HTA HT2000H User manual

HT2000H_en_n.doc
User Guide
HT2000H, HT2100H, HT2000HT
Static Headspace GC Autosamplers
www.hta-it.com

HT2000H_en_n.doc 16/01/2018
This manual contains information about the product(s) indicated on the cover of this document.
The information contained in this document is subject to change without notice.
This document is for information only, the manufacturer accepts no liability for errors contained
herein or for incidental or consequential damage with the furnishing, performance, or use of this
material.
Unless otherwise specified, each reference to names or belongings is purely casual and has the
sole purpose of illustrating the product described here. Contents of this publication may not be
reproduced in any form or by any means (including electronic storage and retrieval or translation
into a foreign language), without prior agreement and written consent from the copyright owner.
All rights are reserved.
Manufacturer: HTA s.r.l. - 77-79, via del Mella, I-25131 Brescia (BS), Italy - Tel: [phone] -
E-mail: [e-mail]

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Table of Contents
1 INTRODUCTION .......................................................................................................................8
1.1 I NTENDED USE AND RESTRICTIONS ..........................................................................................8
1.2 S YMBOL TABLE .....................................................................................................................9
1.3 W ARNING ...........................................................................................................................10
1.4 S TANDARDS C OMPLIANCE ...................................................................................................13
1.5 G ENERAL SPECIFICATION .....................................................................................................14
1.6 F UNCTIONS AVAILABLE ONLY THROUGH HTA A UTOSAMPLER M ANAGER ....................................14
1.7 V ERSIONS ..........................................................................................................................15
1.7.1 Description...................................................................................................................................... 15
1.7.2 Options............................................................................................................................................ 15
1.8 T ECHNICAL S PECIFICATIONS ................................................................................................16
1.9 PC SOFTWARE REQUIREMENTS (HTA A UTOSAMPLER M ANAGER ).............................................19
2 EQUIPMENT DESCRIPTION...................................................................................................20
2.1 P ARTS D EFINITION ..............................................................................................................20
2.1.1 HT2000H/HT2000HT Overview .....................................................................................................20
2.1.2 HT2100H Overview ........................................................................................................................ 25
2.1.3 Syringe location .............................................................................................................................. 28
2.1.4 Connection panel ............................................................................................................................ 30
2.2 M OVING PARTS ...................................................................................................................32
2.3 T OOL KIT ............................................................................................................................33
2.4 T OUCH SCREEN DISPLAY DESCRIPTION (HT2000H/HT2000HT) ..............................................34
2.4.1 Touchscreen use.............................................................................................................................. 34
2.4.2 Screen templates ............................................................................................................................. 35
2.4.3 Limited Warranty ............................................................................................................................ 42
3 INSTALLATION .......................................................................................................................43
3.1 U NPACKING AND PRELIMINARY OPERATIONS ..........................................................................43
3.2 A UTOSAMPLER ASSEMBLY ...................................................................................................44
3.2.1 How to lift and carry the autosampler ............................................................................................ 47
3.3 I NSTALLATION .....................................................................................................................48
3.3.1 Autosampler installation on the mounting plate ............................................................................. 48
3.3.2 Safety Lock setting .......................................................................................................................... 53
3.3.3 Electrical connections..................................................................................................................... 54
3.3.4 Purge line connections.................................................................................................................... 55
3.4 S TART -U P ..........................................................................................................................56

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3.5 PC CONNECTION AND SOFTWARE INSTALLATION .....................................................................56
3.5.1 Autosampler controlled by LAN-connected PC ..............................................................................57
3.5.2 Autosampler connected directly to the PC ...................................................................................... 65
3.6 A CCESSORIES INSTALLATION ................................................................................................66
3.6.1 External pressure regulator installation ......................................................................................... 67
3.6.2 Swagelok Adapter installation ........................................................................................................69
3.6.3 System integrity tool installation.....................................................................................................70
3.7 P REPARING SAMPLE VIAL .....................................................................................................72
3.7.1 Vial capping (Crimping Cap).......................................................................................................... 73
3.8 F INAL INSTALLATION OPERATIONS .........................................................................................74
3.9 B ASIC TRAINING ..................................................................................................................74
4 SET UP OPERATIONS............................................................................................................76
4.1 V IEW P ARAMETERS .............................................................................................................76
4.2 S ETUP ...............................................................................................................................77
4.2.1 Setup: Alignment.............................................................................................................................77
4.2.2 Setup: Syringe warmer assembly installation .................................................................................80
4.2.3 Setup: Manual operations............................................................................................................... 89
4.2.4 Setup: Configuration.......................................................................................................................92
4.2.5 Setup: Service.................................................................................................................................. 98
5 PROGRAMMING .....................................................................................................................99
5.1 HT2100H: M ETHODS AND S EQUENCE ..................................................................................99
5.2 HT2000H/HT2000HT: M ETHODS ........................................................................................99
5.2.1 Method Menu .................................................................................................................................. 99
5.2.2 Method X: Setting method parameters............................................................................................99
5.2.3 Method tools.................................................................................................................................. 103
5.2.4 Example ........................................................................................................................................ 103
5.3 HT2000H/HT2000HT: S EQUENCE ....................................................................................105
5.3.1 Sequence Menu ............................................................................................................................. 105
5.3.2 Setting sequence step parameters ................................................................................................. 105
5.3.3 Sequence tools............................................................................................................................... 106
6 HT2100H OPERATIONS .......................................................................................................107
6.1 K EYS ...............................................................................................................................107
6.1.1 Keys Settings ................................................................................................................................. 108
6.2 S TATUS I NDICATORS .........................................................................................................108
6.2.1 Reference table.............................................................................................................................. 108

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7 HT2000H/HT2000HT OPERATIONS .....................................................................................111
7.1 H OME SCREEN ..................................................................................................................111
7.1.1 Home screen: Standard User Interface......................................................................................... 111
7.1.2 Home screen: Quick Start User Interface ..................................................................................... 112
7.2 S INGLE INJECTION .............................................................................................................112
7.3 A UTOMATIC RUN ...............................................................................................................113
7.3.1 Interrupting an automatic run....................................................................................................... 114
7.3.2 Immediate Injection....................................................................................................................... 114
7.3.3 Skip Next Vial option..................................................................................................................... 114
7.4 S YRINGE PURGE ...............................................................................................................115
7.5 L OAD AND U NLOAD S AMPLE V IAL .......................................................................................115
7.6 S ETTINGS M ENU ...............................................................................................................115
7.6.1 Change tray/rack type................................................................................................................... 116
7.6.2 Eco Savings ................................................................................................................................... 117
8 TROUBLESHOOTING ...........................................................................................................119
8.1 I NTRODUCTION .................................................................................................................119
8.2 S TART UP ERRORS ............................................................................................................120
8.2.1 Safety lock error............................................................................................................................ 120
8.2.1 Vial locator error ...................................................................................................................... 120
8.2.2 Memory error................................................................................................................................ 120
8.3 M ETHOD ERRORS .............................................................................................................121
8.3.1 Fill Volume/Pull Up Strokes settings incompatibility ................................................................... 121
8.3.1 Fill volume/Syringe Pre-Fill settings incompatibility ............................................................... 121
8.4 T OUCH S ENSOR PROBLEMS ...............................................................................................122
8.5 F ALLING VIALS ..................................................................................................................122
8.6 O BSTACLE FOUND .............................................................................................................123
8.7 O BSTACLE FOUND DURING COVER MOVEMENT .....................................................................123
8.8 M ISMATCH ERRORS ...........................................................................................................124
8.8.1 Single injection mismatch error .................................................................................................... 124
8.8.2 Automatic run mismatch errors .................................................................................................... 124
8.9 M ISSING ERRORS ..............................................................................................................126
8.9.1 Missing: front injector alignment .................................................................................................. 126
8.9.2 Missing: rear injector alignment .................................................................................................. 126
8.9.3 Missing: touch & plunger zero...................................................................................................... 126
8.10 T IMEOUT ERROR ...............................................................................................................127
8.10.1 Peripheral communication timeout ........................................................................................... 127

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8.11 M ECHANICAL ERRORS .......................................................................................................127
8.12 H EATING ERRORS .............................................................................................................128
8.13 O VEN ERROR ...................................................................................................................128
8.14 S YSTEM INTEGRITY TEST FAIL .............................................................................................128
8.15 D AMAGE TO SYRINGE NEEDLE ............................................................................................129
8.15.1 Syringe inspection ..................................................................................................................... 130
8.16 T RAY EMERGENCY RELEASE ...............................................................................................131
8.16.1 Tray opening by hand ............................................................................................................... 131
8.17 “HTA A UTOSAMPLER M ANAGER ” TROUBLESHOOTING ............................................................133
8.17.1 Autosampler and PC do not communicate (connection by LAN)............................................. 133
8.18 A NALYTICAL TROUBLESHOOTING .........................................................................................135
8.18.1 Reproducibility issue................................................................................................................. 135
8.18.2 Peak distortion or tailing .......................................................................................................... 136
8.18.3 Carry over issue........................................................................................................................ 136
8.18.4 Unexplained chromatographic peaks ....................................................................................... 136
8.18.5 Retention times not repeatable.................................................................................................. 136
8.18.6 Faulty temperature read-out..................................................................................................... 137
9 MAINTENANCE.....................................................................................................................138
9.1 O RDINARY MAINTENANCE ..................................................................................................138
9.1.1 Cleaning of vial rack/tray ............................................................................................................. 138
9.1.2 External cleaning of cabinet ......................................................................................................... 139
9.1.3 Incubation oven cleaning .............................................................................................................. 139
9.1.4 Touch screen display cleaning ...................................................................................................... 140
9.1.5 Syringe replacement in the syringe warmer assembly .................................................................. 140
9.1.6 Septum of the System integrity tool replacement....................................................................... 150
9.1.7 Preventive maintenance pack.................................................................................................... 151
9.2 E XTRAORDINARY MAINTENANCE .........................................................................................151
10 UNINSTALLING AND DISPOSAL.......................................................................................152
10.1 A UTOSAMPLER UNINSTALLING ............................................................................................152
10.2 A UTOSAMPLER SHIPPING ...................................................................................................153
10.2 A UTOSAMPLER DISPOSAL ...................................................................................................156
10.3 D ISPOSAL INSTRUCTIONS ...................................................................................................156
11 APPENDIX A – GLOSSARY ..............................................................................................158
12 APPENDIX B – CONSUMABLES .......................................................................................160
12.1 S YRINGES ........................................................................................................................160

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12.1.1 How to select the correct syringe type (only for HT2000HT)................................................... 160
12.2 S AMPLE VIALS ..................................................................................................................161
12.2.1 Vial specifications..................................................................................................................... 161
12.2.2 Cap specifications..................................................................................................................... 164
12.2.3 Septa specifications................................................................................................................... 164
13 APPENDIX C – CONNECTION PANEL..............................................................................165
13.1 GC CONNECTOR ...............................................................................................................165
13.1.1 Typical GC interface................................................................................................................. 166
13.2 RS232C INTERFACE ( OPTIONAL )........................................................................................166

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1 Introduction
1.1 Intended use and restrictions
HT2000H, HT2100H and HT2000HT are static headspace autosamplers for gas
chromatography.
Headspace gas chromatography (GC) is a technique used for the concentration and analysis of
volatile organic compounds in almost any sample matrix.
The autosampler must be used in a professional environment, and only by properly trained
users. The autosampler must be used according to the regulations in force regarding the safety
in testing laboratories.
A list (not exhaustive) of typical applications of the autosampler is reported below:
•Environmental analysis
•Quality control in the chemical and pharmaceutical industry
•Studies related to pharmaceuticals
•Drug discovery
•Other techniques or areas of application are possible.
The safety measures and precautions regarding use, maintenance and repair, are not intended
for applications that require the use of toxic or infectious substances.
Every possible mixture or omission of a component cannot be recognized or previously
prevented. The operator that will use the autosampler must be trained about the used
substances, their hazards, their use and their proper disposal. For the correct use of the used
substances, refer to chemicals safety data sheets (MSDS) and to the regulations, laws,
directives and safety decrees currently in force that regulate the behaviour inside the testing
laboratories.
The insertion and positioning of the sample (vial), the insertion of syringes and all the other
preparation activities are under the sole responsibility of the user. The user must operate in
accordance with the law, in particular the ones concerning the safety of testing laboratories and
accident prevention.
The operator must be trained to operate the equipment in accordance with the safety standards
for the laboratories. The autosampler does not have specific protection mechanisms against
substances that are corrosive, potentially infectious, toxic and radioactive or against other
substances that can be hazardous to health. However, when the autosampler is used, because

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of the handled substances, the user could need to use individual protection measures such as
gloves, masks and goggles; the use of such devices must comply with the regulations currently
in force regarding the safety of testing laboratories.
The use of the autosampler for any technique invasive for humans or animals is explicitly
prohibited. The autosampler must be used only in the manner described in the usage
instructions. Any other use may affect the autosampler and operator safety.
HTA s.r.l. Is not responsible for damage caused, even only in part, by improper use of the
autosampler, by unauthorized modification of it, by different or missing execution of the
procedures described in this manual, or by the use of the autosampler violating the applicable
laws and regulations.
The autosampler has been designed and tested in accordance with current safety standards.
1.2 Symbol table
Please carefully read this table before using your autosampler. These symbols are used in this
manual.
Generic warning
Attention: dangerous voltage
Attention: mechanical hazard
Biological risks (if applicable for the substances used with the autosampler)
Hot surface

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1.3 Warning
Read the following warnings before unpacking or using the unit.
See paragraph 1.1 “Intended use and restrictions” and be sure you have understood
it.
See EU Declaration of Compliance enclosed with the autosampler.
Before removing the autosampler from the box, please carefully read paragraphs 3.1
“Unpacking and preliminary operations” and 3.2 “Autosampler assembly”.
Before switching the autosampler ON check the correct voltage.
Electrical Hazards. Every analytical instrument has specific hazards, so ensure you
read and comply with the following precautions.
Use only the power supply system supplied with the autosampler. The use of a
different system could cause damage to the equipment and/or compromise safety.
There are no operator-serviceable or replaceable parts inside the power supply. In
case of failure, contact the Technical Assistance Centre. Violations of these directives
imply the immediate invalidation of the warranty and service contract.
The supplied power cord must be inserted into a power outlet with a protective earth
contact (ground). When using an extension cord, make sure that the cord also has an
earth contact.
Do not change the external or internal grounding connections. Tampering with or
disconnecting these connections could endanger you and/or damage the
autosampler.
The product is designed and tested in accordance with international safety standards.
You do not need to make any changes to the electrical connections or to the chassis
of the autosampler in order to ensure safe operation.
Do not turn the autosampler on if you suspect that it has incurred any kind of electrical
damage or if it shows visible signs of damage. If, for any reason, the safety
protections were compromised or the autosampler shows visible signs of damage,
disconnect the power cord and contact an HTA representative. The power cord
connector is considered to be the disconnecting device of the product. Electrical
damage may have occurred if the autosampler shows visible signs of damage, or has