horiba ABX MICROS ES60 User manual

Technical Manual
P/n: RAA033AEN
HORIBA ABX SAS
B.P. 7290
34184 MONTPELLIER Cedex 4 - FRANCE
ABX MICROS ES60/ESV60

ABX Micros ES60/ESV60
2 - Technical Manual - RAA033AEN
Introduction
Revisions
◆This document applies to the latest software version as indicated above.
◆When a subsequent software version changes the information in this manual, a new section and/
or sections will be released.
Notice of liability
◆The Information in this manual is distributed on an «As Is» basis, without warranty. While every
precaution has been taken in the preparation of this manual, HORIBA Medical will not assume any
liability to any persons or entities with respect to loss or damage, caused or alleged to be caused
directly or indirectly by not following the instructions contained in this manual, or by using the
computer software and hardware products described herein in a manner inconsistent with our
product labeling.
Potential hazards
◆To alert the operator of potentially hazardous conditions, one of the bold captioned headings
which are described below is provided wherever necessary throughout this text.
Graphics
◆All graphics including screens and printouts, photographs are for illustration purposes only and
are not contractual.
Trademarks
◆Other product names mentioned within this publication may be registered trademarks of other
companies.
Copyright 2009 HORIBA Medical
All rights reserved. No part of this book may be reproduced or transmitted in any form or by any
means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written
permission of HORIBA Medical.
HORIBA Medical
Rue du Caducée - Parc Euromédecine
34184 MONTPELLIER Cedex 4 - FRANCE
Tel: + 33 (0)4 67 14 15 16
Fax: + 33 (0)4 67 14 15 17
Index P/n revision Software revision Section Date
ARAN033A V1.1.X All 12/02/09
Flags a procedure that if not followed properly, can prove to be extremely
hazardous to either the operator or the environment or both.
Emphasizes an operating procedure that must be followed to avoid possible
damage to the instrument or erroneous test results.
Emphasizes important information especially helpful to the operator before,
during or after a specific operational function.

Introduction
Technical Manual - RAA033AEN - 3
1. Operational conditions
1.1. Environment
◆The operation of the ABX Micros ES60/ESV60 should be restricted to indoor location use only.
Instrument is operational at an altitude of maximum 3000 meters (9840 feet).
◆The ABX Micros ES60/ESV60 is designed for safety from voltages surges according to INSTALLATION
CATEGORY II and POLLUTION DEGREE 2 (IEC 61010-1) Please contact your local HORIBA Medical
representative for information regarding operation locations, when it does not comply with the
recommended specifications.
1.2. Location
◆The ABX Micros ES60/ESV60 should be placed on a clean and leveled table or workbench.
◆Please note that the instrument, printer and reagents weigh approximately 20 kilograms (44 lbs).
◆Avoid exposure to sunlight.
◆Place your instrument in a well-ventilated area.
◆Place your instrument where it is not exposed to water or vapor.
◆Place your instrument where it is free from vibration or shock.
◆Place your instrument where an independent power receptacle can be used.
◆Use a receptacle different from the one used by a device that easily generate noise such as a
centrifuge, etc...
◆Provide a space of at least 20 cm (8 inches) at the back of the instrument for arranging the power
cable and tubings.
1.3. Grounding
◆Proper grounding is required when installing the system. Check the wall outlet ground (Earth) for
proper grounding to the facilities electrical ground. If you are unsure of the outlet grounding,
contact your facilities engineer to verify the proper outlet ground.
1.4. Humidity/temperature conditions
◆The ABX Micros ES60/ESV60 must operate between temperatures of 16°C to 30°C (61°F to 86°F).
◆Maximum relative humidity 85% for temperature up to 30°C (86°F) without condensation.
◆If it is stored at a temperature less than 10°C (50°F), the instrument should stand for 1 hour at
the correct room temperature before use.
1.5. Electromagnetic environment check
◆The ABX Micros ES60/ESV60 has been designed to produce less than the accepted level of
electromagnetic interference in order to operate in conformity with its destination, allowing the
correct operation of other instruments also in conformity with their destination.
◆In case of suspected electromagnetic noise, check that the instrument has not been placed in the
proximity of electromagnetic fields or short wave emissions, (i. e. Radar, X-rays, Scanners, Cell
phones, etc...).
The power switch and input voltage supply connection should always be
accessible. When positioning the system for operational use, leave the
required amount of space for easy accessibility to these items.

ABX Micros ES60/ESV60
4 - Technical Manual - RAA033AEN
1.6. Main supply
◆Grounding is required. Check that the earth wall-plug is correctly connected to the laboratory
grounding system. If there is no such system a ground stake should be used.
◆Use only main supply cable delivered with the ABX Micros ES60/ESV60.
◆Main supply voltage fluctuations must not exceed +/-10% of the nominal voltage.
2. Environmental protection
2.1. Disposal used accessories and consumables
◆Must be collected by a laboratory specialized in elimination and recycling of this kind of material
according to the local legislation.
2.2. Disposal ABX Micros ES60/ESV60 instrument
◆It should be disposed of, in accordance with local legislation, and should be treated as being
contaminated with blood. The appropriate biological precautions should be taken.
2.3. European Legislation
2.4. Transportation and storage conditions
◆Condition for storage and transportation: Temperature from -20°C to +65°C (-4°F to 122°F).
If any doubt, please contact your HORIBA Medical representative service
department.
In accordance with the European Directive (2002/96/CE, known also as
W.E.E.E) instruments having the above symbol and sold into a European
country by HORIBA Medical or an authorised representative must be disposed
of and recycled correctly at the end of its useful life.
Due to the local changing regulations in each country, please contact your
local representative for detailed and upto date information on how to
appropriately dispose of the instrument.
Prior to the shipping of an instrument by transporter, whatever the
destination, an external decontamination of the instrument must be carried
out.

Specifications
Technical Manual - RAA033AEN - S01 / 1
Sect ion 0 1: Spe cif icat ions
1. Specifications ...................................................................................................S01-2
1.1. Parameters................................................................................................S01-2
1.2. Instrument specifications ..........................................................................S01-3
1.3. Technical specifications............................................................................S01-3
2. Description ......................................................................................................S01-4
2.1. Overview..................................................................................................S01-4
2.1.1. ABX Micros ES60 CT ........................................................................S01-4
2.1.2. ABX Micros ES60 OT........................................................................S01-4
2.2. Front view (covers opened).......................................................................S01-4
2.2.1. ABX Micros ES60 CT ........................................................................S01-4
2.2.2. ABX Micros ES60 OT........................................................................S01-5
2.3. Left side view (covers opened) ..................................................................S01-5
2.4. Rear view .................................................................................................S01-5

ABX Micros ES60/ESV60
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1. Specifications
1.1. Parameters
- 16 parameters
- 18 parameters
Parameter Definition
WBC White blood cells
LYM% Lymphocyte Percentage
LYM# Lymphocyte Absolute number
MON% Monocyte Percentage
MON# Monocyte Absolute number
GRA% Granulocyte Percentage
GRA# Granulocyte Absolute number
RBC Red blood cells
HGB Hemoglobin
HCT Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red cell Distribution Width
PLT Platelets
MPV Mean Platelet Volume
WBC, RBC and PLT Distribution Curves
Parameter Definition
WBC White blood cells
LYM% Lymphocyte Percentage
LYM# Lymphocyte Absolute number
MON% Monocyte Percentage
MON# Monocyte Absolute number
GRA% Granulocyte Percentage
GRA# Granulocyte Absolute number
RBC Red blood cells
HGB Hemoglobin
HCT Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red cell Distribution Width
PLT Platelets
MPV Mean Platelet Volume
PDW* Platelet Distribution Width
PCT* Plateletcrit
WBC, RBC and PLT Distribution Curves
*PDW and PCT have not been established as indications for this product, in
the United States. The use of PDW and PCT should be restricted to Research
Use Only.

Specifications
Technical Manual - RAA033AEN - S01 / 3
1.2. Instrument specifications
1.3. Technical specifications
ABX MICROS ES60 (OT/CT)/ ABX MICROS ESV60 (OT)
Throughput analysis
Approximately 60 Samples/hour for the ABX Micros ES60 OT, 50
Samples/hour for the ABX Micros ES60 CT and 50 Samples /hour for
the ABX Micros ESV60.
Minimum sample volume Minimum blood sample requirement 50µl
Analyzer sample volume 10µl
Dilution ratios
WBC Approximately 1/260 for ABX Micros ES60 and 1/255 for ABX
Micros ESV60
RBC/PLT Approximately 1/15000
Measurements and
Computation
Impedance change for WBC, RBC, PLT.
Spectrophotometry for HGB.
Impedance change for LYM%, MON%, GRA%.
Computation from stored Data that was directly measured for MCV,
MCH, MCHC, RDW, MPV, LYM#, MON#, GRA#.
Counting Aperture Diameter WBC: 80µm / RBC: 50µm.
Hemoglobin Measurement Performed in the WBC/HGB Chamber.
Light source LED (Light Emiting Diode) at wavelength 550nm.
Statistics and Quality Control Extended Quality Control package.
Calibration Automatic Calibration procedure.
Direct entering of Calibration Coefficients.
Reagents
ABX Micros ES60:
3 Reagents or 1 Pack of Reagents:
Diluent: ABX Minidil LMG (10L)
Cleaner: ABX Miniclean (1L) or ABX Cleaner (0.5L)
Lyse: ABX Minilyse LMG (1L), ABX Alphalyse (0.4L) or ABX
Alphalyse 360 (0.36L)
Pack all reagents: ABX Minipack LMG (4.2L)
ABX Micros ESV60: ABX VetPack
Wastes Automatic disposal
Waste handling according to Local/National regulations
ABX MICROS ES60 (OT/CT)/ ABX MICROS ESV60 (OT)
Software Designed by HORIBA Medical, Installed on a Flash EPROM
Memory capacity 1000 results
Display Operated touch screen, LVDS Screen: 8’’4, 640x480, 256000 colours
Outputs Hard Copy printing (Internal or external printer)
External output (RS232)
Barcode reader
C 39, C 128, ITF (2of5), CODABAR, ISBT C128 (for ABX Micros ES60
CT only).
(External barcode reader optional for ABX Micros ES60)
Internal ticket printer ABX Micros ES60 only
Power requirements
Power supply 100V, 240V (+/- 10%) 50/60Hz.
Power Consumption
Maximum: 150VA (-30%, +10%)
In use: 100VA (-30%, +10%)
Stand-by mode: 35 VA (-30%, +10%)
Heat output 197Kj/h (187BTU/h)
Dimensions
Height Approximately 430mm (16.9 inches)
Width Approximately 360mm (14.2 inches)
Depth Approximately 360mm (14.2 inches)
Weight Weight Approximately 17Kgs (37.5 lbs)

ABX Micros ES60/ESV60
S01 / 4 - Technical Manual - RAA033AEN
2. Description
2.1. Overview
2.1.1. ABX Micros ES60 CT
2.1.2. ABX Micros ES60 OT
2.2. Front view (covers opened)
2.2.1. ABX Micros ES60 CT
1- LCD display touchscreen
2- Cap piercing mechanism & Tube Holder
3- Reagent compartment
4- Printer
5- Barcode reader
6- USB port
1- LCD display & touchscreen
2- Manual sampling needle
3- Reagent compartment
4- Printer
5- USB port
1- Carriage assembly
2- Tube holder
3- WBC/HGB chamber
4- RBC chamber
1
5
2
3
4
6
1
3
2
4
5
2
1
4
3

Specifications
Technical Manual - RAA033AEN - S01 / 5
2.2.2. ABX Micros ES60 OT
2.3. Left side view (covers opened)
2.4. Rear view
1- Carriage assembly
2- Sampling needle and analysis start bar
3- WBC/HGB chamber
4- RBC chamber
1- Diluent temperature sensor
2- Liquid syringe
3- Valve blocks
4- Vacuum/waste syringe
1- 2 RS232 ports
2- 2 USB ports
3- 1 Jack connector: not functional
4- 1 PS2 ports
5- 1 RJ45 port
6- 1 power supply
7- 1 Diluent input connector
8- 1 Waste output connector
2
1
4
3
4
2
3
1
12
3
4
5
78
6

ABX Micros ES60/ESV60
S01 / 6 - Technical Manual - RAA033AEN