar 2006)
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15: Declaration of Conformity........................................................................ 15-1
16: Index .................................................................................................... 16-1

FluoroMax-3 v. 3.1 (3 Mar 2006)
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FluoroMax-3 v. 3.1 (3 Mar 2006) Introduction
0-1
Note: Keep this and the other reference
manuals near the system.
0: Introduction
About the FluoroMax®-3 and FluoroMax®-P
Both the FluoroMax®-3 and FluoroMax®-P are self-contained, fully automated spectro-
fluorometer systems. Data output is viewed on a PC, while printouts may be obtained
via an optional plotter or printer. All FluoroMax®-3 and FluoroMax®-P functions are
under the control of FluorEssence™ spectroscopy software. The main parts of the
FluoroMax®-3 and FluoroMax®-P spectrofluorometer systems are:
•State-of-the-art optical components
•A personal computer
•FluorEssence™ for Windows™, the driving software.
The difference between the FluoroMax®-3 and FluoroMax®-P is that the FluoroMax®-P
contains a phosphorimeter for phosphorescence measurements. This manual explains
how to operate and maintain a FluoroMax®-3 and FluoroMax®-P spectrofluorometer.
The manual also describes measurements and tests essential to obtain accurate data. For
a complete discussion of the almost limitless power provided by FluorEssence™, refer
to the FluorEssence™ User’s Guide (especially regarding software installation) and the
on-line help for Origin®and FluorEssence™, which accompany the system.

FluoroMax-3 v. 3.1 (3 Mar 2006) Introduction
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Chapter overview
1: Requirements & Installation Power and environmental requirements;
select the best spot for the instrument.
2: System Description How the FluoroMax®-3 and -P work.
3: System Operation Operation of the spectrofluorometer system,
and calibration instructions.
4: Data Acquisition How to use the special FluorEssence™
buttons to acquire and plot data; how to
determine peaks in an unknown sample.
5: Optimizing Data Acquisition Hints for improving the signal-to-noise ratio,
instructions for obtaining corrected data, and
other information useful for optimizing data
and ensuring reproducibility.
6: System Maintenance Routine maintenance procedures such as
replacing the lamp.
7: Troubleshooting Potential sources of problems, their most
probable causes, and possible solutions.
8: Producing Correction Factors How to correct for variation in the system’s
sensitivity across the spectral range.
9: FluoroMax®-P Phosphorimeter Opera-
tion
Theory, operation, applications, and
troubleshooting of the phosphorimeter,
available on the FluoroMax®-P system.
10: Automated Polarizers Installation, operation, and troubleshooting
of the optional automated polarizers.
11: Technical Specifications Instrument specifications and computer
requirements.
12: Components & Accessories Accessories available for the FluoroMax®-3,
and how to use them.
13: Glossary Some useful technical terms related to
fluorescence spectroscopy.
14: Bibliography Other important sources of information.
15: Declaration of Conformity
16: Index

FluoroMax-3 v. 3.1 (3 Mar 2006) Introduction
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Disclaimer
By setting up or starting to use any HORIBA Jobin Yvon product, you are accepting
the following terms:
You are responsible for understanding the information contained in this document. You
should not rely on this information as absolute or all-encompassing; there may be local
issues (in your environment) not addressed in this document that you may need to ad-
dress, and there may be issues or procedures discussed that may not apply to your situa-
tion.
If you do not follow the instructions or procedures contained in this document, you are
responsible for yourself and your actions and all resulting consequences. If you rely on
the information contained in this document, you are responsible for:
•Adhering to safety procedures
•Following all precautions
•Referring to additional safety documentation, such as Material Safety Data Sheets
(MSDS), when advised
As a condition of purchase, you agree to use safe operating procedures in the use of all
products supplied by HORIBA Jobin Yvon, including those specified in the MSDS
provided with any chemicals and all warning and cautionary notices, and to use all
safety devices and guards when operating equipment. You agree to indemnify and hold
HORIBA Jobin Yvon harmless from any liability or obligation arising from your use or
misuse of any such products, including, without limitation, to persons injured directly
or indirectly in connection with your use or operation of the products. The foregoing
indemnification shall in no event be deemed to have expanded HORIBA Jobin Yvon’s
liability for the products.
HORIBA Jobin Yvon products are not intended for any general cosmetic, drug, food, or
household application, but may be used for analytical measurements or research in
these fields. A condition of HORIBA Jobin Yvon’s acceptance of a purchase order is
that only qualified individuals, trained and familiar with procedures suitable for the
products ordered, will handle them. Training and maintenance procedures may be pur-
chased from HORIBA Jobin Yvon at an additional cost. HORIBA Jobin Yvon cannot
be held responsible for actions your employer or contractor may take without proper
training.
Due to HORIBA Jobin Yvon’s efforts to continuously improve our products, all speci-
fications, dimensions, internal workings, and operating procedures are subject to
change without notice. All specifications and measurements are approximate, based on
a standard configuration; results may vary with the application and environment. Any
software manufactured by HORIBA Jobin Yvon is also under constant development
and subject to change without notice.
Any warranties and remedies with respect to our products are limited to those provided
in writing as to a particular product. In no event shall HORIBA Jobin Yvon be held li-

FluoroMax-3 v. 3.1 (3 Mar 2006) Introduction
0-4
able for any special, incidental, indirect or consequential damages of any kind, or any
damages whatsoever resulting from loss of use, loss of data, or loss of profits, arising
out of or in connection with our products or the use or possession thereof. HORIBA
Jobin Yvon is also in no event liable for damages on any theory of liability arising out
of, or in connection with, the use or performance of our hardware or software, regard-
less of whether you have been advised of the possibility of damage.